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دراسة تصنيفية لبعض مراتب الجنس Ranunculus L. (Ranunculaceae في العراق A Taxonomic Study of Some Taxa of The Genus Ranunculus L. (Ranunculaceae) In Iraq

المستخلص: “The current research dealed with fourteen taxa belonging to the genus Ranunculus L., Family Ranunculaceae that grown in Iraq. The study included the gross general morphological and micromorphological characters, the Pollen grains also were studied and the chemical compounds (Flavonoids), Ecological and geographical distribution and taxonomic treatment have been done. Also botanical key was puted to separate the Taxa”.“General survey for the Phytogeographical districts where the taxa spread in, and the result was a large crowds of samples, and new locations of taxa distribution were recorded. The specimens in the Iraqi herbaria were studied after ensuring that it was correctly identified”. “Comparative morphological study was undertaken to all plants parts of taxa including the roots, leaves, stipules, flowering parts and fruiting parts. Charts, diagrams, tables were drawn and accompanying with natural images for different morphological characteristics, as well as the field images that are important for the study. This study showed that all the taxa of the genus were herbs that are annual or perennial. There was agreat important for the roots, stems and there Indumentum, the presence orabsence of stipules, shape and size and margin of basal leaves and cauline leaves and leafy bract, the shape of sepals and their numbers and their Indumentum, the numbers and shape of Petals and their colors, the number of Stamens and the colors of Anthers, the shape of ovary and their indumentum, presence or absence of style as it was very useful for the taxonomic identification and separation of the studied taxa. As well as the fruiting parts including the length of fruiting peduncle, the fruiting receptacle, the fruiting head and its shapes and their dimensions, the number of Achene Fruitlets and their sizes, colors and Beak shape. Also the surface configuration of the fruitlets Surface has been studied by (SEM) as it wae either hairy or tuberculate - hairy or Transversely rugose or muricate or reticulate or reticulate - minutly rugose or granular or granular - hairy or granular - hairy tuberculate or spinulose”.“The study of the micro - morphological characters of hairs, leaves epidermal cells and the stomatal complex, have a taxonomic importance of supporting the morphological characters in separation and identification of the genus taxa”.“The study show that the pollen grains have a taxonomic importance because of their difference in shape, dimensions, the wall thickness and their ornamentation. It was either apolar which was spheroidal and pantoporate, or isopolar which has different shapes in the equatorial view it was spheroidal, subprolate and oblate - spheroidal, it was either 3 - colpate, 4 - colpate or 5 - colpat.the colpate where either zonocolpate or syncolpate in the form of parasyncolpate. The ornamentation were verucate, echinateand scabrate”. “The chemical study also has an importance in separation of the genus taxa, six flavonoid compounds were diagnosed by using” HPLC wich are Kaempferol, Vitexin, Orientin, Quercetin, Iso - vitexin and Iso - orientin. According to the presence or absence of those structures cluster analysis where done for the results of the flavonoid compounds and the taxa where divided into groups that reveal the chemical relationships between them.dendogram where performed which also show the chemical relationships between the taxa by using UPGMA method and NTSYS program issue 21. the study shows that the presence and distribution of those compounds vary according to the variation in the plant distribution the study shows that some of the taxa have “the same compounds which enhance its belonging to the Ranunculus genus”. “Through field surveys and information installed on the herbarium samples, the taxa where distributed on their growth areas, and maps have been drawn for taxa distribution in the Iraqi natural geographical. Most of the taxa were distributed in N. and N.E of Iraq regions districts especially in MAM, MSU, MRO, and FBF districts. We notice that MSU district has a great number of the plants under the study as we found there are all the taxa accept one”. This study helps to detect distribution areas for some taxa that have been not mentioned in the Iraqi flora or other records which are R.kotschyi and R.trichophyllus var.trichophyllus. Also the study records distribution for the new species R.chius in MAM district which hasn't been mentioned in the Iraqi flora in this district before. Finally all the taxa were classified and the botanical key was done to separate them.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: مازن نواف عبود العاني
اسم المؤلف: علي طالب محمد الطائي

البدانة عامل خطورة للاصابة بامراض القلب التاجية Obesity As A Risk Factor For Coronary Heart Disease

المستخلص: هناك مفهوم متنام يشير الى زيادة انتشار البدانة بين مرضى القلب التاجية Coronary heart disease (CHD), مما له الاثر الكبير في نشوء المرض ومضاعفاته, اذ يشابه عمل الخلية الدهنية adipocyteعضو الافراز الداخلي endocrine organ.ولمعرفة العلاقة بين البدانة وامراض | There is a growing concept that refers to the increased prevalence of obesity among patients with coronary heart disease. This has significant impact on the emergence of the disease and its complications as the work of the adipocyte resembles the work of the endocrine organ. This study comes to know the relationship between obesity and heart disease. To accomplish this, about 5cm3 of venous blood has been collected from 240 patients of coronary heart disease who are admitted to the resuscitation unit in Baquba Teaching hospital for the period 1st of December 2014 to1st of January 2015. The range of the patients' age is between 22 - 90 years old. The sample has been divided into three groups in accordance with body mass index and according to the classification of the American Institute. These groups are : natural weight from 18.5 - 24.9kg/m2, overweight from 25.00 - 29.9 kg/m2 and obesity30.00 kg/m2. The result shows that there is a significant rise in the fasting blood sugar, the total protein in serum and the systolic pressure with the rise of the body mass. While the level of the cholesterol, triacylgcerol and the remnant lipoprotein of the patient has not been affected with the different of the body mass. The patients with natural body mass index show significant rise in the total and direct bilirubin levels as compared with other groups with no significant differences among the groups in the levels of uric acid, urea, creatine, and phosphorus and iron ionic. We conclude that there is a correlation between the body mass index and the systolic and diastolic blood pressure.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان - الفسلجة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: حميد محمود مجيد
اسم المؤلف: نور غسان كاظم الدليمي

دراسة بعض المؤشرات المناعية لدى بعض المرضى المصابين بداء السكري النوع الثاني في محافظة ديالى A Study Some Immunological Parameters In Some Patients of Diabetes Mellitus Type Two In Diyala Province

المستخلص: Type II diabetes mellitus is achronic diseas characterized by hyperglycemia resulting from defects in insulin secretion and insulin action or both and tissue resistante to insuline. This study was conducted in the Diyala province for the Type II diabetes patients which lasted for the period from beginning October 4102 to the end of May 4102 which aimed to evaluate some immunological indicators for patients infected with diabetes type two which included { assessment of the humoral immune by measuring the level of immunoglobulin (IgM, IgG) and the level of complement protein (C3, C2) by using the single radial immune diffusion assay and measuring the C - reactive protein (CRP) and determine the levels of Interlukin (IL - 01, IL - 01) by ELISA}. Atotal of (011) ablood sample was collected from patients with diabetes type two after diagnosis by a physician specialist in the clinic consulting in Baquba teaching hospital according to the criteria adopted by the World Health Organization for the diagnosis of diabetes type two, has been the number of males (24) percentage (20.11)% and the number of femals (22) percentage (21.02)% within the range of my age (32 - 11) years and collected (11) of blood sample from healthy volunteers ostensibly have been accredited as a group control, has been the number of males (32) percentage (21.33)% and the number of females is (42) percentage (20.14)% within the range of my age (31 - 44) years.Results of the study showed that the incidence of the disease rate a mong females (21.02)% than males (20.11)% with no significant difference statistically between the two groups of study for the sex, has found that age group (24 - 11, 11<) years recorded a high rate of disease (33.14)% and(32.12)% respectively while the age group (31 - 21) years percentage (1.11)% recorded lowest rate of disease whith high significant difference was noticed between the two groups.Furthermore results of the study showed low levels of immunoglobulin (IgM, IgG) in patients compared to the control group where found that the level IgM in patients (043.20124.22) mg/dl compared to the control group (434.24121.24) mg/dl and that the level of IgG in patients (0124.2110123.21) mg/dl compared to the control group (0103.32 ± 0442.14) mg/dl the results also showed low levels of complement proteins C3, C2 in patients compared to the control group it was found that the level of C3 in patients (01.00 ±31.14) mg/dl compared to the control group (030.02 ± 01.24) mg/dl and the level of C2 in patients (02.31 ± 4.11) mg/dl compared to the control group (20.231 4.13) mg/dl, and the results showed that the positivity of C - reactive protein in patients was (41.24)% compared to the control group was (1)% The results of the current study showed high level of IL - 01 in patients (410.24 ± 011.40) pg/ml compared to the control group (14.11132.43) pg/ml and the level of IL - 01 the results showed alow level of the IL - 01 in patients (13.13 ± 20.01) pg/ml compared to the control group (43.04 ± 40.03) pg/ml, inaddition to the study result showed agreat significant correlation between complement proteins (C3, C2) and the humoral immune (immunoglobulin IgM, IgG) as well as acorrelation between cellular immunity (IL - 01)and (IgM, C3).
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: محمد عبد الدايم صالح
اسم المؤلف: ريم قاسم محمد الحديدي

تقييم فعالية المستخلص المائي والكحولي لنبات الصبار Aloevera في الاصابة التجريبية لطفيلي Eimeria tenella لدى فروج اللحم Evaluationof Alcoholic Andwateryextracts Efficacyofaloe Veraon Experimental Infectedbroilerwitheimeriatenella

المستخلص: اجريت الدراسة الحالية على (216) فرخ دجاج نوع Rose 308مصاب بطفيلي Emiria tenella خلال المدة من شهر نيسان 2013 الى شهر تشرين الاول 2013, وقد تم في الدراسة الحالية تحديد بعـــــــــض المؤشــــــــــــــرات الدمــــوية (الخلايا المتغايرة /الخلايا اللمفاوية HL | This study was conducted to (216) broilers chickens (Rose 308 Trade) infected parasite with Eimeria tenella performed form April 2013 till October 2013, has been in the current study to identify some indicators of blood(Heterophils/ Lymphocytes, White Blood Cell, Red Blood Cell, Packed Cell Volume, Hemoglobin) and biochemistry (Glucose, cholesterol and total protein) and productive performance (Weight increase, Food conservation, Cansumbtion and Body weight befor slugtery) , We note at the parasite has infected chicks were used three extracts of Aloe vera (alcoholic and hot water and cold water), dosage chicks were to demonstrate the effect of different concentrations of these extracts on the productivity and physiological traits mentioned above. Then the experiment was designed, which include the following : 1 - The first group (control group) included 36 chicks and was given food with no anti - coccidial. 2 - The second group include 36 chicks in duplicate was infected but did not treated.3 - The third group inclouded 36 chicks in duplicate was given extract of Aloe vera Alcoholic ethyl in adose of (0.5 - 1.5 - 2)gm per mm body weight.4 - The fourth group inclouded 36 chicks in duplicate was given extract of Aloe vera Cold water in adose of (0.5 - 1.5 - 2)gm per mm body weight.5 - The fifth group inclouded 36 chicks in duplicate was given extract of Aloe vera Hot water in adose of (0.5 - 1.5 - 2)gm per mm body weight.6 - The sixth group include 36 chicks in duplicate was given Vetacox(trade Mark)that had both Sulphadimidin 80 gm and Diverdin8 gm.Results showed the following : - 1 - The alcoholic extract of Aloe vera at concentration (0.5, 1.5, 2) g/ml after an infection in the fifth week revealed alittle effect on the H/L when compared with eighth week of infection. also this extract lead to increase, the rate of (PCV), (Hb) , and (RBC) while lead to decrease the rate of (WBC) after eighth week of infection at concentration (2)g/ml. Also this extract lead to increase the rate of cholesterol, total protein and the rate of weight increased in the eighth week, while the amount of feed intake and feed conversion showed alittle effect, as well as the body weight befors slaughter has astrong effect at concentration(2)g/ml in the eighth week of infection. 2 - The cold and hot aquatic extract of Aloe vera at concentration(2)g/ml lead to decrease the ratio of H/L and increase the rate of (PCV), (Hb) and (RBC) after the infection in the eighth week, also lead to decreas the rate of (WBC) and increase the rate of cholesterol, total protein, the rate of weight increased, the amount of feed intake and the body weight before slaughter at concentration (2)g/ml in the eighth week of infection. Also recorded abetter efficiency of feed conrersion at concentration (2)g/ml in the eighth week of infection.
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: هيثم محمد حمادي العوادي
اسم المؤلف: زهراء حاتم حميدي عبود الكعبي

دراسة تصنيفية حياتية مقارنة لمراتب من الجنس Onobrychis Mill. (Fabaceae) في العراق

المستخلص: استــهدف البــحث الــحالي دراســة تصــنــيفية مقارنة لاحدى عشر مرتبة تصنيفية تابعة للجنس Onobrychis Mill. من العائلة الفراشية (البقولية) Papilionaceae النامية بريا في العراق وهي O. acaulis وO. caput - galli وO. carduchorum وO. crista - galli بضربيه O. cri | A comparative systematic study was carried out for eleven taxa belong to the genus Onobrychis Mill., Family Papilionaceae grown widely in Iraq, and they are O. acaulis, O. caput - galli, O. carduchorum, O. crista - galli with two varieties O. crista - galli var. crista - galli and O. crista - galli var. trilophocarpa, O. galegifolia, O. haussknechtii, O. kotschyana, O. megataphros, O. ptolemaica, O. schahuensis. The Study included the gross general Morphological characters and Micromorphological characters, also the Pollen grains studied and Anatomy, Chemistry, Enviroment and Geographical distribution and Taxonomic treatment, also botanical key was putted to separate the taxa. General survey for the Phytogeographical districts where the taxa of genus distributed, in order to detect the distribution of the studied taxa of the genus Onobrychis. Therefore many collections obtained and new locations of taxa distribution were recorded, the specimens studied, and correct the mistake naming specimens were identified and reidentified some miss identified specimens. A comparative morphological study was undertaken to all taxa plants parts include the root, stems, leaves, stipules, flowering parts and fruiting parts and drew charts diagrams, tables and accompanying, with natural images for different morphology characteristics, as well as the field images. This study indicates that the characters of Leaf, Inflorescence, Flowers and Fruit were collectively contributed in the separation and identification of taxa. The pollen grains have a taxonomic importance because their different shape, dimensions of polar and equilateral view and wall thickness. The anatomical study showed the importance of Taxonomic support to the morphological characters in the diagnosis and isolation different taxa, and this study showed different in the anatomical characters of leaf include the characters of Epidermis, Stomata and the characters of a cross sections in stems. The current study showed that the trichomes were very important taxonomic in isolate different taxa because their variation in density, dimensions and the shape of epidermis cells that the trichomes arise from them and dimensions of the epidermal cells and the nature of their walls. The current study identified nineteen protein - amino acids by using High - Performance Liquid Chromatography (HPLC) and the distribution of these compound was variable among various taxa of the genus and that was useful in isolation different taxa. and the aliphatic amino acids, Glutamic acid, Glycine, Serine, Arginine, Alanine, Tyrosine, Valine, Methionine, Isoleucine and Lysine had a wide presence in the studied taxa, which means that they belong to the same genus. The ecology and geographic distribution of the taxa were studied using area survey and information of herbaria specimens, maps have been drawn for taxa distribution in the Iraqi natural geographical provinces. At last, a taxonomic treatment has been carried out for the taxa of the genus Onobrychis, as well as a detailed key to isolate them.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عذية ناهي سلمان المشهداني
اسم المؤلف: زينب جواد عامر

تاثير المياه الممغنطة والبنزل ادنين ومستحضر السماد الطحلبي في نمو نبات الجزر Daucus carota L. Effect of Irrigation Water Magnetic, Benzyladenine And Algal Fertilizer Preparation On Growth of Carrot Plant Daucus Carota L

المستخلص: نفذت التجربة باستعمال الاصص خلال الموسم الشتوي للعام (2012 - 2013) م في تربة مزيجية وتحت الظروف البيئية الطبيعية في قسم علوم الحياة/ كلية التربية/ جامعة القادسية, لمعرفة تاثير مياه السقي الممغنطة والرش بتراكيز من البنزل ادنين ومستحضر السماد الطحلبي في ال | An experiment was conducted in the winter season of the year (2012 - 2013) in loam soil pots, in Biology department/ College of education/ Al - Qdisiya university, to find out effect of irrigation water magnetic, spryer of benzyl adenine concentrations and algal fertilizer on growth, mineral content and carotenoids to carrot plant (Daucus carota L.).The design of the experiment was randomized complete blocks (RCBD) in a factorial arrangement with three factors. The first factor was four intensities of magnetic to irrigation water (0, 500, 750 and 1000) gauss, the second factor was three concentration of benzyl adenine (0, 50 and 100) mg.L - 1 and third factor was three concentration of algal fertilizer (0, 1.5, and 3) ml.L - 1, with three replicates. The treatments means were compared by using averages revised least significant difference (RLSD) at 0.05 probability level.The following parameters were measured during the study plant height, leaf area, leaves number, vegetative fresh and dry weight, root dry weight, Leaves content of chlorophyll (a, b and total chlorophyll), carotenoids, Baicalin and Pycogenul, in addition to percentage of NPK, total protein and Fe (µg. g - 1). Results showed : 1 - The use of magnetic water by 1000 gauss were increase in plant height, leaf area, leaves number, vegetative fresh and dry weight, root dry weight, Leaves content of chlorophyll (a, b and total chlorophyll), carotenoids, Baicalin and Pycogenul, and magnetic water by 750 gauss were increase vegetative fresh weight and 500 gauss was increase K% but N%, P%, Fe (µg. g - 1) and protein% not affected by any different intensities to irrigated water.2 - Benzyl adenine at 100 mg. L - 1 concentration increased plant height, leaf area, leaves number, Leaves content of chlorophyll (a, b and total chlorophyll), carotenoids, Baicalin and Pycogenul, while 50 mg. L - 1 of benzyl adenine caused by increased in vegetative fresh and dry weight, N%, P%, Fe (µg. g - 1) and protein% but K% not affected.3 - The use of 3 ml.L - 1 algal fertilizer increased plant height, vegetative dry weight, P%, Fe (µg. g - 1), Leaves content of chlorophyll (a, b and total chlorophyll), carotenoids, Baicalin and Pycogenul. The concentration 1.5 ml.L - 1 algal fertilizer was increased leaves number but not affected in vegetative fresh weight, N%, P%, K% and protein%. The interactions with another factors caused increase of percentages. 4 - The interaction between magnetic water by 1000 gauss with 100 mg. L - 1 benzyl adenine increased plant height, leaf area, leaves number, leaves content of chlorophyll (a and total chlorophyll), carotenoids, Baicalin and Pycogenul. The use of magnetic water by same intensity (1000 gauss) with 50 mg. L - 1 BA increased vegetative dry weight, N%, P% and protein% while b chlorophyll was increase with 500 gauss and 100 mg. L - 1 BA.5 - the interaction between magnetic water and algal fertilizer concentrations revealed that use of 1000 gauss with 3 ml. L - 1 algal fertilizer increased plant height and leaf area, but the same interaction with 1.5 ml. L - 1 algal fertilizer increased leaves number, leaves content of chlorophyll (a and total chlorophyll), carotenoids, Baicalin and Pycogenul.6 - The interaction between benzyl adenine and algal fertilizer showed that used 100 mg.L - 1 BA with 3 ml. L - 1 algal fertilizer increased b chlorophyll, carotenoids and Baicalin, but use of the combination consisting of 100 ml. L - 1 BA with 1.5 ml/L algal fertilizer increased leaves number, leaves content of chlorophyll (a and total chlorophyll) and Pycogenul.7 - The triple interaction between experiment all factors revealed that increased in vegetative dry weight with two combinations (500 gauss magnetic water + 50 mg. L - 1 BA + 3 ml. L - 1 algal fertilizer and 1000 gauss magnetic water + 50 mg. L - 1 BA + 1.5 ml/L algal fertilizer). High P% was combination consisting of (500 gauss magnetic water + 100 mg.L - 1 BA + 1.5 ml.L - 1 algal fertilizer). High total chlorophyll, carotenoids, Baicalin and Pycogenul were combination consisting of (1000 gauss magnetic water + 100 mg. L - 1 BA + 1.5 ml. L - 1 algal fertilizer).
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات - الفسلجة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عبد الامير علي ياسين
اسم المؤلف: سارة عدنان نخاله

دراسة بكتريولوجية كيمياوية وفيزياوية لمياه الشرب المعدنية المتداولة في الاسواق المحلية لمدينة تكريت Bacteriological Chemical And Physical Study for Drinking Mineral Bottled Water Circulating in Local Market in Tikrit City

المستخلص: تضمنت الدراسة الحالية جمع عينات شهرية من اصناف المياه المعدنية المعباة المتداولة في الاسواق المحلية في مدينة تكريت ابتداء من شهر تشرين الاول عام 2010 م ولغاية شهر حزيران عام 2011 م وتم تقييم جودة عشرة اصناف من المياه المعباة بالاضافة الى ماء الصنبور ومقار | The current study included collected monthly samples of mineral water circulating in the local markets in the city of Tikrit from the month of October in 2010 until the month of June in 2011. It was evaluated the quality of ten varieties of bottled water and comparing the results to the standard specifications for Iraqi drinking water and international standards for the World Health Organization.Evaluation included a study of physical, chemical and microbiological factors : turbidity, total dissolved solid, total suspended solid, electrical conductivity, pH, dissolved oxygen, biological oxygen demand, total alkalinity, acidity, total hardness, calcium, sodium, potassium, salinity, chloride, phosphate, nitrate, sulphate, heavy metals, total number of bacteria and total coliforms.The level of quality parameters of these classes were in compliance with the different standards except for pH in one class Pearl and phosphate in one class Rovian.Comparing bottled water to tap water found that the concentrations of turbidity, total dissolved solid, total suspended solid, electrical conductivity, salinity, dissolved oxygen, total hardness, acidity, nitrate, iron, manganese and total number of bacteria in tap water higher than the concentrations in bottled water but didn’t exceed the upper limit that allowed by the local and international specifications for drinking water.Measurements also revealed that the values of most of the criteria listed on the packaging do not reflect the actual content of these water bottles with the exception of one class Kameran most of the packaging in this class has matched the actual content of the water container.The results also showed the absence of coliform bacteria in any of the varieties of bottled water and tap water as well as the significance of the absence of bacterial contamination in it.
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: البيئة
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: حميد سلمان خميس المهداوي ياسين حسين عويد
اسم المؤلف: اسراء حمود عبد الدوري

مستوى الهستامين وبعض المعايير المناعية لمرضى الحساسية التنفسية من النوع الاول The Level of Histamine And Some Immunological Parameters Among Airway Hypersensitivity Type I Patients

المستخلص: تم جمع 50 عينة دم من مرضى الحساسية التنفسية من النوع الاول المراجعين لمركز الحساسية والربو في محافظة بابل للفترة الممتدة ما بين تشرين الثاني 2013 ولغاية شباط 2014, بالاضافة الى 10 عينات دم من الاشخاص غير المصابين بالحساسية من النوع الاول والذين كان معدل I | Blood samples have been drawn from 50 airway hypersensitivity type I patients who visit the center of the allergies and asthma in the province of Babylon, for the period between November 2013 and up to February 2014, in addition to 10 samples from persons with normal IgE level as a control. The sample was divided into two parts, the first used to measure the level of eosinophils and basophils and the second used to measure the level of immune parameters, which included histamine, IgE, IL - 5, IL - 10 and IL - 12 by using the technique of Enzyme - Linked Immunosorbant Assay (ELISA). Study community were divided into three groups, the first group named before immunotherapy treatment and the second group after immunotherapy treatment and final is for control. Conducted differences in the level of immune parameters between the three groups, there were significant differences between the group before the treatment and control group as well as between the group before treatment and after treatment. The level of histamine for the three groups (before treatment and after the treatment and control), reached respectively 34.8292 ± 6.47680 ng / ml and 9.3238 ± 3.55296 ng / ml and 6.9844 ± 3.05477 ng / ml, and the level of IgE reached to the before treatment group 40.6279 ± 15.24231 IU / ml and the after treatment group 23.9529 ± 11.88933 IU / ml and control group 11.7796 ± 5.53 IU / ml, while the cell count of eosinophils for the before treatment group 0.7283 ? 0.24556 x 103 cells / mm 3 and after treatment group 0.3643 ? 0.14861 x 103 cells / mm 3 and control 0.2252 ? 0.09139 x 103 cells / mm 3 , as well as for the cells of basophils for the before treatment group 0.1008 ? 0.03391 x 103 cells / mm 3 and after treatment group 0.0777 ? 0.03485 x 103 cells / mm 3 and control 0.0724 ? 0.03568 x 103 cells / mm 3 , As for the cytokines the level of IL - 5 reached for the same groups 51.3214 ± 6.18565 pg / ml and 22.7254 ± 3.99360 pg / ml and 24.5404 ± 8.64920 pg / ml respectively, while IL - 10 has reached 14.9604 ± 6.48170 pg / ml and 43.0476 ± 21.26668 pg / ml and 24.4969 ± 10.21066 pg / ml, respectively, and finally the level of IL - 12 reached for the same groups 17.753 ± 8.39343 pg / ml and 39.1929 ± 17.73975 pg / ml and 26.7731 ± 12.93092 pg / ml, respectively. There were been a difference in the level of immune parameters for a number of variables, , place of residence, gender, and type of allergy, there were no significant differences observed in the level of immune parameters between these variables. comparison was made in the level of immune parameters for the group after the treatment depending on the duration of immunotherapy, and found that there were significant differences in the level of these parameters with the increase of the duration of treatment. Through this study, conducted the correlation among histamine and the rest of the immune parameters and the results were pointing to the positive correlation of histamine with IgE, IL - 5, eosinophils and basophils and negative correlation with IL - 10 and IL - 12.
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: فريال جميل عبد
اسم المؤلف: سلوى احمد حمزة الكرادي

التشخيص الجزيئي للفطر المحاري Pleuroutus ostreatus وفاعلية بعض المخلفات الصناعية في انتاجه وقابليته الخزنية Molecular Diagnosis of The Oyster Mushroom Pleuroutus Ostreatus And Effectiveness of Some Industrial Waste In Its Production And Its Ability Storage

المستخلص: تضمنت هذه الدراسة انتاج الفطر المحاري ostreatus Pleuroutus باستعمال اوساط زرعية محضرة من مخلفات صناعة محلية وما لها من اهميه من الناحية الاقتصادية كونه يعد عنصرا غذائيا مهما يحل محل البروتين الحيواني المتمثل باللحوم، وما لهذا الفطر من اهمية طبية وعلاجية ا | This study included the production of mushroom oyster Pleuroutus ostreatus by using agricultural media prepared from local industry and which has importance in the economic district it's consider important nutrition element instead of animal protein representive by meat. this fungus has importance of medical and therapeutic, the results showed existence of chemical substances concentrated in the fruiting bodies such as Galic acid and Statin and other substances. that have had an active role in reducing cholesterol in the blood as well as break the clot blood through the presence of certain types of enzymes that break down blood clotting, which may occur, the results showed when studying the mushrooms from the genetic diagnosis molecularly using the technique of the PCR to determine the type of isolation that gives the production of economically significant. Results showed the presence of gene hereditary called Osteareolysin who works with several aspects of the mushrooms which give the recipe contrast pathogenic fungi plant also this study included re - using some of the remnants of plant starch corn husks and Al - gluten and corn embryos. the remnants of Maysan sugar Factory of material Molasses and Molass Baggaz and the study of their role as carrying materials, central ferment and growth of the oyster mushroom during agricultural it on these remnants. The results of the chemical analysis of the remnants of the sugar indestrey in Maysan Molasses and Baggaz and waste plant starch in Al - hashemeyeh / Babylon Al - Baggaz and yellow corn straw showed that molasses substance was contain the amount of sugars which sometimes reach to 41%, that making it as a good base of food to mushroom. When added molasses at different levels 2, 4, 6% to the central media the study showed a positive relationship between the levels of molasses and the radial growth of the oyster mushroom. The concentrations 25, 50, 100% of the leaky innate showed ability Tdhadah high - fungal plant pathogen (Aspergillus niger, Aspergillus flavus, Fusarium solani, Stymphllium sp., Penicillium sp.) Antigonsim when studying the negative ability of the fungus. The results showed a significant increasing in the growth standards and gaining characteristics of the oyster mushroom in the mass production of fruiting bodies called the gaining total according to the wet weight and it was higher production on corn husks and pomace dates 761.89, 735.13 g /kg of media and concentration of molasses 6%. when measuring efficiency of the fungus to product the largest amount of fruiting bodies observed that the middle pea - corn husks and pomace dates gave a larger amount of the remaining media and a concentration of 6% 76.18, 73.51%, have been affected by the weights of pods fungal change is another factor which affected by rates of molasses. The results were closely between nutrition media but nevertheless observed superiority of Corn husks and Pomace dates on the rest of media (15.04, 13.77)by using 6% concentration of molasses. when calculate the lengths of the necks of the body fruiting because of the great importance in a statement recipes - gaining produced and influenced by user medai - aligned and concentrations of molasses, as there were differences moral at all concentrations of molasses, when calculating the percentage of dry matter increased significantly increase the concentration of molasses and all concentrations compared with a comparison of strew wheat, which amounted to 12.16% when adding 2% molasses observed the emergence of highly significant differences between treatments and compared with control treatment, which gave treatment of Pomace dates highest rates, followed by corn husks and corn embryo and Al - gluten and Baggaz 8.21, 9.00, 9.28, 9.29%, respectively. For the purpose of obtaining material efficient in maintaining the objects fruiting intact throughout the storage period tested preservatives (such as Sorbic acid and black pepper) and concentrations 40.20g / l of articles in a row leads to prolong longevity reservoir simulation of the objects fruiting and maintain the quality characteristics of the objects fruiting. The results showed that the addition of material to the feeder molass medai AGRO of oyster mushroom had been increased the proportion of protein in the fruiting bodies, either with respect to calculate the proportion of carbohydrates had been found that the percentage of carbohydrates are associated with a high positive correlation relationship between the concentration of molasses and the different transactions that were used in the experiment. The oils were represented less than what can be had reached attribute to 0%. Well conducted safety checks the health of the mushroom oyster and the results were that the fungus free of toxins by anatomy organic to members liver, kidney and intestines and by the standards of blood physiological mice tested. And the importance of this project calculated the economic viability and profits and revenue obtained from such a project.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: عبد الامير سمير سعدون
اسم المؤلف: سولاف حامد تيموز

تاثير الحمل في بعض وظائف الكبد وعدد من المتغيرات الدموية والكيموحيوية The Effect of Pregnancy on Some Liver Functions And Blood And Biochemical Changes for The Pregnant Women in Al - Alam

المستخلص: تناولت هذه الدراسة تاثير الحمل في وظائف الكبد وعدد من صفات الدم الكيموحيوية حيث تم جمع عينات دم من نساء حوامل خلال مراحل الحمل الثلاث ومن نساء متزوجات غير حوامل كمجموعة سيطرة، وجرت هذه الدراسة في مركز العلم الصحي للفترة من اب 2008 الى ك 1 2009 وبلغ مجموع | This study took the effects of pregnancy on liver functions and number of biochemical blood characteristics, in which blood samples were taken from pregnant women during the three trimesters of pregnancy and from non - pregnant married women as a control group, and this study was done in The Alam health centre from August 2008 to December 2009, and the number of pregnant women were (120) women while the non - pregnant married women were (40) women, there ages were between 15 - 40 years old. The results of this study showed increase in Body Mass Index for pregnant women with progress of pregnancy compared with the non - pregnant married women givingP value (P<0.01) and there was appearance of specific elevation in Fibrinogen concentration in blood and Serum Albumin and Cholesterol concentration and Triglyceride in pregnant women compared with the non - pregnant married women giving P value (P<0.01). Also the results showed specific decrease in Bilirubin during the three trimesters of pregnancy compared with the non - pregnant married women especially in the third trimester giving P value (P<0.01). Also the results showed specific elevation in liver enzymes represented by Alkaline phosphatase and Aspartate transaminase enzyme and Alanine transaminase enzyme especially in the last two trimesters of pregnancy in comparision with the non - pregnant married women giving P value (P<0.01), but for Malondialdehyde, the results showed specific elevation during the three trimesters of pregnancy compared with the non - pregnant married women in contrast to the Glutathione antioxidants that showed specific decrease during the three trimesters of pregnancy in comparasion with the non - pregnant married women giving P value (P<0.01). And the study showed also the effects of pregnancy on the blood elements, in which there was obviouse decrease in number of Platelets during the three trimesters of pregnancy compared with the non - pregnant married women giving P value (P<0.01). And the Heamoglubin concentration, Red Blood Cell number and the mean corpuscle Volume Suffer Specific decrease during the three trimesters of pregnancy compared with the non - pregnant married women giving P value (P<0.01).
الصفحات الاولى:
الكلمات الدلالية:
  • الحيوان - الفسلجة
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2009
الموضوع العام: علوم الحياة
اسم المشرف: صالح محمد رحيم العبيدي
اسم المؤلف: رؤى حسين علوان الجبوري

عزل وتشخيص بعض انواع بكتريا السالمونيلا من بعض الاغذية المحلية في مدينة تكريت Isolation And Identification of Some Species of Salmonella From Some Local Foods in Tikrit City

المستخلص: تم التحري عن بكتريا السالمونيلا في 520 عينة من الاغذية المحلية في مدينة تكريت, وجدت هذه البكتريا في 26 عينة (5%), كانت اعلى نسبة لتواجدها في عينات اللحوم المثرومة (2.5%) والتي تمثل 16.25% من العينات الكلية للحوم المثرومة المدروسة.عزلت السالمونيلا من عي | The presence of Salmonella spp. was investigated in 520 local food samples in Tikrit city, Salmonella was found in 26 samples (5%), with the highest presence in minced meat (2.5%) which was represented 16.25% of all minced meat samples. Salmonella was isolated from chicken's meat at a percentage of 3.75%, and same percentages for meat slices samples, egg cortex and chicken's blood represented 5% for each one, and isolated too from vegetables and cheese at a percentage of 2.5% for each one. No Salmonella has been detected from sweet, yoghurt and egg content. Six Salmonella species were isolated, S. typhimurium was to be the most frequent and represented 42.30% of positive samples, then S. enteritidis and S. anatum in 34.61% and 11.53% respectively, but S. agona, S. typhi and S. arizona existed in a percentage about 3.84% for each one. Also, all the isolated species showed their own serological formula. The selective medium Tetrathionate broth (TTB) was found to be superior for isolation of Salmonella than Selenite cystine broth (SCB), the former positivity was 96.2% and 88.5% respectively. For test of better selective medium for isolation, TTB was found to be more suitable for isolation of S. anatum, while SCB was better for isolation of S. arizona.All the six isolated Salmonella species showed high sensitivity to Ciprofloxacin (5?g) and moderately sensitive to Chloramphenicol (30?g), while they showed high resistance to three antibiotics Amoxicillin (30?g), Nalidixic acid (30?g) and Pipracillin (100?g). They showed resistance with different percentages to the other tested antibiotics : Ampicillin (10?g), Gentamicin (10?g), Streptomycin (10?g), Tetracycline (30?g) and Trimethoprim (25?g).
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: عبد الكريم فتاح عمر
اسم المؤلف: شيماء جابر حميد البياتي

تطبيق بعض طرق حفظ الحشرات على حشرة المن Applicating of Some Methods of Maintainance On Aphids

المستخلص: تعد الحشرات ذات اهمية اقتصادية وطبية وجمالية مما دعت بالباحثين الى دراستها من كافة الجوانب الفسلجية واطوار حياتها وتكيفاتها ومناطق استيطانها وطرق تغذيتها للوصول الى حلول كفيلة للحد من الاضرار المسببة عن الحشرات الاقتصادية والطبية من جانب ولتكثير وتربية ال
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دبلوم عالي
الموضوع الدقيق: علوم الحياة
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: عبد المحسن حسين مؤنس
اسم المؤلف: ضياء عباس عبد الرضا الزيادي

تاثير الماء المعالج مغناطيسيا في بعض الصفات المظهرية والكيموحيوية في عقل نبات الماش (Phaseolus aureus Roxb.) Effect of Magnetically Treated Water On Some Morphological And Biochemical Properties of Mung Bean (Phaseolus Aureus Roxb.) Cuttings

المستخلص: This study was conducted in order to investigate the role of magnetically water in the rooting of fresh and aged (phaseolus aureus roxb.) cuttings, and the impact of treated water in some morphological criteria and biochemical that interfere in the induction of rooting in cuttings and fall clearly when delayed induced auxin treatment in aged cuttings. Study was conducted in the laboratories of the Department of biology at the University of Babylon, for the period 2012 - 2014 was the use of magnetic therapy devices (Magnitron) of water with three gauss 1000, 2000.3000 so as to provide a magnetic flow to distilled water and river water and used as well as the complete randomized design and analyzed the results statistically significant and valuable knowledge LSD to extract the significantly differences between the treatments and the results were as follows : Physiological parts1. Increase the number of roots significantly in cuttings when treat for 24 h.with auxin dissolved with distilled water processor in 1000 gauss amounted to 77.75 root / cutting.2. The results showed that the roots rate increased significantly in the treatment of fresh cuttings treated in distilled water processor 1000 gauss gave 64 root / cutting. As well as the rest treatments affect in rooting increase as given (58, 52.25) root / cutting for each of the treatment 2000.3000 gauss, respectively.3. The treatment of river water as well as magnetically influenced in increasing the number of roots significantly in three processes and the highest being in the 1000 gauss(84) root / cutting.4. The results showed that distilled water processor magnetic effect is significant in the processes that occur during ageing of the cuttings, which led to a reduction in rooting rate in aged cuttings, as it was observed that the treatment of the cuttings for 72 - hour in 1000 gauss is the best of the rest of the treatments amounted to 48 root / cutting. As the impact of river water processor magnetically significantly increase the number of roots in the aged cuttings as treatment given to 1000 gauss higher values 70 root / cuting.Biochemical parts.1. The results showed that chlorophyll content increased significantly when using the river water wizard three intensities (18.09, 17.43, 16.99) (spad) intensities of 1000, 2000.3000 gauss respectively.other each of distilled water and river water processor magnetically in 1000 gauss increase in chlorophyll content to 16.07, 18.57 (spad) respectively in aged cuttings.2. It was observed that the treatment of auxin dissolved in three intensities impacted significantly on the effectiveness of antioxidants defense system and the highest being at the treatment of the cuttings in auxin dissolved water magnetically processor in 1000 gauss, as was the effectiveness of the enzyme SOD 1.85 unit in the effectiveness of the enzyme CAT reached 15.30 unit and increased GSH content to 0.914 mg / g when compared with the control treatment.3. influenced the treatment of fresh cuttings in distilled water magnetically processor with three intensities are equally matched in the effectiveness of SOD and CAT, and the highest being at 1000 gauss magnetic intensity as it affected the effectiveness of the CAT to 31.62 units. Magnetic intensity also affected the same increase in GSH content reached significantly to 0.710 mg / g.4. The results showed the treatment of fresh cuttings river water magnetically wizard in three intensities increase in the effectiveness of the enzyme SOD and CAT, and the highest being at the treatment of 1000 gauss, amounting to 4.168, 45.9 U for both enzymes, respectively, as GSH content increased significantly, reaching 0.810 mg / g in the treatment of the fresh cuttings in 1000 gauss.5. I have not noticed any significant effect of distilled water processor magnetically as well as river water in the MDA content.6. The impact of distilled water processor 1000 gauss significantly in increasing the effectiveness of the enzyme SOD and CAT in aged cuttings, amounting to 2.88, 42.84 units respectively, as GSH content increased to 0.785 mg / g when the treatment of tresh cuttings in distilled water processor with 1000 gauss and also affected the rest of the intensities increase in GSH content in aged cuttings.7. increased the effectiveness of enzyme SOD and CAT when treated of aged cuttings in river water processor magnetically 1000 gauss, amounting to 2.9, 51.2 units respectively, as GSHwas significant increase in content to 0.854 mg / g when treatment aged cuttings in river water processor 1000 gauss and also affected the rest of the intensities increase in GSH content.8. The use of treated water magnetically 1000 gauss significantly effect in reducing the MDA content in aged cuttings 0.293 mg/g fresh weight.
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: بشير عبد الحمزة محمد العلواني
اسم المؤلف: عامر راضي عبد الحسين جبار

انتشار داء المقوسات العيني مع تقييم لبعض جوانب الحالة المناعية في عينة من المرضى العراقيين Prevalence of Ocular Toxoplasmosis And Evaluation of Some Immune Status Aspects In A Sample of Iraqi Patients

المستخلص: اجريت الدراسة الحالية على 104 مريضا من المراجعين لوحدة الشبكية في مستشفى ابن الهيثم التعليمي للعيون في بغداد للمدة من بداية شهر كانون الاول 2013 لغاية شهر حزيران 2014. قسمت مجموعة المرضى استنادا للتشخيص السريري وفحص اختبار التلازن Latex test الى 50 مصابا | The current study was conducted on 104 patients whose underwent to the retina unit in Ibn Al - Haitham Hospital of Eyes in Baghdad from December 2013 to May 2014. The patients groups were divided according to the clinical diagnosis to 50 patients infected with ocular toxoplasmosis (OT) and 54 patents with uveitis, in addition to these groups acontrol group was also included 96 healthy apparent persons without any infection in their eyes.Some epidemiological characters were studied like age, that showed a little effect on two groups OT (Mean of age 38.07 ± 15.14) and uveitis (Mean of age 35.1 ± 17.6). The two groups were found in range 20 - 40 year while the control group include individuals with different age due to the randomly collection. The occupation factor play arole in the prevalence of infection with highly significant differences (P<0.05) in (house wife, workers) with a percentages 35(70%) 35(64.8%) in OT and uveitis groups respectively but the control group expend to include house wife, workers and officers with a total rate 92.7%.The education level also showed significant differences (P<0.01) among the studied groups, the most of ocular toxoplasmosis and uveitis samples were found in highly frequency at the primary, intermediate and secondary educational level with total rate 74% and 77.81% respectively while the control group was found at the secondary and under graduate educational level in 77.1%. The residency character was also examined and no revealed significant difference (P<0.05).The infection path was also set by the presence of animals especially cats which ware existed leading to statistical differences, p? 0.01 for OT group with rate 18%. The visual activity character showed no significant difference (P?0.05) between the patients of OT and uveitis who affected their visual acuity in right eye while the doubling response was recorded for uveitis patients with rate 55.6% in comparable with OT patients with rate 38% for the left eye. The intra ocular pressure was normal in both groups and for both eyes without significant difference.Latex and ELISA IgG. IgM serological tests were used in this study. The results were showed that latex test is easier and it is appear that OT patients were diagnosis with 100%, while the uveitis patients didn't show any positive result. Control group showed that 21 individuals were infected with rate of 21.9%.ELISA - IgG toxo. antibodies confirmed that the infection rate of OT patients was 39 (78%) versus 11(22%) with negative result, while the study also confirm that uveitis patient that have OT disease was 12 patient from 54 case, 42 case didn't diagnosis for OT. The control group was re - evaluated showed 28 case with toxoplasmosis infection from 96 case which from the subclinical group. The antibodies levels were measure by international unit/ ml for all groups, the IgG level was 3.186 ± 1.703 Iu/ml in positive control group followed by OT patients with 2.927 ± 2.417 and uveitis group 0.637 ± 0.803, finally the negative control group 0.276 ± 0.163. IgM levels were low and its presence didn't have any positive case. This study also showed that there was a relation between the IgG level and the pathological effect on eyes which resulted from infection for both groups OT and uveitis, it was high rate 73.5% with 50 patient, for posterior uveitis causing significant differences (P<0.05) followed by anterior uveitis with rate 13.2%.As for the results of cytokines there was a high level of IFN - ? in positive control group 793.7 ± 3461.6 pg/ml followed by OT group 550.6 ± 210.6 pg/ml, the uveitis group that in infected with ocular toxoplasmosis and negative control recorded 524.1 ± 173.6 and 506.6 ± 104.6 pg/ml levels respectively, this rise in the level of IFN - ? cause a significant differences at (P<0.05) for OT, uveitis (non infected) and positive control groups. TNF - ? recorded arise of its level in positive control group 34.89 ± 12.29 pg/ml followed by OT group 32.24 ± 9.91 pg/ml then positive uveitis group 23.61 ± 12.91 pg/ml after them negative control 14.62 ± 5.43 pg/ml finally negative uveitis group with 7.76 ± 4.04 pg/ml.High level of 1L - 12 was gotin positive control group in a concentration 827.00 ± 224.25 pg/ml followed by OT group with 811.28 ± 179.40 pg/ml. An increase in 1L - 17A was recorded in positive control group with mean 327.65 ± 77.89 pg/ml while converged concentration in OT group 263.73 ± 47.48 and positive uveitis group 252.57 ± 61.55 pg/ml. The lowest level was in negative control group 421.6 ± 168.14 pg/ml. MCP - 1 showed a rise in its level in positive control group 274.09 ± 92.05 pg/ml and reduce in OT patients 217.68 ± 98.70 pg/ml, while the two groups negative and positive uveitis recorded levels 155.71 ± 99.51 and 132.60 ± 99.02 pg/ml respectively. The lowest level was register in negative control 97.56 ± 79.17 pg/ml.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: اسراء قاسم العبيدي
اسم المؤلف: عبير فاروق ساكن الحكيم

دور بكتريا الزوائف. Pseudomonas spp في التحلل البايلوجي للمركبات الهيدروكاربونية والمقاومة الاحيائية The Role of The Pseudomonas Spp In The Biodegradation of The Hydrocarbon Compounds And Biological Resistance

المستخلص: شملت الدراسة جمع 60 عينة من مصادر بيئية مختلفة ضمت ترب ملوثة وغير ملوثة بالمركبات الهايدروكاربونية ومياه بزل في محافظة الديوانية ومن مصادر سريرية لمرضى اخماج المسالك البولية في مستشفى الديوانية التعليمي, للمدة من 28 كانون الثاني2013 حتى 1 شباط 2014. هدفت | This study included collection of 60 samples from different environmental sources involved contaminated and non contaminated soils by hydrocarbon compounds and from drainage waters in Diwaniyah province and clinical sources in Diwaniyah teaching hospital, for the period from 28 January 2013 to 1 February 2014. This study aimed to isolation and identification the species of Pseudomonas and metabolic efficiency test in the analysis of petroleum hydrocarbon compounds and measure the effectiveness of antagonism in resistant pathogenic fungi to tomato plant. Thirty four isolates were identified, the Pseudomonas spp. was the highest frequency with 22 isolates. These isolates distributed to 16 isolates of pseudomonas aeruginosa and 6 isolates of pseudomonas fluorescens. Also this study included isolation of two pathogenic fungi which were Alternaria alternate and Fusarium solani in tomato plant. Statistical analysis revealed significant differences at (p<0.05) between the bacterial isolates in their analysis of hydrocarbon compounds, when the optical density measurement as an indicator of the effectiveness of growth on hydrocarbon sources. All isolates showed different optical densities were highest isolation of pseudomonas aeruginosa isolated from urinary tract infections as optical density amounting to 0.23nm. Also showed an increase in electrical conductivity values for all isolates within the incubation period, the highest value to the hospital (1) isolate with 12.8 mc/cm in the third week of the incubation, But decreased in the sixth week to 8.7 mc/cm and all isolates showed a good effectiveness of electrical conductivity in the third week and noted significant differences (p<0.05) in electrical conductivity values for isolates compared with control of 7.2 mc/cm. The results of the DNA electrophoresis of the all isolates contain the two genes (C23O and rhIB) which they code for the production of hydrocarbon - degrading enzymes. In Bio - antagonism experiments noted that all isolates were inhibitory to the growth of two pathogenic fungi and stimulating the growth of tomato plants in glass and field experiments through increase germination, vegetative ratio and root length and increased weights with significant difference at (p<0.05)with seed treatment with fungus only. The best isolates increased in germination were pseudomonas aeruginosa hospital isolate and pseudomonas fluorescens generating isolate which had stimulated germination rate to 76% with significant difference when compared with the control of Alternaria alternata and Fusarium solani which germination rate was 36 and 40 respectively. Pseudomonas aeruginosa which isolated from soil was more efficient in increasing the length of the stem, the stem height was 7.53 cm compared with the control treatment with F. solani, the stem height was 5.7 cm. Pseudomonas aeruginosa hospital which led to an increase in the length of the total root to 3.66 cm compared with the control treatment with F.solani at 1.53 cm. The increase in fresh and dry weights of Total vegetative reached 0.64 gm and 0.065 gm respectively when the treatment of seed contaminated with F.solani fungus and pseudomonas aeruginosa hospital isolate compared to the control which the weights were 0.11 g and 0.011 gm respectively. The highest increase in fresh and dry weights of total root of 0.057 gm and 0.0072 gm respectively when treated seeds contaminated with the F. solani with P. fluorescens hydrocarbon contaminated soil isolate compared with control that the fresh and dry weights was 0.032 g and 0.0017 g respectively. The highest value of stem length registered when treating seed with A. alternata which was 7.5 cm when treating seed contaminated with A. alternata by P. fluorescens isolated from soil contaminated with hydrocarbon compounds. The lowest value of stem length was 7.3 cm for the treatment of seeds contaminated with the A. alternata by P. aerugionsa isolated from the drainage water compared with control treatment (seeds contaminated with A. alternata) which stem length was 6.33cm. As for the fresh and dry weight for total vegetation was the highest value of 0.8 gm and 0.793 gm respectively when the treatment of seeds contaminated with the A. alternata P. aerugionsa isolated from urinary tract infections and the lowest value of fresh weight was 0.61gm for total vegetation when treated seeds contaminated with the A. alternata by P. aerugionsa isolated from soil non contaminated with hydrocarbon compounds and 0.069 gm when treated seeds contaminated with the A. alternata by P. aerugionsa which Isolated from drainage water compared with control treatment which were 0.15 gm and 0.0014 gm of fresh and dry weight for total vegetation, respectively. As for fresh and dry weight of total root were the highest value 0.071 gm and 0.008 gm respectively, when the treatment of seeds contaminated with the A. alternata by P. fluorescens isolated from soil contaminated with hydrocarbon compounds. while the lowest value were 0.058 gm and 0.0065 gm when treated seeds contaminated with the A. alternata by P. aerugionsa isolated from soil non contaminated with hydrocarbon compounds compared to control treatment (seeds contaminated with the A. alternata only)which the fresh and dry weight values for total root were 0.046 gm and 0.0018 gm respectively. When antagonism study in the glass field, the highest percentage of inhibition of the growth of the F. solani on PDA and cetramide agar were 42% and 61% respectively, when treated with P. fluorescens generator isolate. While the bacterial antagonism with A. alternata on PDA media, the highest percentage of inhibition 51% when treated with the transaction P. fluorescens generator isolate on PDA media. Generally, the treatment of pathogenic fungi with biological control bacteria had reduced the percentage of infection and increase the percentage of germination.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: علي عبد رحيم الناشي
اسم المؤلف: عقيل شنان عمران الميالي

الكشف عن السمية الوراثية لمستخلص نبات الصبار Aloe vera باستعمال المؤشرات الجزيئية والخلوية Genotoxic Effect of Aloe Vera Extract Using Molecular And Cytogenetic Markers

المستخلص: نبات الصبار Aloe vera L. من النباتات المهمة والتي تستعمل حاليا بشكل واسع في مجال صناعة الادوية ويضاف هلام الصبار الى اغلب مستحضرات التجميل مع بعض المواد الغذائية في جميع انحاء العالم, لذا تم دراسة التاثيرات السمية الخلوية والوراثية لتراكيز مختلفة من المس | Aloe vera L. is one of the important plants in the world.It is currently and widely used in pharmaceutical industry, and its gel in some time added to some foods all over the world. Therefore, the present study was conducted to evaluate the cytotoxic and genetic effects of crude, alcohol and aqueous extracts of Aloe vera gel toward the onion(Allium cepa L.) roots (as a biological system) exposure at different periods. Onions roots were treated with different concentrations of the extracts : 2% , 5%, 10%, 20% and 40% (v/v)of the crud extract , 5%, 10%, 20% 30% and 50% (v/w) of alcohol extract and the concentration 25%, 50%, 100%, 150% and 200%(v/w) of aqueous extract of Aloe vera gel exposed for different period (24, 48 and 72) hour. The investigation were focused on the effect of these extracts on onions roots length rate and some cellular properties (mitotic index, phase index, the percentage and type of chromosome aberrations, as well as a genetic study using Random Amplified Polymorphic DNA (RAPD) of onions roots treated with different concentration of this extracts. Results showed that all Aloe vera gel extracts had inhibit activity toward onion roots length rate. The inhibition associated positively with the increasing concentration. The effective concentration (EC50%) were 10% of the crude extract, 20% of the alcoholic extract and 100% of aqueous extract, indicating the effectiveness of the crude extract followed by alcoholic extract and then the aqueous extract. Cytological study showed that all Aloe vera gel extract revealed a significant decrease in mitotic index (MI%) of the onion roots cells compared with the control group, and the effect was positively associated with the increasing concentrations of the extract. However, the effect was independent on the exposure period. The concentrations 10%, 20% and 150% of the crude, alcohol and aqueous extract respectively indicated a reduction of the mitotic index to almost 50% of the control treatment. Therefore, this concentrations considered as sub - lethal concentrations for this extracts respectively while the higher concentrations than 30%, 50% and 200% of the crude, alcohol and aqueous extract which reduced the mitotic index to almost 22% of the control treatment were considered as lethal concentrations. The results also showed a significant decreases in prophase and an increase in metaphase in onion roots cells treated with extracts comparison with control treatment. Many of chromosomal abnormalities were appeared due to the treatments of the onion roots with different concentration of the extracts, the percent abnormalities was increased with the increasing concentration of the extract and exposure period. The most frequent of chromosome abnormalities were chromosomal stickiness, disturbed chromosome, chromosomal bridges, C - mitosis, vagrant chromosomes in addition to the other chromosomal abnormalities star telophase. The toxicity effect of Aloe vera gel were studded on molecular level by using random amplification polymorphism of DNA(RAPD). Ten random primers were used, seven of them gave polymorphic bands in all the studied samples and their molecular weights were ranging from 100 to 1600 base pair. The results of Random Amplified Polymorphic DNA (RAPD) showed significant difference in the number of DNA band and their molecular weight of the onion roots treated with Aloe vera extracts compare to control treatment. The high concentrations(higher than EC50%) of extracts were showed to be more effective on the DNA through the appearance or disappearance of larger number of DNA band in comparison with control. The genetic template stability (% GTS) was significantly decreased in DNA of onions root treated with high concentrations (higher than EC50%) of Aloe vera extracts, therefore the high concentrations of Aloe Vera extracts were considered to be genetically toxic. The genetic dendrogram was obtained using Jaccard factor of genetic divergence and the result showed segregation of the samples treated with heights concentrations (%40 200% and 50%) of crude, alcohol and aqueous extract from other samples and control treatment. Therefore, the RAPD method can be considered as an efficient method in detecting for assessment of genotoxicity of crude, alcohol and aqueous extract of Aloe vera gel.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: النبات - الوراثة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: نضال نعمة حسين
اسم المؤلف: غيث منذر فاضل

مقارنة لتشخيص الخمج بالمقوسات الكوندية بين النساء المجهضات والولودات طبيعيا مع دراسة للتغيرات النسجية المتسببة عن الخمج Comparative Diagnosis of Toxoplasma Gondii Infection Among Aborted & Normally Delivered Women With Study of Histological Changes As A Result of Infection

المستخلص: اجريت هذه الدراسة التي امتدت من بداية شهر ايار (2007) لغاية نهاية شهر اب (2008) للتحري عن نسبة الخمج بالطفيلي المسبب لداء المقوسات بين النساء المجهضات والولودات طبيعا في بعض مناطق مدينة الموصل والمراجعات لمستشفى الخنساء التعليمي للولادة والاطفال.شملت عي | This study was conducted during the period from May 2007 to August 2008 to investigate the rate of infection with toxoplasmosis among aborted and normally delivered women in Mosul City territories who attending AL - Khansa'a educating hospital.Out of 150 women under study, 50 were aborted while 100 were normally delivered. The employed tests were included latex agglutination test LATEX and Enzyme Linked Immunosorbent Assay (ELISA) with its two types (ELISA - IgM), (ELISA - IgG) which used as a high specific and sensitive test to differentiate between types of infection acute, chronic and congenital. Toxoplasmosis was diagnosed in 28(56%) out of 50 abortive placenta and in 57(57%) out of 100 non abortive placenta (normally delivered women), these samples of placenta were microscopically examined after homogenizing and staining by Gimsa and Leishman's stain The parasite development stages were also noticed including developing from the tachyzoites into the oocysts stage and the histopathological effects resulting from infection in the placenta in both aborted and normally delivered women which were represented by necrosis, vaculation and cells degeneration , inflammatory cells infiltration and the focal inflammatory reaction in the villi in addition to hemorrhage and accumulation of eosinophilic substances, the results also showed that 41 (82%) of aborted placenta and 18 (18%) of non abortive placenta (normal delivered women) were infected with Toxoplasmosis by using the laboratory Balb/c mice inoculation method while, some cases of inflammation were diagnosed in the brain, of the experimental infected mice, which were represented by the inflammatory cells infiltration and the prevailing of gliosis, tissue necrosis vaculation and cells degeneration.This study revealed that laboratory mice inoculation in the peritoneal cavity is a useful and efficient method in the detection of the parasite in infected placenta as compared with the microscopic examination of direct smears and examination of tissue sections from placenta.The present study showed an overall percentage of infection among aborted women was (92%) while (58%) showed an acute infection and (34%) chronic infection, in another hand the infection rate among normally delivered women was (70%), and it has been found the infection rate among acute and chronic cases were (41%), (20%) respectively, Its to be noted this is the first time in Mosul City for such a study conducted in this area concerning the congenital Toxoplasmosis in newborns with an infection rate (9%).In this study, sera were tested by Latex (LAT), and Enzyme Linked Immunosorbent Assay (ELISA). It was shown that IgG - ELISA is the most sensitive to diagnose the infection as compared to other tests.These study also concerned with the isolation and diagnosis of Toxoplasma gondii parasite from blood by inoculating the blood samples in laboratory Balb/C mice the rate of infection was (16%) in laboratory mice inoculated with blood collected from aborted women while the infection rate in laboratory mice inoculated by blood samples (mother and her neonatal cord blood) collected from normally delivered women appear in (12%) & (16%) respectively. The rate of occurrence of the specific antibodies against Toxoplasma gondii was identified, The highest rate was found for the type (IgG+ - IgM+), in both aborted and normally delivered women with percentage reach (40%) & (26%). The titer (100 IU/ml) of the specific IgM & IgG antibodies was found in high rate reached (75%), (43.5%) in mother blood, also found in a high percentage in the neonatal cord blood estimated as (50%), (64.4%) for the specific IgM & IgG antibodies respectively, in another hand the titer (100 IU/ml) was also found to be the more predominate titer in blood samples of aborted women with percentage reached to (50%) & (60.5%) for the specific IgM & IgG antibodies respectively.
الصفحات الاولى:
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: الاحياء المجهرية
السنة: 2009
الموضوع العام: علوم الحياة
اسم المشرف: ابراهيم شعبان داوود صباح سعيد محمود
اسم المؤلف: فراس محمد بشير عبد الكريم الخشاب

تاثير المعالجة المغناطيسية لمياه الري والسماد العضوي في نمو وانتاج القرنابيط botrytis var. Brassica oleracea صنف Solid snow Effect of Magnetically Treatment of Irrigation Water And Organic Compost On Growth And Cauliflower Production Botrytis Cv. Solid Snow Var. Brassica Oleracea

المستخلص: ا جريت هذه التجربة في قسم علوم الحياة / كلية العلوم / جامعة الكوفة في محافظة النجف الاشرف وتضمنت مرحلتين : الاولى زراعة البذور لغرض قياس نسبة الانبات للمدة من 1 / 8 لغاية 8 / 8 / 2011 وانتاج الشتلات للمدة من 15/8/2011 ولغاية 14 / 9 /2011 في حقل خاص لانتا | This experiment was conducted in the Department of Biology, College of Sciences, University of Kufa in Al - Najaf Al - Ashraf province. It included two periods : the first was seed sowing to measure germination percentage from 1 - 8 till 8 - 8 - 2011, and transplants production from 15 - 8 - 2011 till 14 - 9 - 2011 in a private farm for vegetable transplants production. The second was transplanting in an open field belongs to Plant Production Department/Kufa Technical Institute from 15 - 9 - 2011 till 17 - 1 - 2012 to measure plant growth parameters and curd cauliflower production cv.solid snow in harmless environment techniques. The experiment was adopted as factional experiment. The treatments were distributed according to Randomized Complete Block Design (RCBD) with four replicates. The experiment included the study of two factors : the first, was five magnetic intensities for treating waters magnetically (0; 500; 750; 1500 and 2500 Gauss) by means of dipolar magnetrons. The second was three quantities of plant organic manure(rice residues).i.e.(0, 2.5 and 5.0 kg/furrow) with a length of 6.5 m. and their interactions on growth and production the yield of cauliflower, cv.solid snow. Duncan's Multiple Range Test (DMRT) was used to compare means at probability of0.05. Correlation and regression coefficient were also used to evaluate the relationships between studied parameters and time. Vegetative growth parameters that are measured were : plant leaf number ; stem height; stem diameter; plant leaf area; leaf fresh and dry weight; stem dry weight; plant shoot fresh and dry weight. Flowering parameters were : curd fresh and dry weight and curd diameter. The results could be summarized as following : 1. Water chemical analysis showed that irrigation with magnetized treated water had an effect on physical properties, nevertheless, was more or less affected ions components. 2. The use of magnetized treated water in irrigation resulted in an increase in germination percentage of cauliflower seeds cv. Solid snow.The highest germination percentage was obtained from magnetic intensity of 1500 Gauss in both experiments, laboratory and field that reached 71.14 and 65, 62%, respectively on the base of Arc Sin. 3. The use of magnetized treated water in plant irrigation at all the used intensities resulted in an early emerged curd cauliflower by 15 days compared with control treatment plants. 4. 750 gauss magnetized treated water achieved at age of 138 days from transplanting, the highest plant leaf area mean is (4836.2 cm2) with percentage increase of 51.7% compared to control treatment plant leaf area (3188.3 cm2).That effect was reflected on curd weight. 5. 1500 and 2500 Gauss magnetized treated water treatments at earlier ages increased the values of vegetative growth parameters which reflected its effect on flowering growth parameters by shorter time of curd irritation, compared with the effect of 500 - 750 Gauss at earlier ages , which are in spite of its late effects produced at 150 days old from transplanting particularly 750 Gauss the highest mean of curd fresh weight. 6. 750 Gauss magnetized treated water achieved the highest yield reached 22.2 ton/h. which differed from that productivity gained from control treatment plant(9 - 29 ton/h.) with percentage increase of 138.8%. 7. Organic plant manure addition at both quantities(2.5 and 5.0kg/furrow) resulted in an increase in both vegetative and flowering growth parameters compared with untreated plants. 5 kg/furrow treatment produced the highest means compared to control treatment particularly at the age of 138 days in all vegetative growth parameters. 8. The addition of 5 kg/furrow at age of 150 days produced the largest curd fresh weight 918.4 g. and diameter of 17.2 cm which statistically differed to compared plant treatment that produced 506.0 g. and 13.3 cm. for curd fresh weight and diameter at the same age respectively. 9. 5 kg/furrow organic manure addition resulted in a highest productivity of 21.6 ton/h. compared to that of control treatment of 11.9 ton/h. with an increase of 81.5%. 10. Interaction between irrigation water magnetically treated with 750 Gauss and addition of (organic manure with 5 kg/furrow) at the age of150 days resulted in the highest curd fresh weight of 1292.9 g. with curd diameter of 20.3 cm. compared to that plant of control treatment produced curd fresh weight of 292.5 g. and 11.0 cm. diameter. This interaction also produced 30.3 ton/h. for (750 Gauss×5 kg/furrow) compared with the productivity of control treatment plants that gave 6.86 ton/h. with percentage increase of 341.6%
الصفحات الاولى:
مكان الجامعة: النجف
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: النبات - الفسلجة
السنة: 2012
الموضوع العام: علوم الحياة
اسم المشرف: ثامر خضير مرزة
اسم المؤلف: كوثر عبد الجبار عبد

دراسة بكتريولوجية لبعض الاجناس البكتيرية السالبة لصبغة غرام المقاومة لمضادات البيتالاكتام والمعزولة من اخماج المجاري البولية في محافظة ديالى Bacteriological Study of Some Genus For Bacterial Gram - Negative Resistant To ? - Lactam, Isolated From Urinary Tract Infections In Diyala Province

المستخلص: شملت الدراسة 300عينة جمعت من مرضى مصابين باخماج المجاري البولية وتم جمع العينات في مدينة بعقوبة من مستشفى البتول للولادة والاطفال ومستشفى بعقوبة التعليمي، للمدة بين 1/9/2013ولغاية1/1/2014.اظهرت نتائج الزرع البكتيري على اوساط اكار الماكونكي واكار الدم ووس | Three hundred urine from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al - Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results reved that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli, 22(33.33%) Proteus mirabilis, 9(6.06%)Klebsiella pneumonia, 5(7.57%) Enterobacter spp for 4 (25%) Enterobacter cloacae and 1(5.51%) Enterobacter aerogenes and5(7.57%) Pseudomonas aeruginosa by using diagnostic phenotypic, biochemical tests and confirm the diagnosis using regular aPI20E. Investigation of some virulence factors showed that both Escherichia coli and Proteus mirabilis produced haemolysin 52% and 90.9% respectively. The production of biofilm by local isolates was detected in Congo - red way, Isolates of Escherichia coli and Proteus mirabilis had been shown the ability to produce biofilm 92% and 90.9% respectively. The results showed that all isolates of Proteus mirabilis produced urease by 100%, while the results detect that the isolates of Escherichia coli not able to produce this enzyme. Proteus mirabilis showed ability to produce swarming with 100%. Siderophore production by Escherichia coli and Proteus mirabilis was 48%, 9% respectively. Eschrichia coli, and Proteus mirabilis produced bacteriocin with percentag of 32% and 50% respectively.The production of ? - lactamase by Eschrichia coli and Proteus mirabilis was 60% , 40.9% respectively, also the isolated had the ability to produce the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, The production from each ofEschrichia coli and Proteus mirabilis 12%, 31.8 % respectively. Results of Metallo? - Lactamase by using the Imp - EDTA combination indicated that E.coli and P.mirabilis were 12% and 13.6% respectively. Isolates of E.coli showed highest resistance rate 92% for Augmentin while isolates of P.mirabilis showe higher resistant to cefotaxime 81.8%. The results showed that multiple resistance pattern for antibiotic 43 (91.5%) resistant to (2 - 5) antibiotics, Isolates of E.coli showed highest multiple resistance rate 92% while isolates of P.mirabilis rate 90.9%. The Minimal inhibitory concentration (MIC) for namely two antibiotics Cefotaxime, Ceftazidime were determined.MIC values of these antibiotics ranged between (2 - <1024), (4 - <1024) µg\ml E.coli and (2 - <1024), (8 - <1024) µg\ml P.mirabilis respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique, (9) samples from (10) total, divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: عباس عبود فرحان الدليمي
اسم المؤلف: محمد خضير عباس النعيمي

دراسة وراثية وبكتريولوجية على بكتريا تثبيت النيتروجين التكافلية Ensifer meliloti Bacteriological And Genetic Study On Symbiotic Nitrogen Fixing Bacteria Ensifer Meliloti

المستخلص: عزلت عشر عزلات من بكتريا Ensifer meliloti من العقد الجذرية لنبات الجت Medicago sativa L. بعد ان جمعت من مناطق مختلفة من محافظة بابل خلال المدة من شهر تشرين الثاني/2012 لغاية شهر شباط/2013. شخصت العزلات جزيئيا بتقنية تفاعل البلمرة السلسلي فضلا عن طرق ال | Ten isolates of Ensifer meliloti were isolated from root nodules of Medicago sativa L. collected from different areas of Babylon province during a period from November - 2012 to February - 2013. The isolates were molecular identified using polymerase chain reaction technique, in addition to traditional methods (morphological and biochemical). All isolates were harboured nodbox and mucR genes and produced cyclic ? - (1, 2) glucans, cellulose fibers and lipopolysaccharide.The ability of isolates to grow at different degrees of pH, temperatures and different concentrations of sodium chloride were tested. It was found that the optimum pH for growing of Em1, Em3, Em4, Em6 and Em8 isolates was pH7, while for the isolates Em2, Em5, Em7, Em9 and Em10 was pH8. The optimum temperature for growing of Em1, Em3, Em5, Em7, Em8 and Em9 isolates was 30?C, while the optimal growth of Em2, Em 4 and Em6 isolates was 35?C, Em10 isolate showed significantly extremism to grow at temperture reached to 45?C. The optimal percentages of NaCl for growing of Em1, Em3, Em4, Em5, Em6, Em8 and Em9 isolates were 2%, for Em7 isolate was 3% and for Em2 and Em10 isolates were 4%. The effect of different degrees of pH on symbiotic properties was studied. Em1 and Em8 isolates that grew at pH6.5 ; all isolates that grew at pH 7, pH 7.5 and pH 8 were able to infect the root hairs of alfalfa plants. The isolates that grew at pH6.5 (except Em1 and Em8 isolates) and all isolates that grew at pH8.5 were failed to infect the root hairs and did not formed nodules and resembled to the control plants (without inoculum).Different concentrations of NaCl on symbiosis was studied, it was found that all isolates that grew at 2% and Em2 and Em10 isolates that grew at 3% formed nodule, while Em1, Em3, Em4, Em5, Em6, Em7, Em8 and Em9 isolates that grew at 3% and all isolates that grew at 4%, 5% and 6% did not elicited nodules on the roots of alfalfa plant. Light microscopic observations of root hairs inoculated with Ensifer meliloti isolates showed the root hair deformations like curling, waviness, swelling and formation of shepherd`s crook and infection thread. These consequent changes lead to emerge the mature nodules having five distinct zones viz., distal meristematic, infection, inter, nitrogen - fixing and proximal senescence zones.The gel electrophoresis results of plasmid DNA of isolates showed that these isolates harboured two megaplasmids. When the isolates exposed to extreme factors like pH, temperture and NaCl, it was found that the isolates lost these plasmids and its ability to elicite the nodules and failed to fix the nitrogen. This study showed that the nitrogen fixing bacteria had essential role to support the plant growth and increase their productivity, and recommended to use the isolate Em10 as example for biofertilizers and the best alternative to the chemical fertilizers
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: حسن فاضل ناجي
اسم المؤلف: محمد زهير ناجي المرعب

دراسة انتشار انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا Studying The Spreading of Fungus Saprolegnia SPP. In Waters of Diwaniyah City And Characterization The Molecularly

المستخلص: شملت الدراسة الحالية عزل وتشخيص بعض الفطريات المائية ومنها انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا. عزلت الانواع التابعة لهذا الفطر وهما Saprolegnia parasitica وSaprolegnia ferax من الانهار والبحيرات والاسماك, وتم قياس الخصائ | The present study is concerned with isolation and identification of some aquatic fungi, including species of Saprolegnia spp. in waters of Al - Diwaniyah city and molecular characterization. Two species, Saprolegnia parasitica and Saprolegnia ferax, have been isolated from rivers, lakes and fishes. The physical and chemical environmental characteristics have been measured. The types above have been examined to extract the DNA. The (PCR) technique has been used to examine the presence of protease enzyme. The results of isolation and identification showed that the percentage of the two species were 64% in rivers areas 40% in the lakes areas and 50% in fishes. The rates represented the examined samples which were 60 samples. AS well as isolates was in rivers 16 isolation and in Lakes 10 isolates either in fish was 5 isolation and thus the total isolates 31 isolation, The S. parasitica species is more apparently found during (February, 2014), the number of samples was 12, the less presence of this species in (April, 2014), where the number of sample, was 3. The S. ferax species is more apparently presented in (February, 2014), the number of samples was 10. The less presence was in (April, 2014), the number of samples was 2. The results showed that the number of isolation of Saprolegnia spp. study area as it was S.parasitica species in were 6 in rivers, 2 in lakes, and 3 in fishes. The second species, S. ferax showed number of isolations which were 10 in rivers, 8 in lakes, and 2 in fishes. The PCR examination has used to detect the rDNA gene which responsible for proteases gene and whether it is found in the two species of fungi. The express way has been used to extract DNA and according to the instructions of manufacturer company. The concentrations of DNA in first species were 121.8 - 73.0 mcg/microliter and purity 1.87 - 1.80.In the second species the concentrations of DNA were 2360.4 - 166.9 mcg/microliter and purity 2 - 1.75. The results, by using PCR technique, showed that the percentage of protease in first species was 25% in rivers. In lakes and fish was 100%.Concerning the second species, the percentage of enzyme was 42% in rivers, 40%in lakes, and 50% in fishes. It can be concluded that there is a diversity in fungi species with respect to study areas and enzyme secretion in the stages of fungi infection. The enzyme is more apparently found in S.parasitica species compared with S.ferax species.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: ماجد كاظم عبود الشبلي
اسم المؤلف: معتز محمد عزوز الزاملي

دراسة مصلية دمية لداء المقوسات في النساء الحوامل والاطفال حديثي الولادة في محافظة القادسية Bloody Serological Study of Toxoplasmosis In Pregnant Women And Newborns In Diwaniyah Province

المستخلص: اجريت الدراسة الحالية على الاطفال حديثي الولادة في محافظة الديوانية للمدة من بداية شهر تشرين الثاني 2012 الى نهاية شهر نيسان 2013.وقد جمعت عينات الدراسة من دم الحبل السري للاطفال الرضع المولودين حديثا اثناء الولادة في مستشفى الاطفال والولادة التعليمي في | The present study was done on new born babies in Diwania province during a period from the beginning of November 2012 till the end of April 2013. Blood samples were collected from the umbilical cord of the new born babies during delivery in children and maternity teaching hospital. Fifty samples were collected separated to serological tests and for blood pictures. Serological tests were used in this research as follows (Rapid IgG - IgM test, latex test and ELISA IgG, IgM). The results showed the Rapid test was the easiest and cheapest test for diagnosis specific antibodies against Toxoplasmosis, therefore the positive samples were 14% for IgG, 4% for IgM and 36% for overlap cases. While the positive results for latex test was 14% but the highest result was recorded by using ELISA test 30% for IgG, No positive result recorded for IgM. There were variation in positive cases of different ages of mothers, the highest percents found in ages between 30 - 39 years old by using Rapid test 72.72% , while by using The latex test demonstrate high percent of infection was 20% and also for ages between 15 - 19 year old, and the ELISA recorded 50% and the same group of age 15 - 19 years old. The results showed the for the residence factor by using Rapid test showed the rural people were high percent of incidence of Toxoplasmosis 57.14%, but the urban people recorded Latex test and ELISA)17.24%, 31.03%) respectively. Aborted women in previous time were followed by using the serological tests Rapid test 10%, Latex 10% and ELISA zero. The incidence of Toxoplasmosis was high in housewives more than other career, the results were) 34.14%, 34.14 (% for Latex and ELISA, IgM and overlapping cases, while in other career by using Rapid test 66.66% Results showed standard Blood parameters did not register significant differences (P> 0.05) for the total number of white blood cells WBC, hemoglobin Hb, blood volume compact PCV, Erythrocytic RBC, and differential count of white blood cells for Neutrophiles, Lymphocyte, Monocytes, Basophiles and Eosinophiles. Phagocytic activity was prominent in adult people more than newly born babies.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: خيري عبد الله داود العكيلي
اسم المؤلف: مروة جبار مزهر العبادي

تاثير المجال الكهرومغناطيسي في بعض صفات النمو والحاصل والانقسام الخلوي لنبات العصفر Carthamus tinctorius L The Effect of The Electromagnetic Field On Some Growth Characteristics And Yield And Mitotic Index For Carthamus Tinctorius L

المستخلص: اولا : - التجربة الحقلية : اجريت التجربة في الحقل التابع لقسم علوم الحياة - كلية التربية للعلوم الصرفة - ابن الهيثم، وصممت باستخدام تصميم القطاعات الكاملة المعشاة Randomized Complete Blocks Design (RCBD) حيث تم تعريض البذور قبل الزراعة لمجال كهرومغناطيسي | First : - Field experience : the experiment was conducted in the Department field of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using design Full sectors Randomized Complete Blocks Design (RCBD) where the seeds exposing to electromagnetic field in the Department of Physics - Electrical Laboratory - College of Education, Pure Science - Ibn al - Haitham before planting them in five intensities (0, 5, 10, 15, 20) mT and two (1.2 and 3) an hour for each intensity to see the electromagnetic field effect in some of the qualities of growth and yield plant Safflower the results were as follows : The effect of electromagnetic field strength was positive traits and was the highest increase in exposure to the intensity of 10 mT for each of the (percentage of germination, root size of the second date, plant height, leaf area, leaf area guide, the dry weight of the root system at the time the second, fresh weight of shoots at the first appointment, the average absolute growth, the sustainability of biomass, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (81.21%, 18.33 cm 3, 93.37 cm, 71.29 cm 2, 1.187, 8.21 g.plant 1, 155.13 g.plant - 1, 1.76 g.yum 1, 691.67 g, 2.03%, 0.733%, 2.47%, 12.96%, 56.96 spad, 44.55 Nora.plant - 1, 46.60 g.plant - 1, 5.35 g, 161.80 kg. acres - 1, 38.74 mg. plant - 1, 12.09%, 13.50%), respectively, except for the size of the root first date, fresh weight of the total root for the first two dates, and second, the dry weight of the root system in the first date, fresh weight of shoots in the second deadline, the dry weight of shoots for the first two dates, and second, the specific weight of the oil had the highest increase at exposing the seeds to the intensity of 15 mT and average (15.16 cm, 15.12 g. Plant - 1, 26.00 g plant - 1, 3.98 g plant - 1, 411.08 g.plant - 1, 20.03 g. Plant - 1, 69.15 g. Plant - 1, 1.069%, respectively) and the highest increase of the proportion of total oil when exposure to the severity of 5mT an average 33.94%. The effect of the length of time in field was significant in most of the qualities which gave the term two highest increase with the exception of germination percentage was the highest increase in exposure for a period of three hours, with an average (60.79%, 91.01 cm, 67.51 cm 2, 1.124, 131.93 g. Plant - 1, 337.61 g. plant - 1, 1.68 g. Day - 1, 1.92%, 12.04 g. plant, 55.99 Spad, 146.06 kg. dunams - 1, 4.77 g, 40.23 mg. plant - 1, 28.03%) for each of the (germination percentage, plant height, leaf area, leaf area guide, fresh weight of shoots in the first date, fresh weight of shoots in the second deadline, the average absolute growth, nitrogen, protein, chlorophyll, yield seeds, 100 - seed weight, carbohydrate content, the total percentage of oil). As was the overlap between field strength and duration significant effect on most of the traits with the superiority of treatment (10 mT for two hours) on the rest of the other transactions for each of the (germination percentage, the size of the root of the first two dates, and the second, plant height, number of branches, leaf area, area guide paper, fresh weight of the root system of the first two dates and II, , dry weight of the root of the first two dates, and the second, fresh weight of shoots for the first two dates, and second, the dry weight of shoots for the first two dates, and second, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, He yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (39.33%, 16.50 cm, 19.50 cm, 103.86%, 17.00, 79.83 cm, 1.330, 16.43 g.plant - 1, 29.20 g. plant - 1, 4.16 g.plant - 1, 10.48 g.plant 1, 184.06 g. plant - 1, 416.33 g. plant - 1, 22.35 g. plant - 1, 73.25 g. plant - 1, 2.10%, 0.800 %, 3.04%, 13.15%, 60.31 spad, 49.14 Nora.plant - 1, 53.37 g. plant - 1, 6.14 g 185.97 kg. acres - 1, - 1.plant 47.81 mg, 2.28%, 14.25%), respectively, with exception each of the average of the absolute growth and sustainability of biomass, where the highest increase in exposure to the intensity of 10 mT for a period of three hours and average (1.85 g.yum 1, 724.85 g.yum), respectively, and total percent of the oil was the highest increase in exposure to the intensity of 5 mT and for three hours and an average of 37.28% and the intensity of 15 mT for a period of three hours, specific gravity of oil and an average of 1.081%Second : - laboratory experiment : experiment was conducted in the laboratory of physiology of the plant Higher Studies - Department of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using the full random design Completely Randomized Design (CRD) to study the electromagnetic field effect five intensities (0, 5, 10, 15, 20) mT and two (1.2 3) per hour on the severity of the cell division of the safflower plant results were as follows : The results showed that there is a positive effect of the electromagnetic field on the traits and was the highest increase at exposing the seeds to the intensity of 10 mT for each of the (number of dividing cells of the tops of developing the parties to the roots, the total number of cells to the tops of the parties to the roots, and guide cell division developing the tops of the parties to the roots) and average (156.00% 1143.00%, 12.17%), respectively. The effect of duration of exposure, and had a significant effect on the average number of total cells to the tops of the parties to the roots reached 1042.20% when the exposure for two hours.The effect of overlap between the severity and duration of the electromagnetic field significant was effect in all cellular qualities and was the highest increase in exposure to the intensity of 10 mT for a period of one hour and average (167.00%, 13.52%) for each of the number of dividing cells developing the tops of the parties to the roots and guide cell division to the tops of developing the parties to the roots, The total number of cells to the tops of the parties to the roots was the highest increase in exposure to the intensity of 10 mT for two hours and an average of 1203.50%.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
الموضوع الدقيق: النبات
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: ماهر زكي فيصل الشمري
اسم المؤلف: مروة قيس ابراهيم العبيدي

تاثير المستخلص الكحولي لنبات الخباز (Malva sylvestris ) في فسلجة ونسجية الكبد والخصية في ذكور الفئران البيض Effect of Alcoholic Extract of Mallow (Malva Sylvestris) On Physiology And Histology Liver And Testis In Albino Male Mice

المستخلص: صممت هذه الدراسة للتعرف على مدى تاثير المستخلص الكحولي الخام لنبات الخباز Malva Sylvestris في الكبد والخصى لذكور الفئران البيض نسجيا, وكذلك فسلجيامن خلال دراسة مستوى انزيمي Glutamate - oxaloactate - transaminase (GOT) وGlutamate - pyruvate - transaminase( | This study was designed to investigate the effects of crude alcoholic extract of Malva sylvestris on the histology of liver and testes in male albino mice, as well as levels of the GOT, GPT enzymes and testosterone hormone. Seventy two adult male albino mice were used in this study. The animals were randomly divided into four main groups, the first group was given distilled water (control), while the other three groups were given (100, 300, 500 mg /kg) of crude extract. These main groups were subdivided into three subgroups, according to the period of treatment (2, 4, 6, 8)weeks. Blood samples were collected every two weeks for physiological study, and samples of the liver and testes were collected for histological study. Treating with crude alcoholic of Malva sylvestris led to no significant increase in the mean of the body weight except the concentration (500 mg /kg). There was significantly increase in the liver weight particularly in(500 mg /kg) for eight weeks of exposure, also there was significantly increase in the average of the testes weights treated with Malva sylvestris extract specially in (300, 500 mg /kg) for all periods of treatment compared with control group. Malva sylvestris extract coused significantly decrease in both GOT, GPT. there fore the value of GOT, GPT considers as concentration - period exposure dependent the highest value of GPT was (300 mg /kg) for two weeks (2. 25±228. 04) IU/L compared with control group (3. 03±226. 02) IU/L. and the lowest value of GPT was (500 mg /kg) for eight weeks (4. 52±192. 27)IU/L compared with control group (4. 06±208. 77) IU/L the highest value of GOT in (100 mg /kg) reached (4. 10±63. 00) IU/L for two weeks compared with control group (1. 25±71. 23) IU/L. the lowest value was (2. 77±49. 83) IU/L in (500 mg /kg) with eight weeks comparison with control group (1. 18±69. 02) IU/L. all concentration of Malva sylvestris extract made positive changes in the value of testosterone with all periods of exposure particularly in (300 mg /kg) compared with control group. There were many histopathological alterations appeared in the liver tissue among treated groups which caused by the effects of Malva sylvestris extract represented by congestion, disarrangement of hepatic cords, beginning of necrosis of hepatocytes, infiltration of leukocytes, narrowing of sinusoids, increase the number of binuclei cells, degeneration, appearance of giant cells, all these changes were reversible. Malva sylvestris extract made beneficial histopathological changes in the testes tissue in all treated groups like increase in numbers of ledyig cells specially concentration (500 mg /kg) reached (11.37± 0.80) IU/L with two weeks comparison with control group(5.38 ±0.34) IU/L, and increase in the thickness of the walls of seminiferous tubules specially concentration (500 mg /kg) reached(29.46± 1.39) IU/L with compared with control group(20.52±2.40) IU/L. In addition the role of extract in significant decline in the diameters of seminiferous tubules in all treated groups compared with control group
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الحيوان
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: مختار خميس محمد سعيد
اسم المؤلف: بسمة علي جاسم

دراسة العلاقة بين فقدان الجينين الـ GSTM1 وGSTT1 والاصابة بمرض الربو القصبي في محافظة ذي قار Study The Relationship Between The Null Genotype of GSTM1, GSTT1 Genes And Bronchial Asthma In Thi - Qar Province

المستخلص: تم تصميم هذه الدراسة للتحقق من دور جينات الـ Glutathione - S - Transferase (Mu - 1) gene و(Theta) - 1 Glutathione - S - Transferase ومدى مساهمتها بخطر الاصابة بمرض الربو القصبي في محافظة ذي قار. حيث شملت (105) شخص مصاب بالربو باعمار من (20 - 69) سنة كان | This study has been designed to investigate the roles of the gens GSTMI and GSTTI the extent to which they contribute in asthma at Thi - Qar governorate. The study has been conducted on (105) patients with asthma whose ages from (20) to (69) years. (20) of them are males and (85) are females, and (110) are healthy with no infection of asthma or any sickness of respiratory system as a control group of the same ages, (60) of these are males and (50) are females. Blood samples have been collected from both groups in ampoules with EDTA and stored under temperature ( - 20) until they are used in getting the DNA from the groups. The genes GSTTI, GSTMI, and Albumin have been enlarged as internal control employing Polymerase chain Reaction (PCR). Some dangerous aspects of this disease have been investigated, the results showed that the disease in the urban areas (85. 71 %) higher than it rural areas, and Asthma among females is (80. 95%) higher than males. The results also indicated that asthma in ages (50 - 59) is more than other categories; it is (34.28%). The majority of patients are smokers with (63.80%). The study revealed that (73.33 %) of the patients have a family history with the disease while (26.66 %) have not. Statistical analysis showed that the loss of the gene GSTM1 is about three times in the patients more than the case in the control group. (OR=2.90) , while the loss of the gene GSTT1 is five fold more than it compared to the control group (OR= 5.55). The results indicated a significant effect of the loss of the two genes in having asthma in the urban and rural areas, it is six times and a half larger in the urban areas compared to the control group of the same area. Asthma probability is ten times more in the case of the loss of both genes in the rural areas compared to the control group in the same area. And no significant difference has been discovered when gene patterns of patients in both areas. The results also indicated that the probability of having asthma in male patients is two times and a half as many as it in the females when the gene GSTM1 is lost compared to the control group. While the same probability is eleven times in the case of the loss of both genes in the females as many as it the control group. The loss of Missing the gene GSTT1 is three times in the male patients and five times in the females compared to the two control groups. The results indicated significant differences when gene patterns of males and females are compared in the case of the loss of both genes. The statistical analysis indicated that the loss of both genes in the smoker patients contributes 17 fold in the probability of having the disease compared to the control group. While the probability increases four times approximately in the nonsmokers when the gene GSTM1 is lost compared to the control group (OR=3.77; 95% C1=1.69 - 8.407). No difference has been noticed in the gene patterns of the smoker and nonsmoker patients. The results indicated that the loss of both genes has been in all the age categories. No significant differences have been noticed among the gene patterns between the patients of less and those who are more than 50 years. Asthma probability has been found 7.77 times more in the case of the loss of both genes in the patients who have a family disease history compared with the control group. The asthma probability is twice when the gene GSTM1 is lost in those patients who do not have a family history disease compared to the control group. Upon comparing the gene patterns of patients according to the family disease history, there has been an effect of the loss of both genes and having a family disease history on the probability of asthma, it is almost six times when both genes are lost. The results indicated that the probability increases three times in the loss of the gene GSTT1 in the educated patients compared to control group. The probability also increases seven times when both genes are lost in the uneducated patients compared to the control group. When comparing the gene patterns of patients according to levels of education, significant differences between the educated an uneducated have been approximately twice in the loss of the gene GSTT1.
الصفحات الاولى:
مكان الجامعة: ذي قار
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: حسن ريسان الركابي عدنان عيسى البدران
اسم المؤلف: نـور عمران عبد الكريم الغـزي

الفعالية المضادة للاحياء المجهرية والانزيمية للفطريات المستنبتة المعزولة من نبات الاس Myrtle (MyrtuscommunisL.) Antimicrobial And Enzymaticactivity of Endophyticfungiisolatedfrommyrtle (Myrtuscommunisl.)

المستخلص: استهدفت الدراسة الحالية عزل وتشخيص الفطريات المستنبتة في اوراق نبات الاس Myrtus communis واختبار فعاليتها المضادة للاحياء المجهرية وفعاليتها الانزيمية. - اظهرت نتائج عزل الفطريات المستنبتة في 500 قطعة اوراق للحصول على عدد كلي للعزلات مساويا الى 99 عزلة ف | The present study was aimed to isolate and identify endophytic fungi from leaves of myrtle Myrtus communisand study their antimicrobial and enzymatic activities. - Results of fungal isolation from 500 leaves fragments revealed isolation of 99 endophytic fungi represented a total colonization frequency (CF) of 20.4%. These fungi were related to 23 species or isolates, where Aspergillus spp. prevailed over all others (11 species) with CF of 14% for all Aspergilli, Aspergillusniger and Aspergillusflavus showed the highest CF 7.2% and 3% respectively, other Aspergilli represented Aspergillusparvulus (CF 1.6%), Aspergilluscandidus (0.4%), Aspergillusraperi, Aspergillusclavatus, Aspergillusglaucus, Aspergillusornatus, Aspergillussclerotioniger, Aspergillusterreus and Aspergilluswentii were all showed CF of 0.2%.Other isolates fungi from leaves fragments were four morphologically different isolates of Penicillium with 0.4% CF for each. Additionaly, isolates of Cladosporium sp. (2%) and Cladosporiumcladosporoides (0.4%), as well as Cunninghamella sp., Drechsleraaustraliensis, Alternariaraphani, which showed 0.2% CF for each, Paecilomyces variotii (0.6|%), and different isolates of sterile fungi (White and Hyaline colonies) were also obtained. - Study the antagonistic activity of isolated endophytes using dual culture method against five plant pathogenic fungi(Fusarium oxysporum, Fusarium graminearum, Fusarium sp., Macrophomina phaseolinaandRhizoctonia solani)taking into account the percentage of inhibition of pathogenic fungi, results revealed variation of antagonistic activities between endophytes and pathogens. Different cases of antagonism were also appeared ranged from mycoparasitism of the endophytes on pathogens or vice versa, to competition between the two fungi and to the appearance of inhibition zone between them, this result may indicates the affinity of using some isolated endophytes in biological control applications against or controling fungal plant diseases. - Effect of ethylacetate extracts of culture broth of active endophytes shown in the last experiment against two bactria E.coli, S.aureus and the yeast Candida albicans using disc diffusion method, where filter paper discs were imprignated with different concentrations of fungal broth extracts. Results showed variation of inhibitory activity against bacteria with different endophytes, bacteria and conc. of the extract. Regarding the effect onC.albicans and filamentous fungi, most fungal broth extracts showed no inhibitory activity against yeast and no extract as well showed inhibitory activity on filamentous fungi. These results give indication thatbroth extracts contain antibacterial compounds. - Addition of 20% crude fungal broth of some endophytes to the culture medium showed significant inhibitory activity against filamentous fungi which did not respond to broth extracts, percentage of inhibition ranged between 0 - 77.08% and maximum inhibitory activity was shown by Penicillium sp. Isolate. These results give indication that some of crude filtrates contain antifungal compounds. - Analysis of broth extracts of some endophytes using TLC technique showed the content of extract of number of UV illuminated spots isolated on TLC pates having different Rf values, Bioautography showed inhibitory activity of some of these isolated spots against E.coli, S.aureus and the Filamentous fungus F.oxysporum. - Testing the extracellular enzymes activities of isolated endophyles on solid media, enzymes included amylase, lipase, pectinase, laccase, cellulase and protease showed variation in secretion of these enzymes, as well as, no endophytes showed the affinity of secreting all tested enzymes. Results also showed the high affinity of some endpohytes to secrete enzymes that could be use in multiple important applications after separation andcharacterizationof these enzymes.
الصفحات الاولى:
مكان الجامعة: بغداد
اللغة: العربية
الجامعة: جامعة بغداد
الدرجة: ماجستير
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: بتول زينل علي
اسم المؤلف: هدى محمد كاظم ديوان

التوصيف المظهري والجزيئي لبعض الفطريات الخيطيه والخمائر المعزولة من اخماج العين واختبار حساسيتها الدوائيه تجاه بعض المضادات الفطرية Morphological And Molecular Identification of Some Filamentous Fungi And Yeast Isolated From Eye Infections And Theirs Susceptibilities Toward Some Antifungals

المستخلص: تمت هذه الدراسة في مختبر التقنيات الاحيائية التابع لكلية علوم البنات / جامعة بابل للفترة من اكتوبر 2012 الى اكتوبر 2013، حيث تم جمع 165 عينة سريرية من المرضى المراجعين لاستشارية العيون في مستشفى الحسين العام ومن بعض العيادات التخصصية لاطباء العيون في محاف | The study was performed in Biotechnical Laboratory in Science collage for women, Babylon University from Oct.2012 to Oct.2013. 165 clinical samples were collected from patients with eyes infections were admitted to AL - Hussein main hospital in Karbala province and private clinics of eye physician, after diagnosis of eye infection by specialize physician the clinical samples collected by using swab from Cornea, Conjunctiva, Lacrimal sac, eye allergic, contact lenses and from pull water eye after surgery, the samples included different sex & age groups.All clinical samples culturing on SDA and PDA, Yeast was identified base on their color reactions on Chromo agar Other biochemical test were performed.Antifungal sensitivity was performed against 8 antifungal (Nystatin, Econazol, Amphotericine B, Flucosytosine, Miconazole Metronidazole, Griseofulvin, Ketoconazol, Most fungi showed variable degrees in their susceptibilities to the antifungal agents. In this study we diagnostic (57) isolate from filamentous fungal species, Aspergillus spp. and Fusarium spp. showed highly frequent in ratio 20.1% and 9.6, In addition (37) yeast isolated, C.albicans and C.famata representative the highest frequent isolates.Molecular identification and genotyping, 8 isolates identified as C.albicans from 24 of selective Candida isolates were performed by specific pair primer (CABC) that give genotype with 515 base pair, genotype ITS1/ITS4 region show polymorphic PCR product help in diagnosis other (7) Candida species like C.famata, C.rugosa, C.glabrat, C.utili, C.saitoana, C.guilliermondii, C.inconpicua. The pair primer for amplified the transposable intron (CA - INT) was succeed in amplification of intron showed two genotypes : type A (450bp) and type B(850bp) and showed there was no C.dubliniensis isolates in our Candida isolate, This result was coincidence typing results between specific pair primer (CABC) and Microsatellite CAI for C.albicans.The amplified 18s region by using NS/EF3 and the result give genotype with molecular weight 1700bp.Also the study include using RFLP technique & the digested by using CfoI enzyme & the results for ITS PCR products of 24 isolates of Candida showed unique RFLP patterns.
الصفحات الاولى:
مكان الجامعة: بابل
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الاحياء المجهرية
السنة: 2014
الموضوع العام: علوم الحياة
اسم المشرف: زيدان خليف عمران المعموري
اسم المؤلف: يسر فاضل عبد الامير الاسدي

تقييم فعالية قاعدتين من قواعد شف الجديدة في بعض انسجة ذكور الفئران المختبرية البيض المصابة وغير المصابة بطفيلي المتحولة الحالة للنسج Entamoeba histolytica Evaluate The Effectiveness of Two Bases of The New Schiff Bases In Some Tissues of Male Laboratory Albino Mice Infected And Non - Infected With The Parasite Entamoeba Histolytica

المستخلص: تضمنت الدراسة الحالية تحضير وتشخيص قاعدتين من قواعد شف، حضر الليكاند الاول L1 من تفاعــل 2 - هيـدروكسي - 1 - نفـثالديهايد مــع الـسلفاميثوكسازول، وحضر الـثانـي L2 مـن تـفاعــل 4 - استميدوبنزالديهايد مع 4, 3 - داي امينو تلوين. شخص الليكاندان باستخدام ت | The present work includes a preparation and characterization of two schiff bases compound, the first from (L1) reaction of 2 - hydroxy - 1 - naphthaldehyde with sulphamethoxazole, and the second from (L2) reaction of 4 - Acetmidobenzaldehyde with 3, 4 - Diamino toluene. These compounds were characterized by the Infrared (IR) Spectroscopy, Nuclear Magnetic Resonance Spectroscopy (1H - NMR), Mass Spectra, and the results were virtually identical to what is expected. It has been determined LD50 for the two ligands - after dissolved dimethyl sulfoxide DMSO - and account for them safe therapeutic dose, reaching 0.14 g / kg and 0.24 g / kg, respectively. In order to see the effectiveness of the two ligands on the parasite compared with the infected group and treatment with a drug metronidazole, these two ligands have been administered orally the therapeutic dose each separately on two groups of mice which infected by the parasite Entamoeba histolytica. Three other groups of mice that non - infected have been also administered separately with the parasite first and second ligands and dimethyl sulfoxide, to see some of the side effects of these three compounds in comparison with the two groups of positive and negative control. At the level of probability (p ? 0.05), the results of statistical analysis show a significant variation, remarkable inhibitory effect of the number of cysts that reached the less impact to the second ligand rate about 2.9. in the seventh day of the injury and treatment. In the fifth day of the injury and treatment, the highest inhibiting effect of metronidazole was at a rate of 0, while in the seventh day of them, the first ligand was a compromise in effect, amounting to at a rate of 1.1. The Histological study of tissue of the cecum, liver and spleen showed a normal state for a negative control, and an occurrence of severe tissue changes in a positive control. In the cecum tissue, the mucosal was thin, and there were crashes in the crypts of Lieberkühn, with infiltration in inflammatory cells, as well as the expansion of the cells lining the crypts, and the disappearance of or crash submucosal layer, as well as severe hemorrhage in muscularis, and thickening in serosa. There was severe congestion of the blood vessel which was observed in liver tissue, as well as thrombus which appeared attached to the wall of the blood vessel, and infiltration of inflammatory cells out of the blood vessel toward the hepatic tissue, especially macrophages and eosinophils. It was also noticed the disappearance of hepatic architecture with degeneration of the liver cells and a clear sinusoids expansion. In addition to vacuolation of liver cells, that the tissue sections showed, with the occurrence of bleeding along the hepatic tissue. The changes in the spleen tissue represented in the occurrence of severe bleeding on the length of the lymphatic tissue of the spleen with infiltration of inflammatory cells, especially macrophages, and the happening of megakaryocytes. There is a remarkable improvement could be noticed in each tissues of cecum, liver and spleen of the infected groups and treatment with metronidazole and the first and second ligands. The improvement ratios were close to some extent : in the treatment group with metronidazole there was observation in tissue cecum to the occurrence of the four layers of its component, and the happening of a little bleeding which, with infiltration of inflammatory cells, and this is roughly similar to what shown by histological sections of two treatments by the first and second ligands. The liver tissue of the group treated with metronidazole showed the occurrence of radially arrangement obviously hepatic cells around the central vein, and liver cells with nuclei appeared clear and centralized with esinophilic cytoplasm, and hepatic cells proliferative as some binucleated hepatocytes, with a simple expansion to sinusoids and the proliferation of kupffer cells, the treatment groups with two ligands marked by the presence of a small clot within the central vein of the liver as well as reported in the treatment group with metronidazole. Tissues sections of the spleen tissue of infected mice treated group by metronidazole showed a clear proliferation of white pulp, which appears containing arterioles clear, white pulp is also surrounded by proliferation red pulp. And showed tissue sections of the group of infected animals and treatment by the first and second ligands as well as the proliferation of lymphocytes, with the presence of megakaryocytes.Showed the three groups non - infected by the first and second ligands and dimethyl sulfoxide, the lack of side effects on each of the cecum tissue, liver and spleen, except for a very simple infiltration of inflammatory cells from macrophages in the cecum of the group treatment by the second ligand
الصفحات الاولى:
مكان الجامعة: ذي قار
اللغة: العربية
الدرجة: ماجستير
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: فاضل عباس منشد العبادي حيدر عباس مهدي الحسيني
اسم المؤلف: عبد الجليل عزيز كريم

تاثير المدعمات المختلفة في حاصل ومكونات الفطر الغذائي Agaricus bisporus واختبار كفاءته في عدد من المتغيرات الكيموحيوية والفسلجية في اناث الجرذان Effect of Various Supplementation in The Yield And Compositions of An Edible Mushroom Agaricus Bisporus And Evaluation of Its Efficiency in Some Biochemical And Physiological Parameters in Female Rats

المستخلص: اجريت الدراسة في كل من مزرعة العرهون التابعة لقسم وقاية النبات - كلية الزراعة ومختبرات قسم علوم الحياة - كلية التربية للعلوم الصرفة في جامعة تكريت خلال المدة 1/9/2013 - 10/6/2014 تضمنت دراستين الاولى تم فيها تقدير تاثير اضافات متنوعة من المدعمات الكاربو | The study was conducted in each of the Al - Mushroom farm in Department of plant protection, College of Agriculture and the laboratories of Biology Department - College of Education for Pure Science at the University of Tikrit for a duration of 1/9/2013 to June 2014. The first studies were included the adding of carbon, nitrogen and other additives at casing period on productivity, quality characteristics and chemical composition of Agaricus bisporus mushroom. The results showed significant increased (p<0.05) in total production and biological efficiency when adding carbon supplement at 1% of starch and became 26.41 kg/m2 and 88.03% respectively, also with nitrogen supplement at 1% from Erbil feed (EF) that’s became at 23.20 kg/m2 and 77.33% respectively, compared with the same value in control group which at 21.68 kg/m2 and 72.26% respectively. Also both treatments were recorded earlier in production at 15 and 16 days after casing compared with 18 days in a control, while the qualitative characterizes has been the treatment of 3% from methionine the highest content of total antioxidants and became at 1975 µg/g dry weight compared to the 1572 µg/g dry weight in the control treatment, and the highest protein content was became at 40.32% in treatment of 1% of LB compared with 22.36% of their content in the control treatment. The treatment of 1% of EF was recorder as higher content from mineral of calcium, magnesium and iron at 390, 2574, 50.74 µg/g dry weight compared to the content in the control group at 290, 1683 and 37.64 µg/g respectively, and the treatment of sodium selenite 5% was recordes a higher content of selenium at 5.00 µg/g compared to the control treatment which at 4.00 µg/g dry weight. The treatment of EF also recorded a higher copper content at 108.60 µg/g dry weight compared to the control treatment which at 37.00 µg/g. The other study was apply to assessment the effects of replacing casein at 5, 10 and 15% by the Agaricus bisporus product from the treatments with starch and multi - vitamin and EF on some growth and physiological parameters in female rats feding for 28 days. The results showed that the presence of mushroom in food has caused a significant decline in weight. The present of mushroom powder at 5% in rats feed lead to significant increasing in hemoglobin and total account of RBC resulting in 12.53 and 6.36× 106 cell/mm3, 13.13 and 7.06× 106 cell/mm3, 7.36× 106 cell/mm3 for starch, multivitamins and EF, respectively, compared to 12.25 and 6.8× 106 cell/mm3, respectively. As well as the significant increased in the total counts of white blood cells that were at 5.50, 5.20 and 5.00 x 103 cells/mm3, respectively, with the starch, and at 5.70, 5.40 and 5.20 x 103 cells/mm3, respectively, with multivitamins and at 5.90, 5.50 and 5.40 x 103 cells/mm3, respectively, for the EF group compared with the control group were at 5.15 x 103 cell/mm3. also was significant increased in differential WBCs as lymphocytes with increasing the replacing percentage for treatments above. Also due the replace of casein by mushroom on proportions referred to in the above change in blood biochemicals parameters which significant decreased in urea, total cholesterol, triglycerides and low density lipoproteins and increase the concentration of high density lipoproteins, as well as reduced the activity of enzymes Alanine amino transferase (AST), Aspartic amino transferase (ALT) and Alkaline phosphatase (ALP) with increasing the percentage of replacing. As for the effect of replacing the same proportions of the mushroom instead of casein on growth and physiological characteristics of female rats with experimental diabetes induced by Alloxan and fed for 17 days. The results showed that the development of diabetes trial led to a reduction in the rate of animal weights and an increase in the relative weight of internal organs such as the heart, liver, spleen, pancreas and kidneys of infected sugar group, and became at 0.46, 4.39, 0.60, 0.46 and 1.19 g/100 g respectively when compared with the control animals, at 4.07, 0.42, 0.39, 0.23 and 1.04 g/100 g, respectively. And decreased of red blood cells that were at 5.93 x 106 cells/mm3, respectively, for the group affected by diabetes, while at 7.36 x 106 cells/mm3 respectively the healthy control group. And decreased WBCs in the infected animals with diabetes trial and became at 4.83 x 106 cells/mm3 compared with the healthy control group which at 7.03 x 103 cells/mm3. Also there was caused in significant increased in glucose concentration, which was on the first, 7th, 14th, and 17th days at 428, 405, 431.60 and 432.20 mg/100 ml respectively, in comparison with a control without diabetis that was at 123.33, 124.66, 126.33 and 125.33 mg/100 ml, respectively. Further to increased concentrations of urea, cholesterol, triglycerides and low density lipoproteins and a decrease in the values of high density lipoproteins also, increased the values of enzymes activity of each Alanine amino transferase(ALT), Aspartic amino transferase (AST) and Alkaline phosphatase (ALP) compared with the non - diabetic infected control group. That feeding female rats of experimental diabetes on the replacement rates of the mushroom instead of casein in their diet has led to the significant decline of the weight and relative weight of internal organs such as the heart, liver, spleen, pancreas and kidneys compared with animals infected with the induced diabetes with increasing rates of substitution. The results also showed that increased in total counts of red and white blood cells that was at 6.40 x 106 cell/mm3 and 5.00 x 103 cell/mm3 respectively when replacing 5% casein from mushroom compared to the infected control group were at 5.93 x 106 cell/mm3 and 4.83 x 103 cell/mm3 respectively. The substitution of casein with 5, 10 and 15% from mushroom was caused to significantly lower of glucose values to became 273.40, 251.60 and 302 mg/100 ml compared with control group infected which at 432.20 mg/100 ml. Also, because of the found of mushroom in the food was caused a changed in the biochemical blood, which significantly reduced the concentration of urea, total cholesterol, triglycerides, LDL and increase the concentration of HDL. As well as significantly reduced the enzymes (AST), (ALT) and (ALP) activity with the replacement rate increase compared with values in the animals infected with diabetes induced by Alloxan. The results concluded that the possibilty of use starch and EF at 1% for the commercial mushroom production. Mushroom also can be used as a food for lowering blood glucose, cholesterol, triglyseride, LDL and liver enzymes with increase in HDL for the people suffered from diabetes, obesity and heart diseases.
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: النبات
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: كركز محمد ثلج الجبوري عبد الله عبد الكريم حسن
اسم المؤلف: جمال جليل احمد البياتي

دراسة التغايرات النسجية والمناعية لمرضى اللشمانيا الجلدية في محافظة ديالى Study The Histological And Immunological Variation of Cutaneous Leishmaniasis Patients In Diyala Province

المستخلص: اجريت الدراسة الحالية على المرضى المصابين بداء اللشمانيا الجلدية والمراجعين لبعض المستشفيات والمراكز الصحية في محافظة ديالى للمدة من تشرين الاول 2013 ولغاية نيسان 2014. اذ بلغ عدد المصابين باللشمانيا الجلدية 115 مصابا وبمختلف المراحل العمرية يضاف لهم 25شخ | The present study was conducted on patients that infected with cutaneous leishmaniasis reviewing to some hospitals and health centers in Diyala province for the period from October 2013 to April 2014.The number of patients with cutaneous leishmaniasis was 115 in different ages in addition to them 25 person as a control group (uninfected). The proportion of infected women with cutaneous leishmaniasis ulcers was higher 56.522% than males 43.47%, the age group ten years and less was more group that being infected with rate 47.83% while the age group that rang from 71 to 80 was the less categories being infected in percentage 0.87% with a significant differences in the level of improbability less than 0.05. The presence of multiple ulcers was high 71.304% than single ulcers 28.696%. The presence of ulcers that length 1 cm and less (64%) more than other with more than 1 cm (36%) with statistically significant. The cutaneous ulcers were distributed on different areas of the body, the injuries on face was more 36.242% with anumber of 54 cases followed by upper limbs in rate 33.557% with anumber of 36 cases, the sore shoulder came latter with apercentage 1.342% in a significant differences at the level of improbability less than 0.05. The percentage incidence of cutaneous leishmaniasis was rise in kids and students in rats 33.043%, 39.130% respectively in a comparison with earners, housewives and of officers their percentages were 24.349% and 3.478% consecutively in statistically differences at 0.05 probability. The diagnosis of cutaneous leishmaniasis infections was done depending on clinical diagnosis based on the characteristics of the morphological and pathological ulcers, culture methods, impression smears and histopathological diagnosis. Clinical diagnosis for the cutaneous ulcer was recorded 100% percentage in comparison with culture method 10.435% versus 74.782%. percentage to positive smears, while the result of positive histopathological diagnosis was 88.571% on vision of promastigote of Leishmania parasite on semisolid medium while a adoption of existence amastigote from as appositive result of smears and histopathological method of the tissue biopsies taken from ulcer. The histopathological method was high efficiency supported clinical diagnosis when compared to other methods this rise in the diagnosis ratio has been demonstrated in significant differences in statistical probability less than 0.05.A study of amain impacts of histopathological changes in cutaneous ulcers on the infected skin layers at the level of light microscopy was also conducted. These pathological effects were in epidermis of skin including hyperkeratosis, pseudoepitheliomatous epidermal changes, ulcers and emergence of a dark colour, thickening in this layer accompanied suppurations and desquamation of chronic cases. As for the dermis layer, the inflammatory cells were infiltrate include plasma cells, macrophages, lymphocytes and eoisophils, disturbance in the order of layers especially in acute ulcers was taken, while the pathological effects were more in chronic ulcers as observed granulomatous inflammation and necrosis. The amastigote from were presence inside and outside the phagocytic cells.Aswell as some immunological changes that associated with the infection were studied in patients therapists by Pentostame (Sodium Stubogluconate) medication and uninfected control group by measuring the levelsof some cytokines IFN - ?, TNF - ?, IL - 12, IL - 17A and the chemokine MCP - 1. The level of IFN - ? was elevated in serum of cutaneous leishmaniasis patients and reached to 54.118±1.740 pg/ml, then the level was decreased during therapy and recorded 34.421±0.984 pg/ml after 1 - 3 dose a then dropped more after 4doses and more 22.489±0.795 pg/ml compared to uninfected control group 5.092±0.805 pg/ml. As well as the case of TNF - ? which rose their concentration in serum of CL patients and reached to 98.145±2.925 pg/ml, then decrease after treatment 71.507±2.901 pg /ml, 48.381±2.379 pg/ml after 1 - 3dose and more of treatment respectively, while the mean of TNF - ? in control group was 13.173±1.388 pg/ml.An increase in the level of IL - 12 was observed in the group of CL patients 845.598±16.273 pg/ml then the level was dropped during treatment and recorded 619.164±20.209 pg/ml, 495.578±27.038 pg/ml respectively but in control the concentration was 160.222±11.761 pg/ml. IL - 17A level was elevated in CL patients 227.268±5.027 pg/ml and decreased during therapy reach to 170.512±5.481, 126.443 ± 5.565 pg/ml after 1 - 3 doses and four doses and more respectively. The concentration of MCP - 1 chemokine was increased in CL patients 111.0397± 6.985 pg/ml then dropped during treatment 71.084± 2.636 pg/ml after 1 - 3 doses then dropped more 49.543± 2.167 pg /ml after four doses and more while they were in control 12.268 ± 1.609 pg/ml. The results of statistical analysis showed significant differences between these cytokines and their groups at the level of probability less than 0.05.
الصفحات الاولى:
مكان الجامعة: ديالى
اللغة: العربية
الدرجة: ماجستير
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: اسراء قاسم العبيدي
اسم المؤلف: منى حميد احمد العزاوي

التاثيرات الفسلجية والكيموحيوية لعدد من المستخلصات النباتية في الدم والجهاز التناسلي الذكري في الجرذان البيض Rattus norvegicus المعرضة للكرب التاكسدي A Study of Physiological And Biochemical Effects of Plant Extracts on Blood And Male Reproductive System of Albino Male Rats (Rattus Norvegicus) Exposed To Oxidative Stress

المستخلص: تضمنت الدراسة الحالية معرفة بعض التاثيرات الفسلجية والكيموحيوية للمستخلصات المائية للكرفس (Apium graveolensL) والجرجير(Eruca sativa Mill) والطماطم (Lycopersicon esculentum Mill) في الدم والجهاز التناسلي لذكور الجرذان البيض المعرضة للاجهاد التاكسدي المستحد | The aim of the present study was to investigate some physiological and biochemical effects of the aqueous extract of celery (Apium graveolens L.), gardeen rocket (Eruca sativa Mill) and tomato frait (Lycopersicon esculentum Mill) on blood and male reproductive so system of Albino rats (Rattus norvegicus) exposed to oxidative stress induced by hydrogen peroxide 0.5% with drinking water for 21 days using vitamin E, as well known antioxidant as criterion for comparison. For the purpose of this study, preliminary experiments were carried out to find the most effective doses of plant extracts and it was found that these doses were 100, 150, 250 mg/kg body weight for, tomato, celery and gordeen roket extracts respectively. Fifty rats (12 - 14 weeks age) were randomly divided to ten groups (treatments) each with five replicates.These groups are : Group (1) (control group) : Rats without any treatment.Group (2) : Rats treated with H2O2.Group (3) : Rats treated with celery extract (150 mg/kg b.w.).Group (4) : Rats treated with celery extract (150 mg/kg b.w.) and H2O2.Group (5) : Rats treated with Eruca extract (250 mg/kg b.w.).Group (6) : Rats treated with Eruca extract (250 mg/kg b.w.) and H2O2.Group (7) : Rats treated with tomato extract (100 mg/kg b.w.)Group (8) : Rats treated with tomato extract (100 mg/kg b.w.) and H2O2.Group (9) : Rats treated with vitamin E (500 mg/kg b.w.).Group (10) : Rats treated with vitamin E (500 mg/kg b.w.) and H2O2 Results could be summarized as follow : 1. Compared with the control, the oxidative stress induced by H2O2 caused significant increase in total count of leukocytes, neutrophilis, acidophils, basophilis, monocytes and lymphocytes, and in the level of blood sugar (glucose), Total cholesterol, triglycerides(TG), low density lipoprotein (LDL - C), very low density lipoprotein (VLDL - C) atherogenic index (First and second), Urea, Malondialdehyde (MDA) and percentages of dead sperms and abnormal sperms.This stress showed as well, a significant decrease in body weight, Hb, PCV, high density lipoprotein (HDL - C), total protein, Albumin, glutathione, sperm total number, percentages of motile and normal sperms, testes weight, prostate and epididymis weights and seminiferous tubules diameter. This treatments, however, showed no significant variations in activity levels of ALT and AST enzymes and seminal vesicle weight.2. In comparison with treatment (2) (rats treated only with H2O2) , the treatment of rats, under oxidative stress with celery extract showed a significant decrease in total count of leukocytes neutrophils, lymphocytes and in blood suger, cholesterol TG atherogenic (First index) blood urea, MDA, Percentage of dead and abnormal sperms and seminal vesicle weight.This treatment showed also a significant increase in level of Hb, PCV., body weight, total protein, Albumin glutathione, number of sperm, percentages of motile and normal sperms, testis weight, epididymis weight and prostate weight. However no significant variations were detected in levels of acidophils, basophils, monocytes, HDL - C, atherogenic (second index) and seminituferous tubules diameter.3. Treatment of Eruca extract and compared with the treatment of H2O2, showed significant decrease in lymphocytes number and in the level of blood sugar, cholesterol (T.G), LDL - C, VLDL - C and atherogenic (first and second index) blood urea, MDA, percentage of dead and abnormal sperms and seminal vesicles weight. The treatment showed also a significant increase in body weight, numbers of acidophils, monocytes, basophils, HDL total sperm numbers, percentages of motil and normal sperms and weights of testis, epididymis and prostate.However, no significant variations were observed in Hb, PCV, Leukocyte total counts, neutrophil, albumin, ATL and AST and seminiferous tubule diameter.4. The treatment of tomato extract showed similar results to that of celary and Eruca extracts except, a significant increase in AST enzyme and seminiferous tubules diameter was detected in case of tomato extract (i.e not seen in case of other plant extracts).5. Compare with H2O2 treatment, rats under oxidative stress treated with vitamin E (500 mg/kg b.w) showed significant decrease in lymphocyte numbers, Total cholesterol, TG, LDL - C, VLDL - C, atherogenic (first and second index), urea, MDA and percentages of dead and abnormal sperms.At the same time the vitamin E treatment showed a significant increase in body weight , Hb, neutrophils, acidophils , monocytes, total protein, glutathione, total sperms number, percentage of motile and normal sperms, and weights of testis, epididymis, and prostate.However, this treatment (compared with H2O2 treatment) showed no significant difference in the level of PCV total count of leukocytes, basophils, blood sugar, HDL - C, albumin, ALT, AST, seminal vesicle weight and seminiferous tubules diameter 6. The most important histological changes exhibited by the animals under oxidative stress were apparent interstitial degeneration, necrosis of supporting and spermagonial cells, separation of these cells in semicyclic manner from the basement membrane, decrease in spermatids (spermatoblasts) and mature sperms in seminiferrous tubule cavity.These effects (due to H2O2 treatment) were decreased to minimal levels when the H2O2 treated rats exposed to plant extracts and vitamin E, used in this study.7. Animals treated with plant extract or vitamin E alone (i.e not treated before with H2O2) showed no clear difference from the control animal (without any treatment) though some improvement was observed in some physiological, biochemical and histological characters of blood and male reproductive system of the studied animals The above results revealed that the plant extract used in this study play an important role in decreasing the harmful effect of the free radicals in the animals (rats) studied.
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: الحيوان
السنة: 2008
الموضوع العام: علوم الحياة
اسم المشرف: زيد محمد مبارك المهداوي صالح محمد رحيم العبيدي
اسم المؤلف: صاحب جمعة عبد الرحمن

دراسة جزيئية ومناعية لطفيلي اللشمانيا الجلدية Cutaneous Leishmania في المحافظات الوسطى والجنوبية Molecular And Immunological Study of Cutaneous Leishmania In The Middle And Southern Provinces

المستخلص: The study includes distribution of the Cutaneous Leishmania in some governorates in Iraq. Specimens are included cases of outpatients in hospitals like : Al - Karamah, Al - Sadir Teaching Hospital in Baghdad, Al - Hilla Teaching Hospital, Al - Hussein Teaching Hospital and Ein Altamer General Hospitals in Kerbala, Al - Sadir Teaching Hospital in Al - Najaf, Al - Karamah and Al - Zahraa Teaching Hospitals in Wasit, Al - Diwanyia General Hospital. Al - Hussein General Hospital in Al - Nasiriya, Al - Smawa General Hospital, Al - Sadir Teaching Hospital and Al - Qurna General Hospital in Al - Basrah during October 1122 to march 1121 Cutaneous Leishmania is consider a major problem that faces the patients because it causes deformities in the infection region. The study includes 330 cases of skin ulcer in which 225 of them were microscopically positive. Parasitic growth was done using two types of cultural media NNN and RPMI - 1640.The results have shown a considerable difference between male and female in which male was significantly exceeding 131) %22.11 (and female was 94) %72.44 (and 139) %72.44 (of outpatients from rural areas increased in numbers from outpatients of urban areas, and only) %12.11(from urban areas.The number of ulcerations in body has exceeded infection, that means more than one ulceration in male 83 (85%) and for female 41 (62%), and concerning infection in three or four ulceration, it is approximately equal and normally distributed on face areas 99 and a percentage of (44%) then lower arms are 92 and a percentage of (40.80%) and the upper parts are 33 and a percentage of (14.66%). Also, one infection was recorded on The study shows that 46 patients in a percentage of (%20.44) having a dry infection; whereas 179 patients (%79.55) having the wet type.For accurate diagnosis of Leishmaniaa species distributed in Iraq, PCR Was used technique and diagnosed two types of Leishmania parasite that causes the illness. A band in a length of 560 bp in 186 samples related to Leishmania major and the band sample was 750 bp related to 39 Leishmania tropica.Through studying the samples in Kerbala City, the number infected samples were 125 for the years of 2010 - 2011 the actuality is (73) infection and (52) infection from 2011 - 2012 in a ratio of (58.4 % and % 72.7 accordingly) in different inhibited areas in the governorate in which (51)) %71.2 (was in Ein Al - Tamir Suburb, and 29 cases of) %11.1 (in Al - Husseinya Suburb, as well as 17 cases of (13.6%) in Al - Hur Suburb, 16 cases) %21.2 (in the south quarters and 7 cases of (5.6%) in the north quarters and only 5 cases of (4%) in the city center.The molecular diagnosis in Polymerase Chain Reaction for Kerbala governorate samples have shown 31) %17.2 (of L.tropica, 22) %24.7(of them were male and 9 of) %4.1 (of them were female. L. major was 94 of) %42.1 (in which 55 of) %77 (were in male and 39 of) %12.1 (were in female.The immunological study for the patients serums with L. major by ELISA technique has significantly shown raised values of IgG and IgM during infection in comparison with the control group, then it declines slowly after treatment in which IgG was in ratio (1811.1 ± 523.1 mg/dl), and IgM was in ratio (166.7 ±23.6 mg/dl). Also, in L. tropica - IgG was in ratio (1722.1 ± 524.0mg/dl) and IgM was in ratio (25.3±182.9mg/dl) in comparison with control specimens, and then the ratios have declined after treatment dosages with Pentostam drug.The cellular dynamics values have shown an abstract increase in which interferon - gamma (IFN - ?) in infected patients with cutaneous L. major were (221.1±2.2mg/dl) which declined after treatment abstractly to (7.12±1.1mg/dl) as well as for L tropica (22.1±7.2mg/dl) which shows no significant abstract difference after treatment with control.An increase appears in cytokine IL - 10 that reached in L.major (122.1±8.2mg/dl) and after treatment was (8.11±2.2mg/dl) and in L. tropica was (222.1±2.2mg/dl) with no significant abstract difference after treatment with control specimen.As a simple attempt to find a vaccine for Leishmania disease a Lipophosphoglycan was isolated and purified as known factor for promastigot stage. The vaccine injected for two groups of Bulb mice in a concentration of purified vaccine (L.majorand Ltropica) to study the immunological response by detecting the Lymphocyte Transformation assay, the Delayed Type Hypersensitivity test and the PhagocytosisIndex.In lymphocyte transformation the ratio of L. tropica was % 7.2 and for L. major was % 21.7 and there is significant difference by P? 0.05 in comparison with control specimen in which its ratio was %7.4, and for Delayed Hypersensitivity test study the average foot thickness injected with vaccine and the other foot which injected with phenol - saline solution - only (1.11 ± 2.12ml) for the first group and (1.21 ± 2.72) for the second group, and for the control group it has registered (1.77± 1.15 ml) with a considerable difference of P? 0.05. By calculating the ratio of Phagocytosis Index cells, it was 17% and 27.6% for the first and second groups while for the control group it has been reaching 9.10%. Therefore, we can get advantage from these values as indicators for the rule of vaccine in the immunological response and triggering of vaccine idea for this disease.
الصفحات الاولى:
مكان الجامعة: كربلاء
اللغة: العربية
الدرجة: دكتوراه
الموضوع الدقيق: علوم الحياة
السنة: 2015
الموضوع العام: علوم الحياة
اسم المشرف: علي حسين الكبيسي مهدي حسين العمار
اسم المؤلف: ازهار موسى جعفر الموسوي

عزل وتشخيص البكتريا العنقودية السالبة لانزيم الخثرة من حالات مرضية مختلفة ودراسة مقاومتها للمضادات الحيوية Isolation And Identification of Coagulase Negative Staphylococci From Different Clinical Cases And Study of Their Antibiotic Resistance

المستخلص: جمعت 265 عينة من مستشفى تكريت التعليمي العام وشملت هذه العينات عينات سريرية لمرضى راقدين في المستشفى ومرضى مراجعين لها. اخذت العينات من المرضى المشتبه باصابتهم بخمج السبيل البولي وخمج الجروح والحروق وخمج الاذن الوسطى ولكلا الجنسين وباعمار مختلفة. انجزت هذ | A number of 265 samples had been collected from Tikrit Teaching Hospital (T.T.H.).These samples were taken from inpatients and outpatients suspected to have Urinary Tract Infection, Wound & Burn Infections and Mid - Ear infection for males and females in varied ages. The practical part had been fulfilled during the period from the beginning of December 2006 till the beginning of March 2007.Isolates diagnosed were identified depending on morphological and cultural characteristics. The results revealed 60 Staphylococci isolates, which shows 27 isolates (45%) were S. epidermidis, 13 isolates (21.66%) S. saprophyticus, 10 isolates (16.67%) S. xylosus, 3 isolates (5%) S. lentus, two isolates (3.33%) S. heamolyticus, and one isolate (1.67%) for each of S. simulans and S. hominis Ten (34.48%) of patients are located in the age group (15 - 30) years. while concerning the classification according to sex, females were (65.5%). Otitis media and Wounds and Burns infections, their ratio was high (61.1%, 53.8%) consequently both in males in comparison with females. Concerning the relationship between the infection and the residence and the level of education, the results revealed that isolation of Coagulase Negative Staphylococci (CoNS) were high in rural areas and in uneducated persons regarding the various infections than those live in urbans and educated ones. A of main tests were carried out in order to achieve the purpose of this study according to the planned goals, some of which are the virulence factors of Coagulase negative Staphylococci. These include DNase, Urease, Lipase, Lecithinase, and Alkaline phosphatase.The results revealed the ability of 67.5% of isolates to produce Alkaline phosphatase, and 46.6% of isolates to produce Urease, also 27.5% of isolates were able to produce Lecithinase , and the ability of 19.16% to produce Lipase, while 22.5% of isolates were capable of producing DNase. In addition to these tests, the test for producing ? - lactamase was done, 85% of isolates were positive to this test because ? - lactamase is one of the significant enzyme which are produced by bacteria since it improves the resistance of bacteria for antibiotics. Thirteen different antibiotics used for the Antibiotics susceptibility tests which show that most effective antibiotic is Nitrofurantoin (95%), followed by Amikacin (93.3%), Novobiocin(90%), Chloramphenicol(88.3%), Rifampicin (78.5%), Vancomycin (75%), Ciprofloxacin (73.3%), Clindamycin (70%), Gentamicin (58%), Penicillin G (50%), Trimethoprim (50%), Tetracycline (40%) and Cefoxitin (18.3%).
الصفحات الاولى:
مكان الجامعة: صلاح الدين
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: التقنيات الاحيائية
السنة: 2008
الموضوع العام: علوم الحياة
اسم المشرف: عباس علي هادي الدجيلي وعد محمود رؤوف
اسم المؤلف: مجاهد خلف علي احمد الجميلي

تاثير فيتامين E والسلنيوم على الجرذان المحقونة بالذيفان الداخلي لجراثيم Salmonella typhimurium Effect of Vitamin E And Selenium On Rats That Injected With Salmonella Typhimurium Endotoxin

المستخلص: صممت الدراسة الحالية بهدف تحديد التاثيرات الفسلجية والمناعية والنسجية المرضية التي تسببها الذيفان الداخلي لجرثومة Salmonella typhimurium وتداخل فيتامين E والسلنيوم في تقليل تاثيراتها السمية في اعضاء الجسم وتعزيز فعالية الجهاز المناعي لذكور الجرذان البيض. | The present study has been designed to determine the physiological, biochemical, immunological and histopathological effects of endotoxin of Salmonella typhimurium and the interaction of vitamin E and selenium in releifing its toxic effects on body organs, as well as its immuno - modulatory effect in mature male ratsForty mature male rats, nearly similar in their weight and age, were randomly divided into four equal groups : First group (control) rats were injected with normal physiological saline (0.5 cc ip) and 2nd group (T1) have been given the ration drinking water and injected with endotoxin intrapretonealy with dose 0.5 mg/kg bw for 18 days while (T2) and (T3) groups were treated by vitamin E with a dose of 30 mg/kg bw and selenium with a dose of 2.8 ppm/kg bw) for 30 days. (T2) was injected at thirty one day by endotoxin at a dose 0.5 mg/kg bw intrapretonealy for 8 days. Public health parameters like body weight, blood parameters (RBC count, PCV, total WBC count and differential leucocytes count, AST and ALT concentration) Phagocytosis activity for neutrophil and monocytes. Immunoglobulins (IgA, IgM and IgG) estimation, gross and histopath - ological changes and clinical sings were included in the present study.The results revealed that endotoxin injection led to different changes, its significancy flactuated according to the type of studied parameter, as follow : The results of clinical sings of experimental animals revealed occurrence of excitation after 10 minutes of injection, sever dullness, loss of appetite, muscular weakness, lateral recumbency in addition of loss of weight and easily handle. Significant decrease (P<0.05) of body weight, RBC count, ,PCV and monocytes percentage. Significant increase (P<0.05) of total WBC count and percentage of neutrophil, lymphocytes, eosinophil, and AST, ALT, IgM and IgA concentrations and no significant difference of IgG concentration. Significant decrease (P<0.05) of phagocytic activity.Gross pathological changes represented by the presence of necrotic and hemorrhagic foci, clotting as well as congestion and enlargement of liver, kidneys, lungs, spleen and heart. The histopathological appearance revealed by vaculation, necrotic and degenerative changes of hepatocytes in live, the spleen showed many changes led to imbalance between white and red bulb spaces, the heart showed lack of cross striation, edema between cardiac muscle fibers, while lungs showed an emphysema and sever hyperplasia in the bronchial epithelium. From other hand, the results showed that receiving vitamin E and selenium had an efficient effect for the improving the toxic effects of endotoxin and relieving its toxicological changes in the animal organs as compare with group that injected with endotoxin only, this group shows slight clinical signs and increase of appetite and drinking of water, improvement of blood parameters and reduce in the concentration of ALT and AST in blood serum, also there was increase in the phagocytic activity and increase in the levels of IgM, IgA and IgG. The gross pathological cganges less sever as compare with T1. It can be concluded that exposure to Salmonella typhimurium endotoxin leads to many distinct toxic effects on different organs and systems of the body which may results in many physiological disturbances. This study, also, proved that vitamin E and selenium have an important efficient role in improving the toxicological changes resulted from endotoxin poisoning.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الطب الباطني والوقائي
السنة: 2010
الموضوع العام: الطب البيطري
اسم المشرف: جبار عباس احمد الساعدي محسن عبد نعمة الروضان
اسم المؤلف: زينة حمزة جعفر

دراسة تاثير بعض الاضافات على الاستجابة المناعية ووزن الجسم والتغيرات النسجية لفروج اللحم الملقح بلقاح مرض النيوكاسل A Study The Effect of Some Additives On Immune Response And Body Weight And Histological Changes For Broiler Chicks Vaccinated With Newcastle Disease Vaccine

المستخلص: اجريت? هذه الدراسة لمعرفة تاثير بعض الاضافات في الاستجابة المناعية والوزن والتغيرات النسجية في الامعاء الدقيقة. حيث تم تقسيم (350) طيرا من فروج اللحم بعمر يوم واحد من نوع Ross 308) ) غير مجنسة , وزعت عشوائيا الى (7) معاملات تغذوية متساوية وهذه المعامل | This study was conducted to investigate the effects of feed additives on immune response , weight and histological changes in small intestine. A total of (350) broiler chicks one - day old (Ross 380) - unsexed were randomly divided in to (7) equal dietary treatment groups , as fallows : Treatment (T1) : Basal diet without any addition ( control group).Treatment (T2) : Basal diet + Garlic 4cc/10 LTreatment (T3) : Basal diet + Saccharomyces cervisiae 2.5gm/10 L.Treatment (T4) : Basal diet +Stpire 2.5cc/10 L.Treatment (T5) : Basal diet +B. glucan 8gm/10 kgTreatment (T6) : Basal diet + Levamisole 1gm/kg.Treatment (T7) : Basal diet + Neomycin 0.5 gm/L.These additions were presented on the first day of the arrival for 5 days, and on the day of first vaccination for 5 days and at the end of experiment for 5 days also. This chicks vaccinated with NDV on the day one of age by injection in the neck and re - vaccinated at day 10 of age with LaSota, Body weight were weekly determined. ,blood samples were collected from wing vein for ELISA test in ( 4,18,27 ) days old respectively. The results as following : - 1 - Treatment of levamisole (T6) were significantly increased ( p<0.05) in (T2, T5) in level of Abs production against ND virus.2 - Chicks feed diet with saccharomyces cerevisiae showed significantly increased ( p<0.05) in body weight among all experimental group , and improvement in weight in (T6,T7) with other group.3 - Chicks feed diet with (T7) showed improvement in villus hight and in the ( T3, T4).
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الامراض - الدواجن
السنة: 2015
الموضوع العام: الطب البيطري
اسم المشرف: نافع صبيح جاسم
اسم المؤلف: هبة تركي عطية الرماحي

دراسة التاثير العلاجي لحليب الافراس المخمر للتقليل من تاثير الاجهاد التاكسدي المستحدث بواسطة خلات الرصاص في الجرذان البيض Study The Therapeutic Effect of Fermented Mare? S Milk To Reduce The Effect of Oxidative Stress Induced By Lead Acetate In White Albino Rats

المستخلص: اجريت الدراسة الحالية في البيت الحيواني التابع لكلية الطب البيطري / جامعة القادسية بهدف تقييم الدور العلاجي لحليب الافراس المخمر في التقليل من تاثير الاجهاد التاكسدي المستحدث بواسطة خلات الرصاص. استخدم في هذه الدراسة ( 40 ) ذكر من الجرذان البيضاء نوع ( w | The present study was conducted at the animal house of veterinary medicine college of AL - Qadisiyah University during the period from 27 - 2 - 2014 to 30 - 3 - 2014. to evaluate the productive role of fermented mare's milk in to reduce the oxidative stress that induced by lead acetate , 40 male albino rats weighing between 200 - 210 gm were used in this study. the animals were obtained from the animal house of veterinary medicine College / Baghdad University. The animals were randomly distributed into four equal groups , ( 10 rats per each group / cage) as follow : - Control group : The rats were given only basal diet and deionized drinking water - - First treatment group ( T1) : The rats were given basal diet and deionized drinking water that contain 1000 ppm lead acetate for 30 days. - Second treatment group ( T2 ) : The rats were administrated basal diet and deionized drinking water that contained 1000 ppm of lead acetate and received orally ( by gavag ) fermented mare's milk at a dose ( 10ml / kg b.w / day ) for 30 days. - Third treatment group (T3) : The rats were given basal diet and deionized drinking water that contained 1000 ppm of lead acetate and received orally ( by gavag ) EDTA at a dose ( 50 mg/ kg b.w / day ) for 30 days. At the end of the experiment all the animals were fasted for ( 12 hours ) and then killed animals from each treatment group blood samples were collected from the heart to measures some blood parameters such RBC , Hb and PCV and biochemical parameters such as liver function parameters ( total protein and activity of some enzymes such as ALT , AST , ALP and LDH ) , kidney function parameters such as Urea and Creatinine concentration and oxidative stress parameters such as GSH , SOD , MDA ). In addition to that study the histopathological changes of liver and kidney tissues were studied The results of the present study can be summarized as follow : - 1 - Blood parameters : The results of the statistical analysis showed that there was a significant ( p < 0.05 ) decrease in the mean values of RBC , Hb , and PCV in a group treated with lead acetate only ( T1 ) as compared to other experimental groups. while groups treated with EDTA and fermented mares milk showed a significant ( p < 0.05 ) improvement in blood parameters. 2 - Biochemical parameters : A - Liver function parameters : The result revealed that there was a significant ( p < 0.05 )effect of lead acetate on level of total protein where caused a decrease in total protein concentration and increase the in activity of ALT , AST , ALP , and LDH as compared to other experimental groups. Yet treatment with fermented mare's milk ( T2 ) resulted in an increase in the total protein concentration and a decrease in the activity ALT , AST , ALP AND LDH. Also treatment with EDTA ( T3 ) caused a similar results to ( T2 ). B - Kidney function parameters : The results obtained in this study demonstrated that a significant ( p < 0.05 ) increase in the level of urea and creatinine in a group of animals treated with lead acetate only ( T1) , while using fermented mare's milk ( T2 ) and also EDTA contributed reduction in the level of urea and creatinine 3 - Oxidative stress parameters : The results of the present study showed that there was a significant ( p < 0.05) decrease in the concentration of GSH and SOD a significant ( p < 0.05 ) increase in MDA concentration in animals that administrated lead acetate only ( T1) while administration fermented mare's milk and EDTA resulted in significant ( p < 0.05 ) increase in the concentration of GSH and SOD while a significant ( p < 0.05 ) reduction in MDA concentration. The values becomes close to the concentration of the control group. 4 - Histopathological changes : The results of Histopathological examination for tissues of liver and kidney of rats given lead acetate alone ( T1 ) showed that there was a clear Histopathological changes presented in degeneration of hepatocytes and congestion in central vein which associated with hemorrhage in liver and kidney tissues of animals administrated fermented mare's milk showed normal and free from congestion.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الصحة العامة
السنة: 2015
الموضوع العام: الطب البيطري
اسم المشرف: كريم ناصر طاهر
اسم المؤلف: شيماء عباس صبيح

دراسة الاهمية الوقائية لحليب الماعز وتاثيراته في بعض الصفات الانتاجية والمناعية لاناث الارانب المعاملة بالمضاد الحيوي اموكسي كلافولانيك اسيد The Important Protective Study of Goat Milk And Its Effects On Some Productive And Immune Traits of Female Rabbits Treated With Amoxi - Clavulanic Acid

المستخلص: This study was carried out from 12 - 3 - 2011 to 29 - 4 - 2011 to find out the protective importance of goat milk and its effects on some productive traits ( body weight , weight gain , feed intake, and feed conversion efficiency) ,some immunological traits ( white blood cells , percent of lymphocyte cells) ,some biochemical traits (total serum protein , albumin ,globulin ) ,some physiological traits ( hemoglobin and Packed cell volume) in the rabbits treated with Amoxicillin - clavulanic acid.A total of (32) female rabbits (local breed) aged range between 2 - 3 months with average of initial body weight 1706.25 gm.were distributed randomly into four groups each one consist of eight rabbits as below : 1 - Control group ( C ) : Fed basal diet without any addition.2 - First treatment group ( T1 ) : Fed the same basal diet + 5 mg ? kg b.w. x 2 amoxi - clavulanic acid for one week3 - Second treatment group ( T2) : Fed the same basal diet +( 5 mg ? kg b.w. x 2 amoxi - clavulanic acid + 5ml x2 raw goat milk ) for one week.4 - Third treatment group ( T3 ) : Fed the same basal diet + 5ml x2 raw goat milk for one week.The experiment continued for five weeks in which each rabbit weight at weekly interval and the residual feed weighed daily ,The results revealed the following : - Treatment one showed signs of gastric ulcer ,diarrhea , small and large intestinal inflammation ,while T2 group ( antibiotic + goat milk ) did not show any signs of gastric ulcer, diarrhea and their digestive system normal like the control ( C ) and Treatment three ( T3 ) group ( goat milk ).Body weight of ( T3 ) group recorded significantly) P < 0.05 )increased as compared with body weight of T1 group in all study weeks. And recorded the highest body weight among the other study groups during all periods of the study weeks (1769.12, 1751.37,1795.00 , 1824.25 ,1852.75) gm respectively.Animals in group ( T3 ) exceeded in weekly body weight gain as compared with other treatment groups during the studied period except the second week ( 62.87, - 5.25, 32.5, 29.25 , 28.5) gm respectively.Treatment ( 1 ) showed decrease in weekly feed intake on the first 4 weeks of study ) 60 , 42.51 , 44.88 , 34.25 gm )respectively as compared with C and T3 groups.Treatment ( 3 ) showed that feed conversion efficiency means recorded better value during the fifth weeks of studied weeks and was ( 5.53 , - 64.30 , 10.79 , 11.98, 12.11) gm food ? gm gain respectively recorded.There are significant increases ) P < 0.05 ) in white blood cell means for C ( control ), T2 , T3 groups) 6.352, , 6.32 6.45) x 10 3 ? ?L. compare with the T1 group ( 4.772 x 10 3 ? ?L ) and also lymphocyte percentage means of T1 (38.95 % ) showed significant lower ) P < 0.05 ) compared with other treatment groups ( C , T2, T3 ) ( 55.05 , 54.80 , 57.07 ) respectively.The control and T3 groups showed significant) P < 0.05 ) exceeded in hemoglobin and packed cells volume as compared with T1 and T2 groups which there is no significant difference between them.Where the average of four treatment group control ,T1 ,T2, T3 record 44.837 , 38.675 , 38.825 , 44.800 respectively.Treatment 3 exceeded other treatment groups in the total protein ( 88.156 )gm/l and also T1 group showed significant ) P < 0.05 ) decrease in albumin ratio ( 26.682 ) gm/l, while T1 showed significantly ) P < 0.05 ) higher in their gamma globulin compared with other treatment groups.Treatment 3 and 1 groups showed significant ) P < 0.05 ) increase in liver relative weight as compared with the control and T2 groups and the liver relative weight means of the ( C ,T1 ,T2 ,T3 ) groups as followed respectively 2.64, 3.05, 2.53 , 3.08 %.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الصحة العامة
السنة: 2012
الموضوع العام: الطب البيطري
اسم المشرف: كريم ناصر طاهر علاوي لعيبي داغر
اسم المؤلف: حسين باعي خضر حسين الجياشي

اسباب انخفاض الخصـوبة والعقـم في الافراس العراقية The Causes of Infertility And Sterility In Iraqi Mares

المستخلص: اظهرت نتائج الدراسة الحالية ومن خلال مراجعة المصادر ان مشاكل الخصوبة والعقم في الافراس تتخلص بــ : اسباب خارجية تتعلق بعدم استخدام الافراس خلال الموسم التناسلي بسبب ظروف تصميمية او ظروف لايمكن تجنبها متمثلة بقصر موسم التناسل والولادات في وقت متاخر من الموس
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دبلوم عالي
السنة: 2013
الموضوع العام: الطب البيطري
اسم المشرف: ضياء حسين جاسم الدليمي
اسم المؤلف: سمير صبري كاظم الخفاجي

دراسة مسببات عسر الولادة في الجاموس Etiological Study of Dystocia In Buffaloes

المستخلص: تهدف هذه الدراسة الى معرفة الاسباب الرئيسية لعسر الولادة في الجاموس الذي يؤدي الى حدوث خسائر اقتصادية كبيرة نتيجة لموت المواليد، وزيادة فترة المخاض، زيادة تكاليف العلاجات البيطرية، انخفاض في الاداء التناسلي المستقبلي للاناث المجهضة، احيانا يؤدي الى فقدان | The aim of the present study was to find out the common major causative agents of dystocia. Dystocia causes important economic losses due to fetus death loss ,increased labor ,increased veterinary cost, reduced subsequent reproductive performance of the dam, potential loss of buffaloes, and reduced milk production. Many factors affecting calving difficulty, including birth weight of the fetus, pelvic area of the dam, gestation length,sex of the fetus, body condition of the female at calving, inadequate heifer development , abnormal presentation of the fetus and abnormal hormones levels in the female when giving birth.The major cause of dystocia is a disproportion between the birth weight and dam pelvic area. The study concluded that the selection of sires based on birth weight is a much more effective tool that selection of replacement heifers based on pelvic area to reduce dystocia in first pregnant heifers.The proper feeding and management is the right solution to decrease the incidence of dystocia.
الصفحات الاولى:
مكان الجامعة: البصرة
اللغة: العربية
الجامعة: جامعة البصرة
الدرجة: دبلوم عالي
السنة: 2010
الموضوع العام: الطب البيطري
اسم المشرف: طاهر عبد الحسين فهد
اسم المؤلف: مروة علي هادي الفياض

دراسة تاثير الفصول على بعض الصفات التشريحية والفسلجية للجهاز التناسلي في ذكور الجمال وحيدة السنام (Camelus Dromedarius) Study of The Seasonal Effect On The Some Anatomical And Physiological Features of Parts of The Genital System In Adult Male One Humped Camel (Camelus Dromedarius)

المستخلص: اجريت الدراسة الحالية على (96 عينة) لاجهزة تناسلية (خصى وبرابخ )ودم لذكور جمال ناضجة جنسيا بمعدل (6 - 5سنة) بعد التاكد من سلامتها قبل الذبح وبعده والتاكد من عمرها بواسطة المعادلة السنية من مجازر (النجف وعفك والديوانية ) لمدة سنة كاملة بواقع (8عينة) لكل شه | The current study was conducted on(96 samples) of genital organs (testes and epididymis) as well as blood of male mature camels (5 - 6 years) after the confirmation of their health before and after slaughter, and to make sure from their ages by the dential equation,these sample were collected from abbatoris of AL - Najaf, Afak and Diwaniya) for the full year with (8 samples) per - month for the purpose of determination parameters anatomical parameters for some of the genital system of the testes and epididymis (right and left), physiological parameter (hormonal elements chemical), the study anatomical results biggest weight, length, width and circumference of the lift testis and the weight and length of the left epididymis , mis recorded in winter significally(p <0.05), which reached(90.041 ± 0.613, 103.904 ± 0.633, 50.541 ± 1.957, 256.812 ± 0.615, 40.500 ± 0.288, 109±0.7), respectively, while less weight, length, width, circumference, weight and length of of the testis left and weight length of lift epididymis which recorded in the summer significally (p <0.05)and reached (85.666 ± 0.441, 92 ± 0.357,, 40.583 ± 0.220, 242.266 ± 0.15, 35.083 ± 0.083, 94.34 ± 0.45) respectively, while the measurements were in the weight, length, width and circumference of the right testis and weight and length of the right epididymis was recorded in winter and the level of significance (p <0.05), recorded(89.373 ± 0.474, 101.958 ± 0.622, 48.895 ± 1.181, 256.060 ± o.688, 35.945 ± 2.878,30.728 ± 0.518) respectively, , while the measurements of the less weight, length, width and circumference of the right testis and weight and length of the right epididymis was recorded in the summer in level of significance (p <0.05)and recorded (84.700±0.472,97.314±0.291,39.666±0.707,241.104±0.104,26.395±0.055, 92.5±0.3) respectively, the results of physiological and features related with hormonal the measurements that Folicul stimulate hormone (FSH) and lutyal hormone(LH) and (Testosterone hormone) have recorded the highest concentration in the winter and the level of significance( p<0.05) and recorded (5.128 ± 0.455, 2.770 ± 0.3184, 10 ± 1), respectively, while the lowest levels were recorded in summer with level of significance( p<0.05) and recorded(1.633 ±0.130 IU,2.145 ± 0.13 IU, 3.5 ± 0.091 ng/ml) while prolactin hormone has recorded lowest level of significance (p <0.05) in the winter and the highest level in the summer which recorded (102.2 ± 8.405 IU ,244 ± 24.463 IU)respect ally. the chemical elements increased their ranges in the winter and spring (p <0.05) which reached each of calcium, magnesium, sodium and potassium (8.791 ± 0.122 mg/dl, 3.016 ± 0.130 mg/dl, 147.916±2.061 Mmol/L, 7.000±0.003Mmol/L) and in the spring(8.100±0.52mg/dl,2.279±0.056mg/dl,146.458±3.129Mmol/L,8.405±5.181Mmol/L).while the lowest levels were in summer(p<0.05)which recorded(7.683 ±0.202, 2.236 ±0.148mg/dl,145.454 ±0.275Mmol/L,24.463 ± 5.027Mmol/L) respectively
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
السنة: 2013
الموضوع العام: الطب البيطري
اسم المشرف: ضياء حسين جاسم الدليمي
اسم المؤلف: عماد حمزة مروح

دراسة تاثير برامج الرعاية التناسلية على الخصوبة خلال فترة النفاس في ابقار الحليب Study of The Effect of Reproductive Care Programs On Fertility During Postpartum Period

المستخلص: تهدف الدراسة الى استعراض المراجع المهمة التي تهتم بالرعاية التناسلية لحيوانات المزرعة وخاصة الابقار لما لها من اهمية كبيرة في حيات الانسان حيث شملت استعراض لبرامج الرعاية التناسلية والطرق اللازمة للحفاظ على اعلى انتاج حيواني وكما شملت الدراسة اهم الامراض | This study aimed to review important references which deal with reproductive care of farm animals specially cows because of the great importance in human life. That included the ways to maintain high animal production and it included the most important maternal disease that cause reduction in animal reproductive efficient and perdition or exclusion of animal.The study review predisposing factors and environmental conditions which cause significant economic losses perhaps the nutrition has been consider the most importance factor. And this study also covered the roles of hormones because of the active effect in reproductive efficient and explain each hormone and its affect on the animal body during pregnancy and correlation with other hormones.The reproductive care programs are the most programs which must applied for high animal production and reduce the reproductive disease and treat them with simple ways to decrease economic losses that affect on owner s yield material then affect on national economy directly so shall be taken into consideration. The most importance problems and correlation with good management for high reproductive efficient of farm animals specially dairy cattle. Supervisor's certificate We certify that this study (the study of the effect of reproductive care programs on fertility during postpartum period in dairy cattle) was prepared under our supervision at the college of Veterinary Medicine of AL - Qadissiyah University, as a partial fulfillment of the requirement of the degree of Higher Diploma of Science in Veterinary Medicine
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: دبلوم عالي
السنة: 2013
الموضوع العام: الطب البيطري
اسم المشرف: عبد الكريم محمد جعفر
اسم المؤلف: اسراء راضي لطيف الزبيدي

عزل وتشخيص جزيئي لمورثات مجاميع الانماط المصلية لجرثومة اللستيريا المستوحدة Listeria monocytogenes من الانسان والحيوانات في محافظة القادسية Isolation And Molecular Detection of Serotype Genes Group of Listeria Monocytogenes From Human And Animals In Al - Qadissyia Province

المستخلص: اجريت الدراسة الحالية خلال الفترة من تشرين الثاني 2014 الى نيسان 2015، جمعت العينات المرضية من النساء التي تعاني من حالات الاجهاض وولادة مبكرة والاطفال المصابين بالتهاب السحايا من مستشفى الولادة والاطفال التعليمي وقد جمعت العينات تحت اشراف طبي متخصص وكان | This study was carried out during the period from November 2014 to April 2015, whereas samples were collected from women that suffered of abortion and infected children in the maternal and children hospital in Al - Qadissiya province , so the samples were collected under supervising of a specialist medicine , the number of taken samples of the women is 65 samples , while the number of taken samples of children is 32 samples , so the samples that belong to animals have included a gall bladder samples were collected from Al - Diwaniya slaughterhouse were 100 samples of sheep and 100 samples of cows. in addition to collect 200 samples of milk as 100 samples for each sheep and cows from different rural zones in al - Qadissiya province. L.monocytogenes was isolated by using oxford listeria selective agar for listeria , so the special stimulator for growth of the bacteria was added to it in order to determine the type of bacteria , also it diagnosed an isolates for L.monocytogenes by using the microscopically examination and biochemical tests , furthermore the germ isolates was planted on blood agar for diagnosing the blood analysis type : ? - heamolysis as affirmative checking for the isolates. also PCR technique used for affirming the diagnosing of L. monocytogenes by using the initiator for the blood analysis gene (hly) , whereas all the L. monocytogenes isolates' gave a positive result in inspection , so this technique is used too in the detection about the serous types for L.monocytogenes isolates by the molecular diagnosing for the fierce genes of the germ and these are (D1,D2,Glt,FlaA and MAMA gene ). The results of the research showed that isolating and diagnosing percentage of the Listeria monocytogenes is about 9.27%, whereas the isolation percentage in women infected in abortion is 4.61%, while the percentage of listeria germ isolation was higher in the children that infected in meningitis whereas it was 18.75% of the total amount of the samples.As for the percentage of the isolating in sheep it was 4% and 7% for milk and gall bladder respectively. In the cows the isolation percentage of the germ from milk and gall bladder was 2% and 3% respectively. So the the results of isolating for listeria germ during the study period in human and animals were disparate , whereas the highest percentage of isolation for the germ in human were scored in January and February months of the year whereas it scored 20% and 16% respectively.As for sheep the percentage of isolation was higher in December and January months whereas it is 6.25% and 10% respectively , while in the cows the percentage was higher in January and February months where it is 4.2% and 7.4% respectively. Regarding The results of detection about the genes of the serous types for the isolates of L.monocytogenes by using PCR technique 14 isolates of the samples showed a positive result when they react with the initiator for gene (D1)in percentage about 56%, so this isolates showed a positive result when they react with the initiator for gene (Glt) as amount of 5 isolates and they classified to be belong to the serous types (1/2B or 3B), while 9 isolates gave a negative result in the inspection , whereas these isolates were tested with the initiator for gene (MAMA), 5 isolates showed a positive result in this reaction with this gene and they classified to be belong to the serous types (4c or 4a), while 4 isolates gave a negative result as they classified to be belong to the serous types (4b or 4d or 4c).As for the isolates' that gave a negative result with the initiator of gene (D1)there are 11 isolates all these isolates gave a positive result when they inspected with the initiator of gene (D2) in a percentage about 44%, so when these isolates inspected with the initiator for gene (FLaA) then 6 isolates of them gave a positive result in the inspection and classified as they belong to (1/2a or 3a), while 5 isolates of them gave a negative result in the inspection and classified as they belong to (1/2c or 3c). As for the serous types that have not diagnosed in the germ isolates of the human there are(1/2a or 3c), while in the germ isolates of the sheep the serous types (4a or 4c) have not been diagnosed , then the serous types (1/2b or 3b) have not been scored in the cows. The present study concluded that the percentage of infection in L.monocytogenes was high in human in al - Qadissiya province , in which that refer to the risks of the diseases and health problems spread that could be caused by L.monocytogenes , also the results included isolating the germ from milk and gall bladder samples for sheep and cows that could be a source to carry the infection to the human by contaminating the meat, milk and its derivatives'. whereas the isolation percentage of the germ was higher in the cold months of the year in human and animals, so the highest isolation percentage of the serous types for the L.monocytogenes in human and cows were (4a , 4c) , while in sheep the serous types were (1/3c,3c).
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الامراض المشتركة
السنة: 2015
الموضوع العام: الطب البيطري
اسم المشرف: هدى عبد الهادي علي النصراوي
اسم المؤلف: حسين عمران كريم العابدي

دراسة تاثير الخصي في مستويات بعض الهرمونات التناسلية وبعض المعايير الدمية في ذكور الماعز المحلي الاسود Study The Effect of Castration On The Levels of Some Reproductive Hormones , And Some Hematological Parameters In The Local Black Buck

المستخلص: اجريت الدراسة لغرض التعرف على تاثير عملية الخصي في ذكور الماعز المحلي الاسود في مستويات الهرمونات التناسلية والصورة الدمية , وقد اشتملت الدراسة على (25) ذكرا تراوحت اعمارها بين (1.5 - 2.5) سنة وكانت خالية من الامراض والمشاكل التناسلية , وقسمت عشوائيا ال | The current study was conducted for the purpose of identification the effect of the castration in male goats on levels of reproductive hormones and some hematological parameters , and had included (25) males ranged from ages between (1.5 - 2.5) years old and was free from diseases and reproductive problems. The animals in this study were randomly divided into two groups (control group includes (10) male and experimental group includes (15) male) that housed in same conditions of nutrition and management. The levels of reproductive hormones include (Spermatogenesis Stimulating hormone (SSH) , Interstitial cell stimulating hormone (ICSH), testosterone, Estradiol - 17 beta and progesterone) as well as blood parameters that include (red blood cells( RBCs), total white blood cells ( tWBCs), packed cell volume (PCV), hemoglobin concentration (Hb), total protein and cholesterol concentration) were estimated three times before castration and in intervals of two weeks between examination and other , Experimental animals were examined after castration all hormones and blood parameters checked two weeks after castration, then after four weeks from castration, and re - measured all hormones and blood parameters after six weeks of the castration. The method of analysis Radioimmunoassay (RIA) was used to measure the levels of reproductive hormones in animals blood serums also the routine blood tests were use for checking the blood parameters. The results revealed that there are significant increased in the level of ICSH in experimental animals after castration (0.345 ± 0.0413) mIU / ml while the level of testosterone and estradiol - 17 beta significantly decrease in experimental animals after castration and the results were (0.0225 ± 0.0036) ng/ ml and (0.93 ± 0.15) pg/ ml, respectively. While castration did not show any significant effect on the levels of reproductive hormones SSH and progesterone (0.145 ± 0.01) mIU/ ml and (0.295 ± 0.03) ng /ml respectively in experimental group. The result showed that there were significant increased in RBCs , PCV, total protein and cholesterol (17125 ± 335.18) × 10 3 cell/µL , (37.7 ± 0.40) , (6.84 ± 0.25) g /100 ml and (84.41 ± 6.6) mg/100 ml respectively in experimental group after castration, while tWBCs and hemoglobin didn't affect after castration in experimental group (10788 ± 429.39) cell /µL and (11.35 ± 0.180) g/ 100 ml, respectively.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
السنة: 2011
الموضوع العام: الطب البيطري
اسم المشرف: ضياء حسين جاسم الدليمي
اسم المؤلف: باسم حميد عبد علي

تركيز المعادن الثقيلة في حليب الابقار والاغنام والماعز في محافظـة القادسية The Concentration of Heavy Metals In Milk of Cows , Sheep & Goats In Al - Qadisiya Province

المستخلص: اجريت هذه الدراسة في محافظة القادسية للمدة من كانون الاول 2012 لغاية اذار 2013 شملت خمسة مناطق مختلفة من محافظة القادسية هي كل من (مركز الديوانية وناحية السنية وناحية الشافعية وناحية الدغارة وقضاء الحمزة) لغرض تحديد مدى تلوث الحليب الخام ببعض المعادن | This study was carried out in AL - Qadissiyah province during the period from December 2012 to March 2013 involved five different regions such as (Diwaniyah city center , Saniyah district , shafeia district , Dagharah district and AL - Hamza district) to determine the level of contamination of raw milk with the toxic heavy metals such as Cadmium (Cd), Lead (Pb), Nikel (Ni), Arsenic (As) and Mercury (Hg). A total of 150 raw milk samples were collected from 150 animals belonged to three species involved (50) cows , (50) sheep and (50) goats selected randomly, ten animals per each species per regions. The results of a statistical analysis of the experimental data showed that : Chemical components of milk 1.There are a significant differences (p <0.05) in all contents of milk among different species of studied animals (cows , sheep and goats). Except fat content for cows and goats milk were not significant different. Sheep's milk had significantly (p<0.05) of (protein, fat, solids not - fat and ash) in comparison to cow's and goat's milk (7.535, 6.617, 12.026 and 0.646) % respectively. Cow's milk had significantly (p<0.05) higher lactose content (4.757%) than both sheep's and goat's milk. components of milk 2. PhysicalThe results revealed that there were significant differences (p <0.05) in all physical characteristics of milk among different species of animals involved in this study (cows, sheep and goats). Sheep milk had significantly (p<0.05) (Titratable Acidity 0.182% and conductivity 3.214 mS/cm) than both cow's and goat's milk , goat's milk contains the highest specific gravity 1.057mg /100 ml while the pH values of cow's , sheep's and goat's milk were similar. Heavy metals in milk - Effect of Species The results revealed that significant differences (P<0.05) among the mean values of heavy metal concentrations included in the study (Cadmium (Cd) , lead (Pb), Nikel (Ni), Arsenic (As) and Mercury (Hg) in milk of different species (cows , sheep and goats). Sheep's milk had significantly levels of such heavy metals (0.251, 0.801, 0.563, 0.283 and 0.124) mg/ l respectively than both cow's and goat's milk while cow's milk had significantly the lower levels (0.098, 0.311, 0.221, and 0.122 , 0.037) mg / l respectively. - Effect of regions : The results showed that significant differences (P<0.05) in the means values of heavy metal concentrations in milk among different regions. The Highest levels of heavy metals were observed in AL - Hamza district (0.184, 0.624, 0.494, 0.246 and 0.119) mg / l respectively and the lowest levels of heavy metals were noticed in Dagharah (0.144 , 0.507, 0.382 , 0.167 and 0.061) mg / l respectively. - Effect of month : The results of the statistical analysis showed that there were non - significant differences in the average concentrations of cadmium, lead and arsenic among months while a significant differences (P <0.05) were observed in the level of nikel between march and other studied months [(0.494) mg / l in march and (0.397, 0.418 and 0.415) mg / l for December, January and February respectively]. Also the results showed that there were a significant differences (P<0.05) in the levels of mercury between march and each of January and February [(0.106) mg / l in march and (0.081 and 0.083) mg / l in January and February respectively]. From the results obtained in the present study,it can be concluded that : 1 - Heavy metals like cadmium (Cd), lead (Pb), Nikel (Ni), Arsenic (As and Mercury (Hg) which distributed among different sites were exceed the maximum acceptable limits set by WHO and EPA. 2 - High concentration of heavy metals (Cd, Pb, Ni, As and Hg) in city center site may be due to the industrial activities. 3 - High concentration of heavy metals in the other sites may be due to application of waste water for agriculture purposes (irrigation) which pollute the soil and also be allowing toxic metals to enter in to the food chain posing a serious health hazard.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الصحة العامة
السنة: 2014
الموضوع العام: الطب البيطري
اسم المشرف: كريم ناصر طاهر
اسم المؤلف: علياء حسن علـي السماوي

دراسة شكلية ونسجية وكيمياء نسجية للمعدة في الجاموس المحلي البالغ Bubalus bubalis Histomorphological And Histochemical Study of Stomach of The Adult Local Buffalo Bubalus Bubali

المستخلص: اجريت هذه الدراسة على عشرين معدة للجاموس المحلي الخالية من الامراض والتي جمعتمن مجزرة العـــشار في البصرة. تضمنت الدراسة الشكلية قياسات متعددة للاجزاء الاربعة من المعدة كالطول والارتفاع علاوة على حساب معدل عدد الحليمات الموجودة في الكرش في السنتمتر ال | 20 Stomach of healthy stomach of local buffalo were used in present study which collected from Al. Ashar Massacre in the city of Basrah.morphological study included several measurements for all parts of Stomach like length and width in addition to calculate the average of number of papillae that present in rumen per each Square centimeter and the average of length of these papillae as well as find the diameters of openings between chambers of Stomach. While the histological part was comprise investigate the histological structure of the wall of chambers of Stomach. Histological Section were prepared from these chambers stained with hematoxylin and eosin Stain to Study the general characters of tissue, while other sections were Stain with special Stains to detect the histochemical properties of these tissues. The morphological study showed that the stomach of buffalo formed from four chambers these are rumen, reticulum , omasum and abomasum differs in their length and width. The inner lining of rumen was contained on many papillae the average of length was 800 micrometers while their average of number was 118 papilla /cm2. while the inner lining of reticulam was included several number of lamellae which arranged in form like honeycomb. Omasal lining showed crescent lamella differs in their number and length while the wall of abomasums is divided in to two regions real gastro glands origin region and the color red and has a helical folds the seconed region is the pyloric glands region, a light colored region and the container on the irregular folds. In Histological part the present study revealed that the wall of all the chamber in buffalos, stomach were histologically composed from four layers; these are tunica Mucosa , Submucosa , Muscularis and Serosa.The tunica Mucosa of rumen was characterized by of papillae that differs in their number and length according to its region in rumen while the glands were abscent in lamina properia as well as abscent of muscularis mucosa, while the wall of abomasums appeared divided histologically into 3 regions ; cardiac , Fundic and pyloric region About the histochemical part , this study demonstrated that the collagen fibers were prevalent more density in Submucosal tunica than other histological tunicas in wall of rumen ,in contrast the elastic fibers were highly constricted in muscular tunica of the rumen and mucosal larger of reticulum in comparion with other tunicas and Submucosa of the Ommasum ,the elastic fibers showed prevalenced in all layers of the cardic region of the abomasums. this study showed prevalence of keratin in the mucosa denser than other layers in the rumen , reticulum and omasum while abscent in abomasum. this study also showed prevalence of glycogen and proteins in all parts of rumen as well as prevalence of fat and alkaline phosphatase activity in the wall of all chambers of stomach.
الصفحات الاولى:
مكان الجامعة: البصرة
اللغة: العربية
الجامعة: جامعة البصرة
الدرجة: ماجستير
الموضوع الدقيق: التشريح والانسجة والاجنة
السنة: 2015
الموضوع العام: الطب البيطري
اسم المشرف: فوزي صدام محسن الاسدي
اسم المؤلف: سوسن غفوري احمد

دراسة تشخيصية وامراضية نسجية للقناة الهضمية في الدجاج المنزلي Gallus gallus domasticus المصابة بالطفيليات المعوية في مدينة البصرة Diagnostic And Histopathological Study of Intestinal Tract of Domestic Fowl Gallus Gallus Domesticus Infected With Intestinal Parasites At Basrah City

المستخلص: امتدت الدراسة الحالية من شهر تشرين الاول 2011 ولغاية شهر اذار 2012 , حيث جمعت خلالها 108 نموذجا بواقع 36 عينة من الذكور و72 عينة من الاناث من الدجاج المحلي1758) Gallus gallus domesticus (Linnaeus, من محافظة البصرة بهدف عزل الديدان المتطفلة على القناة الهض | The recent study extended from October 2011 to January 2012, collected 108 samples by 36 samples of males and 72 samples of female of local chickens Gallus gallus domesticus (Linnaeus, 1758)from Basrah to isolate parasitic worms on the gastrointestinal tract and diagnosed as well as study percentage and severity of the infection, as well as monitoring biological activities of isolated parasites and find out macroscopic and pathological changes caused by these worms and find out the impact of tapeworms on the values of the blood parameters of chicken. The results showed infected of chicken with five types of tapeworms (Hymenolepis carioca, Raillietina tetragon, Raillietina cesticillus, Choanotania infundibulum, Cotugina sp) with percent of infection and severity reached 4.62%, 11.4; 19.44%, 12.09; 5.55%, 12.66; 9.25%, 12.6; 6.48 %, 11.14 respectively and got one type of nematodes Subulura brumpti with percentage of infection and severity reached 10.18%, 91 and three types of trematode Echinostoma revulatum, Hypoderm coniodium, Psilolecithum longorchum with percent of infection and severity reached 1.85%, 15.5; 0.92%, 10; 0.92% , 7 respectively.The results of the recent study showed that infection with two types is the most common reaching incidence of 25.92%, and study results indicated that there were significant differences between males and females in susceptibility to intestinal worms.Observed by studying the sites of worms in the gut that the proportion of tapeworms in the small intestine are more than in the large intestine while recorded nematodes only in the large intestine As for worms Trematode has found in the small intestine. The results also included monitoring biological activities of isolated parasites from the gastrointestinal tract from the beginning of the division of the egg and develop into a larva has been observed that the nematodes have the ability to survive outside the host, who were present more than tapeworms.the study contained a physiologic side on the impact of tapeworms on some blood parameters where showed infection of domestic fowl with anemia by observing the decrease in the number of red blood cells R.BC , hemoglobin Hb and P. CV while there was an increase in the number of white blood cells WBC. The study recorded the number of grossly and histological lesions in the intestine and the cecum of domestic chicken Because they infected with tapeworms, nematodes and flukes. grossly lesions included blockage and swelling in the intestines infected with tapeworm and cecum infected with nematode and appearance of rough nodules on the walls of the intestine infected with tapeworm with hemorrhage and the appearance of yellowish green purulent material in the inner wall of the intestines, and the histological changes has included atrophy of intestinal villi with sloughing and desquamation of epithelial layer with appearance of inflammation in the form of pseudomembrane and infiltration of inflammatory cells and coaglative necrosis, it have seen hemorrhage and superficial erosion in the mucous layer and sloughing of villi and epithelial layer with hyperplasia appearance purulent cells and destruction of intestinal glands and ulceration in the mucous layer in addition to the congestion
الصفحات الاولى:
مكان الجامعة: البصرة
اللغة: العربية
الدرجة: ماجستير
السنة: 2013
الموضوع العام: الطب البيطري
اسم المشرف: غازي يعقوب عزال الامارة
اسم المؤلف: اسراء صبيح بداي الغنامي

استخــدام فحصي الاليـزا واثباط التلازن الدموي في تحديد معيار الاضداد في افراخ اللحم المحصنة بطرق مختلفة ضد مرض النيوكاسل Using of Hi And Elisa For Detection of Antibody Titers Against Newcastle Disease Vaccines Using Different Methods of Vaccination In Broiler Chicks

المستخلص: استخدم في الدراسة 200 فرخ لحم سلالة روز بعمر يوم واحد وغير مجنس لغرض استخدام فحصي الاليزا واثباط التلازن الدموي في تحديد معيار الاضداد في افراخ اللحم المحصنة بطرق مختلفة ضد مرض النيوكاسل واجريت الدراسة في فرع الامراض وامراض الدواجن / كلية الطب البيطري / | In this study 200 Ross breed broiler chicks aged 1 day and in both sex used for using of ELISA and HI tests in determination the titer of antibody in broiler chicks which vaccinated by different ways against Newcastle disease. the study done in a special house in college of Veterinary Medicine / Basra University / Department of Pathology and Poultry disease. The chicks divided randomly to 5 treated groups each one contain 35 chicks and the 6th group not treated (Act as control) contain 25 chicks. All chicks provide free feed and the 5 groups vaccinated by different ways against Newcastle disease , in 7th day vaccinated with Hitchner B1 vaccine and in days ( 21 and 35) with LaSota vaccine.The 1st and 2nd groups vaccinated by drinking water , in the 1st group used of tap water mixed with Skimmed milk while in the 2nd group used of RO Water. The third group vaccinated by spray , the 4th group vaccinated with eyes drop and the 5th group vaccinated in nostril. The distilled water used with vaccine of third , 4th and 5th groups , the control group leaved without vaccination. All groups vaccinated against Gumboro disease in 14th day of age. The blood sample collected in ages of ( 1 , 21 , 35 , 49 ) days with the range of 10 specimens from each group and 5 specimens from the control group then serum separated in order to testing immunity by using of Elisa and HI tests.Because of maternal immunity the titer of antibody in the 1st day was very high in range different in probability (0.05 >P) in comparison with other days (21 , 35 , 49 ) in other groups.The result of ELISA test show that the 1st group was excellence in (49) days of age that used the tap water mixed with skimmed milk in comparison with other groups in days ( 21, 35 ) with range different in probability of (0.05 >P). The 2nd group which was used of RO water the second order while the third group which used the spray vaccination was in third order and the 4th and 5th groups were some that the same in result and there was no any moral different.The result of HI test show that the 1st group that used of tap water mixed with skimmed milk was excellent in (49) days of age in comparison with other groups in days ( 21 , 35 ) with range moral different in probability of (0.05 >P). The 4th group that used of eye drop vaccination was in second order , the third group that used of spray vaccination in third order while the 2nd and 5th groups were somewhat similar in result and there was no moral different.We concluded that the vaccination by using of drinking water that consist of tap water mixed with Skimmed milk was the best methods and gave an important satisfactory results in comparison with other methods. The ELISA test have high efficiency and best than HI test in detection titers of Newcastle disease antibodies due to the test sensitivity in measurement of IgY present in vaccinated chicks serum while the HI test didn’t reach the same sensitivity of ELISA test in complete detection of this immuno globulin ( IgY ).
الصفحات الاولى:
مكان الجامعة: البصرة
اللغة: العربية
الجامعة: جامعة البصرة
الدرجة: ماجستير
الموضوع الدقيق: الامراض - الدواجن
السنة: 2010
الموضوع العام: الطب البيطري
اسم المشرف: علي عبد سهم المياح
اسم المؤلف: محمد حميد خلف

دراسة مصلية لداء المقوسات في الجمال Serolgical Study of Toxoplasmosis In Camels

المستخلص: شملت الدراسة فحص مصول 334 جمل جمعت خلال الفترة من الاول من تموز - ولغاية نهاية شهر ايلول لعام 2010 متوزعة على 219 ذكر و115 انثى من الحيوانات الواردة الى مجزرة النجف الاشرف لغرض التحري عن الاجسام المضادة لطفيلي داء المقوسات باستخدام فحص تلازن اللاتكس Late | This study was conducted to examine serum of (334) Cameles in Al - Najaf slaughter house, which include (219) camel males and (115) camel females during the first of July until the end of September/ 2010 to investigate antibodies against Toxoplasmosis by Latex agglutination test as well as to estimate the levels of their antibodies by using semiquantative test.The total percentage of infestation 20.35%, and the sex has non significant on their infestation in examined camels (16.89%) in camel males and 26.95% in camel females.The highest percentage of seropositive with Toxoplasma is recorded in adult camels 23.33%, while it is (15.3%) in young camels.The results revealed that the highest titration of antibodies level was 32 (19.11%), and there is no significant effect of sex on antibodies level, while the age of examined camels was affect significantly.
الصفحات الاولى:
مكان الجامعة: البصرة
اللغة: العربية
الجامعة: جامعة البصرة
الدرجة: دبلوم عالي
الموضوع الدقيق: الطب الباطني والوقائي
السنة: 2010
الموضوع العام: الطب البيطري
اسم المشرف: قاسم حليم كشاش
اسم المؤلف: سعيد محمد حسن حيدر المظفر

عزل وتشخيص جرثومة ال (Pasteurella multocida) من الابقار والاغنام وتصنيف انماطها المصلية باستخدام تقنية تفاعـل السلسلة المتبلمرة Isolation And Diagnosis Pasteurella Multocida From Cattle And Sheep And Serotyping Classification Using Polymerase Chain Reaction Technique

المستخلص: تضمنت الدراسة جمع 150 نموذج من الرئات المصابة والمسحات الانفية ومسحات اللوزتين من الابقار والاغنام للمدة1 / 11 / 2010 ولغاية1 / /4 2011 من حظائر حيوانية ومجازر مختلفة في مدينة الديوانية. تم زرع العينات على ( وسط اكار الدم ووسط الماكونكي واكار تربتون الص | The study included the collection of (150) samples of infected lungs and nasal , tonsils swabs from cattle and sheep at the period 1/11/2010 to 1/ 4/2011 of farm animals and various massacres in the city of Diwaniya. Samples were cultured on the blood agar and MacConkey agar and Trypticase Soya agar then diagnosed after pure isolation of colonies of pasteurella multocida were diagnosied and identified using morphological and biochemical features. The results revealed that the total isolation percent of Pasteurella multocida was 82(54.6%) which distributed as 40(53%) and 42(56%) for cattle and sheep ,respectively.on the other hands ,the high percent of isolation were recoverd from infected lungs 16(64%) followed by tonsils 13(52%) then nasal swabs 11(44%) in respect with cattle ,while in sheep ,the percent were 17(68%) for infected lungs ,11(44%) for nasal swabs and 14(56%) for tonsils swabs The percent of P.multocida isolation in cattle was 28(51.8%) in age group (>2years)while it was 12(57.1%) in age group (<2years),where as these percents in sheep were 27(56.2%) and 15(55,5%) ,respectively. In male ,the percent of isolation were 22(45.8%) and 20(54%) in females in respect with sheep samples ,while it was 21(51.2%) and 9(55.81%) in cattle respectively ,there was no significant differences (p>0.05) between isolation percent according to species ,sex and age of animals. The results of polymerase chain reaction (PCR) as confirmatory test for P.multocida isolates after DNA extraction and amplification with specific primers named KMT - 1 showed asingle band amplified DNA with amolecular weight of 460 bp. In purpose of serotyping the isolates of Pasteurella multocida by using PCR, the specific primer of capsule ( CAPA, CAPB, CAPD, CAPE, CAPF) were amplified the results revealed that the serotype (B) was the dominat in cattle , with molecular weight (760pb) while type ( A) the dominat in sheep with molecular weight (1044pb). In Coclusion , the result of molecular diagnosis of P.multocida serotyping by using PCR revealed ahigh specificity (84.5 %) sensitivity (97.05%) in Comparision with routine diagnostic of this bacteria in cattle and sheep.
الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
السنة: 2012
الموضوع العام: الطب البيطري
اسم المشرف: عدنان حمد عبيد الحمداني قاسم حليم كشاش
اسم المؤلف: جنان ناظم صادق العبيدي

التحري عن الاصابة بفيروس جدري الابل في بعض محافظات العراق باستخدام تقنية تفاعل سلسة انزيم البلمرة PCR

الصفحات الاولى:
مكان الجامعة: القادسية
اللغة: العربية
الدرجة: ماجستير
الموضوع الدقيق: الامراض المشتركة
السنة: 2012
الموضوع العام: الطب البيطري
اسم المشرف: هدى عبد الهادي علي النصراوي
اسم المؤلف: محمود شاكر عبد الامير عيدان مرزوق