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التشكل الوراثي لجيني اللاكتوفرين والبرولاكتين وعلاقته بانتاج الحليب ومكوناته واداء الحملان قبل الفطام في النعاج العرابية == Relationship between Lactoferrin and Prolactin Genes Polymorphism and Milk Yield and its Components and Pre - weaning Lambs Performance of Arabi Ewes
Author name:
علي جاسم عبد الرضا
Supervisor name:
اسعد يحيى عايد | سمير اسطيفان حنا
General topic:
Agricultural sciences
Specific topic:
Zoology - Education
Degree:
Doctorate
University:
University Of Basrah - Faculty Of Agriculture
Language:
Arabic
University location:
Basrah
First pages:
31T1836 - p.pdf
Abstract:
Polymorphism analysis of lactoferrin and prolactin genes and its relationship with milk yield and its components and birth, weaning weight and growth rate before weaning were studied. DNA was extracted from 77 blood samples of Arabi ewes (21 ewes from Animal Farm, College of Agriculture, University of Basrah and 56 ewes from Animal Farm, College of Agriculture, University of Al - Muthana). Lactoferrin genotypes were determined by using Hind III restriction enzyme, those of prolactin by Hae III enzyme at Genetic Engineering Laboratory/ College of Agriculture/ University of Basrah and College of Animal Resources/ Mullah Thani University/ Islamic Republic of Iran. Milk yield was measured once a week from 52 ewes belong to Animal Farm/ College of Agriculture/ University of Al - Muthana. Samples were chemically analyzed. Birth and weaning weight were recorded as well as growth rate before weaning during a period of 1/2/2013 to 31/12/2013. Results revealed the following : 1 - The possibility of amplifying both genes and get two alleles for each and three genotypes of the lactoferrin gene (AA and AB and BB) and two genotypes (AA and AB) of the prolactin gene.2 - Prolactin and lactoferrin A allele frequencies were 0.94 and 0.66 respectively. AA genotypes frequency was 0.88 and 0.52; AB 0.12 and 0.29 and BB 0.00 and 0.19 of prolactin and lactoferrin genes respectively. population was at equilibrium for prolactin gene, but was not in lactoferrin gene.3 - Heterozygosity levels were 11.69% and 28.57% for prolactin and lactoferrin genes respectively. inbreeding coefficients (Fis) were - 0.05 and 0.345 for both genes respectively. University of Basrah and Al - Muthana herds showed very high genetic correlation (0.999).4 - The overall means of total and daily milk yields and lactation length were 61.13±2.76 kg, 0.582±0.04 kg and 104.75±1.95 days respectively. Ewe age and lamb sex did not significantly influence the above traits. However, ewes lambed during February exceeded thoselambed in September by daily milk yield (0.661 vs 0.577 kg) and lactation period (110.00 vs 100.00 days).5 - The overall means of total fat, protein and SNF and their percentages were 2.65 Kg and 4.33%, 2.5kg and 4.11% and 11.98% respectively. Only SNF was significantly influenced by ewes age and lamb age.6 - The overall means of birth and weaning weights and pre - weaning daily growth rate were 4.48±0.39 kg, 21.21±0.89 kg and 161.71±8.47 gm./day respectively. Lambs birth weight was significantly influenced by ewes age, ewes aged 4 and 5 years got heavier lambs than those aged 1 and 3 years.7 - Lactoferrin genotypes significantly affected protein and total solids not fat, as AA genotype exceeded the other genotypes. Fat of AA, AB and BB genotypes were 2.85, 2.23 and 2.63 kg respectively. while values of SNF were 7.24, 5.78 and 7.51 respectively.8 - There was arithmetic increase (not significant) in all traits for the AB genotype over AA genotype of prolactin gene.9 - Allele B of prolactin gene positively and significantly influenced most trait. It shared about 75% of each trait variation. That of lactoferrin gene shared about 25% only.10 - Heritability of SNF% and quantity was high (0.652 and 0.666 respectively). Those of birth weight and growth rate were medium (0.309 and 0.346). All other traits had low heritability.11 - Highest value of BLUP was recorded for daily milk yield, total milk yield and adjusted milk yield for 150 days (0.032, 3.072 and 2.220 kg respectively). The lowest values were - 0.021, - 2.071 and 0.00 kg respectively. Their ranges were 0.053, 5.143 and 2.220 kg respectively.12 - The lowest value of BLUP of fat%, protein% and SNF% were - 1.724, - 2.448 and - 4.565% respectively. the highest values were 2.058, 2.466 and 3.764% respectively with a range of 3.782, 4.914 and 8.329% respectively.13 - The lowest values of BLUP of birth and weaning weight and growth rate were 3.00 kg, 19.9 kg and - 1.20 g/day, the highest were 5.5 kg, 23.45 kg and 1.03 g/day with ranges 2.5 kg, 3.86 kg and 2.23 g/day respectively.14 - Breeding values of prolactin AB genotype of all studied traits exceeded those of AA genotype. However, those of lactoferrin AB and BB genotypes exceeded those of AA genotypes.
