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The Role of Cyclosporine In Intra Bone - Bone Marrow Transplantation In Male Rabbits
Author name:
عادل جاسب عويد
Supervisor name:
Abdulbari A. Alfaris | Alaa A. AL Sawad
General topic:
Veterinary Medicine
Specific topic:
Surgery
Degree:
Master
University:
University Of Basrah - College Of Veterinary Medicine - Department Of Surgery And Veterinary Obstetrics
Language:
English
University location:
Basrah
First pages:
21T525 - p.pdf
Abstract:
استخدم فيها 30 ارنبا ذكرا حيث قسمت الى ثلاثة مجاميع متساوية بالعدد كل مجوعه تحوي على عشرة ارانب اثنان منها تم اختيارها كواهب لنخاع العظم والثمانيه الاخرى كانت مستلمه, وكانت المجموعه الاولى هي مجموعه سيطرة وقد اعطيت المحلول الملحي الطبيعي ,والمجموعه الث | Thirty male rabbits were randomly divided into three groups as following : Group one (sham group) consists of ten male rabbits, two of them were used as donors and other eight were administered 12.5ml/kg/BW normal saline (N.S) orally for ten days. At Zero time of the experimental the collection of blood samples (10ml) was carried out on each animal from the heart (cardiac puncture) and by a sterile syringe 22 Gage needle. The (10ml) amount of blood was divided into two parts. (5ml) was deposited into tubes without anticoagulant and then refrigerated for a maximum of 12h and centrifuged at (5000 rpm) for 15 minutes these serum samples were stored in polyethylene eppendorff tubes at - 20?C Estimation of Total interleukin 2 in serum by using an Enzyme - Linked Immunosorbent Assay (ELISA). The 2nd sample was also (5ml) taken for performing hematological test to measure the WBCs account and their differential, RBCs account, Hb & P.C.V. At Day10 of experimental was collecting the blood as similar procedure at zero time and did a similar examination before operation. At Day11 of the experimental an allogeneic Intra - bone - marrow injection of bone marrow transplantation aspiration method (IBM - BMT) AM was carried out and rabbits were re administered with normal saline after surgical operation until Day21 of experimental. Following that rabbits were anesthetized with Ketamine (20 mg/Kg/BW) and xylzine (9 mg/Kg/BW) intra muscular. Before bone aspiration for bone marrow histological test the smears were stained with Giemsa stain. At the end of this experimental study of there was collection of blood as a same procedure at zero time and DAY10 then made the same test to estimate of blood values and interleukin 2. Then all recipients’ rabbits were sacrificed and bled to death to obtain the Kidney and liver, which were dissected out, trimmed and processed for the histological study with used Heamatoxyline and Eosin Stain. Group two (treated with 12.5ml/kg/BW cyclosporine orally administrated) and same protocol of the sham group was applied except when using (treatment with 12.5ml/kg/bw cyclosporine orally administrated) replacing the normal saline. Group three (treatment with 25ml/kg/BW cyclosporine orally administrated) and same protocol of sham group was applied except when using (treated with 25ml/kg/BW cyclosporine orally administrated) replacing the normal saline. The results of the present study Conclusions cyclosporine effects on the bone marrow, immune system because of decrease of WBCs, lymphocytes, neutrophils and interleukin 2.The study Conclusions cyclosporine caused many adverse effects, where by it caused severe liver and kidney; therefore it was considered predisposing factor to infection with several diseases as well as it is considered the main cause of disorders in renal function renal dysfunction. The results of the present study Conclusion the surgical operation of intra - bone - bone marrow transplantation more safe than other ways of intra vinous bone marrow transplantation also the study the operation increase the hematopoietic cells
