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التحري عن اهم الامراض الفيروسية التنفسية في دجاج اللحم باستخدام فحص الاليزا (ELISA) وتقنية تفاعل سلسلة البلمرة المنعكس في الوقت الحقيقي (rRT - PCR) في محافظة الديوانية == Detection of Certain Viral Respiratory Diseases In Broiler Chickens By ELISA And Real Time RT - PCR Technique In Al - Diwaniyia Province
Author name:
عباس هادي جاسم المحمودي
Supervisor name:
نافع صبيح جاسم
General topic:
Veterinary Medicine
Specific topic:
Diseases - Poultry
Degree:
Master
University:
University of Al-Qadisiyah - College Of Veterinary Medicine
Language:
English
University location:
Qadisiyah
First pages:
21T513 - p.pdf
Abstract:
تعد انفلونزا الطيور والتهاب القصبات المعدي والنيوكاسل من الامراض المهمة اقتصاديا في صناعة الدواجن.صممت الدراسة الحالية لتسليط الضوء على دور فيروسات ND, IB, AI , ومعرفة مدى انتشارها في احداث الاوبئة التنفسية في دجاج اللحم باستخدام تقنية الاستنساخ المن | Avian influenza, Newcastle disease and Infectious bronchitis are diseases that have economical importance in poultry industry. This study was designed to clarify the roles of IBV, AIV and NDV in an outbreaks of respiratory diseases in broiler chickens by using ELISA and real time RT - PCR assay in Al - Diwaniyia province , Iraq. A total of 30 commercial broiler flocks with high mortality (20 to 80 %) in Al - Diwaniyia province were investigated.Tracheal swabs and tissue specimens ( Trachea, lung, kidney and cecal tonsils ) were tested initially by rapid test for detection of Infectious bronchitis virus ( IBV ) , Influenza type A virus ( AIV) and Newcastle disease virus (NDV).The total results of rapid immunochromatography assay showed that out of 30 flocks 28 ( 93.33%) were positive for IBV and 21(70%) flocks were positive for AIV while 4(13.33%) flocks were positive for NDV. Blood samples were collected for detection of antibodies against IBV, AIV & NDV in serum samples , the results of indirect ELISA showed that out of 30 flocks were 30(100%) gave positive result with indirect ELISA test for IBV and 23(76.67%) flocks were positive for AIV (H9 ) whereas 5(16.67%) flocks showed positive result for NDV. The result of rRT - PCR showed out of 30 (100%) flocks were positive for IBV and 23(76.67%) positive for AIV (H9) whereas 5(16.67%) flocks were gave positive results for NDV. In conclusion the high positivity in the examined broiler flocks were mainly due to mixed infections of IBV, AIV(H9) and NDV, However we concluded that IBV and AIV were most important causes of respiratory diseases in this study. Also this study demonstrated that Real time qRT - PCR technique and ELISA were rapid and accurate as diagnostic tool in early detection of IBV, AIV and NDV.
