دراسة بكتريولوجية لبعض مسببات الاسهال لدى الاطفال == Bacteriological study of the some causes of diarrhea in children
Author name:
ريا خليل ابراهيم القيسي
Supervisor name:
عباس عبود فرحان الدليمي | هادي رحمن رشيد الطائي
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Diyala - College Of Education For Pure Sciences - Department Of Biology
Language:
Arabic
University location:
Diyala
First pages:
24T3123 - p.pdf
Abstract:
This study included The investigation of the existence some type of Enterobacteriaceae bacterial Causes Diarrhea in (215) samples for patients sufferers symptoms indicate that their infection Diarrhea. period from 1\10\2014 to 30\1\2015, and used MacConkey agar and blood agar for the cultivation of these samples.The results refer that 65 isolates are belonging to bacteria of Enterobacteriaceae 13 (20 %) Proteus mirabilis and 3(4.6%)Morganella morganii 2(3.1%) Salmonella spp by using diagnostic phenotypic ,biochemical tests and confirm the diagnosis by using system Vitek2.The results of the investigation of some virulence factors showed the isolates of Proteus mirabilis produce of haemolysin (92.3%) ,while Salmonella spp and Morganella morganii have not ability to produce this enzyme. The results showed that all isolates of Proteus mirabilis and Salmonella spp and Morganella morganii were produced urease by (100%) (0%) (100%) respectively.The results showed that all isolates of Salmonella spp and Morganella morganii were produced protease by (100%)(50%) while Proteus mirabilis have not ability to produce this enzyme.The results showed that isolates of Proteus mirabilis were produced bacteriocin by (69.2%) while Salmonella spp by (50%) and Morganella morganii have not ability to produce this enzyme The results showed a variance as far as their resistance to these antibiotics. Isolates of Proteus mirabilis showed highest resistance rate (76.9%) for Ampicillin e while isolates of Salmonella spp highest resistance rate (100%) for Cephalothin, Cefotaxime and Ampicillin , while isolates of Morganella morganii showed highest resistance rate %100 for Trimethoprime , Ampicillin , Cephalothin , Nalidixic acid , Piperacilli.The results showed (15) isolates rate (83.33%) have multiple resistance pattern for antibiotic and divided into two groups , first group included (5) isolates which were resistant to ( 2 - 5 ) antibiotics , while second group included (10) isolates were resistant to (6 - 10) antibiotics . Results indicated that the second group was dominant. The results showed that the Resistance prevailing coordinated to isolates Proteus mirabilis is (4) antibiotic (Ap - TM - CTX - NA) and to isolates Morganella morganii is (6)antibiotic (AP - TN - PRL - NA - KF - FOX) and to isolates Salmonella spp is (3) antibiotic (AP - CTX - KF).Results showed content plasmid for Proteus mirabilis and Morganella morganii they do not contain plasmids packages While shown Salmonella spp content on plasmids one package .The results of bacterial conjugation In the liquid medium The success of the process of bacterial conjugation for Salmonella spp That have one package of plasmids before and after of bacterial conjugation .The results of transmission of prescription antibiotics (TM - AP - KF - CTX)
