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دراسة تشخيصية وجزيئية لطفيلي الجيارديا المعوية Giardia intestinalis لدى الاطفال المصابين بالاسهال في محافظة القادسية == Diagnostic And Molecular Study of Giardia Intestinalis In Children Infected With Diarrhea In Al - Qadisiya Province
Author name:
لبنى عبد القادر خنياب الابراهيمي
Supervisor name:
هادي مدلول حمزة الميالي
General topic:
Biology
Specific topic:
Microbiology - Parasites
Degree:
Master
University:
University of Al-Qadisiyah - Faculty Of Education - Department Of Biology
Language:
Arabic
University location:
Qadisiyah
First pages:
24T2822 - p.pdf
Abstract:
تضمنت الدراسة الحالية فحص 926 عينة غائط للاطفال المصابين بالاسهال والمراجعين لمستشفى الديوانية التعليمي ومستشفى الولادة والاطفال، وبعض المراكز الصحية والمختبرات الاهلية في محافظة القادسية خلال المدة ما بين 1/11/2012 الى 30/6/2013 وللفئات العمرية من ?1 - 1 | The current study included examination of 926 stool samples of children with diarrhea who attended to didactic hospital in Al - Diwaniya. The maternity hospital, some health centers and competent laboratories in Al - Qadisiya province during the period from 1/11/2012 to 30/6/2013 and for age range of ? 1 - 12 year exclusively. The current study showed the rate of total infection of Giardia parasite was 5.61% (52 of 926 samples). They were examined by direct wet smear method and floatation method by using light microscope to detect the trophozoite and cyst phases of parasite. It was found in numerous stool samples. The results of samples showed that the infection in males was higher than in females 6.12% and 5.11 % respectively. The age range 2 - 4 years recorded highest incidence of infection and the age range 8 - 10 years recorded the lowest incidence of infection 13.87% and 1.52 % respectively. Also it was recorded higher infection rate in June and the lower infection rate in January 10.37% and 1.92% respectively. It was recorded higher infection rate among the rural population in comparing with urban population 7.07% and 4.45 % respectively. statistical analysis results show a significant differences at(P?0.05). In the present study, Real - Time polymerase chain reaction (PCR) was used to detect Giardia parasite infection in microscope positive examined samples where it had record total Infection rate of 73.07% (38 of 52). Statistical analysis of survey results after depending on PCR technique showed significant differences in infection among the age range. The age range 2 - 4 years had recorded the higher infection rate 83.33% while there are no significant differences of infection according sex.It reached in males to 75% in comparing with females 70.83 %, also it did not show significant differences in infection rate according to living areas.It had reached in rural areas to 75.86 % in comparing with urban areas 69.56%. In addition, polymerase chain reaction technique was used (PCR) and restriction fragment length polymorphism (RFLP - PCR) to determine genotypes of Giardia intestinalis parasite for 20 case from diarrhea positive cases of children in Al - Qadisiya province, by amplifying gene (gdh) glutamate dehydrogenase gene by using specialists primer. They are GDHiF and GDHiR It got the amplification results successfully in all stool samples of 20 and positive examined Real - Time PCR were it had determined the existence of gdh gene of molecular weight of 432bp in all those samples. The study showed that 7 of the previous samples belong to genotype A (35%) and 13 samples belong to genotype B (65%).It showed that all samples of genotype A belong to secondary genotype AII in rate 100%, while samples of genotype B belong to two secondary genotypes, they are BIII (61.53%) and BIV (38.46%). The study showed the appearance of genotype A in the age range under six years, while genotype B in all age range approximately. The distribution of genotypes according to sex and housing it had record both genotypes appeared in both sex with different rate genotype B was more appearance than genotype A in males than females. According to the nature of housing secondary genotype AII had appeared in rate 70% in urban areas, while genotype B with its secondary different genotypes in both areas urban and rural, but with higher rate in rural areas (100 % and 30 % respectively). We had concluded from the current study that there is a close relationship between infection, the sex, age range, nature of settlment and monthly distribution.There are two basic geno types in province, they are A and B, for each of them there aresecondary genotypes AII, BIII, BIV.
