المستودع الرقمي العراقي

Iraqi Digital Repository

تقدير البعد الوراثي لبعض اصناف نخيل التمر Phoenix dactylifera L. المزروعة في العراق باستعمال تقانة RAPD == Estimation of the Genetic Distance for Some Date Palm Varieties ( Phoenix dactylifera L.) Grown in Iraq using a RAPD Technique

اسم المؤلف: مهند رشيد حسين الزيدي

اسم المشرف: شذى عايد يوسف

الموضوع العام: علوم الحياة

السنة: 2014

الموضوع الدقيق: النبات

الدرجة: ماجستير

الجامعة: جامعة ديالى - كلية التربية للعلوم الصرفة - قسم علوم الحياة

اللغة: العربية

مكان الجامعة: ديالى

الصفحات الاولى: 📎 24T3119 - p.pdf

المستخلص: This study was conducted at Molecular Biology lab, Genetic Engineering Department, Agricultural Research Directorate, Ministry of Science & Technology to estimate fingerprint and genetic relationship among 9 Iraqi varieties of date palm Phoenix dactylifera L. namely Asabee AL - aroose, Zahdi, Sukari, Saada, Ashrasy, Barben, Shetwi, Khistawi and Nhair Al Saly. 20 primers were used to PCR amplify characteristic Random Amplified Polymorphic DNA (RAPD) marker fragments. Total genomic DNA was isolated from varieties fresh leaves, the average yields of DNA were in the range of 100 - 522.5 ng/ul with purity ranged between 1.66 to 1.92. Polymerase Chain Reaction (PCR) experiments were performed to detect DNA polymorphisms amplified fragments in each cultivar (their numbers and molecular weight sizes) on agarose gel electrophoresis stained by ethidium bromide. Results of RAPD experiments revealed differences in band numbers and molecular weight sizes, all tested primers yielded 157 polymorphic bands and the lowest polymorphic band numbers (5 bands) were with primers OPA - 01 and OPA - 20 while the largest number of polymorphic (12 bands) were with primers OPA - 08 and OPH - 09. RAPD analysis showed that many primers gave unique band in all samples made possible fingerprint identification of each cultivar, Ashrasy variety gave highest 6 unique polymorphic bands with primers OPA - 08, OPC - 04, OPF - 05 and OPF - 12, while Zahdy variety gave 1 unique polymorphic band (385 pb) with primer OPB - 05. Data was analyzed by using statistical program SPSS to find similarity and genetic relationship among cultivars, similarity index ranged between 0.291 and 0.511 and the biggest percentage of similarity was between Saada and Shetwi. Dendogram analysis has separated varieties into two main groups, the first main group consists of three sub - cluster or sub - group, the first sub - cluster contained Saada and Shetwi varieties while the second sub - cluster included Zahdi, Ashrasy and Nhair Al Saly and the third sub - cluster consisted Asabee Aroose cultivar. The second main group was divided into two sub - group, the first sub - group consisted of Berben and Khistawi while the second sub - group included Sukary.