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تشخيص فطري Trichoderma viride وTrichoderma harzianum بتقنية PCR واستخدامهما في تحسين القيمة الغذائية لمتبن واداء الحملان العرابية == Identification ofTrichodermaviride andTrichodermaharzianum by using Molecular Technique and Used to ImproveFeeding Value of Straw and Performance of Arabi Lambs
Author name:
ميثم خلف علي الغالبي
Supervisor name:
عماد فلاح حسن | طالب احمد جايد
General topic:
Agricultural sciences
Specific topic:
Zoology
Degree:
Doctorate
University:
University Of Basrah - Faculty Of Agriculture
Language:
Arabic
University location:
Basrah
First pages:
31T1895 - p.pdf
Abstract:
The objectives of current study were to identify Trichoderma viridi and Trichoderma harzianum by genetics methods and used them singly or both (3% for each treatment) in improvement of barley straw and animal performance. A total of 20Arabi lambs aged about 3 - 4months with an average weight of 22.725±1.43 kg distributed randomly and equally to four nutritional treatments. Lambs fed individually as 2% of body weight, while straw was given ad libtium. The results obtained can be summarized as follows : 1 - The study was able to identify TV and TH using three different PCR techniques, which are PCR - RAPD, PCR - ITS and PCR - IGS.TV and TH showed three and two bands respectively when using PCR - RAPD at nearly 600bp.While both fungi showed one band by using PCR - ITS and PCR - IGS and at nearly 600 and 2,400bp respectively.2 - Using both fungi (treatment 4) resulted in a significant improvement in the chemical composition of the straw through the high content of crude protein and low crude fiber, ADF and NDF compared to untreated straw.3 - There were improvements indigestibilityofcrude protein, fiber, cellulose, hemicellulose, ADFand NDF when the both fungi treatment was used. As well as total digestible nutrients of the fungus treated groups in comparison with untreated straw (control group).4 - Feed intake of treated straw groups increased significantly compared to untreated straw groupwiththe superiority of the group that was treated with both fungi (639.91 - 826.30 gm/head/day for control and both fungi group respectively). As well as there wasa significant improvement in feed conversion efficiency of a group treated with both fungi in comparison of other groups (6.38 - 5.52 kg feed/kg growth rate respectively).5 - Rumen pH, ammonia and total volatile fatty acid of lambs fed treated straw (6.22 - 6.89, 10.00 - 18.69; 11.35 - 13.61 for control115group and both fungi group respectively) were significantly higher than those of untreated group.6 - There were significant improvements in lambs’bodyweigh tsand their absolute and relative gains when fed treated straw (37.82 kg, 14.97kg and 65.67% respectively) especially the group fed both fungi in comparison with untreated group (32.79 kg, 10.03kg and 43.89%respectively).7 - Globulin, AST and ALT was not significantly influenced bytreated straw. On the contrary, there were a significant increase inhemoglobin, glucose, total protein, albumin and albumin/globulin ratio of the treated groupcompared with the untreated straw. While a significant decrease in urea and cholesterollevelin theblood serumof treated groups in comparison with untreated group.8 - Economic evaluation ofadding fungitostrawshowed improvement inthe relativeeconomic efficiencyby26.2%, 37.0% and 59.5% for adding TVorTH or bothtogetherrespectively. Adding fungi decreasedthe cost of feed from 46.65Dinars/ kg increase in body weight gains incase of untreated straw to 40.15dinars/ kg when adding both fungi.
