عزل وتشخيص جرثومة ال (Pasteurella multocida) من الابقار والاغنام وتصنيف انماطها المصلية باستخدام تقنية تفاعـل السلسلة المتبلمرة == Isolation And Diagnosis Pasteurella Multocida From Cattle And Sheep And Serotyping Classification Using Polymerase Chain Reaction Technique
Author name:
جنان ناظم صادق العبيدي
Supervisor name:
عدنان حمد عبيد الحمداني | قاسم حليم كشاش
General topic:
Veterinary Medicine
Specific topic:
Microbiology - Biotechnology
Degree:
Master
University:
University of Al-Qadisiyah - College Of Veterinary Medicine
Language:
Arabic
University location:
Qadisiyah
First pages:
21T501 - p.pdf
Abstract:
تضمنت الدراسة جمع 150 نموذج من الرئات المصابة والمسحات الانفية ومسحات اللوزتين من الابقار والاغنام للمدة1 / 11 / 2010 ولغاية1 / /4 2011 من حظائر حيوانية ومجازر مختلفة في مدينة الديوانية. تم زرع العينات على ( وسط اكار الدم ووسط الماكونكي واكار تربتون الص | The study included the collection of (150) samples of infected lungs and nasal , tonsils swabs from cattle and sheep at the period 1/11/2010 to 1/ 4/2011 of farm animals and various massacres in the city of Diwaniya. Samples were cultured on the blood agar and MacConkey agar and Trypticase Soya agar then diagnosed after pure isolation of colonies of pasteurella multocida were diagnosied and identified using morphological and biochemical features. The results revealed that the total isolation percent of Pasteurella multocida was 82(54.6%) which distributed as 40(53%) and 42(56%) for cattle and sheep ,respectively.on the other hands ,the high percent of isolation were recoverd from infected lungs 16(64%) followed by tonsils 13(52%) then nasal swabs 11(44%) in respect with cattle ,while in sheep ,the percent were 17(68%) for infected lungs ,11(44%) for nasal swabs and 14(56%) for tonsils swabs The percent of P.multocida isolation in cattle was 28(51.8%) in age group (>2years)while it was 12(57.1%) in age group (<2years),where as these percents in sheep were 27(56.2%) and 15(55,5%) ,respectively. In male ,the percent of isolation were 22(45.8%) and 20(54%) in females in respect with sheep samples ,while it was 21(51.2%) and 9(55.81%) in cattle respectively ,there was no significant differences (p>0.05) between isolation percent according to species ,sex and age of animals. The results of polymerase chain reaction (PCR) as confirmatory test for P.multocida isolates after DNA extraction and amplification with specific primers named KMT - 1 showed asingle band amplified DNA with amolecular weight of 460 bp. In purpose of serotyping the isolates of Pasteurella multocida by using PCR, the specific primer of capsule ( CAPA, CAPB, CAPD, CAPE, CAPF) were amplified the results revealed that the serotype (B) was the dominat in cattle , with molecular weight (760pb) while type ( A) the dominat in sheep with molecular weight (1044pb). In Coclusion , the result of molecular diagnosis of P.multocida serotyping by using PCR revealed ahigh specificity (84.5 %) sensitivity (97.05%) in Comparision with routine diagnostic of this bacteria in cattle and sheep.
