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تاثير اضافة بعض الاحماض الامينية وعصير البنجر الى المخففات في بعض صفات السائل المنوي المبرد والمجمد للكباش == Effect of adding some amino acid and Beet juice to the dilutors on some cooling and freezing rams semen traits
Author name:
فلاح عبد المحسن عبد الله الاسدي
Supervisor name:
عماد فلاح حسن | عبد الرزاق نعيم خضير
General topic:
Agricultural sciences
Specific topic:
Zoology
Degree:
Doctorate
University:
University Of Basrah - Faculty Of Agriculture
Language:
Arabic
University location:
Basrah
First pages:
31T1847 - p.pdf
Abstract:
The present study was conducted at the agricultural experimental Research Station / College of Agriculture / University of Basrah form 3rd /1/2015 to 3rd /1/2016. A total of 12 mature rams (4 Najdi , 4 Arabi and 4 crosses) aged 3 - 3.5 years with nearly similar weights . Nutrition depended on concentrate ration ( 47 % wheat bran , 50 % barely ,1% urea , 1% CaCo3 and 1% Nacl ) , with 16.58 % crude protein and TDN 72.17% and was given 0.5kg /animal twice aday (at morning and afternoon)500 - 750 g / animal green fodder with wheat straw was given as it was available in the farm , cubic mineral and water provided freely at the day. Rams were training for semen collection for 30 days before the beginning of the study by using artificial vagina used for sheep. Semen was collected three time each month. The study includes two experiments : First experiment : It was designed to study the effect of breed , season ( spring , summer , autumn and winter ) and month on semen characteristics physical , sexual hormones ( testosterone , ICSH and SSH) of blood serum and level of enzymes ( AST , ALT and ALP ) of blood plasma.Second experiment : It included ten diluters from local materials (Beet pulp juice ) with 5 , 10 and 15% egg yolk and amino acids ( Glycin and cysteine ) with concentration of (5, 10 and 15) mmol. It also included the effect of type of dilutor , cooling , freezing storage period ( one and two months) in liquid nitrogen ( - 1960 C) on individual motility and live sperm percent .bThe results can be summarized as follow : 1 - Arabi rams exceeded (P<0.05) Najdi and crosses rams in their semen pH , spring also showed higher (P<0.05)pH followed by winter in comparison with summer and autumn .May showed higher (P<0.05) semen pH in comparison with other months.2 - Najdi rams exceeded (P<0.05)Arabi and their crosses in mean ejaculate volume, mass and individual sperm motility , sperm concentration , live sperm percent and mean testes circumference .3 - Summer and autumn showed higher (P<0.05) in meanejaculate volume , mass and individual sperm motility , sperm concentration , live sperm percent and mean scrotal circumference than winter and spring . Spring also showed higher (P<0.05) dead sperm percent followed by winter in comparison with summer and autumn .4 - August showed higher (P<0.05) ejaculate volume and mass and individual sperm motility followed July and June in comparison with other months. September , August and October exceeded (P<0.05)in comparison with other months sperm percent . October showed higher (P<0.05) in live sperm percent in comparison with other months . Highest (P<0.05) dead sperm percent happened during March followed by April and May . Scrotal circumference was high (P<0.05) during September followed by November and October.5 - Najdi and Arabi rams showed significant (P<0.05)differences in testosterone and ICSH hormones in comparison with their crosses . However , crosses exceeded (P<0.05)Arabi and Najdi rams in mean of SSH hormone. Autumn followed by summer exceeded (P<0.05) wintercand spring in testosterone , ICSH and SSH hormones. September and October showed higher (P<0.05)testosterone, ICSH and SSH hormones in comparison with other months.6 - Arabi rams exceed (P<0.05) both Najdi and their cross by enzymes concentration ( AST, ALT and ALP) of semen plasma . Spring and winter exceeded (P<0.05) summer and autumn by mean of ALT and AST enzymes. However , summer and winter exceeded (P<0.05) autumn and spring by mean of ALP concentration . March showed highest (P<0.05)AST and ALT concentration , while August showed highest (P<0.05) ALP concentration.7 - Amino acid diluter ( 15 mmol glysin and cysteine ) and 10 % beet pulp juice showed highest (P<0.05) individual motility after cooling and freezing for month and two monthsduring all months of year. While the beet pulp juice 15% and control showed the lowest means (P<0.05) of individual motility after cooling and two months of freezing.8 - Glysin 10 and 15 mmol , cysteine 10 mmol and beet juice 10% showed significant (P<0.05) increase in live sperm percent after and cooling and freezing . On other hand beet pulp juice 15% and control recorded the least value (P<0.05) . Beet pulp juice and control showed the highest (P<0.05) level of dead sperm percent after cooling and freezing in contrary with glysin 10 mmol and cysteine 15 mmol
