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دراسة بكتيرية ووراثية لبكتريا Gardnerella vaginalis وعلاقتها بالولادة المبكرة == Bacteriological and Molecular study of Gardnerella vaginalis association with preterm labor
Author name:
ازل علاء عباس الربيعي
Supervisor name:
الهام عباس بنيان الساعدي | لميس عبد الرزاق عبد اللطيف
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Doctorate
University:
University of Babylon - Faculty Of Medicine
Language:
English
University location:
Babylon
First pages:
19T1259 - p.pdf
Abstract:
In this study , one hundred fifty samples were collected from paitents with preterm labor (PTL), who have been attending to Babylon Maternity and pediatric hospital and Al - Hilla Teaching Hospital, at the period from February to October 2016. Two swabs were collected one for culturing and the other for direct extraction for isolation Gardnerella vaginalis.Out of the 150 samples only 6(4%) on culture and 30 (20%) on molecular level isolated from preterm labor caused by Bacterial vaginosis , Urinary tract infection and aborted women. The results isolation and laboratory diagnosis as well as biochemical test approved that there is only 6 isolates belong to G.vaginalis confirmed by using Vitek 2 system and molecular detection by specific primers.Preterm birth is one of the most common causes of neonatal morbidity and mortality. Associated with sub sequent preterm labor in up to 40% of cases as shown in our resultsThe isolates are varied in their ability to produce sidrophore and extracellular protease in addition to their hemolytic activity .Adherence activity were investigated and indicate that G.vaginals have pili on her surface help her to attached and initiated infection Metronidazole was effective in prevent adherence at low concentration (5%) was 100% percentage.All isolates are able to produce biofilm in different level which provided as main step in pathogenesis caused it can evade and evoke the action of immune response and antibiotics , Alum , sodium chloride and sodium bicarbonate were shown very high effectively to eradicationSummaryIIG.vaginalis biofilm which gave results (0%) whereas the thymol extract (carvacrol) were moderate in action gave (50%) effect on Biofilm .Results also preveld that increased of pH value give a chance to overgrowth of G.vaginalis .The results indicated that Lactic acid considered as a limiting factors preventing growth of G.vaginalis when its grown with lactic acid , measured by optical Density (OD) the high production level is (1.901) in 2% concentration Whereas the lowest level is (0.32) in 10% concentration.Isolates Have ability to produce wide spectrum Bacteriocin which inhibited growth of both Gram positive and Gram negative bacteria that founded in vagina environment, Staphylococcus saprophyticus were the most bacteria that inhibited .extracted Vaginolysin was have a completed hemolytic activity in different temperatures (37 - 38 - 40 - 42). The results showed that its activity as were increased in high temperature.Recent study were clarified that purified vaginolysin was active in presence of cholesterol in media, gave (1.205) growth rate measured by the optical density at (100%) concentration. vaginolysin is cholesterol - dependent cytolysin (CDCs) toxin.Antimicrobial activity were investigated to the extracted Vaginolysin to evaluation of its activity depending on inhibition zone, the results showed that candida is more affective between the other microorganism gave (1.3)mm.Some virulence factor have been detected in molecular level ( Vly - SI - LuxS - LexA - rec a - Pl - Fur - Omp - Gal - protease ). Which wereSummaryIIIvaried in production may be as a results to the un presence of this gene in bacteria , may be silent gene or due to the amount of bacteria in sample are rare.Three Antibiotics were used at a molecular level (Tet b) were varied between resistance and sensitive to this gene (10) sample are resist,(RdxA) all sample are sensitive to its gene at percentage (100%), whereas (erna)gene were resist in all samples (100%).The results show there was no sequence homology between Mycoplasma spp. and G. vaginalis in the adhesion level this were detected by P30/P32 specific primer .The outcome of RFLPS - PCR is confirmed that G.vaginalis is virulent and have a role in preterm labor by degradation of membranes with the association of variable Silidase and presence of β - Galactosidase .MBL (mannose binding lectin ) levels were have a direct role pregnancy. It is also apparent that MBL plays a role in the defense against vaginal infection. MBL is essential to successful pregnancy in regards to maternal defense against infection, recent study indicate that MBL deficiency related to pregnancy outcome disorderedthis considered the first study of mannose in Iraq .DNA sequencing were done for two main virulence factor Phospholipase gene and Quorum sensing gene which showed some variation , then recorded in NCBI - gene sequencing as first descriped in Iraq.
