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تقييم الطفرات الوراثية ل BRAF, IDH1 وفقدان 1p/19q في الاورام الدبقية للمرضى القراقيين : دراسة نسيجية مناعية كيميائية مع التهجين الموضعي المتفلور == Assessment of BRAF, IDH1 mutations and 1p/19q loss in Gliomas in Iraqi patients. Immunohistochemical and Fluorescence in Situ Hybridization study
Author name:
زهراء مروان شعبان العمر
Supervisor name:
سالم رشيد حمودي العبيدي
General topic:
Medicine
Specific topic:
Diseases - Genetics
Degree:
Doctorate
University:
University of Baghdad - Faculty Of Medicine
Language:
English
University location:
Baghdad
First pages:
19T1427 - p.pdf
Abstract:
Background : - Gliomas account for almost 80% of primary malignant brain tumors, so, they considered the most common primary malignant brain tumors in adults. The major types of glial tumors are astrocytomas, oligodendrogliomas, and ependymomas. Isocitrate dehydrogenases 1, BRAFV600E and 1p/19q co - deletion are important molecular markers nowadays for diagnosis and prognosis of gliomas. Isocitrate dehydrogenase mutation might be encounter in the low - grade glioma, occurs in early stages of development and directs the progression of the tumor to a higher grade. BRAFV600E is a point mutation, resulting in a valine to glutamic acid substitution at position 600 and this mutation occurs more in pediatric gliomas but less frequent in adult gliomas. Co - deletion of 1p/19q results from a non - balanced translocation t (1 : 19) (q10 : p10) with subsequent loss of one of the derivative chromosomes and highly associated with oligodendroglial tumors morphology and improved survival.Aim of the study : - To assess the frequency of Isocitrate dehydrogenases 1 and BRAF mutations in Iraqi patients with gliomas by immunohistochemical study, to correlate their immunoreactivity with some clinicopathological parameters. To validate frequency of 1p/19q loss by Fluorescence in Situ Hybridization study and correlate the deletion with the some parameters.Patients and Methods : Formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in Baghdad city were collected in this retrospective selective study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, that is manual tissue microarray, in which twenty - four small cores (each measure 2mm) of represented tissue made, then cut by microtome. IHC detection of Isocitrate dehydrogenases 1 and BRAFV600E antibodies did by Dako autostainer link 48. Assessment of co - deletion of 1p/19qAbstractIII
