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التشخيص الجزيئي للخمائر والاعفان المرافقة لمخلفات الطيور وتقييم كفاءة بعض المستخلصات النباتية ضد خميرة Cryptococcus == Molecular Identification of Molds And Yeast Associated To Birds Wast And Evalution Efficiency of Some Plant Extract Against Cryptococcus
Author name:
رسل عصام علي الظاهر
Supervisor name:
زيدان خليف عمران المعموري
General topic:
Biology
Specific topic:
Life Science
Degree:
Master
University:
University of Babylon - College Of Science For Girls - Department Of Biology
Language:
Arabic
University location:
Babylon
First pages:
24T2830 - p.pdf
Abstract:
تضمنت الدراسة الحالية عزل وتشخيص الاعفان والخمائر المرافقة لمخلفات الطيور باستخدام بعض التقنيات المظهرية والجزيئية. كما تم اختبار فعالية المستخلصات المائية لنبات وعرك لوية والقرنفل والبابونج في تثبيط نمو خميرة Cryptococcus neoformans.تم جمع 190 عينة من م | The present study aimed to isolation and diagnosis the molds and yeast from birds extract by use some of phenotype and molecular techinces. As well as the aqeous plant extracts of clove and Germin Chamamilla and east indian scrow tree.to inhibition Cryptococcus neoformans growth there is about 191 isolate was been collected from Babil and Karbala included 30 isolate (10 isolate hand printing and 10 isolate from ambiend air of bird brought zone, and 10 isolate from cough of men they are dealing with birds in pitry dish containing SDA media. The other 161 isolate was been collected from the wet and dry bird extract from the different environment.Culture method was used in the diagnosis as CHROMagar media to diagnosis Candida spp. Also used the haemolysis test of Macrorhabdus ornithogaster yeast and phospholipase and Urease test of Cryptococcus neoformans yeast.And used the molecular methods as DNA extraction and used PCR technice by use universal and specific primers.Results of analysis isolation and diagnosis showed that the maximum of present appearance was for Candida spp. 45.34% and molds Rhizopus sp. appearance percent 27.32% and frequency percent 28.21% and 3.65% consequently M. ornithogaster appearance percent 4.96% and frequency percent 35.77% while the other molds and yeast have appearance percent between 0.62 - 19.25% and frequency percent between 0.07 - 19.75% and the result of CHROMagar for Candida gives different color C.albicans was green color and C.krusei was pinki color.Cryptococcus showed apositive result in phospholipase and Urease test. haemolysis test of Macrorhabdus yeast was positive the hot aqeous plant extracts of clove and Germin Chamamilla and east indian scrow tree have the activity to inhibit the growth of Cryptococcus neoformans, but the more activity was east indian scrow tree with concentration 4%.The pair primer CAP60 succesful to diagnose Cryptococcus neoformans It was 603bp and primer AGY1\Sm2 successful to diagnose Macrorhabdus yeast
