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تاثير انزيم SNase المنقى من العنقودي في الغشاء الحياتي للايشيركية القولونية والكليبسيلا الرئوية == Impact of Snase Purified From Staphylococcus Aureus On Biofilm of Klebsiella Pneumoniae And Escherichia Coli

Author name: هند تحسين ابراهيم
Supervisor name: حارث جبار فهد المذخوري
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: SDS - PAGE showed that a single sharp band with an approximately 16.8 KDa molecular weight has been seen. A matter indicates that the enzyme is consistently pure. PCR technique was applied to approve the existence of nuc gene in S.aureus Nevertheless, only those which depicted positive results on DNase agar harboured nuc gene, as it is specified by single band appearance of nuc at assumed molecular weight (ca. 255 pb) of marker that being used. The current study findings highlighted the participation of SNase purified from S. aureus in significant (P< 0.05) preventing biofilm formation by E. coli and K. pneumoniae compared with untreated controls. Evidently, the inhibitory effect of SNase on biofilm formation is undoubtedly perceived, indicating the degradation of the structural major component of biofilm formation (i.e. extracellular DNA). Results revealed that SNase was able to significantly (P< 0.05) reduce the number of the uropathogens; E. coli and K. pneumoniae attached to the uroepithelial cells. 1 Chapter one : Introduction and literature Review 1. Introduction and Literature Review 1.1. Introduction Staphylococcus aureus is a persistent human pathogen that is responsible for a range of diseases that vary widely in clinical presentation and severity. The pathogenesis of S. aureus infection is a complex process involving a diverse array of secreted and surface - associated virulence determinants that are coordinately expressed at different stages of infection (Loughman et al., 2009). Escherichia coli is a genetically diverse species that causes a variety of infections which fulfill many or all of the proposed criteria for biofilmassociated infections (Kaper et al., 2004). Klebsiella pneumoniae is an opportunistic pathogen responsible for a wide range of nosocomial infections. One important factor associated with virulence in K. pneumoniae is its capacity to adhere to surfaces and form biofilms (Cruz et al., 2012). Bacteria often exist as sessile communities called biofilms which are exquisite structures caused by a genetically programmed developmental process. It is estimated that biofilms are involved in 65% of human bacterial infections, since cells in biofilms are 1000 times more resistant than cells in the planktonic state, making medical treatments fail (Shal? et al., 2011). Extracellular DNA (eDNA) plays a significant role in biofilm formation, as revealed by studies in several bacteria including E. coli (Nakao, 2012) and K. pneumoniae (Whitchurch et al., 2002); however, there is no definite proof on a cause - and - effect relationship between DNA release and biofilm formation (Beenken et al., 2012) or becomes a key component of the macromolecular scaffold in many different biofilms (Jakubovics et al., 2013). In rare cases eDNA has been shown to inhibit bacterial settlement. Therefore, it is possible that nuclease mediated eDNA degradation would therefore promote adhesion. Consequently, it is not clear whether microbial nucleases contribute to the gross biofilm structure in clinically relevant situations (Sheilds et al., 2013). 2 Chapter one : Introduction and literature Review All previous studies used both commercial bovine and recombinant human DNaseI in the disruption of medically important biofilms; whilst, extracellular nuclease of S. aureus (SNase) was used against biofilm of S. aureus themselves (Benenken et al., 2012b ; Kiedrowski et al., 2014). Thus, to date, the role of S. aureus exonucleases in biofilm of other bacteria remains unclear. However, in the present study SNase is used against biofilm of other bacterial species (viz. K. pneumoniae and E. coli). To address this, the following steps were undertaken : 1 - Investigating the negative impact of SNase extracted from S. aureus on K. pneumoniae and E.coli biofilm formation. 2 - Studying the preventive activity of SNase on the adherence of K. pneumoniae and E. coli on uroepithelial cell.

دراسة وبائية وسريرية لمرض الجيارديا Giardiasis في اطفال مدينة الشرقاط وضواحيها == Parasitic Study of Giardiasise Among Children In Sharqat City

Author name: علي حمد طلال موسى الجبوري
Supervisor name: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة لبيان تاثير طفيلي الجيارديا المعوي على صحة الاطفال في قضاء الشرقاط لـ(250) طفل، تم اختيارهم بصورة عشوائية، اذ تراوحت اعمارهم من شهر - 15 سنة وللمدة ما بين شهر تشرين الاول 2006 لغاية شهر تشرين الاول 2007، تم فحص عينات الغائط من كل طفل بال

تاثير المستخلص الكحولي للبروبولس على بعض الفعاليات الحيوية في الارانب المصابة بالداء السكري == Effect of Ethanolic Extract of Propolis On Some Biological Activities In Diabetic Rabbits (Oryctolagus Cuniculus)

Author name: دعاء عبد الزهرة دلي الفتلاوي
Supervisor name: عبد الهادي عباس الابراهيمي | سهاد حميد حسن
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: العكبر (صمغ النحل) Propolis هو نتاج نحل العسل الذي اكتسب شعبية في الطب البديل وذلك بسبب خصائصه الحيوية، وقد استخدم بشكل واسع في الاطعمة الصحية. ان الدراسات المتعلقة بتاثير العكبر العراقي قليلة. ولهذا السبب, فان الهدف من هذا البحث هو تحليل تاثير العكبر الع | Propolis is a honeybee product that has gained popularity in alternative medicine, due to its biological properties and it has been intensively used in health foods. Studies concerning the effect of Iraqi propolis are rare. Therefore, the goal of this work is to analyse the effect of Iraqi propolis on some hematological and biochemical parameters in alloxan - induced diabetic rabbits, as well as study the histological observations of the pancreas, liver and kidney. Female local rabbits (Oryctolagus cuniculus) were used for this study. The overall number of animals used was 30.They were randomly divided into five groups. Diabetes was induced in all rabbits, except normal control, by a single dose of alloxan (150 mg/kg, i.v.). Development of induced diabetes mellitus was confirmed on first week after alloxan administration by examining the fasting glucose level in the blood taken from marginal ear vein. Rabbits with glycaemia were treated with alcoholic extract of propolis for 23 days. Diabetic control group did not treat with propolis. The treated animals were subdivided into three groups according to the dose of propolis extract. Three oral concentrations of propolis extract were investigated (50, 100 and 200 mg/kg/day).The following parameters have been studied were changes in weights of body, liver and kidneys ; changes in the hematological values that include erythrocytes, hemoglobin, leukocytes and hematocrit also changes in the biochemical values which included glucose, total protein, triglycerides, total cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), blood urea, creatinine and Malondialdehyde (MDA), in addition histopathological study of pancreas, liver, and kidneys. The results indicate a significant decrease (P < 0.05) in the body weight of alloxan - induced diabetic rabbits in comparison with control group, while there were significant increases in the weights of liver and kidneys. The present study showed that alloxan induced significant decreases (P<0.05) in all primary blood indices; erythrocytes, hemoglobin, packed cell volume (hematocrit) and leukocytes. Also, serum biochemical changes showed significant increases (P<0.05) in glucose, total protein, triglycerides, total cholesterol, AST, ALT, blood urea, creatinine, and MDA comparison with control group.Histopathological changes in pancreas, liver and kidneys, observed microscopically, revealed degrees of damage in the tissues, while these organs of control groups exhibited a normal architecture. The treatment with alloxan resulted in several forms of histological alterations such as cytoplasmic vacuolation, degeneration, necrosis, cell hypertrophy, nuclear diploid, diffusion of inflammatory cells, dilatation in ducts, stagnation in secretory fluid. in addition, hemorrhage, dilatation and congestion in blood vessels and disorganization of histologic architecture, Generally, the gradual improvement in blood values was noticed with the increase in concentration alcoholic extract of propolis and return back the normal histological shape of pancreas, liver and kidneys. Propolis extract in rabbits had a potent antihyperglycemic effect, antioxidant activities, radical - scavenging capacities, tissue regeneration properties, and that may be due to the high biological activity and nutritive values contents in bee propolis. In conclusion, the results suggest that propolis could potentially contribute for the prevention and treatment of diabetes mellitus.

تاثير تدخين السكائر على فعالية انزيمات AST، ALT وALP ومستويات الشوارد في مصل دم المدخنين == Effect of Cigarette Smoking On The Activity of Serum AST, ALT, ALP And Levels of Electrolytes In Blood Serum of Smokers

Author name: حسام هاشم محمد العزاوي
Supervisor name: حميد محمود مجيد
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: للتدخين اثر مباشر وغير مباشر على معظم اعضاء الجسم، وربما يحدث تاثيره في اقل من ثانية عند استنشاقه ووصوله الى الحويصلات الهوائية وانتشاره في الاوردة الرئوية. ولدراسة التغير الحاصل في : مستويات انزيمات الكبد (AST) Aspartate aminotransferase، Alanine aminotr | Smoking effects directly and indirectly on most organs of the body, this effect may occur in less than a second when inhaled and reach the alveoli and prevalence in the pulmonary veins, to study the change in the : levels of liver enzymes (AST) aspartate aminotransferase, alanine aminotransferase (ALT) and (ALP) alkaline phosphatase and electrolytes (Na +, k +, Cl - , Ca2+, Fe2+ and PO43 - ) in the blood serum of male, Collection of 100 blood samples of male smokers which divided into three groups depending on the smoking period : 1 - first group of 5 - 10 years Comprises 35 smokers, the age range from 25 - 40 years. 2 - second group of 10 - 20 years Comprises 34 smokers, the age range from 25 - 40 years. 3 - third group of more than 20 years Comprises 31 Smokers ages ranged from 25 - 40 years. The above groups Compared with 40 person Non - smoker (group 4), withage range from (25 - 40) year, for the period from November.1st. 2013 to April.1st. 2014, Within the district of Baquba / Province of Diyala.. Results showed : 1 - Increasing in activity of serum liver enzymes AST and ALT (p ?0.01) for a period 5 - 10 years of smoking, and at level of (p?0.001) for both of the periods of smoking 10 - 20 years and more than 20 years as compared to control. While the levels of ALP increased significantly (p?0.01) for three groups as compared with control. 2 - There is no significant differences in the levels of electrolytes Na+, k+, Cl - in blood serum. 3 - There is a significant decrease in the levels of Ca2+ (p?0.001) for the three groups compared to control, while the iron concentration levels increased significantly (p?0.001) in blood serum of the three groups of smokers compared with control. phosphorus levels showed increasing significantly (p?0.05) in both groups of smokers 10 - 20 years and for more than 20 years compared to control. Conclusion from the above : presence of clear effects of smoking on the liver enzymes activity, calcium, iron, phosphorus, while there is no clear effects on electrolytes Na +, k + and Cl - .

دراسة مقارنة بين تاثير سترات الكلوميفين وسترات التاموكسيفين على بعض معايير نطف الفئران == Comparative Study On The Effects of Clomiphene Citrate And Tamoxiphene Citrate On Some Sperm Parameters In Mice

Author name: رائد عبد الاله عباس
Supervisor name: محمد باقر محمد رشاد فخر الدين | محمد عوده سلمان
General topic: Biology
Specific topic: Zoology - Embryology
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: يعد العقم واحدة من المشاكل الاجتماعية المهمة في معظم انحاء العالم.استنادا الى الاحصائيات الحالية حوالي 15% من المتزوجين يواجهون العقم. يؤدي تقييم السائل المنوي غير الطبيعي للاستروجينات الى تشخيص قلة اعداد النطف ونشاطها وشكلها الطبيعي ومن ناحية اخرى تؤثر | Infertility is one of the most critical social problems in the all of population world. According to the current statistics, about 15% of married couples face with infertility. Assessment of abnormal semen parameters leads to diagnosis of oligoasthenoteratozoospermia, and in others, it leads to isolated abnormalities of sperm concentration, motility, and morphology, due to the lack of differentiable or correctable etiology, Both clomiphene citrate and tamoxiphene citrate, as an anti - estrogen, is the most common prescribed medication for male infertility. In humans, ant estrogens interfere with the normal negative feedback of sex steroids at the hypothalamus and pituitary, thereby increasing endogenous gonadotropin releasing hormone (GnRH) secretion from the hypothalamus and follicle stimulating hormone (FSH) and luteinizing hormone (LH) secretion directly from the pituitary. Objective : The objective of this study was to investigate the effect of clomiphene citrate (CC) and tamoxiphene citrate (TC) administered to male mice on quality of sperm parameters.Materials and Methods : Ninety six male mice were divided into two major groups, and every one of these groups was subdivided into three minor groups involving control and two treated groups. In the CC study, 48 mice were administrated orally 0, 0.0125 mg and 0.025 mg as control (G 1), low dose (G 2) and high dose (G 3) for 42 days. While, in the TC study, 48 mice were administrated orally 0, 0.01 mg and 0.02 mg as control (G 1), low dose (G 2) and high dose (G 3) for 42 days. Then, the male mice were sacrificed, both vas deferens were collected and assessment sperm parameters involving sperm concentration, sperm motility and activity, and normal sperm morphology. Results : The results of present study appeared that the use high dose of CC or TC significantly increment (P<0.05) sperm concentration as compared to control groups. However, use high dose of CC significantly increment (P<0.05) percentages of sperm motility and normal sperm morphology. Meanwhile, the percentage of progressive sperm motility was increased significantly (P< 0.05) when low CC dose administered as compared to control and high CC dose groups. In this study, percentages of sperm motility, progressive sperm motility and normal sperm morphology showed best elevation when used low TC dose as compared to both control and high TC dose groups. High TC dose causes significant increment (P<0.05) in the percentage of sperm agglutination when compared to control and low TC dose groups.Conclusions : Based on the results of this study, it can be concluded that the administration of high dose of either CC or TC increases sperm concentration, while administration of low dose of either CC or TC enhances progressive sperm motility.

دراسة بيئية لبعض انواع صنف بطنية القدم الارضية في ثلاث محافظات وسط العراق == An Ecological Study For Some Terrestrial Gastropoda Species In Three Provinces Middle Iraq

Author name: ايمان حسين عبد
Supervisor name: عماد الدين عبد الهادي المختار
General topic: Biology
Specific topic: Environment
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: اجريت الدراسة الحالية للتعرف على انواع صنف بطنية القدم الارضية المتواجدة في بعض محافظات وسط العراق (بغداد, بابل, كربلاء). اذ تم تسجيل الملاحظات الحياتية والتعرف على بيئاتها المفضلة وقياس العوامل البيئية ذات الاهمية. حيث تم تشخيص ثلاثة انواع وهي : Cornu as | The present study was conducted to identify the terresterial gastropoda species located in some provinces of middle Iraq (Baghdad, Babylon and Karbala). They were record Biological observations on their environment and learn about their prefer environments and to measure the important ecological factors. Three species were diagnosed : Cornu aspersum specie belong to Helicidae family, Monacha cantiana and Candidula gigaxii species belong to Hygromiidae family. Monthly sampling was conducted from (October 2013 - July 2014) of the three provincs on two sites per each provinc, Where the samples were collected randomly by five repeaters of one square meter once a month. The study also included soil ecological properties measuring : soil temperature, soil pH, Electrical conductivity, percentage moisture, organic matter content and soil texture.The results showed soil temperature values ranged between (8 - 30) C?, soil pH (7.33 - 7.96), Electrical conductivity (0.7 - 4.1) ds/m, percentage moisture (9 - 33) % , organic matter content (0.81 - 2.88) %. The results showed percentages rate for clay, silt and sand for Jadiriyah (38.39, 49.18, 12.43)%, Zafaraniya (36.66, 51.53, 11.81)%, Alexandria (37.93, 52.54, 9.53)%, Hindyia dam (40.54, 53.05, 6.41)%, Hosseinie (36.28, 45.81, 17.91)% and Twerij (37.79, 50.20, 12.01)%. Generally soil texture clay silt sandy soil for all study sites. The relative abundance of the studied species was recorded, The lowest value for Candidula gigaxii specie was 7.3 % in March in Hindyia dam, while the highest value was recorded for Cornu aspersum specie 75.0 % in December 2013 in Jadiriyah. Rates of population density (individuals / m 2) were calculated for the studied specimens and the results showed the presence of significant differences when comparing the study sites during research period. The population density rates for species the results showed the lowest value for Candidula gigaxii specie 0.4 (individual/m²) in October 2013 in Jadiriyah, and the highest value for Cornu aspersum specie 19.4 (individual/m²) in Janeuary 2014 in Jadiriyah. The results showed that Candidula gigaxii specie is found in sites (Jadiriyah, Zafaraniya, Hindyia dam(along research period, While the results did not record the presence of it in sites (Alexandria, Hosseinie, Twerij).The study showed presence of positive and negative significant relationships between species and soil ecological properties, as it were for Cornu aspersum specie negative significant relationship with air and soil temperature (0.827, 0.763) respectively, positive significant relationship with moisture and organic matter content (0.703, 0.880) respectively, while the results did not record significant differences with pH and Electrical conductivity (0.096, 0.168) respectively. Monacha cantiana specie has recorded negative significant relationship with air and soil temperature (0.431, 0.553) respectively, positive significant relationship with percentage moisture and organic matter content (0.368, 0.550) respectively, while did not record significant differences for pH and Electrical conductivity (0.214, 0.096) respectively. The results showed for Candidula gigaxii specie negative significant relationship of air and soil temperature (0.504, 0.322) respectively, positive significant relationship with moisture and organic matter content (0.380, 0.569) respectively, While did not record significant differences with pH and Electrical conductivity (0.088, 0.170) respectively.

تقييم مستوى هرموني الجوع والسمنة لدى مرضى فرط ضغط الدم في محافظة النجف الاشرف == Assessment of Obestatin And Ghrelin Levels In Hypertensive Patients In Al - Najaf Al - Ashraf Governorate

Author name: زهراء محمد فاخر عطية النفاخ
Supervisor name: ارشد نوري غني الدجيلي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: تهدف هذه الدراسة الى تقييم مستوى الهرمونات المكتشفة حديثا هرمون الجوع ?هرمون السمنةونسبة هرمون الجوع الى هرمون السمنة في مرضى ضغط الدم لمعرفة العلاقة المحتملة بين مستوى هرمون جريلين, اوبستاتين مع عوامل مختلفة بما في ذلك الضغط الانقباضي, الضغط الانبساطي و| The present study aims to assess the newly discovered ghrelin, obestatin hormones levels and ghrelin to obestatin ratio in patients with hypertension disease and to find out a possible relationship between obestatin, ghrelin level with different criteria including, systolic blood pressure, diastolic blood pressure and body mass index (BMI) in patients with Hypertension disease and compared with healthy controls as a tool for monitoring and even possibly prediction or diagnosis of these diseases. 69patients are diagnosed with hypertension disease (33males and 36 females) and 20 control healthy subjects (10 males and 10 females) having no history of diabetes mellitus, renal failure and other disease enrolled in this study.The patients are selected from both sexes (males and females) their ages ranged between (40 - 70) years old. Patients with hypertension disease are recruited from AL - sader teaching city in AL - Najaf AL - Ashraf Governorate during the period from September to December 2013. Results of the present study in general revealed that there is high obestatin level in patients with hypertension diseases in a comparison with control group, low ghrelin level in patients with hypertension disease comparing with control group and ratio of ghrelin to obestatin ratio was decreased in patients undergo from hypertension when compared with control group.The result showed a positive correlation among obestatin, systolic pressure, diastolic pressure and body mass index (BMI) with negative correlation between gherlin, systolic pressure, diastolic pressure, and body mass index (BMI) and negative correlation between ghrelin to obestatin ratio with blood pressure. The present study concluded that ghrelin to obestatin ratio might play role in blood pressure regulation

دراسة بيئية للهائمات النباتية في مبزل CD4A (مبزل هور شعيب) الديوانية - العراق == Ecological Study of The Phytoplankton In Cd4A (Hor Shuaib) Drainage In Al - Diwaniya Iraq

Author name: ابتسام كاظم خضر الصالحي
Supervisor name: فؤاد منحر علكم
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: تضمنت الدراسة الحالية بيئة الهائمات النباتية في مبزل CD4A (مبزل هور شعيب) في الديوانية - العراق. للمدة من ايلول 2011 حتى نهاية اب 2012 حيث تم اختيار ثلاثة مواقع على المبزل، يقع الموقع الاول في بداية مجرى المبزل عند قرية شويخة ويقع الثاني على بعد 10 كم من | The present study deal with phytoplankton in CD4A drainage for a year starting from September 2011 to August 2012.Three sites have been chosen, the first is near shoekha village at the beginning of the drainage, Second is after the first site ten kilometers, while the third site is at the end of the drainage after Neffr sub district. The study includes measuring physical, chemical and biological properties of drainage water which includes studying quality and quantity of phytoplankton. The study shows that the temperature of the air is ranged from 12.5 - 42.1 c? and water temperature is about(11.5 - 31.5) c? light penetration from 16 - 57cm while turbidity is(49 - 2.1)NTU The study shows that the pH is limited in along the period of the study (6.4 - 8.4), Electrical conductivity is approximately about (2247 - 8030 µs/cm), the water drainage Oligohaline The dissolved oxygen values was from 3 - 8.6 mg/l and carbon dioxide values were ranged from 80.2 - 227.8 mg/l, it also show that drain water is alkaline and bicarbonate ions prevailed, total alkaline is (281.3 - 102.6)mg/l of calcium carbonate. It is discovered that the water in the area under investigation is too hard, hardness values were ranged from 1439.5 - 517.6 mg/l, while Calcium concentrations were ranged from 232.5 - 98.1mg/l andMagnesium concentrations were ranged from 220.3 - 64.2mg/l. Chloride concentrations were ranged from802.01 - 334.1µg/l, while Sulfate concentrations were ranged from 1184.9 - 421.03µg/l Plant nutrients Nitrite concentrations are about (6.22 - 0.58) µg/l, Nitrate concentrations (122.3 - 19.5) µg/l and Phosphate concentrations are about (2.09 - 0.1) µg/l, Phytoplankton types which are diagnosed during study period (143). Bacillariophyceae is dominate on other class and Chlorophyceae Cyanophyceae, Euglenophyceae, the study shows there are99, 120 and 125 species in the first, second and third site, the species that have highs number (Nitzschia, Navicula, Cynbella, Oscillatoria, chlorella, cocconeis, Amphora.Cyclotella), During the period study there are seasonal and site changes is total number of of phytoplankton which ranged about)5218.9 - 267.9 (x 103 cell/cm2. Statistical analysis showed there morale differences between locations and seasons in relation to physical, chemical and biological features also there is an interference between months, there are negative and positive, morale and in morale correlation between phytoplankton and physical, There are negative and positive, morale correlations between phytoplankton and physical, chemical features that measured during study period at probability level of (0.05).

دراسة بيئية ونوعية لمشروع تصفية ماء الكرخ في الطارمية == An Ecological And Qualitativ Study For Al - Karkh Drinking Water Plane At Al - Tarmia

Author name: احمد توفيق احمد المشهداني
Supervisor name: رياض عباس عبد الجبار
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: نظرا لما لمياه الشرب من اهمية كبيرة في حياة الانسان وضرورة ان تكون هذه المياه ضمن مواصفات ومعايير خاصة, فضلا عن ظهور العديد من مشاكل تلوث مياه الشرب في مختلف مدن العراق. دعت الحاجة لاجراء هذه الدراسة لبيان مدى صلاحية مياه الشرب المنتجة من مشروع تصفية ماء | Owing to the drinking water of great importance in human life and it is important to be under the specifications and standards, as well as the emergence of many of the problems of contamination of drinking water in various cities of Iraq. Needed to conduct this study to demonstrate the viability of drinking water produced from the project filter water Karkh (which is the largest project the liquidation of water in the Middle East, it is feeds many of the cities of Baghdad, on the side of Karkh) and compare the specifications of Iraq and the global standard for drinking water in order to investigate the potential weaknesses in the project and study the possibility of the development of such large projects as a first step to improving the situation of drinking water in Iraq. So were the tests, physical, chemical and biological common to drinking water in the project and divided into four main stations, namely, (station socket raw water, sedimentation basins secondary sand filters, pumps, distribution of drinking water), including nine stations secondary starting of the month of October - 2010 to July - 2011.The results showed high values of turbidity, and electrical condactivety and pH, and dissolved oxygen, and biological dimond of oxygen, and alkalinity, and total hardness, calcium hardness, magnesium hardness, chloride, phosphate, nitrite, silica, fluoride, sodium, potassium, and heavy metals, namely, (iron, copper and cadmium, nickel, lead, chromium and zinc), and the total numbers of bacteria, and total coliform bacteria, the fecal coliform bacteria and E. Coli in drinking water for most of the stations in the Alsta spring and low concentrations in summer and autumn.Values for water temperatures between (4 - 37.5) c, as recorded differences significant between the months of winter and spring, summer and autumn, and the values of brownish discharge ranged between (250 - 0.3) NTU as it showed the differences of the moral high values of turbidity in the winter and fall in the summer and fall in each stage of the treatmant with it, and the values of electrical conductivity was between (520 - 895) Micro Siemens / cm 3. The water Alnehrualcherb in the project was registered as a weak base pH values between (9.7 to 1.7), and it was well ventilated, as the oxygen values were high in the winter months registered clear monthly variation ranging between (5.14 - 2.3) mg / L, either values biologicaldimond of oxygen were values between (4.4 - ND) mg / L and found that basal total stations ranged from every stage of the liquidation potential in increasing concentrations of dissolved oxygen, between (160 - 100) mg / L, and found that drinking water hardness too, as that the values of total hardness ranged between ( - 215 335) mg / L as suited their values directly proportional to both the calcium and magnesium hardness ranging between (197 - 139) and (31.472 - 16.128) mg / L, respectively, while for the values of the chloride ranged between (69 - 25) mg / l, while the fluoride has recorded values ranged between (15 - 0.05) mg / L, with values ranging between sulfate (169.2 - 90) mg / L, free chlorine residual values ranged between (4.18 - 1.1) mg / L, and materials for suspended solids ranged values between (84 - ND) mg / L proven contribution stages of liquidation, all in the rates of removal of different, either TDS fluctuated values between (545 - 315) mg / L, with log Aluminum values ranged between (0.98 - 0.01) mg / L, but for the potassium values ranged between (3.2 - 2.15) mg / L, and sodium values ranging between (6.31 to 3.21) mg / liter.As for the concentrations of heavy metals did not detect any significant concentration of heavy metals measured except for iron ranging between concentrations (9.2 to 8.1) mg / L, so after the industrial plants that contain the droppings of these metals. The nutrient values were recorded between nitrite (0.024 - 0.001) mg / Trosgelt nitrate values between (1.7 - 0.16) mg / l, recorded between ammonia (0.54 - ND) mg / L, placed at either phosphate values ranged between (0.26 - ND) mg phosphorus atom - phosphate / L, while for the values of silica fluctuated between values (5.12 to 5.1) mg atom of silicon - silica / liter. As for the study of signs bacteriological ranged the total numbers of bacteria in air through (24) hours between (555 - 0) × 10 cells / 1 ml, and ranged in numbers for a period of (48) hours between (1186 - 0) 10 × cells / 1 ml, while for the numbers of total coliform bacteria fluctuated between (1700 - 0) cells / 100 liters, and fecal coliform bacteria, they ranged (1100 - 0) cells / 100 ml, and the E.coli bacteria, the numbers fluctuated between (1100 - 0) cells / 100 ml. For to match the drinking waterproduced from the projects liquefaction research found matching the values and concentrations of brownish, and connectivity electrical and pH, and dissolved oxygen, and the requirement is vital for oxygen, and basic college, and chloride, and ten college, and magnesium, nitrite, phosphate, and the total numbers of bacteria, and the numbers of total coliform bacteria, and fecal coliform bacteria and E Coli, and silica with the standard specifications for drinking water the Iraqi and global at all stations.While the study results show non - conformity of calcium concentrations in this study with the standard specifications for drinking water of Iraq and the world.

عزل وتشخيص البكتيريا Bacillus thuringiensis ودراسة تاثيرها في بعض المقاييس الحياتية لذبابة ثمار القرعيات (Diptera : Tephritidae) Dacus ciliatus == Isolation And Identification of Bacillus Thuringiensis And Studying Its Effects On Some Biological Parameters For Dacus Ciliatus (Diptera : Tephritidae)

Author name: ادريس صالح جليل
Supervisor name: عدنان نعمة عبد الرضا العزاوي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: تضمنت هذه الدراسة عزل البكتيريا Bacillus thuringiensis من مصادر بيئية مختلفة في محافظة ديالى وتشخيصها بالطرق الكيموحيويه والوراثية والجزئية، اذ جمعت 51 عينة من ترب مختلفة وهي(زراعية وحديقة وغير زراعية) وكذلك من الماء، من خمسة اقضية في محافظة ديالى (بعقوبة | This study included the isolation of pathogenic bacteria Bacillus thuringiensis from different environmental sources in Diyala province, diagnosis biochemical and genetic partial ways, Mar 50 samples collected from different soils, namely, (agricultural soils and garden soils and non - agricultural soils) as well as from the water, from five districts in the province of Diyala (Baquba, Khalis, Muqdadiyah, Khanaqin, Balad Ruz) for the period from 1 \ 9 \ 2014 to 20 \ 11 \ 2014. Showed the results of biochemical diagnosis and the presence of four samples and by percent 8% It was of a negative for transplantation bacterial and 46 sample growth by percent 92% It was of a growth positive for transplantation bacterial and found that 41 samples of which by percent 82% belong to the genus Bacillus and the species of Bacillus thuringiensis and the percentage of bacteria isolated of agricultural soils 94% and soils garden 93% and water 75%, and soils non - agricultural 44%. The pathogenic bacteria Bacillus thuringiensis of positive bacteria a gram dye and spore forming and The most of the natural habitat this bacterium is soil and it is capable of producing the diversified varieties of crystalline proteins with insecticide property. All isolates were tested for this bacteria that which were able growth on the media selective Acetate Sodium Selective and the production of insecticidal crystalline proteins (ICP) When staining by dye Coomassie brilliant blue (CBB) to conduct genetic partial diagnosis using several extraction (Mini gDNA Bacteria kit) processed by Bioneer company was the purity of DNA extracted ranging between (20.1 - 1.22) for all selected isolates, have been diagnosed with the bacteria at the level of genus and species Using the gene 16S rDNA with a molecular weight of 380 base pairs where the results showed polymerase chain reaction (PCR) that all isolates contain this gene and the same molecular weight which is 380 base pairs, also was diagnosed isolates containing the gene component crystalline proteins which cry a e molecular weight 200 base pairs using primer a specialist for this gene in polymerase chain reaction showed the results of gene doubled that all isolates contained the gene and the same molecular weight of 200 base pairs. After making sure of pathogenic bacteria as belonging to the genus Bacillus and species Bacillus thuringiensis according to their chemical diagnostics biochemical and diagnostic genetic partial, attended different concentrations They (1, 2, 3, 4, 5) g/L of thebio preparation for these bacteria to study their impact on the different roles of Cucurbits fruit fly Daus ciliatus. Has been tested the effect of different concentrations of bio - preparation of these bacteria isolated from soil and water on the roles of this insect which eggs and larvae and pupa and AdultsThe results of test the effect of bio - preparation of these bacteria on the eggs of age (1 - 2) Since the highest rate of hatchling (3.2) and by (89%) at a concentration of 1 g / l of bio - preparation and lowest hatchling (0.6) and by (19%) at a concentration of 5 g / L of bio - preparation comparison with control (3.2) and by (96%) for the isolated bacteria from soil Since the highest rate of hatchling (3.1) and by (92%) at a concentration 1 g / L of bio - preparation and lowest hatchling (2.1) and by (62%) at a concentration of 5, 4 g / L of bio - preparation comparison with control (3.2) and by (96%) for isolated bacteria from water, and on The last larval phase, The highest the value of the rate of highest (2.0) and by (99%) at a concentration of 5 g / L of bio - preparation and The less the value of the rate of loss (0.5) and by (26%) at 1g / L of bio - preparation concentration compared with the control treatment (0.07) and by (%3) for isolated bacteria from soil.reached its highest value the loss rate (1.2) and by (57%) at a concentration of 5 g / L of bio - preparation and less the value of the loss rate (1.0) and by (6%) at a concentration 1 g / L of bio - preparation comparison with control (0.07) and by (3%) for the bacteria isolated from water and on pupa age(6 - 7) day, reaching the highest rate of emergence of pupa (3.2) and by (92%) at a concentration of 1 g / L of bio - preparation and less the emergence of pupa rate (0.4) and (13%) at a concentration of 5 g /L of bio - preparation comparison with control (3.2) and by 96% for bacteria isolated from soil reaching the top of the emergence of pupa rate (3.1) and by (92%) at a concentration of 1 g / L of bio - preparation and less the emergence of pupa rate (2.1) and by (62%) at a concentration of 5 g / L of bio - preparation comparison with control (3.2) and by (96%) for bacteria isolated from water. and on kill adult, The highest killing rate for males (1.6) and by (32%) and females (1.5) and (30%) at a concentration of 5 g / l of bio - preparation and less killing of the male rate (0.5) and (10%) and females (0.4) and by (13%), comparison with control males (0.1) and by (2%) and females (0.1) and by (2%) for the isolated bacteria from soil. The highest kill the male rate (0.8) and by (32%) and females (0.7) and (30%) at a concentration of 5 g / L of bio - preparation, and less killing of the male rate (0.2) and (4%) and females (0.4) and by (13%) at a concentration of 1 g / L of bio - preparation comparison with control males (0.1) and by (2%) and females (0.1) and by (2%)for the isolated bacteria from water and one female productivity of egg reached its highest production rate eggs 84.5 eggs per female at a concentration of 1 g / L of bio - preparation, and less egg production rate of 54.5 eggs per female at a concentration of 5 g / L of bio - preparation comparison with control eggs productivity rate of 87.5 egg female for isolated bacteria from soil reaching its highest egg production rate (85%) eggs per female at a concentration of 1 g / L of bio - preparation, and less egg production rate (79.5%) eggs per female at a concentration of 5 g / L of bio - preparation, compared with treatment control productivity rate of whites (85.5%) eggs per female for isolated bacteria from the water

تقييم فعالية مستخلصات مستنبت القمح Triticum aestivum في بعض الانواع البكتيرية المسببة لاخماج مختلفة == Evaluation The Activity of Germinated Wheat Extractions Triticum Aestivum On Some Bacterial Species That Cause Different Infections

Author name: اسماء عيسى محمود
Supervisor name: رشيد حميد حسن
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: The study had included isolation and diagnosis of three hundred samples for both sexes their ages were between (1 - 60) years of the inpatients and outpatients in Tikrit Teaching Hospital and General Samarraa Hospital during the period from 1/9 / 2011 to 1/3/2012. The infections were distributed among the burns infections, the wounds, urinary tract infections (UTI) and female genital tract infections (High vagina). This study clarified that (192) sample from the total samples showed bacterial growth in percentage 64%. The results of this study indicated to the proportion 58% with (UTI) by (87) bacterial isolation were produced from (150) isolation. The percentage of burns infections were 81.81% from (55) sample which distributed among the first, second and third class burns. The burns of second and third class gave bacterial growth in a proportion 100% while the first class burns gave 64.28%. Concerning the wounds infections, bacterial growth was 62.9% from (62) sample. The infections proportion of (H.V) was 63.63% from (33) sample. The results of the current study showed that the infections in females were higher that in males with respect to (UTI), while there was not considered difference between the males and females about the burns and wounds infections. Differently, the (H.V) infections were just for the females in the ages between (18 - 45). It was clear according to the results of diagnosis that Escherichia coli had the highest proportion of isolations 25.52% from the total isolates followed by Staphylococcus aureus 18.23% Pseudomonas aeruginosa 15.63%, both Klebsiella pneumoniae and Proteus miribilis 10.42% Staphylococcus epidermidis 4.69% Citrobacter freundii 4.17%, Staphylococcus saprophyticus 3.64% both Serratia marcescens and Enterococcus faecalis 2.6%, Streptococcus pyogenes 1.56% and Staphylococcus intermedius had the least percentage 0.5% by one from (192) isolate. The sensitivity of these isolates was tested by (10) antibiotic which included quinolones, aminoglycosides, beta - lactams and tetracyclines antibiotics. Among these antibiotics imipenem and ciprofloxacin were more effective, while the other antibiotics were different either active or not active at all.The wheat germinating was used in this study with the ages (36 - 48) hour and its activity was tested by using some bacterial kinds. After completing several quantitative and qualitative tests on this germinating, the carbohydrates proportion was between 67 - 70%, the protein proportion by folin method was between 10 - 8%, the lipids proportion was 1.5%.Some mineral elements in germinated wheat were estimated which include Calcium, Iron, Magnesium, Potassium, Copper, Manganese, Zinc, Selenium, and Sodium element, active materials were also estimated in the wheat germinating extracts which had inhibitory effect on the microbiology. It was clear by the tests that the germinating contains a lot of Phenolic compounds, Terpeniods, glycosides and steroids. These materials were extracted by using some extracts included the cold water extracts and hot water extracts, alcoholic extracts (ethanol and methanol) and phenolic extract. All the extracts prepared in concentrations (25, 50, 100, 200) mg/ ml. This study showed that the phenolic extract had high effect on the bacterial kinds in comparison with the methanolic and ethanolic extracts at (200) mg/ ml concentration while the cold water extract had less effect on these bacterial isolates in comparison with the above extracts, finally the hot water extract had no inhibitory effect against any isolated bacterial type with all the concentrations. The inhibitory action of these extracts was compared with some antibiotics as a control sample. These antibiotics represents different groups of antibiotics and which contained Imipenem, Ciprofloxacin, Gentamicin, Ceftazidime in addition to Augmentin.In this study the minimum inhibitory concentration (MIC) of the fenolic, alcoholic and watery extracts was determined and the concentration was different according to the extract type, the bacterial type and the isolation site.

التحري عن مؤشرات وراثية وفسلجية لتحمل الجفاف لصنفين من الحنطة Triticum aestivum L. == Investigation of Genetic And Physiological Markers For Drought Tolerance of Two Cultivars of Wheat (Triticum Aestivum L)

Author name: اشواق ابراهيم بشير العبيدي
Supervisor name: شاكر مهدي صالح | عقيل حسين العاصي
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تم في هذه الدراسة البحث عن امكانية ايجاد مؤشرات وراثية ترتبط بمؤشرات فسلجية وذلك من خلال الدراسة على مستويين : ا - المستوى الفسلجي : زراعة صنفين من الحنطة الناعمةL Triticum aestivum في حقول كلية الزراعة/ جامعة تكريت لموسم النمو 2010 - 2011، وذلك بزراعة بذو | This study was conducted to investigate a genetic marker linked to physiological marker through a design an outdoor experiment which includes two parts : - 1 - Physiological part : Included planting two cultivars of soft wheat (Triticum aestivum L.) in the fields of the college of Agriculture, university of Tikrit, during growth season (2010 - 2011). The stage of work had been done by cultivated the seeds of two cultivars of wheat (Sham - 6, Abu - Ghraib) in pots, when they reached the stage of (4 - 5) leaves it had been exposed to two dryness periods 7 and 10 days. It spread with growth regulator Indole Acetic Acid (IAA) (150 ppm) to know which type is more resistance for dryness through estimation of proline concentration as a physiological marker and to indicate the effect of IAA to reduce the dryness effect in some features of growth physiological function and yield component according to complete Randomize Design (CRD). Some markers of drought tolerance have been estimated including : proline content, chlorophyll concentration and the degree of membrane stability then plants were left for harvesting period. The results of the outdoor experiment were : 1 - Exposure the plants to dehydration periods during their growth caused reducing of the most studied characters (leaf area, plant height, tillers number, concentration of chlorophyll A and B and the total chlorophyll), while there was an increase in the content of the proline and the injury index of plasma membrane. 2 - Some characteristic did not showed effect by drought periods (dry weight of plant, number of days of flowering, spikes number, spike length, number of grains/ spike and weight of 1000 grains).3 - Sham - 6 cultivar was superior than Abu Ghraib - 3 in three characteristic (number of days of flowering, leaf area, weight of 1000 grains), while Abu Ghraib - 3 cultivar was superior in the grains number /spike than sham - 6 cultivar.4 - The concentration of IAA (150 ppm) had a different effect. It had a positive effect in the decrease drought for some characteristic (proline content, injury index of plasma membrane, leaf area, plant height, dry weight, spike number and grains yield).2 - Genetic part : DNA has been isolated from fresh leaves of two types of wheat, arrange yields of DNA were in the range of (40 - 150) micro gram per 1.5 gram of plant leaves, and the purity ranged between (1.2 - 1.7). The reaction PCR was done by using 11 primer of the Random Amplified Polymorphism DNA (RAPD) in addition to two pair of specific primer to detect the genes which are responsible for coding of two enzymes which involved in anabolism and catabolism of proline. The results obtained are as follows : - 1 - Five primer of RAPD did not appear any binding site (OPA - 18, OPL - 20, OPA - 11, OPQ - 17 and OPH - 14).2 - Six primer of RAPD have appeared main and monomorphic bands (OPO - 16, OPH - 07, OPN - 16, OPM - 20, OPJ - 13 and OPQ - 06), in addition to the absent of the other bands from some treatments. 3 - The two pair of specific primer have given three monomorphic bands with different molecules weight.4 - The primer (OPH - 07) has appeared a unique band with molecular weight (1670) pb for Sham - 6 cultivar during second drought period (10 days without hormone). These results obtained from this study were identical with physiological part in the ability of both types to drought tolerance

وظائف الغدة الدرقية وبروتين C التفاعلي لدى مرضى العجز الكلوي المزمن المستمرين على الديلزة الدموية ومرضى الزرع الكلوي == Thyroid Functions And C - Reactive Protein In Chronic Renal Failure Patients On Hemodialysis And Kidney Transplantation

Author name: احمد جاسم محمد المشهداني
Supervisor name: نبيل خالد محمد علي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: يعاني مرضى الغسيل الدموي من امراض مزمنة يعتقد بانها لاتطال الغدة الدرقية, ولتقييم ذلك من خلال كل من الاداء الوظيفي الكلوي والدرقي لدى مرضى الفشل الكلوي المزمن والزرع الكلوي تم : - 1) قياس مستويات اليوريا والكرياتنين والفسفور وبروتين C التفاعلي, الكالسيوم | Patients on regular hemodialysis suffer from a chronic illness that is believed not to involve the thyroid gland. In this study, we evaluated the thyroid and kidney functions in Patients with end - stage renal disease and transplantation from : - 1) measurment levels of urea, creatinine, phosphorus, and C - reactive protein, calcium and albumin in patients and compared control. 2) measurment levels of thyroid hormones fT4, fT3 addition to thyroid stimulating - hormone TSH in the blood serum of patients and compared control. This study was conducted in Baquba teaching Hospital /Department of dialysis, the period from November.1st. 2013 to April.1st. 2014, from which to collect 80 blood samples, 40 samples hemodialysis Patients including (26 males, 14 females), 40 person Renal or kidney transplant Patients (32 males, 8 females) do not suffer from thyroid disease, compared with 40 blood samples for healthy people(control) (32 males, 8 females), within the age range of (20 - 70 years).. The Results of the study revealed a significant increase of probability (p<0.001) in levels of urea, creatinine and phosphorus in hemodialysis patients compared to control (21.932 ±1.072, 4.766 ± 0.122 mmol/L) (413.400 ±29.109, 62.375± 1.142 umol/L) (5.397 ±0.370, 3.875 ± 0.127 mg/dl) respectively. the results also indicate revealed a rise in the concentration of acute phase protein (C - reactive protein) by (50%) in hemodialysis patients, which reflected negatively increase the mortality rate in these patients, and low in glomerular filtration rate in hemodialysis patients compared to control (16.152±1.395, 126.325 ±2.020 ml/min) respectively. while still this significant difference in these levels with the exception of urea and glomerular filtration rate, which continued at the same level in renal transplant patients. The results indicated the presence of a significant decrease of probability (p<0.001) in the levels of calcium and albumin in hemodialysis patients compared to the control (7.857± 0.266, 9.058 ± 0.063 mg/dl) (3.333±0.156, 4.146 ± 0.108 g/dl) respectively. This decrease to become less significant in renal transplantation patients (P<0.01). The Thyroid hormone levels were the results of the statistical analysis show a significant decrease of probability (P <0.001) in Thyroid hormone levels Free triiodothyronine in hemodialysis and kidney transplant patients as compared to the control (3.233±0.151, 4.519 ± 0.100, 5.191 ± 0.137 pmol/l) respectively. While Free Thyroxine levels did not show a significant difference at the hemodialysis group compared to control , while the renal transplant group the results indicated the presence of a significant increase of probability (P <0.01) in hormone levels as compared to control amounted to (16.400 ± 0.778, 14.671± 0.401 pmol/l ) respectively. did not significant affected variations of the thyroid - stimulating hormone among the two groups of patients compared to the control. Notes from the above results presence decrease in thyroid hormone levels Free triiodothyronine fT3 in patients with hemodialysis and kidney transplant.

دراسة تاثير مستخلصات نباتي القرنفل والخروع على نمو بعض الفطريات المنتجة لانزيم البروتييز والمسببة لالتهاب الاذن الخارجية == Study Effect of Syzigium Aromaticum And Ricinus Communis Extracts On Growth of Some Fungi That Produce Protease And Cause Otitis Externa

Author name: بتول عمران ذيب
Supervisor name: خليل ابراهيم بندر النعيمي | ذكرى احمد حمادة
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: ضمنت الدراسة عزل وتشخيص الفطريات من الاذن الخارجية في مستشفى تكريت التعليمي - وحدة الاذن والانف والحنجرة والعيادات الخاصة في مدينة تكريت, خلال الاشهر تموز, اب وايلول 2008. جمعت 100 مسحة اذنية من المرضى المشخصة اصابتهم بالتهاب الاذن الخارجية الفطري من قبل | The study was included isolation and identification of fungi from the external ear in Tikrit teaching hospital, the unit of Ear, Nose, and throat (E.N.T.) and Privete clinics in the city of Tikrit during the months July, august, and September2008. 100 ear swabs were collected from the patiants whose diagnosed by Otologists with Otomycosis, and 50 ear swaps were collected from healthy persons in the same city and period time, These samples were examined by dierect microscopic examination treated with KOH 10% with hot flame to ensure the fungal infections, and Culturing on Sabouraud, s Dextrose Agar was done for isolation and identification of types of fungi which responseple for infection, The result of direct microscopic examination and culture showed that 76(76%) were positive for fungi, 64 were positive by direct microscopic examination and culture, and 12 posative in culture only. The most common fungi isolated from patient were Aspergillus Spp.36). (%22 followed by Candida Spp. (%67. 10), Trichophyton Spp(%6.5) , and Penicillium spp. (%3.94%), Aspergills niger appeared as the predominant fungi (%84. 36) followed by Aspergillus flavus (%36. 22), Candida albicans (42. 18%), Aspergills terrous (7.89%) , Candida kruzi, Penicillium spp (3.94%), Trichophyton mentagrophyte, Trichophyton verrucosum (63. 2%) and Trichophyton scheonlieni (%1.31), and no fungi were isolate from the healthy person. Higher percentage of fungi infection were recorded in female 46(60.52%) than male 30(39.47%). and the higher percentage for fungal infection was recorded in the age group (31 - 40) with percentage (23.68%). fungal ear infection was recorded with highest percentage in right ear 47 (61.84%), and for the left ear 29 (38.15%). and high rate of infection was recorded in august (73.69%), Higher percentage of infection was recorded in patient house wives (52.36%), the itching was the most commen symptom , The most habits used to clean the ear by dearty body (51.31%), Ahigher percentage (76.4%) recorded in non educated persons. The results of active ingredients test for Syzigium aromaticum and Ricinus communis revealed that clove dry flower buds contain Flavonoides, Glycosides, Tannins, Volatile oils, Terpenes, Resins, and Phenols, while castor leaves contain Flavonoids, Tannins, Volatile oils, Terpenes, Resins, Saponins and Phenols, pH of clove bud 4.3 and pH of castor leaves 5.8. Aqueous and alcoholic extracts were Prepeared, which belongs to Syzigium aromaticum and Ricinus communis under the study, commercial oil for clove and seed castor were used in the study, the effects of inhibition of Aqueous and Alcoholic extracts and commercial oil had been studied on the growth and protease production on the three fungi which were showed the high ratio appeared among the isolation sample under the study with 5 concentration 10, 20, 30, 40, 50 (mg /ml), and the affected rate of these extracts and oils under the study on fungi were varied. The relation between Aqueous extract of clove bud and fungi growing was increasing relation, While Alcoholic Extract and commercial oil showed preference in the inhibition of the isolated fungi grown while its effect on Candida albicans was increase with increase the concentration, The effect of aqueous and Alcoholic Extract of castor leaves on Moulds showed reverse effect. on the other hand the commercial oil effect was slight effect, and both extracts did not showed effect on the Candida albicans as well as the Commercial oil.When adding Clove Extracts and Commercial oil and in all concentrations dose not gave any production of the Protease enzyme in the solid media, whereas the extracts of castor leaves and commercial oil were showed effects on the production of Protease enzyme, and Aspergills niger was more effected and these extracts did not showed an effect on the Candida albicans.Aqueous and Alcoholic extract and commercial oil to the Clove revealed higher influence rate than the effect of the Castor leaves extract , and in a different rate

تحديد تعبير الجين المتحمل للملوحة TaGSK1 في عدد من اصناف الحنـطـة == Determination of Gene Expression of Salt Tolerant Gene Tagsk1 In Wheat Cultivars

Author name: ايمان نعمان اسماعيل
Supervisor name: نورية عبد الحسين علي | مجيد ارشيد سباح
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
University: University of Baghdad
Language: Arabic
University location: Baghdad
First pages:
Abstract: نفذت الدراسة الحالية في مركز بحوث التقنيات الاحيائية/ جامعة النهرين للمدة 2011 - 2012 لغرض الكشف عن جين الملوحة TaGSK1 ودراسة التعبير الجيني له في صنفين من الحنطة فرات ودجلة والتركيب الوراثي 2H المنتخبة لصفة تحمل الملوحة مقارنة مع الصنف تموز2 الحساس للملو | This study was conducted in biotechnology research center, Al - Nahrain university in 2011 - 2012 to detect the gene responsible for salt tolerant (TaGSKI) and study its expression in two wheat (Tigris and Euphrates) cultivars and the genotype (2H) were selected for salt tolerance through improvement and breeding programs as compared with to the sensitive local wheat cultivar (Tamooze 2). Seeds of the these cultivars were sown in flasks that contained Ms media in three salt levels (0, 15, 25) ds/m with three replication for each. Five seeds from each cultivars were sown in each replicate. After 10 - 15 days from the sowing, the percentage of germination was estimated, and samples of leaves were collected for RNA extraction and then changed to the cDNA. B - actin gene that consider endogenous control and salt tolerant gene TaGSK1 were amplificated by using PCR technique to discover the gene and by QPCR to estimate gene expression by determine the CT (Cycle Threshold) value for B - actin and TaGSK1 genes in wheat plants of the studying cultivars. Number of DNA dilutions of 189bp molecular weight that extracted from agarose gel were used to determing CT value for each dilution. Standard curves were drawn to find out value of PCR Efficiency which was used for gene expression for the salt tolerant gene TaGSK1.The results of germination percentage (%) showed that there were high differences between the two salt tolerant cultivars (Tigris and Euphrates) and 2H genotype and local cultivar (Tamooze 2). Euphrates cultivar gave 100% germination percentage, and the Tigris cultivar and 2H genotype gave 89, 86% germination percentage respectively at 25ds/m. whilst the local cultivar gave zero germination percentage at the same level of salinity. At 15ds/m level, also the Euphrates cultivar gave 100% germination percentage while the Tigris cultivar and 2H genotype gave 96, 94% germination percentage respectively as compared to Tamooze cultivar that gave 13% germination percentage. All the cultivars have 100% germination percentage at 0.0ds/m level. The conclusion of this result is the two cultivars (Tigris and Euphrates) and 2H genotype more salt tolerance than local cultivar at this growth stage which more salt sensitive than others growth stages. The results of PCR reaction were also indicated that Tigris and Euphrates cultivars and 2H genotype have salt tolerant gene TaGSK1, while this gene did not exist in the local cultivar. There were two bands of TaGSK1 gene that have 189bp and 404bp molecular weight in the Tigris and Euphrates cultivar and the genotype 2H, while the local cultivars have only one band that have 404bp molecular weight. These results were indicated that 189bp molecular weight of this gene is responsible for salt tolerance character in these cultivars.The results of QPCR reaction also were revealed that there is difference between the cultivars in their gene expression. Tigris and Euphrates cultivars and 2H genotype gave the highest gene expression at 15ds/m and increased at 25ds/m as compared to 0.0ds/m. At the second level 15ds/m the gene expression of the two cultivars and genotype was 0.8682, 0.8190 and 0.8688 respectively, and at the third level 25ds/m was 1.656, 1.3176 and 1.2665 respectively, while at the first level 0.0ds/m was less than the other 15, 25 ds/m as compared to local cultivar that the gene salt tolerant (TaGSK1) have no gene expression at the same salt levels. This result indicated that the local cultivar does not have salt tolerant gene TaGSK1. From these results we can revealed that TaGSK1 gene was found in the Tigris and Euphrates cultivars and 2H genotype, and this gene can be considered from high salt tolerant gene, because it gave the highest gene expression at the highest salt level 25ds/m. Therefore this gene help the plant to tolerate salt stress and grow very well. The results also showed that the two salt tolerant cultivars (Tigris and Euphrates) and the salt tolerant genotype (2H) proximately have the same salt tolerance degree, so they have proximately the same gene expression as compared to salt sensitive local cultivar which have no salt tolerant gene TaGSK1. This gene is good indicator for salt tolerance at high salinity levels 15, 25 ds/m in these cultivars and genotype.

التاثيرات المرضية النسجية لعقار ليفيتيراسيتام في التكوين الجنيني لكلى اجنة ومواليد وحوامل الجرذ الابيضAlbino rats (Rattus rattus) == Histopathological Effect of Levetiracetam Drug On The Kidney Development In Rats Embryo, Newborns And Pregnant Albino Rat (Rattus Rattus)

Author name: لمياء هادي محمد الابراهيمي
Supervisor name: نهلة عبد الرضا البكري
General topic: Biology
Specific topic: Zoology - Embryology
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: الصرع هو حالة مزمنة تتميز بنوبات متكررة دون سابق انذار، سببها خلل في نقل الاشارات الكهربائية داخل الدماغ، وعقار Levetiracetam (LEV) من العقاقير المضادة للصرع، ذو خصائص دوائية ملائمة وتظهر فعاليته في تحسين السيطرة على النوبات، ويكون امتصاصه في البلازما سري | Epilepsy is a condition characterized by recurrent abrupt seizures episodes caused by the defect in the transmission of electrical signals to the brain. LEV is one of antiepileptic drugs which has appropriate pharmacological properties and show its effectiveness in improving the control of the epileptic seizers, its rapid absorbance, and its excretion through the kidneys by glomerular filtration and it is re - absorption into the tubular portion.Kidney arise from intermediate mesoderm and it consists of three execratory organs in all vertebrates : pronephros which is transient and present in all vertebrates, mesonephros which also present in all vertebrates embryos and their activity depends upon the type of embryo, and metanephros which is the functional kidney during life after the embryogenesis in reptiles, birds and mammals. The first signals for kidney initiation in albino rat embryo take place in the 10th days of pregnancy. The current study is designed to demonstrate the effect of LEV (350 mg/kg/day) on the kidney development of Albino rat fetus from the age of 8 - 18 days of pregnancy and its effect on the kidneys of the 3 and 7 days aged newborns. In addition to its effect on pregnant rat kidneys treated with the drug for 17 days. Hundred pregnant Albino rat (Rattus rattus) are enrolled in this study, classified blindly to 50 control and 50 pregnant rats are orally treated with LEV (350 mg/kg/day). The histological results of the current study revealed that there is no effect on embryonic kidney in fetuses aged 9 days of pregnancy, whereas, the pronephros of the fetuses aged 10 days shows death of the cells in comparison to control group. Moreover, the kidney of the 11th aged fetuses showed degeneration of the mesonephric duct represented by decrease cellular mass, death and hypertrophy of some cells comparing to the cells of control group. Concerning the kidney of the 12th aged fetuses and subsequent days, there is degeneration of the epithelial cells of the metanephros duct, their detachment from the basement membrane and their congestion in comparison to the cells of the control group. The kidney of the 13th aged fetuses showed swelling, damage for some of the epithelial cells of the glomerulus and collecting tubules, accumulation and shrinkage of glomerular cells, damage, death, and detachment of some collecting tubules cells demonstrated in the 14th aged fetuses. There enlargement of capsular space in addition to the most previous defects was seen in the 15th aged fetuses in comparison to the control group. Most of the previous defects are seen in the 16th, 17th and 18th age fetuses. Scanning electron microscope revealed nuclear enlargement of the podocytes found in the visceral layer of the renal corpuscle of the 16th aged fetuses (mean diameter 64.6 µm compare to 39.2 µm of the control group). Regarding the kidney of 3rd and 7th aged newborns, the histological defects seen in the latest aged fetuses was also seen and there was statistically significant differences (p?0.05) between the diameters mean of the glomerulus of the 3rd and 7th aged newborn. Whereas, there was no significant difference in the diameter of the kidney tubules of 3rd but there was significant difference of the 7th aged newborn. Regarding the kidney of the 17th day treated pregnant rat, there was enlargement of the capsular space caused by accumulation, and degeneration of glomerular cells characterized by loss of the nucleus, shrinkage of the glomeruli, and atrophy of most of them. Loss of the nucleus and detachment of the cells, and swelling of most of the collecting tubules. There was a statistically significant difference between the diameter of the kidney tubules of the pregnant rat and the control group. In conclusion, LEV had histological effect on the kidney of the 10 - 18 aged fetuses and on 3, 7 days aged newborns in addition to its effect on the kidney of the pregnant female treated with the drug for 17 days.

التحري عن بعـض عوامل الفوعة لبكتريا المكورات Enterococci المعوية المقاومة للمضاد الحيوي الفانكومايسين == Detection of Some Virulence Factors of Vancomycin - Resistant Enterococci

Author name: حيدر صباح كاظم الخماسي
Supervisor name: مي طالب فليح
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: تم الحصول على (20) عزله لبكتريا المكورات المعوية البرازية من اصل (135) عينة جمعت من مستشفى مدينة الطب ومستشفى الكندي وهي كما ياتي (15 عينة الادرار, 60 عينة دم, 50 عينة من قنوات الجذر للاسنان و10 عينة من الحروق).اختبرت الحساسية لهذه العزلات العشرون لـ 11 | Clinical sampling was carried out between September and December 2013, Twenty Enterococcus faecalis isolates were obtained from 135 clinical specimens. The samples included of patients in Medical City Hospital and Al - kindy Hospital (15 urine, 60 blood, 50 root canal and 10 wound swabs) Antibiotics susceptibility test for 20 isolates was done against 11 antibiotics, it was revealed that the isolates showed multi drug resistance were (18) isolates. The vancomycin susceptibility was determined by the minimum inhibitory concentration (MIC). Resistant and intermediate resistant to vancomycin was distributed among isolates at a ratio of 65% and 20%, respectively. Imipenem was found to be the most bactericidal agent against E. faecalis isolates E. faecalis virulence factors were detected phenotypically, The results showed that all isolates (100%) were hemolysin, protease and aggregation substance producer. 30% of isolates showed an ability to produce gelatinase. While (40.7%) of the isolates were a lipase producer. The results of the tube method showed that all E. faecalis isolates (100%) were slime layer and biofilm producer but the amount of adherent layer were different among the isolates ranged from strong to moderate and weak.The extracted DNA was subjected to Polymerase chain reaction (PCR) technique in a monoplex pattern to amplify the virulence factor Enterococcal Surface Protein (esp) which is chromosomal, results of this investigation showed that 20 (100%) E. faecalis isolates gave the amplicon size 933 base pair for the esp gene.The genetic determinants of Vancomycin - Resistant vanA and vanB genes were amplified using monoplex and multiplex PCR techniques in order to identify vancomycin resistant (van+) and sensitive (lacking van) among (13) E. faecalis. The vanA, vanB genes were detected in 11 and 4 E. faecalis isolates, respectively. The results of monoplex and multiplex PCR revealed that the molecular weight of vanA and vanB genes were 550 and nearly 600 bp, respectively. The results revealed that the vanA and vanB amplicons have a genetic variation in their molecular weight during the electrophoresis of PCR product.

دراسة العوامل الوراثية والخلوية والتوصيف الجزيئي لاصناف الحنطة المزروعة في محافظة النجف باستخدام مؤشرات التتابعات البسيطة المتكررة SSR == Studying The Genetic And Cellular Factors And Molecular Characterization of Wheat Varieties Cultivars In The Province of Najaf By Using Simple Sequences Repeat Ssr Markers

Author name: صبا عبد المطلب المدني
Supervisor name: ميثم عبد الهادي السوداني | سلوى جابر العوادي
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: ضمن البحث دراسة عشرة اصناف من الحنطة الناعمة (حنطة الخبز) Triticum aestivum L. من الاصناف العراقية والمدخلة الى البلد من قبل وزارة الزراعة العراقية والمزروعة في محافظة النجف وهي : الشام6 , ابوغريب, تموز2, تموز3, العراق, النور, اللطيفية, اباء 99, الرشيد, و| The study included studying ten cultivars of soft wheat (wheat bread) of several types the Iraqi, foreign and imported types into the country by the Iraqi Ministry of Agriculture and cultivated in the province of Najaf, they are : Sham 4, Abu Ghraib, Tamoz 2, Tamoz 3, Iraq, Alnoor, Latifiya, Ebaa 99, Rasheed and Alforat to study the genetic stability using cytological studies. Cultivars were grown in Petri dishes and pots for the cytological studies that included studying the number of chromosomes in the somatic cells at the root tips and the chromosomal behavior during meiosis of the mega spores of the Pollen Mother Cells (PMCs). The cytological studies regarding the number of somatic chromosomes in the meristematic cells of the root tip revealed that they were in genetic stability regarding the level of chromosomal number and in a state of Eupliod in all the investigated cultivars. They also showed that the chromosomal behaviors during meiosis of the pollen mother cell (PMCs) was bivalent representing organized chromosomal behavior in the investigated cells which is regarded as an indicator and a proof of genetic stability of the studied cultivars. During the study, the genetic cultivars, using 15 primers of simple sequence repeat (SSR), gave all polymorphic between the cultivars and produced polymorphic 39 alleles at average of 2.6 for each locus, showed the genetic adendrogram of ten cultivars was performed by two groups in which the percentage of similarity between them was 75% as the results showed that the closest cultivars to each other were Tamoz 2 and Ebaa 99 which is the same genetic dimension between Tamoz 3 and Ebaa 99 reached 0.107 and by the similarity 89%, while the two cultivars alsham4 and Al - Rasheed were divergent (0.333) with 67% similarity.

تشخيص المسببات البكتيرية والفايروسية لخمج الاغشية السحائية في مدينة الموصل == Detection of Bacterial And Viral Causes of Meningitis In Mosul

Author name: قاسم مصطفى خالد
Supervisor name: علي صالح حسين الجبوري | اميرة محمود محمد الراوي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة اجراء عدد من الاختبارات العيانية والكيميائية على عينات سائل النخاع الشوكي (CSF) cerebrospinal fluid البالغة 152 عينة والتي جمعت من الاطفال الراقدين في مستشفى الخنساء التعليمي ومستشفى ابن الاثير التعليمي في مدينة الموصل لحديثي الولادة لغاية 1 | This study includes performing of macroscopical and chemical and Microbial tests on 152 samples of cerebrospinal fluid (CSF) collected from hospitalized children in Al - khansaa and Ibn - Al - Atheer teaching hospitals in Mosul city with ages ranged from newbornn to 12 years old. Suspected to be infected with meningitis during the interval from August 2009 till April 2010.The results showed that 46.1% of samples were normal with respect to colour and appearance as well as variations in the levels of the three variables including glucose, protein and total cell count with differential cells in the studied samples.The study also included isolation and identification of gram positive and negative bacteria, morphological, cultural and biochemical tests and API were done depending on identification of the isolated bacteria.Many types of isolated gram positive bacteria belonged to the two genera staphylococcus and streptococcus; the results revealed that S.aureus was the more predominant within gram positive causes. Well as many types of gram negative bacteria were isolated represented with Klebsiella pneumoniae, Haemophilus influenzae, Acinetobacter spp. E.coli and Neisseria meningitidis with different percentages with dominance of K.pneumonia and H.influenzae.The study also included screening of some viral causes of meningitis from (35) CSF samples which gave negative culture results by using qualitative Enzyme immunoassays technique using Minividas apparatus.The results showed positive results with 14.2% for two types of virus including Herpes simplex virus (HSV) and Cytomegalo virus (CMV).The study indicated that males were more compared with Females with 55.9% and 44.1% to males and females respectively and highest infection rate occurred in 1 day - 2 years old.Statistical analysis showed the presence of significant correlation between sex, age and the three varieties glucose, protein and total cell count the results showed there is no significant differences between the studied factors.The study also includes sensitivity for some antibiotics, the results showed that gram positive bacteria were the more sensitive to Vancomycin and Cefatriaxone as well as Clindamycin while gram negative bacteria showed sensitivity and resistance percentages of bacteria in general were varied to other antibiotics under study.

دراسة تاثيرات سم الزيرالينون في حبوب الذرة الصفراء والحنطة على بعض المعايير الحيوية لدى اناث الجرذ الابيض وامكانية المعالجة الحيوية للحد من تلك التاثيرات == Study The Effects of Zearalenone Toxin In Maize And Wheat Grain On Some Vital Parameter Among Female White Rat And The Possibility of Biological Treatment To Reduce These Effects

Author name: محمد فخري حسين الخفاجي
Supervisor name: سامي عبد الرضا علي الجميلي
General topic: Biology
Specific topic: Plant - Toxins
Degree: Master
Language: Arabic
University location: Karbala
First pages:
Abstract: هدفت هذه الدراسة الى التحري عن سم الزيرالينون في حبوب الذرة الصفراء (Zea mays) والحنطة(Triticum aestivum) في محافظتي كربلاء وبابل واختبار فاعلية المستحضر الحيوي لبكتريا subtilis Bacillus في حماية حبوب الذرة الصفراء والحنطة من الاصابة بالفطر Fusarium grami | This study aimed to investigate Zearalenone toxin in yellow corn (Zea mays), wheat (Triticum aestivum) in the provinces of Kerbala, Babil and test the effectiveness of the vital preparation for bacteria Bacillus subtilis in the protection of maize and wheat grain from getting the fungus Fusarium graminearum under normal storage conditions. Moreover been conducting a study on the possibility of employing the vaccine bacteria B. subtilis after her killing in thermally protect vital systems for female white rat poison from the effects Zearalenone toxic.Isolation and diagnosis results showed the presence of many different races and fungal species and was the most presence in maize and wheat grain genus Aspergillus spp. and Fusarium spp. Emergence rates reached 58% and 33.3% respectively in maize grain and by 66.6% and 25% respectively in the grain of wheat. The test demonstrated the ability isolates fungus Fusarium spp. results To produce Zearalenone toxin using technology (TLC) Thin Layer Chromatography having 10 isolates that produce the poison out of 12 isolation or by 83.3%. On the other hand diagnosed fungal species F. graminearum and F. moniliform and F. culmorum as productive poison Zearalenone while isolation F.napiform were not able to produce the toxin. The results of the investigation of contamination of samples of yellow corn and wheat Zearalenone using technology TLC presence of contamination and the two rates stood at 41.6% in maize and 25% in wheat grain cereals The results of the study of the effect of pH and sodium chloride in the growth of fungus F. graminearum that this fungus can grow a wide range of pH and ranges from 12 - 6 as well as afford salt levels ranging between 1 - 4 PPT Per 100 ml. This study demonstrated the effectiveness of the vital preparation for the bacteria B. subtilis in the protection of maize and wheat grain from injury F. graminearum under normal storage conditions for a period of three months, not the effectiveness of these bacteria to protect maize and wheat grain in the normal storage conditions are limited but had a distinct role in the protection of critical systems to female white rat when treated those animals vaccinated with bacteria murdered thermally followed congection Zearalenone toxin as any symptoms in some biochemical and physiological blood parameter studied as well as the safety of the investigated organs such as the liver, kidney, Small intestine, ovary and uterus of any medical condition did not appear at a time appeared in which variations in satisfying those criteria have studied female albino rat treatment Zearalenone toxin only.

التحري عن الطفرات في جيني CNTNAP2 وIL1RAPL1 في مرضى التوحد == Mutation Screening of CNTNAP2 And IL1RAPL1 Genes In Autistic Patients

Author name: بشير كاظم خرميط
Supervisor name: عبد الكريم عبد الرزاق القزاز
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: ان اضطرابات طيف التوحد هي مجموعة من الظروف التي تتميز بضعف في التواصل الاجتماعي ونمطية في السلوك. يختلف الاشخاص المتوحدين اختلافا كبيرا في التطور المعرفي والتي يمكن ان تتراوح من فوق المتوسط الى العجز في التفكير. رغم ان اضطرابات طيف التوحد هي تورث بشكل كبي | The autism spectrum disorders (ASDs) are a group of conditions characterized by impairments in reciprocal social interaction and communication, and the presence of restricted and repetitive behaviors. Individuals with an ASD vary greatly in cognitive development, which can range from above average to intellectual disability (ID). While ASDs are known to be highly heritable (~90%), the underlying genetic determinants are still largely unknown. The research studies correlate between Contactin - associated Protein - Like 2 (CNTNAP2), Interleukin - 1 Receptor Accessory Protein - Like1 (IL1RAPL1) genes and ASDs. This study includes forty autistic patients and forty non autistic children as control groups (twenty unaffected sibling and twenty unrelated children). The age of autistic and non autistic children was ranged from 3 to 10 years. Blood samples of autistic patients were collected from Rahman specialist centre for the care and service autistic children in Baghdad. DNA was extracted from blood samples for molecular detection of CNTNAP2 and IL1RAPL1 mutations associated with ASDs by the use Polymerase Chain Reaction (PCR) technique and sequence analysis. PCR reaction was performed to amplify exons (14, 17 and 20) of CNTNAP2 gene that encode to CASPR2, a member of the neurexin family which functions in the nervous system as cell adhesion molecules and receptors. The PCR results revealed that identical bands related to the CNTNAP2 exons were present in all samples. Therefore, five samples (four from autistic patients and one from control sibling) were selected for genotype analysis of CNTNAP2 exons (14, 17 and 20) by direct sequencing. Genotype analysis revealed that there were no any variants in CNTNAP2 exons, but it shows that four different mutations were identified in non coding region (introns) of the CNTNAP2 gene. These mutations were seen only in autistic patients but absent in control sample. Three of these mutations are single nucleotide polymorphisms (SNPs) (rs3779031 A/G in 2118282 position, rs3779032 A/C in 2118436 position and T/G in 2117905 position). The other mutations were deletion in one nucleotide (Del A/ - in 2117901 position). SNP rs3779032 A/C are located at intron 21 while other mutations are located at intron 19. The current study showed that two common SNPs (rs3779031 and rs3779032) in CNTNAP2 were strongly associated with ASDs, where the frequencies of these SNPs were relatively high. SNP rs3779031 identified in two autistic patients while rs3779032 identified in three autistic patients from four unrelated families with ASDs. PCR reaction also was performed to amplify exons (3, 4, 5, 6, 7, 8 and 9) of IL1RAPL1, a gene implicated in calcium - regulated vesicle release and dendrite differentiation. The PCR results show a large intrgenic deletion (Deletion of exons 3 and 4) in six autistic patients, two of these patients were twin. This deletion may be incomplete penetrance due to phenotypic heterogeneity of these patients. This study provides evidence of the role of genetic factors in the etiology of ASD and the important CNTNAP2 and IL1RAPL1 genes mutation of pathogencity ASDs.

استجابة نبات السلق (Beta vulgaris var. cilca L.) للشد الملحي وحامضي البرولين والجبرلين == Response of Chard Plant (Beta Vulgaris Var. Cilca L.) To Salt Stress, Proline And Gibberellic Acids

Author name: حيدر عبد الامير مظهور
Supervisor name: انتصار حسين مهدي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: نفذت التجربة في الموسم الشتوي للعام (2012 - 2013) م في احد الحقول التابعة لناحية الحر في محافظة كربلاء, لمعرفة استجابة نبات السلق للشد الملحي والرش بحامضي البرولين والجبرلين والتداخل بينهما في بعض الصفات الكمية والنوعية لبعض الاجزاء الخضرية والتكاثرية (ا | An experiment was conducted in the winter season of the year (2012 - 2013) in a private Al - Hur district in Karbala governorate, to find out chard plant response to salt stress, proline and gibberellic acids and their interaction on some quantity and quality characteristics to some parts of vegetative and reproductive (leaves, flowers and seeds) to chard plant (Beta vulgaris var. cilca L.).The design of the experiment was randomized complete blocks (RCBD) in a factorial arrangement with three replicate. The first factor was four levels of saline water (0(River water), 2, 4 and 6) ds. m - 1 and the second factor was seven concentration of proline acid (PA) and gibberellic acid (GA3) (0(River water), 50PA, 100PA, 150PA, 50GA3, 100GA3 and 150GA3) mg. L - 1. Means were compared by using averages revised least significant difference (RLSD) at 0.05 probability level when the treatments referred to significant effect. The results showed : 1 - The negative effect of salinity levels on the qualities of the vegetative plant chard before flowering stage with the except of the dry weight of the root system, which had increased significantly with the level of salinity 2 ds. m - 1.2 - The negative impact of salinity levels in vegetative qualities of chard plant after flowering except root length, leaves chlorophyll content (a, b and total), nitrogen%, protein% and proline which increased with the level of salinity 2 ds. m - 1. as well as the potassium% in leaves with a salinity level 4 ds. m - 1 and dry weight of shoots with all levels of salinity and especially 6 ds. m - 1.3 - Gibberellic acid was dominant on proline acid in terms of chard plant by 150 mg. L - 1 concentration before flowering and 50 mg. L - 1. concentration after flowering while proline acid was dominant on gibberellic acid in dry weight before flowering and root dry weight of shoots after flowering, by 50 mg. L - 1 and potassium% in leaves and the protein% with 100 mg. L - 1 concentration.4 - The interaction between the salinity levels and acids concentrations add showed a significant difference in most of the traits of the plant, especially in combinations consisting of level 2 ds. m - 1 or comparison of saline with gibberellic acid by 150 mg. L - 1 before flowering and 50 or 100 mg. L - 1 after flowering while gave a combination of salinity level 4 ds. m - 1 with concentration of proline acid 100 mg. L - 1 higher of potassium% in leaves

دراسة بيئة وتنوع الهائمات الحيوانية في نهر الغراف واسط - العراق == Ecology And Diversity Study of Zooplankton In Al - Gharraf River Wasit - Iraq

Author name: رشا موحان سلمان
Supervisor name: جميل سعد السراي | مهند رمزي نشات
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Wasit
First pages:
Abstract: جريت دراسة بيئية للهائمات الحيوانية في نهر الغراف, وموقعه في الجزء الجنوبي الشرقي من العراق, تحيط به مساحات شاسعة وخصبة من الاراضي الزراعية. هدفت الدراسة الى التنوع الاحيائي للهائمات الحيوانية, من خلال قيم بعض ادلة التنوع الاحيائي, وكذلك دراسة تاثيربعض ال | An ecological study of zooplankton was carried out on the Al - Gharraf River, that located in the south eastern sector of Iraq and surrounded by vast and agricultural land to the aim of this study are biodiversity of zooplankton by through some of biodiversity index values of some physical and chemical characteristics of water on the biodiversity and abundance of Al - Gharraf zooplankton. The samples of the study have been collected monthly for the period from August 2014 untill July 2015 at three selected stations in the river; Station one is located at 500 m of the AL - KUT Dam. The second is situated at a distance of 15 Km away from the station one at (Kut - Muwafaqiya) and the latter station is located at 18 Km apart from the second one upon entering the river to Muwafaqiya The study was achieved by measurement of some physical and chemical properties of water were analyzed the parameters studied included Air temperature (11 to 38.6) ?C, water temperature (12.1 to 29.1) ?C, pH (7.2 to 7.9), EC (490 to 1860) µS/cm, Salinity (0.31 to 1.19) ‰, DO (5 to 9.1) mg/L, BOD5 (0.2 to 7.1) mg/L, Turbidity (4.69 to 74) NTU, TDS (0.26 to 0.99) g/L, TSS (0.005 to 5.9) mg/L, TH (388 to 520) mg/L, Ca+2 (100.2 to 180.36) mg/L, Mg+2 (3.69 to 52.07) mg/L and NO3 - 2 (0.034 to 3.58) mg/L and PO4 - 2 (0.011 to 1. 25) mg/L., Bicarbonate (130 to 185) mg/L, Sulfate (80 to 180) mg/L, the percentage of oxygen saturation (48.59 to 93.82) %, Water flow (14.28 to 76.92)cm\sec. and total Alkalinity(119 to 274)mg/L.The study also included biological aspects using density, Rrelative abundance index (Ra), Constancy index (S), Species richness index (D), Shanon - Weiner index (H), Species uniformity index (E) and Jaccard presence - community were also employed to determine the values of species composition, density and zooplankton biodiversity in the study area. In the current study about 113 Taxonomic units of zooplankton were identified, the rotifer was dominate group including 64 taxa to rotifera, 34 taxa belonging to cladocera and 15 taxa to copepod.The Zooplankton showed high density in river 8332.62 Ind. /m3 during April 2015 while lower465.32Ind. /m3 in Sebtember 2014 also the rotifer recorder density from198.66 to 7865.98 Ind/m3, cladocera density from 0 to 233.32 Ind/m3and copepod density from 66.33 to 2932.65 Ind/m3.The results of the relative abundance index showed that the species rotifera : neplunia Rotaria. Keratella valga Brachionus angularis, Keratella cochlearis dolicopter While as from Cladocera, Alona rectangular, Polyarthra Alonella excise, , Moina affinis, , Bosmina longirostris Ceriodaphnia rigaudi Chydorus piger, Scapholebrus kigni. Simocephalus sevrulatu and the copepods : Immature Harpacticoida, Paracyclo fimbriatus, Nitocra sp., Nitocra spinipe, , Harpaticoida sp. and Nauplii were more abundant in Al - Gharraf river. also the results of Constancy index showed Six taxa belonged to zooplankton, which were considered "Constant" at stations However the other taxonomy units varied from "accessory" and "accidental" taxonomy units in study stations. The values of the Species richness index of rotifer varied from 0 to 5.42 and cladocera varied from 0 to1.41 and copepod from 0 to 2.70 The highest percentage of similarity 48.148% for Zooplankton were reported between stations 1 and 2. The lowest similarity reaching 35.412% recorded between stations 2 and 3. The Shanon - Weiner index of total Zooplankton varied from0.4to 2.5 bit/ind while the Shanon index of Rotifera from1.05 to 2.61 bit/Ind., Cladocera varied from 0.4to 1.38 bit/Ind. and Copeoda varied from 0.11 to 1.76 bit/Ind., The uniformity index of total Zooplankton varied from0.17 to 1.01and Rotifer varied from 0.4 to 0.9, Cladocera from 0.4 to 1 and Copeoda from 0.08 to 0.99 These high values indicate that there is no ecological stress on zooplankton in the study area

دراسة تاثير الملوثات المنبعثة من المركبات على الاشخاص في شوارع مدينة تكريت == Study The Effect of Pollutants Emitted From Vehicales Upon Blood of Workers In Tikrit City Streets

Author name: رغد مقداد محمود الحمداني
Supervisor name: جهاد ذياب محل الجنابي | صالح محمد رحيم العبيدي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: استهدفت الدراسة الحالية التي جرت في شوارع مدينة تكريت قياس تراكيز بعض الغازات المهمة التي تطرحها المركبات الى البيئة وهي احادي اوكسيد الكاربون, ثنائي اوكسيد الكاربون, ثنائي اوكسيد الكبريت (CO, CO2, SO2) وكذلك دراسة نوعية مياه الامطار المتساقطة على منطقة ا | The current study which takes place in the main streets of the city of Tikrit, aims at measuring the concentrations of some essential gases which are emitted by the vehicles of the environment. These gases include Monoxide Carbon, Dioxide Carbon and Sulfur Dioxide (CO, CO2, SO2). This study also aims at investigating the quality of rainwaters which are falling on the area of the study, and conducting some biological tests for them. Moreover, this study aims to identify the effect of vehicles productions upon blood physiology and the construction of some persons who are dealing with them. The samples have been collected during September 2010 till May 2011, and then classified in terms of age and duration of exposure. Alsuqoor Village represents the control group. A sample has been collected from healthy, non - smoker males. The concentrations of some polluted gases emitted from exhausted vehicles are identified. They are significantly high in comparison with the control group and during the whole chapters of the study. The highest level of pollution value is recorded by the gases (CO, CO2, SO2) and at (p< 0.05) level in Albasha Street during the Autumn, whereas the lowest value is recorded in Alzuhoor Street, during the Winter ( ). In addition, some of rainwater characteristics have been measured such as, (Ph, electrical m and the amount of plankton in water). The obtained results show that there is a significant decrease in PH concentration and a significant increase in EC concentration. Moreover, results indicate that the highest concentration of the clot material is in Alarbaeen Street ( ) whereas, the lowest concentration is in Alzuhoor Street ( ). The level of some rare elements has been measured in the blood serum of those who are exposed to (lead, cadmium) and in different periods of times. A significant increase is recorded in the levels of those elements, in comparison with the control group (ps 0.05). As for effect of blood components of those who are exposed to pollutants, the concentrations of both (Hb, Pcv) have been decreased with an increase in the concentrations of (the number of red and white cells and ESR) in comparison with the control group at level (P<0.05). It is also noticed that there is a significant increase in the number of the neutral and acidic white blood cells with a significant decrease in the number of the moral white blood cells at the lymph cell, in comparison with the control group. Results also show that there is a significant increase (P<0.05) in the level of each (Cholesterol, Triglycerides, and Lipoproteins with low density LDL) with decreased concentrations of (High Density Lipoproteins HDL) whereas, there are no significant difference in the concentrations of (Low density Lipoproteins Very high density VLDL). Concerning the concentrations of (Urea, Uric acid, Kreatenin) there is a significant increase (P<0.05) in comparison with the control group and for all the groups of the study. The pollutants have an effect upon the average number of enzyme in the blood serum. There is a significant increase in the average of the enzymes that carry amino (ALT, AST) an enzyme phosphate in comparison with the control group. The study has also shown a significant increase in the Monoaldehyde level MDA and decrease in the glutathione level, under the effect of various pollutants. It is noticed that the blood components as well as the biochemical variable are greatly affected as the period of exposure extended and these biochemical variables become very great. Therefore, most of the differences are existed in the third group whose ages range between (40 and 49) with 9 years of work, followed by the second group whose ages range between (30 and 39) with 7 years of work. Then comes the first group whose ages range between (20 and 29) with 5 years of work, in comparison with the control group

دراسة وبائية لبعض مسببات الاسهال من الاوالي الطفيلية في المرضى المراجعين لمستشفى صلاح الدين العام في تكريت مع دراسة تجريبية لتاثير المستخلص الكحولي لنبات Lantana camara عليها في الفئران المختبرية == Epidemiological Study For Some Diarrhea Causes From Parasitism Protozoa For The Patients That Attended General Salahalddin Hospital In Tikrit With Experimental Study For The Effect of Alcoholic Extract From Lantana Camara Plant On It In Experimental Mice

Author name: رواء محمود هاشم
Supervisor name: توفيق ابراهيم محمد الالوسي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة للمدة من 2010 /11/1 الى 2011/11/1.وتضمنت فحص 1002 عينة من غائط مصابين بالاسهال تراوحت اعمارهم ما بعد الولادة الى اثنتي عشرة سنة من العمر راجعوا مستشفى صلاح الدين العام في تكريت. اشتملت الدراسة على جزءين تمثل الجزء الاول بدراسة وبائية، ت | This study made on the period between (1/11/2010) to (1/11/2011) that included testing (1002) diarrhea patients excrement samples, their ages were between after birth to twelve years that had attended General Salahalddin Hospital.The study included two parts. First epidemiology study, collecting samples and making the laboratory tests that accomplish with 2 methods, the direct method and deposition method to detect the percentage of infected by some parasitism protozoa that cause the diarrhea according to a lot of standards that included, age, sex, class, nature of suckle, number of family members, the source of water, mother education, sequence of the child in his family and the season of infection, the laboratory test assured that from the total (582) , (58.08 %) are infected with parasitism diarrhea compare with other diarrhea cases that was (420) , (41.91%) and reached to the following : - The infection with E.histolytica diarrhea was (492), (%84.5) and infected with G.lamblia diarrhea was (90) , (15.46%). Number of infected males with E.histolytica and G.lamblia parasitism as follows(8.24%)(46.39%) and infected females with E.histolytica and G.lamblia parasitism as follows (7.21%) (46.39%).The highest infection with E.histolytica and G.lamblia parasitism was the ages between (4 - 6)years and the percentage was as follows (4.81%, 29.89%). The highest infection with amebic tissue and G.lamblia parasitism that came from country were (9.10%, 60.82%) also noticed that the raise of infection with E.histolytica and G.lamblia parasitism among children that their mothers had low education less than intermediate (illiteracy and intermediate) as follows (29.8%, 36.5%) for the children were infected with G.lamblia parasitism. And for children that infected with E.histolytica parasitism among children that their mothers had low education less than intermediate (illiteracy and intermediate) as follows (4.29%, 36.5%). The percentage for infants for the first 2 years had been infected with E.histolytica and G.lamblia parasitism for the first 2 years that they depend on industrial suckle as follows (7.73 %), (41.2%). The percentage for infants above 2 years had been infected with E.histolytica and G.lamblia parasitism for the first 2 years that they depend on industrial suckle as follows (9.10%) , (48.96%) also noticed the raise of infection with E.histolytica and G.lamblia parasitism among the children that was the river is the water source, the percentage were as follows (8.59 %), (51.54%). And the infection with E.histolytica and G.lamblia parasitism among the children that was the pipes system is water source the percentage were as follows (6.87%), (23.98%). the infection were very high with E.histolytica and G.lamblia parasitism among the children that lives within crowded families, the percentage was (12.54%), (55.67%). and the infection with E.histolytica and G.lamblia parasitism according to the seasons was dissimilar, and the highest recorded infection with E.histolytica parasitism in winter as follows (35%) and the lowest infection was (11.3%) in summer. Meanwhile the highest infection with G.lamblia parasitism (8.41%) in summer and (1.20%) in autumn.The second part studied the effect of alcoholic extraction from lantana camara plant on growth of E.histolytica and G.lamblia parasitism on mice. The effect was obvious for the alcoholic extraction for the mentioned plant for all extraction on the E.histolytica and G.lamblia parasitism in general, but the most effectiveness extraction to destroy the E.histolytica and G.lamblia parasitism was 1000 mlm/kgm from the alcoholic extraction for the plant lantana camara
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