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تفاعل انزيم البلمرة المتسلسل النسخي العكسي ذي الوقت الواقعي كاداة تشخيصية جزيئية في التحري عن الجينات المدمجة الشائعة في عينة من الاطفال العراقيين المصابين بابيضاض الدم اللمفاوي الحاد == Real time reverse transcriptase polymerase chain reaction as diagnostic molecular tool in screening of common fusion genes in sample of Iraqi children with acute lymphoblastic leukemia

اسم المؤلف: اريج عماد كاظم
اسم المشرف: بان عباس عبد المجيد | سلمى عباس الحداد
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الامراض - الدم
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ابيضاض الدم الليمفاوي الحاد عند الاطفال هو مرض متنوع له اصناف وراثية متعددة تستجيب بصورة مختلفة للعلاج الكيميائي. توجد الكثير من التغايرات الكروموسومية المؤدية الى انتاج جينات مدمجة مصاحبة لحدوث هذا المرض.اهداف الدراسة : لدراسة تعبير الجينات المدمجة الشائعة في ابيضاض الدم اللمفاوي الحاد عند الاطفال باستخدام تقنية تفاعل البلمرة المتسلسل ذي الزمن الواقعي وربط نتائج هذه الدراسة مع مختلف المؤشرات السريرية والمختبرية ثم تقييم هذا التعبير بعد العلاج الاولي.المرضى, المواد وطرق العمل : اجريت هذه الدراسة المستقبلية للحالات والشواهد باستخدام تقنية تفاعل البلمرة المتسلسل ذي الزمن الواقعي لدراسة تعبير الجينات المدمجة MLL - AF4 ,BCR - ABL1 ,E2A - PBX1 ,TEL - AML1 وSIL - TAL1 في ناضح نقي العظم ل 48 مريض بابيضاض الدم اللمفاوي الحاد قبل العلاج مع 46 شخوص ضابطة, تم جمعها من مستشفى حماية الطفل التعليمي / دائرة مدينة الطب للفترة من 1 تموز 2013 الى 31 حزيران 2014. استعمل جين GAPDH كجين مراقب داخلي لدراسة تعبير الجينات المذكورة بعد العلاج.النتائج : من اصل 48 مريض بابيضاض الدم اللمفاوي الحاد, كان هناك 21 ذكرا" و27 انثى مع نسبة ذكور الى الاناث 0.78 : 1 . تراوحت اعمارهم بين 2 شهر الى 13 عام مع متوسط عمر 5 اعوام . تراوحت اعمار 26 مريضا" بين 1 - 5 سنوات. اظهر الفحص الجزيئي الكشف عن نسخ الجينات المدمجة TEL - AML1، E2A - PBX1 وp190 1BCR - ABL في 20.8%, 16.7% و2.1% من المرضى، على التوالي، بينما لم يتم الكشف عن نسخ p210 1BCR - ABL ، MLL - AF4 وSIL - TAL1. بالاضافة الى ذلك، اظهر احد المرضى تعبير نسخي لكل من TEL - AML1 وE2A - PBX1 في الوقت نفسه. وجدت التعابير الجينية بعد بدء العلاج في 2 مرضى من اصل 12 مريض.الاستنتاجات : ان تقنية تفاعل البلمرة المتسلسل ذي الزمن الواقعي طريقة جزيئية دقيقة وقابلة للتطبيق في تصنيف ابيضاض الدم اللمفاوي الحاد في الاطفال. ان نتائج التصنيف الجزيئي للاطفال العراقيين المصابين بابيضاض الدم اللمفاوي الحاد مشابهة نوعا" ما للتقارير العالمية جاعلة TEL - AML1 الجين المدمج الاكثر انتشارا". | Pediatric acute lymphoblastic leukemia is a heterogeneous disease with various genetic subtypes that respond differently to treatment. Many chromosomal aberrations with resultant fusion genes are known to be associated with the disease.Objectives : To study the expression of common fusion genes in pediatric acute lymphoblastic leukemia using a quantitative real time polymerase chain reaction technique, correlate results with different clinical and laboratory findings and evaluate these expressions after initial treatment.Subjects, materials and methods : A case - control prospective study was conducted using qPCR technique to study the expression of TEL - AML1, E2A - PBX1, BCR - ABL1, MLL - AF4 and SIL - TAL1 fusion genes in bone marrow aspirates of 48 untreated acute lymphoblastic leukemia pediatric patients and 46 control subjects, were recruited at the Children Welfare teaching hospital / Medical City directorate for the period from 1st of July 2013 to 31st of June 2014. Post - induction transcripts’ evaluation for 12 ALL patients was done by comparative quantification method using GAPDH as a reference gene.Results : Out of 48 acute lymphoblastic leukemia patients, 21 were males and 27 were females with a male to female ratio of 0.78 : 1. Age ranged from (2 months to 13 years) with 26 patients aged between 1 - 5 years whereas the median age was 5 years. The mean age was 5.9 years + standard error of 0.51 years. Molecular screening demonstrated detection of TEL - AML1, E2A - PBX1 and BCR - ABL1 p190 transcripts in 20.8%, 16.7% and 2.1% of patients, respectively. BCR - ABL1 p210, MLL - AF4 and SIL - TAL1 transcripts were not expressed. One patient expressed both TEL - AML1 and E2A - PBX1 transcripts. Post induction transcripts were detected in 2 out of 12 patients.Conclusions : Real time PCR technique is an applicable molecular method in classifying and predicting prognosis in pediatric ALL. The molecular classification of Iraqi children with ALL is mostly similar to reports worldwide making TEL - AML1 fusion gene the most prevalent type

دراسة التعبير الوراثي للجينات P53, KRAS, c - MYC & Her - 2/neu وانواع الرنا المايكريوية miR - 21, 34a, 92 & 98 على عينات ماخوذة من الغسول القصبي والانصباب الجنبي لمرضى مصابين بسرطان الرئة ومرضى مصابين بامراض رئوية مزمنة غير سرطانية == Expression of P53, KRAS, c - MYC, Her - 2/neu genes and microRNAs 21, 34a, 92 & 98 in cancerous and non - cancerous bronchial wash and pleural aspirate

اسم المؤلف: حسين عبادي الجبوري
اسم المشرف: بان عباس عبد المجيد | عدنان الجبوري
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الامراض
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : Lung cancer is the leading cause of death from cancer in men all over the world. Most of newly diagnosed cases were in advanced stage and beyond radical treatment due to late appearance of worrying symptoms and absence of effective screening method for high risk groups. Nowadays, depending on recent advances in molecular studies researches are directed toward finding molecular markers for diagnostic, therapeutic and prognostic purposes. Aim of study : To investigate the possibility of using expression of P53, KRAS, Her - 2/neu, c - MYC and microRNAs 21, 34a, 92, and 98 as a molecular biomarkers for detection of lung cancer in samples of bronchial wash and pleural fluid. Material and Methods : A prospective case control study on a total of 120 samples, sixty bronchial washes and sixty pleural effusions. The samples were taken from patients recruited at the Thoracic Surgical Unit in the Specialized Surgery Hospital/ Medical City during the period from March 2012 to April 2014. The work was performed in the Department of pathology and forensic Medicine, Baghdad college of Medicine. The specimens were thirty bronchial wash and thirty pleural fluid samples positive for lung cancer cells by cytopathology, and similar number of negative samples. Studied genes were amplified using qRT - Realtime PCR. Housekeeping genes for normalization of mRNAs was GAPDH and RNU - 48 for microRNAs. Expression was calculated using equation; Expression = (2 - ΔΔCt). Results : Results of Ct values for each marker were obtained from Max Pro 5000 Agilent Technology PCR software and raw and standardized Ct values were analysed using SPSS - 22 software. The mean, standard deviation, t - test, ANOVA test and LSD (least significant difference) were obtained before and after normalization. A statistically significant differences in the expressions of p53, KRAS, c - MYC, Her - 2/neu and microRNAs, 21, 34a, 92, & 98 genes were found between positive and negative (control) samples with a significant p - values of <0.05. Conclusion : According to this study a conclusion could be reached; the study of expression profiles of mRNAs of P53, KRAS, c - MYC, Her - 2/neu and microRNAs 21, 34a, 92 and 98 genes can be used as a biomarker in the detection of lung cancer, differentiating subtypes, and screening of high risk groups

استخدام الصبغة المناعية p16INK4a والتهجين الموقعي وتاثيرها على فحوصات عنق الرحم الصحيحة والملتبسة == The Use of p16INK4a and In Situ Hybridization and Their Impact on the Healthy and Equivocal Pap Smears of the Cervix

اسم المؤلف: ثناء جميل الخشالي
اسم المشرف: ندى صالح امين | هدى مهدي الخطيب
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: التشريح والانسجة والاجنة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Throughout the past thirty years, the perception of cervicalcarcinoma has shifted from that of a mysteriously fatal disease, to one ofthe sexually transmitted, human papillomavirus (HPV) related infection.HPV DNA has been found in almost all cervical invasive and preinvasivecervical neoplasms.Conventional Pap smear, which was established in the early sixtiesreduced effectively the morbidity and mortality related to cervical cancer.Given the lower sensitivity of the Pap cytology test, new diagnosticparameters have been established. The histological features ofpreinvasive cervical neoplasia (CIN1, CIN2, and CIN3) are wellunderstood. However, misinterpretation of the morphological criteriacould lead to significant variability.The aim of this study is to identify women with equivocal and normalPap smear who are at risk for developing cervical cancer through the useof p16INK4a immunostaining and human papillomavirus (HPV) DNAtesting using in situ hybridization (ISH).Materials and MethodsIn order to identify human papillomavirus infection in minorcytological and histological abnormalities, biopsies collected from 60women 25 - 66 years of age with normal cervices and Pap smears (groupI), abnormal cervices and Pap smears (group II) and with cervicalcarcinoma (groupIII) referred to the Colposcopy Clinic in BaghdadTeaching Hospital - Medical City Complex, during the period from June2013 through July 2014. Cytology samples collected with ThinPrep forIVliquid base cytology (LBC), punch biopsy for histopathology formalinfixed and paraffin embedded. In situ hybridization (ISH) and p16INK4aimmunohistochemistry in addition to routine hematoxylin and eosin(H&E) stain were used to evaluate the histological specimens.ResultsThe age, age of marriage and parity of the three groups werestudied in relation to each of ISH and p16INK4a reactions. Agedistribution was highly significant among all age groups; in both ISH andp16 test results GI &GII (p=0.00), GI & GIII (p=0.00), GII &GIII(p=0.00) in both ISH and p16 test results. The age of marriage was notsignificant among the groups in both ISH and p16; GI & GII p (0.93), GI&GIII (0.12), GII &GIII (0.30). The effect of parity was insignificantamong all groups; GI & GII = (p = 0.66), GI & GIII (p = 0.82), GII &GIII (p = 0.79) in both ISH and p16 test resultsp16INK4a immunoreaction : Among group I there was a negativereaction in all the specimens collected. In specimens from group II, therewas a very mild or focal p16INK4a immunoreaction in one specimen(5%), a moderate immunoreactivity in 9 specimens (45%), and a highpositive reactivity in 10 specimens (50%) (p=0.000). Group III showedvery high immunoreaction in all the specimens collected.In situ hybridization (ISH) in group I was mild positive in 4specimens (20%) which showed diffuse signal pattern. A mild positivereaction with diffuse nuclear staining was noticed in 4 (20%) of thecervical specimens. Moderate positive reaction with diffuse staining ofthe nucleus was observed in 8 (40%) of the specimens. The remaining 8(40%) of the specimens showed both diffuse and punctate staining of theVnuclei. Among group III specimens, the nuclei in the epithelial cellsshowed both punctate and diffuse signal patterns.Chi square was done to compare results concerning ISH & variousgroups was highly significant (p= 0.00) ConclusionHPV infection is prevalent and can be missed by the conventional Pap smear. The severity of the disease was directly proportional with the age of women in this study. p16 overexpression was correlated with the positivity of ISH. This may support the use of either, or both, tests to improve the accuracy of cervical intraepithelial neoplasia (CIN) diagnosis, and help in the triage of women with equivocal lesions.

دراسة تجريبية لانزيمات البروتييز للزائفة الزنجارية المعزولة من المرضى العراقيين المصابين بتقرح القرنية ودورها في علاج تقرح القرنية المتسبب عن المكورات العنقودية == Experimental Study of Pseudomonas aeruginosa Proteases Isolated from Corneal Ulcer of Iraqi Patients and Their Role in the Treatment of Staphylococcus aureus Keratitis

اسم المؤلف: عائدة حسين ابراهيم
اسم المشرف: جاسم محمد كرحوت | منيرة جلوب اسماعيل
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: One - hundred and twenty samples ( corneal scraping) were collected from patients diagnosed to have microbial keratitis (corneal ulcer) who attended Ibn Al - Haitham Teaching Eye Hospital from the period between May 2013 and November 2013, Pseudomonas aeruginosa was reported 26 (21.6%) from the total cases. All bacterial isolates were diagnosed by conventional and biochemical tests, and confirmed by Vitek 2 Compact System.The role of proteases enzymes ( Elastase ( LasB), LasA, Alkaline protease and Protease IV ) of Pseudomonas aeruginosa in the corneal ulcer was studied by using genetic and molecular biological method by real time PCR, and the results indicated that three bacterial isolates of Pseudomonas aeruginosa possessed elastase gene (LasB) (11.5%), and only one bacterial isolate of Pseudomonas aeruginosa harbored LasA protease gene ( 3.8%). All bacterial isolates of Pseudomonas aeruginosa were harbored alkaline protease gene (100%), and twenty bacterial isolates were harbored protease IV (76.9%).The results of real - time PCR analysis indicated that four bacterial isolates of Pseudomonas aeruginosa (15.3%) were harbored more than one gene of different proteases enzymes ( elastase, alkaline protease, and protease IV).On the other hand our results showed that one bacterial isolates (3.8%) harbored both LasA protease and alkaline protease genes, and twenty bacterial isolates of Pseudomonas aerugenosa (76.9%) were harbored alkaline protease and protease IV genes.The LasA protease was extracted from Pseudomonas aeruginosa isolate by cooling centrifuge and precipitated supernatant by ammonium sulfate at saturation (80%). The resulted extracted crude enzyme concentration was 60 μg/ml. Then the crude enzyme was partially purified by dialysis and gel filtration chromatography by using Sephadex G - 100. The concentration of partial purified enzyme reached 40μg/ml.IIISummaryThe results of the experimental treatment of bacterial keratitis ( in vivo) of infected eyes rabbits caused by Methicillin Sensitive Staphylococcus aureus showed that the efficacy of LasA protease was effective was as Lysostaphin in eradicating Methicillin Sensitive Staphylococcus aureus from the infected corneas after approximately 15 h after giving the drug at dose 100 μl ( concentration 1μg / ml ) . While Vancomycin gave us very little potency in eradicating S. aureusfrom corneas in comparison with potency of LasA protease and Lysostaphin duringthis time but showed good potency very late approximately after 3 days of applicationof treatment.The results of the experimental treatment in vitro (in the test tube ) that is caused by Methicillin Sensitive Staphylococcus aureus showed that the efficacy of LasA protease was similar to that of Lysostaphin drug in the killing of Methicillin Sensitive Staphylococcus aureus in the bacterial broth.

ازدياد تعبيرالمعلمة الجزيئية miR - 21 التي تتوسط هبوط منتظم لجين PTEN في سرطان الثدي ومصاحبتها مع عوامل التنبؤ المعروفة == MicroRNA - 21 overexpression mediated phosphatase with homology to tensin (PTEN) downregulated in breast cancer in association with clinicopathological status

اسم المؤلف: شروق محمد عباس التميمي
اسم المشرف: سالم رشيد حمودي العبيدي | علي حسين الخفاجي
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الامراض النسيجية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الخلفية : - سرطان الثدي هو الاكثر شيوعا في النساء في كل انحاء العالم . الموروث ( PTEN) هو الموروث المثبط للسرطان ,وهو منظم عكسي(اي مثبط)لاهم طريق لحدوث نمو وتكاثر الخلايا .المعلمة الجزيئية مثل ( miR - 21) هي الجزيئة الاولى التي تم اكتشافها التي تساعد على نمو وتكاثر الخلايا النامية وتمنع موت الخلايا ,اي تطيل من عمر الخلايا النامية. اذا حدث تغير في جزيئة ( miR - 21) وازداد عملها عن الحالة الطبيعية تؤدي الى حدوث نمو مستمر بدون موت الخلايا النامية مما يودي الى حدوث الخلايا السرطانية بالاضافة الى تاثيره على الموروث المثبط لنمو السرطان وهو (PTEN )الذي يعتبر موروث مستهدف له, بحيث يستطيع منع عمل هذا الموروث لذلك يؤدي الى حدوث سرطان الثدي .الهدف من الدراسة : - قياس تعبير المعلمة الجزيئية ( miR - 21) والموروث ((PTEN لمعرفة (زياده او نقصان) على اعتبارهم متغيرات حقيقية عن النسيج الطبيعي وعوامل التنبؤ لسرطان الثدي . المواد وطرائق العمل : - هذه الدراسة هي دراسة تجريبية من الشهر الاول سنة 2013 الى الشهر الاول سنه 2015.تم اخذ خمسين زوجا من العينات, وهذه العينات تؤخذ من سرطان الثدي ومن النسيج الطبيعي للثدي من النساء اللواتي تم استئصال ثديهن بسبب السرطان وهذه العينات تم اخذها في صالة العمليات وكانت العينات طازجة. تم استخلاص شريط ال RNA ثم بعد ذلك تم فحص جزيئة ال (miR - 21) وموروث(PTEN) . وتم تطبيق الواسم المناعي النسيجي الكيمياوي ((IHC لهرمون الاستروجين والبروجستين معher - 2 في العينات التي تم وضعها في بلوكات الشمع وبعد ذلك تم اختيار الحالات الموجبة ل(her - 2)وفحص تضاعف الموروث ( HER - 2) بواسطه تهجين موضعي ذي لونين .النتائج : - معظم الحالات كانت جزيئة ال( miR - 21) مرتفعة في النسيج السرطاني عن ماهو في النسيج الطبيعي وكانت احسن قيمة له (2.940 ) كدليل على ان الجزيئة تغيرت عن قيمته الطبيعية ,وكانت احسن قيمة له (4.156) في حالات انتشار السرطان في العقد اللمفاوية ,وقيمة (6.340 ) كدليل على المراحل المتقدمة من سرطان الثدي .بينما الموروث ( PTEN) كان في جميع الحالات منخفض عن النسيج الطبيعي واحسن قيمة له (0.210) كدليل لتغير تعبيرالموروث عن النسيج الطبيعي , وكانت القيمة) 0.175) دليل انتشاره الى الغدد اللمفاوية, وقيمة (0.098) تمثل المراحل المتقدمة من سرطان الثدي . هنالك علاقه عكسية بين جزيئة ال( miR - 21) والموروث ( PTEN) ولايوجد اي علاقة احصائية بين هذه المتغيرات وبين بقية عوامل التنبؤ الاخرى المعروفة في سرطان الثدي . الاستنتاجات : - التغير في التعبير كل من جزيئة ال ( miR - 21) والموروث (PTEN ) في جميع حالات سرطان الثدي وهنالك علاقة احصائية مع انتشار المرض الى العقد اللمفاوية ومع المراحل المتقدمة من سرطان الثدي . هنالك علاقة احصائية عكسية بين ازدياد التعبير للجزيئة (miR - 21) وانخفاض التعبيرللموروث (PTEN) في عينات سرطان الثدي . | Background : - Breast cancer is the most common cancer in women in worldwide. The phosphatase and tensin homolog gene is a tumor suppressor gene, and a key negatively regulator of cell signaling pathways that regulate growth and survival signaling pathways . More recently, microRNAs are small non protein coding RNAs involved in gene regulation. MicroRNA - 21 was one of the first oncogenic microRNAs and as an anti - apoptotic factor, to be characterized, being up - regulated in numerous tumors including breast cancer. The phosphatase and tensin homolog gene is one of microRNA - 21target genes Aim of the study : - To assess the validity of microRNA - 21 and phosphatase and tensin homolog (PTEN) gene expression as a diagnostic tool for gene alteration .Patients and Methods : - A prospective study , from January 2013 to January 2015. Fifty - pairs of fresh tissues from both breast cancer of invasive ductal carcinoma "NOS" and apparently normal adjacent tissues (from modify radical mastectomy) were by patients were recruited at the Surgical Department /Al - Diawania Teaching Hospital in Al - Diawania city . Total RNA extraction and real - time quantitative polymerize chain reaction technique were used for assessment of microRNA - 21 and phosphatase and tensin homolog gene expression. Tissue sample present in the paraffin embedded blocks belonging to tumor and normal adjacent tissue were used for Immunohistochemistry staining for (estrogen receptor, progesterone receptor and human epidermal growth factor receptor - 2) and dual color - chromogenic insitu hybridization technique for positive human epidermal growth factor receptor - 2 by Immunohistochemistry.Results : - Majority of cases 48(96%) ,were up regulated of microRNA - 21, indicating cancer tissue fold change of microRNA - 21 was significantly higher than that of normal adjacent breast tissue, and the best cutoff value for microRNA - 21 fold change in breast cancer tissues was (2.940)for diagnosis of gene alteration , (≥ 4.156) for positive lymph node involvement and (≥6.340) for higher stage (III,VI).All patients 50(100%) exhibit phosphatase and tensin homolog gene expression down regulation, indicating fold change of cancer tissue for phosphatase and tensin homolog gene expression was significantly lower than that of normal adjacent tissue and the best cutoff value for gene expressional alteration in breast cancer tissues was (0.210) for diagnosis of gene alteration, (≤ 0.175) for positive lymph node involvement and ( ≤ 0.098) for higher stage (III,VI) .Up - regulation of microRNA - A21 and down regulation of phosphatase and tensin homolog gene expression show no significantly correlate with other patients criteria like (age , grade ,size of tumor ,( estrogen receptor, progesterone receptor and human epidermal growth factor receptor - 2) by Immunohistochemical technique and human epidermal growth factor receptor - 2 gene amplification by dual color - chromogenic insitu hybridization technique . Identified significant negative correlation of up - regulation microRNA - 21 and down regulation of phosphatase and tensin homolog gene expression in breast cancer tissues.Conclusion : - gene expression of both microRNA - 21 and phosphatase and tensin homolog was significantly alterated in breast cancer tissues . It has been associated with positive lymph node involvement and higher tumor stage (III,VI)

خلل الوظائف اللاارادية في مرضى الشلل الرعاشي == DYSAUTONOMIA IN PARKINSON'S DISEASE

اسم المؤلف: اسماء خلف حمود
اسم المشرف: اسراء فائق جعفر | فرقد بدر حمدان
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الفسلجة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يؤثر على كبار السن بشكل اساسي، ولكن تتاثر الفئة العمرية الشابة ايضا. انه ينتج عن موت او تحلل الخلايا العصبية المنتجة للدوبامين بشكل رئيسي في المادة السوداء وقد يمتد الى اجزاء اخرى من الدماغ تحتوي على هذه الخلايا العصبية . ان السبب الرئيسي لهذا المرض لا يزال غير معروف، على الرغم من ان هناك العديد من العوامل التي تساهم في حدوثه.احد هذه العوامل هو الالتهاب، الاكسدة، واختلال عمل بيوت الطاقة (الميتوكوندريا) بالاضافة الى الاستعداد الوراثي للمريض المصاب بهذا المرض.ان الاعراض المرتبطة بمرض باركنسون هي الاعراض الحركية ، غير الحركية وغيرها من الاعراض بما في ذلك اعراض اختلال الجهاز العصبي اللاارادي.تربط هذه الدراسة بين اعتلال الجهاز العصبي اللاارادي من مرض باركنسون مع مواد استيل كولين، ومضادات الاكسدة، وعامل نخر الورم في ثلاث مجموعات دراسية التي هي مرضى باركنسون مع خلل الوظائف التلقائية (اللاارادية)، ومرضى باركنسون دون خلل الوظائف التلقائية ومجموعات الاشخاص الاصحاء.يتم ذلك من خلال دراسة الفحوصات اللاارادية بما في ذلك فحوصات القلب والاوعية الدموية اللاارادية (نسبة فالسالفا، ونسبة 30 : 15، والتغير في ضغط الدم عند الوقوف)، واستجابة الجلد السمبثاوي (SSR)، بالاضافة الى اختبار الكيمياء الحيوية من مستويات الاستيل كولين، ومضادات الاكسدة، وعامل نخر الورم.فيما يتعلق الاختبارات اللاارادية، اظهرت النتائج ان التغيرات في ضغط الدم عند الوقوف كان لها دلالة احصائية بالاضافة الى ذلك، نقص ضغط الدم الوضعي يمثل اهم اعراض خلل الوظائف التلقائية في مرض باركنسون.اما بالنسبة لمستوى الاستيل كولين فقد انخفض مع تطور مرض الشلل الرعاشي وذلك باصابة جهاز الوظائف التلقائية (اللاارادية). اضافة لذلك فان مستوى مضادات الاكسدة لم يظهر اي دلالة احصائية.من ناحية اخرى، نسبة عامل نخر الورم يزداد بتتفاقم المرض ليشمل خلل الوظائف التلقائية اللاارادية.بينت الدراسة ان عامل نخر الورم وعامل شدة الاكسدة يؤثران على تقدم مرض باركنسون ككل وخلل الوظائف الاارادية لهذا المرض بشكل خاص. | Parkinson disease is regarded as a common neurodegenerative disorder in the whole world that affects mainly elderly people, but young age group are also affected. It results from dopaminergic neuronal degeneration mainly in substantia nigra that may extend to other parts of the brain. The main cause of this disorder still unknown, although many factors that contributes in its pathogenesis. Of these factors, there are inflammation, oxidative stress, and mitochondrial dysfunction in addition to genetic predisposition.Symptoms related to Parkinson’s disease are motor, non - motor. The present study relates the autonomic dysfunction of Parkinson disease with the acetylcholine, antioxidants, and tumor necrosis factor in three study groups which are Parkinson patients with autonomic dysfunction, Parkinson patients without autonomic dysfunction and the control groups. The autonomic assessment is done by studying the autonomic tests including cardiovascular autonomic study (Valsalva ratio, 30 : 15 ratio, blood pressure change with posture), and sympathetic skin response, in addition to biochemical testing of acetylcholine, antioxidant, and Tumor necrosis factor levels. Regarding autonomic testing, the results difference that is statistically significant regarding changes of blood pressure in response to posture, in addition Parkinson disease. Acetylcholine level decrease as the disease progress from Parkinson patients without autonomic dysfunction to Parkinson patients with autonomic dysfunction. While antioxidant level was not significant difference between Parkinson patients with and without dysautonomia. On the other hand, tumor necrosis factor increases as the disease worsen to involve autonomic dysfunction. This study shows the effect of tumor necrosis factor and oxidative stress affecting the pathogenesis of Parkinson disease as a whole and autonomic dysfunction in specific

استهداف الخلية السرطانية بواسطة نانو الهجين الحويصلي كنهج جديد لموت الخلايا المبرمج == Targeting Cancer Cell by Nano Hybrid Liposome : A new Approach for apoptosis

اسم المؤلف: نورا عبد عواد
اسم المشرف: قسمة محمد تركي | ناهي يوسف ياسين
الموضوع العام: الطب
السنة: 2015
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: الخلفية : يعتبر العلاج الهادف للسرطان بالنانو حقلا مهما للبحث عن علاجات خاصة بالسرطان لتجنب الاعراض الجانبيه للادويه الكيمياويه التقليديه ،ولان خلايا السرطان تعاني من عطل في منظومة الموت المبرمج يؤدي هذا الى تفضيلهه لمسارات البقاء على قيد الحياة ومقاومة الادويه ولهذا يعتبر تحفيز الموت المبرمج في الخلايا السرطانية من الوسائل المفضلة للتطبيق في اي دواء مستحدث لعلاج السرطان .ان اهداف العلاج بالنانو هو رفع الكفاءة العلاجية للدواء وانتقائيتة والتغلب على مقاومة الخلايا له والتي تمثل عقبة في علاج السرطان.وقد يفيد استعمال الاجسام النانويه الدهنية المهجنة بكل هذه الاغراض في علاج السرطان .تتكون الاجسام النانوية الدهنية المهجنه من مع الايثر)C12(EO)23)L - DMPC والذي يتداخل انتقائيا مع جدار الخلية السرطانية مؤديا الى موتهاالاهداف : تقييم قابلية اجسام النانو الدهنيه المهجنه والمصنعه محليا على احباط نمو خلايا سرطان عنق الرحم والرابدومايوساركوما كتاكيد لحصول الموت المبرمج في زراعتها المحضونه مع الهجين الحويصلي المصنع مختبريا.طرق العمل حضرت اجسام النانو الدهنية الهجينة من مزج [DMPC & C12(E0)23] ومزجها في وسط زررعي خاص ثم بعد ذلك يتم وضعها في حمام مائي لامواج فوق الصوتية Sonicatore) ) للحصول على الشكل الكروي والحجم النانوي الهجين وللتاكد من ذلك تم فحصها باستخدام المجهر الالكتروني الماسح (SEM) ومجهر القوة الذرية (AFM) , وتم تحديد حيوية الخلايا عن طريق احتساب نسه الخلايا الميتة الى الحية بعد تصبيغهه بالصبغات التي تبين سمية الخلايا Crystal Violate&MTTوتم فحص والتاكد من الموت المبرمج بتقنيات متعدده منها الصبغات المتفلوره واختلال شدة نفاذية المايتوكوندريا وقياس فعاليه انزيم PCR وال, (Caspase - 3) النتائج : النتائج تمكن الهجين الحويصلي الواقع ي مجال وانتقائية النانو من احباط انقسام خلايا سرطاني عنق الرحم، والرابدومايوساركوما وبتركيز نصفي (IC50) مساوي لــ (0.2 mmol)، بينما لم تتاثر الخلايا اللمفاوية الطبيعية بهذه الظروف.وكان التاثير واضحا في كل الطرق التي استعملت لقياس الموت المبرمج وقدمت ادله واضحة على ان الهجين الحويصلي قادر على تحفيز الموت المبرمج في خلايا سرطاني عنق الرحم والرابدمايوساركوما عند حضنها مع الجرعه المحبطه النصفية للهجين الحويصلي ولمدة 24 ساعه .الاستنتاجات : يمكن اعتبار الهجين الحويصلي المصنع كوسيلة ممكنه للعلاج بالنانو وباقل اضرار جانبيه للخلايا الطبيعيه مقارنة مع العلاجات المستعمله الاخرى. وتوصي هذه الدراسة ببحث طريقة عمل الهجين الحويصلي وقابليته على احباط نمو انواع اخرى من الخلايا السرطانيه في المختبر وفي البحوث الحيه | Background : Cancer targeted nanotherapy represent an exciting field in the search for new cancer specific therapies to avoid conventional chemotherapy side effects. Because cancer cells usually have malfunctioning apoptotic machinery which favors survival pathways and drug resistance, cancer cell apoptosis is the favorable event to be induced in any new anticancer agent development. Nanotherapy goals are to elevate therapeutic efficiency, selectivity, and overcome drug resistance as major obstacle in cancer treatment. Hybrid nanoliposomes (nHLs) may fulfill all these features in cancer therapeutics.Hybridnanoliposomes (HLs) composed of L - α - dimyristoylphosphatidylcholine (DMPC) and Polyoxyethylene (23) dodecyl ether (C12 (EO)23) can integrate selectively into the cancer cell membrane inducing cancer cell death.Objectives : to assesses the capacity of locally synthesized hybrid nanoliposome to inhibit the growth of cervix cancer cells (HeLa) andrabdomyosarcoma(RD)to confirm the event of apoptosis in HeLa and RD cell lines incubated with in - house synthesized and characterized nHLs.Methods : hybrid nanoliposomes(nHLs) synthesized by sonication method from a mixture of DMPC and C12(EO)23 in tissue culture media RPMI - 1640 for 6 hours at 300W and 40ºC then filtration with 0.2µm filter. Shape and size characterized with scanning electron microscope (SEM) and atomic force microscope(AFM). Viability of HeLacell,RD cell, and normal lymphocytes challenged with HLs were determined using MTT and Crystal violate assay. Induction of apoptosis in the challenged cells was examined by staining with fluorescence dye mix cridine orange/propidium iodide,cell gel electrophoresis, mitochondrial membrane potential disruption, caspase - 3 activity and real time PCR.Results : synthesized nHLs were in nanozise range and selectively inhibited HeLa cells proliferation with IC50 of 0.2mM with no effect against normal lymphocytes. Apoptosis was evident ofHeLa cells population and RD was evident of treated with nHLs.All apoptosis detection procedures used gave a clear defined significant indication that nHLs was capable of inducing apoptosis in HeLa and RD cells incubated with the IC50.Conclusion : synthesized nHLs may considered as promising nanotherapy, this study recommends further inspections for the mechanism of action of nHLs and there capabilities to inhibit other types of cancers both invitro and in vivo.

التعبير المناعي النسيجي الكيميائي لبروتينات (CD24, CD44 وALDH1) للخلايا الجذعية السرطانية في سرطان الثدي : دراسة سريرية مرضية == Immunohistochemical Expression of Stem Cell Markers CD24, CD44 and ALDH1 in Invasive Breast Carcinoma A Clinicopathological Study

اسم المؤلف: حسنين غسان حسين
اسم المشرف: سالم رشيد حمودي العبيدي
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الامراض النسيجية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعد سرطان الثدي واحدا من الاورام السرطانية الاكثر شيوعا في النساء بالعالم يوقدر بحوالي 1,7 مليون حالة جديدة مسجلة في عام 2012 (بنسبة 11,9% من باقي الاورام السرطانية). في العراق سرطان الثدي يعتبر الاكثر شيوعا لدى النساء ويشكل ما نسبته 34,8% من بين باقي الاورام السرطانية (سجلات وزارة الصحة). الخلايا الجذعية السرطانية تعرف بانها : مجموعة صغيرة من الخلايا السرطانية داخل الورم السرطاني تعتبر المخزون لاعادة تكاثر الخلايا السرطانية وتكوين تنوع خلوي ذات تخصص مختلف في مراحل نموها, من المؤشرات المقبولة للخلايا الجذعية السرطانية هي : CD24 , CD44 , CD133 , EPCAM وALDH1).الهدف من الدراسة : تقييم التعبير المناعي النسيجي الكيميائي لمؤشرات الخلايا الجذعية السرطانية (CD24 , CD44 وALDH1) في كل من النسيج الطبيعي للثدي , الورم الغدي الليفي الحميد في الثدي وسرطان الثدي (بنوعيه سرطان الثدي القناتي الاختراقي وسرطان الثدي الفصي الاختراقي) وعلاقة هذا التعبير المناعي النسيجي الكيميائي مع المتغيرات السريرية المرضية (العمر , حجم الورم السرطاني , درجة التمايز للورم السرطاني وحالة الغدد اللمفاوية).المرضى , المواد وطرق العمل : من تاريخ نيسان 2012 الى اذار 2013 , تم اخذ 133 عينة نسيجية تشمل 37 عينة نسيجية للمجموعة الغير مصابة بالورم السرطاني ( 12 عينة لنسيج طبيعي للثدي و25 عينة للورم الحميد ) و96 عينة نسيجية للمجموعة المصابة بالورم السرطاني ( تتالف من 51 حالة رفع ثدي كامل و45 عملية استئصال للورم) حيث تكونت من 87 حالة من سرطان الثدي القناتي الاختراقي وتسع حالات من سرطان الثدي الفصي الاختراقي. اخذت الانسجة الارشيفية المطمورة في شمع البارافين للمدة بين 2011 و2013 من ملفات المختبرات التعليمية لدائرة مدينة الطب ومختبر الغدق التخصصي الخاص ومن معهد الطب العدلي في بغداد. وكل المعلومات السريرية تم استحصالها من البيانات الخاصة بالمرضى. كل العينات اعيد تقيمها بصبغة الهيماتوكسلين والايوسين وتم التحري عنها بواسطة الصبغة المناعية النسيجية الكيميائية للمؤشرات (CD24 , CD44 وALDH1).النتائج : وجد انه هناك علاقة ذات دلالة احصائية بين الحالة الصحية للثدي (ان كان الثدي طبيعي , به ورم حميد او ذات ورم سرطاني) والتعبير المناعي النسيجي الكيميائي للمؤشرات الثلاثة للخلية الجذعية السرطانية (CD24, CD44 وALDH1).التعبير المناعي النسيجي الكيميائي لمؤشر CD24 في نسيج الثدي : عشرة حالات من اصل 37(27%) للمجموعة الغير مصابة بالورم السرطاني يكون التعبير المناعي موجب. بالنسبة للمجموعة المصابة بالورم السرطاني , 41 حالة من اصل 87(47,1%) من حالات سرطان الثدي القناتي الاختراقي واربع حالات من اصل 9(44,4%) من حالات سرطان الثدي الفصي الاختراقي يكون التعبير المناعي موجب. ولم تكون العلاقة ذات دلالة احصائية بين مؤشر (CD24) والمتغيرات السريرية المرضية (العمر, حجم الورم, درجة التمايز وحالة الغدد اللمفاوية).التعبير المناعي النسيجي الكيميائي لمؤشر CD44 في نسيج الثدي : ثمانية حالات من اصل 37(21,6%) للمجموعة الغير مصابة بالورم السرطاني يكون التعبير المناعي موجب. اما ما يخص المجموعة المصابة بالورم السرطاني, 27 حالة من اصل 87(31,03%) من حالات سرطان الثدي القناتي الاختراقي وثلاث حالات من اصل 9(33,3%) من حالات سرطان الثدي الفصي الاختراقي يكون التعبير المناعي النسيجي موجب. توجد علاقة ذات دلالة احصائية بين مؤشر CD44 للخلايا الجذعية السرطانية مع حالة الغدد اللمفاوية حيث كانت قيمة (P) تساوي (0,0140). ولم يكن هنالك علاقة ذات دلالة احصائية بين مؤشر (CD44) وباقي المتغيرات السريرية المرضية (العمر, حجم الورم ودرجة التمايز).التعبير المناعي النسيجي الكيميائي لمؤشر ALDH1 في نسيج الثدي ينقسم الى : 1. التعبير المناعي لمؤشر ALDH1 في نسيج الخلايا السرطانية : سبعة حالات من اصل 37(18,9%) للمجموعة الغير مصابة بالورم السرطاني يكون التعبير المناعي موجب. وفيما يخص المجموعة المصابة بالورم السرطاني, 34 حالة من اصل 87(39,2%) من حالات سرطان الثدي القناتي الاختراقي وثلاث حالات من اصل 9(33,3%) من حالات سرطان الثدي الفصي الاختراقي يكون التعبير المناعي موجب. توجد علاقة ذات دلالة احصائية بين مؤشر ALDH1 للخلايا الجذعية السرطانية مع حالة درجة تمايز الورم حيث كانت قيمة (P) تساوي (0,0323). ولم يكن هنالك علاقة ذات دلالة احصائية بين مؤشر ALDH1 وباقي المتغيرات السريرية المرضية (العمر, حجم الورم وحالة الغدد اللمفاوية).2. التعبير المناعي لمؤشر ALDH1 في النسيج المحيط للخلايا السرطانية : ثمانية حالات من اصل 37(21,6%) للمجموعة الغير مصابة بالورم السرطاني يكون التعبير المناعي متوسط الى قوي التعبير في الخلايا المحيطة للخلايا السرطانية و29(78,4%) حالة يكون التعبير المناعي منعدم الى ضعيف في هذه الخلايا. اما ما يخص مجموعة المصابة بالورم السرطاني للثدي, 47 حالة من اصل 87(54,2%)من حالات سرطان الثدي القناتي الاختراقي وخمس حالات من اصل 9(55,6%) من حالات سرطان الثدي الفصي الاختراقي يكون التعبير متوسط الى قوي. من هذه الدراسة وجد انه لايوجد علاقة ذات دلالة احصائية بين التعبير المناعي لمؤشر ALDH1 في النسيج المحيط للخلايا السرطانية والمتغيرات السريرية المرضية (العمر, حجم الورم, درجة التمايز وحالة الغدد اللمفاوية).الاستنتاج : - توجد علاقة ذات دلالة احصائية بين مجموعة الغير مصابة بالورم السرطاني والمجموعة المصابة بالورم السرطاني للثدي من حيث التعبير المناعي للمؤشرات (CD24, CD44 وALDH1) في الخلايا الجذعية السرطانية. - هنالك علاقة ذات دلالة احصائية بين فرط التعبير المناعي لمؤشر CD44 وحالة الغدد اللمفاوية في حالات سرطان الثدي. - في هذه الدراسة توجد علاقة ذات دلالة احصائية بين فرط التعبير المناعي لمؤشر ALDH1 في الخلايا السرطانية ودرجة التمايز في الورم السرطاني للثدي. - نتائج المتحصلة من هذه الدراسة تقترح بان فرط التعبير المناعي النسيجي الكيميائي للمؤشرات المناعية (CD24, CD44 وALDH1) قد ترتبط بحالة تحول الورم الى النوع السرطاني وكذلك بالحالة البايولوجية لسرطان الثدي. | Breast Cancer is the most frequent diagnosed cancer among women worldwide with an estimated 1.7 million new cancer cases diagnosed in 2012 (11.9% of all cancers). In Iraq, cancer of the breast is the commonest cancer in females, in 2010 constituted 34.8% of all other malignancies in women. Cancer stem cells have been defined as - a small subset of cancer cells within a cancer that constitute a reservoir of self - sustaining cells with the exclusive ability to self - renew and to cause the heterogeneous lineages of cancer cells that comprise the tumor, the well - accepted cancer stem cell markers are CD44, CD24, CD133, CD166, EpCAM and ALDH1.Aim of the studyTo assess the immunohistochemical expression of CD24, CD44 and ALDH1 as stem cell markers in normal breast tissue, fibroadenoma and breast carcinoma (Invasive ductal and Invasive lobular carcinomas), in correlation with some clinicopathological parameters (Age of patient, tumor size, tumor grade and lymph node status).Patients, materials and methods From April 2012 to March 2013, total of (133) tissue samples of surgical breast biopsies were included in this retrospective study, 37 cases of non - malignant breast cases (12 cases of normal breast tissue and 25 fibroadenoma case) and 96 cases of malignant breast cases (51 mastectomy case and 45 cases of excisional biopsies) were all of them invasive breast carcinoma, eighty seven cases were of invasive ductal carcinomas and nine cases of invasive lobular carcinomas. The samples were obtained from archival paraffin embedded blocks for the years (2011) to (2013) from the histopathology files of department of pathology in Teaching Laboratories of medical city Hospital, Al - Ghadaq’s private Laboratory and Institute Of Forensic Medicine in Baghdad.. The histopathological diagnosis had been revised and all cases were stained by immunohistochemical technique with CD24, CD44 and ALDH1 tumor markers.Results There was significant difference among the three states of health status (normal breast cases, fibroadenoma cases and invasive breast carcinoma) and immunohistochemical expression of stem cell marker (CD24, CD44 and ALDH1 epithelial and stromal expression). Regarding Immunohistochemical expression of CD24, ten cases out of 37(27%) of non malignant breast cases were positive of CD24 expression. For malignant breast cases, forty one invasive ductal carcinoma cases out of 87(47.1%) and four invasive lobular carcinoma cases out of 9(44.4%) were CD24 with positive expression, and there was no significant difference regarding CD24 expression in relation to different clinicopathological variables (age of the patient, tumor grade, lymph node status and tumor size). For CD44 immunohistochemical expression, eight cases out of 37(21.6%) of non malignant breast cases were positive of CD44 expression. For malignant breast cases, Twenty seven invasive ductal carcinoma cases out of 87(31.03%) and three invasive lobular carcinoma cases out of 9(33.3%) were positive CD44 expression, and there was a significant difference regarding CD44 expression in relation to lymph node status of the patients (P=0.0140). Regarding the age of the patient in invasive breast carcinomas there was no significant difference between CD44 expression and the three groups of the patient age, so as for grade of tumor and tumor size (P > 0.05). Regarding ALDH1 epithelial immunohistochemical expression, seven cases out of 37(18.9%) of non malignant breast cases were positive of CD44 expression, while for malignant breast cases, thirty four invasive ductal carcinoma cases out of 87(39.2%) and three invasive lobular carcinoma case out of 9(33.3%) showed a positive expression of epithelial ALDH1. There was no significant difference in epithelial ALDH1 expression between different age groups of the patients. CD44 immunohistochemical expression was significantly different in different grades of invasive breast carcinomas (P=0.0323). The mean value of ALDH1 - E expression showed no significant difference between cases with positive lymph node involvement and those with a negative lymph node involvement, also there was no significant correlation in epithelial ALDH1 immunohistochemical expression in relation to the tumor size. For stromal ALDH1 immunohistochemical expression, eight cases out of 37(21.6%) of non malignant breast cases were moderate/strong of stromal ALDH1 expression and 29(78.4%) cases were none/weak expression. Regarding malignant breast cases, forty seven invasive ductal carcinoma cases out of 87(54.2%) and five invasive lobular carcinoma cases out of 9(55.6%) were moderate/strong expression of stromal ALDH1, and there was no significant difference regarding stromal expression of ALDH1 in relation to different clinicopathological variables (age of the patient, tumor grade, lymph node status and tumor size). Conclusions • A statistical significant correlation has been found between Malignant Breast cases and Non Malignant Breast cases regarding the Immunohistochemical over expression of stem cell markers (CD24, CD44 and ALDH1 epithelial and stromal Expression).• There was a statistical significant correlation between CD44 immunohistochemical expression and lymph node status in invasive breast carcinomas cases.• A statistical significant correlation has been found between ALDH1 Epithelial immunohistochemical expression and Grade of tumor in cases of invasive breast carcinoma. • The results of this study suggest that higher stem cell markers (CD24, CD44 and ALDH1) expression may be associated with malignant transformation and progression in breast cancer biology

الكشف الجزيئي عن فايروس الجي سي وجينات البي ثلاثة وخمسون والشبكية الجيني والبتا - كتنين في المرضى المصابين بالاورام الغدية للقولون والمستقيم == MOLECULAR DETECTION OF JC VIRUS, P53, RETINOBLASTOMA GENES AND ? - CATENIN IN PATIENT WITH COLORECTAL ADENOCARCINOMA

اسم المؤلف: ابراهيم فاضل ابراهيم الدروبي
اسم المشرف: فائزة عبد الله مخلص | بان عباس عبد المجيد
الموضوع العام: الطب
السنة: 2015
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تمهيد : يعود فايروس الجي سي الى عائله الفيروسة التوارمية. ينتشر الفايروس بين السكان وعلى نطاق واسع حول العالم. يحمل الفايروس جينة Ag T والتي لها القدره على التكوين السرطاني في الانسجة من خلال التفاعل مع المسارات التنظيمية المختلفه, ويمكن ان تتداخل مع التحكم في دوره الخلية واليات عدم الاستقرار الجيني.العلاقة بين فايروس الجي سي وسرطان القولون وسرطان المستقيم لايزال المنظر ينتضرالقرار النهائي.اهداف الدراسة : 1. تحد يد الدور المحتمل لفايروس الجي سي في سرطان القولون والمستقيم من خلال الكشف والتقدير الكمي لجينة التي وتحميل جينة الاكنوبروتين واظهار الحامض النووي الدي ان اي للفايروس في كل من الخزعات النسيجية السرطانية والطبيعية للقولون والمستقيم. 2. الكشف عن الايجابية المصلية من خلال فحص الاجسام المضادة للفايروس في مصول مجموعه البحث.3. تقدير الرابطة المحتملة بين فايروس الجي سي مع البيتاكاتانين وملاحظة قابلية الفايروس على كبح او تحديد عمل الجينات المشبطة للاورم البي خمسة وثلاثون والشبكية الجيني.طرق العمل : تم تصميم البحث كدراسة مستقبلية للسيطرة على الحالة وانطوت على اخذ الجزعات الطازجة لانسجة القولون والمستقيم من خلال تنظير القولون والمستقيم وقد شملت على 28 مريض مصاب بورم القولون والمستقيم الخبيث وثلاث وثلاثون مريض ممن لم يشخص لديهم ورم سرطاني قي القولون والمستقيم والذين راجعوا وحدة التنظير في مستشفى الاورام ومستشفى بغداد التعليمي ومستشفى اليرموك التعليمي خلال الفتره من حزيران 2013 ولغاية اذار2014. تم الكشف عن جينة فايروس الجي سي ( التي جين) والاكنوبروتين بواسطة جهاز تفاعل سلسلة البلمره وكذلك فحص الحامض النووي الدي ان اي لفايروس الجي سي من خلال التهجين الموقعي وكذلك تم الكشف عن الاجسام المصلية المضادة للفايروس بطريقة الاليزا. تم الكشف عن البيتا - كتنين وبروتين الشبكية الجيني بطريقة المعلمات المناعية والكشف عن البي خمسة وثلاثون بتقنية التهجين الموقعي الفلوري.النتائج• بالنسبة لمجموعة السرطان كان اعلى نسبة تردد للاصابة بورم القولون والمستقيم (35.71%) في المجموعة العمرية مابين 50 - 59 سنة. كان سرطان القولون والمستقيم اكثر شيوعا في الذكور منه في الاناث وبنسبة 1.8 : 1.• اضهر الفحص النسيجي ان النوع الاكثر شيوعا كان بنسبة 85.72% من نوع غدية غير ميوسينية.وان ورم القولون الاكثر شيوعا هو غدية متباينة بشكل معتدل (75%) بين درجات غدية ورم القولون والمستقيم• تم الكشف عن جينة الورم لفايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل وسجل اعلى تردد للاصابة(57.1%) واعلى نسبة نسخ من الجين في مجموعة السرطان مقارنته بمجموعة السيطرة (27.3%) وكانت نتائج الحمل الفايروسي لمجموعة السرطان بمعدل416.93±217.77 نسخة/مايكروغرام اما مجموعة السيطرة فكانت بمعدل229.866±111.49 نسخ /ميكروغرام والتي اضهرت بدورها فرق كبير بين مجموعات الدراسة.• تم تشخيص جينة الاكنوبروتين لفايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل في مجموعة السرطان وكانت النتسبة ( 53.6%) وهي اعلى من النسبة التي تم تشخيصها في مجموعة السيطرة والتي كانت (15.2%). الحمل الفايروسي في مجموعة السرطان كان بمعدل 317±129.12 نسخة/ مايكروغرام وهو اعلى من معدل الحمل الفايروسي في مجموعة السيطرة (152.94±10514) نسخة/ مايكروغرام .• وجد اتفاق كبير بين نتائج تشخيص جينة التي وجينة الاكنوبروتين بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل لمجموعة السرطان ومجموعة السيطرة.• بطريقة التهجين الموقعي لل(دنا) لفيروس الجي سي, اضهرت النتائج ان 53.6% من مجموعة السرطان اضهرو نتائج موجبة لفايروس الجي سي . اما مجموعة السيطرة فاضهرت (30.3%). وكان الفروق ذات دلاله احصائية بين مجموعاتا لدراسة P<0.05 . تبين وجود فروق ذات دلالة احصائية بين مجموعات الدراسة بشان كثافة اشارة ونسبة الاصابة ونمط تورط (P <0.05)، في حين لا توجد فروق كبيرة عثر فيما يتعلق بالمشاركة غدي او اللحمية (P = 0.458)• لم يتم العثورعلاقة ذات دلالة احصائية عند مقارنة ال (دنا ) بواسطة التهجين الموقعي وفقا لدرجات ومواقع الورم.• وجد اتفاق كبير بين نتائج تشخيص جينة التي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج التشخيص بطريقة التهجين الموقعي لل ( دنا) بين مجموعات الدراسة.• وجد اتفاق متوسط بين نتائج تشخيص جينة الاكنوبروتين بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج تشخيص فايروس الجي سي بطريقة التهجين الموقعي لل (دنا) بين مجموعات الدراسة.• فحص المضاد المناعي لفايروس الجي سي بطريقة الاليزا اضهرت وجود مضاد مناعي في 14/ 22(63.6%) مريض من مجموعة السرطان في حين وجد ان 15/28 (53.6%) من مجموعة السيطرة تحمل المضاد المناعي. (p=0.474) وجد توافق ضعيف بين نتائج تشخيص فايروس الجي سي بطريقة الوقت الحقيقي لتفاعل البلمرة المتسلسل ونتائج التهجين الموقعي لل (دنا) ونتائج فحص المضاد المناعي في مجموعات الدراسة.• تم الكشف عن توطين البتا كتنين في النووية بطريقة الفحص النسيجي المناعي الكيمياوي وكانت بنسبة12/28 ( 42.86%) من مجموعة السرطان، في حين لم يكشفت على تلطيخ نووي ايجابي في مجموعة السيطرة (P <0.05). لم يتم العثور على علاقة احصائية لا مع درجات الوروم ولا الى موقع الورم.• تم الكشف عن بروتين جينة الشبكية الجيني بتقنية الفحص النسيجي المناعي الكيمياوي.بالنسبة لمجموعة السرطان 10/28 (35.7%) اضهرت تلطيخ نووي ايجابي في حين ان 18/28 (64.3٪) كشفت عن تلطيخ نووي سلبي اما مجموعة السيطره فاضهرت(100%) تلطيخ ننووي ايجابي. لا اتفاق بين نتائج فحص جين الرتينوبلاستوما وجينة التي وجينة الاكنوبروتين وكذلك التهجين الموقعي لل (دنا) بين مجموعات الدراسة.• بطريقة التهجين الموقعي الفلوري تم الكشف عن التعبير الجيني للبي خمسة وثلاثون. فوجد ان خمسة حالات تم شطب موقع البي خمسة وثلاثون من كرومسوم رقم 17. ثلاث حالات وجدت فيها شطب كروموسوم 17 وحالة واحد وجد فيها خليط من شطب موقع البي خمسة وثلاثون وايضا شطب لكروموسوم رقم 17.الاستنتاجاتابرزت هذه الدراسة الدور المحتمل للفايروس الجي سي التي قد تشارك في طرق مختلفة في التسبب في سرطان القولون والمستقيم، والتي تتفق مع دراسات اخرى. على الرغم من هذه الادلة دور JCV في الاورام الخبيثة القولون والمستقيم وoncoproteins في تعزيز التحول لا يزال بعيدا عن الوضوح. | John Cunningham Virus (JCV) a member Polyomaviridae family is a type of human polyomavirus. It’s widespread virus detected in different populations throughout the world. JCV encodes for T - Ag which have oncogenic capability through interaction with different regulatory pathways and can interfere with cell cycle control and genomic instability mechanisms. The association between JCV and colorectal carcinoma still awaits the final conformation. Aims of study : 1. Determine the possible role of JCV in colorectal carcinoma by detection, quantification of T - Ag gene and agnoprotein gene load and demonstrating JCV DNA in both colorectal carcinoma and normal colonic tissue biopsies. 2. Detect the seropositivity of JCV antibodies among the study groups.3. Estimate the possible interaction of T - Ag with B - catenin and P53 and retinoblastoma tumor suppressor genes. Methods : This study was designed as prospective case - control study that involved fresh colonic tissue biopsies taken through colonoscopy from 28 patients with colorectal cancer and from 33 patients who did not have colorectal carcinoma attending to GIT endoscopic unit of Oncology Hospital, Baghdad Teaching Hospital, and Al - Yarmouk Teaching Hospital during the period from June 2013 to march 2014. JCV DNA was detected and quantified by real time PCR for T - Ag gene and agnoprotein genes. Using JCV bioprobe, chromogenic in situ hybridization (CISH) technique was used to detect JCV in tissue biopsies. Serological detection of JCV IgG antibodies was detected by ELISA. B - catenin and retinoblastoma protein products were detected by immunohistochemistry. Tumor suppressor gene P53 was detected by fluorescent in situ hybridization technique. Results : • Among the total 28 colorectal carcinoma cases, the highest frequency 10/28(35.7%) were detected in age group 50 - 59 years and its more frequent in male than female with ratio 1.8 : 1.• Histopathological finding revealed that 24(85.72%) cases were non mucinous adenocarcinoma and moderately differentiated adenocarcinoma was more frequent 21/28 (75%) among grades of CRC.• JCV T - Ag was detected in 16 (57.1%) of CRC group and 9/33(27.3%) of control group, with viral load VL mean 416.93 ± 217.77 copy/µg for CRC group and 229.866 ± 111.49 copy/µg for the control group which in turn showed significant difference between the study groups• Agnoprotein gene was detected in 12/28 (42.9%) of CRC group with VL mean (317 ± 129.12) compared to 5/33 (15.2%) with VL mean (152.94 ± 105.14) in non CRC patient (p=0.016). • Substantial agreement was found between T Ag gene and agnoprotein gene by real time PCR for both CRC group K=0.72 and control group K=0.784.• Using chromogenic in situ hybridization technique JCV DNA was detected in 15/28(53.6%) of CRC compared to 10/33(30.3%) in control group (p=0.065). Significant differences were detected comparing the percentage of JCV DNA in study groups according to intensity, score and signal pattern of involvement (p<0.05).• Substantial agreement was found between T Ag by qPCR and JCV DNA by CISH among the study groups. Moderate agreement was found between JCV agnoprotein gene by qPCR and JCV DNA by CISH among the study groups. • Serological detection of JCV antibodies by ELISA revealed 14/22 (63.6%), 15/28 (53.6%) of CRC group and control group showed positive results respectively. (p=0.474). Poor agreement was found between (T Ag by real time PCR and by CISH) and JCV antibodies by ELISA in the study groups.• Nuclear localization of B - catenin was detected by immunohistochemistry in 12/28(42.86%) of CRC groups, while none of the control group revealed positive nuclear staining (p<0.05). No significant associations found neither to grades nor to the site of tumor.• Retinoblastoma protein was detected by IHC. Only ten out of 28 (35.7%) showed positive nuclear staining, 18/28 (64.3%) of CRC revealed negative nuclear staining. All control group showed positive nuclear staining (p=0.000). No agreement was found between Rb protein and JCV T Ag, agnoprotein gene and JCV DNA by CISH among study groups.• P53 deletion was detected using FISH technique. Five cases out of 25 were with deleted p53 region, 3/25 were with deleted whole chromosome 17, and 1/25 revealed combination signals of deleted p53 region and deleted whole chromosome 17. The results were invalid for statistical analysis.Conclusion : This study highlighted the possible role of JCV which might participate in different ways in the pathogenesis of colorectal carcinoma, which in agreement with other studies. Despite these evidences the role of JCV in colorectal malignancies and its oncoproteins in promoting transformation is still far from clear

تقييم مستوى بعض السايتوكينات الدهنية والبروتين الدهني الواطي الكثافة المؤكسد ومستوى دهون الدم قبل وبعد استعمال الميثوتركسيت في مصل مرضى داء الصدفية : دراسة حالات مرضية - مجموعة ضبط == Evaluation of some serum adipokines, oxidized low density lipoprotein and lipid profile before and after methotrexate treatment of chronic plaque psoriasis (A case - control study)

اسم المؤلف: حيدر عبد جبار داود العمار
اسم المشرف: مناف صالح داود | محمد كاظم الحطاب
الموضوع العام: الطب
السنة: 2015
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعتبر داء الصدفية من الامراض الالتهابية الشائعة التي تصيب الجلد وهو ذاتي المناعة ويصيب الناس بمعدل (2 - 4)% من مجموع سكان العالم. خلايا T المخترقة لانسجة الجلد، الانترلوكينات، والسايتوكينات مثل visfatin)) ، وهرمونhigh molecular weight adiponectin - ذات اوزان جزيئية كبيرة ، وبروتينات رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الواطئ الكثافة المؤكسدox - LDL جميعها تؤثر بداء الصدفية. عادة يبدو جلد المريض بداء الصدفية مقشرا ومغلف بلون ابيض فضيا وممكن ان يصيب بقية اجزاء الجسم. يطرا على حياة المريض بداء الصدفية نفس ما يطرا على مرضى السكري من الدرجة الثانية او المرضى النفسيين او مرضى القلب من تغيرات. ويكون المريض بداء الصدفية بدينا مقارنة بعامة الناس. على الرغم من ان معرفتنا بسبب السمنة التي يصاب بها المريض بداء الصدفية الا ان هنالك الكثير من الدراسات التي وضعت بهذا الشان تفترض بان السبب هو افراز السايتوكينات المحفزة للالتهاب بواسطة الانسجة الشحمية ربما هو المسؤل عن تدهور حالة الصدفية. الدراسة الحالية قامت باجراء اختبارات لقياس التغيرات الحاصلة في مستويات انزيم الفيزفاتن ، وهرمون سايتوكين دهني ذات اوزان جزيئية كبيرة HMW - adiponectin، وبروتينات رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الوطئ الكثافة المؤكسدox - LDL وكذلك اقتران هكذا تغيرات مع مدى نشاط او استفحال المرض. الاهداف : ان هدف الدراسة هو : لمعرفة التغيرات الحاصلة في مستويات انزيم الفيزفاتنvisfatin ، السايتوكين الدهني(الاديبونكتين) ذات اوزان جزيئية كبيرةHMW - adiponectin ، وبروتينات رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الواطئ الكثافة المؤكسدoxidized - LDL في مصل مرضى داء الصدفية قبل وبعد العلاج بالميثوتراكسيت بالمقارنة بمستوياتها في مجموعة الضبط وكذلك لتقييم تاثير ذلك العلاج على مستويات الدهون الاخرى في المصل وكذلك بقياس تاثيره على مناطق الصدفية وشدة المؤشرPASI score. اجريت مقارنة بين السايتوكينات الدهنية و(ox - LDL) المؤكسد مع مؤشر كتلة الجسم (BMI) ومنطقة الصدفية وشدة المؤشر (PASI) وقيمهم التخمينية في وباء الصدفية. اخيرا لتاسيس دور المعطيات المذكورة اعلاه في عملية نشؤ المرض( داء الصدفية). المواد وطرق البحث : اشتملت الدراسة على ست وثمانون شخص (86) من الحالات المطابقة في الجنس والعمر المطابقة للدراسة الحالية (43 مصابين بالصدفية و43 اشخاص اصحاء) اما الحالات المصاحبة بارتفاع ضغط الدم او داء السكري قد تم استبعادها من هذه الدراسة. متوسط العمر كان (41±14.37) للمرضى، بينما كان (35±18.66) لمجموعة الضبط. تم اجراء هذه الدراسة في استشارية الجلدية في مستشفى مرجان التعليمي في مدينة الحلة، وتم اجراء الاختبارات في مختبرات المستشفى ومختبر قسم الجهاز الهضمي خلال الفترة بين (حزيران 2013) الى (اب 2014). تم سحب عينات المصل الماخوذ من الدم لاستخدامه من اجل ايجاد مستوى واجراء اختبارات لقياس التغيرات الحاصلة في مستويات الفيزفاتنvisfatin ، السايتوكينات الدهنية ذات اوزان جزيئية كبيرةHMW - adiponectin ، وبروتينات رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الواطئ الكثافة المؤكسدox - LDL ومستوى المحتوى الدهني العام في المصل. تم قياس هذه السايتوكيتات في جميع الحالات بواسطة طريقة الفحص المناعي ELISA. تم تقييم نشاط داء الصدفية وكفاءة الميثوتريكسيت بواسطة مؤشر شدة المرض (PASI) وتاثير الميثوتريكسيت على مستويات السايتوكينات الدهنية المذكورة اعلاه والبروتينات الدهنيه المؤكسدة ذات الكثافة الواطئةox - LDL في المصل. النتائج : اظهرت نتائج هذه الدراسة زيادة واضحة (p<0.05) في الفيزفاتنvisfatin ، الاديبونكتن ذات اوزان جزيئيه كبيرةHMW - adiponectin، ونسبة تركيز البروتين الدهني المؤكسد الواطئ الكثافة (ox - LDL) ، ونقصان واضح (p<0.05) في نسبة تركيز البروتين الريتونولي الرباعي الارتباط (RBP - 4) في مصل مرضى الصدفية عندما تقارن مع مجموعة الضبط. اظهرت الدراسة ارتباطا كبيرا بين (ox - LDL) والفيزفاتن visfatin، وبين الاديبونكتين ذو اوزان جزيئيه كبيرهHMW - adiponectin وكذلك البروتين الرباعي الارتباط الريتونوليRBP - 4. فيما اظهرت نتائج الدراسة انه ليس هنالك علاقة بين الفيزفاتن والاديبونكتن ذو اوزان جزيئية كبيرة والبروتين الدهني الواطئ الكثافة المؤكسد ومؤشر كتلة الجسم (BMI) بينما بينت الدراسة علاقة سلبية واضحة بين البروتين الرباعي الارتباطRBP - 4 مع BMI. من ناحية اخرى اكدت النتائج ارتباطا كبيرا مباشرا بين الفيزفاتنvisfatin والاديبونكتن ( HMW) و(ox - LDL) ومؤشر الشدة (PASI) في هذه الدراسة، بينت نتائج الدراسة ايضا ارتباطا سلبيا واضحا بين (PASI) والبروتين الرباعي الارتباط RBP - 4. بالاضافة الى ذلك يوجد اختلاف واضح في معدلات مستوى الدهون في مصل مرضى الصدفية عند المقارنة مع مجموعة الضبط. اخيرا اوضحت الدراسة ، تاثير الميثوتركسيت على مستوى السايتوكينات و(ox - LDL) ومستوى دهون الدم من خلال الانخفاض الكبير في مستوياتها بعد المعالجة وبواسطة متوسط الاختلاف الكبير في قيم (PASI) قبل وبعد المعالجة لمرض الصدفية. الاستنتاجات : تشير نتائج هذه الدراسة الى ان بعض السايتوكينات الدهنية في مصل الدم ومنها انزيم الفيزفاتنvisfatin ، الاديبونكتين ذات اوزان جزيئية كبيرة HMW - adiponectin، والبروتين ذو رابطة ريتونولية رباعية (RBP - 4) مع البروتين الشحمي الواطئ الكثافة المؤكسدox - LDL)) في مرضى داء الصدفية يمكن ان تكون مؤشرات جيدة للتنبؤ بشدة مرض الصدفية وكيفية متابعة العلاج. عند اجراء مقارنة في مرضى داء الصدفية بين السايتوكينات الدهنيه والبروتين الشحمي الواطئ الكثافة المؤكسدox - LDL مع مناطق الاصابة بالصدفية ومؤشر الشدة (PASI) اظهرت الدراسة ارتباط ايجابي ايجابيا مع فيزفاتن، الاديبونكتن ذات اوزان جزيئية كبيرة و(LDL)ox) المؤكسد،، بينما يرتبط سلبيا مع بروتين الرباعي الارتباط الريتنولي RBP - 4بينما اظهرت الدراسة عدم وجود ارتباط بين هذه السايتوكينات ومؤشر كتلة الجسم (BMI).اظهرت الدراسة علاقة قوية بين شدة المرض ومستوى دهون الدم المختلفة في مرضى الصدفية من خلال قياس مؤشر الشدةPASI)) قبل وبعد اخذ علاج MTXكما بينت نتائج الدراسة تاثيرا واضحا للميثوتريكسيت الفموي على مستوى دهون الدم و(ox - LDL) المؤكسد في مرضى الصدفية، بالاضافة الى تحسن سريع في حجم الصدفية، مما يشير الى الدور المناعي لهذا العلاج في داء الصدفية. | Psoriasis is a common inflammatory autoimmune skin disease that affects 2 - 4%of the world population. T - cells infiltration, interleukins and cytokines including adipocytokines like visfatin, high molecular weight - adiponectin(HMW - adiponectin) and retinol binding protein - 4(RBP - 4), together with oxidized low density lipoprotein(ox - LDL) have been implicated in psoriasis pathogenesis. Many epidemiological evidence speculate that patients with psoriasis may be more obese compared with the general population. Although the definite mechanism underlying the correlation between obesity and psoriasis is uncertain, several studies had hypothesized that, the secretion of pro - inflammatory cytokines by adipose tissue may deteriorate psoriasis. Objectives : The aim of the present study is : To investigate the changes of serum visfatin level, HMW - adiponectin, RBP - 4, and oxidized - LDL in psoriatic patients before and after treatment with oral methotrexate therapy in comparison to their levels in control subjects to evaluate the impact of this drug on their serum levels and on psoriatic lesions by measuring psoriasis area and severity index(PASI). To ascertain the association between these adipocytokines and oxidized - LDL with body mass index(BMI) and psoriasis area and severity index(PASI) and their predictive value in plaque psoriasis . Finally, to establish the role of above mentioned parameters in the pathogenesis of psoriasis. Subjects and Methods : A total of 86 subjects (43 with Psoriasis and 43 healthy controls) were enrolled in this study. Their mean age was (41± 14.37) for patients, and (35.81± 18.66) for the control group. The study conducted in dermatology outpatient clinic in Merjan Teaching Hospital in Hilla City, and investigations were done in laboratory units of the hospital through the period from June 2013 to September 2014. The sera obtained from blood were used to determine the level of serum visfatin, HMW - adiponectin, RBP - 4, oxidized - LDL by enzyme - linked immunosorbent assay(ELISA), while lipid profile levels were estimated by spectrophotometric kits. Assessment of psoriasis disease activity and methotrexate efficacy were done by psoriasis area and severity index (PASI)score and methotrexate effects on serum levels of the above mentioned adipokines and oxidized low density lipoprotein.Exclusion criteria include : any person with hypertension, diabetes mellitus, pregnancy, smoking, alcoholism, and other medical diseases even other types of psoriasis disease.Results : Results of this study showed significant increase (p˂0.05) of serum visfatin, HMW - adiponectin, and oxidized - LDL concentrations, and significant decreases (p˂0.05) of retinol binding protein - 4(RBP - 4) concentration in sera of plaque psoriasis when compared with control group. There was a significant correlation between oxidized - LDL and visfatin, HMW - adiponectin and retinol binding protein - 4(r = 0.81, 0.68, ̵ 0.77)(p value˂0.001) respectively . Regarding the anthropometric parameters, this study shows no correlation between visfatin, HMW - adiponectin, RBP - 4, oxidized - LDL and body mass index(BMI)(r= 0.121, 0.043, - 0.170, - 0.049)respectively(p˃0.05). The findings of the present study confirm a significant association between visfatin, HMW - adiponectin, RBP - 4 and oxidized - LDL and PASI score(r= 0.77, 0.64, ̵ 0.77, 0.72)(p˂0.001) respectively. Additionally, there was a significant mean difference in lipid profile in serum of psoriatic patients when compared to control subjects(p value˂0.001). Finally, the impact of systemic methotrexate on above adipocytokines and oxidized - LDL was determined by significant reduction of their levels after treatment and by a significant mean difference in PASI score before and after treatment in psoriatic lesions size. Conclusions : Results obtained by this study indicate that some adipocytokines including serum visfatin, HMW - adiponectin and RBP - 4 levels, in addition to oxidized - LDL measured in patients with plaque psoriasis were closely associated with disease severity and could be used for prediction and treatment follow up.In patients with plaque psoriasis the PASI score is positively associated with serum visfatin, HMW - adiponectin and oxidized - LDL, while negatively correlated with serum retinol binding protein - 4 level, while the study results revealed no significant correlation between BMI and study parameters. This results may indicate that, these parameters could be independently regulated through the path of psoriasis process.The study findings confirm the association between psoriasis and abnormal lipid profile and also the study showed a significant correlation between lipid profile and PASI score(p˂0.05).The study results showed the good effect of oral methotrexate upon the serum adipocytokines and oxidized - LDL - c levels in psoriatic patients, in addition to better remission of psoriatic lesions, which indicate the immune modulatory role of this drug in psoriasis

دراسة جزيئية وبكتريولوجية لبكتريا Porphyromonas gingivalis المعزوله من المرضى المصابين بامراض اللثه == Molecular and Bacteriological Study of Porphyromonas gingivalis Isolated from Patients with Periodontitis

اسم المؤلف: فاطمة مالك عبود
اسم المشرف: محمد صبري عبد الرزاق السعيد | الهام عباس بنيان
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تم جمع 103عينة من الصفيحة الجرثومية العميقة (subgingival dental plaque) من المرضى الذين يعانون من التهاب اللثة المزمن والمتقدم (72, 31) على التوالي لمراجي العيادة الاستشارية لامراض اللثة في كلية طب الاسنان/ جامعة بابل والمركز التخصصي لطب الاسنان في مدينة الحلة. للفترة من كانون الاول 2013 الى حزيران 2014. تم تشخيص وعزل بكتريا P. gingivalis بطرق مختلفة منها استخدام الطرق البكتيرية التقليدية واظهرت النتائج ان 69.5%) 16) منها تم عزلها من التهاب اللثة المزمن وتم عزل 7 (30.5) من التهاب اللثة المتقدم باستخدام التنمية على الوسط الانتقائي الخاص لهذه البكتريا. وكذلك تم استخدام طريقة التشخيص الجزيئي باستعمال المعلم الوراثي المستند على دور جين 16s rRNA حيث اظهر ان12 عزلة من اصل 23 عزلة تم تشخيصها بشكل نهائي انها تعود لبكتريا P. gingivalis. منها حوالي 8 تعود الى التهاب اللثة المزمن و4 تعود الى التهاب اللثة المتقدم. بالاضافة الى ذلك, تم التحري عن قابلية هذه البكتريا للالتصاق(adherence ability) بالخلايا الطلائية المبطنة للفم حيث وجد ان جميع العزلات لها القابلية على الالتصاق بهذه الخلايا وتم ايضا اختبار قابليتها على انتاج انزيم (Gingpain) ووجد ان جميع العزلات منتجة الى هذا الانزيم.في هذه الدراسة تم التحري المظهري عن عملية تكوين الاغشية الحيوية(Biofilm formation) للعزلات المدروسة باستخدام فحص tissue culture plate test (TCP) اذ كانت مكونة للاغشية الحيوية بنسبة 100% وكذلك تم فحص تاثير بعض المواد المستخدمة في طب الاسنان على انتاج biofilm ووجد ان اعلى تثبيط سجل للكلورهكسدين (غسول الفم) والشب (Alum)ويليه تاثير Clove مقارنة باقل تاثير لمادة السعد ((Cyprus rotundus. وكذلك تم الكشف عن حساسية العزلات لبعض المضادات الحيوية ووجد بان كل العزلات كانت حساسة الى Co - amoxiclave وعلى العكس اغلب العزلات تظهر مقاومة عالية لل Amoxicillin بينما سجلت مقاومة قليلة جدا لل Ampicillinو Metronidazole(16.3%, 20.3%, ) على التوالي. واخيرا تم فحص تاثير بعض المواد المستخدمة في طب الاسنان على تثبيط نمو هذه البكتريا وتوضح النتائج ان اعلى تاثير سجل للكلورهكسدين (غسول الفم) والشب (Alum)ويليه تاثير القرنفل(Clove) mm) 25,30,40) على التوالي مقارنة باقل تاثير لمادة السعدC. rotundus (10mm) .في هذه الدراسة ايضا تم التحري عن التغاير الجيني ((TLR - 4 Polymorphisms باستخدام تقنية البلمرة والقطع بالانزيم ((PCR - RFLP. وتبين من النتائج وجود ثلاث انماط جينية في موقع طفرة Thr399Ile : CC وCT وTT ، اذ بلغت نسبة انتشارها ((12.5%,10%,77.5% في مجموعة المرضى على التوالي بينما هناك فقط نمطين في مجموعة السيطرة TT,CCوبنسبة انتشار(36.6% ,63.4%) على التوالي ، فيما بلغت نسبة انتشار الاليلC السائد في المرضى 82.5 % بالمقارنة للسيطرة 63.3% مع وجود اختلاف معنوي. وفي المقابل سجل وجود ثلاث انماط جينية في موقع طفرة Asp299Gly : AA وAG وGG وبنسبة انتشار % 67.5 و12.5% و20% في مجموعة المرضى على التوالي بينما هناك فقط نمطين في مجموعة السيطرة GG, AA وبنسبة انتشار34.4%) 66.6%,) على التوالي. حيث سجلت نسبة انتشار الاليل A السائد (73.75%) في مجموعة المرضى بالمقارنة بمجموعة السيطرة (66.6%) وبفرق معنوي. | In this study, 103 clinical samples were collected from subgingival dental plaque of patients with chronic (CP) and aggressive periodontitis (AP) (72 and 31) respectively, and (n=30) a healthy group admitted to teaching Hospital in college of Dentistry / Babylon University and specialized center of Dentistry in Hilla city, From February 2013 to June 2014). Out of which (n=40) with a chronic periodontitis group from them blood sample were collected for detection of gene polymorphisms in Toll - like receptor - 4 (TLR - 4). These dental plaque samples were subjected to different methods for identification of P. gingivalis mainly traditional bacteriological method. It was found that 23 P. gingivalis isolates were recovered by using selective media where 19 isolates (26.3%) obtained from (CP) and 4 isolates (12.5%) from (AP). Furthermore, molecular detection method was applied by using 16s rRNA gene as a genetic marker for confirmation of detection of P. gingivalis isolates, 12 isolates of P.gingivalis out of 23were detected by molecular method focusing on the role of 16s rRNA gene of P. gingivalis. 8 isolates were isolated from CP, and 4 isolates were isolated from AP subgingival plaques. In addition, these 12 isolates were investigated to detect Adherence of P.gingivalis to oral epithelial cells. The result showed that all isolates have ability to adhere to oral epithelial cells. The study also detected the ability of this bacteria for production of Gingipain, it was found that all isolates were positive for this enzyme at a rate 100%.Furthermore, biofilm formation was tested in the semi quantitative microtiter plate test. The results revealed that all isolates were biofilm former, high and moderate biofilm formation mode were accounted for (50%) while there are no isolates that express non biofilm formation.Regarding to effect of Chlorhexidine (CHX) gluconate (0.12%) on biofilm formation, the results of this study demonstrated (CHX) effectively and reduced the viability of biofilm - forming bacteria from moderate to 25%, weak to 75% and no strong. The study was also evaluating the effect of some plant extracts on biofilm formation. The results showed the highest effect for Alum potassium phosphate followed by Clove, in contrast to lowest effect of C. rotundus. And after screening the effect of Flagyl solution on production of bacterial pigment. The results showed that the metronidazole produced the highest inhibition activity range (30 - 10mm). Furthermore, the susceptibility of isolates to a variety of antibiotics had been investigated and it had been found that all isolates were sensitive to co - amoxiclave and, in contrast, the isolates showed high resistance to other type of beta lactam such as amoxicillin (54.3%). In addition, the result showed that the resistance of p. gingivalis was very low for ampicillin and metronidazole (16.3% and 20.3%), respectively.The screening of antimicrobial activity of (CHX) gluconate (0.12%) was carried out and the results showed that it produced the highest inhibition activity whether against p. gingivalis with inhibition zone range 40 - 15mm. Finally in vitro antibacterial activity of Alum, Clove and Cyperus rotundus plant extracts was studied and the results revealed that all tested isolates were inhibited by aqueous extracts at 50% concentration. The maximum inhibition zone was observed in Alum and Clove extracts respectively (30mm, 25mm), compared to the minimum inhibition by Cyperus rotundus (10mm). The antibacterial actions of 20% concentration of plant extract gave lower inhibition zone than 50% concentration of that extract which represented by (19mm, 16mm, and 10 mm) of the previous three mentioned extracts. This study also aimed to investigate the association of Thr399Ile and Asp299Gly single nucleotide polymorphisms (SNPs) in the (TLR - 4) with the incidence of (CP).The results revealed at the site of SNPThr399Ile, there were three genotypes for this SNP among CP patients; CC, CT and TT with frequency of 77.5%, 10% and 12.5 % respectively, whereas, there were only two genotypes among control group; CC and TT with frequency of 63.4% and 36.6% respectively with no significance differences between patients and control. However, the frequency of allele C (dominant) was higher among CP group (82.5%) than control (63.3) with significant difference. The site of SNP of Asp299Gly also showed three genotypes among CP patients; AA, AG and GG with frequency of 67.5%, 12.5% and 20% respectively, whereas, there were only two genotypes among control group; AA and GG respectively with frequency of 66.6 and 34.4% respectively with no significance differences between patients and control. However, the frequency of allele A (dominant) was higher among CP group 73.75 % than control 66.6%.

دور السايتوكينات المعزولة من انسجة المشيمة المغذية للجنين لدى النساء مع الاجهاض التلقائي == The role of cytokines isolated from feto - placental tissues in women with spontaneous miscarriage

اسم المؤلف: ميسلون عبد الحميد ناصر
الموضوع العام: الطب
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: شملت الدراسة الحالية (172( امراة، تتراوح اعمارهم بين 16 - 41 عاما، من المرضى الذين قدموا الى مستشفى الولادة والاطفال في البصرة بسبب الاسقاط التلقائي غير الكامل في الشهور الثلاثة الاولى من الحمل ، بالاضافة الى النساء مع الحمل الطبيعي في وقت الولادة خلال الفترة من شباط 2012 الى ايار 2014 وتم تقسيمهن الى 3 مجموعات :  المجموعة - ا : وشملت )65) من النساء مع الاجهاض التلقائي المتكرر (RSM) خلال الاشهر الثلاثة الاولى، مع متوسط العمر (25.2 ± 7.28) سنة. المجموعة - ب : وشملت (36) من النساء مع الاسقاط التلقائي غير الكامل في الاشهر الثلاثة الاولى من الحمل ولها على الاقل ثلاثة مرات حمل طبيعي سابق (الاجهاض التلقائي غير المتكرر ( non - RSM بمتوسط من العمر (26.61 ± 6.97) سنة. المجموعة - ج : السيطرة (الحمل الطبيعي) : 71 امراة بمتوسط من العمر (26.17 ± 7.01) سنة. وتم الحصول على الدم الوريدي من المجاميع قيد الدراسة من اجل البحث عن المضاد المناعي م وج الخاص بمقوس قوندي , IgM ) IgG (. والتشخيص المصلي للاصابة بمقوسات قوندي يستخدم على نطاق واسع لفحص النساء الحوامل والمجهضات. كان معدل حدوث داء المقوسات (38.4٪) التي وجدت في 101 مجموعات الاجهاض التلقائي بواسطة فحص (ELISA). اظهرت عينتين (3.08٪) نتائج ايجابية المضاد المناعي (م). بينما كانت 24 (36.92٪) عينة ايجابية للمضاد المناعي (ج) لمقوس قوندي في مجموعات المرضى , بينما لم تسجل اصابة في 71 امراة من مجموعة السيطرة . غالبية النساء المصابة (50٪) في سن (30 - 39) سنة في حالة المضاد المناعي (ج)، في حين ان (10.26٪) في سن (20 - 29) سنة في حالة من المضاد المناعي (م).والنتائج كشفت عن ان اعلى نسبة للاجهاض التلقائي لوحظت في نساء الارياف . وفي الوقت نفسه، تم جمع نسيج الارومة المغذية للجنين خلال عملية الكشط والدم الوريدي للكشف وتقييم السيتوكينات المضادة للالتهابات (IL - 4، IL - 6، IL - 10) والسيتوكينات الموالية للالتهابات (IL - 2،عامل نخر الورم - الفا TNF - α، - γ IFN) وقياس تركيزها باستخدام فحص انزيم مرتبط البلمرة المناعي ، تبين تركيز عال (49.97 ± 8.82، 180.7 ± 15.03، 94.34 ± 7.68) للسيتوكينات في الارومة المغذية للجنين (IL - 4، IL - 6، IL - 10 على التوالي) في حالة مجموعة السيطرة بالمقارنة مع مجموعات المرضى وتم الكشف عن نتيجة عكسية في مصل الدم ، في حين ان التراكيز عالية من السيتوكينات الموالية للالتهابات (IL - 2، TNF - α، IFN - γ) كانت (134.17 ± 24.37، 432.16 ± 51.7، 100.46 ± 9.94 على التوالي) في حالة مجموعة الاجهاض التلقائي المتكررRSM)) بدلا من مجموعة السيطرة ومجموعة الاسقاط التلقائي غير المتكرر RSM - non مع وجود اختلافات معنوية كبير.p<0.001 وقد تبين في الدراسة الحالية وجود علاقة واضحة بين مناعة الامهات نوع TH2 والحمل الطبيعي، في حين يرتبط رد فعل جهاز المناعة نوع TH1 بفقدان الحمل. والتوازن الدقيق بين السيتوكينات الناتجة عن TH1 / TH2 ممكن ان يمنع خمج مقوسات قوندي . الذي يرتبط مع الانتاج الكبير من TH1 للسيتوكينات الموالية للالتهابات.وعلاوة على ذلك نتائج حساب مستويات CD44، اظهر فرقا معنويا عاليا p<0.001 في حال مقارنة بين مجموعة السيطرة والمجموعتين من النساء المجهضات . وهكذا تم تسجيل ارتباط CD44 مع فعالية خلايا TH2 ؛ لذا فمن المرجح انه قد يكون هناك وجود علاقة بين الحمل الطبيعي وكثافة CD44 على سطح الخلية

تشخيص فطري Trichoderma viride وTrichoderma harzianum بتقنية PCR واستخدامهما في تحسين القيمة الغذائية لمتبن واداء الحملان العرابية == Identification ofTrichodermaviride andTrichodermaharzianum by using Molecular Technique and Used to ImproveFeeding Value of Straw and Performance of Arabi Lambs

اسم المؤلف: ميثم خلف علي الغالبي
اسم المشرف: عماد فلاح حسن | طالب احمد جايد
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The objectives of current study were to identify Trichoderma viridi and Trichoderma harzianum by genetics methods and used them singly or both (3% for each treatment) in improvement of barley straw and animal performance. A total of 20Arabi lambs aged about 3 - 4months with an average weight of 22.725±1.43 kg distributed randomly and equally to four nutritional treatments. Lambs fed individually as 2% of body weight, while straw was given ad libtium. The results obtained can be summarized as follows : 1 - The study was able to identify TV and TH using three different PCR techniques, which are PCR - RAPD, PCR - ITS and PCR - IGS.TV and TH showed three and two bands respectively when using PCR - RAPD at nearly 600bp.While both fungi showed one band by using PCR - ITS and PCR - IGS and at nearly 600 and 2,400bp respectively.2 - Using both fungi (treatment 4) resulted in a significant improvement in the chemical composition of the straw through the high content of crude protein and low crude fiber, ADF and NDF compared to untreated straw.3 - There were improvements indigestibilityofcrude protein, fiber, cellulose, hemicellulose, ADFand NDF when the both fungi treatment was used. As well as total digestible nutrients of the fungus treated groups in comparison with untreated straw (control group).4 - Feed intake of treated straw groups increased significantly compared to untreated straw groupwiththe superiority of the group that was treated with both fungi (639.91 - 826.30 gm/head/day for control and both fungi group respectively). As well as there wasa significant improvement in feed conversion efficiency of a group treated with both fungi in comparison of other groups (6.38 - 5.52 kg feed/kg growth rate respectively).5 - Rumen pH, ammonia and total volatile fatty acid of lambs fed treated straw (6.22 - 6.89, 10.00 - 18.69; 11.35 - 13.61 for control115group and both fungi group respectively) were significantly higher than those of untreated group.6 - There were significant improvements in lambs’bodyweigh tsand their absolute and relative gains when fed treated straw (37.82 kg, 14.97kg and 65.67% respectively) especially the group fed both fungi in comparison with untreated group (32.79 kg, 10.03kg and 43.89%respectively).7 - Globulin, AST and ALT was not significantly influenced bytreated straw. On the contrary, there were a significant increase inhemoglobin, glucose, total protein, albumin and albumin/globulin ratio of the treated groupcompared with the untreated straw. While a significant decrease in urea and cholesterollevelin theblood serumof treated groups in comparison with untreated group.8 - Economic evaluation ofadding fungitostrawshowed improvement inthe relativeeconomic efficiencyby26.2%, 37.0% and 59.5% for adding TVorTH or bothtogetherrespectively. Adding fungi decreasedthe cost of feed from 46.65Dinars/ kg increase in body weight gains incase of untreated straw to 40.15dinars/ kg when adding both fungi.

تاثير حقن بيض التفقيس ببعض المغذيات والتغذية المبكرة في بعض الصفات الانتاجية والفسيولوجية لفروج اللحم == The effect of in ovo - injection of Hatching Eggs with Some Nutrients and early Feeding on Productive Performance and Some Physiological Traits of Broiler Chicks

اسم المؤلف: هدى فالح سعد الساعدي
اسم المشرف: رياض كاظم موسى | طارق فرج شوكت
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: This study was undertaken at the poultry field of Animal ResourceDepartment \college of Agriculture \University of Basra in Jan.,28th toMar.,3th,2013 and in Oct.,28th to December,23th 2013 for the first and secondexperimentrespectively . The objective of this study was to evaluate the effectof hatching eggs injection with the some nutritious (Folic acid vitamins,Pyridoxine, Threonine amino acid) for the first experiment and choosing besttreatment from first experiment and injection with Arginine amino acid ,folicacid as well as the effect of feeding time on the performance and somephysiological traits of broiler chicks .In the first experiment ,825 hatching eggsof Ross 308 broiler were received from the hatchery .At 14th day of incubationstage ,eggs were tested and unfertile eggs were discarded ,the remainder eggswere divided into seven treatments and injected with 0.1 ml of solution in airsac position of the egg as follows : 1. First treatment : injection 100 eggs with 0.1ml of threonine solution (30μgconcentration per an egg).2. Second treatment : injection 100 eggs with 0.1 ml of b6 vitamin solution(100μg concentration per an egg)3. Thirdtreatment : injection 100 eggs with 0.1 ml of mix from L - threonine aminoacid plus b6 vitamin (30μgand 100μg) per an egg.4. Fourthtreatment : injection 100 eggs with 0.1 ml of Folic acid vitamin solution(250g) per of an egg.5. Fifthtreatment : injection 100 eggs with 0.1 ml of mix solution from Lthreonineamino acid plus Folic acid vitamin (30μg and 250 g) per an egg.6. Sixthtreatment : positiveControl : injection100 eggs with 0.1 mlSterile distilledwaterper egg.7. Seventhtreatment : negativeControl : 155 eggs were left withoutinjection.Hatching chicks of each treatment were distributed in to three replicate (15 Chicksfor each replicate) .Birds were reared up to five weeks of age.The results of first experiment painted out to : 1. There was significantly (P<0.05) proceeding for the treatment of egg injectionwith some nutrients on hatching percentage ,hatching chick weight ,bodyBweight ,body weight gain and weekly and Cumulative feed conversion ratio.whereas, this treatment showed less embryonic mortality percentage incomparison with other experimental treatments and There was significantly(P<0.05) increase in weekly and Cumulative feed intake of birds that werehatched from injected eggs with ( folic acid ,threonine amino acid) during thesecond ,fourth and fifth week were as ,the treatment of pyridoxine injectionshowed less mortality percentage compared to other experimental treatments .2. Treatment of folic acid and threonine and pyridoxine injection showedsignificant (P<0.05) increases in the dressingpercentage, weights of breast,thigh and edible viscera comparison with other treatments.3. There was significantly(P<0.05) increase in red blood cells number,hemoglobinconcentration package cells volume of broilers that were hatchedfrom egg injection with (folic acid plus threonine) at 3 and 5 weeks of agecompared to other treatment While , Treatment of Pyridoxine eggs injectionshowed highest mean of white blood cells at 3 weeks of age, whereas,treatment of Pyridoxine and folic acid eggs injection proceeding otherexperimental treatment in highest mean of white blood cells at 5 weeks of age .4. There was significantly(P<0.05) increase in total protein concentration,glucose ,Globulins, and less uric acid concentration and cholesterol in chicksblood serum of folic acid and threonine egg injection treatment at 3 and 5weeks of age in comparison with other treatment. Whereas, there were nosignificant effect of egg injection on triglycerides concentration and on T3 andT4 hormone in broiler blood serum.5. There was no significantly effect of egg injection with nutritive on low densityfatty proteins concentration whereas,the negativecontrol treatment showedhighest concentration of low and high proteins at 3 and 5 weeks of agecompared to other experimental treatments.6. T4 hormone concentration in hatchingbroiler blood serum of threonineinjection treatment increased significant (P<0.05) at 3 weeks of age ,whereas,T4 hormones concentration was higher respecting treatment of folic acid andthreonine injection at 5 weeks of age in comparison with other treatment.7. There was significantly (P<0.05) increase in phosphorus ,calcium and ferricconcentrations in blood serum of the hatchingbroilers of folic acid andthreonine egg injection treatment at 3 and 5 weeks of age in comparison withother treatments.C8. There was significantly (P<0.05) increase in the villus lengths cryptsdepth ofjejunum of the boilers Caracasrespecting Folicacid and Threonine injectiontreatment in comparison with other treatment.According to the firstexperiment results and choosing the best treatment of egginjection with nutritive, the second experiment was carried out .the treatmentof this experiment whereas follows : 1. Firsttreatment : hatching eggs injection with (30μg of threonine, 250g of folicacid) per an egg.2. Secondtreatment : hatching eggs injection with (5 μg of arginine) per an egg.3. Thirdtreatment : hatching eggs injection with (5 μg of arginine plus 250 goffolic acid) per an egg.4. Fourth treatment : the control treatment (withoutinjection).Hatching chicks of each treatment were divided in to groups each of threereplicates ( 12 Chicks for each replicates ) .firstgroups chicks were fed at thehatchery ,whereas,second groupschicks were fed after 24 hours at field. Chickswere reared from 1 day after hatching up to 5 weeks of age .the results of thesecond experiment pointed out to : 1. Treatments of injection with arginine and Folic acid and threonine plus folicacid proceeding significantly (P<0.05) in hatching percentage andhatchingchicksweight, whereas, This treatment showed less percentage of lateembryonic mortality in comparison with other treatments. Also, Broiler birdsof Arginine and Folic acid injection treatment showed significantly (P˂0.05)increase in body weight, weekly body again, weekly and Cumulativefeedintake,weekly and Cumulative feed conversion ratio and dressing percentage.Whereas, treatment with arginine and folic acid injection showed less totalmortality mean compared to other treatments. Also, there was significantly(P˂0.05) effect of feeding time on some productive traits. Treatments offeeding at the hatchery showed significant increase in body weight, bodyweight gain, feed intake, better feed conversion ratio and dressing percentagein comparison with treatments of feeding at field after 24 hours.2 - There was a significantly (P˂0.05) effect of interaction between eggs injectionwith nutritious and feeding time on body weight, body weight gain and feedconversion ratio. Treatment of folic acid arginine (hatchery feeding) showedDhighest body weight, body weight gain, better feed conversion ratio incomparison with the control treatment (Field Feeding after 24 hours).Treatment of arginine injection (HatcheryFeeding) was highest dressingpercentage, whereas there was no significant effect of interaction on feedintake and total mortality.3 - Treatment of threonine and folic acid injection showed a significantly (P˂0.05)increase in bird’s body fullness, gizzard weight and Fabricia gland. Whereas,treatment of arginine and folic acid injection showed a significantly (P˂0.05)increase in breast fullness liver and heart weight in comparison with othertreatments.4 - Feeding time affected significantly (P˂0.05) on some carcasses traits. Carcassesof feeding at hatchery treatment were better significantly in body fullness,breastfullness, and heart, liver, gizzard, and Fabricia gland and pancreasweights. Whereas, carcasses of feeding at field treatment were of high pad fatweight. There was no significant effect of interaction between injection andfeeding time on carcasses traits.5 - There was a significant (P˂0.05) increase in red and white blood cells numbers 3days and 5 weeks of age and in hemoglobin concentration at 5 weeks of agerespecting the treatment of arginine and folic acid in comparison with othertreatments. Control treatment exceeded in Heterophil cells\ lymphocyte cellspercentage at 3 and 5 weeks of age compared to treatments.6 - There was a significantly (P˂0.05) effect of feeding time on some cellular bloodcharacteristics. Treatment of the field feeding exceeded in red blood cellsnumbers, hemoglobin concentration and Heterophil cells\lymphocyte cellspercentage at 3 days and 5 weeks of age. Whereas, treatment of the hatcheryfeeding exceeded in white blood cells numbers compared to treatment of thefield feeding after 24 hours.There was no significantly (P˂0.05) effect ofinteraction between injection and feeding time on the cellular bloodcharacteristics at 3days of age, whereas this interaction affected significantly(P˂0.05) on the white blood numbers and Heterophil cells\lymphocyte cellspercentage at 5weeks of age.E7 - Treatment of arginine and folic acid injection exceeded significantly (P˂0.05)in serum total protein concentration at 3 days and in total proteinconcentration, uric acid and GPT, GOT enzymes in blood plasma at 5 weeksof age. Treatment of threonine and folic acid in comparison with othertreatments.8 - Feeding time affected significantly (P˂0.05) on biochemical bloodcharacteristics. Treatment of the hatchery feeding exceeded in glucoseconcentration and total protein at 3 days of age and in total protein concentration and uric acid at 5 weeks of age. Treatment of the field feeding exceeded in blood serum cholesterol concentration in comparison with thetreatment of hatchery feeding. There was no significant (P˂0.05) effect ofinteraction between injection and feeding time on the biochemical bloodcharacteristics at 5 weeks of age. 9 - There was a significant (P˂0.05) increase in villus Length and crypts Depth of the intestinal jejunum of comparison with of threonine and folic acid injection treatment in comparison with other treatments. Treatment of the hatchery feeding exceeded (P˂0.05) respecting those traits compared to the field eeding treatment. There was a significantly (P˂0.05) effect of interaction (TheHatchery Feeding) and feeding time villus, length and crypts Depths werehighest at 1 and 5 weeks of age.

تاثير هيومات البوتاسيوم والكبريت في صفات التربة والنمو والحاصل وخزن الطماطة المزروعة تحت الانفاق البالستيكية في المنطقة الصحراوية == EFFECT OF POTASSIUM HUMATE AND SULPHUR ON CHARACTERISTICS OF SOIL , GROWTH, YIELD AND STORABILITY OF TOMATO (Lycopirscon esculentum MILL.) CUITURED UNDER PLASTIC IN DESERT REGION OUTHERN IRAQ

اسم المؤلف: ميسون موسى كاظم محمد الساعدي
اسم المشرف: عبد الله عبد العزيز عبد الله
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: النبات - البستنة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The present experiment was carried out during two winter grawing seasons (2013 - 2014 and 2014 - 2015) in one of the dry land farms,khor AL - Zubiar Basrah with the objective of studying.The response of the Hybrid tomato (Lycoperiscon esculentum mill.) CV.Hitouf grown under plast poly ethyien to varins fertilizer treatment of organic fertilizer pow humus and inorganic fertilizer Agriculture sulphur .to study of the effect of the fertilizer treatment was on soil physical characteristics,vegetative growth characteristics,flowering and leaf chemical constituents wells quantitative and and quantitative yield ,and storability of behavior of the fruit at room temperature or cold storage .The experiment included nine treatments in afactorial mannes which were the combination of three levels of potassium humate (0 ,255 ,510 kg K.haˉˡ) and three levels of Agricultural sulfur (0 ,205 ,510 kg S. .haˉˡ) the experiment was performeal as factorial with completely randomized block design (CRBD)with three replicater Mean treatment value ware compared using least significant L.S.D at 0.05 level.The results may be sumuarized as follows : 1 - phsical and chemical soil characteristics .The addition of potassium humate at 255 and 510 kg K.hˉˡ caused significant increase in soil water content as compared with control treatment during the second growing season ,wheres the level of 255 kg k.ˉˡ caused asignificant decrease in soil pH as compared with control treatment as well as the level 510 kg K.haˉˡ humate during the first growing season caused asignificant decrease in soil pH,and Ec soil electrical conductivity for both growing seasons,and asignificant in lreane in soil moisture contant the yield growing season .For sulphur treatment ,it was fourd that the addition of Agricultural sulphur at 205 and410 kg S.haˉˡ caused asignificant decrease in soil pH for both growing seasons and a significant increase in soil moisture contant for both seasons as compared with control treatment.However ,sulphur at 500However,sulphur at 410 kg S.haˉˡ caused asignificant decrease in soil Ec during the secand growing.The time period caused asignificant decrease in soil pH and Ec soil during both seasons wheres soil moisture contant increased significant as the time period increased ,2 - Vegetative growth characteristics The addition of K humate at 255 and 510 kg K.haˉˡ caused a significant increase in plant length and number of side shoots during both growing seasons ,as compared with control treatment . Those two levels of K humate also caused asignificant increase in stem diameter ,total leaf number and both fresh and dry weight of the plant during the secand of growing season.However ,K - humate at 72 kg .haˉˡ caused asginificant increase in leaf area during both growing season ,where the increase in total leaf number and fresh weight of the plant was for the first growing season only.Sulphur at 205 and 410 kg S.haˉˡ caused a significant increase in plant length ,leaf area and plant dry weight during both growing season whereas the levels of Khumate had a significant effect with respect to plant from weight during the first growing season ,and total leaf number during the secand growing season.However ,the addition of Khumate at 410 kg S.haˉˡ induced asignificant increase in leaf number of side shoots during the first growing seaeon as compared with the level 205 kg S.haˉˡ and control treatment .The level 410kg S.haˉˡ caused asignificant increase in plant fresh weightduring the second growing season as compared with the level 205 kg S.haˉˡ .3 - flowering characteristics.The addition of K - humate of 255 and 510 kg K.haˉˡ caused a significant increase in number of inflorescens ,number of flowers per inflorescens during both growing season compared with control treatments .However ,both levels reducal the percentage of flower abortion during the first growing season .At 510 kg K.haˉˡ percentage flower decreased as compared with 36 kg K.haˉˡ and control treatment. Sulphur treatment at 205 and 410 kg S.haˉˡ reduced the percentage of flower aboration significant as compared with control treatment during the first growing season.At 410 kg S.haˉˡ ,sulphur ,that was a significant increase in number of inflorescane as compared with contror during the first growing season .cowever ,sulphur had no significant effect on number of flowers per inflorescence during both growing seasons .The interaction treatments had no significant effects ,with the exception of percentage flower aboration during the first growing season.K humate at 510 kg K.haˉˡ caused asignificant decrese in percentage flower abortion as compared with K humate at 255 kg K .haˉˡ during the secand growing season.The addition of sulphur at 205 and 410 kg S.haˉˡ reduced the percentage of flower abortion significantly as compared with control treatment during the first growing season.However, sulphur at 410 kg S.haˉˡ increased significantly the number of inflorescence as compared with control duringthe first growing season .Sulphur had no significant effect on flower number prr inflorescence for both seasons.4 - Leaf chemical constituents.The addition of K humate at both levels(255 and 510 kg K.haˉˡ ) caused a significant increase in total soluble carbohydrates,K+/ Na+ ratio and a significant decrease in chloride ion during both growing season.There was also a significant in total chlorophylls, proline, and percentage of N,P,K,S,Mg and Ca during the first growing season only.There was a significant increase the percentage of K+ and adecrease in Na+ ion during the second growing season .K humate at 510 kg K.haˉˡ caused asignificant increase in total chlorophylls and proline and percentage of S and Ca during second growing season.Plantfertilized with sulphur at 205 and 410 kg S.haˉˡ had asignificantly higher concentrations of total soluble carbohydrates ,total chlorophylls and percentage of N,P,S,Mg during both growing seasons as compared with control plants such treatments also caused a significant increase in percentage K+ ion,K+/Na+ ratio during the first growing season.However ,proline content,Ca++ ion was significantly increased, wheras chloride ion (CLˉ) was significantly decreased due to sulphur fertilization,during the second growing season .Sulphur at 410 kg S.hˉˡ caused a significant increase in percentage of Ca++ during the first growing season,and K+ ion during both growing season in comparison with 250 kg S.hˉˡand control treatment.5 - Quantitative yield.Plant fertilized with K humate at 255 and 510 kg S.haˉˡ had a significantly higher average fruit weight during both growing seasons ,fruit number and plant yield and total yield during the first growing season and early yield during the second growing season however supplying K humate at 510 kg K.haˉˡ had a significantly higher early yield during the first growing season and in fruit number ,plant yield and total yield during the second growing season.As for fertilization with agricultural sulphur, plant fertilized with 250 and 410 kg S.haˉˡ had a significant higher fruit weight as compared with control treatment during both season fruit number ,plant yield during the first growing season and early yield during the second growing season .Sulphur fertilizer at 410 kg S.haˉˡ had a significantly higher fruit number,plant yield and early and total yield during the second growing season compared with control treatment.There was a significant interaction between studied factors in certain characteristics and both growing seasons.6 - Qualitative yield.Tomato plants fertilized with K humate at 255kg K.haˉˡ had a significantly higher total titratable acidity percentage during both growing seasons ,vitamin C contant during the first growing season ,and percentage dry matter during the second growing season in comparion with control treatment.Plants fertilized with 510 kg S.haˉˡ had a significantly higher total soluble solids content during both growing season and in percentage dry matter during the first growing season and vitamin Ccontent during the second growing season.Plant fertilized with agricultural sulphur at both levels (205 and 410 kg S.haˉˡ )had asignificantly highr total titratable acidity during both season asecompared with control treatment, and percentage matter during the second growing season.Plants fertilized with sulphur at 410 kg S.haˉˡ had a significantly higher percentage dry matter and vitamin C content during second growing season.7 - Storage behavior.A - Storage at room temperature 25±1c .Storage at room temperature percentage fruit decay and loss in weight decreased significantly for fruit fertilized with K humate at levels of 36 and 510 kg K.haˉˡ ,and there was an increase in total soluble solids.Also,fruit decay percentage significantly decreased and total soluble solids increased in fruits of plants fertilized with sulphur at 205 and 410 kg S.haˉˡ during both growing seasons,and adecrease in weight loss during second growing season ,however,sulphur at 410kgS.haˉˡ caused a significant decrease in percentage season.Percentage fruit decay ,weight loss and total soluble solids increased ,with increasing storage period during both growing season .However vitamin C content decreased with increasing storage period .B - The cold storage 5±1 c.Both percentage fruit decay and percentage loss in weight decreased whereas total soluble content increased in fruit of plants fertilized with potassium humate at the levels of 255and 510 kg K.haˉˡ significantly as compared with fruit of control plants and for both growing seasons .Also,the percentage loss in weight for fruits of plants fertilized with agricultural sulphur at 205 and 410 kg K.haˉˡ ,decreased significantly for both growing seasons.Percentage fruit decay decreased significantly during the first growing season as well as an increase in total soluble solids during the first growing season.However sulphur at 410 kg S.haˉˡ caused asignificant decrease in fruit decay percentage during the second growing season and an increase in total soluble solids during the first growing season.Decay percentage,percent of weight loss, and total soluble solids and titratable acidity increased with increasing storage period .There was also adecrease in vitamin C contant with increasing the storage period for both growing seasons.The method of storage had a significant effect on studied characteristics,as cold storage caused a significant decrease in percentage fruit decay during both seasons and weight loss during the first growing season ,as compared with storage at room temperature.However,storage at room temperature caused asignificant increase in ascorbic acid content ,and total titratable acidity of the fruit as compared with cold storage and for both growing season .The total soluble content of the fruits increased for there stored at room temperature as compared to cold storage during the first growing season only.

استخلاص وتوصيف الكولاجين من وتر ارجل الجاموس وجلد سمك المزلق ودراسة بعض صفات الكولاجين المتحلل انزيميا == Extraction And Characterization of Collagen From Buffalo Tendon Legs and Fish Oriental Sole Skin and Study of Some Properties of Enzymatically Hydrolysed Collagen

اسم المؤلف: محمد زيارة اسكندر
اسم المشرف: ام البشر حميد جابر الموسوي
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الاغذية
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:

التشكل الوراثي لجيني اللاكتوفرين والبرولاكتين وعلاقته بانتاج الحليب ومكوناته واداء الحملان قبل الفطام في النعاج العرابية == Relationship between Lactoferrin and Prolactin Genes Polymorphism and Milk Yield and its Components and Pre - weaning Lambs Performance of Arabi Ewes

اسم المؤلف: علي جاسم عبد الرضا
اسم المشرف: اسعد يحيى عايد | سمير اسطيفان حنا
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الحيوان - التربية
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Polymorphism analysis of lactoferrin and prolactin genes and its relationship with milk yield and its components and birth, weaning weight and growth rate before weaning were studied. DNA was extracted from 77 blood samples of Arabi ewes (21 ewes from Animal Farm, College of Agriculture, University of Basrah and 56 ewes from Animal Farm, College of Agriculture, University of Al - Muthana). Lactoferrin genotypes were determined by using Hind III restriction enzyme, those of prolactin by Hae III enzyme at Genetic Engineering Laboratory/ College of Agriculture/ University of Basrah and College of Animal Resources/ Mullah Thani University/ Islamic Republic of Iran. Milk yield was measured once a week from 52 ewes belong to Animal Farm/ College of Agriculture/ University of Al - Muthana. Samples were chemically analyzed. Birth and weaning weight were recorded as well as growth rate before weaning during a period of 1/2/2013 to 31/12/2013. Results revealed the following : 1 - The possibility of amplifying both genes and get two alleles for each and three genotypes of the lactoferrin gene (AA and AB and BB) and two genotypes (AA and AB) of the prolactin gene.2 - Prolactin and lactoferrin A allele frequencies were 0.94 and 0.66 respectively. AA genotypes frequency was 0.88 and 0.52; AB 0.12 and 0.29 and BB 0.00 and 0.19 of prolactin and lactoferrin genes respectively. population was at equilibrium for prolactin gene, but was not in lactoferrin gene.3 - Heterozygosity levels were 11.69% and 28.57% for prolactin and lactoferrin genes respectively. inbreeding coefficients (Fis) were - 0.05 and 0.345 for both genes respectively. University of Basrah and Al - Muthana herds showed very high genetic correlation (0.999).4 - The overall means of total and daily milk yields and lactation length were 61.13±2.76 kg, 0.582±0.04 kg and 104.75±1.95 days respectively. Ewe age and lamb sex did not significantly influence the above traits. However, ewes lambed during February exceeded thoselambed in September by daily milk yield (0.661 vs 0.577 kg) and lactation period (110.00 vs 100.00 days).5 - The overall means of total fat, protein and SNF and their percentages were 2.65 Kg and 4.33%, 2.5kg and 4.11% and 11.98% respectively. Only SNF was significantly influenced by ewes age and lamb age.6 - The overall means of birth and weaning weights and pre - weaning daily growth rate were 4.48±0.39 kg, 21.21±0.89 kg and 161.71±8.47 gm./day respectively. Lambs birth weight was significantly influenced by ewes age, ewes aged 4 and 5 years got heavier lambs than those aged 1 and 3 years.7 - Lactoferrin genotypes significantly affected protein and total solids not fat, as AA genotype exceeded the other genotypes. Fat of AA, AB and BB genotypes were 2.85, 2.23 and 2.63 kg respectively. while values of SNF were 7.24, 5.78 and 7.51 respectively.8 - There was arithmetic increase (not significant) in all traits for the AB genotype over AA genotype of prolactin gene.9 - Allele B of prolactin gene positively and significantly influenced most trait. It shared about 75% of each trait variation. That of lactoferrin gene shared about 25% only.10 - Heritability of SNF% and quantity was high (0.652 and 0.666 respectively). Those of birth weight and growth rate were medium (0.309 and 0.346). All other traits had low heritability.11 - Highest value of BLUP was recorded for daily milk yield, total milk yield and adjusted milk yield for 150 days (0.032, 3.072 and 2.220 kg respectively). The lowest values were - 0.021, - 2.071 and 0.00 kg respectively. Their ranges were 0.053, 5.143 and 2.220 kg respectively.12 - The lowest value of BLUP of fat%, protein% and SNF% were - 1.724, - 2.448 and - 4.565% respectively. the highest values were 2.058, 2.466 and 3.764% respectively with a range of 3.782, 4.914 and 8.329% respectively.13 - The lowest values of BLUP of birth and weaning weight and growth rate were 3.00 kg, 19.9 kg and - 1.20 g/day, the highest were 5.5 kg, 23.45 kg and 1.03 g/day with ranges 2.5 kg, 3.86 kg and 2.23 g/day respectively.14 - Breeding values of prolactin AB genotype of all studied traits exceeded those of AA genotype. However, those of lactoferrin AB and BB genotypes exceeded those of AA genotypes.

التعبير الجيني ووفرة نسخ mRNA لجينات نقل العناصر الغذائية في الامعاء الدقيقة لفروج اللحم باختلاف مستوى الطاقة والبروتين في العليقة == Gene Expression and mRNA Abundance of Nutrients Transporter Genes in The Small Intestine of Broiler of Different Levels of Protein and Energy in Diets

اسم المؤلف: علي احمد عبد الكریم ابراهیم العامري
اسم المشرف: اسعد یحیى عاید | عبد لله عبد المنعم
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: This study was conducted in both Poultry Farm/Animal Resources Department/ College of Agriculture / University of Basra and laboratories of Molecular Biology/ London/ United Kingdom (The Molecular Oncology Unit Laboratories of Guy's and St Thomas's Hospital/ Kings College London/ UK). Molecular analysis of the samples and the detection of a number of gene expression of several feed transporter genes in the small intestine of broiler chickens when fed diets with different levels of energy and protein. A total of 225 unsexed one day old broiler chicks (Ross breed) were randomly distributed on five experimental treatments each with three replicates of 15 chicks. Feeding was ad libitum during the whole period of experiment that lasted 35 days.The first treatment was control with 22.3 and 20 % crude protein and 2910 and 3001 kcal/kg for starter and growth period respectively. The second treatment consisted 24.1 and 22 % crude protein and 2920 and 2978 kcal/kg for starter and growth period respectively. The third treatment consisted 20 and 18 % and 2898 and 2996 kcal/ kg for starter and growth period respectively. The fourth treatment consisted 21.9 and 20 % and 3107 and 3199 kcal/ kg for starter and growth period respectively. The fifth treatment consisted 22.2 and 20 % and 2720 and 2800 kcal/ kg for starter and growth period respectively. The results revealed the followings : - The study showed the ability to amplify all genes and find their abundance according to their primers. - The highest value of APN gene abundance of female in the duodenum (1.86 ng) fed diet with 22 % cp and 3199 kcal/kg ME. - Highest abundance (6.19 ng) of Pept1 mRNA showed by males in their jejunum, when fed a diet consisted 22 % cp and 3199 kcal/kg ME. - The greatest value of b - AT and CAT2 amino acids transporter genes effect was revealed by males fed diet with 22 % cp and 3001 kcal/kg ME. - Females fed 20 % cp and 3199 kcal/kg ME diets reordered highest SGLT1 gene expression (4.70ng) within their ileum. - Males fed 20 % cp and 3199 kcal/kg ME diets reordered highest GLUT5 gene expression (6.37 ng) within their ileum. - All genes associated positively and significantly with body weight, growth rate, carcass cuts percentages (legs, breast, intestine length and weight) at the age of 35 days and negatively with feed consumption and feed conversion ratio. - Amino acid and peptides transporter genes abundance improved with an increase in diet protein, that of saccharide transporter improves with the increase of ME level in the diets. - Male gave higher mRNA copies of most genes in comparison with females, except that of SGLT1 and NPA which increased in females. - It is possible to manipulate nutrient transporter gene expression by feeding. That may reflect on birds performance.

تاثير فاصلة ومعاملات الري وتغطية سطح التربة في بعض خصائصها وانتاج نخلة التمر Phoenix dactylifera L. == Effect of interval and methods irrigation in addition soil surface mulching on some soil properties and production of date palm Phoenix dactylifera L

اسم المؤلف: عبد الرحمن داود صالح الحمد
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: التربة والمياه
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The study was conducted in an agricultural field which located at Abu - AlKhaseeb distruct /20 Km south of Basra City , during the season of growth (2013 and 2014) on a land area about one hectare, a clay soil texture and classified as Typic torrifluvents. In order to study the effect of method and interval irrigation and mulching soil surface in some physical and chemical properties of the soil and production of date palm Phoenix dactylifera L. Hillawi c.v. The irrigation method factor included four treatments; drip irrigation method (D) and surface irrigation (S) and rotation method (DS) (rotation drip irrigation with surface irrigation in binary cycle) and tide irrigation method (T), while the mulching factor included three treatments : nylon (N) and coverage waste (W) and no mulching (no). Irrigation interval factor included three treatments are (4 ,8, 12) day. Experiment carried out in factorial Experiment with random complete block design (RCBD) with three replications, the addition of irrigation water calculated from the basin American evaporation (Evap.Pan Class - A - ), which was erected in the study site, plus 20% as leaching requirements.Below is a summary of the study results : 1 - The addition of irrigation water by the irrigation methods (D, DS, S) increase the moisture content significantly for different depths of soil compared with traditional irrigation treatment (tidal). Surface irrigation treatment shows higher values in the moisture content, followed by treatment of irrigation alternately then treated drip irrigation, irrigation with treated showed tidal lowest values. The irrigation methods (drip, drip - surface, surface) increased in moisture content values with lack of a period irrigation, especially when the interval 4 day. Moisture content of the irrigation methods treatments increased significantly values morally using coverage, particularly nylon except irrigation treatment tidal which were not increase significantly. The moisture content values increase significantly with depth for all experimental treatments, and she took to decline at the end of the first season and the second compared to the first season.2 - The treatment of traditional irrigation (tidal) showed the lowest values in the bulk density, followed by treatment of drip irrigation and treatment of rotation and then surface irrigation treatment for all depths .The results showed increased bulk density values of all treatments with depth ,also that the use of the nearby interval and coverage nylon contributed to maintaining the values of bulk density and showed superior in giving the lowest value for the density. Increased bulk density values with the progress of seasonal growth of all treatments and three depths of soil.3 - The use of irrigation methods (drip, drip - surface, surface) to a significant decrease in the values of Mean Weighted Diameter (MWD) compared with the traditional method of irrigation (tidal) and the order of the effect for the irrigation methods is S <DS <D <T. The results showed that values increase with the progress of the growing season, except for the treatment of traditional irrigation (T) with superiority of depth (0 - 30) cm on other depths.4 - The cumulative infiltration and infiltration rate Increased in the soil with the traditional irrigation treatment compared to using other methods of irrigation and the methods irrigation taken following order in the cumulative infiltration and infiltration rateS <DS <D <T. The results showed that the cumulative infiltration and infiltration rate values increased with using mulching especially with nylon and follow short - rang irrigation interval .5 - The use of irrigation methods (drip, drip - surface, surface) decreased the electrical conductivity values on different depths of soil compared with traditional irrigation treatment (tidal), which maintained a significant increase in the electrical conductivity values, especially in (0 - 30 ,30 - 60 ,) cm depth, followed by treatment of drip irrigation and surface irrigation, The rotation treatment DS showed high efficiency in salts washing rate in all depths and recorded the lowest values in the electrical conductivity .The results showed that the using of mulching, especially nylon contributed significantly in reducing the electrical conductivity values, and that the exposed treatment showed higher values, as the results showed that the using of long interval increase in the electrical conductivity values .Other than increase the electrical conductivity values of the surface depth of all treatments and it decreased significantly at both ends of the first and second seasons, compared with the beginning of the experiment except for the treatment of tidal which remained conservative values until the end of the experiment .6 - The use of irrigation methods (drip, rotation, surface) contributed to the significant increase of the values of productivity (weight, size, length, total sugars, dry weight and total yield) of Date Palm compared with the use of traditional irrigation method (tidal ) .The irrigation methods takes the following order in productivity : T <S <D <DS. The results showed that a significant increase in productivity with use short interval and the use of nylon mulching style. The results showed that increase in productivity in the second season compared to the first season.

معايرة معادلات التبخر نتح المرجعي لمنطقتي الفرات الاوسط وجنوب العراق وتاثير جدولة الري والتسميد في بعض خصائص التربة الفيزيائية وكفاءة استعمال المياه لمحصول زهرة الشمس Helianthus annuus L) == Calibration of Reference Evapotranspiration Equations in the Midmost Euphrates Region and Southern Iraq and the effect of irrigation schedule and fertilization on some Soil physical properties and water - use efficiency of sunflower crop (Helianthus annuu

اسم المؤلف: طالب صبر حريجة العسكري
اسم المشرف: داخل راضي نديوي | محمد عبد الله عبد الكريم
الموضوع العام: علوم الزراعة
السنة: 2015
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Theoretical foundations for research included the estimations of reference evapotranspiration (ETo) in the midmost Euphrates region and southern Iraq by applying seven mathematical models as well as CROPWAT 8.0 and CLIMWAT software using two sets of climatic data for periods 1970 - 2002 and 2004 - 2010.The results could be summarized as follow : - 1. The annual mean of ETo calculated by the Penman - Monteith equation (PM) for the meteorological stations of Nasiriya, Amarah, Semawa, Najaf, Diwaniya and Kerballa were (6.00, 6.20, 5.92,5.97,6.13,5.74) mm day - 1 respectively, during the first measurement period, and (6.47, 6.08, 5.86, 5.98, 6.13, 5.65) mm day - 1 respectively, during the second period. Also, the ETo estimated by CROPWAT 8.0 and imported by CLIMWAT software have showed the same trend of PM calculations with overestimate reached (6.10, 5.93) % during the first and secondperiods for CROPWAT 8.0 software, and underestimate for ETo reached 8.16 %during the first period for CLIMWAT software compared to PM calculations.2. The statistical criterions for evaluating the performance of six empiricalequations of the original versions were compared with the Penman - Monteithequation revealed that the variations in their performance with relativelypreference for temperature - based equations in comparison with radiation - basedequations. Blancy - Criddle (1950) equation (BC) has proved superiorperformance in Nasiriya, Semawa, Najaf, Diwaniya and Kerballa stations duringboth periods, also in Amarah station during the second period and declining itsperformance for the benefit of Kharrufa equation (1985) (K) during the firstperiod of measurement.3. The calibration process of the six empirical equations including : Hargreaves - Samani (1985) (HS), Makkink (1957) (M) , Priestley - Taylor (1972)(PT),andHargreaves (1975) (H) in addition to BC and K equations were made bysubstituting recalibrated constant values for the original constant values afterthese constant had modified to fit PM calculations and it used a set of statisticalcriterions to verify the validity of calibration and its ability to reduce theestimates errors compared to the original formulas. As the indices ofconcordance (D), confidence (C), correlation (r) and regression (R2) togetherwith the Nash - Sutcliffe Efficiency(NSE), standard deviation ratio (RSR). meanabsolute error (MAE), root mean square error (RMSE), relative error (RE)indicators and the ratio between both average estimations of ETo (r) are used toassess the validation of calibration and optimization which revealed thatconfidence became high in modified equations with reducing the estimation errorof ETo due to approach the values of these criterions from optimal value, then,they had improved efficiency of the six equations to simulate the values of EToare more accuracy than original formulae.4. The proposed new constants for the estimation of the ETo with : BC was 1.37for the time periods from January to April and 1.39 for May to December insteadof the original constant 1 : 41, HS was for the time periods from Octoberto March and for April to September instead of the original constantb0.0023, K was for June - July and for remained months instead of theoriginal constant 0.34, M are for the time periods from November to Mayand for June to October instead of the original constant 0.61, the equationsPT and H were 1.96 and 0 0.0165 for the period of December - May and 2.61and 0.0178 for the period from June to November instead of the originalconstants of 1.26 and 0.0135 respectively and equationsThe side applied to the study has included the implementation of the fieldexperiment for the cultivation of sunflower crop through the loop spring 2013 inprivate farm lies according to the geographical positioning system (GPS) withinthe coordinates (606,366.3437642), (606320, 3437729), (606353, 3437749),(606400, 3437660), is located in Batah town, Thi Qar province, at a distance of 17km west of the city of Nasiriyah, up 5 meters from the sea surface. The mainobjective of experiment included the following : i. Measuring ETo for the study through the crop growing seasonii. To determine the crop coefficient (Kc) and the actual water consumption(ETa) of sunflower crop in the Batah areaiii. . To asses effect of irrigation scheduling and fertilizer application as well astheir combinations on the growth indicators, nutrient uptake, dry matter,crop yields, the crop water use efficiency and water productivity.iv. Evaluating impact of irrigation scheduling and fertilizer application besidestheir combinations on some of soil physical properties at the end season.A field experiment was carried out using factorial experiment in a randomizedcomplete block design (RCBD) Layout with three replications in two factors, nineirrigation Scheduling treatments were assigned to the main plots and four fertilizertreatments were assigned to the subplots. Sunflower seeds (flame) were planted onMarch 23 with plant density 61538 plant ha - 1; the harvest operation was conductedon 18 / July / 2013.The irrigation Scheduling was achieved based on replenish root zone to theborders of field capacity when it reach the percentage of management allowabledepletion (MAD) identified about 55% of total available water (TAW), taking intoaccount the diversity of the effective depth of the root zone according to the stagesof growth, . The experiment involved Irrigation scheduling of the sunflower basedby calculating ETa using Kc which adjusted in situ multiplying by ETo thatestimated by PM equation (S1) ,original BC equation (S2) and adjusted K equation(S3) using meteorological data for real time that collected from agrometeorologicalstation nearby the field. PM equation (S6) using historicalmeteorological data and from pan evaporation with applied (FAO 56, 1998) tocalculate pan coefficient (S4). Whereas, irrigation Scheduling based on soil waterbalance according to daily measurements of soil moisture and adjusted daily onthe basis of changes in moisture content (soil water balance) (S5), fixed irrigationinterval and variable irrigation depth (S7), different irrigation interval and variableirrigation depth (S8) and soil water balance for bare soil (S9). Also the studyincluded four fertilization treatments were used ( F : mineral fertilizer, C : Compost,M : mineral fertilizer + Compost , N : non fertilization). The results showed thefollow : c1. The maximum value of ETo during season growth of sunflower crops accordingto field measurements has increased significantly when using evaporation pan,which gave the highest average rate of 8.81 mm day - 1 in comparison with PMequation that recorded the lowest value of ETo with mean equal to 8.00 mm day - 1,while BC and K gave 8.66 and 8.14 mm day - 1 respectively.2. The converged seasonal mean of Kc value for sunflower crops during growthseason were 0.864, 0.862, 0.863, 0.861, 0.862, 0.862, 0.861, 0.862 for S1 - S8treatments respectively, while the Kc varied in different stages of growth, thehigher value of Kc was 1.213 observed during the flowering stage, followed bytwo phases of vegetative growth and maturity with mean 0.796, 0.494respectively, whereas the lowest values (0.350) was recorded in the initial stage3. The ETa values were variable with various irrigation Scheduling treatments,the treatments (S1 - S8) gave mean ( 876.86, 938.98, 885.57, 943.40, 898.47,949.05, 835.61, 839.76) mm Season - 1 respectively, the mean values of ETa aredistributed as 52.28% during flowering stage, 23.38% through maturity, 18.97%during vegetative growth stage and 5.36% in the initial stage4. The Irrigation scheduling of the sunflower based on S5 treatment caused toincrement of water consumption by transpiration during flowering stage to reach83% from total ETa depth, whereas The Irrigation scheduling based on S7treatment caused to increment of water consumption by evaporation during thesame stage to reach 29% from total ETa depth.5. The results demonstrated that all Irrigation scheduling have been showed astrong relationship with water balance measurement (S5), by R2 is greater than0.91 and r greater than 0.95, also, the results showed there was a preference to therevised K equation to calculate ETa of sunflower crops in the region according toindicators of regression model each of the R2, slope and intercept which amountedto 0.9395, 0.9875, - 0.0144 respectively.6. The overall mean of net irrigation depth was 874.6 mm season - 1 ranged from941 mm season - 1 for S4 treatment to 800 mm season - 1 for S7 . the addition ofwater was 14 applications for S1 - S4 treatments and 15, 13, 12 applications for S5,S8, S7 respectively.7. The lowest nitrogen concentration in the leaves of Sunflower crops duringflowering stage related to decrease water supply at S7 (27.69 g kg - 1) , then, itincreased to maximum value ( 31.48 g kg - 1) when irrigation Scheduling based onS1 followed by S3, S5, S2, S4 treatments,. As respect with maturation stages theS5 treatment gave maximum mean (26.81 g kg - 1) was significantly superiorcompared to all treatments except S1 and it gave increment in nitrogenconcentration equal to 22% in comparison with S8 which recorded lowest nitrogenconcentration (21.94 g kg - 1). The results showed the F treatment was significantlysuperior followed by M treatment which gave average 34.43, 33.42 g kg - 1 duringflowering stage and 28.69, 26.48 g kg - 1 during maturation stage respectively.8. Increasing irrigation depth due to an application S4 and S6 treatments andIncreasing the irrigation frequency for S5 treatment led to significant increment inthe bulk density for 0 - 15 cm depth with mean 1.393, 1.384, 1.386 M m - 3respectively, the bulk density decreased steadily with decreasing of irrigationddepth and irrigation interval until reach their lowest level (1.350 M m - 3) at S7treatment. The bulk density was significantly decreasing when C and M treatmentswere applied with means of equal to (1.362, 1.364) M m - 3 respectively, atmagnitude 1% higher than their initial values. Also the results showed theirrigation scheduling treatments and fertilizer application as well as theircombinations failed to exhibit any significant influence on bulk density for depths15 - 30 cm and 30 - 45 cm.9. The results showed a significant increase in the soil penetration resistance forsoil with high watery supply (S4 and S6), while that status is significantlydecreased under treatment of low watery supply (S7, S8 and S1), the soils withmedium watery supply causing the pattern moderate between the two, also, thisepithet is significantly reduced when you add compost with an average of 708.09kPa, while there was a significant increase resulted from control treatment andaddition of chemical fertilizer alone, the proportion of 31.81 and 18.41%sequentially compared with the addition of compost.10. The results showed the possibility basic infiltration rate for most treatmentsunder the category (moderately slow) according to Landor (1991), by the mean ofbasic infiltration rate was 1. cm h - 1 ranged between 0. to 3. cm h - 1, whereasbasic infiltration rate for S4N and S9 decreased to 0.6 , 0.51 cm h - 1 respectively,thus it can be classified into (slow) while it increased greater than 2.0 cm h - 1 forS1C, S1M, S2M, S5C, S7F, S7M,S7C, S8M treatments which refer to moderateinfiltration rate according to the same classification . C and M fertilization led toimprove soil physical properties, which in turn positively affected the infiltrationrate with increment in magnitude (5 , 56) % respectively, compared to Ftreatment.11. Treatment of F gave a significant superior in most morphologicalcharacteristics and yield components, the efficiency of F treatment for productionwas 37.45 % followed by M and C treatments were achieved 24.66, 19.24 %respectively, compared to N treatment.12. Treatment F achieved highest yield of grains 1945.80 kg ha - 1, an increasing was24.32% in comparison with N treatment which gave lowest yield of grains reached1367.24 kg ha - 1, also there were a significant increase in crop water use efficiencyand water productivity are associated with application of F and M fertilization13. The productivity characteristics of sunflower were significantly affected byirrigation Scheduling treatments, The highest seed yield 1893.8 kg ha - 1and thehighest dry matter 5246.2 kg ha - 1were obtained under S5 treatment, while the S7treatment recorded lowest values of these indicators (1512.5 and 3765.4) kg ha - 1 respectively,14. The application of Irrigation scheduling based on S5, S1 and S3 treatments achieved a significant increase in crop water use efficiency and water productivity, the mean of first parameter was (0.219, 0.216, 0.216) kg m - 3 respectively, and themean of the second parameter was (0.228, 0.220, 0.219) kg m - 3 respectively.

تاثير انظمة الاضاءة والتقنين الغذائي الزمني في بعض الصفات الانتاجية والفسلجية والتناسلية لطائر السمان الياباني (Coturnix japonica) == The Effect of Lighting Systems and Feed Restriction timing on Some Productive, Physiological and Reproductive Traits of Japanese Quail (Coturnix japonica)

اسم المؤلف: صباح كاظم مرزوق الحمود
اسم المشرف: ریاض كاظم موسى | ربیعة جدوع عباس
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:

عزل وتشخيص بكتريا Bacillus cereus واستعمالها في انتاج متعدد هيدروكسي البيوترات == Isolation and Identification of Bacillus cereus Bacteria and Using in Polyhydroxybutyrate Productio

اسم المؤلف: شيماء ذياب جدوع السهلاني
اسم المشرف: امال كاظم غضبان الاسدي
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الاغذية
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Forty three local bacterial isolates were obtained after heating treatment for different sources collected from many places in Basra government. Included : Vegetables, Fruit, Pastry (cakes), Legumes, Soils, Sand, Animal manure, the papyrus plant, milk, water liquefaction and sewage water. Primary screening for the isolates by Sudan black B dye only. 22 isolates were chosen with strong staining, and the identification of all the isolates revealed were belong to genus Bacillus by studying microscopic and biochemical tests. They were : 4 isolates Bacillus cereus, 2 isolates Bacillus firmus, 2 isolates Bacillus lichenformis , 3 isolates Bacillus megaterium, 4 isolates Bacillus mycoides , 3 isolates Bacillus pumilus and 4 isolates Bacillus subtilis.Secondary screening of these isolates was Polyhydroxybutyrate (PHB) produced, Bacillus cereus B5 given 2.4 g / L the highest production of PHB. A PCR technique was used for 16S rRNA test and detecting the gene of PHB production in Bacillus cereus B5.The highest PHB production from Bacillus cereus B5 was 6.2 g / L, biomass 8.4 g / L and yield 73.8% the by using optimum conditions : incubation temperature 35̊ C, for 48 hours aerobically by using shaking incubator for 150 RPM/ min, 2% inoculum volume, the pH was adjusted to 7, and the production media where contained 1% glucose as carbon source and peptone as a nitrogen source. 3% have dated juice was the best substitute for glucose as carbon source, it gave 7.11 g PHB / L and the yield 79 %.Analysis with FT - IR was showed, that PHB produced from date juice media had a peak at 1723 cm - 1 this means it belong to an esterpolymers group. And GC - MS showed 12 compounds produce from analyzing PHB as short chains of fatty acids.The properties of PHB produced from date juice media were : The degradation temperature was 312̊ C, the percentage of crystallization was 60%, molecular weight was 423.674 KDa., permeability of water vapor was 56.2 ×10 - 8 g / Pa.s.m2, tensile strength was 24.9 MPa, the blend 40% polyethyleneglycol as plasticized with PHB increased the percentage of elongation to 8.7%. PHB is non - toxic and without hemolysis on human blood.Studying biodegradation of the plastic films of PHB by using bacterial isolates were E. coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus in petri dish showed that all isolates can degrade PHB, biodegradation in soil and at soil surface was 100% after 28 days.PHB packages increased the shelf life of strawberries and grapes after storage for 15 days, compared with polyethylene packages, and It was reduced the numbers of microorganisms in butter, the percentage of free fatty acids and peroxide value compared with butter with polyethylene after 15 days

تاثير اضافة النتروجين في امتصاص NوPوK وتوزيعها في اجزاء النبات ونمو وحاصل ثلاثة اصناف من الحنطة Triticum aestivum L. == EFFECT OF NITROGEN APPLCATION ON N, P, K UPTAKE AND DISTRIBUTION WHIHIN PLANT PARTS, GROWTH AND YIELD OF THREE WHEAT CULTIVARS Triticum aestivum L.

اسم المؤلف: سندس عبد الكريم محمد العبد الله
اسم المشرف: عبد المهدي صالح حسين الانصاري | وليد عبد الرضا السباهي
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: الاغذية
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: A field experiment was conducted at Al - Daire site 40 km north of center Basrah Governorate .The study was conducted to investigate the effects of different nitrogen fertilizer levels on dry matter accumulation, (N, P, K) uptake, distribution in different parts of three cultivars of wheat plants and their yield during two seasons (2011 - 2012 & 2012 - 2013).The experiment was split - plots in R.C.B.D design with three replicates, Nitrogen rates (0, 60,120 and 240Kg ha - 1 occupied the main plots, while (IPA - 99, Abu - Graib and Latifia) Cultivars Occupied the sub - plots. Samples of wheat plants were collected at 10 growth stages : seedling stage, tillering (main shoot and 1 tiller), tillering (main shoot and 4 tillers), stem elongation, booting, 50% of spike emergence, 50% flowering, milk stage, dough stage and maturity. Each plant sample was separated into different plant parts : blades, sheathes, stems, flag leaves, spikes and seeds. These parts were dried at 70°C and dry weight of each part was recorded and sub sampled for chemical analysis of N , P , and K. Growth parameters (No. of day till 50% flowering , No. of day from 50% flowering to Maturity , flag leaf area , plant height, spike length and No of tillers m - 2) , yield components (No. of spike m - 2, No. of grain in spike and weight of 1000 grain ),grain yield, biological yield, harvest Index, grain protein percentage and nitrogen use efficiency were also studied.Results showed that dry matter accumulation in plants of all cultivars did not differ at early growth period, While after the growth stage of 50% flowering plants dry weight of Cv.IPA - 99 was higher than other cultivars, This difference in dry weight continue till maturity stage at which total dry weight were 14473.58, 13373.96 and 12743.59 Kg ha - 1 for IPA - 99, Abu - Graib - 3 and Latifia respectively.Dry matter yield were not affected by N levels during the early growth period ,then after dry weight of plants increased as N rates increased ,total dry weight were10752.61, 12737.49 and 15347.10 Kg ha - 1for 0, 60 , 120 Kg Nha - 1 respectively, with no differences between 120 and 240 Kg Nha - 1 levels.Dry matter of plant parts differed among cultivars, with IPA - 99 having the highest results. Increasing nitrogen rates increased weight of different plant parts, but did not influence the relative proportion of each plant part for all cultivars.IIResults also showed reduces in dry weight of different plant parts(except grains)after reaching a maximum weigh at dough stage for Spikes, and 50% flowering for the other parts. The highest decreased was in blades and lowest in sheathes. The application of N fertilizer decreased translocation of dry matter from plant parts to grains. Data showed that no differences in nutrient uptake were observed for cultivars till ending of tillering stage, but after this stage IPA - 99 cultivar showed highest uptake till the end of the season. Nitrogen uptake was157.68,136.72,124.51 Kgha - 1 ,P uptake 19.70,16.93,15,67 Kg ha - 1, K uptake136.30,121.10,112,60 Kg ha - 1 for IPA - 99,Abu - Graib - 3 and Latifia, respectively.Increasing N rate increased nutrient uptake of different plant parts, but did not influence the relative proportion of each plant part for all cultivars.uptake of N, P, and K for all cultivars and nitrogen rates declined as the season progressed, this decline differed among nutrients and plant parts.Grain contained 59.20, 79.00 and 7.45% of total nutrient N, P and K uptake, whereas vegetative parts contained 40.80, 21.00 and 92.55% of total nutrient uptake, respectively.Cultivar IPA - 99 resulted in highest grain yield among studied cultivars. Increasing N rate from 0 to 120 Kg N ha - 1 only increased grain yield, the rate 240 KgNha - 1 did not affect yield significantly as compared with that of 120 Kg N ha - 1.Results also showed a significant interaction between Cultivars and N rates. The highest grain yield obtained at treatment IPA - 99×240 Kg N ha - 1with no significant differences than IPA - 99×120 KgNha - 1.Results indicated that highest nitrogen use efficiency (66.23%) was found in plant of IPA - 99 cultivar, whereas, the lowest efficiency (47.37%) was found in plant of Abu - Graib - 3 cultivar. At all cultivars increasing N rates to 240 KgNha - 1 decreased nitrogen use efficiency.Therefore it can be concluded that 120 Kg Nha - 1 can be recommended as the best rate for N to all cultivars under similar growing conditions

اثر اضافة الكبريت الزراعي واليوريا والسوبر فوسفات في نشاط انزيمي اليوريز والفوسفاتيز وحاصل الذرة البيضاء Sorghum bicolor L. == Effect of Agricultural Sulfur , Urea and TSP application on the activity Of Urease phosphatase and yield of (Sorghum bicolor L. )

اسم المؤلف: رشاد عادل عمران الزبيدي
اسم المشرف: هيفاء جاسم حسين التميمي | عبد الزهرة طه ظاهر
الموضوع العام: علوم الزراعة
السنة: 2015
الموضوع الدقيق: التربة والمياه - الخصوبة
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
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