تشخيص فايروس التهاب القصبات المعدي في حقول دجاج اللحم في محافظة ذي قار باستخدام تقنية الاليزا (ELISA) وتفاعل سلسلة البلمرة المنعكس في الوقت الحقيقي (rRT - PCR) == Identification of Infectious Bronchitis Virus In Thi - Qar Province In Broilers Farms By Using ELISA and rRT - PCR
Author name:
ظافر ياسر عزيز الفاضلي
Supervisor name:
نافع صبيح جاسم
General topic:
Veterinary Medicine
Specific topic:
Diseases - Poultry
Degree:
Master
University:
University of Al-Qadisiyah - College Of Veterinary Medicine
Language:
English
University location:
Qadisiyah
First pages:
21T516 - p.pdf
Abstract:
اجريت هذه الدراسة لتقييم مدى انتشار مرض التهاب القصبات المعدي الفيروسي في دجاج اللحم في محافظة ذي قار, قسمت منطقة الدراسة جغرافيا الى خمسة مناطق (شمالية, جنوبية, غربية , شرقية, مركزية) حيث تمت الدراسة على عشرون حقل دجاج لحم غير ملقحة اربعة قاعات من كل منط | The current study was conducted for evaluation of prevalence of IB in broilers in the Thi - Qar province, which geographically divided into five regions, (Northern, Southern, Western, Eastern and central area), twenty broiler farms without history of previous vaccination, four from each region were included in this study. Serum and autopsies were collected from affected birds after recording of the clinical signs that observed on the birds; positive serum samples that detected by rapid test were used for evaluation the antibodies titrations by ELISA technique, while the histological specimens (trachea, lung and kidney) were used for detection of histopathological changes and detection of presence of the IBV infection by molecular approach (real time - PCR). The clinical manifestation includes : typical respiratory sings of IB infections, with depression, rough feather, and assembling near the heat source, as well as some birds were found dead with mortality rate ranging between 30 - 80%. Also 96 serum samples were collected from other broiler farms as following ( 42 asymptomatic vaccinated, 54 asymptomatic non - vaccinated, 26 symptomatic vaccinated) for ELISA and real time PCR tests. The result of rapid test showed that the percentages were different among the areas of study when the southern area revealed the highest percentage (80%) among the others followed by the northern area and western (70%), then central and eastern(65%). The results of ELISA showed a high titer for antibody of IBV in all these farms in areas of the study, but the highly significant results (P<0.05) were found in the southern area(7032.2±1640.92) and then northern area (5722.18±2060.1), a less significant differences were in central and eastern areas (3188.11±742.45; 3161.8±52.6) respectively. In relation to age, the high titer of IB antibodies in total 20 farm by ELISA were recorded in 12 farms at 4 weeks of age (60%) followed by 4 farms at 5 weeks of age (20%), 3 farms at 3 weeks of age (15%) and 1 farm at 2 weeks of age (5%), while there is no recorded infection in farms at 6 ages. According to history of vaccination and clinical manifestation of tested birds, most of the tested flocks showed high level of antibody titers to IBV by ELISA technique in asymptomatic vaccinated chickens samples 38/42 (90%) followed by asymptomatic non vaccinated chickens 8 (14.8%) and symptomatic vaccinated chickens 6(23.07%). While rRT - PCR showed that 69 ( 80%) sample were revealed positive results. The most important gross lesion observed on immediately the necropsy after death were tracheal congestion, caseous exudates in the trachea, pneumonia, airsacculitis, pericarditis and perihepatitis, as well as, nephritis; while The histopathological changes were summarized by desquamation of tracheal epithelial cells and hemorrhage with infiltration of inflammatory cells specially lymphocyte cells, hyperplasia of epithelial cells with hemorrhage, lung sections showed severe infiltration of inflammatory cells mostly lymphocyte cells and severe congestion with spots of pneumonia and the histopathological changes in kidneys were characterized by tubules degeneration , glomerular distention with severe hemorrhage and lymphocyte infiltration.
