عرض: 25 50 75 100 النتائج

نتائج البحث: 25 من أصل 1,162

الصيرفة الاسلامية واثرها في انشاء سوق مالية اسلامية == Islamic Banking and its effect on establishing an Islamic financial market

اسم المؤلف: جمال مهدي صالح
اسم المشرف: احمد سامي شوكت | اركان يوسف حالوب
الموضوع العام: الادارة والاقتصاد
السنة: 2017
الموضوع الدقيق: الاقتصاد الاسلامي
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: These studies focus on several aspects of Islamic banking and the challenges it faces in the context of the contemporary reality in which it is operating. It deals with the state of the market and the transactions taking place in it, and the role of banks in the establishment and development of the Islamic financial market; It is known that the financial markets are revolving with banks and non - existent, money markets are one of the most important areas developed in dealing with all the channels in which money flows between different sectors of society and its members, and is concerned with the banking industry aimed at developing Find alternatives, and address developments.Since the success and evolution of the financial markets, depends closely on the diversity and evolution of the financial instruments in circulation; it is called for : intensifying efforts, coordinating them and promoting the development and creation of Islamic financial instruments, in accordance with Sharia law. , which expands and activates the Islamic financial market; It is activated and activated. It complements and contributes to the development of Islamic banking, on the one hand, and works to boost economic growth in Islamic countries on the other.As the local banking arena is witnessing, regional, and international, is a huge development in the Islamic banking industry, in the form of the creation of Islamic banks, or by turning some banks into Islamic banking, as well as providing many Local and international banks of Islamic Banking services, besides the services of the traditional bank, this development in the Islamic banking market has been accompanied by a similar development in Islamic means of investment provided to customers in Islamic banks; to arrive Currently more than 20 investment instruments used by Islamic banks.Hence the need to highlight the role that Islamic banks can play in supporting Islamic financial markets. and development; it is one of the most important investment outlets that absorb its surplus liquidity and provide it with sufficient cash at Need, and fund their projects and ideas

احكام الفقه السياسي في ضوء السيرة النبوية == The provisions of political jurisprudence in the light of the Prophet's biography

اسم المؤلف: مهند سعد قاسم سعيد العبيدي
اسم المشرف: صلاح حميد عبد العيساوي
الموضوع العام: العلوم الاسلامية
السنة: 2017
الموضوع الدقيق: العلوم الاسلامية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:

دراسة التنميط الوراثي ل,HLA فيتامين دي 3 وبعض العوامل المناعية لمرض التدرن الرئوي في مدينة الحلة == Study of HLA : DRB1, Vitamin D3 and Some Immunological Factors in Pulmonary Tuberculosis in Hilla City

اسم المؤلف: اشواق مخيف سلمان الجبوري
اسم المشرف: محمد عبد كاظم حسن السعدي | هادي محمد علي الموسوي
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تم خلال الدراسة جمع عينات الدم من 50 مريضا بالتدرن الرئوي من المراجعات الى العيادة الاستشارية للامراض الصدرية في الحلة - محافظة بابل خلال الفترة مابين شهر شباط 2016 الى شباط 2017. ومن بين مرضى التدرن الرئوي، كان هناك 27 ذكور و23 اناث، وعمر المرضى يتراوح بين 12 - 80 عاما. خمسون شخصا من الاصحاء ظاهريا كمجموعة سيطرة لم يكن لديهم تاريخ من مرض التدرن الرئوي. جميع المرضى الذين يعانون من مرض السكري، السرطان، الحساسية والنساء الحوامل استبعدوا من الدراسة.تم استخدام SSO)) في تحديد مورث معقد التوافق النسجيDRB1 .HLA - تم قياس السايتوكينات وفيتامين D3باستخدام تقنية الاليزا.وفقا لنتائج هذه الدراسة، كان الذكور 54٪ بينما الاناث كان 46٪ من مرضى التدرن الرئوي. وكانت الفئات العمرية بين (12 - 35) سنة الفئة العمرية الاكثر ضعفا مع السل الرئوي سجلت (50٪). كانت مدة الاصابة بين (1 - 3) اشهر هي السائدة بين مرضى السل الرئوي والمرضى الذين دون علاج كانت سائدة بين مرضى السل الرئوي. وكان التوزيع الجغرافي لمرضى التدرن الرئوي اعلى بنسبة 62 % في المناطق الريفية من 38 % في المناطق الحضرية.وقد درس التوزيع الوراثي لمستضد كريات الدم البيضاء البشرية HLA - DRB1 في 50 مريضا مصابا بالتدرن الرئوي و50 من مجموعة السيطرة. كان HLA - DRB1 * 13 زيادة معنوية في مرضى التدرن الرئوي 48٪ بالمقارنة مع مجموعة السيطرة 16٪ في حين HLA - DRB1 * 15 كان زيادة معنوية في مجموعة السيطرة 24٪ بالمقارنة مع مرضى التدرن الرئوي 6٪.وجد مورث معقد التوافق النسجي HLA - DRB1alleles اكثر شيوعا في الاناث 77٪ من الذكور 23٪ من العدد الاجمالي للمرضى. ايضا، كان HLA - DRB1 * 13 اكثر شيوعا في الاناث 75٪ من الذكور 25٪ من مرضى التدرن الرئوي في حين كان HLA - DRB1 * 15 شائعا في الاناث 100٪ ولم يظهر في الذكور.اظهر متوسط تركيز مصل الدم IL - 10 ان هناك زيادة معنوية (p˂ 0.05) في مرضى التدرن الرئوي بالمقارنة مع مجموعة السيطرة (4.024 و1.049) بغ / مل على التوالي. كما اظهر متوسط تركيز مصل الدم من IL - 12 وجود زيادة معنوية (p ˂ 0.05) في مرضى التدرن الرئوي مقارنة بمجموعة السيطرة (51.563 و13.514) بغ / مل على التوالي.في حين لم يظهر متوسط تركيز مصل IFN - γ زيادة معنوية (p˃0.05) في مرضى التدرن الرئوي مقارنة بمجموعة السيطرة (36.505 و25.019) بغ / مل. اظهر متوسط تركيز مصل الدم ل VD3 انخفاضا معنويا (p ˂ 0.05) في مرضى التدرن الرئوي مقارنة بمجموعة السيطرة (18.186 و29.321) نغ / مل.

دالات بيوكيماوية جديدة للساركوبينيا والضعف العضلي لكبار السن في العراق العام الدراسي 2016 - 2017 == Novel Biochemical Markers for Sarcopenia and Muscle Frailty in Iraqi Elderly

اسم المؤلف: ولاء اسماعيل جاسم
اسم المشرف: هدف ظافر الياسين | نزارعبد اللطيف
الموضوع العام: الطب
السنة: 2017
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تعرف الساركوبينيا بانها النقصان التدريجي في قوة وكتلة العضلات الهيكلية وهذا يحصل بتقدم العمر. الساركوبينيا اما ان تكون ابتدائية سببها تقدم العمر فقط بدون وجود اي سبب اخر او ثانوية وسببها تقدم العمر الذي له علاقه بالفعالية , الامراض ولتغذية. العينات وطريقة العمل تم اخذ مجموعه عينات دم من اشخاص يرتادون المستشفى التعليمي في بغداد/قسم الروماتولوجي خلال الفترة من الاول من ايلول 2016 الى نهاية شباط 2017. تضمنت الدراسة 100 عينه لاشخاص يعانون من الساركوبينيا(50 امراه و50 رجل) و50 يبدون اصحاء كمجموعة سيطرة(25 امراه و25 رجل). اخذت المعلومات من جميع الاشخاص وقد تضمنت الجنس والعمر والاصابة بالامراض. ( استبعدالا شخاص الذين يعانون من الروماتيزم الرثوي, داء الذئبة الاحمراري، مرض السكري، امراض الغده الدرقية والاشخاص الذين يتعاطون ادويه تحتوي على ستيرويدات). تم تشخيص الاشخاص الذين يعانون من الساركوبينيا الابتدائية بواسطة اختبار الاداء الحركي لكبار السن وجهاز فحص هشاشة العظم (لتعيين مجموع كتلة عضلات الجسم وكتلة عضلات الهيكل العظمي، ومعامل كتلة الجسم. ). اجريت بعض التحاليل البيولوجية التي تضمنت تحاليل تخص الالتهابات مثل الانترلوكين - 6 والبروتين سي عالي الفعالية والالفا 1 انتي - كيموتربسين ولقياس تليفات العضلات (البروكولاجين تايب 3 ان ترمنل ببتايد) ولقياس التقاطع العضلي العصبي ( تركيز السي اكارين فركمنت )ولقياس نمو العضلات تم قياس المايوستاتين.كل المتغيرات البيولوجية قيست بواسطة استخدام تقنية الالايزا. النتائجمعدل قيم كل من كتلة عضلات الجسم والهيكل العظمي والالفا 1 انتي كيموتربسين في مجموعة السيطرة اعلى مما في مجموعة الساركوبينيا وفي الرجال اعلى من النساء وجميع القيم تقل بتقدم العمر. معدل قيم معامل كتلة الجسم ,الانترلوكين - 6 والبروتين سي عالي الفعالية، البروكولاجين تايب 3 ان ترمنل ببتايد وقياس تركيز السي اكارين فريكمنت والمايوستاتين في مجموعة السيطرة اقل من المجموعة المصابة بالساركوبينيا وفي النساء اعلى من الرجال وتزداد القيم بتقدم العمر. هنالك فروقات معنويه عالية في قيم جميع المتغيرات P≤0.01 بين مجموعة السيطره ومجموعة الساركوبينيهاما بالنسبة للفروقات المعنوية حسب الاعمار فهناك فروقات معنويه عالية بين كل المتغيرات البيولوجية P≤0.01ما عدا المايوستاتين حيث لا توجد فروقات معنويه بين قيمه P≥0.05اما بالنسبة للعلاقة بين المتغيرات اعتمادا على الجنس فهي ذات فروقات معنويه عالية P≤0.01 ماعدا قيم الفا 1انتي - كيموتربسين والسي اكارين فركمنت حيث كانت الفروفات معنويه P≤0.05 كما لم تكن هنالك فروقات معنويه بين البروكولاجين تايب 3 ان ترمنل ببتايد والسي اكارين فركمنت P≥0.05 المناقشه1 - معدل قيم كل من كتلة عضلات الجسم وكتله عضلات الهيكل العظمي والالفا 1 - كيموتربسين في مجموعة السيطرة اعلى مما في مجموعة الساركوبينيا وفي الرجال اعلى من النساء وجميع القيم تقل بتقدم العمر. السبب في ذلك قلة الالياف العضليه , قلة خلايا وحدة الحركه , قلةخلايا الارسال وزيادة كتلة الدهون في الاشخاص المصابين بالساركوبينيا. 2 - معدل قيم كل من الانترلوكين - 6 والسي بروتين عالي الفعالية يقلل من قوة العضلات في حين ان الالفا 1 - كيموتربسين يزيد من قوة العضلات عند الاشخاص المصابين بالساركوبينيا. والسبب في ذلك ان الالفا 1 - كيموتربسين تحمي العضلات من التلف اثناء الالتهابات ولكن زيادة معدل قيمة الانترلوكين - 6 يثبط عمل الالفا1 - كيموتربسين . وتزداد قيم السي بروتين عالي الفعاليه والانترلوكين - 6 بتقدم العمر وفي الرجال اكثر من النساء بسبب زيادة كتلة غضلات الجسم وكتلة عضلات الهيكل العظمي .3 - معدل قيم السي اكارين فركمنت في مجموعة الساركوبينيا اعلى منه في مجموغة السيطرة والسبب في ذلك عند اعادة بناء الخلايا العصبيه والعضليه عند كبار السن او المصابين بالساركوبينيا الاكارين ينقسم بواسطة انزيم الكيموتربسين ويطرح السي اكارين فركمنت الى الدم وتزداد فعالية الكيموتربسين وتقل قوة الخلايا العضليه والعصبيه.4 - معدل قيم البروكولاجين تايب 3 ان ترمنل ببتايد في مجموعة الساركوبينيا اعلى من مجموعة السيطرة والسبب انه خلال عملية اعادة بناء الخلايا العضليه العصبيه البروكولاجين تايب 3 ان ترمنل ببتايد قيمها ستزداد بسبب قلة الالياف العضلية وكذلك عملية ازالة التعصب مما يؤدي الى ضعف في الكتله العضليه.5 - المايوستاتين ينظم نمو العضلات سلبيا. المايوستاتين اساسا يثبط عمليه تصنيع المايوستاتين وعندما يزداد تركيز المايوستاتين يقل نمو وقوة العضلات.6 - في الاشخاص المصابين بالساركوبينيا معامل كتلة الجسم اعلى من مجموعة السيطرة. زيادة الدهون ووزن الجسم سببها هو قلة الاحتياج الى الطاقه, قلة فعالية الجسم, قله معدل الايض القاعدي واستخدام كمية سعرات اكثر من التي يحتاجها الجسم.7 - اكثر العوامل التي تؤثر على الساركوبينيا تباعا هي كتلة عضلات الجسم الهيكليه,كتلة عضلات الجسم, المايوستاتين, البروكولاجين تايب 3 ان ترمنل ببتايد , السي اكارين فركمنت ثم الالفا 1 - كيموتربسين. | Sarcopenia is defined as the progressive decline in skeletal muscle mass and strength that occurs with aging. Sarcopenia either primary which is age - related and with no other cause except ageing or secondary sarcopenia which is related to activity, disease and nutrition.Subjects and methodsA cross sectional, case - control study was done in Rheumatology Clinic in Baghdad Teaching Hospital (during the period from September first 2016 to the end of March 2017). It included 100 sarcopinic subjects (50 male and 50 female) and 50 non - sarcopinic subjects (25 male and 25 female). Information was taken from each subject including age, & gender and diseases. Patients with inflammatory diseases (rheumatoid arthritis, systemic lupus erythematosus), diabetes mellitus, thyroid disease and patients taking steroid therapy were excluded.Subjects with primary sarcopenia in this study were diagnosed by : Short Physical Performance Battery and dual - energy x - ray absorptiometry. appendicular skeletal muscle mass, total lean body mass and body mass index.The biomarkers studied were : inflammation : (interleukin - 6, hs - C - reactive protein and α1 - antichymotrypsin ), for markers of muscle fibrosis : (procollagen type III N - terminal peptide, for markers of functional neuromuscular junctions C - terminal Agrin fragment and myostatin

دراسة جزيئية للطفرتين NPM1 - A وFLT3 - ITD مع التعبير النسخي للمورث الجزيئي FLT3 في نموذج من المرضى العراقيين البالغين المصابين بابيضاض الدم النقياني الحاد وعلاقتهما مع المؤشرات السريرية والمختبرية == Molecular Study of NPM1 - A, FLT3 - ITD Mutations and FLT3 Transcript Expression in a Sample of Iraqi Adult Acute Myeloid Leukemia Patients : Their Correlations with Clinicopathological Parameters

اسم المؤلف: شيماء محمد عبد اللطيف
اسم المشرف: ختام رزاق كاظم الخفاجي | علي محمد جواد
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الامراض - الدم
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Acute myeloid leukemia is a hematological malignancy of myeloid progenitor cells characterized by anomalous proliferation, inhibition of differentiation and extension of leukemic cells blocked at the early stage of hematopoiesis. It has a great variability in the pathogenesis, clinical features, and treatment outcomes. Detection of molecular markers has become a smart tool to further division of patients in acute myeloid leukemia subgroups.Nucleophosmin1 mutations are found in approximately 30% of adult acute myeloid leukemia patients and are associated with a good outcome when detected in absence of duplications in the Fms - like tyrosine kinase 3 gene. Over 50 molecular Nucleophosmin1 mutation variants have been recognized; the most common one is Nucleophosmin1 - A mutation.The Fms - like tyrosine kinase 3 receptor is expressed on the surface of hematopoietic stem cells and plays a vital role in normal hematopoiesis. Numerous studies have revealed that high levels of Fms - like tyrosine kinase 3 were expressed in patients with acute myeloid leukemia. Fms - like tyrosine kinase 3 - Internal tandem duplication mutations are found in around 20 - 25% of patients with acute myeloid leukemia and are associated with increased transcript level of Fms - like tyrosine kinase 3 and with a poor scenario in adult acute myeloid leukemia patients.Aim of the Study1. Detect the frequency of Nucleophosmin1 - A; Fms - like tyrosine kinase 3 - Internal tandem duplication along with assessment of Fms - like tyrosine kinase 3 transcript expression in a sample of newly diagnosed Iraqi adult acute myeloid leukemia patients.2. Study the relationship of Nucleophosmin1 - A, Fms - like tyrosine kinase 3 - Internal tandem duplication mutations and Fms - like tyrosine kinase 3VItranscript expression with various clinicopathological parameters and French - American - British subtypes of the disease as well as the correlation among the three markers.Materials and MethodsThis is a cross - sectional study, conducted during the period extending between April 2015 and September 2016. The bone marrow aspirate samples of 53 adult acute myeloid leukemia patients were collected at the time of diagnosis and prior to treatment at the Hematology Ward of Baghdad Teaching Hospital in Medical City compared with 53 control individuals. All the control bone marrows obtained from patients with anemia and idiopathic thrombocytopenic purpura and were negative for infiltrative lesions. The related clinical and laboratory data for each patient were registered at the time of diagnosis.The study was conducted at Main Laboratory of Baghdad Teaching Hospital/ Medical City, Clinical and Communicable Diseases Research Center/College of Medicine/ University of Baghdad and Postgraduate Laboratory of Pathology and FoThe RNA was extracted and was reverse transcribed into cDNA. Real time polymerase chain reaction technique was applied on bone marrow aspirate samples of acute myeloid leukemia and control groups to detect the frequency of Nucleophosmin1 - A mutation, Fms - like tyrosine kinase 3 expression using a TaqMan probe and SYBR green assays respectively and detection of Fms - like tyrosine kinase 3 - Internal tandem duplication mutation using gel electrophoresis post polymerase chain reaction procedure

تاثير الفيتامينات B1,B6 and B12) على الاعتلال العصبي المحيطي : دراسة نسيجية، نسيجية كيميائية وكيميا نسيجية مناعية == The effect of vitamins B1, B6 and B12 on peripheral neuropathy , Histological , Histochemical and Immunohistological studies

اسم المؤلف: صالح مهدي علي
اسم المشرف: معن حمد الخالصي
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: التشريح والانسجة والاجنة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Peripheral neuropathy is a nerve injury process by disease or so on leading to damage of peripheral nervous system . Diabetes mellitus and compression are most common causes of peripheral neuropathy. Vitamins (B1,B6 and B12) commonly used for treatment of peripheral neuropathy. The objective of this study is to compare the effect of vitamins (B1,B6 and B12) on nerve regeneration and recovery after induced sciatic nerve crush injury in experimental animal model.Seventy five male Swiss albino rats were divided randomly into five groups and each group consisted of 15 animals. Group A( which considered as control positive(with crush injury) received normal saline intramuscularly for 45days. Group B received vitamin B1 , group C received vitamin B6 , group D received vitamin B12 and group E which considered as control negative(no crush injury and no treatment) . All animals were injected vitamins intramuscularly for 45days. Tail flick test time was reduced in rats treated with vitamin B12 after crush injury to the Sciatic nerve. Mid thigh circumference (MTC) was significantly increase in B12 treated group after crush injury to the Sciatic nerve. Data analysis was performed by SPSS 20 software using descriptive statistics.IVHistological examination of rats sciatic nerves stain by H&E on day 15 showing degenerative nerve fibers with Schwann cell infiltration in all groups but it is less prominent in group D. On day 45 histological examination of rats gastrocnemius muscles exhibited separation of bundles muscle fibers by connective tissue, variation of fibers in size and shape with atrophy of muscle bundles in all groups .While these were less prominent in rats treated with B12.Van Giesons staining of rat sciatic nerves on day 45 after crush injury. In all groups experimental and control (B,C,D,A) showing degenerative nerve fibers in different areas but less in group D and better organized sciatic nerve fibers in group D.Finally, Immunohistochemical study of (anti S100 protein) of rat sciatic nerves on day 45showed strong activity for those rats treated with vitamin B12 while rats treated with vitamin B1 and B6 exhibited moderate and weak activities respectively .In conclusion vitamin B12 promote peripheral nerve regeneration more than vitamin B1and vitamin B6.

الندرونيت الصوديوم في الفصال العظمي للمفصل الظنبوبي : تاثيره على معلمات بناء وانحلال الغضروف, لبتين والفعاليات الفيزيائية للمرضى العراقيين == ALENDRONATE SODIUM IN KNEE OSTEOARTHRITIS : EFFECTS ON ANABOLIC AND CARTILAGE DEGRADATIVE MARKERS, CIRCULATING LEPTIN AND THE CLINICAL ACTIVITY IN IRAQI PATIENTS

اسم المؤلف: سيناء عبد الامير كاظم
اسم المشرف: حيدر مهدي جواد | سامي سلمان شهاب
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الادوية والسموم
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: المقدمة : الفصال العظمي من الامراض الاكثر شيوعا في العالم والذي يصيب النساء اكثر من الرجال ويتميز هذا المرض بانه يحدث نتيجة لخلل في جميع مكونات المفصل متمثلا بانحلال الغضروف وتهشم العظم الملاصق له والتهاب الغلاف المحيط بالمفصل. متسببا في تقليل عرض الحيز الانسي للمفصل.الهدف من الدراسة : تقييم دور عقار الندرونيت الصوديوم ALN)) لمرضى الفصال العظمي للمفصل الظنبوبي ومعرفة تاثيره على مستوى الفعاليات الحياتية للمرضى التي تتمثل بمستوى الالم ومستوى تيبس المفصل وتاثيره على مستوى وظيفة المفصل الظنبوبي. وتقييم مدى تاثيره على معلم البناء 1β TGF , معلم انحلال الغضروف CTXII, مستوى لبتين في الدم, مايلوبيروكسيدييز MPO, الكالسيوم, مستويات الدهون بانواعه وفوسفات الالكلاين والبروتين التفاعلي C.طريقة العمل : ادخل 116 مريضا مصابا بمرض الفصال العظمي للمفصل الظنبوبي ضمن هذه الدراسة وكانوا بعمر من 45 سنة او اكثر وبالدرجة الثانية من المؤشر الشعاعي لكلكرين ولورنس (KL) مع قياس الوزن والطول, عرض الحيز الانسي للمفصل الظنبوبي, التغييرات الكيمياوية المختبرية المتمثلة بمستوى الكالسيوم, مستويات الدهون بانواعه متمثلة بقياس نسبة الكولسترول , الدهون الثلاثية, البروتينات الدهنيه عالية الكثافة والبروتينات الدهنية واطئة الكثافة , فوسفات الالكلاين والبروتين التفاعلي C وقياس المعلمات المناعية بطريقة الالايزا لكل من معلم البناء 1β TGF , معلم انحلال الغضروف CTXII, مستوى لبتين في الدم, مايلوبيروكسيدييز MPO. حيث تم قياس المستويات المذكوره اعلاه قبل البدا باخذ العلاج. تم اعطاء المرضى 10 ملغم من عقار الندرونيت الصوديوم (ALN) واعيد تقييم حالة المرضى بعد اكمال الاشهر الثلاثة من العلاج.النتائج : لوحظ ان هناك تحسن معتد على الفعاليات الفيزيائية واعراض الفصال العظمي , مع انخفاض معتد في معلم TGF β 1 (لاتوجد مقارنة بين تغيره وتحسن حالة المرضى, غير ان له علاقة بزيادة كتلة الجسم) , معلم انحلال الغضروف ) CTXIIحيث له علاقة بتحسن الفعاليات الحياتيه للمرضى), مستوى لبتين في الدم. مع تغير غير معتد في عرض الحيز الانسي للمفصل, مستوى الكالسيوم, مايلوبيروكسيدييز, مستويات الدهون بانواعه متمثلة بنسبة الكولسترول , الدهون الثلاثية, البروتينات الدهنيه عالية الكثافة والبروتينات الدهنية واطئة الكثافة , فوسفات الالكلاين والبروتين التفاعلي C. الاستنتاج : عقارالندرونيت صوديوم له فعالية كبيرة في تحسين الفعاليات الحياتية لمرضى الفصال العظمي وتقليل الالم الناتج من المرض دون تاثير معتد على تغير تركيب المفصل على الرغم من تقليله لمعلم اضمحلال الغضروف. عدم تاثير العقار على مستويات الدهون في الجسم المتمثلة بنسبة الكولسترول , الدهون الثلاثية, البروتينات الدهنية عالية الكثافة والبروتينات الدهنية واطئة الكثافة يجعله امنا نسبيا للمرضى المصابين باختلال نسبة الدهون. | Background : Osteoarthritis is a common arthritic disorder and responsible for 2 % of disability of people in all world. Osteoarthritis has believed to be principally a cartilage defect associated with focal articular cartilage degradation. Alendronate sodium (ALN) interferes with normal bone formation and turnover cycle in the body by slowing bone loss with elevating bone mass. . Aims of the study : This study was done to evaluate the effects of alendronate sodium on disease activity and physical function in patients with knee osteoarthritis, to evaluate the biochemical parameters in osteoarthritis patients including serum alkaline phosphate, calcium, c - reactive protein and lipid profile in form of analysis of serum cholesterol, triglyceride, low density lipoprotein and high density lipoprotein. Also to study the impact of ALN on anabolic in form analysis of serum transforming growth factor beta 1 (TGF - β1) , cartilage degradative in form of C - terminal cross linked - telopeptide of type II collagen (CTXII), Myloperoxidase (MPO) and leptin markers in osteoarthritis patients and to determine its effectiveness in slowing progression of disease. .Patients and methods : The patients were habitants of the cities of Al - Diwaniyhia (consulting the Rheumatologic Unit in Al - Diwanyhia Teaching Hospital) and Baghdad (consulting the Rheumatologic Unit in Baghdad Teaching Hospital). From those patients, 116 osteoarthritic patients 45 years old and more with Kellgren and Lawrence X - ray grade II and more with knee pain were enrolled in this study. Base line assessment was done including measurement of joint space width, assessment of WOMAC scoring(pain, function and stiffness), body mass index and the biochemical parameters (serum alkaline phosphate, calcium, c - reactive protein and lipid profile in form of analysis of serum cholesterol, triglyceride, low density lipoprotein and high density lipoprotein) with enzyme - linked immunosorbent assay (ELISA) analysis of serum (transforming growth factor beta 1 (TGF - β1) , C - terminal cross linked - telopeptide of type II collagen (CTXII), MPO and Leptin. They were instructed to take ALN 10 mg daily. Reassessment was done after 3 months of treatment. Results : A significant symptomatic improvement in WOMAC scoring regarding pain and stiffness (P<0.001) were observed associated with non significant improvement in function, significant reduction (P<0.001) in serum CTXII, Leptin and TGF - β1. A nonsignificant reduction in serum calcium and MPO, associated with no significant changes in serum Alkaline phosphatase, C - reactive protein, lipid profile(serum cholesterol, triglyceride, low density lipoprotein and high density lipoprotein) and joint space width were also reported. CTX II after treatment showed no significant correlation with BMI, however it showed significant positive correlations with WOMAC score. On the other hand, TGF - β1 showed significant positive correlation with BMI, but no significant correlations with WOMAC score. Conclusion : Alendronate sodium in patients with osteoarthritis has clinical efficacy in reducing symptoms especially pain through inhibition of leptin and CTXII with no significant structural improvement despite reduction of CTXII and TGF - β1 with no effects on inhibition of further disease progression. Relatively safe in old patients with dyslipidemia since there is no associated lipid disturbances with ALN. The reduction in level of CTX - II in the present study may be considered as one of the mode of actions of alendronate in patients with osteoarthritis

تقييم الطفرات الوراثية ل BRAF, IDH1 وفقدان 1p/19q في الاورام الدبقية للمرضى القراقيين : دراسة نسيجية مناعية كيميائية مع التهجين الموضعي المتفلور == Assessment of BRAF, IDH1 mutations and 1p/19q loss in Gliomas in Iraqi patients. Immunohistochemical and Fluorescence in Situ Hybridization study

اسم المؤلف: زهراء مروان شعبان العمر
اسم المشرف: سالم رشيد حمودي العبيدي
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الامراض - الوراثة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Background : - Gliomas account for almost 80% of primary malignant brain tumors, so, they considered the most common primary malignant brain tumors in adults. The major types of glial tumors are astrocytomas, oligodendrogliomas, and ependymomas. Isocitrate dehydrogenases 1, BRAFV600E and 1p/19q co - deletion are important molecular markers nowadays for diagnosis and prognosis of gliomas. Isocitrate dehydrogenase mutation might be encounter in the low - grade glioma, occurs in early stages of development and directs the progression of the tumor to a higher grade. BRAFV600E is a point mutation, resulting in a valine to glutamic acid substitution at position 600 and this mutation occurs more in pediatric gliomas but less frequent in adult gliomas. Co - deletion of 1p/19q results from a non - balanced translocation t (1 : 19) (q10 : p10) with subsequent loss of one of the derivative chromosomes and highly associated with oligodendroglial tumors morphology and improved survival.Aim of the study : - To assess the frequency of Isocitrate dehydrogenases 1 and BRAF mutations in Iraqi patients with gliomas by immunohistochemical study, to correlate their immunoreactivity with some clinicopathological parameters. To validate frequency of 1p/19q loss by Fluorescence in Situ Hybridization study and correlate the deletion with the some parameters.Patients and Methods : Formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in Baghdad city were collected in this retrospective selective study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that is manual tissue microarray, in which twenty - four small cores (each measure 2mm) of represented tissue made, then cut by microtome. IHC detection of Isocitrate dehydrogenases 1 and BRAFV600E antibodies did by Dako autostainer link 48. Assessment of co - deletion of 1p/19qAbstractIII

دراسة جزيئية وبكتريولوجية لبكتريا Bacteroides fragilis المعزولة من النساء المصابات بالتهاب المهبل البكتيري == Molecular and Bacteriological Study of Bacteroides fragilis Isolated from Women with Bacterial Vaginosis

اسم المؤلف: زهراء قيس سلمان
اسم المشرف: الهام عباس بنيان الساعدي | بشرى جابر عمران
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: شملت هذه الدراسة 150 مريضة يعانين من التهاب المهبل البكتيري ، تم جمع ما مجموعه 300 مسحة من عنق الرحم وافرازات مهبلية من هؤلاء المريضات بمعدل مسحتين اثنتين لكل مريضة و150 عينة دم جديدة تم جمعها من المريضات عند مراجعتهن الى مستشفيين في محافظة بابل هما : مستشفى بابل للنسائية والاطفال التعليمي ، ومستشفى الحلة التعليمي العام، و150 عينة دم جديدة كمجموعة سيطرة ، خلال الفترة من شباط الى تشرين الاول 2016. باستخام معايير امسلز تم تشخيص النساء اللواتي يعانين من التهاب المهبل البكتيري. وقد خضعت المسحات لطرق مختلفة لتحديد B.fragilis باستخدام الاساليب البكتريولوجية التقليدية. وجد ان 31 عزلة موجبة (20.6٪) باستخدام الاوساط الانتقائية. فضلا عن ذلك، تم تطبيق طرق الكشف الجزيئي باستخدام المعلم الوراثي المستند على دور جين 16 sRNA ، وكانت 44 عزلة (29.33٪) ايجابية وتعود لبكتريا B.fragilis وكذلك استخدم المعلم الوراثي nanH حيث كانت 39 عزلة (88.6٪) ايجابية. بالاضافة الى ذلك، تم فحص العزلات المعزولة على الاوساط الانتقائية الخاصة بالبكتريا للكشف عن قابلية البكتريا للالتصاق (adherence ability) بالخلايا المخاطية. كما تم التحري المظهري عن عملية تكوين الاغشية الحيوية (Biofilm formation) للعزلات المدروسة باستخدام فحص tissue culture plate (TCP) test اذ كانت مكونة للاغشية بنسبة (100%). حيث اظهرت العزلات 31 الموجبة المستخرجة من الوسط الانتقائي قدرة البكترياعلى تكوين ال Biofilm وكانت نسبة تكوين البيوفيلم (83.9٪) و(16.1٪) للوضع العالي والمعتدل على التوالي، في حين لم تكن هناك عزلات غير قادرة على تشكيل بيوفيلم. اما فيما يتعلق بتاثير بوفيدون - اليود بنسبة 10٪ على تكوين البيوفيلم، فقد اظهرت نتائج هذه الدراسة ان قدرة البكتريا على تكوينه قد تقلصت لتكون نسبة التشكيل قوي (6.5٪ (ومعتدل (71٪) و(22.6٪) لم يظهر اي تشكيل للبيوفيلم . تم فحص تاثير 10% povidone - iodine المستخدمة في الطب النسائي على انتاج الاغشية الحيوية . وقيمت الدراسة قابلية B.fragilis على انتاج bacteriocin وتاثيرها على بعض العزلات البكتيرية. اظهرت النتائج ان لعزلات البكتريا قدرة تازرية لتثبيط نمو العزلات المدروسة بنسب. فضلا عن ذلك، هدفت هذه الدراسة الى التعرف على علاقة التغايرالجيني(TLR - 4 Polymorphism) باستخدام تقنية البلمرة والقطع بالانزيم (PCR - RFLP) .وتبين من النتائج وجود ثلاث انماط جينية وكانت نسب انتشارها في مجموعة المرضى CC(36.0%) وThr399Ile : CT(12.0%) ,TT(52%) بينما نسب انتشارها في مجموعة السيطرة كانت TT(34.0%) , CC(60.0%) and CT(6.0%) وقد اظهرت وجود اختلاف وهو اختلافا معنويا, فيما بلغت نسبة انتشار الاليل T في المرضى (58%) بالمقارنة للسيطرة (37%) مع وجود اختلاف معنوي .وفي المقابل سجل وجود ثلاث انماط جينية في موقع طفرة Asp299Gly : AA(46.0%) ,GG(50.0%) and GA(4.0%) في مجموعة المريضات بينما في مجموعة السيطرة كانت نسب الانتشار AA(34.7%) , GG(59.3%) and GA(6.0%) مع عدم وجود فارق معنوي بينهما , اما نسبة انتشار الاليل G (52%) في مجموعة المريضات بالمقارنة بمجموعة السيطرة (62%) ويعد فارقا معنويا بينهما . تناولت الدراسة تحليل تسلسل جينات معينة من عزلات B.fragilis وكشفت نتائج التحليل للاحماض الامينية لجين Enterotoxin حيث تم تحديدها في 117/117 (100٪ (بمقارنة تسلسلها مع تلك التي في قاعدة البيانات في بنك الجينات وكانت نسبة الثغرات بينهما (0)٪ ، اما بالنسبة للعزلة الثانية فقد تم التعرف على نتائج التحليل للاحماض الامينية لجين Enterotoxin في 116/116 (100٪) وبثغرات 0/116 (0٪)، اما بالنسبة لجين Phospholipaseتم تحديدها في 129/129 (100٪) مع ثغرات 0/1129 (0٪) لكل من العزلتين . اخيرا تم تحليل العلاقات التطورية بين العزلات محلية من B.fragilis والعزلات العالمية من B.fragilis باستخدام تسلسل الجينات لل Enterotoxin وPhospholipaseحيث اظهر تحليل تسلسل الحمض النووي لجين Enterotoxin ان عزلات B.fragilis المحلية (NO.1) و(No.2) كانت مرتبطة ارتباطا وثيقا بعزلة B. fragilis المسجلة عالميا (AB026625.1) و(U67735.1) ونسبة التغير الجيني الكلي في شجرة التطور (0.01%) في حين ان نتائج تحليل النشوء والتطور من تسلسل جينات Phospholipase كشفت ان نفس العزلات المحلية (NO.1) و(No.2) كانت مرتبطة ارتباطا وثيقا بالعزلة المسجلة عالميا (CP011073.1) ، ونسبة التغير الجيني الكلي في شجرة التطور كانت (0.003 - 0.005%). | This study included 150 women with bacterial vaginosis (BV), a total of 300 swabs (as two high vaginal swabs for each woman and vaginal discharge ) were taken from each woman. In addition to fresh blood samples were collected from the same 150 women with bacterial vaginosis and 150 healthy women as control group , these women admitted to the out - patient clinics of Gynecology and Obstetrics , in two hospitals of Babylon Province : Babylon Maternity and Pediatrics Teaching Hospital, and Al - Hillah General Teaching Hospital during the period from February until October 2016. Women suffering from bacterial vaginosis were diagnosed according to Amsels criteria .The high vaginal swabs were subjected to different methods for identification of Bacteroides fragilis by using traditional bacteriological methods . It was found that 31(20.6%) isolates were recovered by using selective media . Furthermore , molecular detection methods was applied by using 16s rRNA gene ,where 44(29.33%) isolates were positive , and using nanH gene where 39(88.6%) isolates were positive out of the 44 isolates , these genetic markers were used for confirmation detection of B.fradilis isolates . In addition , the 31 isolates recovered from selective media were investigated to detect adherence of B.fragilis to mucosal epithelia cells .Also , biofilm formation was tested in semi quantitative microtiter plate test . The result revealed that all isolates were biofilm former , biofilm formation were accounted (83.9%) and (16.1%) for high and moderate mode respectively , while there were no isolates that do not express biofilm formation . Regarding on effect of 10% povidone - iodine on biofilm formation ,the result of this study demonstrated that internal douche effectively reduced the viability of biofilm forming to strong (6.5%) , moderate (71%) and (22.6%) shown no biofilm formation . The study was also evaluated the susceptibility of bacteriocin produced by B.fragilis on some isolates of bacteria . The result revealed that B.fragilis have synergistic ability to inhibition the growth of studied isolates with different ratios . Additionally, this study aimed to investigate the relation of Asp299Gly and Thr399Ile Single Nucleotide Polymorphism (SNPs) in the Toll - like receptor - 4 (TLR - 4) with the incidence of bacterial vaginosis .The result elucidated that at the site of Thr399Ile SNP, there were three genotypes for this SNP among patients ; TT,CC and TC with frequency of (52%) ,(36.0%) and (12.0%) respectively , whereas , the genotype frequency among control group were TT(34%) ,CC(60%) and TC(6.0%) with significance differences between patients and control . However, the frequency of allele T (mutant) was higher among patients (58%) than control (37%) with significant difference. The site of Asp299Gly SNP also showed three genotype among patients with frequency of AA(46.0%), GG(50.0%) and GA(4.0%) , whereas the genotype frequency among control group were AA(34.7%) , GG(59.3%) and GA(6.0%) with no significant differences between patients and control . Additionally , the frequency of allele G(mutant) was higher among control(62%) than patients (52%) with significant differences between them . The study deal with sequence analysis of specific genes of B.fragilis isolates revealed that the analysis results of amino acids for enterotoxin gene was identified in 117/117(100%) by comparing its sequence with that in the database in gene bank by Blast program with gaps 0/117(0%) and for the second isolate the analysis results of amino acids for enterotoxin gene was identified in 116/116(100%) and with gaps 0/116(0%) , while for phospholipase gene was identified in 129/129(100%) with gaps 0/1129(0%) for first isolates.Finally ,the phylogenetic relationships between two local isolates of Bacteroides fragilis and Bacteroides fragilis global isolates were analyzed using the enterotoxin and phospholipase genes sequence. The DNA sequencing analysis of enterotoxin gene revealed that B. fragilis local number (No.1and No.2) isolates were closely related to isolation B. fragilis recorded globally NCBI - BLAST B. fragilis (AB026625.1) and (U67735.1) , whereas other NCBI - Blast B.fragilis enterotoxin gene were show different out of tree at total genetic change (0.01%) , while the phylogenetic analysis results of phospholipase gene sequences revealed that B. fragilis local isolates ( No.1and No.2) were closely related to isolation B. fragilis recorded globally NCBI - BLAST B. fragilis (CP011073.1) , whereas other NCBI - Blast B.fragilis phospholipase gene showed difference out of tree at total genetic change (0.003 - 0.005 %).

الكشف الجزيئي للفايروس البشري المتوجه للخلايا اللمفاوية التائيه - النوع الاول والتعبير البروتيني ل P27, CDK6,CDK4 وBcl - 2 في سرطان الغدد اللمفاوية الهودجكن واللاهودجكن == Molecular Detection of Human T Lymphotropic Virus Type - 1 (HTLV - 1) and Expressional Proteins of CDK4, CDK6, P27 and Bcl - 2 in Hodgkin?s and Non - Hodgkin?s Lymphomas

اسم المؤلف: نور سامي عبود الليباوي
اسم المشرف: جواد كاظم طراد الخفاجي | شاكر حماد محمد العلواني
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: صممت هذه الدراسة على انها دراسة بحثية ذات اثر رجعي ,وسيطره . اذ اشتملت على120خزعة نسجية من منطقة الغدد اللمفاوية المحفوظة بالفورمالين والمطمورة بشمع البارافين.جمعت هذه النماذج من ارشيفات الانسجة المرضية لمختبرات مستشفى الحلة التعليمي/ محافظة بابل , مستشفى الحسين التعليمي/ محافظة كربلاء ,مستشفى الصدر التعليمي/ محافظة النجف ,مستشفى الحسين التعليمي/ محافظة المثنى ومستشفى القادسية التعليمي/ محافظة القادسية وكذلك العديد من مختبرات الانسجة المرضية الخاصة الموجودة في بابل ,بغداد, كربلاء, القادسيه, المثنى اذ جمعت هذه العينات للفترة من اذار 2016 الى كانون الاول 2016. هذه النماذج تعود للمرضى المصابين بسرطان العقد اللمفاوية للسنوات من 2011 لغاية 2016 .قسمت هذه العينات حسب المجموعات التالية : - 1 - اربعون قطعة نسيجية لمفاوية مصابة بسرطان العقد اللمفاوية نوع هودجكن .2 - اربعون قطعة نسيجية لمفاوية مصابة بسرطان العقد اللمفاوية نوع اللاهودجكن .3 - اربعون قطعة نسجية لعقد لمفاوية (لايظهر عليها اي تغيرات مرضية نسيجية) ظاهريا سليمة استعملت كمجموعة سيطرة .بعد التقطيع النسيجي لخزع العقد اللمفاوبة وتصبيغها باستخدام صبغتي الايوسين والهيماتوكسلين تم اجراء الفحص التاكيدي الدقيق لغرض التشخيص النهائي من قبل اخصائي الامراض النسيجية لهذه الدراسة. تم تصميم الجانب العملي لهذه الدراسة الذي اتخذ مسارين رئيسين : - 1. التحديد الجزيئي للفايروس البشري المتوجه للخلايا اللمفاوية التائيه من النوع الاول بفحصها داخل خلايا الانسجة اللمفاوية حيث تم انجازه بوساطة استخدام تقنية التهجين الموضعي ذات الحساسية العالية .2. اجراء دراسة كيميائية - نسيجية مناعية لوصف الحالة التعبيرية ل CDK4 وCDK6 ومثبط CDK (p27) وفحص الجينات السرطانية للخلايا اللمفاوية B (Bcl - 2 ) في الانسجة المصابة بالهودجكن واللاهودجكن والعقد اللمفاوية السليمة ظاهريا (غير مصابة).ان مجمل النتائج التي تم الحصول عليها هذه الدراسة كانت كما يلي : 1 - نسبة الذكور( (57.5% المصابين بسرطان الغدد اللمفاوية الهودجكن واللاهودجكن ومجموعة السيطرة كانت اعلى من نسبة الاناث (42.5%) في هذه المجاميع الثلاثة. حيث كانت نسبة الذكور/اناث هي1 : 1.35.2 - اظهر الفحص النسيجي ان النسبة العليا من مرضى الهودجكن كان من الدرجة المنخفضة (42.5%) يتبعها الدرجة المتوسطة (35%) وادنى نسبة هي الدرجة العليا (22.5%). بينما النسبة العليا من مرضى اللاهودجكن كانت من الدرجة المنخفضة والمتوسطة %37.5)) وادنى نسبة هي الدرجة العليا ( 25%).ا - نتائج الفايروس البشري المتوجه للخلايا اللمفاوية التائيه من النوع الاول وCDK4 وCDK6 ومثبط CDK (p27 ) وكذلك الجينات السرطانية للخلايا المفاوية B (Bcl - 2 ) في الانسجة المصابة بالهودجكن : 1 - النتائج الموجبة لجين HBZ للفايروس البشري المتوجه للخلايا اللمفاوية التائيه من النوع الاول بتفاعل التهجين الموضعي كانت ( 22.5%) 2 - اظهرالاختبارالكيميائي النسجي المناعي لـ p27 ان 35% من الانسجة كانت موجبة .3 - اظهرالاختبارالكيميائي النسجي المناعي لـ CDK4 ان 35% من الانسجة كانت موجبة .4 - اظهرالاختبارالكيميائي النسجي المناعي لـ CDK6 ان 57.5 % من الانسجة كانت موجبة . 5 - اظهرالاختبارالكيميائي النسجي المناعي لـ Bcl - 2ان47.5 % من الانسجة كانت موجبة .6 - تم تمييز علاقة طردية قوية تربط بين تحديد والانتشارالتعبيرالبروتيني والكثافة للفيروس وCDK4 .7 - وجدت علاقة طردية قوية بين تحديد والانتشارالتعبيرالبروتيني والكثافة للCDK4 وCDK6 .وايضا وجدت علاقة بين تحديد وكثافة ال CDK6 وBcl - 2.8 - تم تمييز علاقة طردية قوية تربط بين تحديد والانتشارالتعبيرالبروتيني والكثافة ل p27 وعمر المرضى. كذلك علاقة طردية بين p27 وBcl - 2.ب‌ - نتائج للفايروس البشري المتوجه للخلايا اللمفاوية التائية من النوع الاول وCDK4 وCDK6 ومثبط CDK (p27 ) وكذلك الجينات السرطانية للخلايا المفاوية B (Bcl - 2 ) في الانسجة المصابة باللاهودجكن : 1 - النتائج الموجبة لجين HBZ للفايروس البشري المتوجه للخلايا اللمفاوية التائيه من النوع الاول بتفاعل التهجين الموضعي كانت (45%) بينما لم تظهر اي حالة موجبة بتفاعل التهجين الموضعي للفايروس ضمن مجموعة السيطرة .2 - اظهرالاختبارالكيميائي النسجي المناعي لـ p27 ان 42.5% من الانسجة كانت موجبة. بينما لم تظهر اي حالة موجبة للp27 بواسطة الاختبارالكيميائي النسجي المناعي ضمن مجموعة السيطرة.3 - اظهرالاختبارالكيميائي النسجي المناعي لـCDK4 ان 50% من الانسجة كانت موجبة. بينما لم تظهر اي حالة موجبة في هذا الاختبار في مجموعة السيطرة.4 - اظهرالاختبارالكيميائي النسجي المناعي لـ CDK6 ان 72.5% من الانسجة كانت موجبة . بينما لم تظهر اي حالة موجبة في هذا الاختبار في مجموعة السيطرة.5 - اظهرالاختبارالكيميائي النسجي المناعي ل Bcl - 2 ان 72.5% من الانسجة كانت موجبة. بينما لم تظهر اي حالة موجبة في هذا الاختبار في مجموعة السطرة.6 - تم تمييز علاقة طردية قوية تربط بين تحديد الفيروس وBcl - 2 وكذلك وجدت علاقة قوية طردية تربط بين الانتشارالتعبيرالبروتيني والكثافة للفايروس وCDK6.7 - وجدت علاقة طردية قوية بين تحديد والانتشارالتعبيرالبروتيني والكثافة للBcl - 2 وCDK6 .وايضا وجدت علاقة بين تحديد وكثافة ال p27 وBcl - 2 فقط .كذلك وجدت علاقة عكسية بين عمر المريض وBcl - 2 .8 - تم تمييز علاقة طردية تربط بين كثافة CDK4 وp27 وكذلك بين CDK4 وBcl - 2

دراسة بكتيرية ووراثية لبكتريا Gardnerella vaginalis وعلاقتها بالولادة المبكرة == Bacteriological and Molecular study of Gardnerella vaginalis association with preterm labor

اسم المؤلف: ازل علاء عباس الربيعي
اسم المشرف: الهام عباس بنيان الساعدي | لميس عبد الرزاق عبد اللطيف
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: In this study , one hundred fifty samples were collected from paitents with preterm labor (PTL), who have been attending to Babylon Maternity and pediatric hospital and Al - Hilla Teaching Hospital, at the period from February to October 2016. Two swabs were collected one for culturing and the other for direct extraction for isolation Gardnerella vaginalis.Out of the 150 samples only 6(4%) on culture and 30 (20%) on molecular level isolated from preterm labor caused by Bacterial vaginosis , Urinary tract infection and aborted women. The results isolation and laboratory diagnosis as well as biochemical test approved that there is only 6 isolates belong to G.vaginalis confirmed by using Vitek 2 system and molecular detection by specific primers.Preterm birth is one of the most common causes of neonatal morbidity and mortality. Associated with sub sequent preterm labor in up to 40% of cases as shown in our resultsThe isolates are varied in their ability to produce sidrophore and extracellular protease in addition to their hemolytic activity .Adherence activity were investigated and indicate that G.vaginals have pili on her surface help her to attached and initiated infection Metronidazole was effective in prevent adherence at low concentration (5%) was 100% percentage.All isolates are able to produce biofilm in different level which provided as main step in pathogenesis caused it can evade and evoke the action of immune response and antibiotics , Alum , sodium chloride and sodium bicarbonate were shown very high effectively to eradicationSummaryIIG.vaginalis biofilm which gave results (0%) whereas the thymol extract (carvacrol) were moderate in action gave (50%) effect on Biofilm .Results also preveld that increased of pH value give a chance to overgrowth of G.vaginalis .The results indicated that Lactic acid considered as a limiting factors preventing growth of G.vaginalis when its grown with lactic acid , measured by optical Density (OD) the high production level is (1.901) in 2% concentration Whereas the lowest level is (0.32) in 10% concentration.Isolates Have ability to produce wide spectrum Bacteriocin which inhibited growth of both Gram positive and Gram negative bacteria that founded in vagina environment, Staphylococcus saprophyticus were the most bacteria that inhibited .extracted Vaginolysin was have a completed hemolytic activity in different temperatures (37 - 38 - 40 - 42). The results showed that its activity as were increased in high temperature.Recent study were clarified that purified vaginolysin was active in presence of cholesterol in media, gave (1.205) growth rate measured by the optical density at (100%) concentration. vaginolysin is cholesterol - dependent cytolysin (CDCs) toxin.Antimicrobial activity were investigated to the extracted Vaginolysin to evaluation of its activity depending on inhibition zone, the results showed that candida is more affective between the other microorganism gave (1.3)mm.Some virulence factor have been detected in molecular level ( Vly - SI - LuxS - LexA - rec a - Pl - Fur - Omp - Gal - protease ). Which wereSummaryIIIvaried in production may be as a results to the un presence of this gene in bacteria , may be silent gene or due to the amount of bacteria in sample are rare.Three Antibiotics were used at a molecular level (Tet b) were varied between resistance and sensitive to this gene (10) sample are resist,(RdxA) all sample are sensitive to its gene at percentage (100%), whereas (erna)gene were resist in all samples (100%).The results show there was no sequence homology between Mycoplasma spp. and G. vaginalis in the adhesion level this were detected by P30/P32 specific primer .The outcome of RFLPS - PCR is confirmed that G.vaginalis is virulent and have a role in preterm labor by degradation of membranes with the association of variable Silidase and presence of β - Galactosidase .MBL (mannose binding lectin ) levels were have a direct role pregnancy. It is also apparent that MBL plays a role in the defense against vaginal infection. MBL is essential to successful pregnancy in regards to maternal defense against infection, recent study indicate that MBL deficiency related to pregnancy outcome disorderedthis considered the first study of mannose in Iraq .DNA sequencing were done for two main virulence factor Phospholipase gene and Quorum sensing gene which showed some variation , then recorded in NCBI - gene sequencing as first descriped in Iraq.

التاثير المايكروبي المضاد لدقائق الفضة النانوية ضد بكتريا الزوائف الزنجارية المعزولة من عينات سريرية == The Antimicrobial Effect of Silver Nano Particles (AgNPs) Against Pseudomonas aeruginosa Isolated from Clinical Samples

اسم المؤلف: سارة عقيل حسن التميمي
اسم المشرف: هدى هادي الحسناوي
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة للكشف عن التاثيرالمايكروبي المضاد لدقائق الفضة النانوية ضد بكتريا الزوائف الزنجارية المعزولة من عينات سريرية, تم جمع 100 عينة من مصادر سريرية مختلفة, تضمنت العينات السريرية عينات الحروق , والادرار, والدم, واصابات الاذن, والقشع تم اخذ هذه العينات من المرضى الراقدين في مستشفى الحلة التعليمي خلال الفترة من تشرين الثاني 2016 الى كانون الثاني 2017. زرعت جميع العينات على وسط السترمايد, وسط اكار الدم, وحضنت كل الاطباق هوائيا بدرجة حرارة C°37 لمدة 18ساعة. تم تشخيص بكتريا الزوائف الزنجارية بالاعتماد على الصفات المظهرية والكيموحيوية.كان توزيع العزلات كالاتي : 22(52.4%) من عينات الحروق, 8(19%) من عينات الادرار, 14.3)6%) عينة من المرضى المصابين بالالتهاب الاذن الخارجية, 4(9.5%) من عينات الدم,2(4.7%) من عينات القشع. اظهرت نتائج اختبار الحساسية باستخدام طريقة الانتشار في الوسط ان جميع العزلات(26) كانت مقاومة لكل من الببراسلين (100%), السيفوتاكسيم (100%),بينما نسبة المقاومة لكل من الاميكاسين (88%), سيفترياكسون (65%), جنتامايسن (62%), بينما وجدت نسبة مقاومة قليلة للسفتازديم (15%), وكل العزلات كانت حساسة (100) لكل من الميروبينيم, اوفلوكساسين, نورفلوكساسين والسبروفلوكساسين. تم في هذه الدراسة ايضا الكشف عن تكوين الاغشية الحيوية بواسطة طريقتين : طريقة الطبق الزرعي النسيجي (TCP) وطريقة صبغة الكونكو الحمراء (CRA) فكانت النتائج كالاتي : وجد ان في طريقة الطبق الزرعي النسيجي كانت جميع عزلات الزوائف الزنجارية والبالغ عددها (9) منتجة للاغشية الحيوية, (4) من اصل (9) (44%) من عزلات الزوائف الزنجارية انتجت مستعمرات سوداء بواسطة طريقة صبغة الكونكو الحمراء. وكذالك في هذه الدراسة تم الكشف عن تاثير جزيئات الفضة النانوية ((AgNPs على تكوين الاغشية الحيوية باستخدام تراكيز مختلفة (µg/ml 2.5, µg/ml 5, 10 µg/ml, 20µg/ml),حيث اظهرت النتائج ان اعلى فعالية لجزيئات الفضة النانوية كانت عند التركيز (20) وبنسبة تثبيط (100%), من جهة اخرى تم اختبار التاثير البكتيري المضاد لجزيئات الفضة النانوية ضد مختلف انواع العزلات البكتيرية باستخدام طريق التخافيف في الوسط الصلب وتراكيز مختلفة من جزيئات الفضة النانوية (12.5 µg/ml, 25 µg/ml, 50 µg/ml, (100 µg/ml, 200 µg/ml وكانت العزلات كالتالي : ـ(S. aureus, E. coli, E. cloacae, K. pneumonia, P. aeuroginosa)اظهرت النتائج ان اعلى فعالية لجزيئات الفضة النانوية كانت عند التراكيز(100 µg/ml, 200 µg/ml).ايضا تم تعريض ظاهرة السوارمنك في عزلات الزوائف الزنجارية لتراكيز مختلفة من جزيئات الفضة النانوية (2.5 µg/ml, 5 µg/ml, 10 µg/ml ), ووجد ان اعلى نشاط لجزيئات الفضة النانوية ضد ضاهرة السوارمنك وانتاج البايوسيانين كانت بتركيز ((10 µg/ml, 5 µg/ml .تم زراعة جميع عزلات بكتريا الزوائف الزنجارية (26)عزلة بوجود نترات الفضة (AgNO3) بتراكيز تتراوح (100 µg/ml - 400 µg/ml ) باستخدام طريقة التخافيف في الوسط الصلب مع قدرات مختلفة لانتاج البيوسيانين. اظهرت النتائج ان جميع العزلات 26((100 المنتجة للبايوسيانين كانت مقاومة للسلفر نايتريت (AgNO3) من خلال ظهور نمو في الطبق الحاوي على التراكيز المذكورة نترات الفضة (AgNO3).في هذه الدراسة تم اختبار بكتريا الزوائف الزنجارية لانتاج البكتريوسين باستخدام عزلات بكترية مختلفة تضمنت العزلات (امعائية مذرقية,مكورات العنقودية الذهبية,اشريكية قولونية, كلبسيلة رئوية). كذلك تم العثور على مجموعة واسعة من منطقة تثبيط (24mm - 30mm) في الانواع البكتيرية اعلاه مع قطر (30mm, 28mm, 24mm, 30mm) على التوالي. عزلات (امعائية مذرقية, كلبسيلة رئوية) كانت الاكثر تاثر والتي انتجت قطر تثبيط ((30mm. في هذه الدراسة ايضا تم تقييم علاقة مقاومة الفضة مع انتاج بيوسيانين في بكتريا الزوائف الزنجارية متعددة المقاومة للمضادات الحيوية. في هذه الدراسة تم استخدام تقنية تفاعل البلمرة المتسلسل للتاكد من وجود بعض الجينات. في هذه التقنية، تم فحص جينات bla - IMP)) التي تشفر الى انزيم ميتالو - β - لاكتاميز المسؤول عن مقاومة ايميبينيم وجينات (silS ,silE) التي تشفر لمقاومة الفضة في عزلات بكتريا الزوائف الزنجارية بواسطة استخدام برايمرت خاصة. وقد وجد ان جميع العزلات ال 26 (100%) من بكتريا الزوائف الزنجارية كانت سلبية لهذه الجينات. كما تم استخدام اثنين من البرايمرات المحددة للكشف عن فينازين (بيوسيانين) (phzS, phzM). اظهرت النتائج لل (phzM) ان من اصل 26)) عزلة , 21 (80.8%) اعطت نتيجة ايجابية, , فيما يخص (phzS gene) وجد ان 14(% 53.9) عزلة كانت حامله لهذا الجين . والخاتمة ان جزيئات الفضة النانوية (AgNps) تمتلك تاثير ضد الاغشية الحيوية وايضا لها تاثير بكتيري مضاد ضد بكتريا الزوائف الزنجارية وانواع بكتيرية اخرى, والتي من الممكن استخدامها كعلاج بديل. فيما يخص انتاج البايوسيانين ومقاومة الفضة، ظاهريا كان هناك تاثير البايوسيانين على مقاومة الفضة ولم تكن مقاومة الفضة بسبب نقل وجود جينات (silS, silE). | This study was conducted to detect the antimicrobial effect of silver nanoparticles (AgNPs) against Pseudomonas aeruginosa isolated from clinical samples, total of 100 specimens were collected from different clinical sites included urine, burns, ear, blood , and sputum. The specimens were taken from patients admitted to Al - Hilla Teaching Hospital during a period extended from November 2016 to January 2017. All specimens were cultured on selective medium (cetrimide agar), blood agar, then incubated at 37°C for 18 hr. The isolated of P. aeurginosa were diagnosed depending on morphological and biochemical characteristics.The distribution of P. aeurginosa isolates was as follows : 22(52.4%) isolates from burns, 8(19%) isolates from urine, 6(14.3%) isolates from patients with otitis externa, 4(9.5%) isolates were isolated from blood and 2(4.7%) isolates from sputum. Antibiotic susceptibility test using disc diffusion test revealed that all isolates (26) were resistant (100%) to Piperacillin, Cefotaxime. While the resistance rate for Amikacin (88%), Ceftriaxone (65%), Gentamicin (62%), but low resistance rate was found to Ceftazidime (15%) and all isolates were sensitive (100%) to Meropenem, Ofloxacin, Norfloxacin and Ciprofloxacin. The biofilm formation in P. aeurginosa isolates was investigated by two methods include, tissue culture plates method (TCP) and congo red agar method (CRA). The results showed that all isolates 9 (100%) were found to be biofilm producer when investigated by TCP assay, and 4 out of 9 (44. %) isolates of P. aeurginosa were producing black colonies when detected by CRA method.The effect of AgNps on biofilm formation was tested by using different concentrations of silver nanoparticles (2.5 μg/ml, 5 μg/ml,10 μg/ml, 20μg/ml). The results showed that the highest activity was at a concentration of 20 μg/ml, with an inhibition rate of 100%. On the other hand the antibacterial activity of AgNps was tested against different bacterial species isolates (Staphylococcus aureus, Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Pseudomnas aeurginosa) using agar dilution method with different concentrations of silver nanoparticles (12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 200 μg/ml). The results showed that the highest activity was at a concentration of (100 μg/ml and 200μg/ml). On the other hand Swarming motility in P. aeruginosa isolates was detected and subjected to different concentrations of (AgNps) (2.5 µg/ml, 5 µg/ml, 10 µg/ml), it was found that the highest activity of AgNps against swarming motility and pyocyanin production of P. aeurginosa was at a concentration of (5 µg/ml, and 10μg/ml).The relation of silver resistance with pyocyanin production in P. aeruginosa was assessed. Screening test for silver resistance was performed by agar dilution method, all (26 isolates) of P. aeruginosa were cultivated in the presence of AgNo3 at a concentration (100 µg/ml - 200 µg/ml, 400µg/ml) using agar dilution method with differing capacities for pyocyanin production. All isolates of P. aeurginosa 26(100%) that produce pyocyanin were found to be resistant to AgNo3.In this study P. aeruginosa isolates were tested for bacteriocin activity by the agar well diffusion method using different bacterial isolates : E. coli, K. pneumoniae, S. aureus, E. cloacae. Wide range of inhibition zone (24mm - 30mm) was found against above bacterial species with diameter (28mm, 30mm, 24mm, 30mm), respectively. The most affected bacteria were K. pneumoniae and E. cloacae isolates, which produce inhibition zone (30)mm.PCR technique was used in this study for investigation some genes. In this technique, blaIMP gene that encode Metallo - β - lactamase enzyme responsible for imipenem resistance and (silE and silS) genes that encoding silver resistance were investigated in P. aeuroginosa isolates by using specific primers. It was found that all 26 (100%) isolates of P. aeuroginosa were negative for these genes. Also two specific primers were used to detect phenazine (pyocyanin) modifying genes (phzM, phzS). According to phzM gene, it was found that out of 26 P. aeuroginosa isolates, 21 (80.8%) isolates gave positive results at 330 bp, in regard to phzS gene, it was found out of 26 P. aeurginosa isolates, 14 (53.9%) isolates gave positive result at 664 bp.In conclusion AgNps have an antibiofilm, antibacterial activity against P. aeruginosa and different bacterial species that could be used as an alternative therapy. In regard to pyocyanin production and silver resistance, phenotypically there was effect of pyocyanin pigment on silver resistance and the silver resistance was not due to carriage of silE, silS genes.

التشخيص والتوصيف الجزيئي لبكتريا Clostridium perfringens المعزوله من حالات مرضية سريرية في مدينة الحلة == Molecular Diagnosis and Characterization of Clostridium Perfringens from Clinical Samples in Al - Hilla city

اسم المؤلف: علياء محمد حمود الشمري
اسم المشرف: علاء هاني الجراخ | ميساء صالح الشكري
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تهدف هذه الدراسة لمعرفة وبائية بكترياC. Perfringens في العينات المؤخوذة في محافظة بابل. تم جمع 140عينة من الجروح العميقة من المرضى الداخلين في مستشفيات مدينة الحلة الرئيسية (مستشفى الحله التعليمي ومدينة مرجان الطبيه) والذين يعانون من مرض السكري المزمن وايضا من مرضى تعرضوا الى طلق ناري فضلا عن المرضى الذين يعانون من التهاب الخلوي اللاهوائي في الجلد(anaerobic cellulitis ) للفترة من شهر شباط ولغاية شهرتشرين الاول 2016. بالاضافه الى ذلك تم تشخيص وعزل بكترياC. perfringens بطرق مختلفة منها استخدام الطرق البكتيرية التقليدية من خلال طرق التنمية على الاوساط الزرعية الاختيارية لهذه البكتريا واظهرت النتائج الحصول على 3 (2.14%)عزلات كان عزل اثنان منها 1.42%)) من مرضى السكري المزمن اما العزله الثالثه (0.72%) فقد تم الحصول عليها من الذين تعرضوا لطلق ناري في حين لم يتم الحصول على عزلات من المرضى الذين يعانون من الالتهاب الخلوي اللاهوائي . وكذلك تم تاكيد التشخيص باستخدام المعلم الوراثي المستند على دور جين 16s rRNA وجين16S - 23S spacer rRNA حيث اظهرت النتائج, من اصل 140 عزلة فان 7 عزلات تعود لبكتريا C. perfringens. بالاضافة الى ذلك, تم التحري عن قابلية هذه البكتريا واختبار قابليتها على انتاج انزيم (phpspholipase C) ووجد ان جميع العزلات منتجة الى هذا الانزيم. كما تم الكشف عن حساسية عزلات البكتريا اللاهوائية تجاه 24 مضادا حيويا. بينت نتائج الدراسة ان جميع العزلات اظهرت حساسية عالية (100%) تجاه كل من penicillins .مثل benzypencillin, ,amoxicillin فضلا عن حساسيتها تجاه  - lactamase inhibitor مثل Ampicillin - sulbactam وحساسيه عالية لكل من metronidazole وaminoglycosidesومن ناحية اخرى، اظهرت جميع العزلات مقاومة عالية لكل من مضادات tetracyclin, Linezolid, levofloxacin, erthromycin بينما اظهرت هذه العزلات تغاير في درجات المقاومة تجاه مضادات pipracillin, Clindamycin, and Impenem.تم استخدام الطرق الوراثية في الكشف عن جينات المقاومة للمضادات الحياتية كجيناتM,Q,W,B) ) tet الخاصة بالكشف عن المقاومة للتتراسايكلين وجينات erm(A,B) للكشف عن المقاومة للكلندامايسين وجين nim للكشف عن المقاومة للمترونيدازول حيث اظهرت النتائج ان الجين tet M كان ذا سيادة عالية بنسبة 71.42 %تم استخدام طريقة التشخيص الجزيئي لتوكسينات البكتريا (alpha,epsilon, iota) للاستدلال على وجود جينات (cpa, etx, iap)، في C. perfringens وبنسبة 100%, 28.4%, 28.4% )) على التوالي.بالاضافة الى ذلك تم استخدام جينات cpa, etx and iap لقياس تعاقب القواعد النيتروجينية للمادة الوراثية للعزلات المدروسة فضلا عن تسجيلها في بنك الجينات العالمي ( ,(Gene Bank,اذ تم خلال هذه الدراسة ولاول مرة في العراق، الحصول على خمسة ارقام انضمام الى بنك الجينات العالمي (5 accession numbers)، حيث تم نشر هذه الارقام في بنك الجينات الامريكي gene bank - NCBI وهذه الارقام هي : جينiap : KY523199, KY523200 اما فيما يخص الجينetx فكانت الارقام هي : KY523201, KY523202 وفيما يخص الجين cpaفكان رقم الانضمام هو : . KY523203تعد هذه الدراسه انها اول دراسه في العراق تضمنت تحليل , دراسه تعاقب القواعد النايتروجينية للمادة الوراثية فضلا عن التسجيل في بنك الجينات العالمي لسموم عزلات محلية من بكتريا C.perfringens معزولة من عينات سريرية. | This cross sectional and hospital - based study aimed to studying the incidenceof Clostridium perfringens in clinical samples in Babylon province. A total of 140 wound swabs were taken from patients admitted to two main hospitals in Hilla city, Iraq (Hilla Teaching Hospital and Medical Marjan City), during the period from February to October 2016. Swabs were collected from diabetic patients, anaerobic cellulitis, and bullet wounds. These wound swab samples were subjected to different methods for identification of C. perfringens according to standard method. It was found that 3 (2.14%) C. perfringens isolates were recovered, of which 2 isolates (1.42%) were obtained from diabetic patients, 1 isolates (0.72 %) from bullet wounds, No isolates are recovered from the other clinical samples (cellulitis). Furthermore, molecular detection method was applied by using 16s rRNA, 16S - 23S intergenic spacer rRNA genes as a genetic marker for confirmation of detection of C. perfringens isolates,7 isolates of C. perfringens out of 140 are detected by using PCR with specific primer.The study also focuses on the ability of this bacteria to produce Phospholipase C and it was found that all isolates are positive for this enzyme (100%) . The results of antibiotic susceptibility testing using Vitek 2 system and disc diffusion method (DDT) of C. perfringens isolates (No.= 3) against 24 antibiotics showed that all isolates were highly sensitive to penicillins, (benzylpenicillin, and amoxicillin), all isolates (3/3) were sensitive to  - lactamase inhibitor such as Ampicillin - sulbactum. They were more susceptible to metronidazole and aminoglycosides in (3/3). On the other hand, all isolates were highly resistant to tetracycline, Linezolid ,levofloxacin, and erythromycin However, these isolates expressed different degree of susceptibility towards other antibiotics (Clindamycin, piperacillin and Imipenem). Molecular detection of C. perfringens of antibiotic resistance genes had been investigated and it had been found that tetracycline resistance gene tet M is the most commonly observed (71.42%) among ribosomal protection genes among C. perfringens isolates. While all isolates (7) gave negative results for other tetracycline resistance genes (tet W and Q, tet B, erm(A), erm(B),nim gens).Furthermore, detection of clostridial toxin (alpha, epsilon, iota toxins was applied using specific genes (cpa, etx, iap) and the results showed that the rate of the presence of these genes are : 100%, 28.8% and 28.4% respectively. The following genes : cpa, etx and iap were used for sequencing analysis, registration in gene bank - NCBI. Five accession numbers were obtained from registration of five sequences of these three genes at gene bank - NCBI : iap gene; accession numbers are : KY523199, KY523200etx gene; accession numbers are : KY523201, KY523202cpa gene; accession number is : KY523203. This is the first study in Iraq which employed analysis sequencing and registration in gene bank - NCBI, of clostriaial toxin of local isolates of C. perfringens obtained from clinical samples

دراسة فيروس نظير الانفلونزا وبكتريا المسبحيات القيحية مع التهاب الاذن الوسطى القيحي في الاطفال == Study of Parainfluenza virus and Streptococcus pyogenes with suppurative otitis media in children

اسم المؤلف: ايام محمد صالح علي العامود
اسم المشرف: جواد كاظم طراد الخفاجي | غانم عبود المولى
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: هدفت هذه الدراسة الى التحري عن دور فيروس نظير الانفلونزا وبكتريا المسبحيات القيحية Streptococcus pyogenes في الاطفال الذين يعانون من التهاب الاذن الوسطى القيحي . جمعت 200 عينة (ثلاث مسحات لكل مريض تضمنت مسحة من افرازات الاذن ومسحة من افرازات الانف لدراسة الفيروس ومسحة من افرازات الاذن لدراسة البكتريا و50 عينة دم) من 50 مريض يعانون من التهاب الاذن الوسطى القيحي احيلوا الى مستشفى الحلة التعليمي في محافظة بابل للفترة من شباط (2016) لغاية ايار (2016). والذين يعانون من التهاب الاذن الوسطى. وتضمنت الدراسة 32 عينة ماخوذه من اشخاص اصحاء كمجموعة سيطرة وتم تقسيمهم الى ست مجاميع عمرية بنفس طريقة تقسيم مجاميع المرضى. شملت الدراسة اربعة اجزاء رئيسية : اولا التشخيص الفيروسي اعتمد على التقنيات المتقدمة (العزل الاولي باستخدام فحص التالق المناعي المباشر لتحديد وتمييز الانواع الثلاثة لفيروس نظير الانفلونزا 1,2, and 3 والتشخيص الجزيئي بواسطة استخدام فحص تفاعل سلسلة انزيم البلمرة بالوقت الحقيقي لتحديد وتمييز الانواع الاربعة للفيروس1,2,3, and 4 وتم استخدام المجهر الالكتروني النفاذ لمشاهدة جزيئة الفيروس . والجزء الثاني فحص البكتريا باستخدام فحوصات الكيموحيوية وفحص Vitek 2 system وتم التحري ايضا عن انزيم الهيمولايسين حيث كانت جميع العزلات منتجه لهذا الانزيم . التشخيص الجزيئي للكشف عن وجود الجين mga بواسطة تقنية سلسلة تفاعل انزيم البلمرة .الجزء الثالث شمل وجود الفيروس والبكتريا في عينات الاذن للمرضى المصابين بالتهاب الاذن الوسطى . الجزء الرابع تضمن دراسة مناعية لمعرفة دور الحركيات الخلوية IL - 6, TNF - α, IFN - α, CD8, and CD56))عند الاصابة بفيروس نظير الانفلونزا بواسطة فحص الامتصاصية المناعي المرتبط بالانزيم وباستخدام تقنية التالق المناعي المباشر لفيروس نظير الانفلونزا بانواعه الثلاثة (hPIV 1,2,3) كانت نسبة الاصابة 64% (اي 32 طفلا من اصل من اصل50) وعند استخدام فحص تفاعل سلسلة انزيم البلمرة بالوقت الحقيقي ( (RT - PCR وجد ان نسبة الاصابة بفيروس نظير الانفلونزا بانواعه الاربعة (hPIV_1,2,3 and 4) 44% ( اي 22 من 50) تضمنت الدراسة استخدام المجهر الالكتروني النفاذ لرؤية جزيئة فيروس نظير الانفلونزا في افرازات الاذن . اظهرت نتائج التشخيص البكتيري ان نسبة الاصابة بالمسبحيات القيحية هي 14%(7 من 50) بعد زرعها على وسط اكار الدم وحضنت لمدة 24 ساعة بدرجة 37 وتم فحصها بواسط المجهر الضوئي بعد تصبيغها بصبغة كرام , تم التشخيص بواسطة استخدام فحص Bacitracin sensitivity وفحص انتاج انزيم الهيمولايسين على وسط الدم بواسطة فحوصات الكيموحيوية وفحص Vitek 2 systemاظهر التشخيص الجزيئي للكشف عن وجود mga جين ان جميع العزلات تحتوي على هذا الجين اي نسبة 100% .كما اظهرت الدراسة ان نسبة وجود الفيروس مع البكتريا في في سوائل الاذن بنسبة (16%)( . 4 وكانت نسبة وجود الفيروس (72%) 18وشملت الدراسة ايضا فحص الحركيات الخلوية بواسطة فحص الامتصاصية المناعي المرتبط بالانزيم واظهرت النتائج ارتفاع مستوى السايتوكينات في مصل مرضى التهاب الاذن الوسطى مقارنة مع الاشخاص الاصحاء وكان اعلى مستوى لانترليوكين 6 في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو) 88.24 )و (100.95) 1Pg/ml على التوالي . في حين كان اعلى مستوى لعامل النخر الورمي نوع الفا في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو (99.35 ) و(109.81 ) Pg/ml على التوالي. وكان اعلى تركيز لانترفيرون الفا في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو( 217.16) و(( 197.84 Pg/ml على التوالي . واظهرت النتائج ان تركيز الواسمين المناعيين CD8 وCD56 ان تركيزهما اعلى في مصل مرضى التهاب الاذن الوسطى مقارنة مع الاشخاص الاصحاء , اظهرت النتائج ان تركيز CD8 في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو ((11.46±1.49 و(14.76±1.73) ng/ml على التوالي . في حين كان تركيز CD65 في امصال مجموعة المرضى المصابين بفيروس نظير الانفلونزا للذكور والاناث هو (12.82±1.62 )و (10.59±1.19) ng/ml على التوالي | This study aimed to detect the role of of parainfluenza virus and Streptococcus pyogenes in children suffering from suppurative otitis media. A total of two hundred sample (three swab for each patients include ear swabs , nasopharyngeal secretion for viral study and ear swab for bacterial study and 50blood sample) , were collected from 50 patients suffering from suppurative otitis media attending to AL - Hilla Teaching Hospital in Babylon - Iraq during the period from February 2016 to May 2016. The study includes 35 samples obtained from healthy subject as control group that classified to six group according to age in similar way of patient's with parainfluenza virus infection group years old The study includes four main parts .First one is the viral diagnosis based on relied diagnostic procedure ( Primary detection of parainfluenza virus in ear discharge by direct Immunofluorescence for parainfluenza virus 1, 2 and 3 types, molecular diagnosis by Real - Time PCR technique for the qualitative detection and differentiation of Parainfluenza Virus 1, 2, 3, 4 and diagnosis of parainfluenza virus by transmission electron microscope. The second part includes the bacterial diagnostic based on relied diagnosis procedure (using conventional biochemical reaction and commercial kit (Vitek 2 system). The heamolysis on blood agar was also observed in all isolates. Molecular detection of mga virulence gene for Streptococcus pyogenes was detected using conventional PCR technique. The third part is Co - infection between GAS and HPIV in OM specimens of children with suppurative OM. The fourth part is an immunological study to investigate the role of cytokines (IL - 6, TNF - α, IFN - α, CD8 and CD56) in HPIV infection by ELISA assay . Direct immunofluorescence for parainfluenza virus 1, 2 and 3 types antigen was positive in 64% (32 out of 50) , when we use Real - time reverse transcription PCR(RT - PCR) the percent of children infection with HPIV1, 2, 3, and 4 was 44% (22 out of 50). The study also include the use of transmission electron microscope for visualized the parainfluenza virus particle in ear swab. Streptococcus pyogenes isolates was detected in 14%(7 from 50) the isolate were confirmed after culturing on the blood agar and incubated for 24 hour at 37ºC, then microscopically by Grams̕̕ staining. Identification was done using commercially prepared bacitracin and the type of hemolysis on blood agar by primary biochemical test and confirmatory identification by vitek2 system compact. Molecular Identification of mga gene for Streptococcus pyogene showed that all Streptococcus pyogenes positive result 100%. The occurrence of Bacterial and viral co - infection in middle ear fluids which have been observed in 16% (4 out of 50 cases). HPIV was identified as a single agent in ear swab in 72%(18 out of 50). The study include estimation of some immunological parameter include (IL - 6, TNF - α, IFN - α , CD8 and CD56 ) using ELISA and revealed elevation the concentration of cytokines in sera of otitis media patients in comparison with healthy control groups. The concentration of IL - 6 is higher in sera of otitis media patients with parainfluenza virus than healthy control group. The high level of IL - 6 Pg/ml in otitis media patients for male and female was (88.24) and (100.91) Pg/ml respectively. Also level of TNF - α Pg/ml in otitis media patients for male and female was (99.3) and (109.8) Pg/ml respectively . The level of IFN - α Pg/ml in otitis media patients for male and female was (217.16 )and (197.84 )Pg/ml respectively. The results also show elevation concentration of CD8 and CD56 in sera of otitis media patients with parainfluenza virus than healthy control group. For CD8 ng/ml in otitis media patients for male and female was(11.46) and (14.76)ng/ml respectively. The level of CD56 ng/ml in otitis media patients for male and female was (12.82) and (10.59)ng/ml. respectively.

الكشف عن اللولبية البوابية في خزعة المعدة ودراسة مستوى P53 وطفرات مورثة Bax المرتبطة بالقرحة الهضمية == Detection of Helicobacter pylori in Gastric Biopsy and Study the Level of P53 and Bax Gene Mutations Associated With Peptic Ulcer

اسم المؤلف: اصيل هاشم راضي السعبري
اسم المشرف: محمد صبري عبد الرزاق السعيد | مشتاق عبد العظيم وتوت
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: اللولبية البوابية هي بكتريا سالبة لصبغة كرام, الذي تصيب غالبية سكان العالم وتسبب امراض مختلفة مثل التهاب المعدة المزمن, القرحة الهضمية وسرطان المعدة. في حين ان غالبية الناس المصابين بالبكتريا اللولبية لا تظهر عليهم اعراض مرضية. ان العوامل الرئيسية التي تحدد تطور الامراض ذات الصلة بالبكتريا اللولبية قد تكون ضراوة البكتريا, وعوامل المضيف الوراثية والبيئية.الهدف من هذه الدراسة هو الكشف عن اللولبية البوابية في انسجة المعدة ودراسة عوامل الضراوة الرئيسية لهذا الممرض والعوامل الوراثية للمضيف في المرضى الذين يعانون من اعراض اصابات المعدة والاثني عشري. وتشمل الطرق الجزيئية ايضا تفاعل سلسلة البلمرة (PCR) للكشف عن جينات التشخيص والضراوة لبكتريا اللولبية البوابية وتقنية (SSCP) تم القيام بها للكشف عن الطفرات الجينية ل P53 وال Bax جين التي قد تترافق مع عدوى اللولبية البوابية.بلغ العدد الكلي للنماذج (الخزعات) المعوية 180 نموذج. فقط 92 خزعة معدة (51 ٪) اعطى نتيجة ايجابية لللوليبيات البوابية (Helicobacter pylori ) للتشخيص بواسطة التقنية الجزيئية, مع ذلك من بين هذه النتائج الايجابية 60% منها اعطى نتيجة ايجابية للزراع, التي تعتمد على الميزات الكيميائية الحيوية. كما تم اخذ 100 عينة مصل الدم من افراد اصحاء مع عدم وجود تاريخ عدوى بالبكتريا اللولبية كمجموعة سيطرة ونتائج اختبار الاجسام المضادة على اساس (اختبار المصلية) اعطت نتائج سلبية للبكتريا اللولبية.قد تم ملاحظة وجود تكرار عالي للولبية البوابية بالتهاب المعدة المزمن الذي يشكل 88٪ بينما التكرار القليل كان مقداره 3٫3٪ لسرطان المعدة في حين ان 8٫7٪ كان لقرحة الاثنى عشري. وقد كان التكرار العالي لعدوى اللولبية البوابية بين المصابين الذكور اكثر من الاناث, ويعود سبب ذلك لعدة عوامل مثل (متوسط العمر الاجتماعي والاقتصادي ونقص التغذية وسوء النظافة) ويعتبر التدخين احد العوامل الاضافية المرتبطة مع انتشار عدوى (اللولبية البوابية ) بسبب السلوكيات غير الصحية المرافقة للتدخين , والتي قد تشمل نقل البكتيريا من خلال اللعاب بين المدخنين. من جهة اخرى اظهرت النتائج ان معدل العدوى العالي للمجموعة العمرية (31 - 50) سنة الذي تم تسجيله كان 44٫6٪. علاوة على ذلك فان الكشف عن اللولبية البوابية بتقنية سلسلة تفاعل البلمرة PCR)) قد اجريت على جميع انواع خزعات المعدة وجين (ure C ) اضافة الى جين (ure A ) اللذان يستخدمان كعلامات للتشخيص التاكيدي للولبية البوابية من خلال استخدام (primers ) خاص لتقنية PCR. وضحت النتائج ان 84٫8٪ و73٫9٪ للولبية البوابية كانت ايجابية لكلا الجينين. للعينات التي اعطت نتائج ايجابية (للتضاعف الجيني amplification ) بواسطة (PCR ), كان 56 عينة ما يمثل (60٪) قد اعطت استجابة لنتائج الزرع. اضافة الى التضاعف بواسطة PCR لعلامات جين (ure C ) وجين (ure A ). اضافة الى ذلك ,المعدل الايجابي للولبية البوابية للخزعات تحت الاختبار كان 51٪ بالاعتماد على الكشف الجزيئي المباشر بواسطة (PCR) باستخدام جين (ure A) وجين (ure C ). علاوة على ذلك, تم فحص جين (cag A) لكل عينات اللولبية البوابية التي تم عزلها. اشارت النتائج الى ان65 عزلة (70٫6٪) كانت ايجابية لتضاعف (PCR ) للجين من نوع cagA) ) حيث ان عزلة 56 (60٫8٪) كانت ايجابية لتضخيم (PCR ) للجين من نوع (cag E ). من بين النتائج الايجابية التي تم عزلها 49 عزلة (53٫3٪) كانت قد اعطت نتائج ايجابية للجين من نوع (cag A ). كذلك وجد تكرار الجين (vac A ) مقداره في الدراسة الحالية 68٫5٪ (63/92). اضافة الى كل اللولبيات البوابية الايجابية لخزعة المعدة 49 (53٫3٪) اظهرت اشارة كاملة للجينات نوع (cagA, cagE وvacA (. بالنسبة للعينات المعدية المتبقية 27 عينة (30٫4٪) التي تحوي سلالات سلبية كانت سلبية للجين (cag E). وقد تم تحديد طفرات جين (p53) في الاكسونات 5 و6و 7 و8 باستخدام طريقة SSCP. ومع ذلك تم استخدام PCR لتضخيم الاكسونات 5 و6 و7 و8 للجين (p53) التي من المعروف انها بقع ساخنة طافرة .تحليل PCR - SSCP يكشف تعديلات في الجين (p53) في عدة اكسونات. حيث ان ستة عشر من التعديلات في الاكسون E5A , سبعة عشر التعديلات في الاكسون E5B6A, وكذلك سبعة عشر التعديلات في الاكسون E7 وتعديل واحد فقط في الاكسون E6B لكن لم يظهرتعديل في الاكسون E8 في الجين (p53). بنفس الاسلوب ان تحليل PCR - SSCP كشف عن تعديلات في جين bax في الاكسونات E1 وE4 وE6. ويظهر ذلك في الاشكال. فيما يخص الاكسون 1 في bax يمكن ملاحظة وجود 6 نماذج فقط تعطي طفرات موضع ايجابية للجين. قد يعزى ذلك الى اللولبية البوابية التي تحفز طفرة bax من خلال قابليتها على انتاج بروتين cag A الذي له القابلية على تغيير التسلسل الجيني والوظيفة. واستنتجنا الى ان التشخيص الجزيئي قدم دليلا توكيديا على وجود اللولبية البوابية في عينات المعدة. ايضا قد تؤثر السلالات الاكثر ضراوة على تحفيز طفرات جينية في جينات ال p53 وال bax. | Helicobacter pylori is a gram - negative bacteria which infects a majority of the world population. It causes various diseases such as chronic gastritis, peptic ulcer and gastric cancer. While majority of the people infected with H. pylori is asymptomatic. The main factors, which determine the development of H. pylori related diseases might be bacterial virulence, host genetic and environmental factors.The aim of this study is detection of H. pylori in gastric tissue and study the main virulence factors of this pathogen and host genetic factors in patients with gasteroduodenal manifestation. Molecular methods include polymerase chain reaction (PCR) for diagnosis and virulence genes of H. pylori and single strand confirmation polymorphism (SSCP) technique was done to detect the p53 and bax genes mutation may be associated with H. pylori infection. In this study, 180 patient were included, only 92 gastric biopsies (51%) gave positive for the presence of H. pylori diagnosed by direct molecular technique. Among these positive result, 60% gave positive results in cultivation based on biochemical features. In addition, 100 serum samples were taken from healthy individuals, with no history of H. pylori infection as a control group and the results of antibody based test (serological test) were negative for H. pylori.In this study, the high frequencies of H. pylori were observed for chronic gastritis, which constituted 88% while low frequencies were 3.3% for gastric cancer, whereas 8.7% for duodenal ulcer.Also, the highest frequencies of H. pylori infection were found in male patients in comparison to female. On the other hand, the results showed that the high significant infection percentage 44.6%, among age group ranging from 31 - 50 years. Moreover, detection of H. pylori by Polymerase Chain Reaction (PCR) technique is performed on all gastric biopsy. ure C as well as ure A genes are used as markers for confirmatory diagnosis of H. pylori through using specific primers of PCR technique. The results show that 84.8% and 73.9% of H. pylori are positive for both genes, respectively. Among samples with positive result for PCR amplification, 56 samples (60%) also had positive culture result. Besides, the PCR amplification of ureC and ureA markers. Also, the rate of positive H. pylori in the tested biopsies was 51% depending on direct molecular detection by PCR using the ureA and the ure C genes.In addition, cag A gene is investigated in all H. pylori isolates. The results show that 65 isolates (70.6%) are positive for PCR amplification of cagA gene whereas 56 isolates (60.8%) are positive for PCR amplification of cag E gene. Among of these positive isolates, 49 isolates (53.3%) give positive result for cag A gene. The frequency of the vacA gene found in the present study is 68.5% (63/92).Furthermore, among of all H. pylori positive gastric biopsy, 49 (53.3%) reveal full signals of cagA, cagE, and vacA. The remaining 27 (30.4%) gastric biopsies, harboring cagA - negative strains, are PCR negative for cagE.Mutations of the p53 gene in exons (5,6,7 and 8) are identified by PCR - SSCP method. However, PCR is used to amplify exons 5,6,7 and 8 of p53 gene which are known to be mutational hot spots. PCR - SSCP analysis has detected alterations in the p53 gene in several exons. Where sixteen alterations in exon E5A, nineteen alterations in exon E5B6A, seventeen alterations in exon E7 and only two in exon E6B but no alteration is seen in exon E8 of P53 gene.In the same way, PCR - SSCP analysis detected alterations in Bax gene where only 17% of positive samples gave alteration in the sequence of Bax gene in exons E1, E4 and E6. Regarding exon 1 of Bax gene, it was seen that only 6 samples gave positive gene locus mutation. It conclude that the molecular diagnosis gave an evidence and confirmatory guide on the existence of H. pylori in gastric samples. Also the more virulent strain may be affected on the stimulation of p53 and bax genes mutation.

فاعلية البرنامج التثقيفي على معارف وممارسات العاملين الصحيين تجاه السيطرة على العدوى في مراكز الرعاية الصحية الاولية في مدينة كربلاء المقدسة == Effectiveness of an Education Program on Health Care Workers' Knowledge and Practices toward Infection Control at Primary Health Care Centers in Holy Karbala City

اسم المؤلف: سلمان حسين فارس الكريطي
اسم المشرف: اركان بهلول ناجي
الموضوع العام: الطب
السنة: 2017
الموضوع الدقيق: التمريض
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: دراسة شبه تجريبية اجريت في مراكز الرعاية الصحية الاولية في مدينة كربلاء المقدسة للفترة من اذار 2102 . لغاية نهاية شهر نيسان 2102 لقد هدفت الدراسة الى تقييم فاعلية البرنامج التثقيفي على معارف وممارسات العاملين الصحيين تجاه السيطرة على العدوى في مراكز الرعاية الصحية الاولية في مدينة كربلاء المقدسة. تم تقييم الاحتياج للبرنامج التثقيفي، واظهرت نتيجة التقييم وجود ضعف في معارف وممارسات العاملين الصحيين تجاه السيطرة على العدوى في مراكز الرعاية الصحية الاولية.) شملت عينة الدراسة ) 22 عاملا صحيا اختيروا بشكل غير عشوائي )عمدي( ، وقد قسمت العينة الى مجموعتين مجموعة الدراسة ) 22 ( عاملا صحيا، والمجموعة الضابطة ) 22 ( عاملا صحيا . تم بناء برنامج تثقيفي حسب احتياج العاملين الصحيين واعدت ثلاث استمارات واحدة للمعلومات الخاصة بالعينة لتحديد صفاتها، واستمارتين لتقييم معارف وممارسات العاملين الصحيين ، استعملت للاختبار القبلي والبعدي. تم تحقيق مصداقية الاداة بعرضها على مجموعة خبراء لمراجعتها وتحديد درجة مصداقيتها، وتم تحديد ثبات اداة القياس من خلال الدراسة التجريبية.تم الاختبار القبلي لافراد المجموعتين ومن ثم تطبيق البرنامج التثقيفي لمجموعة الدراسة فقط للفترة من التاسع عشر لغاية الثالث والعشرين من حزيران 2102 ، تم الاختبار البعدي الاول مباشرة بعد تنفيذ البرنامج، ثم الاختبار البعدي الثاني بعد اربعة اسابيع من الاختبار البعدي الاول.تم تحليل النتائج باستخدام الاحصاء الوصفي والاحصاء الاستدلالي. اظهرت النتائج استفادة العاملين الصحيين بعد تطبيق البرنامج التثقيفي حيث الزيادة في مستوى المعرفة والممارسة للعاملين الصحيين وان البرنامج التثقيفي الخاص بالسيطرة على العدوى كان فعالا في تحسين معارف وممارسات العاملين الصحيين المشاركين فيه وعدم وجود اختلافات هامة بين مستوى تحسين معارف وممارسات العاملين الصحيين نتيجة لتطبيق البرنامج طبقا للعمر، المستوى التعليمي، سنوات الخبرة والمشاركة في الدورات التدريبية في الاختبار البعدي لمجموعتي الدراسة والضابطة باستثناء سنوات الخبرة في مجموعة الدراسة فقد اظهرت النتائج وجود اختلافات هامة بين مستوى تحسين معارف العاملين الصحيين في الاختبار البعدي لمجموعة الدراسة .واوصت الدراسة بتوفير الادلة الارشادية والتاكيد على اهمية التعليم المستمر للعاملين الصحيين باستعمال الممارسات المعتمدة على الادلة لتطبيق الاحتياطات القياسية للسيطرة على العدوى. وان هنالك حاجة لاكثر من برنامج تثقيفي حول السيطرة على العدوى في كل مراكز الرعاية الصحية الاولية ولعينة اكبر لتستفيد من النتائج . | A quasi experimental study was carried out at the primary health care centers in holy Karbala city from March 2016 to the end of April 2017.The study aims to assess the effectiveness of an educational program on the knowledge and practices of healthcare workers in Karbala city. Assessment of needs was done to evaluate the needs for the educational program. The result of the assessment revealed that the healthcare workers had deficit knowledge and practice regarding infection control in primary health care centers. A Non - Probability sample of (52) healthcare workers was selected randomly and divided into two groups : (26) considered as a study group and the other (26) considered as control group. An educational program was constructed, and three formats were developed one for assessing demographic characteristics of sample, the other two for assessing healthcare workers knowledge and practice. Validity of the instrument was determined through content validity by a panel of experts, and reliability of the tool was determined through a pilot study.A pre - test was done for both groups; educational programs were applied only for the study group from June 19th to June 23rd 2016. Then the first post - test was applied after the implementation of the program and the follow up after four weeks from 1st post test.Analysis of data was performed through the application of descriptive and inferential statistics.The findings revealed that the healthcare workers of the study group had benefited from the implementation of the educational program; their knowledge and practice were significantly improved comparing with the control group, the infection control education program was found to be effective in knowledge and practices improvement to the participant HCWsand non - significant association between HCWs knowledge and practice improvement effect result by application of the education program and their age, education, years of experience and training session in post - test I for study and control group. Except for years of experience in study group it shows a significant association with knowledge improvement.The study recommends the availability of written guidelines and focus on the importance of continuing education for HCWs using the evidence - based practices for application of standard precautions of infection control; and more educational programs of infection control are needed in all PHC centers and on a larger sample to benefit from the results.

تاثير الرش بالسليكون في تحسين التحمل الملحي لصنفين من الطماطة Lycopersicon esculentum Mill المحدودة النمو == Effect of spray silicon on salinity tolerance improvment of two tomato (Lycopersicon esculentum Mill.) cultivars growth limited

اسم المؤلف: ناصر جبير راضي حسين الحسناوي
اسم المشرف: مؤيد فاضل عباس | عواطف نعمة جري
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: النبات - البستنة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: This experiment was conducted during two growing season (2015 and 2016) in private farm in Kufa - Najaf, with the objective of improving salinity tolerance of tomato (Lycopersicon esculentum Mill.) with application of silicon. The study included 40 treatments, while were four levels of irrigation water salinity (2.5, 5.0, 7.5 and 10 dS.m - 1) , two tomato cultivars (Aulla and Majid) and five concentration of silicon (0 , 50 , 100 , 150 , and 200 mg l - 1). A split - split plot design was used in a factorial experiment with three factors and three replicates. The results were analyzed using analysis of variance and treatment means were compared using the least significant differences (LSD) at 0.05.The effect of the above treatment were studied on vegetative growth parameters, chemical constituents of leaves, flowering parameters ,yield and yield components, as well as fruit chemical parameters. The results may be summarized as follows : 1 - vegetative growth parameters : Irrigation water salinity at 5, 7.5 and 10.0 dSm - 1 caused a significant decrease in plant length ,number of side branches, leaf number, leaf area as well as fresh and dry weights of the shoot system, for both growing seasons. Treatment with silicon caused a significant increase in all vegetative growth parameters, for both growing seasons. As for the effect of cultivars, the cultivar Majid was superior in all vegetative growth parameters, as compared with cv. Aulla, for both growing seasons. As for interactions among treatments, they were significant in their effects on vegetative growth parameters and for both seasons.2 - chemical constituents of leaves : Irrigation water salinity treatments at 7.5 and 10.0 dS m - 1 caused a significant increase in the concentration of sodium and chloride ions, proline, as well as the plant hormone ABA and potassium ion leakage. However, such treatments caused a significant decrease in K+, Ca++and Si ions total solute carbohydrates and total chlorophylls, and the concentration of both auxins and gibberellins and K+ : Na+ ratio.As for treatment with silicon, at 200 mg l - 1 caused a significant increase in total soluble carbohydrates, total chlorophylls, auxins , gibberellins as well as K+,Na+ and Si ions and for both growing seasons. However, the sane treatment led to a significant decreased in the concentration of Na+ and Cl - ions as well asbfree proline concentration, and ABA levels. Treatment with silicon al so caused a significant increase in K+ : Na+ ratio, but decreased potassium ion leakage. As for the effect of cultivar plants of the cv. Majid had a significantly higher levels of most chemical constituents as compared with plants of the cv. Aulla, with the exception of the concentration of Na+, Cl - , ABA and gibberellins wheres plants of the cv. Majid had the higher levels. As for interactions among treatments, they were significant as there was an increase in the concentration of K+, Si , total soluble carbohydrates, total chlorophylls, auxins , gibberellins and K+ : Na+ ratio for both growing seasons.3 - flowering parameters, yield and yield components : Irrigation water salinity at 7.5 and 10.0 dS.m - 1 a significantly reduced the number of inflorescence, total number of flowers per inflorescence as well as percentage fruit set. This treatments also caused a significant reduction in yield components, represented by number of fruit, mean fruit weight, yield per plant and total yield for both growing seasons. Silcon treatment at 50 ,100,150,200 mg L - 1 significantly increased total marketable yield as much as (18.16, 25.12, 37.18 , 35.82) for first season and (19.26, 30.05, 35.83, 29.09) as compared with untreated treatment for second season, respectively. For cultivars, the cultivar Majid was significantly superior in total marketable yield as much as (19.65, 20.66 and %), as compared with cv. Aulla for both growing seasons respectively. The interactions among treatments, were significant in increasing flowering growth parameters, as well as yield and its components.4 - fruit chemical parameters : Irrigation water salinity at 7.5 and 10.0 dS.m - 1, caused a significant reduction in ascorbic acid concentration and concentration of β - Carotene and Lycopene, it caused a significant increase in total soluble solids, titratable acidity and, percentage dry matter, as well as the. As for application of silicon, it caused a significant increase in all chemical parameters. The cultivar Majid had a significantly higher chemical constituents as compared with the cultivar, Aulla. The interactions among treatments, were significant in increasing the chemical constituents of the fruits.

تاثير اضافة بعض الاحماض الامينية وعصير البنجر الى المخففات في بعض صفات السائل المنوي المبرد والمجمد للكباش == Effect of adding some amino acid and Beet juice to the dilutors on some cooling and freezing rams semen traits

اسم المؤلف: فلاح عبد المحسن عبد الله الاسدي
اسم المشرف: عماد فلاح حسن | عبد الرزاق نعيم خضير
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: الحيوان
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The present study was conducted at the agricultural experimental Research Station / College of Agriculture / University of Basrah form 3rd /1/2015 to 3rd /1/2016. A total of 12 mature rams (4 Najdi , 4 Arabi and 4 crosses) aged 3 - 3.5 years with nearly similar weights . Nutrition depended on concentrate ration ( 47 % wheat bran , 50 % barely ,1% urea , 1% CaCo3 and 1% Nacl ) , with 16.58 % crude protein and TDN 72.17% and was given 0.5kg /animal twice aday (at morning and afternoon)500 - 750 g / animal green fodder with wheat straw was given as it was available in the farm , cubic mineral and water provided freely at the day. Rams were training for semen collection for 30 days before the beginning of the study by using artificial vagina used for sheep. Semen was collected three time each month. The study includes two experiments : First experiment : It was designed to study the effect of breed , season ( spring , summer , autumn and winter ) and month on semen characteristics physical , sexual hormones ( testosterone , ICSH and SSH) of blood serum and level of enzymes ( AST , ALT and ALP ) of blood plasma.Second experiment : It included ten diluters from local materials (Beet pulp juice ) with 5 , 10 and 15% egg yolk and amino acids ( Glycin and cysteine ) with concentration of (5, 10 and 15) mmol. It also included the effect of type of dilutor , cooling , freezing storage period ( one and two months) in liquid nitrogen ( - 1960 C) on individual motility and live sperm percent .bThe results can be summarized as follow : 1 - Arabi rams exceeded (P<0.05) Najdi and crosses rams in their semen pH , spring also showed higher (P<0.05)pH followed by winter in comparison with summer and autumn .May showed higher (P<0.05) semen pH in comparison with other months.2 - Najdi rams exceeded (P<0.05)Arabi and their crosses in mean ejaculate volume, mass and individual sperm motility , sperm concentration , live sperm percent and mean testes circumference .3 - Summer and autumn showed higher (P<0.05) in meanejaculate volume , mass and individual sperm motility , sperm concentration , live sperm percent and mean scrotal circumference than winter and spring . Spring also showed higher (P<0.05) dead sperm percent followed by winter in comparison with summer and autumn .4 - August showed higher (P<0.05) ejaculate volume and mass and individual sperm motility followed July and June in comparison with other months. September , August and October exceeded (P<0.05)in comparison with other months sperm percent . October showed higher (P<0.05) in live sperm percent in comparison with other months . Highest (P<0.05) dead sperm percent happened during March followed by April and May . Scrotal circumference was high (P<0.05) during September followed by November and October.5 - Najdi and Arabi rams showed significant (P<0.05)differences in testosterone and ICSH hormones in comparison with their crosses . However , crosses exceeded (P<0.05)Arabi and Najdi rams in mean of SSH hormone. Autumn followed by summer exceeded (P<0.05) wintercand spring in testosterone , ICSH and SSH hormones. September and October showed higher (P<0.05)testosterone, ICSH and SSH hormones in comparison with other months.6 - Arabi rams exceed (P<0.05) both Najdi and their cross by enzymes concentration ( AST, ALT and ALP) of semen plasma . Spring and winter exceeded (P<0.05) summer and autumn by mean of ALT and AST enzymes. However , summer and winter exceeded (P<0.05) autumn and spring by mean of ALP concentration . March showed highest (P<0.05)AST and ALT concentration , while August showed highest (P<0.05) ALP concentration.7 - Amino acid diluter ( 15 mmol glysin and cysteine ) and 10 % beet pulp juice showed highest (P<0.05) individual motility after cooling and freezing for month and two monthsduring all months of year. While the beet pulp juice 15% and control showed the lowest means (P<0.05) of individual motility after cooling and two months of freezing.8 - Glysin 10 and 15 mmol , cysteine 10 mmol and beet juice 10% showed significant (P<0.05) increase in live sperm percent after and cooling and freezing . On other hand beet pulp juice 15% and control recorded the least value (P<0.05) . Beet pulp juice and control showed the highest (P<0.05) level of dead sperm percent after cooling and freezing in contrary with glysin 10 mmol and cysteine 15 mmol

تاثير اضافة الخليط التازري لمسحوق حليب الكيفير المجفف والمصنع في بعض الصفات الانتاجية والفسلجية والمناعية لفروج اللحم == Effect of Addition of Synbiotic of Dried and Processed Kefir Milk Powder in Some of The Productive, Physiological and Immunological Characteristics of Broiler Chicken

اسم المؤلف: عقيل طوينة عودة
اسم المشرف: عبد الله عبد المنعم محمد
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: الاغذية
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Four experiments were carried out for this study, two field and two laboratory, to manufacture a Synbiotic for milk powder Kefir ( MPK ) and loaded with some feed material with or without sugar beet milled or Helianthus tuberosus powder and the effect of adding it to some of the production , physiological and immunological characteristics of the chickens . Where the first laboratory experiment was conducted in Microbiology Laboratory at the Animal Production Department in the College of Agricultur - University of Basrah , for the period from 10 / 4 / 2015 to 7 / 5 / 2015 for the purpose of manufacturing a Synbiotic of MPK.The second field experiment was conducted in a commercial field for poultry in Al - Zubair / Basrah during the period from 24 / 1 / 2016 to 27 / 2 / 2016 , which included selecting the best biomass of MPK loaded on wheat , corn , soybeans and wheat bran with the addition of Helianthus tuberosus powder , So that one gram of MPK and loaded on the previous materials can bacteria . In this L. acidophiluscfu / ml 8least 10be provided at experiment, 450 unsexed one day of the Ross 308 chicks, at a rate of 40 g / chick were distributed randomly to six Treatments , three replicates per treatment , 25 birds per replicate and the treatments were as follows ; T1 : Control treatment included a standard diet . T2 : A standard diet supplemented only by amoxicillin antibiotic according to the program of preventive health approach . T3 : A standard meal with 3 g of MPK loaded on wheat/ kg feed .T4 : A standard meal with 3 g of MPK loaded on yellow corn / kg feed. T5 : A standard meal with 3 g of MPK loaded on soybeans / kg feed. T6 : A standard meal with 3 g of MPK loaded on wheat bran / kg feed .The third field experiment was conducted to determine the best effect of the synbiotic MPK loaded with wheat, corn, soybeans and wheat bran and compared with three foreign probiotics in the productive, physiological and immunological characteristics of chicks. This experiment was conductedbduring the period from 4 / 10 / 2016 until 9 / 11 / 2016, using 600 unsexed chickens with one day of the Ross 308 at a rate of weight of 40 g / chick .The chicks were randomly distributed into eight treatments with three replicates per treatment and 25 birds per replicate. The experiment was designed as follows : T1 : Control treatment included a standard diet, T2 : A standard meal with 3 g of MPK loaded on wheat/ kg feed . T3 : A standard meal with 3 g of MPK loaded on yellow corn / kg feed. T4 : A standard meal with 3 g of MPK loaded on wheat bran / kg feed. T5 : added treatment 1/2 g of probiotic Labzyme / kg feed , Korean - made , T6 : added treatment 1/2 g probiotic Biozyme / kg feed , German - made , T7 : added treatment 1/2 g probiotic Biolac / kg feed , Vietnamese made , T8 : A standard diet supplemented only by amoxicillin antibiotic according to the program of preventive health approach .The fourth laboratory experiment was conducted in Microbiology Laboratory at the Animal Production Department in the College of Agriculture, University of Basrah for the period from 6 / 3 / 2016 to 7 / 4 / 2016 for the study of antibacterial activity in the kefir milk and compare it with Biozyme , Pencilin, Gentamycin, Tetracyclin and Neomycin against Staphylococcus aureus and Escherichia coli causing bacterial necrosis with Bacterial Chondronecrosis with Osteomyelitis ( BCO ) . The results of the study included the following : First laboratory experiment ;The results of the first experiment showed that the wet biomass rate of fermented kefir milk was 70 % . This high percentage is evidence of the growth of Lactate Bacteria in this product. The results showed that wheat bran and wheat bran and corn were the best loaded significant materials (p <0.05) Compared to soybeans in terms of growth and reproduction of lactic acid bacteria. Adding Helianthus tuberosus powder increased the number of bacteria in dried kefir milk compared sugar beet milled , which reduced the number of bacteria in MPK .Second field experiment : cThe results of the experiment showed the following : 1 - There were significant increase (p <0.05)) in the final body weight rate and the body weight gain and Food conversion efficiency, as well as a significant decrease (p <0.05) In the amount of feed consumed for the sixth, third and fourth treatments, supplemented with synbiotic MPK loaded on wheat bran, wheat and corn compared with other treatments .2 - Treatments sixth , third , fourth had significant (p<0.05) decrease mortality percent as comparing with control and treatment two .3 - The sixth , third , and fourth treatments were significantly higher (P <0.05) in the production index and the performance index and drcrease value of economic efficiency compared with other treatments .4 - Total protein and globulin in plasma serum were increased significant (p<0.05) and decreased (p <0.05) in the concentration of triglycerides and cholesterol in the sixth, third and fourth treatments supplemented with synbiotic MPK , loaded on wheat bran , wheat and corn , as well as the increased of the third, sixth and fourth treatments significantly (p <0.05) in the concentration of ALP enzyme compared to other treatments .5 - The results showed that the fifth, sixth, third, and fourth treatments did not differ significantly in antibodies titer against Newcastle ( NDV) and the Comboro ( IBDV ) disease, but significantly exceeded (p <0.05) compared to the first and second treatments .Third field experience : According to the results of the second field experiment, the best three treatments were added to the synbiotic MPK and loaded on wheat bran , wheat and corn . It was compared with three foreign prpbiotics , the treatment of addition of amoxicillin antibiotic and control treatment. The results of the experiment showed the following ;d1 - The second , fourth , third and sixth treatments, supplemented with probiotic, MPK , loaded with wheat , wheat bran , corn, and labzyme were significantly higher (p <0.05) in body weight and weight gain . As well as a significant decrease (p <0.05) in the amount of feed consumed and a significant improvement (p <0.05) in the efficiency of food conversion compared to other experimental treatments .2 - The first, fifth, and seventh treatments were significantly higher (p <0.05) in the mortality percent as comparing , to other experimental treatments .3 - A significant (p <0.05) increased was observed in the production index and the performance index and significantly decrease in the economic efficiency value for the second, fourth, third and sixth treatments.4 - The second, fourth, third and sixth treatments were significantly higher (p <0.05) in dressing percentage , tibia and thoracic characteristics , as well as a significant increase (p <0.05) in the weight of the cecum compared to other treatments.5 - There was a significant increase (p <0.05) in the weight of Bursa fabricius for the first, eighth and seventh treatments, as well as a H/L ratio was significant (p<0.05) decrease in the second, third, fourth and sixth compared to other treatments.6 - Total protein and globulin in plasma serum were significant (p<0.05) increased and decrease significantly(p <0.05) in the concentration of triglyceride and cholesterol in treatments second, fourth, sixth and third , compared to other treatments.7 - The superiority of the second, third, and fourth treatment was significantly higher (p <0.05) in the concentration of ALP enzyme in plasma serum compared to other treatments.8 - The third, seventh, second, fourth, and sixth treatments recorded the highest significant mean (p <0.05) of the degrees granted to them by theeEvaluator in the flavor, tenderness and juicy compared to the fifth, first and eighth treatments .9 - The second, seventh, third, fourth, and sixth treatments were significantly higher (p <0.05) in lactic acid in intestinal fluid , as well as There were significant (p<0.05) increase in digestibility of dry matter , protein and fat in treatments fourth, second, third and sixth as compared with other .10 - The fourth, second, third, and sixth factors significantly exceeded (p <0.05) in weight and length of the tibia , bone index , ash ratio , and skeletal weight of the chickens, as well as The second, fourth, third, and sixth treatments were significantly higher (p <0.05) in the level of calcium and phosphorus in the bone compared with other .11 - The first, eighth, fifth and seventh treatments were significantly higher (p <0.05) in the rate of food passage in the gastrointestinal tract compared with other.12 - Significant increase (p<0.05) in villi length and crups depth in treatments fourth, second and third as compared with seventh, fifth, eighth and first.Fourth laboratory experiment : The results of the experiment showed no significant differences in the treatment of MPK with gentamycin in diameter (mm) inhibition of Staphylococcus aureus, Escherichia coli, Which cause BCO disease , while significantly increase (P <0.05) with Pencilin, Labzyme, Tetracyclin , Biozyme in diameter of the inhibition zone to the staphylococcus aureus and the diameter of the inhibition zone of Escherichia coli.

تصميم وتصنيع وتقييم الاداء الميكانيكي لالة زراعية من خلال وضع السماد العضوي في خنادق تحت سطح التربة وتاثيرها في بعض خصائصها ونمو وحاصل الذرة الصفراء Zea mays L == Designing , constructing and evaluating the mechanical performance for agricultural machine laying the manure under the soil surface and its effect on some of soil properties and growth and yield of corn ( Zea mays L.

اسم المؤلف: صادق جبار محسن
اسم المشرف: شاكر حنتوش عداي | محمد عبد الله عبد الكريم
الموضوع العام: علوم الزراعة
السنة: 2017
الموضوع الدقيق: التربة والمياه
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: ditch opener and manure laying machine was designed and manufactured in the agricultural machines and equipment dept., Agriculture college, Basrah university in 2015. This machine was evaluated to determine its mechanical performance and its ability in laying manure in ditches under the soil surface as well as the effect of the added manure on the soil physical and chemical properties, corn crop growth and production parameters.The machine consists of a frame, manure tank, chemical fertilizers tank, subsoiler for soil disturbance down to 80 cm, two boards for soil digging out to open the ditch , two boards to return the soil to ditch to bury the manure , manure and chemical fertilizers feeding mechanisms and blades for manure mixing with the soil. Also its provide with two pipes to transfer the chemical fertilizers to the soil and a petrol engine to operate the two feeding mechanism.The machine disturbed the soil by the subsoiler and digging different ditches of different depths by two boards . In additional to that it can lay the manure at different levels in the opened ditches using the feeding mechanism and thereafter buried the manure in the soil . The manure can be laid under the soil as separate layer or mixed with soil using the mixing blades.Three experiments were conducted to evaluate the machine performance. These experiments are : (1) - Laboratory experiments were conducted to study the effect of the machine forward speeds (0.32, 0.46 and 0.63 m sec - 1), manure feeding mechanisms rotating speeds (360, 440, 560, 710 and 880 rpm) and two manure feeding openings areas (0.024 and 0.048 m2) as well as the interaction of the above parameters on the amount of the manure per hectare (ton ha - 1). These results were analyzed using CRD and the statistical program (Genstat). Mean results were compared using least significant difference (RLSD) at 0.05 level.The results showed the manure amount per hectare significantly increased as the rotating speeds of the feeding mechanism increased,Bthe forward speeds decreased and the feeding opening areas increased. The highest value was recorded for forward speed of 0.32 m sec - 1 , rotating speed of 880 rpm and feeding open area of 0.048 m2. These means are 64.17, 62.06 and 54.06 ton ha - 1 respectively. while the forward speed of 0.63 m sec - 1 , rotating speed of 360 rpm and the feeding open area of 0.024 m2 gave the lower values which they are 26.91, 30.04 and 33.75 ton ha - 1 respectively. Also, the forward speed of 0.32 m sec - 1 , the rotating speed of 880 rpm and the feeding area of 0.048 m2 recorded the highest amount of manure per hectare (110.00 ton) while the forward speed of 0.63 m sec - 1 , rotating speed of 360 rpm and manure feeding area of 0.024 m2 recorded the lowest value of manure per hectare (4.00 ton).(2) - A field experiment was conducted in silty loam soil in the agriculture college field at Garmit Ali location to study the effect of the implement operation depths (10, 20, 30, 40 and 50 cm), the angle between the soil digging out boards (45⁰ and 60⁰) two soil types namely cultivated soil before one year and uncultivated soil and their interaction on the implement mechanical performance parameters . These parameters are the draft force, soil disturbed area and the energy utilization efficiency (EUE) as well as the draft force requirement of the implement units (subsoiler, ditch opening boards, soil returning boards and soil mixing blades). The results were analyzed using RCBD. The analyzing was carried out by the statistical program (Genstat). Mean results were compared using RLSD at probability level of 5% , and showed the followings : (2 - 1) - The draft force, the disturbed area and the energy utilization efficiency increased significantly when the operating depth increased from 10 to 50 cm. The amount of the increase is 26.66 kN , 0.4111 m2 and 7.67 m3 mJ - 1 respectively.(2 - 2) - The angle between the opening boards of 60⁰ significantly surpassed angle of 45⁰ in increasing the draft force, the disturbed area and EUE. The percentage of increase is 12.55 , 21.93 and 10.85 % respectively.C(2 - 3) - The draft force decreased while the disturbed area and EUE increased by 16.30, 14.91 and 20.72 % in the disturbed soil compared with undisturbed soil respectively.(2 - 4) - The draft force requirement of the subsoiler was higher than that for the other implement parts. The share of the draft force requirement of the subsoiler was 60 % out of the total draft force requirement of the implement while it was 26, 9 and 5 % for the ditch opening boards, the soil returning boards and soil mixing blades respectively.(2 - 5) - The draft force requirement of all implement units increased significantly with implement operating depths and the angle between the ditch opening boards and it was higher in the uncultivated soil compared with the cultivated soil. The draft force requirement of the soil returning boards and the soil mixing blades in both soil types was not significantly different.(3) - Another field experiment was conducted in the field of the agriculture college at Garmit Ali location in the season of 2015 in a cultivated soil of silty loam texture. The aims of the experiment were to study the effect of the added manure level (0, 20 and 40 ton ha - 1), the depths of added manure (10, 20 and 30 cm) and the methods of the addition (subsoil layer and mixing with soil) and their interaction on the soil properties and corn crop (Zea mays L.) growth parameters and yield at the end of the growth season.The soil was plowed and harrowed (soil clods breaking down). The manure was added thereafter by the implement in the soil according to the studied treatments. The corn seeds were sown in rows at 1/7/2015. The drip irrigation method was used to irrigate plants until the end of the experiment 10/10/2015. The other agricultural operations were conducted according to methods used in the around area.Soil and plants samples were collected for analysis and the corn seeds yield was calculated at the end of the growth season. The measurements included soil bulk density, soil moisture content, soilDsalinity, soil pH, the dry weight of the shoot, N, P, K uptake in shoot and the total seeds production.The split - split plot in RCBD design was used to analyze the means using Genstst program. Mean results were compared using RLSD at probability level of 5 %, and showed the followings : (3 - 1) - The manure level of 40 ton ha - 1 surpassed the other two levels (0 and 20 ton ha - 1) in giving higher values of soil moisture content, higher uptake of N, P, K, a greater amount of dry weight and total yield while it gave lower soil bulk density, soil salinity and soil pH.(3 - 2) - Increasing the depth of added manure from 10 to 30 cm with the mixing method lead to significant increase in the soil moisture content, uptake of N, P, K, the dry weight and the total yield production. Whereas the soil bulk density, soil salinity significantly reduced while the soil pH was not significantly affected.(3 - 3) - Mixing of 40 ton manure ha - 1 at depth of 30 cm gave the highest crop growth and yield production which they are 13.94 and 6.75 ton ha - 1, respectively.

منهج المروزي وموارده في كتاب طبائع الحيوان : مقالات منتخبة من المقالة الاولى باب الصين والترك والهند == Al - Marowzi Approach And Its Resources In The Book Of Tba'E Alhaywan Selected From The First Article Chapters Of China, Turk And India

اسم المؤلف: غازي هادي حمزة اليساري
اسم المشرف: هاشم ناصر حسين الكعبي
الموضوع العام: التاريخ
السنة: 2017
الموضوع الدقيق: التاريخ الاسلامي
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: هذه الاطروحة تقدم المؤرخ والجغرافي والطبيب شرف الزمان طاهر المروزي - كان حيا ( سنة 518هـ/ 1134م ) - وبعنوان ( منهجية المروزي في كتاب ابواب الصين والترك والهند , ابواب منتخبة من كتابه طبائع الحيوان وموارده ) , وهذه الدراسة تاتي ضمن حقل الدراسات التاريخية و| This thesis of progress and geographic and doctor sharaf al - zaman tahir al - Marowzi - was alive (year 158 AH \ 1134 AD) - And entitled (Al - Marowzi methodology in the book of the Chapters of China and Turk and India , Elected chapters of his book Tba'e Alhaywan and resources) , This study is part of the historical and geographical studies field That are interested detects historians Arab Muslims efforts , And to clarify their approach to authoring and resources and their tactics in dealing with the events witnessed by the Arab Islamic nation , And the date of their neighboring nations and clans , Hence the desire to record the subject came , and the approach of Al - Marowzi was in the writing of the three chapters - China and Turk and India - particularly strong motivation towards writing this thesis. Sharaf al - zaman tahir al - Marowzi considered is one of the leading historians of Arab Muslims who wrote about the Levant and Morocco typing known Tba'e Alhaywan - The first article of it - And the known world of his time , The polls and note down what is received from this historic Nations resources , Especially the subject of our study - The country of China, India and Turk - and He writes what is being offered to him and raises his attention , And the notices of the customs and traditions and systems and civilization of the different nations and peoples in this country that He lived with al - Marowzi.The Research Plan Research divided into preliminary and introduction, four chapters and a conclusion , The first chapter came in three sections , The first section took care of study of the life and geographic and doctor sharaf al - zaman tahir al - Marowzi , Such as his name and lineage scientific and upbringing and of his travels in the Arab Islamic countries , The second section we are believe in The influence of the age on the person of the author , So I dealt with the most prominent features of his time in the Seljuk state and its capital city of Mero, where he lived al - Marowzi , What characterized this era of conflict between the political forces that tried to seize power and the conduct of scientific movement , On the contrary, this contributed to the competition between scientific schools in multiple centers , The most important schools Baghdad, Cairo, Cordoba and Maro , In this study we have shown the flourishing of science and medicine in the Seljuk era. Then came the third section , Where we discussed the Arab - Islamic relations with the countries of India and China until the fall of the city of Baghdad by the Mongols in the year 656 AH , 1258 AD , Bypassing the country of Turk , To the fact that this relationship has come under the second section, which we introduced the first Seljuk state and growing up in the country's Turk and its extension after that to the other country,And its domination on the territory of the Abbasid state in the fifth century AH, the eleventh century AD In the second chapter , I thought that we should deal with the first section and briefly summarize the development of historical writing among Arab Muslims , And its early stages and its growth and development in the successive Arab and Islamic Arab countries until the era of al - Marowzi , And the stages in which it passed , From the style of biography and news , To the style of human being and the idea of the nation , Then we presented a second section on the geography of the Arabs , I presented the possession of the Arabs before to Islam geographical information astronomical benefited from them in their travels and movements , Then we showed the interest of Muslim Arabs in geographical knowledge, trips and trends , and The development of Arab - Islamic geographical authorship, from the keen attention of the Islamic regions to specialization in one country , And then the tendency to classify the geographical dictionaries influenced by the geography of other neighboring nations, especially the Greeks and Romans after the translation of the works of these countries , And in the third section dealt with the talk about the development of the emergence of animal science among Arab Muslims , Before the spread of the Islamic religion in the Arabian Peninsula and the development of this science during the successive Arab Islamic times , And the stages that passed until the era of Sharaf al - zaman taher Al - Marowzi and classification of the book of Tba'e Alhaywan. After learning about the development of the history, geography and animal sciences of Muslim Arabs, I had to learn in the third chapter the approach of Doctor Sharaf Al - Zaman Taher Al - Marowzi in writing the chapters of China, Turkey and India in his book Tba'e Alhaywan , And divided his sections into three paragraphs , presented in the first paragraph , The definition of the book , As it touched on the control of the title and proportion and date of composition , And the purpose of writing , Manuscripts , Typography , Arrangement and division and divided the investigation into three paragraphs , In the second paragraph, the basics of the organization and presentation of the historical article were presented in the three sections according to the sections , And the most important texts of rare historical documents presented by Al - Marowzi and unique in the chapter of China , In the third paragraph of this chapter, Al - Marowzi's method and methodology were presented in writing the three chapters and quotations from the ancient Greek heritage and Arab Islamic sources , Resources and elders , In addition to the style of criticism of the novels and the beginning of transport and end and the flags mentioned, all with live examples and resources Al - Marowzi within the three chapters - the study research. And to meet the title of this treatise right , I devoted the fourth and last chapter to talk about the cultural aspects in the countries of China, Turkey and India and was extrapolated from the total resources of Al - Marowzi in the three chapters , In which there are clear indications about the geographical aspects in the countries of China and Turkey and India and its locations and seas and cities and borders and how to manage this country and that in the first section , The second section has included Al - Marowzi resources for religious values and principles of ideological and social and cultural manifestations in the country of China, India and Turk , Finally, the third and final section was devoted to the economic aspects of this country , The research then concluded after that with a conclusion summarizing the research and its results , This was followed by a detailed outline of the basic sources and secondary references from which they were reported.Study of sources It is obvious to those who study the subject as the subject of this thesis , The researcher should not expect to find his scientific article in a wide range of ancient primary sources , because This type of studies and research , Have been shown only at very close intervals in comparison to other studies and research , Hence, the authoritative source on which this study is based is Al - Marowzi's own book (Tba'e Alhaywan) , And is the real source and solid foundation on which this study was built , It is from this book that information was extracted and conclusions drawn , And devising ideas , And building judgments and observations , Therefore, the reader will notice the frequency of the name of the book and its articles and chapters frequently , During the chapters and sections of this study
الملخص:
المصادر:

سياسة ايران الخارجية تجاه الاتحاد السوفيتي وبريطانيا 1965 - 1979 == Iran,s Foregn Policyu To Soviet Union And Britain 1965 - 1979

اسم المؤلف: علي جاري عليوي الجميعان
اسم المشرف: نعيم كريم عجيمي الشويلي
الموضوع العام: التاريخ
السنة: 2017
الموضوع الدقيق: التاريخ الحديث والمعاصر
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: ذي قار
الصفحات الاولى:
المستخلص: The study of Iranian foreign policy towards the countries of the Soviet Union and Britain, "1965 - 1979" of the important topics in contemporary Iranian history, we examined the nature of the foreign decisions Sketha Iran toward Amuqin large, the two political interests, economic and military from a long time ago, where every state has worked to maintain interests by every means available and is available at the stage of the cold war and I knew that Iran had become the most important squares as a result of its strategic location in the East and the West. Iran has worked to exploit the conflict between capitalism and ideas of socialism, I took Iran draws its foreign policy seriousness and warned severe, so as not to fall a political impasse with the European countries in support of Shah Mohammad Reza Pahlavi, Iran also has worked to exploit the historical stage for the construction of military and security institutions of the most advanced weapons in the stage British Soviet conflict, where he became the foreign policy of Iran's prominent role in European circles, especially in the global energy crisis and combat of ideas that do not want the big countries. Despite the successes of Iran's foreign policy in British circles, but she was born an ongoing internal problems as a result of Iran's drive to the outside on the aspirations of the Iranian people account who suffered political persecution under the power of the internal institutions that being the Shah to protect his property and follow - up work parties and internal organizations, arguing pro Alsovia. ontejh Union to employ Iran economic and political capacity for the benefit of its foreign policy was born of political and economic problems, the economic deficit, having dismissed Iran most of their budgets for the construction of the military establishment, was born of Iran's foreign policy, a wave of external criticism and internal, and this criticism prompted the major countries supporting to maintain influence and keep up with these new internal changes. Once the exterior was the Shah's support for the work of all the power of Aslal deteriorating internal situation as well as the face of the security establishment to hit the internal movements of political parties and religious institutions but to no avail, Vtjol support EU to continuous criticism has teamed up external and internal forces to overthrow the rule of Shah Mohammad Pahlavi, and the establishment of the Republic Iranian Islamist, have headed towards a new European countries in February 1979
الملخص:
المصادر:

المـؤسـسة العـسـكريـة اللبنانية وتطور دورها السياسي 1945 - 1976 == The Labanese Military Institution And The Development Of Its Political Role 1945 - 1976

اسم المؤلف: قاسم جباري لطيف المرشدي
اسم المشرف: صالح جعيول جويعد السراي
الموضوع العام: التاريخ
السنة: 2017
الموضوع الدقيق: التاريخ الحديث والمعاصر
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: ذي قار
الصفحات الاولى:
المستخلص: This dissertation entitled “The Lebanese Military Establishment and the Evolution of Its Political Role : 1945 - 1976” presents a study towards the emergence of military establishment and its evolutionary stages through which it went. It also demonstrates the power of military institution and the extent of its impact on the Lebanese political affairs, its stance towardspolitical development andevents on the Lebanese scene. Additionally, it demonstrates the military's position towards the Arab issues. Besides, this study touches on the army's position on the Civil War 1975 - 1976, and the impact of that war on disintegration and splitting of the army.As for the reason why we chose solely this topic, it is that there has been small number of those who have written on the topic, whether academics or authors, ie, the topic has not been investigated academically in a comprehensive and full study. It is also due to our desire to stand on the nature of the work of this institution that have shown a positive role in addressing most of political issues and events that took place in Lebanon during the period in question.The study began in 1945 as it represents the birth of Labanese military establishment afterLabanese Army units have moved from the powers of the French occupation to the authority of the Lebanese state with effect from the 1st of August 1945. This birth has formed a crucial stage of its kind in the history of this national institution.The year 1976 marked the end of the period in questionbecause that yearwitnessed the collapse of the Lebanese military in the wake of the outbreak of Civil War during which the army ended up with a state of disintegration and collapsein a way which seems to echo the rupture situation of the Labanese society after its officers and soldiers were involved in sectarian fighting.The nature of the study necessitated dividing it into an introduction, five chapters and a conclusion which contained the most important conclusions to which the study has reached. Chapter Onenecessitated studying the emergence and development of the Lebanese military institution 1916 - 1944. Although this chapter has come to clear the way for the study, it remains necessary for it highlighted the very beginning of the emergence of the Lebanese military formations under the French occupation. This chapter has been divided into into five Sections. Section I trackeddown the initial features of the Lebanese military formations under the French occupation. Section II dealt with the East Special forcesthat represented a new organization created by the French authorities. Section III was devoted to the study of the French - Lebanese treatyin 1936 and its impact on the military side. Section IVillustrated the implications and impact of the World War II on the Lebanese military formations, while Section Vdelved into the 1943 crisis and its impact on the formation of Bchamoungovernment and the National Guard teams.As for Chapter Two, it came up with a title, “The Lebanese Army and Its Role In Lebanese politics paths 1945 - 1952.” This chapter is one of the important chapters for it represents the formation phase of the military establishment and the beginning of a new phase towards building a national army. This chapter has been identified with four sections. Section Idealt with the French - Lebanese negotiations to hand over the army and bring withdrawal. Section II worked through the efforts of the Lebanese government to develop and maximize the armycapabilities, whileSection III highlighted the role of the Lebanese army in the 1984 Palestine War. Section IVdemonstrated the army position on the armed insurrection of the Nationalist Party of Syria 1949.Chapter Three traced down the Lebanese army position on the political developments during the period 1952 - 1958, a period that had proved the reliability and capability of the Lebanese army. The chapter has been divided into four sections. Section I dealt with the army's position the 1952 uprising, and how itremained neutral and preserved the public institutions of the state. Section II explained the disagreements between the army commander FouadShihab and President Camille Shamoun, and the reflection of this disagreement over the military institution. Section III focused on the army's position on the popular uprising in 1058, and how the army has managed to face its repercussions. Section IVfollowed up to trace down thesubsequent developments the uprising that led to US military intervention and the reactions of the Lebanese army.As for Chapter Four, it highlighted the emergence and growth of the Lebanese army and its explicit involvement in the various joints of the Labanese political life 1958 - 1971. In this chapter, we reviewed the most important developments witnessed by the military after the army commander (FouadShihab) assumed the the Republic presidency, and his reform efforts to modernize the military institution. Wecovered all this in Section I. As for Section II, it was a follow - op on the army's position on the military coup of the Nationalist Party of Syria in 1961. Section III focused on the emergence and growing role of the second office following the attempted coup and the army involvement in various joints of the Lebanese political life. In Section IV, we shed lights on the Shihabites’ failure in the 1970 elections of the Lebanese presidency, the arrival of Suleiman Franjieh to the presidency, and how the latter caused to dismantle the Second Office’s equipment and arrested and brought to trials senior military officers and leaders and the impact of all this on the reputation of the Lebanese militaryinstitution.Chapter Vbrought an end to this study by investigating the disintegration and collapse of the Lebanese military institution 1972 - 1976, which took place as a result of a host of factors, at the forefront of these factors was the Palestinian resistance and its non - compliance with the concluded agreements, a matter that weakened the military and contributed to the growth of armedmilitias. We made this clear in Section I. Section II was devoted to give a picture of the Lebanese army position on the events of the October war 1973. Section III tracked the position of the Lebanese army on theCivil War 1975 - 1976, and the corresponding calls to take the army to the streets and the charges that the army took sides with one component of the Lebanese people against another. Section IV highlightedthe disintegration and splitting of the army and the joining of its officers and soldiers for the fighting militias as they own weapons and equipment, each according to his loyalty and sectarian affiliation.The study reached a set of conclusions. Among them are that the Lebanese military establishment was keen since the beginning of its inception to adhere to the policies pursued by the political leaders, and has also taken a defending position for Arab issues including the Palestinian issue. It also took a neutral stance on internal issues and conflicts of which theuprisings of 1952 and 1958 was an example. Besides, the Lebanese army was not a coup - wired army nor did his leaders believe in the phenomenon of military coups; however,the coup attempt in 1961 has increased its intervention in politics. A series of events contributed to the undermining of the military status. Among those were the failure of the Shihabites in the presidential elections and the arrival of Suleiman Franjieh to the office who took down the Second Office and expulsedarmy senior officers and appointed instead new leaders on the basis of loyalties and sectarian lines. This led to the politicization of the army and undermining of its status. Besides, the existence of the Palestinian resistance and its lack of commitment to the agreements concluded with the Lebanese authorities led to the growth of armed militias that operate outside the authority of the state, a matter which led to the outbreak of the civil war that led in turn to the disintegration and splitting of the army. But nonetheless, the military establishment remainsa unifying factor for the Lebanese people with itsvarious denominations and sects. When it operates, it does so from within thisperspective, as an expression of one will and as a national demand.
الملخص:
المصادر:

الصراع البعثي - الشيوعي في العراق 1947 - 1968 == The Ba'Athist - Communist Conflict In Iraq 1947 - 1968

اسم المؤلف: مناف جاسب محمدعلي الخزاعي
اسم المشرف: مؤيد شاكر كاظم مخيلف الطائي
الموضوع العام: التاريخ
السنة: 2017
الموضوع الدقيق: التاريخ الحديث والمعاصر
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: ذي قار
الصفحات الاولى:
المستخلص: Iraq has witnessed through its modern and contemporary history the appearance of a lot of ideological and intellectual movements that were different and contradictory political parties, in their bid to gain power, authority and make political achievements , a collision and conflict emerged in trends ,ideas and goalsbetween those parties, the communist and the Arab Baath socialist.They are so contradictive ideologically with each other. Ones feels that the Arab Baath party foundation was the result of the existence of the Communist party and its ideas.This conflict was reflected on the political attitude in the Iraqi field and leads to a mass chaos and sever conflict which affected negatively the economic, social and political lives of the Iraqis.The statue of collision and conflict of both parties varies from time to another according to the weakness and strength state of the party.It started as a conflict in visions,Concepts and orientations then developed to political exclusions in most of times ,the conflict in all its forms was continues till 1968 when the Arab Baath party conquered the authority in Iraq and started to exclude and marginalization of all parties and political movements in Iraq.From this corner point came the importance of the subject that used to follow the conflict state between the two parties throughout the period of the study mentioned.We considered the period between ( 1947 - 1968 ) as the time limits.1947 represented the foundation of the Arab Baath socialist party when it was officially established on the 7th April 1947.While the 1968 represented the time of the Arab Baath controlling of all the authorities and power in Iraq then starting to cancel any role to the communist part on the Iraqi field as a real competitive partner,not only that but make it as a follower to him.During that period ,the two parties witnessed a noticeable ideological activity and a great expansion on their followers and supporting public and professional organizations.In addition to the multiplicity of their positions of internal economic, social and political issues.This study consists of a preface, five chapters and a conclusion and a set of appendices in addition to this introduction, which aimed to clarify the importance of the thesis material and identify the most important implications.In the Introduction, where we dealt with the beginning of the formation of ideas of both parties and the beginning of their establishment public principles.The first Chapter dealt with the intellectual contradictions between the two parties where we declared the intellectual differences between them within several aspects included internationalism , nationalism ,democracy , the Central public democracy , religion , scientific socialism , Arabic Socialism,Federal Union , immediate unity , the revolution , coup ,the situation concerning minorities and the rights of self - determination.The second chapter highlighted the theme of the relationship between the two parties against the internal political events for the period between 1947 - 1958 and included December prance 1948 ,the uprising in November 1952 , the United Popular Front in 1954 , the uprising of 1956 , the National Front Union in 1957 and wedeclared it clear that the attempt of each party's respective outcropping on the other party's account and try to prove their presence and their role in the events which led to compete and hostility between them.Chapter III wasdevoted to declare the case of conflict between the two parties in the field of interior politics ( July 14, 1958 - 8 in February 1963) which included the Arab unity and its impact on the conflict between them,the movements of Rashid Ali , Abdul WahabAl - Shawwaf and their impact on the relationship between the two parties as well as the assassination of Abdul Karim Qasim and its reflections on trying The relationship between them. This period had stormyevents in which conflict was very clear and reflected on all aspects of life causing total chaos and political confusion.Chapter IV devoted to declarethe case of conflict between the two parties on the leadership of the public professional and trade, labor union organizations and the competition work in different fronts.In addition the difference in their positions in dealing with the Kurdish issue to find the best solution.In chapter V the conflict had reached its climax point during the period (8 Feb.1963 - 17 July 1968 ) precisely in section one which lasted until 18 October 1963 when the first reign of Baath ruling had removed. During this period the shape of conflict characterized in various types; The elimination of political opponents, torture, arrest and severely treat political opponents. Then the conflict had shifted into sever ideological political differences at the end of Abdul - Salam Arif reign on 13th of April 1963.After that it changed into a difference in their visions and ways of solving the domestic problems.While the conclusion implemented the most important scientific results in accordance with their contents.We sought through supplements of this study to the publication of documents and important data to both parties with a difference because the Baath Party documents had been published in the book : "The struggle of the Baath documentary"This thesis depended on published and unpublished documentsAnd the literature of their members and diaries of followers who lived through the events and they were a center of the political decision at the time , the message and university thesis, which dealt with the march of the two parties and their activities and some English books, Arabic books, translated in addition to researches and published studies, newspapers, magazines and personal interviews with the Baathists and communists who had informed on events in that period.The study reached a number of conclusions which were represented that the establishment of the two parties was not derived out of total conviction is in their principles and methods but the foundation of the communist party came as the foundation of some Arab communist parties in Egypt and Algeria ,its ideology was not originally related to the people's needs and does not meet their political ,economic and social conditions. It was just an imported theory from the Soviet Union without any attention to the differences between both societies. On the other side ,the foundation of the Arab Baath socialist party came as a re - action on the foundation of the Communist party. Also its principles and views Was not taken from the Arabic society but was a combination of a scattered ideas from all over the world ;French socialism ,German Nationalism , some of the Islamic tradition and a lot of Marxism thoughts. They are a mixture of not harmonized thoughts.We have proved the ideological conflict between the two parties which taken some contradictive decisions and showed that the Baath party has no obvious ideology but just to oppose the communist party although on the surface they seem to meet ideologically in dealing with some of the interior problems during the period 1974 - 1958 but the differences sooner began to appear after each event in deciding the slogans the demonstrators should pear and the role of each party in controlling the street. Both of them assumed to control the streetand this leads to the enlargement of the difference between them characterized in the speeches. As a result the conflict became stronger.During the period1958 - 1963 the conflict decreased and shifted into coups and conspiracies.The Baath was the generator of these events aiming to get rid of the communist party to control the country and ruling it, Baath realized the only way to defeat the communist party was through assassinatingAbdul Kareem Kasim.This plan was targeted towards the communist party and that what was happened on the 8th of Feb. 1963.Concerning the working on various fronts ,both parties was not truthful in his preaches but they would like to increase their followers and to capture the authority and power at the same time to destroy all the political opponents.Their invitations to solve the Kurdish issue were not stable and fundamental they vary according to the strength and weakness of each party during the period he passed through. Conflict increased and characterized in different types after 8th Feb 1963 the Baath After the successful coup and began to rule Iraq.The first thing Baath started with is to get rid of all his political opponents from the communist party severely. That was not stopped at preventing communist ideology from being spread but included the assassinations of communist members and arresting some of them ,torturing them and denies them. The conflictviolence released after the end of Baath ruling on the 18th November 1963 and changed into a differences in visions and concepts concerning internal political situations and this was continues until the return of the Baath to the ruling of Iraq on the 17th of July 1968.
الملخص:
المصادر:

الطبرسي (ت548هـ) واثره في تدوين السيرة النبوية : دراسة في منهجه ومورده == The Effect.Of Al Tubrasi (548H) In Notation The Biography Of Prophet Mohammed : A Study In His Process And Resources

اسم المؤلف: سلوى حسن عيدان الحسناوي
اسم المشرف: هاشم ناصر حسين الكعبي
الموضوع العام: التاريخ
السنة: 2017
الموضوع الدقيق: التاريخ الاسلامي
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: Al Tebrisi is an author of the fifthly century who took much care with conveying historical events, in particular, the prophet's biography ( Seira). He had an explicit method in conveying the prophet's biography in all science fields when he classified and not only in history. That means, transferring them into interpretation books through explaining the Quranic Aya or through clarifying reasons behind descending the Quranic ayas, beside philology books that imply the prophet's biography (peace be upon him ). Through mentioning the philological issues, he talked about the historical accidents that relate to the prophet's life( peace be upon him ). He devoted some historical books to the prophet's biography and conveyed whatever relate to the prophet's life( peace be upon him )starting from his affinity to his death. In conveying the prophet's biography, he depended on several references which were divided into two groups. The first group includes the explicit such as the narrators and books. The second group includes the implicit which were mentioned as vocabularies, some of these like (it was mentioned by, it was said, …) and other items that was mentioned without a narrator or a book that refer to knowing the resource. The study was divided into three chapters preceded by introduction and followed by conclusion. The first chapter was entitled (Al Tebrisi's era, life, and the scientific status). It has three sections. The first section was entitled Al Tebrisi's era. The second dealt with his life. The third was about his scientific status. The second chapter tackled the study (Al Tebrisi's resources of the prophet's biography. It has three sections. The first was entitled Al Tebrisi's reasons in the prophet's biography in the two books (Ilam Al Wara Be Ilam We Taj Al Muwaleed ). The second dealt with Al Tebrisi's resources of the prophet's biography in books of interpretation (Isbab Al Nezoul, Mejmah Al Beyan Fe Tefseir Al Quran, and Tefseir Jewamih Al Jamih ). The third mentioned Al Tebrisi's reasons in the prophet's biography in the two books of philology (Al Adab Al Deneyh and Al Muitelif Min Al Mukhtelif bein Iemet Al Selef ). The third chapter was devoted to study (Al Tebrisi's approach in the prophet's biography. It has three sections. The first dealt with Al Tebrisi's approach in the prophet's biography in the two books (Ilam Al Wara we taj Al Muwleed ). The second dealt with Al Tebrisi's approach in the prophet's biography in the interpretation books (Isbab Al Nezoul, Mejmah Al Beyan Fe Tefseir Al Quran,and Jewamih Al Jamih ). The third mentioned Al Tebrisi's approach in the prophet's biography in the two books (Al Adab Al Deneyh and Al Muitelif Min Al Mukhtelif bein Iemet Al Selef
الملخص:
المصادر:
1 ... 38 39 40 41 42 ... 47