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استخــدام فحصي الاليـزا واثباط التلازن الدموي في تحديد معيار الاضداد في افراخ اللحم المحصنة بطرق مختلفة ضد مرض النيوكاسل == Using of Hi And Elisa For Detection of Antibody Titers Against Newcastle Disease Vaccines Using Different Methods of Vaccination In Broiler Chicks
Author name:
محمد حميد خلف
Supervisor name:
علي عبد سهم المياح
General topic:
Veterinary Medicine
Specific topic:
Diseases - Poultry
Degree:
Master
University:
University Of Basrah - College Of Veterinary Medicine - Department Of Diseases And Poultry Diseases
Language:
Arabic
University location:
Basrah
First pages:
21T509 - p.pdf
Abstract:
استخدم في الدراسة 200 فرخ لحم سلالة روز بعمر يوم واحد وغير مجنس لغرض استخدام فحصي الاليزا واثباط التلازن الدموي في تحديد معيار الاضداد في افراخ اللحم المحصنة بطرق مختلفة ضد مرض النيوكاسل واجريت الدراسة في فرع الامراض وامراض الدواجن / كلية الطب البيطري / | In this study 200 Ross breed broiler chicks aged 1 day and in both sex used for using of ELISA and HI tests in determination the titer of antibody in broiler chicks which vaccinated by different ways against Newcastle disease. the study done in a special house in college of Veterinary Medicine / Basra University / Department of Pathology and Poultry disease. The chicks divided randomly to 5 treated groups each one contain 35 chicks and the 6th group not treated (Act as control) contain 25 chicks. All chicks provide free feed and the 5 groups vaccinated by different ways against Newcastle disease , in 7th day vaccinated with Hitchner B1 vaccine and in days ( 21 and 35) with LaSota vaccine.The 1st and 2nd groups vaccinated by drinking water , in the 1st group used of tap water mixed with Skimmed milk while in the 2nd group used of RO Water. The third group vaccinated by spray , the 4th group vaccinated with eyes drop and the 5th group vaccinated in nostril. The distilled water used with vaccine of third , 4th and 5th groups , the control group leaved without vaccination. All groups vaccinated against Gumboro disease in 14th day of age. The blood sample collected in ages of ( 1 , 21 , 35 , 49 ) days with the range of 10 specimens from each group and 5 specimens from the control group then serum separated in order to testing immunity by using of Elisa and HI tests.Because of maternal immunity the titer of antibody in the 1st day was very high in range different in probability (0.05 >P) in comparison with other days (21 , 35 , 49 ) in other groups.The result of ELISA test show that the 1st group was excellence in (49) days of age that used the tap water mixed with skimmed milk in comparison with other groups in days ( 21, 35 ) with range different in probability of (0.05 >P). The 2nd group which was used of RO water the second order while the third group which used the spray vaccination was in third order and the 4th and 5th groups were some that the same in result and there was no any moral different.The result of HI test show that the 1st group that used of tap water mixed with skimmed milk was excellent in (49) days of age in comparison with other groups in days ( 21 , 35 ) with range moral different in probability of (0.05 >P). The 4th group that used of eye drop vaccination was in second order , the third group that used of spray vaccination in third order while the 2nd and 5th groups were somewhat similar in result and there was no moral different.We concluded that the vaccination by using of drinking water that consist of tap water mixed with Skimmed milk was the best methods and gave an important satisfactory results in comparison with other methods. The ELISA test have high efficiency and best than HI test in detection titers of Newcastle disease antibodies due to the test sensitivity in measurement of IgY present in vaccinated chicks serum while the HI test didn’t reach the same sensitivity of ELISA test in complete detection of this immuno globulin ( IgY ).
