عزل وتشخيص النمط المصلي O78 : K80 لاشريشيا القولون الممرضة للطيور من دجاج اللحم ودراسة اصابتها تجريبيا == Isolation And Identification of Avian Pathogenic E. Coli O78 : K80 Serotype From Broilers And Study of Its Experemintal Infection
Author name:
رجاء عبد الزهرة علي
Supervisor name:
علي عبد سهم المياح
General topic:
Veterinary Medicine
Specific topic:
Diseases - Poultry
Degree:
Master
University:
University Of Basrah - College Of Veterinary Medicine - Department Of Diseases And Poultry Diseases
Language:
English
University location:
Basrah
First pages:
21T527 - p.pdf
Abstract:
الايشريشيات القولونية مسئولة عن احداث حالات مرضية مختلفة تسبب خسائر اقتصادية ناتجة عن الهلاكات العالية وانخفاض معدل التحويل الغذائي والخسائر في اتلاف الجثث والاعضاء المصابة بعد الذبح.تم في الدراسة الحالية فحص 70عينة من افراخ فروج اللحم الهالكة او التي كا | Escherichia coli (E. coli) is responsible for a variety of diseases conditions which cause high economic losses in poultry due to high mortalities, decreased food conservation rate and condemnation of whole affected carcass or organs after slaughter. In the present study, 50 out of 70 broiler chickens which have been collected from diseased chickens were exhibited lesions of fibrinous perihepatitis, fibrinous pericarditis and/or airsacculitis. Birds with these lesions were subjected for bacteriological examination. The examination revealed isolation of 23 E. coli isolates with incidence of 46%. Concerning the virulence factors , Congo red binding activity of these isolates reveled detection of 6 positive isolates with incidence of 26.08%. Serotyping showed that out of 6 Congo red positive strains, only 3 strains were serologically typed and were belonged to the serotype O78 : K80. Detection of pathogenic E. coli serotypes was confirmed by PCR technique with specific primers for fimA and fimH genes. All these 3 isolates of the serotype O78 : K80 were reacted with these 2 genes. Antimicrobial suscebtibility pattern displayed sensitivity of these isolates to Chloramphinicol and Gentamicin and their resistance to Amoxicillin, Erythromycin and Nalidixic acid. Experimental infection has been made with this isolate. Two groups of broiler chickens were used the first at one day old and the second at 5 weeks of age. Signs, lesions, histopathological changes were revealed that the first experiment was differed from the second through the presence of pus in the bursa of Fabricius, but hemorrhage in the lungs tissue occur in two experiments.
