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دراسة تاثير ضوء الليزر الثنائي الصمام (632 نانومتر) على بكتريا المكورات العنقودية الذهبية بوجـود المثلين الازرق كمتحسس ضوئي Study of Photodynamic Effect of (632 nm) Laser Diode Light On Staphylococcus Aureus Using Methylene Blue As A Photosensitizer

اسم المؤلف: ضياء خليل اسماعيل
الجامعة: جامعة بغداد
اسم المشرف: نورية عبد الحسين علي
الموضوع العام: علوم الحياة
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: بغداد
المستخلص: شملت الدراسة ثمان وسبعين عزلة من بكتريا المكورات العنقودية الذهبية عزلت من مئة وعشرين عينة تم الحصول عليها من عينات حروق وجروح قيحية وتجرثم الدم ومن مرضى يعانون من التهابات المجاري البولية, ومن اكثر من مستشفى للفترة من تشرين الثاني‏ / لغاية اذار/ 2014.تم | Seventy eight isolates of S. aureus bacteria were obtained from one hundred and twenty samples which were collected from different body sites and lesions (urine, blood and purulent wounds and burns) of patients from both sexes during the period November - 2013 to March - 2014. Methicilline sensitivity test (5µg MET disc) showed the appearance of methicillin - resistant in thirty seven Staphylococcus aureus isolates. Following exposure to laser light with a wavelength of 632 nanometer in the presence of Methylene blue at a concentration of 300µM at various exposure times (2, 4, 6, 8, 10, 12 and 15min), the results showed that the maximum decrease in viable colony counts ranging approximately from (6.9 to 3.8) log10 CFU /ml. Highly significant reduction in the viable count was achieved at 10, 12, 15 min exposure times, and 99% killing of cells were obtained when Photosensitisation of S. aureus using diode laser light at an energy density at 458.6 watt/cm2 for 15 mints. While in their exposure to the laser light in the absence of the dye or the dye in the absence of the laser light presented no significant effect on the viability of the S. aureus isolates. Both of phenotypic and genotypic investigation of the changes in virulence factors and the antibiotic - resistance were evaluated before and after irradiation with laser light.Results of photosensitization susceptibility tests showed large variations in the susceptibility, the isolates with resistant to methicillin before laser irradiation, become sensitive to it with percentage of 21.6%; in contrast the isolates with sensitive to vancomycine become resistant to it with percentage of 32.43%.On the other hand, the isolates that were resistant to Cefotaxime before laser irradiation become within the sensitivity range after laser irradiation with percentage of 51.35%, and also there were isolates within the sensitivity range before laser, become sensitive to Ciprofloxacin with percentage of 27.02% after irradiation. And the isolates of the S. aureus with resistant to Norfloxacin before laser irradiation, become sensitive to it with percentage of 16.2%. Also resulted in decrease the activity of ? - haemolysis, with 33 (90.3%) isolates of S. aureus in comparison to control as shown in blood agar method assay. In contrast had no effect on thermonuclease enzyme after irradiation.Detection of three genes represented in MRSA isolates by a confirmatory test was carried out using Polymerase chain reaction (PCR) technique. The results of the PCR amplification of mecA gene noted that it was present in 27 (72.2%) S. aureus isolates, While hlb gene detected 17(46%) isolates of 37 samples of S. aureus isolates, 14 of 17 hlb - positive S. aureus isolates(82.3%) were showed reduction in toxin production after exposure to laser light, whereas no altered or deficiency in thermonuclease gene (nuc).
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علاقه بعض طرز الجين كالبين - 10 مع حدوث مرض السكري من النوع الثاني في العراق Association of Some Calpain - 10 Gene Polymorphisms With The Incidence of Type 2 Diabetes Mellitus In Iraq

اسم المؤلف: مياسة مثنى خالد
الجامعة: جامعة بغداد
اسم المشرف: اسماعيل عبد الرضا عبد الحسن
الموضوع العام: علوم الحياة
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: بغداد
المستخلص: اجريت الدراسه الحاليه في معهد الهندسه الوراثيه والتقنيات الاحيائيه - جامعة بغداد خلال الفتره من كانون الثاني ولغاية حزيران - 2013 للكشف عن علاقة بعض الطرز الجين كالبين - 10 بحدوث مرض السكري من النوع الثاني في العراق. تم استخلاص الدنا من الدم الكلي باستخد | The present study was carried out in Genetic Engineering and biotechnology Institute - Baghdad University during a period from January to June, 2013, for detecting the association of some calpain - 10 gene polymorphisms with the incidence of type 2 diabetes mellitus in Iraq. Genomic DNA was isolated by using Geneaid DNA extraction kit from the whole blood; conventional PCR (SNP - 44 and Del/Ins - 19) and PCR - RFLP (SNP - 43 and SNP - 63) were used to detect the calpain10 variants by using specific primers and restriction enzymes. The study population consisted of 50 subjects with type 2 diabetes and 50 with normal fasting blood glucose (80 - 110 mg/dl). The type 2 diabetic subjects were recruited from the National Center for Diabetes treatment and Research. The non - diabetic control subjects were recruited from the same area as the comprising blood donors, healthy volunteers, or hospital /university staff members. Previous studies have detected a role for Calpain - 10 (CAPN10) polymorphisms in susceptibility to Type 2 diabetes mellitus (T2DM) in many populations. This study aimed to evaluate possible associations between these polymorphisms in the CAPN10 gene (SNP - 44, SNP - 43, Del/Ins - 19, and SNP - 63) and T2DM incidence in Iraqi population. Enrichment of allele 1(2R) in Del/Ins - 19 and 2R/2R genotype were found in T2DM patients. While the alleles and genotypes distribution of SNP - 44, SNP - 43 and SNP - 63 were not significantly different between patient groups and non - diabetic control subjects. The genotype AA in SNP - 43 and genotype TT in SNP - 63 were not found neither in T2DM nor in control subjects. of the eight haplotypes detected, enrichment of both haplotype 112 defined by variants of SNP - 43, Del/Ins - 19, and SNP - 63 and haplotype 2112 defined by variants of SNP - 44, SNP - 43, Del/Ins - 19, and SNP - 63 were seen in patients. The distribution of the other haplotypes was comparable between patients and control subjects. The calpain10 haplotype combinations were also obtained, and the haplotype combinations 111/111 and 111/112; which are created by variants of SNP - 43, del/ins - 19 and SNP - 63 and; haplotype combinations 1111/2111, 1111/2112 and 1121 / 2222; created by SNP - 44, SNP - 43, del/ins - 19 and SNP - 63; were associated with increasing the risk of T2DM.
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تقييم فعالية قاعدتين من قواعد شف الجديدة في بعض انسجة ذكور الفئران المختبرية البيض المصابة وغير المصابة بطفيلي المتحولة الحالة للنسج Entamoeba histolytica Evaluate The Effectiveness of Two Bases of The New Schiff Bases In Some Tissues of Male Laboratory Albino Mice Infected And Non - Infected With The Parasite Entamoeba Histolytica

اسم المؤلف: عبد الجليل عزيز كريم
اسم المشرف: فاضل عباس منشد العبادي حيدر عباس مهدي الحسيني
الموضوع العام: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
السنة: 2015
مكان الجامعة: ذي قار
المستخلص: تضمنت الدراسة الحالية تحضير وتشخيص قاعدتين من قواعد شف، حضر الليكاند الاول L1 من تفاعــل 2 - هيـدروكسي - 1 - نفـثالديهايد مــع الـسلفاميثوكسازول، وحضر الـثانـي L2 مـن تـفاعــل 4 - استميدوبنزالديهايد مع 4, 3 - داي امينو تلوين. شخص الليكاندان باستخدام ت | The present work includes a preparation and characterization of two schiff bases compound, the first from (L1) reaction of 2 - hydroxy - 1 - naphthaldehyde with sulphamethoxazole, and the second from (L2) reaction of 4 - Acetmidobenzaldehyde with 3, 4 - Diamino toluene. These compounds were characterized by the Infrared (IR) Spectroscopy, Nuclear Magnetic Resonance Spectroscopy (1H - NMR), Mass Spectra, and the results were virtually identical to what is expected. It has been determined LD50 for the two ligands - after dissolved dimethyl sulfoxide DMSO - and account for them safe therapeutic dose, reaching 0.14 g / kg and 0.24 g / kg, respectively. In order to see the effectiveness of the two ligands on the parasite compared with the infected group and treatment with a drug metronidazole, these two ligands have been administered orally the therapeutic dose each separately on two groups of mice which infected by the parasite Entamoeba histolytica. Three other groups of mice that non - infected have been also administered separately with the parasite first and second ligands and dimethyl sulfoxide, to see some of the side effects of these three compounds in comparison with the two groups of positive and negative control. At the level of probability (p ? 0.05), the results of statistical analysis show a significant variation, remarkable inhibitory effect of the number of cysts that reached the less impact to the second ligand rate about 2.9. in the seventh day of the injury and treatment. In the fifth day of the injury and treatment, the highest inhibiting effect of metronidazole was at a rate of 0, while in the seventh day of them, the first ligand was a compromise in effect, amounting to at a rate of 1.1. The Histological study of tissue of the cecum, liver and spleen showed a normal state for a negative control, and an occurrence of severe tissue changes in a positive control. In the cecum tissue, the mucosal was thin, and there were crashes in the crypts of Lieberkühn, with infiltration in inflammatory cells, as well as the expansion of the cells lining the crypts, and the disappearance of or crash submucosal layer, as well as severe hemorrhage in muscularis, and thickening in serosa. There was severe congestion of the blood vessel which was observed in liver tissue, as well as thrombus which appeared attached to the wall of the blood vessel, and infiltration of inflammatory cells out of the blood vessel toward the hepatic tissue, especially macrophages and eosinophils. It was also noticed the disappearance of hepatic architecture with degeneration of the liver cells and a clear sinusoids expansion. In addition to vacuolation of liver cells, that the tissue sections showed, with the occurrence of bleeding along the hepatic tissue. The changes in the spleen tissue represented in the occurrence of severe bleeding on the length of the lymphatic tissue of the spleen with infiltration of inflammatory cells, especially macrophages, and the happening of megakaryocytes. There is a remarkable improvement could be noticed in each tissues of cecum, liver and spleen of the infected groups and treatment with metronidazole and the first and second ligands. The improvement ratios were close to some extent : in the treatment group with metronidazole there was observation in tissue cecum to the occurrence of the four layers of its component, and the happening of a little bleeding which, with infiltration of inflammatory cells, and this is roughly similar to what shown by histological sections of two treatments by the first and second ligands. The liver tissue of the group treated with metronidazole showed the occurrence of radially arrangement obviously hepatic cells around the central vein, and liver cells with nuclei appeared clear and centralized with esinophilic cytoplasm, and hepatic cells proliferative as some binucleated hepatocytes, with a simple expansion to sinusoids and the proliferation of kupffer cells, the treatment groups with two ligands marked by the presence of a small clot within the central vein of the liver as well as reported in the treatment group with metronidazole. Tissues sections of the spleen tissue of infected mice treated group by metronidazole showed a clear proliferation of white pulp, which appears containing arterioles clear, white pulp is also surrounded by proliferation red pulp. And showed tissue sections of the group of infected animals and treatment by the first and second ligands as well as the proliferation of lymphocytes, with the presence of megakaryocytes.Showed the three groups non - infected by the first and second ligands and dimethyl sulfoxide, the lack of side effects on each of the cecum tissue, liver and spleen, except for a very simple infiltration of inflammatory cells from macrophages in the cecum of the group treatment by the second ligand
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دراسة فسلجية لمرضى الفشل الكلوي قبل وبعد الديلزة في محافظة واسط A Physiological Study of Renal Failure Patients Pre And Post Dialysis In Wasit Province

اسم المؤلف: تمارة حمد احمد العقبي
اسم المشرف: كاظم جهيد كاطع الطائي هيثم قاسم محمد
الموضوع العام: علوم الحياة
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2015
مكان الجامعة: واسط
المستخلص: تم اجراء هذه الدراسة في كلية العلوم/ قسم علوم الحياة في جامعة واسط وبالتعاون مع مركز الكلية الصناعية في محافظة واسط للفترة من 1/10/2012 ولغاية 1/5/ 2013 لغرض دراسة التغيرات الفسلجية التي تحدث لمرضى الفشل الكلوي بنوعيه الغسيل الدموي والغسيل البريتوني قبل و| This study was conducted in the Department of Biology, College of Science, University of Wasit. The samples were collected in cooperation with artificial kidney center in AL - Kut Hospital, AL - Kut city, Wasit Province, during the period from 1 /10/2012 to 1/5/2013. In order to study the physiological changes of renal failure patients before and after hemodialysis and peritoneal dialysis. The blood samples were taken from one hundered thirty four patients for this study.The patients in this study were divided into two groups according to the type of dialysis : 94 hemodialysis patients, 52 males with age range (21 - 69) years and 42 females with age range (20 - 70) years, 40 peritonial dialysis patients, 24 males with age range (21 - 60) years and 16 females with age range (23 - 63) years. All results were compared with the results of 57 healthy person who were selected from both sexes (29 males and 28 female). Eight milliliters of venous blood were collected from control subjects and patients with renal failure by using disposable syringe of 10 ml before and after dialysis process.The blood analyses was conducted for evaluating the hematological parameters which are included (Hb, PCV, WBCs and PLT ) biochemical parameters which include (Blood Urea, Serum Creatinine, Total Protein and Calicum) electrolyte parameters which include sodium and potassium, measured concentration of (Erythropoietin and Aldosterone) hormone in patients with renal failure before and after dialysis and control groups and measure the effectiveness of phagocytic cells in patients with renal failure before dialysis by using Nitroblue Tetrazolium stain (NBT). The study revealed the following results : 1 - When comparing the results of parameters between before and after hemodialysis and peritoneal dialysis with healthy control show the following : a - Significant decrease (P < 0.01) in the levels of (Hb and PCV) while significant increase (P < 0.01) in PLT in renal failure patients before and after dialysis compared to healthy control and significant increase (P < 0.01) in the numbers of WBCs in male peritoneal dialysis patients compared to healthy control.b - Significant increase (P < 0.01) in urea, creatinine, sodium, potassium and in the aldosterone hormone concentration in renal failure patients before and after dialysis compared to healthy control.c - Significant decrease (P < 0.01) in the erythropoietin hormone concentration, total protein, calcium and phagocytic cells activity in renal failure patients compared to healthy control.2 - When comparing the results of parameters between before and after dialysis for each type of dialysis showing the following : - a - All hematological parameters and the concentration of hormones (Erythropoietin and Aldosterone) observe no significant differences between before and after dialysis.b - Significant decrease in the levels of urea, creatinine and there is a significant increase in the levels of total protein and calcium after dialysis comparing with before dialysis.c - Significant decrease in the levels of potassium in hemodialysis patients after dialysis comparing to before dialysis and there is a significant decrease in the levels of sodium and potassium after peritoneal dialysis comparing to before dialysis. 3 - When comparing the results of males and females hemodialysis with males and females peritoneal dialysis shows the following : a - Significant increases in numbers of WBCs before and after dialysis in the male and female peritoneal dialysis comparing with hemodialysis b - Significant decreases in the levels of urea and creatinine after dialysis in the male and female peritoneal dialysis comparing with hemodialysis.c - No significant difference in the levels of Hb, PCV, PLT, total protein, calcium, sodium, potassium, the phagocytic activity and the concentration of hormones (Erythropoietin and Aldosterone) before and after dialysis in the male and female hemodialysis comparing with peritoneal dialysis. We conclude that renal failure patients in Wasit Province suffer from severe anemia, high blood pressure, increase in the levels of urea and creatinine, disturbance in the levels of potassium, sodium and calcium with deficiency in the body's immunity.
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دراسة التغايرات النسجية والمناعية لمرضى اللشمانيا الجلدية في محافظة ديالى Study The Histological And Immunological Variation of Cutaneous Leishmaniasis Patients In Diyala Province

اسم المؤلف: منى حميد احمد العزاوي
اسم المشرف: اسراء قاسم العبيدي
الموضوع العام: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
السنة: 2015
مكان الجامعة: ديالى
المستخلص: اجريت الدراسة الحالية على المرضى المصابين بداء اللشمانيا الجلدية والمراجعين لبعض المستشفيات والمراكز الصحية في محافظة ديالى للمدة من تشرين الاول 2013 ولغاية نيسان 2014. اذ بلغ عدد المصابين باللشمانيا الجلدية 115 مصابا وبمختلف المراحل العمرية يضاف لهم 25شخ | The present study was conducted on patients that infected with cutaneous leishmaniasis reviewing to some hospitals and health centers in Diyala province for the period from October 2013 to April 2014.The number of patients with cutaneous leishmaniasis was 115 in different ages in addition to them 25 person as a control group (uninfected). The proportion of infected women with cutaneous leishmaniasis ulcers was higher 56.522% than males 43.47%, the age group ten years and less was more group that being infected with rate 47.83% while the age group that rang from 71 to 80 was the less categories being infected in percentage 0.87% with a significant differences in the level of improbability less than 0.05. The presence of multiple ulcers was high 71.304% than single ulcers 28.696%. The presence of ulcers that length 1 cm and less (64%) more than other with more than 1 cm (36%) with statistically significant. The cutaneous ulcers were distributed on different areas of the body, the injuries on face was more 36.242% with anumber of 54 cases followed by upper limbs in rate 33.557% with anumber of 36 cases, the sore shoulder came latter with apercentage 1.342% in a significant differences at the level of improbability less than 0.05. The percentage incidence of cutaneous leishmaniasis was rise in kids and students in rats 33.043%, 39.130% respectively in a comparison with earners, housewives and of officers their percentages were 24.349% and 3.478% consecutively in statistically differences at 0.05 probability. The diagnosis of cutaneous leishmaniasis infections was done depending on clinical diagnosis based on the characteristics of the morphological and pathological ulcers, culture methods, impression smears and histopathological diagnosis. Clinical diagnosis for the cutaneous ulcer was recorded 100% percentage in comparison with culture method 10.435% versus 74.782%. percentage to positive smears, while the result of positive histopathological diagnosis was 88.571% on vision of promastigote of Leishmania parasite on semisolid medium while a adoption of existence amastigote from as appositive result of smears and histopathological method of the tissue biopsies taken from ulcer. The histopathological method was high efficiency supported clinical diagnosis when compared to other methods this rise in the diagnosis ratio has been demonstrated in significant differences in statistical probability less than 0.05.A study of amain impacts of histopathological changes in cutaneous ulcers on the infected skin layers at the level of light microscopy was also conducted. These pathological effects were in epidermis of skin including hyperkeratosis, pseudoepitheliomatous epidermal changes, ulcers and emergence of a dark colour, thickening in this layer accompanied suppurations and desquamation of chronic cases. As for the dermis layer, the inflammatory cells were infiltrate include plasma cells, macrophages, lymphocytes and eoisophils, disturbance in the order of layers especially in acute ulcers was taken, while the pathological effects were more in chronic ulcers as observed granulomatous inflammation and necrosis. The amastigote from were presence inside and outside the phagocytic cells.Aswell as some immunological changes that associated with the infection were studied in patients therapists by Pentostame (Sodium Stubogluconate) medication and uninfected control group by measuring the levelsof some cytokines IFN - ?, TNF - ?, IL - 12, IL - 17A and the chemokine MCP - 1. The level of IFN - ? was elevated in serum of cutaneous leishmaniasis patients and reached to 54.118±1.740 pg/ml, then the level was decreased during therapy and recorded 34.421±0.984 pg/ml after 1 - 3 dose a then dropped more after 4doses and more 22.489±0.795 pg/ml compared to uninfected control group 5.092±0.805 pg/ml. As well as the case of TNF - ? which rose their concentration in serum of CL patients and reached to 98.145±2.925 pg/ml, then decrease after treatment 71.507±2.901 pg /ml, 48.381±2.379 pg/ml after 1 - 3dose and more of treatment respectively, while the mean of TNF - ? in control group was 13.173±1.388 pg/ml.An increase in the level of IL - 12 was observed in the group of CL patients 845.598±16.273 pg/ml then the level was dropped during treatment and recorded 619.164±20.209 pg/ml, 495.578±27.038 pg/ml respectively but in control the concentration was 160.222±11.761 pg/ml. IL - 17A level was elevated in CL patients 227.268±5.027 pg/ml and decreased during therapy reach to 170.512±5.481, 126.443 ± 5.565 pg/ml after 1 - 3 doses and four doses and more respectively. The concentration of MCP - 1 chemokine was increased in CL patients 111.0397± 6.985 pg/ml then dropped during treatment 71.084± 2.636 pg/ml after 1 - 3 doses then dropped more 49.543± 2.167 pg /ml after four doses and more while they were in control 12.268 ± 1.609 pg/ml. The results of statistical analysis showed significant differences between these cytokines and their groups at the level of probability less than 0.05.
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عزل وتشخيص البكتريا العنقودية السالبة لانزيم الخثرة من حالات مرضية مختلفة ودراسة مقاومتها للمضادات الحيوية Isolation And Identification of Coagulase Negative Staphylococci From Different Clinical Cases And Study of Their Antibiotic Resistance

اسم المؤلف: مجاهد خلف علي احمد الجميلي
اسم المشرف: عباس علي هادي الدجيلي وعد محمود رؤوف
الموضوع العام: علوم الحياة
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: العربية
السنة: 2008
مكان الجامعة: صلاح الدين
المستخلص: جمعت 265 عينة من مستشفى تكريت التعليمي العام وشملت هذه العينات عينات سريرية لمرضى راقدين في المستشفى ومرضى مراجعين لها. اخذت العينات من المرضى المشتبه باصابتهم بخمج السبيل البولي وخمج الجروح والحروق وخمج الاذن الوسطى ولكلا الجنسين وباعمار مختلفة. انجزت هذ | A number of 265 samples had been collected from Tikrit Teaching Hospital (T.T.H.).These samples were taken from inpatients and outpatients suspected to have Urinary Tract Infection, Wound & Burn Infections and Mid - Ear infection for males and females in varied ages. The practical part had been fulfilled during the period from the beginning of December 2006 till the beginning of March 2007.Isolates diagnosed were identified depending on morphological and cultural characteristics. The results revealed 60 Staphylococci isolates, which shows 27 isolates (45%) were S. epidermidis, 13 isolates (21.66%) S. saprophyticus, 10 isolates (16.67%) S. xylosus, 3 isolates (5%) S. lentus, two isolates (3.33%) S. heamolyticus, and one isolate (1.67%) for each of S. simulans and S. hominis Ten (34.48%) of patients are located in the age group (15 - 30) years. while concerning the classification according to sex, females were (65.5%). Otitis media and Wounds and Burns infections, their ratio was high (61.1%, 53.8%) consequently both in males in comparison with females. Concerning the relationship between the infection and the residence and the level of education, the results revealed that isolation of Coagulase Negative Staphylococci (CoNS) were high in rural areas and in uneducated persons regarding the various infections than those live in urbans and educated ones. A of main tests were carried out in order to achieve the purpose of this study according to the planned goals, some of which are the virulence factors of Coagulase negative Staphylococci. These include DNase, Urease, Lipase, Lecithinase, and Alkaline phosphatase.The results revealed the ability of 67.5% of isolates to produce Alkaline phosphatase, and 46.6% of isolates to produce Urease, also 27.5% of isolates were able to produce Lecithinase , and the ability of 19.16% to produce Lipase, while 22.5% of isolates were capable of producing DNase. In addition to these tests, the test for producing ? - lactamase was done, 85% of isolates were positive to this test because ? - lactamase is one of the significant enzyme which are produced by bacteria since it improves the resistance of bacteria for antibiotics. Thirteen different antibiotics used for the Antibiotics susceptibility tests which show that most effective antibiotic is Nitrofurantoin (95%), followed by Amikacin (93.3%), Novobiocin(90%), Chloramphenicol(88.3%), Rifampicin (78.5%), Vancomycin (75%), Ciprofloxacin (73.3%), Clindamycin (70%), Gentamicin (58%), Penicillin G (50%), Trimethoprim (50%), Tetracycline (40%) and Cefoxitin (18.3%).
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تقييم خليط الكيتامين والترامادول والباراسيتامول والزايلازين كبرامج للتخدير العام بالحقن في الاغنام Evaluating of Ketamine, Tramadol, Paracetamol And Xylazine Combination As An Injectable General Anesthetic Protocols In Sheep

اسم المؤلف: علي اسماعيل جاسم
اسم المشرف: ثاير علوان عبد
الموضوع العام: الطب البيطري
الموضوع الدقيق: الجراحة
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: القادسية
المستخلص: هدفت الدراسة الى تقييم استخدام الكيتامين (K)، والترامادول (T)، والباراسيتامول (P) والزيلازين (X) كبرامج تخدير للاغنام باستخدام جرعات مختلفة وطرق اعطاء مختلفة. استخدم في التجربة ثلاثون من الاغنام الكبار البالغة من السلالة المحلية (تراوحت معدل اوزانها 27.4 | The study aim to evaluate the use of ketamine (K), tramadol (T), paracetamol (P) and xylazine (X) as an anesthetic protocol for anesthesia in sheep by using different doses and different routes of administration. Thirty animals of adult local breed sheep weighing (27.4±2.46) kg were used in 6 different anesthetic protocols. The animals were divided into six groups (5 animals of each). The first three groups (without using of xylazine) were giving the drugs by IV injection in the jugular vein : G1 giving K4 and P10 mg/kg IV, G2 giving K4T2 mg/kg IV, and G3 giving K4 T2 P10 IV. In the other groups (G4, G5, and G6) xylazine were adding to the anesthetic protocol. G4 giving K4 T2 P10 X0.05 by IV route. G5 was giving K10 T2 P10 X1 mg/kg by IM route and G6 giving K20 T4 P10 X2.5 mg/kg by IM route. The HR, RR, RT, muscle relaxation, limb, and flank analgesia, and rumen movement were taken before administration of anesthesia (time 0) and consider as the control reading. Then taken at 5, 10, 15, 20, 30, 45 and 60 minutes or till the end of anesthesia. In the IV groups (without using of xylazine), (G1, G2, and G3). The induction time was 53.2, 60, and 68 seconds, the surgical anesthesia was 2.8, 5, and 5 minutes, and the total recovery time was 4.8, 5.4, and 3 minutes in G1, G2 and G3 respectivily. The RR, and HR were increased, and the RT was decreased. The muscle relaxation was mild, and the analgesia was moderate. In G4 after adding of xylazine and giving the anesthetic protocol by IV route, the induction time, the surgical anesthesia, and the total recovery time were become 50.6 seconds, 23 minutes, and 9.4 minutes respectively. The HR, RR, and RT were increased, the muscle relaxation, and analgesia were moderate. In G5 after increased the dose of ketamine and xylazine and giving the anesthetic protocol by IM route, the induction time, the surgical anesthesia, and the total recovery time were 5, 36, and 5.6 minutes respectively. The HR and RR were decreased, and the RT was increased. The muscle relaxation was deep for 10 minutes, and the analgesia was moderate for 10 minutes also. In G6 after doubling the ketamine, tramadol, and xylazine doses, and giving the anesthetic protocol by IM route. The induction time, the surgical anesthesia, and the total recovery time were 3.6, 73.4, and 7 minutes respectively. The HR started stable, then after 15 minutes decreased sharply, The RR was irregular, with apnea, the RT was increased, the muscle relaxation was deep for 35 minutes, and the analgesia was deep for 45 minutes. In conclusion the addition of tramadol and paracetamol to the ketamine anesthesia of sheep in low doses, improve the induction and recovery time of anesthesia, without increase the duration and depth of anesthesia. Adding of xylazine to the anesthetic protocols increase the duration and depth of anesthesia. The protocol of K10 T2 P10 X1 by the IM route is the best protocol dose among the six protocols used in this study.
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تاثير فيتامين E والسلنيوم على الجرذان المحقونة بالذيفان الداخلي لجراثيم Salmonella typhimurium Effect of Vitamin E And Selenium On Rats That Injected With Salmonella Typhimurium Endotoxin

اسم المؤلف: زينة حمزة جعفر
اسم المشرف: جبار عباس احمد الساعدي محسن عبد نعمة الروضان
الموضوع العام: الطب البيطري
الموضوع الدقيق: الطب الباطني والوقائي
الدرجة: ماجستير
اللغة: العربية
السنة: 2010
مكان الجامعة: القادسية
المستخلص: صممت الدراسة الحالية بهدف تحديد التاثيرات الفسلجية والمناعية والنسجية المرضية التي تسببها الذيفان الداخلي لجرثومة Salmonella typhimurium وتداخل فيتامين E والسلنيوم في تقليل تاثيراتها السمية في اعضاء الجسم وتعزيز فعالية الجهاز المناعي لذكور الجرذان البيض. | The present study has been designed to determine the physiological, biochemical, immunological and histopathological effects of endotoxin of Salmonella typhimurium and the interaction of vitamin E and selenium in releifing its toxic effects on body organs, as well as its immuno - modulatory effect in mature male ratsForty mature male rats, nearly similar in their weight and age, were randomly divided into four equal groups : First group (control) rats were injected with normal physiological saline (0.5 cc ip) and 2nd group (T1) have been given the ration drinking water and injected with endotoxin intrapretonealy with dose 0.5 mg/kg bw for 18 days while (T2) and (T3) groups were treated by vitamin E with a dose of 30 mg/kg bw and selenium with a dose of 2.8 ppm/kg bw) for 30 days. (T2) was injected at thirty one day by endotoxin at a dose 0.5 mg/kg bw intrapretonealy for 8 days. Public health parameters like body weight, blood parameters (RBC count, PCV, total WBC count and differential leucocytes count, AST and ALT concentration) Phagocytosis activity for neutrophil and monocytes. Immunoglobulins (IgA, IgM and IgG) estimation, gross and histopath - ological changes and clinical sings were included in the present study.The results revealed that endotoxin injection led to different changes, its significancy flactuated according to the type of studied parameter, as follow : The results of clinical sings of experimental animals revealed occurrence of excitation after 10 minutes of injection, sever dullness, loss of appetite, muscular weakness, lateral recumbency in addition of loss of weight and easily handle. Significant decrease (P<0.05) of body weight, RBC count, ,PCV and monocytes percentage. Significant increase (P<0.05) of total WBC count and percentage of neutrophil, lymphocytes, eosinophil, and AST, ALT, IgM and IgA concentrations and no significant difference of IgG concentration. Significant decrease (P<0.05) of phagocytic activity.Gross pathological changes represented by the presence of necrotic and hemorrhagic foci, clotting as well as congestion and enlargement of liver, kidneys, lungs, spleen and heart. The histopathological appearance revealed by vaculation, necrotic and degenerative changes of hepatocytes in live, the spleen showed many changes led to imbalance between white and red bulb spaces, the heart showed lack of cross striation, edema between cardiac muscle fibers, while lungs showed an emphysema and sever hyperplasia in the bronchial epithelium. From other hand, the results showed that receiving vitamin E and selenium had an efficient effect for the improving the toxic effects of endotoxin and relieving its toxicological changes in the animal organs as compare with group that injected with endotoxin only, this group shows slight clinical signs and increase of appetite and drinking of water, improvement of blood parameters and reduce in the concentration of ALT and AST in blood serum, also there was increase in the phagocytic activity and increase in the levels of IgM, IgA and IgG. The gross pathological cganges less sever as compare with T1. It can be concluded that exposure to Salmonella typhimurium endotoxin leads to many distinct toxic effects on different organs and systems of the body which may results in many physiological disturbances. This study, also, proved that vitamin E and selenium have an important efficient role in improving the toxicological changes resulted from endotoxin poisoning.
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دراسة نسجية ,كيمو نسجية وكيمو حيوية لتاثير عقار الكودائين - باراسيتامول في ذكور الجرذان المختبرية Histological, Histochemical And Biochemical Study of The Effects of Codeine - Paracetamol In Male Sprague Dawley Rats

اسم المؤلف: اسيل كامل حميد الهاشمي
اسم المشرف: عادل جبار حسين صالح كاظم مجيد
الموضوع العام: الطب البيطري
الموضوع الدقيق: التشريح والانسجة والاجنة
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: البصرة
المستخلص: صممت هذه الدراسة لمعرفة التاثيرات النسجية, الكيمونسجية والكيموحيوية للاستخدام طويل الامد لعقار الكودائين - باراسيتامول على الجرذان المختبرية, حيث استخدمت في هذه الدراسة اربع وعشرون جرذ ذكر (وزن الحيوانات 200غم) قسمت عشوائيا وبالتساوي على اربع مجاميع. احتو | The present study is performed to evaluate the histological, histochemical and biochemical effects of long term use of codeine - paracetamol drug on laboratory rats, this study performed in college of veterinary medicine at university of Basra, there was (24) male rats(Body weight 200g) were divided randomly and equally into four groups, each group consisted of six rats for which codeine - paracetamol drug was orally administered for (90) days as the following : for group (A) normal saline was administered and it served as a control group. Group (B)was administered with (8/500 mg/200g) and served as low dose group, group (C)was administered with (16/1000 mg/200g) and served as intermediate dose group, and group (D) was administered with (32/2000 mg/200g) and served as high dose group. The statistical analysis results of body weight of the long term use of drug for the treated groups showed significant decreases (P?0.05) of body weight of both(L.D) and (H.D) while the (I.D) showed no significant (P?0.05) difference when compared to the control group. The internal organs weight (liver, kidney and spleen) showed significant decreases (P?0.05) of liver weights in both (L.D) and (I.D), while the (H.D) showed no significant difference of liver weight when compared to the control group; the kidney weight showed significant increases in both (L.D) and (I.D), while the (H.D) showed no significant difference when compared to the control group. Spleen weight also showed a significant increase in both (L.D) and (I.D), while the (H.D) showed significant decrease when compared to the control group. The serum lipid profile showed significant increases (P?0.05) in the levels of (cholesterol, triglyceride, High Density Lipo - protein and Low Density Lipo - protein) and no significant difference of (Very Low Density Lipo - protein) of (L.D), while the (I.D) showed significant increases of the levels of (cholesterol, HDL and LDL) and no significant differences of the levels of (triglyceride and VLDL), (H.D) also showed significant increases of levels of (cholesterol, triglyceride, HDL, LDL and VLDL) when compared to the control group. The statistical analysis of the results of liver and kidney function enzymes showed no significant differences of total serum bilirubin (TSB) of both (L.D) and (I.D) and a significant increase in (H.D); There was no significant (P?0.05) differences in the level of serum direct bilirubin (D.B) among all treated groups when compared to the control group , while the (H.D) group there was non significant compared with control and other treated groups, while the levels of (Aspartate Aminotransferase AST , Alanine Aminotransferase ALT, Alkaline phosphatase ALP and creatinine) showed significant increases in all treated groups when compared to the control group. Histological results of the liver of (L.D) and (I.D) showed vacuolation of hepatocytes and dilatation of sinusoids in which the vaculation is more severe in (I.D) with early septal fibrosis, while the liver of (H.D) showed severe centrolobular necrosis, hemorrhage, dilation of sinusoids and septal fibrosis. While the kidney of (L.D)and (I.D) showed vaculation of proximal convoluted tubules and glomerular mesengial cells with minimal vacuolation in (L.D), The kidney of (H.D) showed necrosis and vaculation of proximal convoluted tubules with vaculation of glomerular mesengial cells. The nervous system (brain, spinal cord and sciatic nerve) of all treated groups showed vacuolation of nerves cells and axons, severe vacuolation in (H.D). The lung showed edema and aggregation of inflammatory cells in lung of both (L.D) and (I.D) while the lung of (H.D) showed severe edema, enlarged proliferative and aggregation of lipid laden macrophages (pulmonary lipidosis). While the heart showed mild vacuolation of myocardial muscle cells of both (L.D) and (I.D), but the (H.D) showed sever vacuolation of myocardial muscle cells. The stomach also showed mild vacuolation of gastric epithelium in all treated groups. The testes also showed vacuolation and suppression of spermatogenesis in the (L.D) and moderate vacuolation and suppression of spermatogenesis in (I.D) while (H.D) showed present of giant multinucleated spermatid with sever vacuolation and suppression of spermatogenesis. While the spleen showed mild to moderate atrophy of white pulps of the (L.D) and (I.D), (H.D) also showed severe atrophy of white pulp with mild fibrosis and the presence of hemosiderin laden macrophages and foamy vacuolated macrophages. The histochemical study of poly saccharides(glycogen) of liver and kidney showed decrease of poly saccharides composition in mid zonal area and near the central vein, while in kidney, it was present in proximal convoluted tubules and in bowman's capsules in the (L.D) and (I.D), those changes were more severe in (H.D). While the histochemical study of lipid showed increase of lipid composition in hepatocytes near the central vein and in kidney in the proximal convoluted tubules and bowman's capsules, and those lipid compositions increased when the dose increased. The electron microscope study of liver showed mild to moderate swelling of mitochondria and dilation of smooth endoplasmic reticulum in both (L.D) and (I.D), while (H.D) showed severe swelling of mitochondria and dilatation with proliferation of smooth endoplasmic reticulum.
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دراسة تاثير بعض الاضافات على الاستجابة المناعية ووزن الجسم والتغيرات النسجية لفروج اللحم الملقح بلقاح مرض النيوكاسل A Study The Effect of Some Additives On Immune Response And Body Weight And Histological Changes For Broiler Chicks Vaccinated With Newcastle Disease Vaccine

اسم المؤلف: هبة تركي عطية الرماحي
اسم المشرف: نافع صبيح جاسم
الموضوع العام: الطب البيطري
الموضوع الدقيق: الامراض - الدواجن
الدرجة: ماجستير
اللغة: العربية
السنة: 2015
مكان الجامعة: القادسية
المستخلص: اجريت? هذه الدراسة لمعرفة تاثير بعض الاضافات في الاستجابة المناعية والوزن والتغيرات النسجية في الامعاء الدقيقة. حيث تم تقسيم (350) طيرا من فروج اللحم بعمر يوم واحد من نوع Ross 308) ) غير مجنسة , وزعت عشوائيا الى (7) معاملات تغذوية متساوية وهذه المعامل | This study was conducted to investigate the effects of feed additives on immune response , weight and histological changes in small intestine. A total of (350) broiler chicks one - day old (Ross 380) - unsexed were randomly divided in to (7) equal dietary treatment groups , as fallows : Treatment (T1) : Basal diet without any addition ( control group).Treatment (T2) : Basal diet + Garlic 4cc/10 LTreatment (T3) : Basal diet + Saccharomyces cervisiae 2.5gm/10 L.Treatment (T4) : Basal diet +Stpire 2.5cc/10 L.Treatment (T5) : Basal diet +B. glucan 8gm/10 kgTreatment (T6) : Basal diet + Levamisole 1gm/kg.Treatment (T7) : Basal diet + Neomycin 0.5 gm/L.These additions were presented on the first day of the arrival for 5 days, and on the day of first vaccination for 5 days and at the end of experiment for 5 days also. This chicks vaccinated with NDV on the day one of age by injection in the neck and re - vaccinated at day 10 of age with LaSota, Body weight were weekly determined. ,blood samples were collected from wing vein for ELISA test in ( 4,18,27 ) days old respectively. The results as following : - 1 - Treatment of levamisole (T6) were significantly increased ( p<0.05) in (T2, T5) in level of Abs production against ND virus.2 - Chicks feed diet with saccharomyces cerevisiae showed significantly increased ( p<0.05) in body weight among all experimental group , and improvement in weight in (T6,T7) with other group.3 - Chicks feed diet with (T7) showed improvement in villus hight and in the ( T3, T4).
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دراسة التاثير العلاجي لحليب الافراس المخمر للتقليل من تاثير الاجهاد التاكسدي المستحدث بواسطة خلات الرصاص في الجرذان البيض Study The Therapeutic Effect of Fermented Mare? S Milk To Reduce The Effect of Oxidative Stress Induced By Lead Acetate In White Albino Rats

اسم المؤلف: شيماء عباس صبيح
اسم المشرف: كريم ناصر طاهر
الموضوع العام: الطب البيطري
الموضوع الدقيق: الصحة العامة
الدرجة: ماجستير
اللغة: العربية
السنة: 2015
مكان الجامعة: القادسية
المستخلص: اجريت الدراسة الحالية في البيت الحيواني التابع لكلية الطب البيطري / جامعة القادسية بهدف تقييم الدور العلاجي لحليب الافراس المخمر في التقليل من تاثير الاجهاد التاكسدي المستحدث بواسطة خلات الرصاص. استخدم في هذه الدراسة ( 40 ) ذكر من الجرذان البيضاء نوع ( w | The present study was conducted at the animal house of veterinary medicine college of AL - Qadisiyah University during the period from 27 - 2 - 2014 to 30 - 3 - 2014. to evaluate the productive role of fermented mare's milk in to reduce the oxidative stress that induced by lead acetate , 40 male albino rats weighing between 200 - 210 gm were used in this study. the animals were obtained from the animal house of veterinary medicine College / Baghdad University. The animals were randomly distributed into four equal groups , ( 10 rats per each group / cage) as follow : - Control group : The rats were given only basal diet and deionized drinking water - - First treatment group ( T1) : The rats were given basal diet and deionized drinking water that contain 1000 ppm lead acetate for 30 days. - Second treatment group ( T2 ) : The rats were administrated basal diet and deionized drinking water that contained 1000 ppm of lead acetate and received orally ( by gavag ) fermented mare's milk at a dose ( 10ml / kg b.w / day ) for 30 days. - Third treatment group (T3) : The rats were given basal diet and deionized drinking water that contained 1000 ppm of lead acetate and received orally ( by gavag ) EDTA at a dose ( 50 mg/ kg b.w / day ) for 30 days. At the end of the experiment all the animals were fasted for ( 12 hours ) and then killed animals from each treatment group blood samples were collected from the heart to measures some blood parameters such RBC , Hb and PCV and biochemical parameters such as liver function parameters ( total protein and activity of some enzymes such as ALT , AST , ALP and LDH ) , kidney function parameters such as Urea and Creatinine concentration and oxidative stress parameters such as GSH , SOD , MDA ). In addition to that study the histopathological changes of liver and kidney tissues were studied The results of the present study can be summarized as follow : - 1 - Blood parameters : The results of the statistical analysis showed that there was a significant ( p < 0.05 ) decrease in the mean values of RBC , Hb , and PCV in a group treated with lead acetate only ( T1 ) as compared to other experimental groups. while groups treated with EDTA and fermented mares milk showed a significant ( p < 0.05 ) improvement in blood parameters. 2 - Biochemical parameters : A - Liver function parameters : The result revealed that there was a significant ( p < 0.05 )effect of lead acetate on level of total protein where caused a decrease in total protein concentration and increase the in activity of ALT , AST , ALP , and LDH as compared to other experimental groups. Yet treatment with fermented mare's milk ( T2 ) resulted in an increase in the total protein concentration and a decrease in the activity ALT , AST , ALP AND LDH. Also treatment with EDTA ( T3 ) caused a similar results to ( T2 ). B - Kidney function parameters : The results obtained in this study demonstrated that a significant ( p < 0.05 ) increase in the level of urea and creatinine in a group of animals treated with lead acetate only ( T1) , while using fermented mare's milk ( T2 ) and also EDTA contributed reduction in the level of urea and creatinine 3 - Oxidative stress parameters : The results of the present study showed that there was a significant ( p < 0.05) decrease in the concentration of GSH and SOD a significant ( p < 0.05 ) increase in MDA concentration in animals that administrated lead acetate only ( T1) while administration fermented mare's milk and EDTA resulted in significant ( p < 0.05 ) increase in the concentration of GSH and SOD while a significant ( p < 0.05 ) reduction in MDA concentration. The values becomes close to the concentration of the control group. 4 - Histopathological changes : The results of Histopathological examination for tissues of liver and kidney of rats given lead acetate alone ( T1 ) showed that there was a clear Histopathological changes presented in degeneration of hepatocytes and congestion in central vein which associated with hemorrhage in liver and kidney tissues of animals administrated fermented mare's milk showed normal and free from congestion.
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عزل وتشخيص النمط المصلي O78 : K80 لاشريشيا القولون الممرضة للطيور من دجاج اللحم ودراسة اصابتها تجريبيا Isolation And Identification of Avian Pathogenic E. Coli O78 : K80 Serotype From Broilers And Study of Its Experemintal Infection

اسم المؤلف: رجاء عبد الزهرة علي
اسم المشرف: علي عبد سهم المياح
الموضوع العام: الطب البيطري
الموضوع الدقيق: الامراض - الدواجن
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: البصرة
المستخلص: الايشريشيات القولونية مسئولة عن احداث حالات مرضية مختلفة تسبب خسائر اقتصادية ناتجة عن الهلاكات العالية وانخفاض معدل التحويل الغذائي والخسائر في اتلاف الجثث والاعضاء المصابة بعد الذبح.تم في الدراسة الحالية فحص 70عينة من افراخ فروج اللحم الهالكة او التي كا | Escherichia coli (E. coli) is responsible for a variety of diseases conditions which cause high economic losses in poultry due to high mortalities, decreased food conservation rate and condemnation of whole affected carcass or organs after slaughter. In the present study, 50 out of 70 broiler chickens which have been collected from diseased chickens were exhibited lesions of fibrinous perihepatitis, fibrinous pericarditis and/or airsacculitis. Birds with these lesions were subjected for bacteriological examination. The examination revealed isolation of 23 E. coli isolates with incidence of 46%. Concerning the virulence factors , Congo red binding activity of these isolates reveled detection of 6 positive isolates with incidence of 26.08%. Serotyping showed that out of 6 Congo red positive strains, only 3 strains were serologically typed and were belonged to the serotype O78 : K80. Detection of pathogenic E. coli serotypes was confirmed by PCR technique with specific primers for fimA and fimH genes. All these 3 isolates of the serotype O78 : K80 were reacted with these 2 genes. Antimicrobial suscebtibility pattern displayed sensitivity of these isolates to Chloramphinicol and Gentamicin and their resistance to Amoxicillin, Erythromycin and Nalidixic acid. Experimental infection has been made with this isolate. Two groups of broiler chickens were used the first at one day old and the second at 5 weeks of age. Signs, lesions, histopathological changes were revealed that the first experiment was differed from the second through the presence of pus in the bursa of Fabricius, but hemorrhage in the lungs tissue occur in two experiments.
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دراسة الاهمية الوقائية لحليب الماعز وتاثيراته في بعض الصفات الانتاجية والمناعية لاناث الارانب المعاملة بالمضاد الحيوي اموكسي كلافولانيك اسيد The Important Protective Study of Goat Milk And Its Effects On Some Productive And Immune Traits of Female Rabbits Treated With Amoxi - Clavulanic Acid

اسم المؤلف: حسين باعي خضر حسين الجياشي
اسم المشرف: كريم ناصر طاهر علاوي لعيبي داغر
الموضوع العام: الطب البيطري
الموضوع الدقيق: الصحة العامة
الدرجة: ماجستير
اللغة: العربية
السنة: 2012
مكان الجامعة: القادسية
المستخلص: This study was carried out from 12 - 3 - 2011 to 29 - 4 - 2011 to find out the protective importance of goat milk and its effects on some productive traits ( body weight , weight gain , feed intake, and feed conversion efficiency) ,some immunological traits ( white blood cells , percent of lymphocyte cells) ,some biochemical traits (total serum protein , albumin ,globulin ) ,some physiological traits ( hemoglobin and Packed cell volume) in the rabbits treated with Amoxicillin - clavulanic acid.A total of (32) female rabbits (local breed) aged range between 2 - 3 months with average of initial body weight 1706.25 gm.were distributed randomly into four groups each one consist of eight rabbits as below : 1 - Control group ( C ) : Fed basal diet without any addition.2 - First treatment group ( T1 ) : Fed the same basal diet + 5 mg ? kg b.w. x 2 amoxi - clavulanic acid for one week3 - Second treatment group ( T2) : Fed the same basal diet +( 5 mg ? kg b.w. x 2 amoxi - clavulanic acid + 5ml x2 raw goat milk ) for one week.4 - Third treatment group ( T3 ) : Fed the same basal diet + 5ml x2 raw goat milk for one week.The experiment continued for five weeks in which each rabbit weight at weekly interval and the residual feed weighed daily ,The results revealed the following : - Treatment one showed signs of gastric ulcer ,diarrhea , small and large intestinal inflammation ,while T2 group ( antibiotic + goat milk ) did not show any signs of gastric ulcer, diarrhea and their digestive system normal like the control ( C ) and Treatment three ( T3 ) group ( goat milk ).Body weight of ( T3 ) group recorded significantly) P < 0.05 )increased as compared with body weight of T1 group in all study weeks. And recorded the highest body weight among the other study groups during all periods of the study weeks (1769.12, 1751.37,1795.00 , 1824.25 ,1852.75) gm respectively.Animals in group ( T3 ) exceeded in weekly body weight gain as compared with other treatment groups during the studied period except the second week ( 62.87, - 5.25, 32.5, 29.25 , 28.5) gm respectively.Treatment ( 1 ) showed decrease in weekly feed intake on the first 4 weeks of study ) 60 , 42.51 , 44.88 , 34.25 gm )respectively as compared with C and T3 groups.Treatment ( 3 ) showed that feed conversion efficiency means recorded better value during the fifth weeks of studied weeks and was ( 5.53 , - 64.30 , 10.79 , 11.98, 12.11) gm food ? gm gain respectively recorded.There are significant increases ) P < 0.05 ) in white blood cell means for C ( control ), T2 , T3 groups) 6.352, , 6.32 6.45) x 10 3 ? ?L. compare with the T1 group ( 4.772 x 10 3 ? ?L ) and also lymphocyte percentage means of T1 (38.95 % ) showed significant lower ) P < 0.05 ) compared with other treatment groups ( C , T2, T3 ) ( 55.05 , 54.80 , 57.07 ) respectively.The control and T3 groups showed significant) P < 0.05 ) exceeded in hemoglobin and packed cells volume as compared with T1 and T2 groups which there is no significant difference between them.Where the average of four treatment group control ,T1 ,T2, T3 record 44.837 , 38.675 , 38.825 , 44.800 respectively.Treatment 3 exceeded other treatment groups in the total protein ( 88.156 )gm/l and also T1 group showed significant ) P < 0.05 ) decrease in albumin ratio ( 26.682 ) gm/l, while T1 showed significantly ) P < 0.05 ) higher in their gamma globulin compared with other treatment groups.Treatment 3 and 1 groups showed significant ) P < 0.05 ) increase in liver relative weight as compared with the control and T2 groups and the liver relative weight means of the ( C ,T1 ,T2 ,T3 ) groups as followed respectively 2.64, 3.05, 2.53 , 3.08 %.
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The Role of Cyclosporine In Intra Bone - Bone Marrow Transplantation In Male Rabbits

اسم المؤلف: عادل جاسب عويد
اسم المشرف: Abdulbari A. Alfaris Alaa A. AL Sawad
الموضوع العام: الطب البيطري
الموضوع الدقيق: الجراحة
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: البصرة
المستخلص: استخدم فيها 30 ارنبا ذكرا حيث قسمت الى ثلاثة مجاميع متساوية بالعدد كل مجوعه تحوي على عشرة ارانب اثنان منها تم اختيارها كواهب لنخاع العظم والثمانيه الاخرى كانت مستلمه, وكانت المجموعه الاولى هي مجموعه سيطرة وقد اعطيت المحلول الملحي الطبيعي ,والمجموعه الث | Thirty male rabbits were randomly divided into three groups as following : Group one (sham group) consists of ten male rabbits, two of them were used as donors and other eight were administered 12.5ml/kg/BW normal saline (N.S) orally for ten days. At Zero time of the experimental the collection of blood samples (10ml) was carried out on each animal from the heart (cardiac puncture) and by a sterile syringe 22 Gage needle. The (10ml) amount of blood was divided into two parts. (5ml) was deposited into tubes without anticoagulant and then refrigerated for a maximum of 12h and centrifuged at (5000 rpm) for 15 minutes these serum samples were stored in polyethylene eppendorff tubes at - 20?C Estimation of Total interleukin 2 in serum by using an Enzyme - Linked Immunosorbent Assay (ELISA). The 2nd sample was also (5ml) taken for performing hematological test to measure the WBCs account and their differential, RBCs account, Hb & P.C.V. At Day10 of experimental was collecting the blood as similar procedure at zero time and did a similar examination before operation. At Day11 of the experimental an allogeneic Intra - bone - marrow injection of bone marrow transplantation aspiration method (IBM - BMT) AM was carried out and rabbits were re administered with normal saline after surgical operation until Day21 of experimental. Following that rabbits were anesthetized with Ketamine (20 mg/Kg/BW) and xylzine (9 mg/Kg/BW) intra muscular. Before bone aspiration for bone marrow histological test the smears were stained with Giemsa stain. At the end of this experimental study of there was collection of blood as a same procedure at zero time and DAY10 then made the same test to estimate of blood values and interleukin 2. Then all recipients’ rabbits were sacrificed and bled to death to obtain the Kidney and liver, which were dissected out, trimmed and processed for the histological study with used Heamatoxyline and Eosin Stain. Group two (treated with 12.5ml/kg/BW cyclosporine orally administrated) and same protocol of the sham group was applied except when using (treatment with 12.5ml/kg/bw cyclosporine orally administrated) replacing the normal saline. Group three (treatment with 25ml/kg/BW cyclosporine orally administrated) and same protocol of sham group was applied except when using (treated with 25ml/kg/BW cyclosporine orally administrated) replacing the normal saline. The results of the present study Conclusions cyclosporine effects on the bone marrow, immune system because of decrease of WBCs, lymphocytes, neutrophils and interleukin 2.The study Conclusions cyclosporine caused many adverse effects, where by it caused severe liver and kidney; therefore it was considered predisposing factor to infection with several diseases as well as it is considered the main cause of disorders in renal function renal dysfunction. The results of the present study Conclusion the surgical operation of intra - bone - bone marrow transplantation more safe than other ways of intra vinous bone marrow transplantation also the study the operation increase the hematopoietic cells
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دراسة تاثير الفصول على بعض الصفات التشريحية والفسلجية للجهاز التناسلي في ذكور الجمال وحيدة السنام (Camelus Dromedarius) Study of The Seasonal Effect On The Some Anatomical And Physiological Features of Parts of The Genital System In Adult Male One Humped Camel (Camelus Dromedarius)

اسم المؤلف: عماد حمزة مروح
اسم المشرف: ضياء حسين جاسم الدليمي
الموضوع العام: الطب البيطري
الدرجة: ماجستير
اللغة: العربية
السنة: 2013
مكان الجامعة: القادسية
المستخلص: اجريت الدراسة الحالية على (96 عينة) لاجهزة تناسلية (خصى وبرابخ )ودم لذكور جمال ناضجة جنسيا بمعدل (6 - 5سنة) بعد التاكد من سلامتها قبل الذبح وبعده والتاكد من عمرها بواسطة المعادلة السنية من مجازر (النجف وعفك والديوانية ) لمدة سنة كاملة بواقع (8عينة) لكل شه | The current study was conducted on(96 samples) of genital organs (testes and epididymis) as well as blood of male mature camels (5 - 6 years) after the confirmation of their health before and after slaughter, and to make sure from their ages by the dential equation,these sample were collected from abbatoris of AL - Najaf, Afak and Diwaniya) for the full year with (8 samples) per - month for the purpose of determination parameters anatomical parameters for some of the genital system of the testes and epididymis (right and left), physiological parameter (hormonal elements chemical), the study anatomical results biggest weight, length, width and circumference of the lift testis and the weight and length of the left epididymis , mis recorded in winter significally(p <0.05), which reached(90.041 ± 0.613, 103.904 ± 0.633, 50.541 ± 1.957, 256.812 ± 0.615, 40.500 ± 0.288, 109±0.7), respectively, while less weight, length, width, circumference, weight and length of of the testis left and weight length of lift epididymis which recorded in the summer significally (p <0.05)and reached (85.666 ± 0.441, 92 ± 0.357,, 40.583 ± 0.220, 242.266 ± 0.15, 35.083 ± 0.083, 94.34 ± 0.45) respectively, while the measurements were in the weight, length, width and circumference of the right testis and weight and length of the right epididymis was recorded in winter and the level of significance (p <0.05), recorded(89.373 ± 0.474, 101.958 ± 0.622, 48.895 ± 1.181, 256.060 ± o.688, 35.945 ± 2.878,30.728 ± 0.518) respectively, , while the measurements of the less weight, length, width and circumference of the right testis and weight and length of the right epididymis was recorded in the summer in level of significance (p <0.05)and recorded (84.700±0.472,97.314±0.291,39.666±0.707,241.104±0.104,26.395±0.055, 92.5±0.3) respectively, the results of physiological and features related with hormonal the measurements that Folicul stimulate hormone (FSH) and lutyal hormone(LH) and (Testosterone hormone) have recorded the highest concentration in the winter and the level of significance( p<0.05) and recorded (5.128 ± 0.455, 2.770 ± 0.3184, 10 ± 1), respectively, while the lowest levels were recorded in summer with level of significance( p<0.05) and recorded(1.633 ±0.130 IU,2.145 ± 0.13 IU, 3.5 ± 0.091 ng/ml) while prolactin hormone has recorded lowest level of significance (p <0.05) in the winter and the highest level in the summer which recorded (102.2 ± 8.405 IU ,244 ± 24.463 IU)respect ally. the chemical elements increased their ranges in the winter and spring (p <0.05) which reached each of calcium, magnesium, sodium and potassium (8.791 ± 0.122 mg/dl, 3.016 ± 0.130 mg/dl, 147.916±2.061 Mmol/L, 7.000±0.003Mmol/L) and in the spring(8.100±0.52mg/dl,2.279±0.056mg/dl,146.458±3.129Mmol/L,8.405±5.181Mmol/L).while the lowest levels were in summer(p<0.05)which recorded(7.683 ±0.202, 2.236 ±0.148mg/dl,145.454 ±0.275Mmol/L,24.463 ± 5.027Mmol/L) respectively
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عزل وتشخيص جزيئي لمورثات مجاميع الانماط المصلية لجرثومة اللستيريا المستوحدة Listeria monocytogenes من الانسان والحيوانات في محافظة القادسية Isolation And Molecular Detection of Serotype Genes Group of Listeria Monocytogenes From Human And Animals In Al - Qadissyia Province

اسم المؤلف: حسين عمران كريم العابدي
اسم المشرف: هدى عبد الهادي علي النصراوي
الموضوع العام: الطب البيطري
الموضوع الدقيق: الامراض المشتركة
الدرجة: ماجستير
اللغة: العربية
السنة: 2015
مكان الجامعة: القادسية
المستخلص: اجريت الدراسة الحالية خلال الفترة من تشرين الثاني 2014 الى نيسان 2015، جمعت العينات المرضية من النساء التي تعاني من حالات الاجهاض وولادة مبكرة والاطفال المصابين بالتهاب السحايا من مستشفى الولادة والاطفال التعليمي وقد جمعت العينات تحت اشراف طبي متخصص وكان | This study was carried out during the period from November 2014 to April 2015, whereas samples were collected from women that suffered of abortion and infected children in the maternal and children hospital in Al - Qadissiya province , so the samples were collected under supervising of a specialist medicine , the number of taken samples of the women is 65 samples , while the number of taken samples of children is 32 samples , so the samples that belong to animals have included a gall bladder samples were collected from Al - Diwaniya slaughterhouse were 100 samples of sheep and 100 samples of cows. in addition to collect 200 samples of milk as 100 samples for each sheep and cows from different rural zones in al - Qadissiya province. L.monocytogenes was isolated by using oxford listeria selective agar for listeria , so the special stimulator for growth of the bacteria was added to it in order to determine the type of bacteria , also it diagnosed an isolates for L.monocytogenes by using the microscopically examination and biochemical tests , furthermore the germ isolates was planted on blood agar for diagnosing the blood analysis type : ? - heamolysis as affirmative checking for the isolates. also PCR technique used for affirming the diagnosing of L. monocytogenes by using the initiator for the blood analysis gene (hly) , whereas all the L. monocytogenes isolates' gave a positive result in inspection , so this technique is used too in the detection about the serous types for L.monocytogenes isolates by the molecular diagnosing for the fierce genes of the germ and these are (D1,D2,Glt,FlaA and MAMA gene ). The results of the research showed that isolating and diagnosing percentage of the Listeria monocytogenes is about 9.27%, whereas the isolation percentage in women infected in abortion is 4.61%, while the percentage of listeria germ isolation was higher in the children that infected in meningitis whereas it was 18.75% of the total amount of the samples.As for the percentage of the isolating in sheep it was 4% and 7% for milk and gall bladder respectively. In the cows the isolation percentage of the germ from milk and gall bladder was 2% and 3% respectively. So the the results of isolating for listeria germ during the study period in human and animals were disparate , whereas the highest percentage of isolation for the germ in human were scored in January and February months of the year whereas it scored 20% and 16% respectively.As for sheep the percentage of isolation was higher in December and January months whereas it is 6.25% and 10% respectively , while in the cows the percentage was higher in January and February months where it is 4.2% and 7.4% respectively. Regarding The results of detection about the genes of the serous types for the isolates of L.monocytogenes by using PCR technique 14 isolates of the samples showed a positive result when they react with the initiator for gene (D1)in percentage about 56%, so this isolates showed a positive result when they react with the initiator for gene (Glt) as amount of 5 isolates and they classified to be belong to the serous types (1/2B or 3B), while 9 isolates gave a negative result in the inspection , whereas these isolates were tested with the initiator for gene (MAMA), 5 isolates showed a positive result in this reaction with this gene and they classified to be belong to the serous types (4c or 4a), while 4 isolates gave a negative result as they classified to be belong to the serous types (4b or 4d or 4c).As for the isolates' that gave a negative result with the initiator of gene (D1)there are 11 isolates all these isolates gave a positive result when they inspected with the initiator of gene (D2) in a percentage about 44%, so when these isolates inspected with the initiator for gene (FLaA) then 6 isolates of them gave a positive result in the inspection and classified as they belong to (1/2a or 3a), while 5 isolates of them gave a negative result in the inspection and classified as they belong to (1/2c or 3c). As for the serous types that have not diagnosed in the germ isolates of the human there are(1/2a or 3c), while in the germ isolates of the sheep the serous types (4a or 4c) have not been diagnosed , then the serous types (1/2b or 3b) have not been scored in the cows. The present study concluded that the percentage of infection in L.monocytogenes was high in human in al - Qadissiya province , in which that refer to the risks of the diseases and health problems spread that could be caused by L.monocytogenes , also the results included isolating the germ from milk and gall bladder samples for sheep and cows that could be a source to carry the infection to the human by contaminating the meat, milk and its derivatives'. whereas the isolation percentage of the germ was higher in the cold months of the year in human and animals, so the highest isolation percentage of the serous types for the L.monocytogenes in human and cows were (4a , 4c) , while in sheep the serous types were (1/3c,3c).
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الامراضية السمية لنابروكسين الصوديوم في الجرذان البيضاء مع الكيتوبروفين كعامل تحكم ايجابي Toxicological Pathology of Naproxen Sodium In White Laboratory Sprague Dawley Rats (Rattus Rattus) With Ketoprofen As Positive Control

اسم المؤلف: ثائر علي محسن
اسم المشرف: زينب وحيد خضير صالح كاظم مجيد
الموضوع العام: الطب البيطري
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: البصرة
المستخلص: ان الغرض من الدراسة هو تحديد السمية المرضية الدوائية لنابروكسين الصوديوم (كعامل مضاد التهاب غير استيرويدي) في الجرذان البيضاء، مع الكيتوبروفين كعامل تحكم ايجابي. لانها ذات فائدة على نطاق واسع في الحيوان والانسان، لتلقي العلاج الميداني، ومعرفة العلامات الس | The purposes of study are : first, to determine the toxicity and pathogenesis of naproxen sodium [as non - steroidal anti - inflammatory agent(NSAIDs)] in white rats in comparison with ketoprofen as positive control, as they are of wide use in animal and human, for treatment of variable disease; and knowledge of the clinical signs, macroscopic changes and microscopic changes by toxic dose of naproxen, second to observe note - 1 the histological changes in comparison with the control group (as untreated group), and note - 2 to examine the biochemical parameters in response to naproxen treatment, note - 3 where naproxen is (NSAID) with analgesic and antipyretic properties. In the present study, white rats are used. Divided into five groups each group contain 12 rats; these group divided into the following manner : The first group - C, representing untreated group has been treated with normal saline only; the second group - L, representing the low dose which has received (5mg/kg B.W) of naproxen sodium, while the third group - I is the intermediate dose, which has received (10mg/kg B.W); the fourth group - H, representing the high dose, that has received (20mg/kg B.W), and finally the fifth group, representing the positive control group, has received(4mg/kg B.W of ketoprofen). The method of dosing these animals are by oral gavage which continues for three months. All the animal groups have been put in the same conditions of temperature and humidity. This study shows that the treatment with naproxen sodium has led to significant gradually increase in the body weight of both high dose and intermediate dose groups in early treatment period in comparison with the control group. In contrast, the animals which have received low dose of naproxen sodium show only minimal and gradual increase in their body weight in comparison with the intermediate and high dose. As well as there has been noticeable little increase and decrease in some value of liver and kidney enzymes concentrations(AST, ALT, ALK, Urea/Cr as treated with naproxen and positive control of ketoprofen). The study also showed that the treatment with naproxen sodium had led to clinical findings include uterine hemorrhage and still birth which specially occurs in the last period of pregnancy. Infections happen in some regions of body forming abscess in the subcutaneous tissue of neck, leg, cheek. The macroscopic findings include pallor of liver and Abscess of the liver and kidney, also there is increase in size of the spleen as a result of congestion of the splenic red pulp and minimal changes of the mucosa of the stomach. Moreover the microscopic findings include minimal hepatic periportal fibrosis , moderate diffuse vacuolation of hepatocytes, area of vacuolated degenerative centrilobular hepatocyte arround the central vein and subcapsular infiltration of inflammatory cells. There was necrosis of renal cortical tubules and atrophy of glommeruli, vacuolation of mesenchymal glomerulular cells , dilated vacuolated cortical tubules, some with degeneration and loss of epithelial in lumen and degenerative necrotic tubules. In addition there is degenerative and vacuolative changes of myocardial muscle cells, and atrophy of myocardial muscle cells; and there was an evidence of interstitial edema. as well as there is atrophy of white pulp lymphoid tissue and congested of red pulp, also there was increase cellularity of red pulp(present of macrophage cells in red pulp). Furthermore,the present study exposes that the treatment with naproxen sodium would lead to other histo - pathological changes in the stomach and small intestine include vacuolation of mucosal epithelial cells of the stomach and inflammatory cells in the serosa with noticeable presence of prominant ganglion cells in the outer zone of muscularis externa and degeneration of mucosal lining and mucosal glands in the lamina propria of stomach(glandular region). In addition there have been vacuolation and degeneration of mucosal epithelial lining of small intestine , ulceration of the mucosa in the small intestine , increase in length of the villi and infiltration of inflammatory cells in lamina properia and inflammatory cells in the serosa and vacuolation of muscularis externa. In the colon there is vacuolation (prominent mucous gland) and mix of inflammatory cells infiltration, vacuolation (increase mucous gland) and a few mononuclear cells in the lamina propria and there was increase minimal fibrosis.Either in the uterus there was thicken fibrotic lamina propria and few endometrial gland. Finally the pathological changes that have been found for high dose group of naproxen prove to be more severe than both the intermediate and low dose groups.
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دراسة تاثير الخصي في مستويات بعض الهرمونات التناسلية وبعض المعايير الدمية في ذكور الماعز المحلي الاسود Study The Effect of Castration On The Levels of Some Reproductive Hormones , And Some Hematological Parameters In The Local Black Buck

اسم المؤلف: باسم حميد عبد علي
اسم المشرف: ضياء حسين جاسم الدليمي
الموضوع العام: الطب البيطري
الدرجة: ماجستير
اللغة: العربية
السنة: 2011
مكان الجامعة: القادسية
المستخلص: اجريت الدراسة لغرض التعرف على تاثير عملية الخصي في ذكور الماعز المحلي الاسود في مستويات الهرمونات التناسلية والصورة الدمية , وقد اشتملت الدراسة على (25) ذكرا تراوحت اعمارها بين (1.5 - 2.5) سنة وكانت خالية من الامراض والمشاكل التناسلية , وقسمت عشوائيا ال | The current study was conducted for the purpose of identification the effect of the castration in male goats on levels of reproductive hormones and some hematological parameters , and had included (25) males ranged from ages between (1.5 - 2.5) years old and was free from diseases and reproductive problems. The animals in this study were randomly divided into two groups (control group includes (10) male and experimental group includes (15) male) that housed in same conditions of nutrition and management. The levels of reproductive hormones include (Spermatogenesis Stimulating hormone (SSH) , Interstitial cell stimulating hormone (ICSH), testosterone, Estradiol - 17 beta and progesterone) as well as blood parameters that include (red blood cells( RBCs), total white blood cells ( tWBCs), packed cell volume (PCV), hemoglobin concentration (Hb), total protein and cholesterol concentration) were estimated three times before castration and in intervals of two weeks between examination and other , Experimental animals were examined after castration all hormones and blood parameters checked two weeks after castration, then after four weeks from castration, and re - measured all hormones and blood parameters after six weeks of the castration. The method of analysis Radioimmunoassay (RIA) was used to measure the levels of reproductive hormones in animals blood serums also the routine blood tests were use for checking the blood parameters. The results revealed that there are significant increased in the level of ICSH in experimental animals after castration (0.345 ± 0.0413) mIU / ml while the level of testosterone and estradiol - 17 beta significantly decrease in experimental animals after castration and the results were (0.0225 ± 0.0036) ng/ ml and (0.93 ± 0.15) pg/ ml, respectively. While castration did not show any significant effect on the levels of reproductive hormones SSH and progesterone (0.145 ± 0.01) mIU/ ml and (0.295 ± 0.03) ng /ml respectively in experimental group. The result showed that there were significant increased in RBCs , PCV, total protein and cholesterol (17125 ± 335.18) × 10 3 cell/µL , (37.7 ± 0.40) , (6.84 ± 0.25) g /100 ml and (84.41 ± 6.6) mg/100 ml respectively in experimental group after castration, while tWBCs and hemoglobin didn't affect after castration in experimental group (10788 ± 429.39) cell /µL and (11.35 ± 0.180) g/ 100 ml, respectively.
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تشخيص فايروس التهاب القصبات المعدي في حقول دجاج اللحم في محافظة ذي قار باستخدام تقنية الاليزا (ELISA) وتفاعل سلسلة البلمرة المنعكس في الوقت الحقيقي (rRT - PCR) Identification of Infectious Bronchitis Virus In Thi - Qar Province In Broilers Farms By Using ELISA and rRT - PCR

اسم المؤلف: ظافر ياسر عزيز الفاضلي
اسم المشرف: نافع صبيح جاسم
الموضوع العام: الطب البيطري
الموضوع الدقيق: الامراض - الدواجن
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: القادسية
المستخلص: اجريت هذه الدراسة لتقييم مدى انتشار مرض التهاب القصبات المعدي الفيروسي في دجاج اللحم في محافظة ذي قار, قسمت منطقة الدراسة جغرافيا الى خمسة مناطق (شمالية, جنوبية, غربية , شرقية, مركزية) حيث تمت الدراسة على عشرون حقل دجاج لحم غير ملقحة اربعة قاعات من كل منط | The current study was conducted for evaluation of prevalence of IB in broilers in the Thi - Qar province, which geographically divided into five regions, (Northern, Southern, Western, Eastern and central area), twenty broiler farms without history of previous vaccination, four from each region were included in this study. Serum and autopsies were collected from affected birds after recording of the clinical signs that observed on the birds; positive serum samples that detected by rapid test were used for evaluation the antibodies titrations by ELISA technique, while the histological specimens (trachea, lung and kidney) were used for detection of histopathological changes and detection of presence of the IBV infection by molecular approach (real time - PCR). The clinical manifestation includes : typical respiratory sings of IB infections, with depression, rough feather, and assembling near the heat source, as well as some birds were found dead with mortality rate ranging between 30 - 80%. Also 96 serum samples were collected from other broiler farms as following ( 42 asymptomatic vaccinated, 54 asymptomatic non - vaccinated, 26 symptomatic vaccinated) for ELISA and real time PCR tests. The result of rapid test showed that the percentages were different among the areas of study when the southern area revealed the highest percentage (80%) among the others followed by the northern area and western (70%), then central and eastern(65%). The results of ELISA showed a high titer for antibody of IBV in all these farms in areas of the study, but the highly significant results (P<0.05) were found in the southern area(7032.2±1640.92) and then northern area (5722.18±2060.1), a less significant differences were in central and eastern areas (3188.11±742.45; 3161.8±52.6) respectively. In relation to age, the high titer of IB antibodies in total 20 farm by ELISA were recorded in 12 farms at 4 weeks of age (60%) followed by 4 farms at 5 weeks of age (20%), 3 farms at 3 weeks of age (15%) and 1 farm at 2 weeks of age (5%), while there is no recorded infection in farms at 6 ages. According to history of vaccination and clinical manifestation of tested birds, most of the tested flocks showed high level of antibody titers to IBV by ELISA technique in asymptomatic vaccinated chickens samples 38/42 (90%) followed by asymptomatic non vaccinated chickens 8 (14.8%) and symptomatic vaccinated chickens 6(23.07%). While rRT - PCR showed that 69 ( 80%) sample were revealed positive results. The most important gross lesion observed on immediately the necropsy after death were tracheal congestion, caseous exudates in the trachea, pneumonia, airsacculitis, pericarditis and perihepatitis, as well as, nephritis; while The histopathological changes were summarized by desquamation of tracheal epithelial cells and hemorrhage with infiltration of inflammatory cells specially lymphocyte cells, hyperplasia of epithelial cells with hemorrhage, lung sections showed severe infiltration of inflammatory cells mostly lymphocyte cells and severe congestion with spots of pneumonia and the histopathological changes in kidneys were characterized by tubules degeneration , glomerular distention with severe hemorrhage and lymphocyte infiltration.
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الامراضية السمية لكبريتات الثاليوم في الجرذان المختبرية البيضاءSprague Dawley (Rattus norvegicus) Toxicological Pathology of Thallium Sulfate In White Laboratory Spraguedawley Rats (Rattus Norvegicus)

اسم المؤلف: مازن عادل جايان
اسم المشرف: زينب وحيد خضير صالح كاظم مجيد
الموضوع العام: الطب البيطري
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: البصرة
المستخلص: اجريت الدراسة الحالية في البيت الحيواني التابع لكلية الطب البيطري - جامعة البصرة لمعرفة الامراضية السمية لكبريتات الثاليوم على انسجة الجسم المختلفة وبعض المعايير الكيموحيوية للجرذان المختبرية البيضاء Sprague Dawley (Rattus norvegicus). في هذه الدراسة تم | The present study was conducted at the College of Veterinary Medicine - University of Basrah to investigate the effect of Thallium Sulfate on some physiological parameters and histological changes in the Laboratory White Sprague Dawley Rats (Rattus norvegicus). In this study 48 rats were used, that were sexually mature (16 weeks age and 250±50 gm weighing). These animals were divided into four groups each group contain 12 rats. All of these groups administrated orally with : - normal saline ,0.4 ,0.8 ,1.6 mg /kg b. w. of thallium sulfate respectively for 90 days.After ending of this period, all animals were sacrificed after anesthesia by chloroform inhalation for the study. The study includes the effects of thallium sulfate on some biochemical parameters as estimation of serum liver function enzymes ( AST and ALT ) and kidney function enzymes ( Urea and Creatinine ). This study also includes the estimation of serum acetylcholine (Ach) concentration. The present study revealed histopathological effects of thallium sulfate on nervous system, liver, kidneys, eyes, skin, and testis. In addition to its electron microscopic changes were occurred on mitochondria of hepatocyes and cells of proximal convoluted tubules of kidney. The results of this study showed significant increase (P? 0.05) in liver enzymes and kidney function enzymes and acetylcholine concentration in treated groups in comparison with control group. There is also histopathological changes occurred in nervous tissue (brain, spinal cord, and sciatic nerve), liver, kidney, testis, eye, and skin. The ultrastructural changes showed swelling of mitochondria and also an increased in number in rats treated with 1.6 mg/kg b.w. of thallium sulfate in comparison with control group.
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تركيز المعادن الثقيلة في حليب الابقار والاغنام والماعز في محافظـة القادسية The Concentration of Heavy Metals In Milk of Cows , Sheep & Goats In Al - Qadisiya Province

اسم المؤلف: علياء حسن علـي السماوي
اسم المشرف: كريم ناصر طاهر
الموضوع العام: الطب البيطري
الموضوع الدقيق: الصحة العامة
الدرجة: ماجستير
اللغة: العربية
السنة: 2014
مكان الجامعة: القادسية
المستخلص: اجريت هذه الدراسة في محافظة القادسية للمدة من كانون الاول 2012 لغاية اذار 2013 شملت خمسة مناطق مختلفة من محافظة القادسية هي كل من (مركز الديوانية وناحية السنية وناحية الشافعية وناحية الدغارة وقضاء الحمزة) لغرض تحديد مدى تلوث الحليب الخام ببعض المعادن | This study was carried out in AL - Qadissiyah province during the period from December 2012 to March 2013 involved five different regions such as (Diwaniyah city center , Saniyah district , shafeia district , Dagharah district and AL - Hamza district) to determine the level of contamination of raw milk with the toxic heavy metals such as Cadmium (Cd), Lead (Pb), Nikel (Ni), Arsenic (As) and Mercury (Hg). A total of 150 raw milk samples were collected from 150 animals belonged to three species involved (50) cows , (50) sheep and (50) goats selected randomly, ten animals per each species per regions. The results of a statistical analysis of the experimental data showed that : Chemical components of milk 1.There are a significant differences (p <0.05) in all contents of milk among different species of studied animals (cows , sheep and goats). Except fat content for cows and goats milk were not significant different. Sheep's milk had significantly (p<0.05) of (protein, fat, solids not - fat and ash) in comparison to cow's and goat's milk (7.535, 6.617, 12.026 and 0.646) % respectively. Cow's milk had significantly (p<0.05) higher lactose content (4.757%) than both sheep's and goat's milk. components of milk 2. PhysicalThe results revealed that there were significant differences (p <0.05) in all physical characteristics of milk among different species of animals involved in this study (cows, sheep and goats). Sheep milk had significantly (p<0.05) (Titratable Acidity 0.182% and conductivity 3.214 mS/cm) than both cow's and goat's milk , goat's milk contains the highest specific gravity 1.057mg /100 ml while the pH values of cow's , sheep's and goat's milk were similar. Heavy metals in milk - Effect of Species The results revealed that significant differences (P<0.05) among the mean values of heavy metal concentrations included in the study (Cadmium (Cd) , lead (Pb), Nikel (Ni), Arsenic (As) and Mercury (Hg) in milk of different species (cows , sheep and goats). Sheep's milk had significantly levels of such heavy metals (0.251, 0.801, 0.563, 0.283 and 0.124) mg/ l respectively than both cow's and goat's milk while cow's milk had significantly the lower levels (0.098, 0.311, 0.221, and 0.122 , 0.037) mg / l respectively. - Effect of regions : The results showed that significant differences (P<0.05) in the means values of heavy metal concentrations in milk among different regions. The Highest levels of heavy metals were observed in AL - Hamza district (0.184, 0.624, 0.494, 0.246 and 0.119) mg / l respectively and the lowest levels of heavy metals were noticed in Dagharah (0.144 , 0.507, 0.382 , 0.167 and 0.061) mg / l respectively. - Effect of month : The results of the statistical analysis showed that there were non - significant differences in the average concentrations of cadmium, lead and arsenic among months while a significant differences (P <0.05) were observed in the level of nikel between march and other studied months [(0.494) mg / l in march and (0.397, 0.418 and 0.415) mg / l for December, January and February respectively]. Also the results showed that there were a significant differences (P<0.05) in the levels of mercury between march and each of January and February [(0.106) mg / l in march and (0.081 and 0.083) mg / l in January and February respectively]. From the results obtained in the present study,it can be concluded that : 1 - Heavy metals like cadmium (Cd), lead (Pb), Nikel (Ni), Arsenic (As and Mercury (Hg) which distributed among different sites were exceed the maximum acceptable limits set by WHO and EPA. 2 - High concentration of heavy metals (Cd, Pb, Ni, As and Hg) in city center site may be due to the industrial activities. 3 - High concentration of heavy metals in the other sites may be due to application of waste water for agriculture purposes (irrigation) which pollute the soil and also be allowing toxic metals to enter in to the food chain posing a serious health hazard.
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التحري عن اهم الامراض الفيروسية التنفسية في دجاج اللحم باستخدام فحص الاليزا (ELISA) وتقنية تفاعل سلسلة البلمرة المنعكس في الوقت الحقيقي (rRT - PCR) في محافظة الديوانية Detection of Certain Viral Respiratory Diseases In Broiler Chickens By ELISA And Real Time RT - PCR Technique In Al - Diwaniyia Province

اسم المؤلف: عباس هادي جاسم المحمودي
اسم المشرف: نافع صبيح جاسم
الموضوع العام: الطب البيطري
الموضوع الدقيق: الامراض - الدواجن
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: القادسية
المستخلص: تعد انفلونزا الطيور والتهاب القصبات المعدي والنيوكاسل من الامراض المهمة اقتصاديا في صناعة الدواجن.صممت الدراسة الحالية لتسليط الضوء على دور فيروسات ND, IB, AI , ومعرفة مدى انتشارها في احداث الاوبئة التنفسية في دجاج اللحم باستخدام تقنية الاستنساخ المن | Avian influenza, Newcastle disease and Infectious bronchitis are diseases that have economical importance in poultry industry. This study was designed to clarify the roles of IBV, AIV and NDV in an outbreaks of respiratory diseases in broiler chickens by using ELISA and real time RT - PCR assay in Al - Diwaniyia province , Iraq. A total of 30 commercial broiler flocks with high mortality (20 to 80 %) in Al - Diwaniyia province were investigated.Tracheal swabs and tissue specimens ( Trachea, lung, kidney and cecal tonsils ) were tested initially by rapid test for detection of Infectious bronchitis virus ( IBV ) , Influenza type A virus ( AIV) and Newcastle disease virus (NDV).The total results of rapid immunochromatography assay showed that out of 30 flocks 28 ( 93.33%) were positive for IBV and 21(70%) flocks were positive for AIV while 4(13.33%) flocks were positive for NDV. Blood samples were collected for detection of antibodies against IBV, AIV & NDV in serum samples , the results of indirect ELISA showed that out of 30 flocks were 30(100%) gave positive result with indirect ELISA test for IBV and 23(76.67%) flocks were positive for AIV (H9 ) whereas 5(16.67%) flocks showed positive result for NDV. The result of rRT - PCR showed out of 30 (100%) flocks were positive for IBV and 23(76.67%) positive for AIV (H9) whereas 5(16.67%) flocks were gave positive results for NDV. In conclusion the high positivity in the examined broiler flocks were mainly due to mixed infections of IBV, AIV(H9) and NDV, However we concluded that IBV and AIV were most important causes of respiratory diseases in this study. Also this study demonstrated that Real time qRT - PCR technique and ELISA were rapid and accurate as diagnostic tool in early detection of IBV, AIV and NDV.
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التاثيرات السمية المرضية للسايبرمثرين على بعض المعايير الكيموحيوية, وفعالية الاستايل كولين في الجرذان البالغة Toxicopathological Effects of Cypermethrine On Some Biochemical Parameters And Acetylecholine Activity In Sprague Dawley Rarts (Ruttus Norvegicus)

اسم المؤلف: طلال جبل حسين
اسم المشرف: زينب وحيد خضير صالح كاظم مجيد
الموضوع العام: الطب البيطري
الموضوع الدقيق: الطب البيطري
الدرجة: ماجستير
اللغة: الانكليزية
السنة: 2014
مكان الجامعة: البصرة
المستخلص: اجريت الدراسة الحالية في البيت الحيواني التابع لكلية الطب البيطري في جامعة البصرة لتقييم التاثير السمي للجرع المختلفه للسايبرمثرين على الدماغ والحبل الشوكي والعصب الوركي والكبد والكلية والخصية اضافة الى تاثيره على انزيمات الكبد(AST,ALT) وانزيمات الكلية (ا | The present study was conducted in the laboratory animal house - college of veterinary medicine - University of Basra to investigate the Toxicological effects of different doses of Cypermthrin on Brain, Spinal cord ,Sciatic nerve, Liver, Kidney ,and Testis and also its effect on liver enzymes (ALT and AST), kidney enzymes (urea and creatinine) ,and serum acetylcholine (Ach) in adult Sprague dawley rats (Rattus norvegicus). Fourty eight adult rats were used , they were divided randomly and equally into four groups. Control group orally dosed with normal saline for 90 days. The other three groups were dosed orally with different three doses of cypermthrin , high dose ( 64 mg / kg b.w. ) intermediate dose ( 32 mg / kg b.w. ) and low dose ( 16mg / kg b.w. ). The results showed that there were hitopathological changes of brain, spinal cord and Sciatic nerve that revealed there were a dose dependent increase in vaccuolation in nerves fibers to be affect larg number of nerve fiber in high dose and also affected few numbers of nerve fibers in low dose. Also there were changes in liver ,kidney and testis. Serum AST, ALT, urea, creatinine and acetylecholine concentrations increased significantly ( p ? 0.05) in rats exposed to cypermethrin in comparisons with control. In conclusion cypermthrin affects positivly on histopathological findings of nervous system, liver tissues and enzymes, kidney tissues and enzymes ,and Acetylcholine (Ach) neurotransmitter.
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دراسة شكلية ونسجية وكيمياء نسجية للمعدة في الجاموس المحلي البالغ Bubalus bubalis Histomorphological And Histochemical Study of Stomach of The Adult Local Buffalo Bubalus Bubali

اسم المؤلف: سوسن غفوري احمد
اسم المشرف: فوزي صدام محسن الاسدي
الموضوع العام: الطب البيطري
الموضوع الدقيق: التشريح والانسجة والاجنة
الدرجة: ماجستير
اللغة: العربية
السنة: 2015
مكان الجامعة: البصرة
المستخلص: اجريت هذه الدراسة على عشرين معدة للجاموس المحلي الخالية من الامراض والتي جمعتمن مجزرة العـــشار في البصرة. تضمنت الدراسة الشكلية قياسات متعددة للاجزاء الاربعة من المعدة كالطول والارتفاع علاوة على حساب معدل عدد الحليمات الموجودة في الكرش في السنتمتر ال | 20 Stomach of healthy stomach of local buffalo were used in present study which collected from Al. Ashar Massacre in the city of Basrah.morphological study included several measurements for all parts of Stomach like length and width in addition to calculate the average of number of papillae that present in rumen per each Square centimeter and the average of length of these papillae as well as find the diameters of openings between chambers of Stomach. While the histological part was comprise investigate the histological structure of the wall of chambers of Stomach. Histological Section were prepared from these chambers stained with hematoxylin and eosin Stain to Study the general characters of tissue, while other sections were Stain with special Stains to detect the histochemical properties of these tissues. The morphological study showed that the stomach of buffalo formed from four chambers these are rumen, reticulum , omasum and abomasum differs in their length and width. The inner lining of rumen was contained on many papillae the average of length was 800 micrometers while their average of number was 118 papilla /cm2. while the inner lining of reticulam was included several number of lamellae which arranged in form like honeycomb. Omasal lining showed crescent lamella differs in their number and length while the wall of abomasums is divided in to two regions real gastro glands origin region and the color red and has a helical folds the seconed region is the pyloric glands region, a light colored region and the container on the irregular folds. In Histological part the present study revealed that the wall of all the chamber in buffalos, stomach were histologically composed from four layers; these are tunica Mucosa , Submucosa , Muscularis and Serosa.The tunica Mucosa of rumen was characterized by of papillae that differs in their number and length according to its region in rumen while the glands were abscent in lamina properia as well as abscent of muscularis mucosa, while the wall of abomasums appeared divided histologically into 3 regions ; cardiac , Fundic and pyloric region About the histochemical part , this study demonstrated that the collagen fibers were prevalent more density in Submucosal tunica than other histological tunicas in wall of rumen ,in contrast the elastic fibers were highly constricted in muscular tunica of the rumen and mucosal larger of reticulum in comparion with other tunicas and Submucosa of the Ommasum ,the elastic fibers showed prevalenced in all layers of the cardic region of the abomasums. this study showed prevalence of keratin in the mucosa denser than other layers in the rumen , reticulum and omasum while abscent in abomasum. this study also showed prevalence of glycogen and proteins in all parts of rumen as well as prevalence of fat and alkaline phosphatase activity in the wall of all chambers of stomach.
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دراسة تشخيصية وامراضية نسجية للقناة الهضمية في الدجاج المنزلي Gallus gallus domasticus المصابة بالطفيليات المعوية في مدينة البصرة Diagnostic And Histopathological Study of Intestinal Tract of Domestic Fowl Gallus Gallus Domesticus Infected With Intestinal Parasites At Basrah City

اسم المؤلف: اسراء صبيح بداي الغنامي
اسم المشرف: غازي يعقوب عزال الامارة
الموضوع العام: الطب البيطري
الدرجة: ماجستير
اللغة: العربية
السنة: 2013
مكان الجامعة: البصرة
المستخلص: امتدت الدراسة الحالية من شهر تشرين الاول 2011 ولغاية شهر اذار 2012 , حيث جمعت خلالها 108 نموذجا بواقع 36 عينة من الذكور و72 عينة من الاناث من الدجاج المحلي1758) Gallus gallus domesticus (Linnaeus, من محافظة البصرة بهدف عزل الديدان المتطفلة على القناة الهض | The recent study extended from October 2011 to January 2012, collected 108 samples by 36 samples of males and 72 samples of female of local chickens Gallus gallus domesticus (Linnaeus, 1758)from Basrah to isolate parasitic worms on the gastrointestinal tract and diagnosed as well as study percentage and severity of the infection, as well as monitoring biological activities of isolated parasites and find out macroscopic and pathological changes caused by these worms and find out the impact of tapeworms on the values of the blood parameters of chicken. The results showed infected of chicken with five types of tapeworms (Hymenolepis carioca, Raillietina tetragon, Raillietina cesticillus, Choanotania infundibulum, Cotugina sp) with percent of infection and severity reached 4.62%, 11.4; 19.44%, 12.09; 5.55%, 12.66; 9.25%, 12.6; 6.48 %, 11.14 respectively and got one type of nematodes Subulura brumpti with percentage of infection and severity reached 10.18%, 91 and three types of trematode Echinostoma revulatum, Hypoderm coniodium, Psilolecithum longorchum with percent of infection and severity reached 1.85%, 15.5; 0.92%, 10; 0.92% , 7 respectively.The results of the recent study showed that infection with two types is the most common reaching incidence of 25.92%, and study results indicated that there were significant differences between males and females in susceptibility to intestinal worms.Observed by studying the sites of worms in the gut that the proportion of tapeworms in the small intestine are more than in the large intestine while recorded nematodes only in the large intestine As for worms Trematode has found in the small intestine. The results also included monitoring biological activities of isolated parasites from the gastrointestinal tract from the beginning of the division of the egg and develop into a larva has been observed that the nematodes have the ability to survive outside the host, who were present more than tapeworms.the study contained a physiologic side on the impact of tapeworms on some blood parameters where showed infection of domestic fowl with anemia by observing the decrease in the number of red blood cells R.BC , hemoglobin Hb and P. CV while there was an increase in the number of white blood cells WBC. The study recorded the number of grossly and histological lesions in the intestine and the cecum of domestic chicken Because they infected with tapeworms, nematodes and flukes. grossly lesions included blockage and swelling in the intestines infected with tapeworm and cecum infected with nematode and appearance of rough nodules on the walls of the intestine infected with tapeworm with hemorrhage and the appearance of yellowish green purulent material in the inner wall of the intestines, and the histological changes has included atrophy of intestinal villi with sloughing and desquamation of epithelial layer with appearance of inflammation in the form of pseudomembrane and infiltration of inflammatory cells and coaglative necrosis, it have seen hemorrhage and superficial erosion in the mucous layer and sloughing of villi and epithelial layer with hyperplasia appearance purulent cells and destruction of intestinal glands and ulceration in the mucous layer in addition to the congestion
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