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تقييم قابلية بعض انزيمات السليليز الفطري على تخمير تخمير بقايا النباتات لانتاج الايثانول == Evaluation of Capability of Some Fungal Cellulase In Fermentation of Plant Residues For Ethanol Production

Author name: عذراء حرجان محسن الدحيدحاوي
Supervisor name: فاطمة عبد الحسين التميمي | محسن هاشم رسن
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية الى تقييم قابلية بعض انزيمات السليليز الفطري على تخمير بقايا النباتات على انتاج الايثانول من بعض الفطريات المحلية المعزولة من 50 نموذج من التربة والذي تضمن Aspergillus oryzae , A. niger, A. terreus , A.flavus , A.fumigatus, A.parasiti | The present study was conducted to proceed a Bioethanol production using some of the waste fermentation plant by cellulosic hydrolysis enzyme produced by some local fungi isolated from 50 samples from soil which were including Aspergillus oryzae, A.niger, A.terreus , A.flavus , A.fumigatus, A.parasiticus, A.nidulans, Penicilliuum chrysogenum, Trichoderma longi, Rhizopus stolonifer, Cladosporium spp, Mucor indicus, Trichothecium spp. The prominent isolates were A. oryzae, A.niger, A. terreus which were chosen for further studies and screening to producing cellulase enzymes the fungal isolates revealed variation values of clear zone It’s (5.1, 5, 5, 4.8) cm to A. oryzae, A.niger, A. terreus , A.flavus respectively. The results revealed that the suitable carbon source to the cellulase activity was induced in different raw plant substrate, the highest was produced when using Corn cobs, Rice husk and Reed reached to (1.72, 2, 1.26)IU/ ml respectively when using A. oryzae and (1.24, 1.17, 1.89) IU/ ml when using A.niger, and (1.31, 1.19, 1.93)IU/ ml when using A. terreus. but using the cellulose powder and CMC, Avical sole carbon source total cellulase given least activity compare with raw plant substrate.on the other hand, using Mandels - Weber medium activity of enzyme production by A. oryzae, A.niger, A. terreus recorded (6.51, 4.14, 5.61) U/ ml respectively when using Corn cobs and (5.79, 4.03, 5.24) U/ ml and (5.55, 3.97, 5.19) U/ ml when using Rice husk and Reed by different fungi. The results refers that , the best chemical pretreatment results were appear when using NaOH at 100Co in 30min on Corn cobs, the total cellulase activity were (1.63, 1, 1.21) U / ml when using fungal isolated to degradation after treatment, while using hot water at100Co without NaOH in 15 min to pretreatment Rice husk activity were (2.16, 2.07, 2.12) U / ml but Reed are appear high activity when pretreatment with NaOH at 100C0 in 15min activity It’s (2.28, 1.65, 2.09) U / ml. The results revealed that, The optimized conditions of the enzymes were different in this study that give activities of Fpase, CMCase and ? - glucosidases to fungus A. oryzae compared to the rest of the fungi using Corn cobs as carbon source, it reached to(2.21, 2.30, 46.72) IU / ml respectively when pH6, and temperature tested for these enzymes recorded (1.64, 1.61, 30.86) IU\ ml respectively at a temperature of 30 C° and when using the concentration of substrate gave activity (2.4o, 2.48, 42.34) IU / ml of 6 % from Corn cobs and recorded when using nitrogen source concentration at 6 % (1.92, 1.89) IU\ ml in KNO3 and (NH4)2SO4 to Fpase and CMCase and the effectiveness of ? - glucosidase at the same nitrogen source (43.54, 50) IU / ml. on the other hand, showed enzymes Fpase, CMCase and ? - glucosidase high activities to ? - glucosidase, CMCase to fungus A. oryzae compared to the rest of the fungi using Reed as carbon source reached (2.31, 2.21, 46.15) IU/ml respectively at pH 6, and at different temperatures were (1.66, 30.94) IU/ml to Fpase and ? - glucosidase at 30 C°, but CMCase gave the highest efficacy (1.60) IU \ml at 25 C° and decreased effectiveness to (2.60, 2.57, 49.69) IU / ml at 6 % of the carbon source recorded effectiveness of the enzyme Fpase (1.19, 1.09) IU / ml when using (NH4)2PO4 and (NH4)2SO4 at 6 % as a nitrogen source of from either enzymatically CMCase and ? - glucosidase was effective at same nitrogen source (1.29, 2) and (35.19, 40.41) IU / ml respectively.The results showed when you use a Rice husk recorded higher effective enzymatic Fpase and CMCase and ? - glucosidases of fungus A. oryzae was (2.32, 39.27) IU /ml for Fpase and ? - glucosidases at pH 6 but CMCase gave the highest efficacy at 5 pH as recorded (2.04) IU / ml at temperatures 30 C°, reached to (1.64, 1.58, 29.13) IU / ml respectively for Fpase and CMCase and ? - glucosidases at a temperature of 30 C°, and when using 6% of the substrate was effective (2.47, 2.19, 44.86) IU / ml, activity recorded when using (NH4)2PO4 and (NH4)2SO4 at 6 % as a nitrogen source of (1.1) IU / ml to FPase on either enzymatically CMCase and ? - glucosidase was effective when use same a nitrogen source (1.18, 1.83) and (31.92, 36.91) IU/ml, respectively. In addition, The results refers when precipitation by ammonium sulfate specific activity were (38.69, 1.25, 5767.5) IU of the enzyme with 0.012 mg protein and then, Elution protein of crude enzyme solution of A.oryzae from DEAE - cellulose column was shown to the protein was separated in (33) fractions.It was found that only the fractions (F - 8) contained cellulase activity, while fraction (F - 30) eluted in column containing buffer and different concentrations of NaCl then using gel filtration and gave three peak of enzymes alone and Characterize enzymes purified FPase activity was found at 5.5 pH which (0.91) U and temperature was having activity at 25 and 30 C°, were (0.14, 0.15) U and CMCase found that the enzyme exhibited maximum activity at 25 C° was (0.33) U but 30 C° for ? - glucosidase was (38.22) U and the molecular weight of the protein was found to be about (38, 52 and 49) kD for (FPase, CMCase, ? - glucosidase) respectively. Finally, estimated ethanol density (0.80 - 0.91) g /ml and ethanol concentration (60 - 97) % but using chromic acid test of purified ethanol the complex enzyme with Reed, complex enzyme with Corn cobs recoded high value of ethanol which were (0.98, 1.72, 1.87, 1.89 ) % and (1.09, 1.83, 1.92, 1.99) % respectively but complex enzyme with Rice husk were given (1.01, 1.12, 1.48, 1.79) % in 4, 5, 6, and 7 days respectively on the other hand, when using the A.oryzae and A.terreus with Reed gave high ethanol value from other fungi with substrate in this study, and observed Rice husk in all results was recorded less absorbance at 350 nm and determination of ethanol concentration by gas chromatography mass used ethanol concentration 99 % as standard, also we showed the ethanol when using enzyme pure with substrate in which were (93.7, 97.1, 88) % ethanol to enzyme with Corn cobs and Reed and Rice husk respectively

دور بكتريا الزوائف. Pseudomonas spp في التحلل البايلوجي للمركبات الهيدروكاربونية والمقاومة الاحيائية == The Role of The Pseudomonas Spp In The Biodegradation of The Hydrocarbon Compounds And Biological Resistance

Author name: عقيل شنان عمران الميالي
Supervisor name: علي عبد رحيم الناشي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: شملت الدراسة جمع 60 عينة من مصادر بيئية مختلفة ضمت ترب ملوثة وغير ملوثة بالمركبات الهايدروكاربونية ومياه بزل في محافظة الديوانية ومن مصادر سريرية لمرضى اخماج المسالك البولية في مستشفى الديوانية التعليمي, للمدة من 28 كانون الثاني2013 حتى 1 شباط 2014. هدفت | This study included collection of 60 samples from different environmental sources involved contaminated and non contaminated soils by hydrocarbon compounds and from drainage waters in Diwaniyah province and clinical sources in Diwaniyah teaching hospital, for the period from 28 January 2013 to 1 February 2014. This study aimed to isolation and identification the species of Pseudomonas and metabolic efficiency test in the analysis of petroleum hydrocarbon compounds and measure the effectiveness of antagonism in resistant pathogenic fungi to tomato plant. Thirty four isolates were identified, the Pseudomonas spp. was the highest frequency with 22 isolates. These isolates distributed to 16 isolates of pseudomonas aeruginosa and 6 isolates of pseudomonas fluorescens. Also this study included isolation of two pathogenic fungi which were Alternaria alternate and Fusarium solani in tomato plant. Statistical analysis revealed significant differences at (p<0.05) between the bacterial isolates in their analysis of hydrocarbon compounds, when the optical density measurement as an indicator of the effectiveness of growth on hydrocarbon sources. All isolates showed different optical densities were highest isolation of pseudomonas aeruginosa isolated from urinary tract infections as optical density amounting to 0.23nm. Also showed an increase in electrical conductivity values for all isolates within the incubation period, the highest value to the hospital (1) isolate with 12.8 mc/cm in the third week of the incubation, But decreased in the sixth week to 8.7 mc/cm and all isolates showed a good effectiveness of electrical conductivity in the third week and noted significant differences (p<0.05) in electrical conductivity values for isolates compared with control of 7.2 mc/cm. The results of the DNA electrophoresis of the all isolates contain the two genes (C23O and rhIB) which they code for the production of hydrocarbon - degrading enzymes. In Bio - antagonism experiments noted that all isolates were inhibitory to the growth of two pathogenic fungi and stimulating the growth of tomato plants in glass and field experiments through increase germination, vegetative ratio and root length and increased weights with significant difference at (p<0.05)with seed treatment with fungus only. The best isolates increased in germination were pseudomonas aeruginosa hospital isolate and pseudomonas fluorescens generating isolate which had stimulated germination rate to 76% with significant difference when compared with the control of Alternaria alternata and Fusarium solani which germination rate was 36 and 40 respectively. Pseudomonas aeruginosa which isolated from soil was more efficient in increasing the length of the stem, the stem height was 7.53 cm compared with the control treatment with F. solani, the stem height was 5.7 cm. Pseudomonas aeruginosa hospital which led to an increase in the length of the total root to 3.66 cm compared with the control treatment with F.solani at 1.53 cm. The increase in fresh and dry weights of Total vegetative reached 0.64 gm and 0.065 gm respectively when the treatment of seed contaminated with F.solani fungus and pseudomonas aeruginosa hospital isolate compared to the control which the weights were 0.11 g and 0.011 gm respectively. The highest increase in fresh and dry weights of total root of 0.057 gm and 0.0072 gm respectively when treated seeds contaminated with the F. solani with P. fluorescens hydrocarbon contaminated soil isolate compared with control that the fresh and dry weights was 0.032 g and 0.0017 g respectively. The highest value of stem length registered when treating seed with A. alternata which was 7.5 cm when treating seed contaminated with A. alternata by P. fluorescens isolated from soil contaminated with hydrocarbon compounds. The lowest value of stem length was 7.3 cm for the treatment of seeds contaminated with the A. alternata by P. aerugionsa isolated from the drainage water compared with control treatment (seeds contaminated with A. alternata) which stem length was 6.33cm. As for the fresh and dry weight for total vegetation was the highest value of 0.8 gm and 0.793 gm respectively when the treatment of seeds contaminated with the A. alternata P. aerugionsa isolated from urinary tract infections and the lowest value of fresh weight was 0.61gm for total vegetation when treated seeds contaminated with the A. alternata by P. aerugionsa isolated from soil non contaminated with hydrocarbon compounds and 0.069 gm when treated seeds contaminated with the A. alternata by P. aerugionsa which Isolated from drainage water compared with control treatment which were 0.15 gm and 0.0014 gm of fresh and dry weight for total vegetation, respectively. As for fresh and dry weight of total root were the highest value 0.071 gm and 0.008 gm respectively, when the treatment of seeds contaminated with the A. alternata by P. fluorescens isolated from soil contaminated with hydrocarbon compounds. while the lowest value were 0.058 gm and 0.0065 gm when treated seeds contaminated with the A. alternata by P. aerugionsa isolated from soil non contaminated with hydrocarbon compounds compared to control treatment (seeds contaminated with the A. alternata only)which the fresh and dry weight values for total root were 0.046 gm and 0.0018 gm respectively. When antagonism study in the glass field, the highest percentage of inhibition of the growth of the F. solani on PDA and cetramide agar were 42% and 61% respectively, when treated with P. fluorescens generator isolate. While the bacterial antagonism with A. alternata on PDA media, the highest percentage of inhibition 51% when treated with the transaction P. fluorescens generator isolate on PDA media. Generally, the treatment of pathogenic fungi with biological control bacteria had reduced the percentage of infection and increase the percentage of germination.

دراسة مناعية ووراثية تتبعية لمرضى العقم من الرجال == A Prospective Immunological And Genetic Study of Infertile Men

Author name: علي عبد الزهرة مهدي الفحام
Supervisor name: يحيى كاظم السلطاني | عبد الزهرة كاظم محمد علي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: ان من الحقائق العلمية الثابتة اليوم ان الفحص الروتيني للسائل المنوي يعد عاملا تنبؤيا اجماليا للقدرة الاخصابية للرجال، ولذلك فان حاجة متزايدة بدات تبرز لاكتشاف فحوصات وظيفية جديدة في عملية تقييم العقم عند الرجال. ان الهدف الاساس من هذه الدراسة هو تعزيز الج | It is a scientific fact today that routine seminal fluid analysis is a key predictor of male reproductive potentiality ; so that there is an increasing need for finding out other functional tests in the assessment of male infertility. The main goal of the current study is to consolidate the scientific and practical efforts concerned with male infertility assessment especially from immunological and genetic aspects. The study was carried out between January 2013 and December 2013 including one hundred (100) selected infertile men who attended the Fertility Center in al - Sadr Medical City in Al - Najaf Al - Ashraf Governorate, the study also included twenty (20) healthy volunteer fertile men as a control. Serum and seminal antisperm antibodies (ASA) were determined by ELISA (Enzyme Linked ImmunoSorbent Assay) technique ; sperm chromatin condensation was evaluated by aniline blue staining (AB) ; sperm DNA fragmentation (damage) was evaluated by toluidine blue staining (TB). The results showed that the incidence and concentration of serum and seminal plasma ASA in the infertile men were significantly (p<0.05) higher than that in control fertile men. The incidence and concentration of serum and seminal ASA were also significantly (p<0.05) higher in normozoospermic infertile patients than that in control fertile men. There was a high significant negative correlation (p<0.01) between the concentration of serum and seminal plasma ASA in the infertile men and sperm motility and progressive motility, the concentration of serum and seminal ASA also showed a high significant positive correlation (p<0.01) with sperm agglutination, and a significant positive correlation (p<0.05) with seminal WBC count. The results also revealed that there were no significant effects (p>0.05) for patients' age, infertility duration, ABO blood groups and smoking habits on the levels of ASA in the serum and seminal fluid, while higher significant increase (p<0.01) in serum and seminal ASA concentrations was observed in infertile patients with varicocele as compared with those without. The results have also revealed that the percentage of sperm with chromatin decondensation and DNA damage has shown a high significant (p<0.01) increase in infertile patients compared to fertile men. There was also a significant increase (p<0.05) in sperm DNA damage in normozoospermic infertile patients comparing with fertile men, but no significant difference (p>0.05) was found in sperm chromatin condensation between normozoospermic patients and fertile men. Both sperm chromatin decondensation and sperm DNA damage showed a significant positive correlation (p<0.05) with sperm morphology, and a significant negative correlation (p<0.05) with sperm concentration, motility and progressive motility. The present study also revealed that the level of chromatin decondensation has been affected by patients' age, infertility duration, smoking habits and varicocele. It was observed that the higher percentage of chromatin decondensation was recorded in patients older than 37 years, and in patients subgroups with infertility duration more than 15 years, it was also higher in smoker as compared with non - smoker patients and in those with varicocele as compared with those without. Similarly, the level of sperm DNA damage has also been affected by patients' age, smoking habits and varicocele. It was observed that the highest percentage of sperm DNA damage was recorded in patients older than 37 years. The highest level of sperm DNA damage was also seen in smoker patients as compared with non - smokers and in those with varicocele as compared with those without. In contrast, the effect of infertility duration on sperm DNA damage was not statistically different (p>0.05). The correlation and regression results recorded high significant correlations (p<0.01) between serum ASA and seminal plasma ASA, and between sperm chromatin decondensation and sperm DNA damage. However, there was no significant correlation (p>0.05) between the level of serum and seminal ASA and each of sperm chromatin decondensation and sperm DNA damage. The current study concluded that the defects in sperm chromatin status (chromatin decondensation and DNA damage), and the immunological disorders caused by serum and seminal plasma ASA may - at least partially - contribute to the etiology of infertility of the patients under study, even in those with normal seminal parameters. However, it seems that ASA affect fertility in a pathway that is different from that affected by sperm chromatin defect. It was recommended that both sperm chromatin staining techniques and ASA tests could be routinely used as complementary tests to diagnose infertility.

تشخيص بكتريا Neisseria gonorrhoeae بالطرق التقليدية والجزيئية في المرضى الذكور ودراسة مدى تاثيرها في حدوث الحذوف في موقع AZF == Conventional And Molecular Diagnosis of Neisseria Gonorrhoeae In Male Patients And Study Its Suspected Effects In Microdeletions In Azf Locus

Author name: غزوان علي مسلم الرماحي
Supervisor name: عبد الرضا عبد الحسن اللامي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: This study includes 82 clinical samples from male patients suspected to have gonorrhea (urethral discharge with dysuria). Two types of samples were collected from each patient, urethra swab and blood samples from patients who attended Al - Yarmouk teaching hospital in Baghdad city, Clinics and private laboratories during a period from December 2012 to April 2013. All of them were married and have children's, and 20 samples were taken from apparently healthy subjects.Cases caused by gonococci were diagnosed by finding the Neisseria gonorrhoeae bacteria in the samples using microscopic examination and culturing on rich media chocolate agare for growth bacteria and selective media modified Thayer martin agar (MTM) contact antibiotic to prevent growth of all the types of bacteria except N. gonorrhoeae.Microscopic examination using specific kit for gram stain, showed gram negative diplococcus, like bean shape. Culturing on rich media revealed that 82 (100%) samples positive but re culturing on Modified Thayer martin media revealed that 76 (92.68%) out of 82 positive. Then biochemical test had the same results.Results of the molecular diagnosis of the bacteria causing gonorrhea using specific primers that were specific for the Orf1 gene, revealed that 80.26% of samples (61 out of 76) were positive. Results of the microdeletion in Y chromosome AZFc region revealed that no microdeletion were occurred in SY - 254 STS and BPY - 2 gene.This study, provided high specificity and sensitivity for the diagnosis of gonorrhea using PCR technique which is cheaper and faster than traditional methods. Also, PCR - based method for detection of N.gonorrhoeae can be readily used in hospitals and laboratories.

الكشف عن السمية الوراثية لمستخلص نبات الصبار Aloe vera باستعمال المؤشرات الجزيئية والخلوية == Genotoxic Effect of Aloe Vera Extract Using Molecular And Cytogenetic Markers

Author name: غيث منذر فاضل
Supervisor name: نضال نعمة حسين
General topic: Biology
Specific topic: Plant - Genetics
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: نبات الصبار Aloe vera L. من النباتات المهمة والتي تستعمل حاليا بشكل واسع في مجال صناعة الادوية ويضاف هلام الصبار الى اغلب مستحضرات التجميل مع بعض المواد الغذائية في جميع انحاء العالم, لذا تم دراسة التاثيرات السمية الخلوية والوراثية لتراكيز مختلفة من المس | Aloe vera L. is one of the important plants in the world.It is currently and widely used in pharmaceutical industry, and its gel in some time added to some foods all over the world. Therefore, the present study was conducted to evaluate the cytotoxic and genetic effects of crude, alcohol and aqueous extracts of Aloe vera gel toward the onion(Allium cepa L.) roots (as a biological system) exposure at different periods. Onions roots were treated with different concentrations of the extracts : 2% , 5%, 10%, 20% and 40% (v/v)of the crud extract , 5%, 10%, 20% 30% and 50% (v/w) of alcohol extract and the concentration 25%, 50%, 100%, 150% and 200%(v/w) of aqueous extract of Aloe vera gel exposed for different period (24, 48 and 72) hour. The investigation were focused on the effect of these extracts on onions roots length rate and some cellular properties (mitotic index, phase index, the percentage and type of chromosome aberrations, as well as a genetic study using Random Amplified Polymorphic DNA (RAPD) of onions roots treated with different concentration of this extracts. Results showed that all Aloe vera gel extracts had inhibit activity toward onion roots length rate. The inhibition associated positively with the increasing concentration. The effective concentration (EC50%) were 10% of the crude extract, 20% of the alcoholic extract and 100% of aqueous extract, indicating the effectiveness of the crude extract followed by alcoholic extract and then the aqueous extract. Cytological study showed that all Aloe vera gel extract revealed a significant decrease in mitotic index (MI%) of the onion roots cells compared with the control group, and the effect was positively associated with the increasing concentrations of the extract. However, the effect was independent on the exposure period. The concentrations 10%, 20% and 150% of the crude, alcohol and aqueous extract respectively indicated a reduction of the mitotic index to almost 50% of the control treatment. Therefore, this concentrations considered as sub - lethal concentrations for this extracts respectively while the higher concentrations than 30%, 50% and 200% of the crude, alcohol and aqueous extract which reduced the mitotic index to almost 22% of the control treatment were considered as lethal concentrations. The results also showed a significant decreases in prophase and an increase in metaphase in onion roots cells treated with extracts comparison with control treatment. Many of chromosomal abnormalities were appeared due to the treatments of the onion roots with different concentration of the extracts, the percent abnormalities was increased with the increasing concentration of the extract and exposure period. The most frequent of chromosome abnormalities were chromosomal stickiness, disturbed chromosome, chromosomal bridges, C - mitosis, vagrant chromosomes in addition to the other chromosomal abnormalities star telophase. The toxicity effect of Aloe vera gel were studded on molecular level by using random amplification polymorphism of DNA(RAPD). Ten random primers were used, seven of them gave polymorphic bands in all the studied samples and their molecular weights were ranging from 100 to 1600 base pair. The results of Random Amplified Polymorphic DNA (RAPD) showed significant difference in the number of DNA band and their molecular weight of the onion roots treated with Aloe vera extracts compare to control treatment. The high concentrations(higher than EC50%) of extracts were showed to be more effective on the DNA through the appearance or disappearance of larger number of DNA band in comparison with control. The genetic template stability (% GTS) was significantly decreased in DNA of onions root treated with high concentrations (higher than EC50%) of Aloe vera extracts, therefore the high concentrations of Aloe Vera extracts were considered to be genetically toxic. The genetic dendrogram was obtained using Jaccard factor of genetic divergence and the result showed segregation of the samples treated with heights concentrations (%40 200% and 50%) of crude, alcohol and aqueous extract from other samples and control treatment. Therefore, the RAPD method can be considered as an efficient method in detecting for assessment of genotoxicity of crude, alcohol and aqueous extract of Aloe vera gel.

تقييم وعزل فيروس الانفلونزا للانسان ودراسة الاستجابة المناعية في الجرذان التجريبية == Evaluation And Isolation of Human Influenza Virus And Studying Their Immune Response In Experimental Rats

Author name: فاديه مهدي مسلم العبيدي
Supervisor name: عبد المجيد عبد الله السعدي | يونس عبد الرضا الخفاجي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: This study has been conducted for the first time in Najaf / Iraq, and the study included the preparation of a local inactivated vaccine (whole and subunit).The number of cases infected with seasonal influenza virus were 647 cases out of one thousand suspected case. They were distributed into eleven groups. Seasonal influenza virus was detected by two diagnostic methods (rapid test device and real time PCR).The present study has reflected that the most diagnosed cases infected with seasonal influenza virus during the period extended from March 26, 2012 up to April 30, 2013 represented by were type A (H3N2) (283) cases. Whereas H1N1 (148) cases, H3N2+H1N1, (56) cases, H1N1 + H3N2 + B were (30) cases and 130 cases were infected with type B.Current study also revealed that age group of 46 - 60 years old infected with seasonal influenza virus were (159) case including (368) male and females were (279) of different infected group..The spread of seasonal influenza virus in Najaf varies from one region to another depending on the population density. Clinical cases were collected randomly during the months of the year. It has been found that the spread of disease has something to do with temperature and humidity as high humidity and low temperature lead to increased spread of the disease because of their relationship at the stage of the spread of epidemic influenza that spread from Dec 26, 2012 and up to April 1, 2013 in addition to expatriates infected..Two techniques were used for isolation of seasonal influenza virus first inoculation of embryonated chicken egg and secondly chicken fibroblast cell culture.It was found that at the embryonated egg lethal dosage (ELD 50 / 0.5 ml) (109.6) whereas tissue culture infective dosage (TCID50 / 0.1 ml) was (109.3) of (H1N1+ H3N2) and type (B) virus..Virus was purified by two ways that are depending on the vaccine type. The gamma irradiation that dosage used for inactivating vaccine of the purified virus which was extracted from allantoic fluid and tissue culture was (5.702) mSv/h for subunit virus vaccine type whereas gamma irradiation dosage for complete (whole) virus vaccine was (8.816) mSv/h. The results proved that immunoglobulins (IgM, IgA and IgG) levels as well as (IL - 17, IL - 10 and INF - 1?) indicated significant differences among vaccinated, infected and control groups.Subunit and whole virus vaccine extracted from chicken fibroblast cell culture are considered the best of derived from virus propagated in embryonated chicken eggs.Subunit virus vaccine is better than the whole virus vaccine derived from virus propagated in embryonated chicken eggs and extracted from chicken fibroblast cell culture.The current study also reflected that locally prepared virus subunit vaccine and purified whole virus killed vaccine capable of inducing humoral and cellular immune response in experimentally vaccinated rats..Histopathological changes appeared clearly on tissue sections (lung, trachea, heart, liver, spleen, kidney) of the infected group as compared with control and the vaccinated groups.

دراسة بعض المؤشرات المناعية لدى بعض المرضى المصابين بفيروس المليساء المعدية في محافظة ديالى == A Study of Some Immunological Parameters In Some Patients With Molluscum Contagiosum In Diyala Province

Author name: رغد ياسين اعويد
Supervisor name: عباس عبود فرحان الدليمي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Diyala
First pages:
Abstract: المليساء المعدية مرض يسببه فيروس (MCV) الذي ينتمي لعائلة POXVIRUS.المليساء المعدية حيث انه مرض غير قاتل وشائع في جميع انحاء العالم, قد يشترك مع اورام المليساء التهابات قليله, المليساء المعدية يستمر لشهور او سنوات..اجريت الدراسة الحالية للفترة من 1 تشرين ا | The molluscum contagiosum (MC) virus (MCV) is a dermatotropic poxvirus, and the causative agent of MC. MC is nonlethal, common andworldwide. Additionally, little inflammation is associated with MCpapules, and MC can persist for months to years. The present study was conducted for the period from1 November 2013 to 30April, of 2014 in outpatient clinic of Baquba Teaching Hospital in city. The study aimsat assessing the immune status of patients infected with molluscumcontagiosum through the measurement ofthelevel of immunoglobulin(IgG, IgM)and the level of complementcomponents especially (C3, C4) by radial immune diffusion assay and measurement of the level of interleukin 18 R1 by Elisa assay which immune responses are key for the eventual resolution of MC. 75 patients were diagnosed with clinical lesions of MCV on different areas of the body, age of patients ranged from(2 - 50 years) including 40(53.3%) males and 35(46.7%) females.The control were 26.6±15.4 years (range from 2 - 50 years), 8 (53.3%) of them were males and 7(46.7%) females.After theexamination by single radial immune diffusion and Elisa kit.The results showed the patientswere equal or less than 16 years, 24 (32%) from 17 - 30 years, 19 (25.3%) from 31 - 45 years and 12 (16%) above 45 years and 40(53.3%) males and 35(46.7%) females, no static significant difference showed between the MCV infection and either the sex or age.The results revealed the level of IL 18R1in patient the mean±SD were 677.15±874.22 ng/ml while in control were 178.46±31.79 ng/ml. There was significant statistical difference between both groups as patients with MC had high level of IL18R1 than control.Themean±SD of IgMinpatients were 1946.6±825.6 mg/dlwhile in control were 140.1±68.7mg/dl This result was highly significant which indicates that patients with MC had higher level of IgM than control In contrast, patients had lower levels of IgG than control, The mean±SD of IgG in patientwere 221.9±96.7mg/dl while in control were 1229.9±299.7mg/dl. This result was highly significant. Also the patients had lower level of C3 and C4 than control. The mean±SD of C3 in patients were 109.6±64.8mg/dl while in control were 120.8±22.1mg/dl. The mean±SD of C4 in patients were 27.8±12.7mg/dl while in control were 38.7±9.8mg/dl. These results were statistically significant. Eleven 9 (14.7%) of patients had positive CRP while 12 (80%) of control had positive CRP which was statistically highly significant. Forty seven (62.7%) patients were from rural area while 28 (37.7%) from urban. There is no statistical difference between both groups as. Family history of MC and Recurrence of MC in patients were nostatically significant difference to infection by MCV.

التفكك الحياتي لمبيدي الملاثيون والدورسبان باستخدام مزارع احاديه وخليطه من البكتيريا == Biodegradation of Malathion And Dursban By Mono And Mixed Bacterial Cultures

Author name: فائزة كاظم عمران
Supervisor name: عادل مشعان ربيع
General topic: Biology
Specific topic: Environment
Degree: Doctorate
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: عزلت 45 عزلة بكتيرية محليه من مجموع 30 نموذج تربه ملوثة بالمبيدات والتي جمعت خلال فترات زمنيه مختلفة ومن مناطق مختلفه في بغداد. اختبرت قابلية العزلات البكتيريه على تفكيك مبيدي الملاثيون والدورسبان. اجريت غربلة اولية وثانوية باستخدام وسط الاملاح المعدنية ا | Forty five local bacterial isolates were isolated from thirty different soil sample contaminated with pesticide, These samples were collected from different Baghdad regions at different periods. The bacterial isolates were tested for their ability to biodegrade certain pesticides (Malathion and Dursban). Primary and secondary screenings were carried out using solid Mineral Salts Medium (MSM) and Liquid (MSM) with 100 ppm Malathion and Dursban at 37 0C, pH 7. Twenty seven isolates resistant to Malathion and Dursban were isolated. Out of 27 bacterial isolates from different locations in Baghdad. Results from primary screening showed that the 10 isolates had good growth, 5 isolates gave moderate growth, 12 showed weak growth and only 9 isolates failed to show any growth.According to current results, 10 bacterial isolates were tested for their ability to biodegradation of Malathion and Dursban in liquid medium, However, secondary screenings results by using minimal inhibition concentration(MIC) revealed that only four bacterial isolates had the highest ability to degrade Malathion and Dursban and subsequently were identified as Pseudomonas putida, Esherichia coli, Escherichia hermanii and Staphylococcus vitulinus by Vitek compact system respectively.The optimum conditions (incubation period, pH, temperature) for growth and biodegradation of Malathion and Dursban were examined. The obtained results indicated that the best incubation period was after 7 days, pH =6 and favorite temperature was 350C.Measurement physicochemical properties for soil pH, temperature (C°), electrical conductivity EC (µs/cm) for both Dursban and Malathion concentrations(ppm) collected from six different locations within Baghdad city. Biodegradation of Malathion and Dursban in soil inoculated with different bacterial mono - cultures and mixed - cultures were investigated. soil pH, temperature, electrical conductivity, cations and anions, on the population of native Malathion - Dursban tolerant bacteria in the cultivated soils of six sites. The result showed that soil physical properties like temperature and electrical conductivity affect significantly to native Malathion - Dursban tolerant bacterial density. Similarly, the soil chemical properties like pH, cations and anions had more effect on the abundance of Malathion - Dursban tolerant bacteria in the soil. However, the impact of soil organic carbon, organic nitrogen and available phosphorus was very significant. The results of the present study can be utilized for the development of effective bioremediation process for pesticide - contaminated soil. Under optimal conditions, Malathion and Dursban degradation was measured by using GC - MS analysis. The results revealed that Pseudomonas putida was the best isolate for degrading Dursban by monoculture isolate (47.18%) , while mixed culture(P.putida and S.vitulinus) was the best in degrading Malathion and Dursban that gave 90.84% and 81.055% respectively. Results also indicated that the number of byproducts produced by mono and mixed culture of Malathion and Dursban biodegradation. Whereas the results obtained from GC/Ms analysis revealed that not detected of toxic byproduct Malathion and Dursban degradation by Pseudomonas putida and Staphylococcus vitulins respectively.Moreover, under natural conditions.The study tested the ability of selected bacterial isolates (P. putida and S. vitulins) in biodegradation of Malathion and Dursban, the results showed slower biodegradation by selected bacterial isolates compared with laboratory conditions but study showed a intermediet product like phenol, diethyl phosphorothioate and oxon.The GC - MS results showed that the number of metabolic intermediates formed during the degradation of Malathion by bacterial mixed - culture were relatively higher in number than by bacterial mono - culture.The mass spectra of malathion containing samples treated with bacterial mono and mixed culture showed no any known toxic intermediates. The result indicated that the two main degradation products resulted from bacterial degradation, namely malathion monocarboxylic (MMA) and malathion dicarboxylic acid (MDA), the first one may convert into the latter over time. Some other degradation products may occur such as ethyl hydrogen fumarate (EHF) but in negligible amount.The result indicated that the main degradation products resulted from bacterial degradation of dursban is trichloropyridinol(TCP) and then de - chlorinated into 2 - pyridinol.The mass spectra results obtained in this study showed that the dursban was degraded to many metabolites.The results suggested that the bacterial isolates were not forming any toxic intermediates but no any intermediate was identified till R.T. 22 minutes. This indicated that dursban is probably completely metabolized by the isolates into smaller intermediatesThe isolate Pseudomonas putida isolated from soil contaminated with pesticides was identified genotypic tests. Molecular typing was performed by RAPD - PCR and comparison of the results to other Pseudomonas isolates. The result shows that genotyping differences between isolates and found the convergent percent biodegradation between this isolates of both selected pesticide, although from these differences, there was no more effect to biodegradation, which means that the biodegradation of Malathion and Dursban not related to genotype

مقارنة لتشخيص الخمج بالمقوسات الكوندية بين النساء المجهضات والولودات طبيعيا مع دراسة للتغيرات النسجية المتسببة عن الخمج == Comparative Diagnosis of Toxoplasma Gondii Infection Among Aborted & Normally Delivered Women With Study of Histological Changes As A Result of Infection

Author name: فراس محمد بشير عبد الكريم الخشاب
Supervisor name: ابراهيم شعبان داوود | صباح سعيد محمود
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة التي امتدت من بداية شهر ايار (2007) لغاية نهاية شهر اب (2008) للتحري عن نسبة الخمج بالطفيلي المسبب لداء المقوسات بين النساء المجهضات والولودات طبيعا في بعض مناطق مدينة الموصل والمراجعات لمستشفى الخنساء التعليمي للولادة والاطفال.شملت عي | This study was conducted during the period from May 2007 to August 2008 to investigate the rate of infection with toxoplasmosis among aborted and normally delivered women in Mosul City territories who attending AL - Khansa'a educating hospital.Out of 150 women under study, 50 were aborted while 100 were normally delivered. The employed tests were included latex agglutination test LATEX and Enzyme Linked Immunosorbent Assay (ELISA) with its two types (ELISA - IgM), (ELISA - IgG) which used as a high specific and sensitive test to differentiate between types of infection acute, chronic and congenital. Toxoplasmosis was diagnosed in 28(56%) out of 50 abortive placenta and in 57(57%) out of 100 non abortive placenta (normally delivered women), these samples of placenta were microscopically examined after homogenizing and staining by Gimsa and Leishman's stain The parasite development stages were also noticed including developing from the tachyzoites into the oocysts stage and the histopathological effects resulting from infection in the placenta in both aborted and normally delivered women which were represented by necrosis, vaculation and cells degeneration , inflammatory cells infiltration and the focal inflammatory reaction in the villi in addition to hemorrhage and accumulation of eosinophilic substances, the results also showed that 41 (82%) of aborted placenta and 18 (18%) of non abortive placenta (normal delivered women) were infected with Toxoplasmosis by using the laboratory Balb/c mice inoculation method while, some cases of inflammation were diagnosed in the brain, of the experimental infected mice, which were represented by the inflammatory cells infiltration and the prevailing of gliosis, tissue necrosis vaculation and cells degeneration.This study revealed that laboratory mice inoculation in the peritoneal cavity is a useful and efficient method in the detection of the parasite in infected placenta as compared with the microscopic examination of direct smears and examination of tissue sections from placenta.The present study showed an overall percentage of infection among aborted women was (92%) while (58%) showed an acute infection and (34%) chronic infection, in another hand the infection rate among normally delivered women was (70%), and it has been found the infection rate among acute and chronic cases were (41%), (20%) respectively, Its to be noted this is the first time in Mosul City for such a study conducted in this area concerning the congenital Toxoplasmosis in newborns with an infection rate (9%).In this study, sera were tested by Latex (LAT), and Enzyme Linked Immunosorbent Assay (ELISA). It was shown that IgG - ELISA is the most sensitive to diagnose the infection as compared to other tests.These study also concerned with the isolation and diagnosis of Toxoplasma gondii parasite from blood by inoculating the blood samples in laboratory Balb/C mice the rate of infection was (16%) in laboratory mice inoculated with blood collected from aborted women while the infection rate in laboratory mice inoculated by blood samples (mother and her neonatal cord blood) collected from normally delivered women appear in (12%) & (16%) respectively. The rate of occurrence of the specific antibodies against Toxoplasma gondii was identified, The highest rate was found for the type (IgG+ - IgM+), in both aborted and normally delivered women with percentage reach (40%) & (26%). The titer (100 IU/ml) of the specific IgM & IgG antibodies was found in high rate reached (75%), (43.5%) in mother blood, also found in a high percentage in the neonatal cord blood estimated as (50%), (64.4%) for the specific IgM & IgG antibodies respectively, in another hand the titer (100 IU/ml) was also found to be the more predominate titer in blood samples of aborted women with percentage reached to (50%) & (60.5%) for the specific IgM & IgG antibodies respectively.

دراسة امكانية الاستفادة من تركيز بروتين المصل الفعال نوع سي بروتين التفاعلي CRP في التشخيص المبكر للاصابات البكتيرية لحديثي الولادة وتحديد توقف اعطاء العلاج من خلاله == The Study of Usefulness of C - Reactive Protein Concentration In The Early Diagnosis And Determining The Duration of Antibiotic Therapy of Suspected Neonatal Bacterial Infection

Author name: قاسم ابراهيم حسين
Supervisor name: ايمان ناطق ناجي البياتي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: ان التشخيص السريع لاصابات تجرثم الدم في الاطفال حديثي الولادة من الامور المهمة وذلك لخطورة الاصابة المؤدية الى الموت.الغرض من دراستنا هو امكانية استخدام فحص سي - البروتين الفعال في التشخيص المبكرلهذا المرض البكتيري وكذلك استخدامه في تحديد نقطة التوقف عن ا | Rapid diagnosis of bacterial infections of newborn infants is very important itcause of morbidity and mortality. Aim of the studyThe aim of the studyis to examin the possibility of using C - reactive protein in the early diagnosis of neonatal sepsis, as well as its use in determining thestop point of the antibiotic treatment.Patent, Materials and methods This study was conducted at the Hospital of Ibn - Albaladi for children and women in Rusafathe Healthoffice / Baghdad.Iraq from February 2013 to February 2014.The number of patients with bacterial infection was 102 (62%) of the total number of patients (165) who were admitted to the neonatal care unit aged less than 28 days and their weights was more than 1500 g,. The number of males was 92 (56%) and the number of females was 73 (44%), the number of patient withearly onset sepsis EOS (less than three days) was 43 (42%) and with late onset sepsis LOS for (more than three days) was 59 (58%), 43 (57%) ofEOSwas normal deliveries infected and cesarean deliveries infected 33 (43%), while51 (57%) of LOS was normal deliveries infected and cesarean deliveries in LOS was 38 (43%). Blood samples were collected and divided into three portion : bloodculture, CRP andhematological profile.1 - Blood culture.Blood was collection from neonatal sepsis patientinto special blood culture bottle and incubate in bact /Alert 3 D device when growth of bacteria then subcultured on blood agar, MaCconkey agar, and incubatedusingvitek /2 to diagnose bacterial infection anddeterminetheir susceptibility to antibiotic treatment.ResultThe Compact results indicated that 63 (38%)of patient were negative and 102 (62%) were positive out of them 23 patients (22.6%)were infected with gram negative bacteria including {Klebsiella pneumonia 30%, Acinetobacterlowffi 17%, Enterobacter cloaca 13%, } while 79 (77.4%) were infected with gram positive bacteria including { staph Homins and staph hemolyticus each 28% then staph epidermidis 13% and staph aureus 10%, while strep spp was 6% }. 2 - c - reactive protein.It was done by twomethod for the same samples in order to characterize the accuracy of the diagnosis. The first method was agglutination test (method 1) the cut off value for positive result ? 6 µg % 79 (48%)were negative, while 86 (52%) werepositive, while the second method was immune test (method 2) µµµusing Nycocard device werethe cut off value for positive result ? 5 µg % the results 102 (62%) were positive while 63 (38% negative.3 - hematological profile.The hematological profile of our patient included PCV, WBC, neutrophil, lymphocyte, monocyte, count were measured using Emerald automated Hematology device. The results of these tests werenot compatible with the results of the blood culture, but normal result were compatible with negative blood cultures. The results adopted of blood culture confirm the presence of bacterial infection and then compared with theresults CRP test and blood profilefor the same samples.Negative result of blood culture and CRP test were 54 (33%) while positive results of blood culture and CRP test were recorded and improved after taking treatment for (, 3, 5, 7,) days for (40, 31, 5)patients respectively.ConclusionWeconcluded C - reactive protein test was useful in the diagnosis of bacterial blood infections and determine the stop - point otreatment. Gram negative bacterial isolates were sensitive impinim, livofloxacin and Amikacin while they were resistant to Ampicillin, Ampicillin /Sollbectam and Gentamycin. on the other hand gram positive bacteria were sensitive to Amikacin, Ampicillin/sulbactam Ciprphloxacin, and Tigecyclin and were resistant to Erythromicin, and Oxacillinand Benzylpencillin.

تاثير المعالجة المغناطيسية لمياه الري والسماد العضوي في نمو وانتاج القرنابيط botrytis var. Brassica oleracea صنف Solid snow == Effect of Magnetically Treatment of Irrigation Water And Organic Compost On Growth And Cauliflower Production Botrytis Cv. Solid Snow Var. Brassica Oleracea

Author name: كوثر عبد الجبار عبد
Supervisor name: ثامر خضير مرزة
General topic: Biology
Specific topic: Plant - Physiology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: ا جريت هذه التجربة في قسم علوم الحياة / كلية العلوم / جامعة الكوفة في محافظة النجف الاشرف وتضمنت مرحلتين : الاولى زراعة البذور لغرض قياس نسبة الانبات للمدة من 1 / 8 لغاية 8 / 8 / 2011 وانتاج الشتلات للمدة من 15/8/2011 ولغاية 14 / 9 /2011 في حقل خاص لانتا | This experiment was conducted in the Department of Biology, College of Sciences, University of Kufa in Al - Najaf Al - Ashraf province. It included two periods : the first was seed sowing to measure germination percentage from 1 - 8 till 8 - 8 - 2011, and transplants production from 15 - 8 - 2011 till 14 - 9 - 2011 in a private farm for vegetable transplants production. The second was transplanting in an open field belongs to Plant Production Department/Kufa Technical Institute from 15 - 9 - 2011 till 17 - 1 - 2012 to measure plant growth parameters and curd cauliflower production cv.solid snow in harmless environment techniques. The experiment was adopted as factional experiment. The treatments were distributed according to Randomized Complete Block Design (RCBD) with four replicates. The experiment included the study of two factors : the first, was five magnetic intensities for treating waters magnetically (0; 500; 750; 1500 and 2500 Gauss) by means of dipolar magnetrons. The second was three quantities of plant organic manure(rice residues).i.e.(0, 2.5 and 5.0 kg/furrow) with a length of 6.5 m. and their interactions on growth and production the yield of cauliflower, cv.solid snow. Duncan's Multiple Range Test (DMRT) was used to compare means at probability of0.05. Correlation and regression coefficient were also used to evaluate the relationships between studied parameters and time. Vegetative growth parameters that are measured were : plant leaf number ; stem height; stem diameter; plant leaf area; leaf fresh and dry weight; stem dry weight; plant shoot fresh and dry weight. Flowering parameters were : curd fresh and dry weight and curd diameter. The results could be summarized as following : 1. Water chemical analysis showed that irrigation with magnetized treated water had an effect on physical properties, nevertheless, was more or less affected ions components. 2. The use of magnetized treated water in irrigation resulted in an increase in germination percentage of cauliflower seeds cv. Solid snow.The highest germination percentage was obtained from magnetic intensity of 1500 Gauss in both experiments, laboratory and field that reached 71.14 and 65, 62%, respectively on the base of Arc Sin. 3. The use of magnetized treated water in plant irrigation at all the used intensities resulted in an early emerged curd cauliflower by 15 days compared with control treatment plants. 4. 750 gauss magnetized treated water achieved at age of 138 days from transplanting, the highest plant leaf area mean is (4836.2 cm2) with percentage increase of 51.7% compared to control treatment plant leaf area (3188.3 cm2).That effect was reflected on curd weight. 5. 1500 and 2500 Gauss magnetized treated water treatments at earlier ages increased the values of vegetative growth parameters which reflected its effect on flowering growth parameters by shorter time of curd irritation, compared with the effect of 500 - 750 Gauss at earlier ages , which are in spite of its late effects produced at 150 days old from transplanting particularly 750 Gauss the highest mean of curd fresh weight. 6. 750 Gauss magnetized treated water achieved the highest yield reached 22.2 ton/h. which differed from that productivity gained from control treatment plant(9 - 29 ton/h.) with percentage increase of 138.8%. 7. Organic plant manure addition at both quantities(2.5 and 5.0kg/furrow) resulted in an increase in both vegetative and flowering growth parameters compared with untreated plants. 5 kg/furrow treatment produced the highest means compared to control treatment particularly at the age of 138 days in all vegetative growth parameters. 8. The addition of 5 kg/furrow at age of 150 days produced the largest curd fresh weight 918.4 g. and diameter of 17.2 cm which statistically differed to compared plant treatment that produced 506.0 g. and 13.3 cm. for curd fresh weight and diameter at the same age respectively. 9. 5 kg/furrow organic manure addition resulted in a highest productivity of 21.6 ton/h. compared to that of control treatment of 11.9 ton/h. with an increase of 81.5%. 10. Interaction between irrigation water magnetically treated with 750 Gauss and addition of (organic manure with 5 kg/furrow) at the age of150 days resulted in the highest curd fresh weight of 1292.9 g. with curd diameter of 20.3 cm. compared to that plant of control treatment produced curd fresh weight of 292.5 g. and 11.0 cm. diameter. This interaction also produced 30.3 ton/h. for (750 Gauss×5 kg/furrow) compared with the productivity of control treatment plants that gave 6.86 ton/h. with percentage increase of 341.6%

دراسة كفاءة بعض المضادات الحيوية ذات المناشئ المختلفة على بعض انواع البكتريا المعزولة من حالات مرضية مختلفة == The Efficacy Study of Some Antibiotics From Different Origins On Some Pathogenic Bacteria Isolated From Different Cases

Author name: ليلى عاصي خزعل
Supervisor name: نجدت بهجت مهدي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Kirkuk
First pages:
Abstract: شملت الدراسة جمع 278عينة سريرية تضمنت نماذج من اخماج المجاري البولية ومسحات الجروح والحروق وعينات الدم ومسحات الاذن الوسطى من مستشفى كركوك العام ومستشفى طوز العام وللمدة من ( 2013 - 4 - 15) ولغاية ( (2013 - 10 - 15. اظهرت نتائج الزرع البكتريولوجي الاولي | The study included the collection (278) clinical samples included the same of urinary tract infections and swabs of wounds and burns, and blood samples and swabs from ear from Kirkuk General Hospital and Tuz General hospital in period from (2013 - 4 - 15) until (2013 - 10 - 15). The results of the first Bacteriological implant using the blood Agar and Macconkey agar emergence of planting positive in (148) of the total sample (278) sample rate (53.33%). Diagnosed colonies developing depending on planting characters and biomicroscopy and tests biochemical as possible diagnosis (90) the isolation of clinical divided to (30) isolation of each of the bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) divided to (28) isolates of Urine and 21 isolated from wounds and 25 isolates of burns and 11 isolated from blood and 5 isolates from the ear of the total isolates, confirmed diagnosis using the API Staph System and API 20E system. The study included examination of sensitivity using discs toward the 15 antibiotic for three types. The isolates showed to different in resistant rate, and was Anti Chloramphenicol is the most effect on the bacteria S.aureus where all isolates were sensitive to him 100%, and was an anti Imipinem, Nitrofuranation the most effect on bacteria E.coli where the percentage of sensitivity to two anti - (90%), either Anti Amikacin was the most impact on the isolates of Ps. aeruginosa where all isolates were sensitive to him ratio (100%) I tested the sensitivity of bacterial species toward (5) antibiotic (Amoxicillin Augmantine, Cefotaxime, Ciprofloxacin, Tetracyclin) concentrations of certain several different origins for each antibiotic using the method of casting dishes, use an anti Amoxicillin concentration (25) mg / ml and origins different (Iraqi, Indian, Turkey, United Arab Emirates) The results show that the origin United Arab Emirates is the most efficient than the rest of other. Use the adversaries (Cefotaxime, Augmantine) concentration (10) mg / ml and three - origins (Turkish, Indian, United Arab Emirates), oukd show that Origin United Arab Emirates is the most efficient of the rest of the origins Other. also used anti Tetracyclin concentration (10) mg / ml for three origin (Iraqi, Indian, Chinese). show that Origin Iraqi is the most efficient of the rest of the origins Other.and used anti Ciprofloxacin concentration (5) mg / ml for four origin (Iraqi, Indian, Turkish, United Arab Emirates) and show that the United Arab Emirates and Turkish origin is more efficient than the rest of other origins.Bacterial isolates showed multiple drug resistance to antibiotics where all isolates Ps. aeruginosa with resistance to multiple antibiotics by (100%) and showed isolates of S.aureus resistant multi - rate (93.3 %) and showed isolates of E.coli (96.6%) as well characterized bacterial isolates in multiple resistance to antibiotics Alpittalaktam particularly where the rates of multiple resistance (93.3 %) of the isolates of E.coli and (100%) isolates Ps.aeruginosa and (93.33%) of the isolates by S.aureus.the rapid Iodine standard method was used for screening for susceptibility of bacterial isolates to produce enzymes Beta - Lactamase have shown (73) the isolation of a positive result from the total (90) isolation rate (81.1%), were distributed among the isolates produced (27) isolated from bacteria by S.aureus (90 %) and (25) isolates of E.coli bacteria by (83.3%) and (21) isolated from bacteria Ps. aeruginosa (70%).to detect broad - spectrum Beta - Lactamase enzymes is used discs method adjacent and the results showed that there are (24) isolation of the total (73) is positive for examination by (32.87%) distributed on (12) isolated from bacteria S.aureus by (44.44%) and (5) isolates of E.coli bacteria (20%) and (7) of the bacterial isolates Ps. aeruginosa by (33.33%).Tested susceptibility bacterial isolates to produce mineral Beta - Lactamase enzymes Metallo ? - Lactamase using IMP - EDTA Combination disc and three isolates can from the production of the enzyme (4.10%) by two isolates of bacteria Ps.aeruginosa by (9.52%) and one isolate of the bacterium E.coli (4%).

تحديد شدة الاصابة الحادة والمزمنة لداء المقوسات الكوندية بواسطة مستويات (IL - 6, IL - 8, TNF - ?) == Determination of Acute And Chronic Toxoplasmosis By Estimation of IL - 6, IL - 8 And TNF - ? Levels

Author name: لينا قاسم كاظم الزهيري
Supervisor name: بان نوري القاضي
General topic: Biology
Specific topic: Zoology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: المقوسات الكونديه من الطفيليات الشديده النجاح في انشاء اصابة مزمنه طويلة الامد. وقد اجريت الدراسة الحاليه على 366 امراة (117 مجهضات، 141 حوامل، 108 غير متزوجات) جمعت من مستشفيات مختلفة في بغداد. جمعت عينات الدم لجميع المجاميع المدروسة خلال الفترة مابين تش | Toxoplasma is a highly invasive parasite which establishes a life - long chronic infection. The present study was performed on 366 women (117 miscarriages, 141 pregnant and 108 singles) aged (17 - 35) years old from different hospital in Baghdad. Blood samples were collected from all patients during the period of Oct.2013 until of Jan. 2014. Anti - toxoplasma antibodies in the sera of all samples by using two tests, Latex test (LAT) and ELISA (IgG), (IgM) while, the proinflammatory cytokine were estimated by evaluation of the cytokine such as (IL - 6, IL - 8, TNF - ?). The results were showed that : ? The infection rate of toxoplasmosis by LAT was (41.53%) which was more sensitive than ELISA IgG (36.85%), and miscarried women were diagnosed by higher anti - toxoplasma IgG antibodies 62/117(52.99%), while pregnant result were lower 30/108 (27.78%). ? The most frequent age group of chronic infection with T.gondii was (23 - 28) year in both miscarriage and pregnant women, and represented by 45.16%, 40% respectively. While the most frequent age group for a single woman was (17 - 22) years, and represent 46.511% of the total number of this group.? Seroprevalence of toxoplasmosis by ELISA IgM test revealed low sensitivity in different studied groups 25/366(6.830%), and miscarried women were diagnosed by a highly significant (P ?0.01) percentage of acute toxoplasmosis14/117(11.965%).and The age group of (17 - 22) years old diagnosed high significant (p?0.01) percentage of acute toxoplasmosis in all studied groups.? According to the distribution of infected miscarried women with toxoplasmosis and gestational age by ELISA IgM the result showed that most of miscarriages were occurred at the first trimester which considered high significant (p? 0.01) 8/14(57.42%). While, chronic infected women at the second trimester (4 - 7 month) diagnosed by significantly (P?0.01) higher percentage of miscarried 24/62(38.71%).? The level of pro - infalmmatory cytokine (IL - 6) in acute infected miscarried women with toxoplasmosis distincted with a significantly high increase (P? 0.05) (177.31±12.53 Pg/ml) in comparison to chronic infected one (119.36 ±14.07 Pg/ml).While, chronic infected pregnant women was low significantly (P ?0.01) (48.41± 2.79 Pg/ml) in comparison to acute infected one and other studied groups, ? The acute infected singles was (118.98± 15.68 Pg/ml) higher non - significantly (P?0.05) than chronic infected one (112.65 ± 17.91 Pg/ml) and both disease activity were significantly higher than its level in healthy singles.? The level of TNF - ? in acute infected miscarried women distincted with significantly higher (P?0.05) (97.46 ± 5.37 Pg/ml) in comparison to chronic infected women (69.55 ± 4.58 Pg/ml), Whereas the mean level of TNF - ? in pregnant infected women during acute phase (62.31 ± 3.82 Pg/ml) was significantly (P?0.05) higher than chronic infection (21.42 ±2.74 Pg/ml)? Single infected women showed high significant increase (P?0.05) of TNF - ? level (50.48 ± 7.42 Pg/ml) in comparison to chronic infected one (35.46± 5.36 Pg/ml) and both disease activity scored high significant increase (P?0.05) of TNF - ? in comparison to healthy singles (18.29 ± 1.02 Pg/ml).? Finally, the mean level of IL - 8 in miscarried women with positive infection with T. gondii during acute phase (187.92 ± 15.38 Pg/ml) was significantly (P?0.05) higher than chronic phase of infection (140.57 ± 13.87 Pg/ml).Whereas the mean level of IL - 8 in acute infected pregnant women (110.32 ± 9.13 Pg/ml) was higher significantly (P?0.05) than healthy pregnant (91.49 ± 7.59 Pg/ml) and healthy singles (88.82 ± 5.62 Pg/ml).? Infected single women showed highly significant (P?0.01) increased levels in both diseases activity in comparison to single women without infection (88.82± 5.62 Pg/ml).

دراسة بكتريولوجية لبعض الاجناس البكتيرية السالبة لصبغة غرام المقاومة لمضادات البيتالاكتام والمعزولة من اخماج المجاري البولية في محافظة ديالى == Bacteriological Study of Some Genus For Bacterial Gram - Negative Resistant To ? - Lactam, Isolated From Urinary Tract Infections In Diyala Province

Author name: محمد خضير عباس النعيمي
Supervisor name: عباس عبود فرحان الدليمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Diyala
First pages:
Abstract: شملت الدراسة 300عينة جمعت من مرضى مصابين باخماج المجاري البولية وتم جمع العينات في مدينة بعقوبة من مستشفى البتول للولادة والاطفال ومستشفى بعقوبة التعليمي، للمدة بين 1/9/2013ولغاية1/1/2014.اظهرت نتائج الزرع البكتيري على اوساط اكار الماكونكي واكار الدم ووس | Three hundred urine from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al - Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results reved that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli, 22(33.33%) Proteus mirabilis, 9(6.06%)Klebsiella pneumonia, 5(7.57%) Enterobacter spp for 4 (25%) Enterobacter cloacae and 1(5.51%) Enterobacter aerogenes and5(7.57%) Pseudomonas aeruginosa by using diagnostic phenotypic, biochemical tests and confirm the diagnosis using regular aPI20E. Investigation of some virulence factors showed that both Escherichia coli and Proteus mirabilis produced haemolysin 52% and 90.9% respectively. The production of biofilm by local isolates was detected in Congo - red way, Isolates of Escherichia coli and Proteus mirabilis had been shown the ability to produce biofilm 92% and 90.9% respectively. The results showed that all isolates of Proteus mirabilis produced urease by 100%, while the results detect that the isolates of Escherichia coli not able to produce this enzyme. Proteus mirabilis showed ability to produce swarming with 100%. Siderophore production by Escherichia coli and Proteus mirabilis was 48%, 9% respectively. Eschrichia coli, and Proteus mirabilis produced bacteriocin with percentag of 32% and 50% respectively.The production of ? - lactamase by Eschrichia coli and Proteus mirabilis was 60% , 40.9% respectively, also the isolated had the ability to produce the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, The production from each ofEschrichia coli and Proteus mirabilis 12%, 31.8 % respectively. Results of Metallo? - Lactamase by using the Imp - EDTA combination indicated that E.coli and P.mirabilis were 12% and 13.6% respectively. Isolates of E.coli showed highest resistance rate 92% for Augmentin while isolates of P.mirabilis showe higher resistant to cefotaxime 81.8%. The results showed that multiple resistance pattern for antibiotic 43 (91.5%) resistant to (2 - 5) antibiotics, Isolates of E.coli showed highest multiple resistance rate 92% while isolates of P.mirabilis rate 90.9%. The Minimal inhibitory concentration (MIC) for namely two antibiotics Cefotaxime, Ceftazidime were determined.MIC values of these antibiotics ranged between (2 - <1024), (4 - <1024) µg\ml E.coli and (2 - <1024), (8 - <1024) µg\ml P.mirabilis respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique, (9) samples from (10) total, divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.

دراسة وراثية وبكتريولوجية على بكتريا تثبيت النيتروجين التكافلية Ensifer meliloti == Bacteriological And Genetic Study On Symbiotic Nitrogen Fixing Bacteria Ensifer Meliloti

Author name: محمد زهير ناجي المرعب
Supervisor name: حسن فاضل ناجي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: Arabic
University location: Babylon
First pages:
Abstract: عزلت عشر عزلات من بكتريا Ensifer meliloti من العقد الجذرية لنبات الجت Medicago sativa L. بعد ان جمعت من مناطق مختلفة من محافظة بابل خلال المدة من شهر تشرين الثاني/2012 لغاية شهر شباط/2013. شخصت العزلات جزيئيا بتقنية تفاعل البلمرة السلسلي فضلا عن طرق ال | Ten isolates of Ensifer meliloti were isolated from root nodules of Medicago sativa L. collected from different areas of Babylon province during a period from November - 2012 to February - 2013. The isolates were molecular identified using polymerase chain reaction technique, in addition to traditional methods (morphological and biochemical). All isolates were harboured nodbox and mucR genes and produced cyclic ? - (1, 2) glucans, cellulose fibers and lipopolysaccharide.The ability of isolates to grow at different degrees of pH, temperatures and different concentrations of sodium chloride were tested. It was found that the optimum pH for growing of Em1, Em3, Em4, Em6 and Em8 isolates was pH7, while for the isolates Em2, Em5, Em7, Em9 and Em10 was pH8. The optimum temperature for growing of Em1, Em3, Em5, Em7, Em8 and Em9 isolates was 30?C, while the optimal growth of Em2, Em 4 and Em6 isolates was 35?C, Em10 isolate showed significantly extremism to grow at temperture reached to 45?C. The optimal percentages of NaCl for growing of Em1, Em3, Em4, Em5, Em6, Em8 and Em9 isolates were 2%, for Em7 isolate was 3% and for Em2 and Em10 isolates were 4%. The effect of different degrees of pH on symbiotic properties was studied. Em1 and Em8 isolates that grew at pH6.5 ; all isolates that grew at pH 7, pH 7.5 and pH 8 were able to infect the root hairs of alfalfa plants. The isolates that grew at pH6.5 (except Em1 and Em8 isolates) and all isolates that grew at pH8.5 were failed to infect the root hairs and did not formed nodules and resembled to the control plants (without inoculum).Different concentrations of NaCl on symbiosis was studied, it was found that all isolates that grew at 2% and Em2 and Em10 isolates that grew at 3% formed nodule, while Em1, Em3, Em4, Em5, Em6, Em7, Em8 and Em9 isolates that grew at 3% and all isolates that grew at 4%, 5% and 6% did not elicited nodules on the roots of alfalfa plant. Light microscopic observations of root hairs inoculated with Ensifer meliloti isolates showed the root hair deformations like curling, waviness, swelling and formation of shepherd`s crook and infection thread. These consequent changes lead to emerge the mature nodules having five distinct zones viz., distal meristematic, infection, inter, nitrogen - fixing and proximal senescence zones.The gel electrophoresis results of plasmid DNA of isolates showed that these isolates harboured two megaplasmids. When the isolates exposed to extreme factors like pH, temperture and NaCl, it was found that the isolates lost these plasmids and its ability to elicite the nodules and failed to fix the nitrogen. This study showed that the nitrogen fixing bacteria had essential role to support the plant growth and increase their productivity, and recommended to use the isolate Em10 as example for biofertilizers and the best alternative to the chemical fertilizers

دراسة التاثيرات السمية للـ Gliotoxin المنتج من العفن Aspergillus fumigatus باستخدام تخمرات الحالة الصلبة == Study Toxicity Effects of Gliotoxin Produced By Aspergillus Fumigatus Using Solid State Fermentation

Author name: محمد عادل نوري
Supervisor name: عبد الكريم جاسم هاشم
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: تم اختبار قابلية عشرة عزلات للعفن Aspergillus fumigatus على انتاج الغليوتوكسين بوساطة تخمرات الحالة الصلبة وباستخدام الرز كوسط زرعي (ركيزة). اشارت نتائج الغربلة ان العزلة AF - 5 كانت هي الافضل واعطت اعلى انتاجية.كانت الظروف المثلى لانتاج السم على وسط الر | The ability of Ten Aspergillus fumigatus isolates for gliotoxin production were screened by solid state fermentation (SSF) using rice medium as substrate. The results indicated that, the AF - 5 isolate was the highest gliotoxin producer.The optimum conditions for gliotoxin production by AF - 5 isolate on rice medium were : - inoculum size 6×106 spores, moisturizing ratio (5 : 1) (w : v) with distilled water and incubation at 37 ?C for 10 days. Gliotoxin was purified from crude extract of AF - 5 isolate after production under the optimum conditions using solid state fermentation SSF. Purification of gliotoxin was achieved by two steps including : filtration and solid phase extraction. These processes were accessed to remove debris from crude extract and gives pure gliotoxin. The gliotoxin concentration was (122.6 ppm), which was detected by Thin layer Chromatography and High Performance Liquid Chromatography.The result of cytotoxicity effect of both purified extract and standard gliotoxin on human lymphocytes with exposure time of 24 hours at four different concentrations 25, 50, 100 and 200 ppb showed growth inhibition percentage 21, 39.10, 61.99, 87.45% and 17.89, 34.92, 58.34, and 85.22% respectively. The cytotoxicity effects have been done using conversion of the MTT to MTT formazan. After DNA was extracted from lymphocyte treated with toxin and analyzed by electrophoresis on a 1% agarose gel, gliotoxin appeared to have ability to damage the DNA. Results showed that both growth inhibition and DNA damage were increased gradually with the increasing of gliotoxin concentration.

دراسة انتشار انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا == Studying The Spreading of Fungus Saprolegnia SPP. In Waters of Diwaniyah City And Characterization The Molecularly

Author name: معتز محمد عزوز الزاملي
Supervisor name: ماجد كاظم عبود الشبلي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: شملت الدراسة الحالية عزل وتشخيص بعض الفطريات المائية ومنها انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا. عزلت الانواع التابعة لهذا الفطر وهما Saprolegnia parasitica وSaprolegnia ferax من الانهار والبحيرات والاسماك, وتم قياس الخصائ | The present study is concerned with isolation and identification of some aquatic fungi, including species of Saprolegnia spp. in waters of Al - Diwaniyah city and molecular characterization. Two species, Saprolegnia parasitica and Saprolegnia ferax, have been isolated from rivers, lakes and fishes. The physical and chemical environmental characteristics have been measured. The types above have been examined to extract the DNA. The (PCR) technique has been used to examine the presence of protease enzyme. The results of isolation and identification showed that the percentage of the two species were 64% in rivers areas 40% in the lakes areas and 50% in fishes. The rates represented the examined samples which were 60 samples. AS well as isolates was in rivers 16 isolation and in Lakes 10 isolates either in fish was 5 isolation and thus the total isolates 31 isolation, The S. parasitica species is more apparently found during (February, 2014), the number of samples was 12, the less presence of this species in (April, 2014), where the number of sample, was 3. The S. ferax species is more apparently presented in (February, 2014), the number of samples was 10. The less presence was in (April, 2014), the number of samples was 2. The results showed that the number of isolation of Saprolegnia spp. study area as it was S.parasitica species in were 6 in rivers, 2 in lakes, and 3 in fishes. The second species, S. ferax showed number of isolations which were 10 in rivers, 8 in lakes, and 2 in fishes. The PCR examination has used to detect the rDNA gene which responsible for proteases gene and whether it is found in the two species of fungi. The express way has been used to extract DNA and according to the instructions of manufacturer company. The concentrations of DNA in first species were 121.8 - 73.0 mcg/microliter and purity 1.87 - 1.80.In the second species the concentrations of DNA were 2360.4 - 166.9 mcg/microliter and purity 2 - 1.75. The results, by using PCR technique, showed that the percentage of protease in first species was 25% in rivers. In lakes and fish was 100%.Concerning the second species, the percentage of enzyme was 42% in rivers, 40%in lakes, and 50% in fishes. It can be concluded that there is a diversity in fungi species with respect to study areas and enzyme secretion in the stages of fungi infection. The enzyme is more apparently found in S.parasitica species compared with S.ferax species.

دراسة مصلية دمية لداء المقوسات في النساء الحوامل والاطفال حديثي الولادة في محافظة القادسية == Bloody Serological Study of Toxoplasmosis In Pregnant Women And Newborns In Diwaniyah Province

Author name: مروة جبار مزهر العبادي
Supervisor name: خيري عبد الله داود العكيلي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Qadisiyah
First pages:
Abstract: اجريت الدراسة الحالية على الاطفال حديثي الولادة في محافظة الديوانية للمدة من بداية شهر تشرين الثاني 2012 الى نهاية شهر نيسان 2013.وقد جمعت عينات الدراسة من دم الحبل السري للاطفال الرضع المولودين حديثا اثناء الولادة في مستشفى الاطفال والولادة التعليمي في | The present study was done on new born babies in Diwania province during a period from the beginning of November 2012 till the end of April 2013. Blood samples were collected from the umbilical cord of the new born babies during delivery in children and maternity teaching hospital. Fifty samples were collected separated to serological tests and for blood pictures. Serological tests were used in this research as follows (Rapid IgG - IgM test, latex test and ELISA IgG, IgM). The results showed the Rapid test was the easiest and cheapest test for diagnosis specific antibodies against Toxoplasmosis, therefore the positive samples were 14% for IgG, 4% for IgM and 36% for overlap cases. While the positive results for latex test was 14% but the highest result was recorded by using ELISA test 30% for IgG, No positive result recorded for IgM. There were variation in positive cases of different ages of mothers, the highest percents found in ages between 30 - 39 years old by using Rapid test 72.72% , while by using The latex test demonstrate high percent of infection was 20% and also for ages between 15 - 19 year old, and the ELISA recorded 50% and the same group of age 15 - 19 years old. The results showed the for the residence factor by using Rapid test showed the rural people were high percent of incidence of Toxoplasmosis 57.14%, but the urban people recorded Latex test and ELISA)17.24%, 31.03%) respectively. Aborted women in previous time were followed by using the serological tests Rapid test 10%, Latex 10% and ELISA zero. The incidence of Toxoplasmosis was high in housewives more than other career, the results were) 34.14%, 34.14 (% for Latex and ELISA, IgM and overlapping cases, while in other career by using Rapid test 66.66% Results showed standard Blood parameters did not register significant differences (P> 0.05) for the total number of white blood cells WBC, hemoglobin Hb, blood volume compact PCV, Erythrocytic RBC, and differential count of white blood cells for Neutrophiles, Lymphocyte, Monocytes, Basophiles and Eosinophiles. Phagocytic activity was prominent in adult people more than newly born babies.

تحديد بعض المعادن الثقيلة والتلوث البكتيري في الحليب الخام والمستورد المجفف == Determination of Some Heavy Metals And Bacterial Contamination of Raw And Imported Powder Milk

Author name: مروة صباح طالب
Supervisor name: محمد نافع علي العزاوي
General topic: Biology
Specific topic: Plant
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: يتعرض الحليب للعديد من الملوثات الفيزيائية والكيميائية والبيولوجية وذلك بسبب طرق الانتاج واسلوب النقل وعمليات التسويق ومن هذه الملوثات المعادن الثقيلة والرقم الهيدروجيني ودرجة الحرارة والبكتيريا. وقد صممت الدراسة الحالية لفحص عينات للحليب الخام التي جمعت | Milk is subjected to various physical, chemical and biological pollutants duo to producing, transporting and marketing processes such as certain heavy metals, pH, temperature and bacteria. The current study was designed to examine fresh milk samples were collected randomelly at early morning from six different locations at a rate of once each month during study period which commenced in October 2013 and ended in march 2014 and powder milk brands which were examined again for six months as in case of fresh milk where these milk brands were collected from local markets each month. The results of heavy metals showed that : • The investigation of Lead content in raw milk revealed that the highest mean value (1.801±0.311 ppm) was scored in Abo - Ghraib sample and the lowest mean value (0.941±0.104 ppm) was observed in Ghazaliya sample. For the Cadmium content in raw milk that the highest mean value (1.532±0.124 ppm) was found in Abo - Ghraib sample and the lowest mean value (0.063±0.044 ppm) was found in Fal'loga. The Copper content in raw milk also investigated and resulted the highest mean value was (0.931±0.092 ppm) in Azizia milk sample and almost similar value (0.931±0.301 ppm) was found in Madain sample while the lowest mean value was (0.308±0.029 ppm) in Fal'loiga sample. The highest mean value of Potassium in raw milk was recorded in two samples in Azizia (9718.8±246.6 ppm) and (9718.3±482.2 ppm) in Abo - Ghraib but the lowest mean value (4156.3±268.3 ppm) was recorded in Essaouira. Regarding Sodium content in raw milk, the results have found that the highest mean value was (2968.8±312.2 ppm) in Essaouira sample while the lowest mean value was (1432.5±156.8 ppm) in Abo - Ghraib. The Chloride content in raw milk samples, the highest mean value was (3053.0±128.6 ppm) recorded in Fal'loga sample and the lowest mean value was (639.0±198.4 ppm) was found in Azizia sample. Analysis of variance of these data showed significant effects (P?0.001) of both sampling sites and collecting months on milk (pb, Cd, K, Na and Cl) contents while analysis of variance of these data reveals no significant impact of sites on milk Cu content (P> 0.05) where no differences were observed between mean value of different collecting sites but shows highly significant (P? 0.001) effects of collecting months.• While powder milk has shown that the highest mean values of Lead (1.225±0.052 ppm) were found in Al - Mudhish brand and the lowest mean value (0.088±0.002 ppm) was detected in Anchor. The highest mean Cd value was (0.184±0.005 ppm) in Nido brand and the lowest mean value was (0.069±0.003 ppm) in Altunsa sample. While the highest mean value of Copper was (1.656±0.254 ppm) in Dielac powder brand and Anchor milk brand had the lowest copper mean value which was (0.767±0.014 ppm). The concentration of Potassium was (9625.0±462.9 ppm) which is the highest recorded in Dielac milk sample and the lowest mean value (3356.8±225.6 ppm) was found in Al - Marai milk sample. While the highest average of Sodium was (2625.0±342.6 ppm) scord in Dielac brand and the lowest average was (1745.0±338.4 ppm) recorded in Al - Marai sample. The highest mean value of Chloride (193.0±41.0 ppm) was recorded in Nido milk brand and the lowest mean value (122.7±22.2 ppm) was found in Altunsa milk sample. However, analysis of variance shows significant differences (P?0.001) between examined milk brands while no such differences were found between these brands (P>0.05) regarding collecting months.From the above results, it seems that the highest content of (Pb, Cd and Cu) are higher than those of standards of the International Dairy Federation (IDF) while contents of (K, Na and Cl) are within the standard requirement according to National Research Council (NRC).Microbial tests of raw milk samples were positive for bacterial growth, as different bacterial species were observed such as Lactobacilli spp. 63.8%, Streptococcus spp. 61.1%, Staphylococcus aureus 44.4%, Escheritia coli 88.8%, Bacillus spp. 72.2%, Salmonella typhi 72.2%, Staphylococcus epidermidis 30.5%, Pseudomonas spp. 47.2%, Micrococcus spp 27.7%, Clostiridia spp. 66.6%, Enterococci spp. 11.1% and K. pneumonia 75% from bacterial contamination of raw milk samples.However, only (11.1%) of the total collected samples of raw milk were suitable for human consumption and (88.9%) unsuitable, indicating that the source of infection or contamination of milk may be due to the animal health, human handler and the environmental factors, e.g., contaminated vessels, polluted water, flies and dust, etc… Obviously, bacterial contamination test of powder milk samples was carried out and negative results were obtained. However, it seems clearly that the opportunity of finding bacterial contamination in powder milk samples may be very rare since milk powder is produced in well designed and modern techniques. Nevertheless, a single bacterial contamination test has indicated to a positive growth of Staphylococcus aureus colonies.

تاثير المجال الكهرومغناطيسي في بعض صفات النمو والحاصل والانقسام الخلوي لنبات العصفر Carthamus tinctorius L == The Effect of The Electromagnetic Field On Some Growth Characteristics And Yield And Mitotic Index For Carthamus Tinctorius L

Author name: مروة قيس ابراهيم العبيدي
Supervisor name: ماهر زكي فيصل الشمري
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: اولا : - التجربة الحقلية : اجريت التجربة في الحقل التابع لقسم علوم الحياة - كلية التربية للعلوم الصرفة - ابن الهيثم، وصممت باستخدام تصميم القطاعات الكاملة المعشاة Randomized Complete Blocks Design (RCBD) حيث تم تعريض البذور قبل الزراعة لمجال كهرومغناطيسي | First : - Field experience : the experiment was conducted in the Department field of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using design Full sectors Randomized Complete Blocks Design (RCBD) where the seeds exposing to electromagnetic field in the Department of Physics - Electrical Laboratory - College of Education, Pure Science - Ibn al - Haitham before planting them in five intensities (0, 5, 10, 15, 20) mT and two (1.2 and 3) an hour for each intensity to see the electromagnetic field effect in some of the qualities of growth and yield plant Safflower the results were as follows : The effect of electromagnetic field strength was positive traits and was the highest increase in exposure to the intensity of 10 mT for each of the (percentage of germination, root size of the second date, plant height, leaf area, leaf area guide, the dry weight of the root system at the time the second, fresh weight of shoots at the first appointment, the average absolute growth, the sustainability of biomass, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (81.21%, 18.33 cm 3, 93.37 cm, 71.29 cm 2, 1.187, 8.21 g.plant 1, 155.13 g.plant - 1, 1.76 g.yum 1, 691.67 g, 2.03%, 0.733%, 2.47%, 12.96%, 56.96 spad, 44.55 Nora.plant - 1, 46.60 g.plant - 1, 5.35 g, 161.80 kg. acres - 1, 38.74 mg. plant - 1, 12.09%, 13.50%), respectively, except for the size of the root first date, fresh weight of the total root for the first two dates, and second, the dry weight of the root system in the first date, fresh weight of shoots in the second deadline, the dry weight of shoots for the first two dates, and second, the specific weight of the oil had the highest increase at exposing the seeds to the intensity of 15 mT and average (15.16 cm, 15.12 g. Plant - 1, 26.00 g plant - 1, 3.98 g plant - 1, 411.08 g.plant - 1, 20.03 g. Plant - 1, 69.15 g. Plant - 1, 1.069%, respectively) and the highest increase of the proportion of total oil when exposure to the severity of 5mT an average 33.94%. The effect of the length of time in field was significant in most of the qualities which gave the term two highest increase with the exception of germination percentage was the highest increase in exposure for a period of three hours, with an average (60.79%, 91.01 cm, 67.51 cm 2, 1.124, 131.93 g. Plant - 1, 337.61 g. plant - 1, 1.68 g. Day - 1, 1.92%, 12.04 g. plant, 55.99 Spad, 146.06 kg. dunams - 1, 4.77 g, 40.23 mg. plant - 1, 28.03%) for each of the (germination percentage, plant height, leaf area, leaf area guide, fresh weight of shoots in the first date, fresh weight of shoots in the second deadline, the average absolute growth, nitrogen, protein, chlorophyll, yield seeds, 100 - seed weight, carbohydrate content, the total percentage of oil). As was the overlap between field strength and duration significant effect on most of the traits with the superiority of treatment (10 mT for two hours) on the rest of the other transactions for each of the (germination percentage, the size of the root of the first two dates, and the second, plant height, number of branches, leaf area, area guide paper, fresh weight of the root system of the first two dates and II, , dry weight of the root of the first two dates, and the second, fresh weight of shoots for the first two dates, and second, the dry weight of shoots for the first two dates, and second, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, He yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (39.33%, 16.50 cm, 19.50 cm, 103.86%, 17.00, 79.83 cm, 1.330, 16.43 g.plant - 1, 29.20 g. plant - 1, 4.16 g.plant - 1, 10.48 g.plant 1, 184.06 g. plant - 1, 416.33 g. plant - 1, 22.35 g. plant - 1, 73.25 g. plant - 1, 2.10%, 0.800 %, 3.04%, 13.15%, 60.31 spad, 49.14 Nora.plant - 1, 53.37 g. plant - 1, 6.14 g 185.97 kg. acres - 1, - 1.plant 47.81 mg, 2.28%, 14.25%), respectively, with exception each of the average of the absolute growth and sustainability of biomass, where the highest increase in exposure to the intensity of 10 mT for a period of three hours and average (1.85 g.yum 1, 724.85 g.yum), respectively, and total percent of the oil was the highest increase in exposure to the intensity of 5 mT and for three hours and an average of 37.28% and the intensity of 15 mT for a period of three hours, specific gravity of oil and an average of 1.081%Second : - laboratory experiment : experiment was conducted in the laboratory of physiology of the plant Higher Studies - Department of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using the full random design Completely Randomized Design (CRD) to study the electromagnetic field effect five intensities (0, 5, 10, 15, 20) mT and two (1.2 3) per hour on the severity of the cell division of the safflower plant results were as follows : The results showed that there is a positive effect of the electromagnetic field on the traits and was the highest increase at exposing the seeds to the intensity of 10 mT for each of the (number of dividing cells of the tops of developing the parties to the roots, the total number of cells to the tops of the parties to the roots, and guide cell division developing the tops of the parties to the roots) and average (156.00% 1143.00%, 12.17%), respectively. The effect of duration of exposure, and had a significant effect on the average number of total cells to the tops of the parties to the roots reached 1042.20% when the exposure for two hours.The effect of overlap between the severity and duration of the electromagnetic field significant was effect in all cellular qualities and was the highest increase in exposure to the intensity of 10 mT for a period of one hour and average (167.00%, 13.52%) for each of the number of dividing cells developing the tops of the parties to the roots and guide cell division to the tops of developing the parties to the roots, The total number of cells to the tops of the parties to the roots was the highest increase in exposure to the intensity of 10 mT for two hours and an average of 1203.50%.

التحري عن جين الاوتولايسين في المكورات العنقوديه البشروية المقاومة لمضاد الفانكومايسين == Autolysin Gene Detection In Vancomycin Resistant Staphylococcus Epidermidis

Author name: مريم خميس عبد ربة بريس
Supervisor name: مي طالب فليح
General topic: Biology
Specific topic: Microbiology
Degree: Master
University: University of Baghdad
Language: English
University location: Baghdad
First pages:
Abstract: من مجموع مئة عينة سريرية جمعت من مصادر مختلفة شملت الحروق والدم والجروح ومسحات انفية, تمكنت 90 عزلة من النمو على اكار المانيتول الملحي , ومن بينهم 40(44.4 %) عزلة عائدة لبكتريا المكورات العنقودية المنتجة للانزيم المخثر للبلازما و50 (55.5%) عائدة لبكتريا ا | Out of one hundred clinical samples were taken from different sources which include burns, blood cultures, wounds and nasal swabs infections ; 90 isolates developed growth on mannitol salt agar. Among these, 40 (44.4%) were Coagulase positive (Staphylococcus aureus) isolates, 50 (55.5%) belong to coagulase negative staphylococci in which Staphylococcus epidermidis isolates were 30(60%). The pattern of antibiotic susceptibility of Staphylococcus epidermidis isolates to 12 antibotics (Amoxiclav, Ceftazidim, ciprofloxacin, clindamycin, Erythromycin, Gentamycin, Imipenime, Penicillin G, Tetracycline, Rifampin , Methicillin, and Vancomycin) were determined using disc diffusion method. The results revealed that resistance to Penicillin G10 and Amoxiclav (Amoxicillin - clavulanic acid) were 100%, Methicillin were 93%, Erythromycin were 90%, Gentamycin and Clindamycin were 70%, Tetracycline and ceftazidim were 75%, Ciproflaxacin were 60%, Rifampin were30%. 95% of S.epidermidis isolates were sensitive to Imipenim and 5% of them were intermediate resistant, while these isolates showed 90% sensitivity to vancomycin. 19 isolates were multidrug resistance. Minimum inhibitory concentration of S.epidermidis isolates to vancomycin, was determined. The results revealed that (12) S.epidermidis isolates (40 %) were vancomycin resistant, the MIC of them were between 256 ?g\ml and 32 ?g\ml, (4) S.epidermidis isolates (13.3%) were intermediate resistance, the MIC to 3 of them were 16 ?g\ml and the last was 8 ?g\ml. Some virulence factors of VRSE and VSSE were detected including the hemolysin, protease, lipase and urease. The S. epidermidis isolates were produce hemolysin, protases, lipase and urease, were 100%, 100%, 25%, 100% respectively in VRSE, while in VSSE 100%, 100%, 60%, 100% respectively. The isolates were subjected to polymerase chain reaction (PCR) technique in monoplex pattern to amplify resistant incoding gene : the vanA, vanB and autolysine gene aae gene. The results by this study showed that 12 (40%) S. epidermidis isolates gave the implicone size (1030 base pair) of the vanA gene. However the results of MIC and PCR were similar but no any isolates gave product for presence of vanB gene. All S.epidermidis were able to produce implicone size(858bp) of aae gene. The effect of vancomycin resistant S. epidermidis on cell autolysis activity was detected by whole cell autolytic assay.The results revealed that there was significant difference among three isolates, the VSSE isolate (S.epidermidis 22) have the highest autolytic activity in the presence of antibiotic, followed by the VRSE isolate (S. epidermidis 1) and the VISE isolate (S. epidermidis14) which was the lowest autolytic activity with the presence of antibiotic. The result of transmission electron microscope (TEM) showed that the VRSE isolates (S.epidermidis 1) have thicker cell wall followed by VISE (S.epidermidis 14) isolates.However, the VSSE (S.epidermidis 22) didn't showed any cell wall thickening.

دراسة مقارنة للكشف عن الجيارديا لامبليا والطفيليات المصاحبة لها بين الاطفال في مدينة كركوك باستخدام بعض التقانات المختبرية == A Comparative Study For Detecting Giardia Lamblia And Associated Intestinal Parasites Among Children In Kirkuk City By Using Some Laboratory Methods

Author name: مها اسماعيل مصطفى الجبوري
Supervisor name: يحيى جرجيس سلمان
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: English
University location: Kirkuk
First pages:
Abstract: The current study had been carried out from January 2013 to July 2013 in medical laboratory researches - Kirkuk Faculty of Medicine. A total of 310 stool samples have been collected from children suffering from liquid diarrhea, their ages are from below one year to 12 years; six different laboratory diagnostic methods have been applied for detecting Giardia lamblia and other intestinal parasites. For microscopy diagnosis; direct wet double prepartions, zinc sulphate flotation are used. While immunological methods involve; Enzyme Linked Immuno - Sorbent Assay, corpo - antigen (ELISA), Direct Fluorescent Assay (DFA) and Lateral immune - chromatography assay (Triage panel). Giardia genome amplification is done using conventional Polymerase Chain Reaction (PCR) single step procedure by using of mixed primers of Giardia assemblages A1, A2 and B. The total rate of intestinal parasitic infection is 51.93 % distributed in 161 stool samples. This rate involve high frequency of protozoan infection in 132 (42.58 %) compare to 29 (9.35 %) for helminthes, P<0.05. More common intestinal protozoan parasites were Giardia lamblia 63(20.32 %) followed by Blastocyst hominis, Cryptosporidium parvum, Entamoeba coli, Entamoeba histolytica, Iodomoeba butschili, Endolimax nana and Balantidium coli with the rates : 6.77%, 6.45%, 4.18 %, 2.58 %, 1.29 %, 0.64 % and 0.32 % receptively. Concerning intestinal helminthic infection, high number and rate 23(7.41 %) was with Hymenolepis nana is compare with 1(0.32 %) record for Ancylostoma duodenale.According to gender high rate of giardiasis is recorded among males than in females, conversely to high rate of other intestinal parasites among female than in males P<0.05. Statistically relationship between Giardia distribution and ages are not significant P>0.05. Giardia co - existence are highly detected with Balstocystis hominis, and Cryptosporidium parvum total Giardia mixed parasitic infection rate 8.06 % is lower than12.23 %of pure Giardia lamblia infection, P>0.05. Regarding Giardia lamblia detecting according to laboratory methods; high rate of giardiasis 22.29% is reported by using PCR technique, followed by 20, 23 % by using direct wet preparation technique, P<0.05.The efficacy of laboratory methods for detecting Giardia stages are reduced; the following rates 19.35%, 19.03%, 17.74% and 14.51% obtained by using ELISA, DFA, flotationand lateral immuno - chromatography assay (Triage) respectively, P<0.05. Also statistical analysis reveals significance of PCR sensitivity, specificity, and accuracy in detecting giardiasis than other laboratory methods. Negative predictive values NPV in relation to type of laboratory methods are high, but statistically they are not significant, controversy to positive predictive value PPV that are significant. The efficacy of Triage panel is high for detecting giardiasis 14.45 % as compare with 4.5 % and 3.5 % for detecting Cryptosporidium parvum oocysts and Entamoeba histolytica respectively P<0.05 %. Considering the application of double direct wet preparations, the results of using this method are beneficial for detecting protozoan and helminthic parasites. While the using of zinc sulphate flotation technique reveal fluctuated results in spite of significant statistical analysis P<0.05. The employee of five laboratory methods for detecting the oocysts of Cryptosporidum parvum; the following rates 6.45%, 5.48%, 4.83%, 4.51% and 3.22% are recorded with the usage of DFA, direct microscopy, modified Ziehl - Neelsen method, Traige panel and flotation method respectively, P>0.05. Giardia lamblia DNA extraction from 80 stool samples that amplified using Giardia gene loci K725, reveal 66 samples positivity, pure Giardia lamblia genomic mass mean is 437.56 bps, with 1.705 % of genome purity and the extension of genomes range from 280 to750bps.While 23 of mixed Giardia plus other protozoan parasites, the mean of gemonic mass is 439.89 bps with genome purity mean 1.56 %. Amplification of Giardia genome mixed with helminthes reveal 443.33 bps of genomic mass mean and 1.49 % genome purity mean. In general the all genomic mass of Giardia lamblia (Pure and mixed infection) is 440.26 bps and purity mean 1.54 % P>0.05.PCR amplification in stool sample exert that Giardia lamblia genomes are mixed of human and animal type.

دراسة انتشار الحيوانات الابتدائية الرئيسية المسببة للاسهال بين المرضى باستخدام الطرائق المجهرية والجزيئية في محافظة بابل == Prevalence Study For Main Protozoa Diarrheal Agents Among Patients By Using Microscopically And Molecular Methods In Babylon Province

Author name: ميس كاظم عليوي
Supervisor name: احمد خضير عبيس الحميري
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: English
University location: Babylon
First pages:
Abstract: اجريت الدراسة الحالية خلال المدة من تشرين الاول 2014 الى شهر شباط 2015 حيث تضمنت هذه الدراسة فحص 987 عينة براز (اطفال وبالغين, ذكورو اناث) بطريقة الفحص المجهري المباشر و96 عينة موجبة فحصت عن طريق تقنية تفاعل سلسلة متعدد البلمرة. للمرضى المصابين بالاسهال و| The current study during period was conducted from October 2014 till February 2015 examination of 987 stool samples for direct smear method (Lugol's Iodine, Normal Saline (0.9%) for detection of the following parasites G.lamblia, E.histolytica while using floatation methods and Ziehl - Neelsen method (Malachite green) for detecting the Cryptosporidium spp. by using light microscope) and 96 positive samples from these samples examined by polymerase chain reaction technique PCR. For patients infected with diarrhea (children and adults, male and female) who attended to Babylon maternity and children hospital and specialized Marjan Hospital for Internal and Cardiac Diseases in the Babylon province as well as primary health care and private clinics. The age ranges from(Less than one year - 31and more).The current study showed the rate of infection with parasites that causative of diarrhea47.3% (E.histolytica, G.lamblia and Cryptosporidium spp.) was 26.4%, 17.9% and 3.7%, respectively.They were examined by a direct smear method to detect the trophozoites, cyst and oocyst phases of these parasites. The highest rate of infection by microscopic examination was in the rural area 67.2% in comparing with city that was 32.9%. Also the highest rate of infection among males was 51.5% in comparing with females 41.2%. It has been recorded that the higher rate of infection was 76.1% in the age group (16 - 20) years while the lower infection rate was in the age group (26 - 30) years that was 22.8%. It has been observed significant differences in infection rates at the (P ? 0.05). The present study recorded difference in the rates of parasitic infections according to the presence of animals in houses, the high rates of infection where with those have animals in their houses 51.2% while the lowest rates of infection where with those not have animals in their houses 38.9%.It has been observed significant differences in infection rates at the (P ? 0.05). This study reveals a difference in parasitic infections rates, according to the level of education the head of household, and found that the highest rate of infection for those with the head of the family is non - educated or illiterate 48.3%, while the rate of infection was declined with head of the family who get primary education level 46.2%. It had recorded the highest infection rate in October 63.8%, while the lowest percentage was in February 27. 6%. It has been observed significant differences in infection rates at the (P ? 0.05). In the present study Polymerase chain reaction (PCR) is used to 96 positive results in direct smear methods to detection the main parasitic diarrhea agent. It had recorded total of infection rate of 43.4% (31.3%, 28.1% and 2.1%, respectively). Depending on PCR technique showed the highest rates of infection was in the male 36.7% while the lowest rates of infection in females 30.6%. It had recorded the highest rate of infection in the rural areas 45.3% in comparing rate of infection in the urban areas 25.9%. The present study recorded the highest rates of infection were in the (16 - 20years) age group 46.2%, while the decline in the rates of infection was in the (21 - 25years) age group 16.7%. Also it showed the highest rates of infection were in the presence of animals in the houses 36.1% while the lowest rate of infection where there were no animals 31.4%.It has been observed significant differences in infection rates at the (P ? 0.05). The study found that the highest rates of infection for those with the head of the family is non - educated or illiterate 38.9%, while the rate of infection declined with head of the family who got Academic education level 33.3%. Also it had recorded the highest rate of infection in November (2014) 42.1%, while the lowest rates percentage was in December (2014) 23.1%. It has been observed significant differences in infection rates at the (P ? 0.05). Through the current study it was concluded that the prevalence of the parasites that cause diarrhea in the Babylon province are very highly when detection microscopic examination and PCR method, comparison with previous studies and rural area highest rates of infection from urban area.

تاثير المستخلص الكحولي لنبات الخباز (Malva sylvestris ) في فسلجة ونسجية الكبد والخصية في ذكور الفئران البيض == Effect of Alcoholic Extract of Mallow (Malva Sylvestris) On Physiology And Histology Liver And Testis In Albino Male Mice

Author name: بسمة علي جاسم
Supervisor name: مختار خميس محمد سعيد
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Baghdad
First pages:
Abstract: صممت هذه الدراسة للتعرف على مدى تاثير المستخلص الكحولي الخام لنبات الخباز Malva Sylvestris في الكبد والخصى لذكور الفئران البيض نسجيا, وكذلك فسلجيامن خلال دراسة مستوى انزيمي Glutamate - oxaloactate - transaminase (GOT) وGlutamate - pyruvate - transaminase( | This study was designed to investigate the effects of crude alcoholic extract of Malva sylvestris on the histology of liver and testes in male albino mice, as well as levels of the GOT, GPT enzymes and testosterone hormone. Seventy two adult male albino mice were used in this study. The animals were randomly divided into four main groups, the first group was given distilled water (control), while the other three groups were given (100, 300, 500 mg /kg) of crude extract. These main groups were subdivided into three subgroups, according to the period of treatment (2, 4, 6, 8)weeks. Blood samples were collected every two weeks for physiological study, and samples of the liver and testes were collected for histological study. Treating with crude alcoholic of Malva sylvestris led to no significant increase in the mean of the body weight except the concentration (500 mg /kg). There was significantly increase in the liver weight particularly in(500 mg /kg) for eight weeks of exposure, also there was significantly increase in the average of the testes weights treated with Malva sylvestris extract specially in (300, 500 mg /kg) for all periods of treatment compared with control group. Malva sylvestris extract coused significantly decrease in both GOT, GPT. there fore the value of GOT, GPT considers as concentration - period exposure dependent the highest value of GPT was (300 mg /kg) for two weeks (2. 25±228. 04) IU/L compared with control group (3. 03±226. 02) IU/L. and the lowest value of GPT was (500 mg /kg) for eight weeks (4. 52±192. 27)IU/L compared with control group (4. 06±208. 77) IU/L the highest value of GOT in (100 mg /kg) reached (4. 10±63. 00) IU/L for two weeks compared with control group (1. 25±71. 23) IU/L. the lowest value was (2. 77±49. 83) IU/L in (500 mg /kg) with eight weeks comparison with control group (1. 18±69. 02) IU/L. all concentration of Malva sylvestris extract made positive changes in the value of testosterone with all periods of exposure particularly in (300 mg /kg) compared with control group. There were many histopathological alterations appeared in the liver tissue among treated groups which caused by the effects of Malva sylvestris extract represented by congestion, disarrangement of hepatic cords, beginning of necrosis of hepatocytes, infiltration of leukocytes, narrowing of sinusoids, increase the number of binuclei cells, degeneration, appearance of giant cells, all these changes were reversible. Malva sylvestris extract made beneficial histopathological changes in the testes tissue in all treated groups like increase in numbers of ledyig cells specially concentration (500 mg /kg) reached (11.37± 0.80) IU/L with two weeks comparison with control group(5.38 ±0.34) IU/L, and increase in the thickness of the walls of seminiferous tubules specially concentration (500 mg /kg) reached(29.46± 1.39) IU/L with compared with control group(20.52±2.40) IU/L. In addition the role of extract in significant decline in the diameters of seminiferous tubules in all treated groups compared with control group

دراسة العلاقة بين فقدان الجينين الـ GSTM1 وGSTT1 والاصابة بمرض الربو القصبي في محافظة ذي قار == Study The Relationship Between The Null Genotype of GSTM1, GSTT1 Genes And Bronchial Asthma In Thi - Qar Province

Author name: نـور عمران عبد الكريم الغـزي
Supervisor name: حسن ريسان الركابي | عدنان عيسى البدران
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: تم تصميم هذه الدراسة للتحقق من دور جينات الـ Glutathione - S - Transferase (Mu - 1) gene و(Theta) - 1 Glutathione - S - Transferase ومدى مساهمتها بخطر الاصابة بمرض الربو القصبي في محافظة ذي قار. حيث شملت (105) شخص مصاب بالربو باعمار من (20 - 69) سنة كان | This study has been designed to investigate the roles of the gens GSTMI and GSTTI the extent to which they contribute in asthma at Thi - Qar governorate. The study has been conducted on (105) patients with asthma whose ages from (20) to (69) years. (20) of them are males and (85) are females, and (110) are healthy with no infection of asthma or any sickness of respiratory system as a control group of the same ages, (60) of these are males and (50) are females. Blood samples have been collected from both groups in ampoules with EDTA and stored under temperature ( - 20) until they are used in getting the DNA from the groups. The genes GSTTI, GSTMI, and Albumin have been enlarged as internal control employing Polymerase chain Reaction (PCR). Some dangerous aspects of this disease have been investigated, the results showed that the disease in the urban areas (85. 71 %) higher than it rural areas, and Asthma among females is (80. 95%) higher than males. The results also indicated that asthma in ages (50 - 59) is more than other categories; it is (34.28%). The majority of patients are smokers with (63.80%). The study revealed that (73.33 %) of the patients have a family history with the disease while (26.66 %) have not. Statistical analysis showed that the loss of the gene GSTM1 is about three times in the patients more than the case in the control group. (OR=2.90) , while the loss of the gene GSTT1 is five fold more than it compared to the control group (OR= 5.55). The results indicated a significant effect of the loss of the two genes in having asthma in the urban and rural areas, it is six times and a half larger in the urban areas compared to the control group of the same area. Asthma probability is ten times more in the case of the loss of both genes in the rural areas compared to the control group in the same area. And no significant difference has been discovered when gene patterns of patients in both areas. The results also indicated that the probability of having asthma in male patients is two times and a half as many as it in the females when the gene GSTM1 is lost compared to the control group. While the same probability is eleven times in the case of the loss of both genes in the females as many as it the control group. The loss of Missing the gene GSTT1 is three times in the male patients and five times in the females compared to the two control groups. The results indicated significant differences when gene patterns of males and females are compared in the case of the loss of both genes. The statistical analysis indicated that the loss of both genes in the smoker patients contributes 17 fold in the probability of having the disease compared to the control group. While the probability increases four times approximately in the nonsmokers when the gene GSTM1 is lost compared to the control group (OR=3.77; 95% C1=1.69 - 8.407). No difference has been noticed in the gene patterns of the smoker and nonsmoker patients. The results indicated that the loss of both genes has been in all the age categories. No significant differences have been noticed among the gene patterns between the patients of less and those who are more than 50 years. Asthma probability has been found 7.77 times more in the case of the loss of both genes in the patients who have a family disease history compared with the control group. The asthma probability is twice when the gene GSTM1 is lost in those patients who do not have a family history disease compared to the control group. Upon comparing the gene patterns of patients according to the family disease history, there has been an effect of the loss of both genes and having a family disease history on the probability of asthma, it is almost six times when both genes are lost. The results indicated that the probability increases three times in the loss of the gene GSTT1 in the educated patients compared to control group. The probability also increases seven times when both genes are lost in the uneducated patients compared to the control group. When comparing the gene patterns of patients according to levels of education, significant differences between the educated an uneducated have been approximately twice in the loss of the gene GSTT1.
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