التوصيف المظهري والجزيئي لبعض الفطريات الخيطيه والخمائر المعزولة من اخماج العين واختبار حساسيتها الدوائيه تجاه بعض المضادات الفطرية == Morphological And Molecular Identification of Some Filamentous Fungi And Yeast Isolated From Eye Infections And Theirs Susceptibilities Toward Some Antifungals
Author name:
يسر فاضل عبد الامير الاسدي
Supervisor name:
زيدان خليف عمران المعموري
General topic:
Biology
Specific topic:
Microbiology
Degree:
Master
University:
University of Babylon - College Of Science For Girls - Department Of Biology
Language:
Arabic
University location:
Babylon
First pages:
24T2667 - p.pdf
Abstract:
تمت هذه الدراسة في مختبر التقنيات الاحيائية التابع لكلية علوم البنات / جامعة بابل للفترة من اكتوبر 2012 الى اكتوبر 2013، حيث تم جمع 165 عينة سريرية من المرضى المراجعين لاستشارية العيون في مستشفى الحسين العام ومن بعض العيادات التخصصية لاطباء العيون في محاف | The study was performed in Biotechnical Laboratory in Science collage for women, Babylon University from Oct.2012 to Oct.2013. 165 clinical samples were collected from patients with eyes infections were admitted to AL - Hussein main hospital in Karbala province and private clinics of eye physician, after diagnosis of eye infection by specialize physician the clinical samples collected by using swab from Cornea, Conjunctiva, Lacrimal sac, eye allergic, contact lenses and from pull water eye after surgery, the samples included different sex & age groups.All clinical samples culturing on SDA and PDA, Yeast was identified base on their color reactions on Chromo agar Other biochemical test were performed.Antifungal sensitivity was performed against 8 antifungal (Nystatin, Econazol, Amphotericine B, Flucosytosine, Miconazole Metronidazole, Griseofulvin, Ketoconazol, Most fungi showed variable degrees in their susceptibilities to the antifungal agents. In this study we diagnostic (57) isolate from filamentous fungal species, Aspergillus spp. and Fusarium spp. showed highly frequent in ratio 20.1% and 9.6, In addition (37) yeast isolated, C.albicans and C.famata representative the highest frequent isolates.Molecular identification and genotyping, 8 isolates identified as C.albicans from 24 of selective Candida isolates were performed by specific pair primer (CABC) that give genotype with 515 base pair, genotype ITS1/ITS4 region show polymorphic PCR product help in diagnosis other (7) Candida species like C.famata, C.rugosa, C.glabrat, C.utili, C.saitoana, C.guilliermondii, C.inconpicua. The pair primer for amplified the transposable intron (CA - INT) was succeed in amplification of intron showed two genotypes : type A (450bp) and type B(850bp) and showed there was no C.dubliniensis isolates in our Candida isolate, This result was coincidence typing results between specific pair primer (CABC) and Microsatellite CAI for C.albicans.The amplified 18s region by using NS/EF3 and the result give genotype with molecular weight 1700bp.Also the study include using RFLP technique & the digested by using CfoI enzyme & the results for ITS PCR products of 24 isolates of Candida showed unique RFLP patterns.
