مشاركة
عزل وتشخيص البكتريا العنقودية السالبة لانزيم الخثرة من حالات مرضية مختلفة ودراسة مقاومتها للمضادات الحيوية == Isolation And Identification of Coagulase Negative Staphylococci From Different Clinical Cases And Study of Their Antibiotic Resistance
اسم المؤلف:
مجاهد خلف علي احمد الجميلي
اسم المشرف:
عباس علي هادي الدجيلي | وعد محمود رؤوف
الموضوع العام:
علوم الحياة
السنة:
2008
الموضوع الدقيق:
التقنيات الاحيائية
الدرجة:
ماجستير
الجامعة:
جامعة تكريت - كلية التربية - قسم علوم الحياة
اللغة:
العربية
مكان الجامعة:
صلاح الدين
الصفحات الاولى:
24T2645 - p.pdf
المستخلص:
جمعت 265 عينة من مستشفى تكريت التعليمي العام وشملت هذه العينات عينات سريرية لمرضى راقدين في المستشفى ومرضى مراجعين لها. اخذت العينات من المرضى المشتبه باصابتهم بخمج السبيل البولي وخمج الجروح والحروق وخمج الاذن الوسطى ولكلا الجنسين وباعمار مختلفة. انجزت هذ | A number of 265 samples had been collected from Tikrit Teaching Hospital (T.T.H.).These samples were taken from inpatients and outpatients suspected to have Urinary Tract Infection, Wound & Burn Infections and Mid - Ear infection for males and females in varied ages. The practical part had been fulfilled during the period from the beginning of December 2006 till the beginning of March 2007.Isolates diagnosed were identified depending on morphological and cultural characteristics. The results revealed 60 Staphylococci isolates, which shows 27 isolates (45%) were S. epidermidis, 13 isolates (21.66%) S. saprophyticus, 10 isolates (16.67%) S. xylosus, 3 isolates (5%) S. lentus, two isolates (3.33%) S. heamolyticus, and one isolate (1.67%) for each of S. simulans and S. hominis Ten (34.48%) of patients are located in the age group (15 - 30) years. while concerning the classification according to sex, females were (65.5%). Otitis media and Wounds and Burns infections, their ratio was high (61.1%, 53.8%) consequently both in males in comparison with females. Concerning the relationship between the infection and the residence and the level of education, the results revealed that isolation of Coagulase Negative Staphylococci (CoNS) were high in rural areas and in uneducated persons regarding the various infections than those live in urbans and educated ones. A of main tests were carried out in order to achieve the purpose of this study according to the planned goals, some of which are the virulence factors of Coagulase negative Staphylococci. These include DNase, Urease, Lipase, Lecithinase, and Alkaline phosphatase.The results revealed the ability of 67.5% of isolates to produce Alkaline phosphatase, and 46.6% of isolates to produce Urease, also 27.5% of isolates were able to produce Lecithinase , and the ability of 19.16% to produce Lipase, while 22.5% of isolates were capable of producing DNase. In addition to these tests, the test for producing ? - lactamase was done, 85% of isolates were positive to this test because ? - lactamase is one of the significant enzyme which are produced by bacteria since it improves the resistance of bacteria for antibiotics. Thirteen different antibiotics used for the Antibiotics susceptibility tests which show that most effective antibiotic is Nitrofurantoin (95%), followed by Amikacin (93.3%), Novobiocin(90%), Chloramphenicol(88.3%), Rifampicin (78.5%), Vancomycin (75%), Ciprofloxacin (73.3%), Clindamycin (70%), Gentamicin (58%), Penicillin G (50%), Trimethoprim (50%), Tetracycline (40%) and Cefoxitin (18.3%).
