Share
دراسة التاثير العلاجي لحليب الافراس المخمر للتقليل من تاثير الاجهاد التاكسدي المستحدث بواسطة خلات الرصاص في الجرذان البيض == Study The Therapeutic Effect of Fermented Mare? S Milk To Reduce The Effect of Oxidative Stress Induced By Lead Acetate In White Albino Rats
Author name:
شيماء عباس صبيح
Supervisor name:
كريم ناصر طاهر
General topic:
Veterinary Medicine
Specific topic:
Public Health
Degree:
Master
University:
University of Al-Qadisiyah - College Of Veterinary Medicine
Language:
Arabic
University location:
Qadisiyah
First pages:
21T528 - p.pdf
Abstract:
اجريت الدراسة الحالية في البيت الحيواني التابع لكلية الطب البيطري / جامعة القادسية بهدف تقييم الدور العلاجي لحليب الافراس المخمر في التقليل من تاثير الاجهاد التاكسدي المستحدث بواسطة خلات الرصاص. استخدم في هذه الدراسة ( 40 ) ذكر من الجرذان البيضاء نوع ( w | The present study was conducted at the animal house of veterinary medicine college of AL - Qadisiyah University during the period from 27 - 2 - 2014 to 30 - 3 - 2014. to evaluate the productive role of fermented mare's milk in to reduce the oxidative stress that induced by lead acetate , 40 male albino rats weighing between 200 - 210 gm were used in this study. the animals were obtained from the animal house of veterinary medicine College / Baghdad University. The animals were randomly distributed into four equal groups , ( 10 rats per each group / cage) as follow : - Control group : The rats were given only basal diet and deionized drinking water - - First treatment group ( T1) : The rats were given basal diet and deionized drinking water that contain 1000 ppm lead acetate for 30 days. - Second treatment group ( T2 ) : The rats were administrated basal diet and deionized drinking water that contained 1000 ppm of lead acetate and received orally ( by gavag ) fermented mare's milk at a dose ( 10ml / kg b.w / day ) for 30 days. - Third treatment group (T3) : The rats were given basal diet and deionized drinking water that contained 1000 ppm of lead acetate and received orally ( by gavag ) EDTA at a dose ( 50 mg/ kg b.w / day ) for 30 days. At the end of the experiment all the animals were fasted for ( 12 hours ) and then killed animals from each treatment group blood samples were collected from the heart to measures some blood parameters such RBC , Hb and PCV and biochemical parameters such as liver function parameters ( total protein and activity of some enzymes such as ALT , AST , ALP and LDH ) , kidney function parameters such as Urea and Creatinine concentration and oxidative stress parameters such as GSH , SOD , MDA ). In addition to that study the histopathological changes of liver and kidney tissues were studied The results of the present study can be summarized as follow : - 1 - Blood parameters : The results of the statistical analysis showed that there was a significant ( p < 0.05 ) decrease in the mean values of RBC , Hb , and PCV in a group treated with lead acetate only ( T1 ) as compared to other experimental groups. while groups treated with EDTA and fermented mares milk showed a significant ( p < 0.05 ) improvement in blood parameters. 2 - Biochemical parameters : A - Liver function parameters : The result revealed that there was a significant ( p < 0.05 )effect of lead acetate on level of total protein where caused a decrease in total protein concentration and increase the in activity of ALT , AST , ALP , and LDH as compared to other experimental groups. Yet treatment with fermented mare's milk ( T2 ) resulted in an increase in the total protein concentration and a decrease in the activity ALT , AST , ALP AND LDH. Also treatment with EDTA ( T3 ) caused a similar results to ( T2 ). B - Kidney function parameters : The results obtained in this study demonstrated that a significant ( p < 0.05 ) increase in the level of urea and creatinine in a group of animals treated with lead acetate only ( T1) , while using fermented mare's milk ( T2 ) and also EDTA contributed reduction in the level of urea and creatinine 3 - Oxidative stress parameters : The results of the present study showed that there was a significant ( p < 0.05) decrease in the concentration of GSH and SOD a significant ( p < 0.05 ) increase in MDA concentration in animals that administrated lead acetate only ( T1) while administration fermented mare's milk and EDTA resulted in significant ( p < 0.05 ) increase in the concentration of GSH and SOD while a significant ( p < 0.05 ) reduction in MDA concentration. The values becomes close to the concentration of the control group. 4 - Histopathological changes : The results of Histopathological examination for tissues of liver and kidney of rats given lead acetate alone ( T1 ) showed that there was a clear Histopathological changes presented in degeneration of hepatocytes and congestion in central vein which associated with hemorrhage in liver and kidney tissues of animals administrated fermented mare's milk showed normal and free from congestion.
