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تاثير المستخلص الكحولي للبروبولس على بعض الفعاليات الحيوية في الارانب المصابة بالداء السكري == Effect of Ethanolic Extract of Propolis On Some Biological Activities In Diabetic Rabbits (Oryctolagus Cuniculus)

Author name: دعاء عبد الزهرة دلي الفتلاوي
Supervisor name: عبد الهادي عباس الابراهيمي | سهاد حميد حسن
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: العكبر (صمغ النحل) Propolis هو نتاج نحل العسل الذي اكتسب شعبية في الطب البديل وذلك بسبب خصائصه الحيوية، وقد استخدم بشكل واسع في الاطعمة الصحية. ان الدراسات المتعلقة بتاثير العكبر العراقي قليلة. ولهذا السبب, فان الهدف من هذا البحث هو تحليل تاثير العكبر الع | Propolis is a honeybee product that has gained popularity in alternative medicine, due to its biological properties and it has been intensively used in health foods. Studies concerning the effect of Iraqi propolis are rare. Therefore, the goal of this work is to analyse the effect of Iraqi propolis on some hematological and biochemical parameters in alloxan - induced diabetic rabbits, as well as study the histological observations of the pancreas, liver and kidney. Female local rabbits (Oryctolagus cuniculus) were used for this study. The overall number of animals used was 30.They were randomly divided into five groups. Diabetes was induced in all rabbits, except normal control, by a single dose of alloxan (150 mg/kg, i.v.). Development of induced diabetes mellitus was confirmed on first week after alloxan administration by examining the fasting glucose level in the blood taken from marginal ear vein. Rabbits with glycaemia were treated with alcoholic extract of propolis for 23 days. Diabetic control group did not treat with propolis. The treated animals were subdivided into three groups according to the dose of propolis extract. Three oral concentrations of propolis extract were investigated (50, 100 and 200 mg/kg/day).The following parameters have been studied were changes in weights of body, liver and kidneys ; changes in the hematological values that include erythrocytes, hemoglobin, leukocytes and hematocrit also changes in the biochemical values which included glucose, total protein, triglycerides, total cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), blood urea, creatinine and Malondialdehyde (MDA), in addition histopathological study of pancreas, liver, and kidneys. The results indicate a significant decrease (P < 0.05) in the body weight of alloxan - induced diabetic rabbits in comparison with control group, while there were significant increases in the weights of liver and kidneys. The present study showed that alloxan induced significant decreases (P<0.05) in all primary blood indices; erythrocytes, hemoglobin, packed cell volume (hematocrit) and leukocytes. Also, serum biochemical changes showed significant increases (P<0.05) in glucose, total protein, triglycerides, total cholesterol, AST, ALT, blood urea, creatinine, and MDA comparison with control group.Histopathological changes in pancreas, liver and kidneys, observed microscopically, revealed degrees of damage in the tissues, while these organs of control groups exhibited a normal architecture. The treatment with alloxan resulted in several forms of histological alterations such as cytoplasmic vacuolation, degeneration, necrosis, cell hypertrophy, nuclear diploid, diffusion of inflammatory cells, dilatation in ducts, stagnation in secretory fluid. in addition, hemorrhage, dilatation and congestion in blood vessels and disorganization of histologic architecture, Generally, the gradual improvement in blood values was noticed with the increase in concentration alcoholic extract of propolis and return back the normal histological shape of pancreas, liver and kidneys. Propolis extract in rabbits had a potent antihyperglycemic effect, antioxidant activities, radical - scavenging capacities, tissue regeneration properties, and that may be due to the high biological activity and nutritive values contents in bee propolis. In conclusion, the results suggest that propolis could potentially contribute for the prevention and treatment of diabetes mellitus.

تقييم مستوى هرموني الجوع والسمنة لدى مرضى فرط ضغط الدم في محافظة النجف الاشرف == Assessment of Obestatin And Ghrelin Levels In Hypertensive Patients In Al - Najaf Al - Ashraf Governorate

Author name: زهراء محمد فاخر عطية النفاخ
Supervisor name: ارشد نوري غني الدجيلي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: تهدف هذه الدراسة الى تقييم مستوى الهرمونات المكتشفة حديثا هرمون الجوع ?هرمون السمنةونسبة هرمون الجوع الى هرمون السمنة في مرضى ضغط الدم لمعرفة العلاقة المحتملة بين مستوى هرمون جريلين, اوبستاتين مع عوامل مختلفة بما في ذلك الضغط الانقباضي, الضغط الانبساطي و| The present study aims to assess the newly discovered ghrelin, obestatin hormones levels and ghrelin to obestatin ratio in patients with hypertension disease and to find out a possible relationship between obestatin, ghrelin level with different criteria including, systolic blood pressure, diastolic blood pressure and body mass index (BMI) in patients with Hypertension disease and compared with healthy controls as a tool for monitoring and even possibly prediction or diagnosis of these diseases. 69patients are diagnosed with hypertension disease (33males and 36 females) and 20 control healthy subjects (10 males and 10 females) having no history of diabetes mellitus, renal failure and other disease enrolled in this study.The patients are selected from both sexes (males and females) their ages ranged between (40 - 70) years old. Patients with hypertension disease are recruited from AL - sader teaching city in AL - Najaf AL - Ashraf Governorate during the period from September to December 2013. Results of the present study in general revealed that there is high obestatin level in patients with hypertension diseases in a comparison with control group, low ghrelin level in patients with hypertension disease comparing with control group and ratio of ghrelin to obestatin ratio was decreased in patients undergo from hypertension when compared with control group.The result showed a positive correlation among obestatin, systolic pressure, diastolic pressure and body mass index (BMI) with negative correlation between gherlin, systolic pressure, diastolic pressure, and body mass index (BMI) and negative correlation between ghrelin to obestatin ratio with blood pressure. The present study concluded that ghrelin to obestatin ratio might play role in blood pressure regulation

دراسة العوامل الوراثية والخلوية والتوصيف الجزيئي لاصناف الحنطة المزروعة في محافظة النجف باستخدام مؤشرات التتابعات البسيطة المتكررة SSR == Studying The Genetic And Cellular Factors And Molecular Characterization of Wheat Varieties Cultivars In The Province of Najaf By Using Simple Sequences Repeat Ssr Markers

Author name: صبا عبد المطلب المدني
Supervisor name: ميثم عبد الهادي السوداني | سلوى جابر العوادي
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: ضمن البحث دراسة عشرة اصناف من الحنطة الناعمة (حنطة الخبز) Triticum aestivum L. من الاصناف العراقية والمدخلة الى البلد من قبل وزارة الزراعة العراقية والمزروعة في محافظة النجف وهي : الشام6 , ابوغريب, تموز2, تموز3, العراق, النور, اللطيفية, اباء 99, الرشيد, و| The study included studying ten cultivars of soft wheat (wheat bread) of several types the Iraqi, foreign and imported types into the country by the Iraqi Ministry of Agriculture and cultivated in the province of Najaf, they are : Sham 4, Abu Ghraib, Tamoz 2, Tamoz 3, Iraq, Alnoor, Latifiya, Ebaa 99, Rasheed and Alforat to study the genetic stability using cytological studies. Cultivars were grown in Petri dishes and pots for the cytological studies that included studying the number of chromosomes in the somatic cells at the root tips and the chromosomal behavior during meiosis of the mega spores of the Pollen Mother Cells (PMCs). The cytological studies regarding the number of somatic chromosomes in the meristematic cells of the root tip revealed that they were in genetic stability regarding the level of chromosomal number and in a state of Eupliod in all the investigated cultivars. They also showed that the chromosomal behaviors during meiosis of the pollen mother cell (PMCs) was bivalent representing organized chromosomal behavior in the investigated cells which is regarded as an indicator and a proof of genetic stability of the studied cultivars. During the study, the genetic cultivars, using 15 primers of simple sequence repeat (SSR), gave all polymorphic between the cultivars and produced polymorphic 39 alleles at average of 2.6 for each locus, showed the genetic adendrogram of ten cultivars was performed by two groups in which the percentage of similarity between them was 75% as the results showed that the closest cultivars to each other were Tamoz 2 and Ebaa 99 which is the same genetic dimension between Tamoz 3 and Ebaa 99 reached 0.107 and by the similarity 89%, while the two cultivars alsham4 and Al - Rasheed were divergent (0.333) with 67% similarity.

تاثير عقار البريدنيزولون في بعض مناسيب الدم الهرمونية والكيموحيوية لدى مرضى الربو القصبي من الرجال في محافظة النجف الاشرف == Effect of Prednisolone Medicine On Some Hormonal And Biochemical Levels In Bronchial Asthma Patients of Men In Al - Najaf Governorate

Author name: نور زهير جاسم
Supervisor name: ارشد نوري غني الدجيلي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية لمعرفة تاثير عقار البريدنيزولون في بعض المناسيب الهرمونية والكيموحيوية لدى مرضى الربو القصبي من الرجال, اذ اجريت هذه الدراسة في مستشفى الصدر التعليمي في محافظة النجف الاشرف \ مركز الحساسية والربو للمدة من 1\7\2011 الى 1\1\2012. تم فحص | The present study was concerned to investigate the effect of prednisolone medicine in some of hormonal and biochemical levels in patients with bronchial asthma of men. The study was carried out in Sadr Teaching Hospital in Najaf City \ Center of Allergy and Asthma for the period from 1 \ 7 \ 2011 and up to 1 \ 1 \ 2012, (56) blood samples from men with bronchial asthma and who treated by prednisolone were tested. As well as a Healthy group consisting of (24) healthy persons who do not suffer from any health problems, the effect of medicine was tested on study levels. The statistical analysis of the results revealed that there was a significant increase (P<0.05) in the concentration of Triiodothyronine Hormone (T?) in patients with bronchial asthma of men who treated by prednisolone compared with Healthy group. The statistical results also showed a significant decrease (P<0.05) in the concentration levels of Thyroid Stimulating Hormone (TSH), Follicle - Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Testosterone Hormone in asthmatic group compared with Healthy group. The results of the biochemical investigations revealed a significant increase (P<0.05) in the concentration of Total Cholesterol, Triglycerides, Low Density Lipoprotein (LDL) and liver enzymes Alkaline Phosphatase ALP, Alanine & Aspartate Transaminase (ALT, AST), and lack of significant difference in the concentration of High - Density Lipoproteins (HDL) in the asthmatic group compared with Healthy group. The results of the current study also exhibited a significant increase (P<0.05) in the concentration of T? for the age group (21 - 30) years of the patient group, whereas the same asthmatic group recorded a significant decrease (P<0.05) in the concentration of FSH, LH and Testosterone Hormone in compared with Healthy group. The results of the study revealed a significant increase (P<.05) in the concentration of Total Cholesterol, Triglycerides, LDL and alkaline phosphatase ALP for the age group (41 - 50) years in asthmatic patients compared with Healthy group. The results of the current study also showed that the concentration of the TSH, FSH, LH and Testosterone Hormone had declined significantly (P<0.05) in asthmatic group who treated by prednisolone for (16 - 20) years compared with Healthy group. The concentration of Total Cholesterol, Triglycerides and liver enzymes Alkaline Phosphatase, Alanine Transaminase and Aspartate Transaminase (ALP, ALT and AST) had risen significantly (P<0.05) in asthmatic group who treated by the prednisolone for (16 - 20) years compared with Healthy group. The current study concluded that Treatment with prednisolone has hormonal and biochemical effects on bronchial asthmatic patients in all ages

دراسة بيئية وتصنيفية للقواقع المائية في مدينة النجف الاشرف وبعض المؤشرات حول حكة السباحين == Taxonomic And Ecological Study of Aquatic Snails In The Al - Najaf Al - Ashraf City And Some Pointers About Swimmer’s Itch

Author name: اسراء عبيد حسين الشبلي
Supervisor name: جاسم حميد رحمة الخزاعي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية في محافظة النجف الاشرف تم جمع عينات القوقع من ثلاث محطات ( (منخفض بحر النجف وقضاء المشخاب والعباسية)) للفترة من 1 - 2 - 2013 لغاية 30 - 10 - 2013 وفحصت العينات في مختبر اللافقريات التابع لكلية التربية للبنات لغرض تشخيص الاطوار اليرقي | This study was conducted in Al - Najaf Al - Ashraf province and the samples were collected from Bahar Al - Najaf, Al - Mashkhab and Al - Abbasia, during the period 1/2/2013 untill 30/10/. The samples were in the laboratory of invertebrates in College of Education for Girls to diagnos the larva stages of Trematodes which parasitize on the aquatic snails with some of the indicators and observations about the causes of Swimmer’s itch disease. The lowest water temperature in AL - Abbasia water was 13?c in February while the highest temberature was recorded in Bahar Al - Najaf 30.4?c in August. The PH ranged between (7 - 8.5).The Electrical Conductivity recorded the lowest rate in February in Al - Abbasia 1.16 Microsimnez / cm and the highest rate in March in Bahar Al - Najaf 5.97 Microsimnez / cm and the salinity concentration was lowest the in February in Al - Abbasia 0.58 ppt, while the highest rate was recorded in March in the Bahar Al - Najaf (2.98) ppt and Turbidity was the lowest rate in Al - Mishkhab in October 2.8 Unit Turbidity nephlometric and the highest rate was in the AL - Abbasia in July (4.42) units Turbidity nephlometric while The Dissolved Oxygen recorded the lowest level in the Al - Abbasia 2 mg / L in August and the highest level in April in Al - Mishkhab 8.5 mg / L. The study showed the presence of five types of snails : Belamia bengalensis, Lymnae auricularia, Physa acuta, Melanopsis nodosa and Melanoides tuberculata. therefore, there are five species of snails in Bahar Al - Najaf while P.acuta was not recorded in Al - Abbasia and Al - Mashkhab in addition, the numbers of the snails varied according to the time, so the B.bengalensis and M.nodosa increased in June, July and August while L.auricularia and P.acuta and M.tuberculata snails increased during spring season. All snails that have been diagnosed were infected with (Miracidium, Sporocyst, Ridia and five types of Cercaria) stages.Also, the highest infection in the M.tuberculata was 36.3%, B.bengalensis 31.3%, M.nodosa 25.4%, L.auricularia 24 % while the snail P.acuta recorded lower infection rate at13.7%. The Results of the questionnaire revaled that the males infection in Swimmer’s itch was 76% higher than females.The highest infection was among (10 - 20 years) it reached 36%, and 39% in fishmen and aquatic birds which is the highest rate compared with others. The highest rate of infection was recorded in August (42 %) a large number of the patients exhibited symptoms of the disease after coming out of the water and dehydration reached 52%.The results showed that 75% of patients showed Symptoms of the Swimmer’s itch after (3 - 7days), also the hands and legs were more susceptible to infection 62 %, and the highest percentage of the S.dermatitis 86% wdetected in person swimming in Shallow water

دراسة وبائيــة وتشخيصيـة للانماط الوراثية لطفيلي Giardia lamblia المسبب للاسهال لدى المرضى في محافـــظة النجــــف الاشرف باستخدام تقنيـــة الـ PCR == Epidemiological And Diagnostic Study of The Giardia Lamblia Parasite Genotypes, Which Causes Diarrhea Among The Patients In Al - Najaf Al - Asharf, Province, By Using The Pcr Technique

Author name: وداد هاشم يحيـى ناصر المحنة
Supervisor name: جميل جري يوسف الحميداوي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية في قسم علوم الحياة - كلية التربية للبنات - جامعة الكوفة للمدة من تموز 2011 ولغاية حزيران 2012 والتي تهدف الى دراسة وبائية طفيلي Giardia lamblia وتحديد الانماط الوراثية للطفيلي لدى المرضى المصابين بالاسهال في محافظة النجف الاشرف باستع | The Present study was carried out in the Department of Biology - College of Education for Girls - University of Kufa for the period from July 2011 until June 2012, it aims to study the prevalence of the Giardia lamblia parasite and identify genotypes of the parasite in patients with diarrhea in the province of Najaf by using the microscopic examination and the polymerase chain reaction (PCR).The results of microscopic examination of 3383 stool samples, 500 samples are infected by the G. lamblia parasite, with a total percentage of 14.8%. The results of the statistical analysis showed that there were significant differences at the level of probability P ? 0.05 where the rate of infection in the liquid samples was higher than it is in semi - liquid samples, reaching 17.2% and 10.7% respectively. As the results show the incidence of the parasite in males are higher than it in females reached 16.8% and 11.1%, respectively, as well as the spread of infection with the parasite in the rural areas more than the urban area where the ratio was 19.6% and 9.1%, respectively, the results also show that the highest infection rate with the parasite was in the age group aged (1ess than year) and the lowest in the group aged (36 - 40) years, reaching 28.0% and 10.0%, respectively. The results also show that the highest percentage of parasite infection was in the September, 44%, while the lowest infection rate was in December, reaching 6.1%. The results of the current study show that the highest infection rate parasite was in patients in the hospital of Manathira which amounted to 16.5% and that the lower infection rate was among patients of the Medical Al - Sader city hospital, reaching 11.7%. The results of extracting (100) Stool Samples by microscopic examination for patients infected with G.lamblia showed the presence of the gentic material DNA of the G. lamblia parasite in (41) samples which constitute 41%, and results of the examination by PCR by using Triose phosphate isomerase (Tpi) Showed that there are signiinficant differences at the level of probability, p ? 0.05 percentage infection with the genotype (B) compared with the genotype (A), which were 61% and 39.1% respectively.The highest rate of infection with the genotype (B) in the semi - liquid samples was 69.3% compared to the liquid samples, which amounted to 67.9%, while the genotype (A) had the highest rate of infection in the liquid samples, which amounted to 32.2% while it was 30.8% in the semi - liquid samples.As the results showed that the percentage of infected male with the genotype (B) was more than it is in females, reaching 62.5% and 58.9%, respectively, and in type (A) the infection of females was higher than the infection males 41.2% and 37.5% respectively. As the results show the spread of genotype (B) in the urban area is more than the rural area, the percentage of infection is, 72.8% and 66.7% respectively, while type (A) was more prevalent in rural than in the urban, where the percentage of infection is 33.4% and 27.3% respectively.The results also showed that the highest incidence of the genotype (B) was in the age group (41 - 45), it reached 75% and the lowest percentage in the age group(1 - 5) (11 - 15) (26 - 30) (36 - 40) years, amounting to 50%, while genotype (A), the highest rate of infection, when age group (1 - 5)(11 - 15)(26 - 30)(36 - 40) years to approximately 50% and the lowest percentage of infection in the age group (41 - 45), was 25%.

تاثير المستخلص الكحولي الخام لاوراق الجت (Medicago sativa) على بعض المعايير الفسلجية والكيموحيوية في اناث الجرذان المستحثه بمادة الالوكسان == Effects of Crude Alcoholic Extract of Alfalfa (Medicago Sativa) Leaves On Some Physiological And Biochemical Parameters In Alloxan Induced Diabetic Female Rats

Author name: حوراء سليم محمد طه بشيبش
Supervisor name: عبد الزهرة كاظم محمد علي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت هذه الدراسة في مختبر البحوث السريرية في كلية الصيدلة ومختبر فسلجة الحيوان في كلية العلوم / جامعة الكوفة للمدة من 15 / 9 /2011 الى 12 /3 /2012 للتقصي عن فعالية المستخلص الكحولي الخام لاوراق نبات الجت Medicago sativa L. في تخفيض مستوى سكر الدم وبعض ال | This study was conducted at the Clinical researches laboratory/ Pharmacy College and Animal physiology laboratory/ Sciences College / university of Kufa during the period from 15/ 9/ 2011 to 12 / 3/ 2012 to investigate the activity of the crud alcoholic extract of the alfalfa (Medicago sativa L). leaves in affecting the blood glucose level and some physiological and biochemical changes due to induced diabetes mellitus by alloxan (150) mg/kg in female albino rats (Ratus ratus), in comparison with the activity of the reference drugs Glibenclamide. This study was also used to know the effect of the extract in normal (healthy) female rats. In this study (54) female rats were utilized which were divided into (9) groups with 6 rats for each group. Diabetes mellitus was induced in (30) female rats, were distributed into 5 sub groups. One of them was considered as control group for the infected rats, and three groups of them were treated with different concentrations of the extract (250, 500 and 750)mg/kg while the fifth group was treated by the chemical drug Glibenclamide (0.6) mg/kg. The other 24 normal rats were also divided into 4 sub groups, one of them considered as control group. And the other three groups were treated with the extract concentrations (250, 500 and 750)mg/kg. The treating period with the plant extract and the drug continued for 30 day.Results of this study were as the following : ? A significant (P< 0.05) decrease was showen in total body weight, insulin hormone and HDL - c levels, total WBC and RBC count in addition to Hb concentration in infected group of diabetes mellitus in comparison with healthy control group.? A significant (P< 0.05) increase was clear in the levels of glucose, TC, TG, LDL - c, VLDL - c, urea, creatinine and ALT and AST enzymes in infected group in comparison with healthy control group.? The healthy rats that treated with the extract concentrations were showed a significant increase in their total body weight at the end period as compared with the first day of experiment, there was also a significant (P < 0.05) increase in body weights of the infected rats that were treated by the two concentrations (500, 750) mg/kg of extract as compared with infected control group.? The infected rats that treated by all three concentration of plant extract and the glibenclamid drug were exhibited a significant (P< 0.05) increase in insulin level as compared with infected control group.? on the other hand, it was also showen a significant (P< 0.05) decrease in glucose level of infected rats that have been treated by the three concentrations and the (0.6) mg/kg concentration of glibenclamid as compared with infected control group.? The levels of TC, TG, LDL - c, VLDL - c were significantly (P< 0.05) decreased, in contrast a significant increase was occurred in the level of HDL - c in infected rats when they treated by all the concentrations of plant extract as compared with infected control group.? The urea and creatinine levels showed a significant (P< 0.05) decrease when the infected rats were treated by the two concentrations (500, 750) mg/kg as compared with infected control group, while in the healthy rats, the urea level showed a significant (P< 0.05) decrease as compared with healthy control group.? A significant (P < 0.05) decrease was occurred in ALT enzyme level when the infected rats were treated by all extract concentrations and the chemical drug. But AST enzyme level was significantly (P < 0.05) decrease when the infected rats were only treated with the two concentrations (500, 750) mg/kg of plant extract in addition to glibenclamid as compared with healthy control group.? The two concentrations (500, 750) mg/kg of plant extract and the concentration (0.6) mg/kg of the glibenclamid drug caused a significant (P< 0.05) increase in total RBC count, and the hemoglobin concentration, while only the larger dose of plant extract caused a significant (P < 0.05) increase in total WBC count in infected rats as as compared with infected control group.In conclusion, the results of this study indicate that the two concentrations (500, 750) mg/kg of alfalfa leaves extract gave a good results, even better than the reference drug, glibenclamid for treating diabetes mellitus, in addition to ameliorate the metabolic disorders that accompany it.

تقييم مستوى بعض الحركيات الخلوية لدى المرضــى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1 == Evaluation of The Level of Some Cytokines In Patients With Herpes Simplex Virus Type I (HSV - 1)

Author name: حسين علي كاظم
Supervisor name: سهام جاسم الكعبي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية الى تسليط الضوء على مجموعة من المرضى المصابين بفايروس الحلا البسيط النمط الاول Herpes simplex virus - 1(HSV - 1) من خلال تقييم مستوى نوعين من الحركيات الخلوية الموجودة في مصولهم. شملت هذه الحركيات الخلوية الانترلوكين Interleukin - 6(I | The present study aimed to highlight a group of patients infected with herpes simplex virus type I (HSV - 1) by evaluating levels of two types cytokines in sera. These included cytokines interleukin - 6 (IL - 6) and tumor necrosis factor - alpha (TNF - ?) has been evaluated using the technique of Enzyme - linked immunosorbent Assay (ELISA), has also been investigating the relationship between these cytokines and the stages of infection compared to the control group. Before that rapid cytologic method has been used to diagnose the virus by detecting the presence of multinucleated giant cells, and then was diagnosed serologically by specific IgM and IgG monoclonal antibodies against Herpes simplex virus - 1. It was also used Radial immunodiffusion method to evaluate levels of total IgM and IgG in patients sera.Sixty four sera samples were collected from patients who are attending to the consultants clinics of Al - Hakeem general hospital and AL - Sader teaching hospital in AL - Najaf AL - Ashraf governorate for the period from October 21/2012 until March 13/2013. These Samples were divided into two groups : (32) sample during primary and recurrent infection, (32) after recovery from infection (latent stage), as well as (16) sample were collected from healthy individuals, who were regarded as acontrol groups.The study reached to the following results : • A significant increment (p<0.05) in sera concentrations of IL - 6 in patients during infection as compared with control groups, but the increment was not significant in sera of patients during latent stage.• TNF - ? levels increased in sera of patient, but this increment was not significant as compared with controls group.• There was no significant increase of Total IgM levels in sera of patients.• Total IgG levels increased significantly in sera of patients during infection and latent stage as compared with control group.• A highly significant positive correlation (r= 0.7, p= 0.00) between TNF - ? and IL - 6 concentration in sera of patients.• There was no significant correlation (p>0.05) between Total IgM levels and (IL - 6 and TNF - ?) in sera of patients with correlation coefficient (r= 0.1, r=0.2) respectively.• Positive significant correlation (r= 0.4, p= 0.02) was found between total IgG levels and IL - 6 in patients during infection, but was no correlation between them in latent stage.• There was no association between age and IL - 6 and TNF - ? levels in sera of patients with HSV - 1.Finally, the elevation of TNF - ? and IL - 6 levels in sera of infected patients with HSV - 1 may be play an important role in pathogenicity of virus, as well as it may limits the viral infection.Furthermore, there are positive correlation between levels of TNF - ? and IL - 6 in sera of patients

دراسة بعض التاثيرات المرضية للدودة الدبوسية Enterobius vermicularis لدى الاطفال في محافظة النجف == The Study of Some Pathologaical Effects of Pin Worm Enterobius Vermicularis Among The Children In Al - Najaf Province

Author name: ساهرة عايد عبد الصاحب الموسوي
Supervisor name: هيثم محمد حمادي العوادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية للمدة من شهر تشرين الثاني 2011 الى شهر تموز 2012, لمعرفة تاثير الدودة الدبوسية Enterobius vermicularis في بعض المعايير الدموية والكيموحيوية والسريرية لدى الاطفال المصابين بالدودة (12 - 4سنة). استخدمت طريقة الشريط اللاصق الاسكتلندي في | The present study was conducted during the period from November 2011 to July 2012, to evaluat the effect of the pin worm Enterobius vermicularis on the Hematological, biochemical and chlinical parameters in infected children (4 - 12 year).This study included 300 patient from both sexes and different ages. The Scotch Cellulose Tape Technique used for the examination of pin worm in children, attended Al - Sader Educational Hospital, Al - Zahraa hospitals and central laboratory in Al - Najaf Al - Ashraff city. The results of the sudy showed a significant decrease was noticed in Hb and PVC in infected person compared with the control group While there was a significant increase in each of the total number of white blood cells and Eosinophils and Neutrophile and a decrease in Lymphocyte, and it did not show any significant difference in the Basophilis and Monocyte in children infected with pin worm compared with the control group.The results of the study showed a significant increase in the levels of Nitric Oxide, Malondialdehyde, it also showed a significant decrease in the levels of Gluthanione in the infected group compared with the control group. The concentration of Copper, Zinc, Magnesium and Vitamine B12 were low in children infected with pin worm compared with the control group. The high incidence rate of infection accompined with patient suffering from pruritus ani and nocturnal enuresis. The age group (10 - 12) year represent the highest group infected with the worm.The education level influence the distribution of these worm among un educated families, and ratio of infection increase in male and female in families which contain more than (10) members with significant difference P<0.05 and this ratio decreased in children in families which contain between 3 - 4 members.This study did not show any significance defferent in infection among the ratio of infection between male and female under P<0.05.

عوامل الخطورة لطفرات الجينين CBS وMTHFR لقبل الارتعاج لدى النساء الحوامل في النجف == Risk Factors of CBS And MTHFR Gene Mutation For Preeclampsia In Pregnant Women In Al - Najaf

Author name: زهراء سامي رزاق النجار
Supervisor name: ظافرة جعفر عبد علي الفتلاوي | ماجد كاظم حسين
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: تعد الاصابة بقبل الارتعاجPre - eclampsia من الاعتلالات الخطيرة التي قد تظهر في الثلث الثالث من مدة الحمل اذ تتصف هذه الاصابة بارتفاع ضغط الدم وظهور بروتين الالبومين في الادرار. وقد ظلت اسباب نشوء هذه الحالة غير واضحة المعالم, اذ اعزيت بعض من اسبابها الى ا | Preeclampsia is considered as one of the serious complication in the third trimester of pregnancy. It is associated with hypertension and proteinuria. The causes of preeclamptic are still not well understood. Evidences suggested the involvement of metabolic related mutations in the disease. In the current study, the genetic mutations of CBS and MTHFR genes are studied as markers for the diagnosis of preeclampsia. Sixty preeclamptic pregnant women and forty healthy pregnant women as control group diagnosed by the physicians at Azzahra Teaching hospital for obstetrics &pediatrics in Najaf province from December 2012 to August 2013 The poly merase chain reactions technique was used to evaluate the incidence and frequency of C?S &MTHFR mutations. A questionnaire was conducted to obtain the information of the patients and the control group. Genetic mutation were detected by polymerase chain reaction (PCR)and agaros gel electrophoresis. The information indicated that the age of pregnant women, gestation age, previous history of the patients, family history and parity are involved in the pathogenesis of preeclampsia. The results of the present study of preeclampsia showed a significant increase (P ? 0.01) in patients (25 - 34) years old when compared with the other group. where the results found a significant increase (P <0.01) of Preeclampsia occurred during (33 - 39) weeks of pregnancy. And in the patients with previous history for preeclampsia there was significant increase (P<0.001) compared with healthy pregnants. Pregnants with preeclampsia in the family history showed significant increase (P< 0.01) as for women pregnant for the first time showed and significant (P<0.01) and constituted 8% pregnanst with preeclampsia who had diabetes mellitus. The extraction and quantification of cell free fetal DNA (cff DNA) revealed a significant (P<0.0001)increase in preeclamptic patients when compared with those of healthy pregnant women. It is confirmed by the use of an internal negative control through the monitoring of SRY gene (Y chromosome gene) amplification that the gene belong to the infant and 40 pregnant woman were followed up till the labor. Thus, 9 of them had delivered males, while 30 had delivered females. The wild type SRY gene band was obtained clearly from those of male delivery, but not from those of female delivery. Mutations of C?S gene were analyzed by theuse a primer intended to amplify a fragment of171 bp.The rate of mutation was found to be 51.7% and 5% in preeclamptic patients and healthy pregnant women respectively and Odd Ratio=20.31, P value=10 - 7. The C677T mutation of MTHFR gene was verified by the use of a primer designed to amplify a fragment of 198bp. The prevalence of mutation was indicated to be 68.3% and 17.5% in the preeclamptic patients and healthy pregnant women respectively and Odd Ratio =10.17, Pvalue =6.2*10 - 7. Results demonstrated the wide involvement of C?S and MTHFR gene mutation in the pathogenesis of preeclampsia, but they are no crucial for the development of the disease. In addition there are so many factors thatmay be involved in such development.

دراسة جزيئية للـ Bacteriocine المنتج من قبل بكتريا lactobacillus salivarius == Molecular Study of Bacteriocine Produce By Lactobacillus Salivarius

Author name: ايمان حمزة محمد
Supervisor name: احلام نعيم كاظم الياسين
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية التحري عن قابلية عزلات بكتريا Lactobacillus salivarius على انتاج البكتريوسين ودوره في تثبيط فعالية بعض العزلات البكتيرية السالبة لصبغة غرام. جمعت 155 عينة من مصادر سريرية مختلفة شملت 20عينة مسحات مهبلية، 8 عينة مسحات اللعاب، 6عينة حل | The aime of study detecting the a bility of Lactobacillus salivarius to produce bacteriocin and determine it’s role in inhibition the activity of some Gram negative bacteria. Atotal of 155 sample from different clinical source has been collected during the period from October - 2012 to March - 2013, these include 20 samples from vaginal smear, 8 samples from saliva and 6 samples from human milk.The results of primary isolation, Laboratory diagnosis and biochemical test showed that 34 isolates has bee identified as L.salivarius.Ahigh precent of isolation has been carried out from human milk which was 40%. The ability of all isolates to produce gelatinase and biofilm has been detected and the results revealed the ability of some isolates to produce gelatinase and biofilm. Ahigh percent of gelatinase production has been detected in L.salivarius isolated from milk sample (50%), while alow percent of gelatinase production was 0% in L.salivarius isolated from saliva. The results of optical density of biofilm produced by L.salivarius isolates from milk which was 100% higher than it in isolates from vaginal and saliva which was 85% and 75% respectively. All isolates shows antibacterial effect against some Gram negative bacteria, the diameter of inhibition as between (2 - 8), the results showed the ability of 4 selective isolates of L.salivarius to produce bacteriocin in which the Rf valve of precipitate bacteriocin was (0.42 - 0.6) compard with leaky bacteriocin hich was (0.57 - 0.8), also leaky bacteriocin as more activity against G - ve bacteria than precipitate bacteriocin. The result of plasmid profile in L.salivarius revealed the presence of megaplasmid in all isolate except one which had one megaplasmid and one small plasmid. Bacterial conjugation between L.salivarius that produce bacteriocin and had megaplasmid which used as adonor cell with E. coli Jm 109 as a recipient cell showed transferring of megaplasmid from donor to recipient cell and the transconjugant cells showed high anti - bacterial effect due to bacteriocin production against G - ve bacteria. The result of amplification of Sal P gene encoding salivracin P by PCR confirmed that all isolate had no Sal P gene in there genetic materials which revealed that these isolates had another type of bacteriocin rather than Salivracin P.

دراسة بعض الجينات المشفرة لعوامل الضراوة لبكتريا Acinetobacter baumannii المعرزلة من العينات السريرية == Study of Some Virulence Factors Encoded Genes of Acinetobacter Baumannii Isolated From Clinical Samples

Author name: عصام محمد طاهر الخويلدي
Supervisor name: مهدي حسين محيل العمار
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: اجريت الدراسة على300عينة سريريه مختلفة حصل عليها من الجروح والادرار والقشع والدم خلال الفترة من ايلول 2013 الى شباط 2014.منها 102(34%) لا يوجد نمو بينما 198(66%) نمت على وسط زرعي متخصص. شخصت عشرة عزلات من Acinetobacter baumannii من مائة وثمانية وتسعون من | Out of 300 various clinical samples obtained from wounds, urine, sputum, blood through the period from September, 2013 to February, 2014 of which 102 samples(34%) yielded no growth while 198 samples (66%) were positive growth on selective media. Ten isolates of Acinetobacter baumannii were identified among one hundred ninety eight of Gram - negative bacteria grown on MacConkey agar that was isolated from different clinical specimens in Al - Najaf Al - Ashraf province hospitals. A.baumannii identification depends on morphological, microscopic examination and biochemical tests as the initial identification. Also it was identified by both blood agar containing D - glucose and growth at 44?C. The final identification was performed by Api20E to confirm from all A.baumannii isolates..Distribution of A.baumannii among various clinical specimens where 4 isolates (40%) of wounds specimens and 3(30%) of urine specimens, followed by 2 isolates(20%) and one isolate(10%) of sputum and blood specimens respectively.The virulence factors of A.baumannii were studied, including bioflim formation, adhesion, capsule, hemolysin and protease, beta _lactamases production. The results indicate that 60% of A.baumannii formed the biofilm, 90% of isolates have ability to adhesion to epithelial cell and 70% of isolates was the capsule production, 80% of A.baumannii isolates able to produces beta _lactamases while all A.baumannii isolates cannot produce of both hemolysin and protease.The results showed variation in the resistance of these bacteria to antibiotics where all A.baumannii isolates 100% resistance to each of cefotaxime, Ceftazidime, ceftriaxon, piperacillin while 90% resistance toticarcillin - clavulanic acid, 50% resistance to tobramycin and 30% to ciprofloxacin. 20% of all isolates showed resistance to each of amikacin, Doxicycline, imipenem, meropenem.By using Combing Disc Test(CDT) method to detect Extended Spectrum ? - lactamases(ESBLs) and metallo ? - lactamases (MBLs) production.Where 90% of A.baumannii showed ability to produce of ESBLs while only 20% showed ability to MBLs production.The genotypic method was used to detect csuE, ompA genes and also some of ? - lactamases genes such as blaTEM, blaSHV, blaNDM - 1 by using Polymerase Chain Reaction technique.The results showed that 60% of all A.baumannii isolates produce phenotypic biofilm and 30% carry csuE gene of all isolates while 50% when compared with isolates that show phenotypic formation of biofilm and the distribution of gene among all clinical specimens where 50 %, 33.3% and 25% in sputum, urine and wounds specimens respectively while not present in blood specimens. The results showed that 40% of all A.baumannii isolates carry ompA gene while 44.4% when compared with isolates that show phenotypic adhesion and the distribution of gene among all clinical specimens where 100%, 50 %, 33.3% and 25% in blood sputum, urine and wounds specimens respectively.The outcomes showed 70 % of A.baumannii isolates carry blaTEM gene and 40% of A.baumannii isolates carry blaSHV gene while none carry of blaNDM - 1gene. When compared with phenotypic results of ESBLs, MBLs.The results of blaTEM, blaSHV genes become 77.7% and 44.4% respectively.

تاثير مستخلص بذورالحبة السوداء في امراضية طفيلي المتورقة العملاقة Fasciola gigantica خارج وداخل الجسم الحي == Efficiency of Nigella Sativa Seed Extract In Fasciola Gigantica Parasite In Vivo And In Vitro

Author name: شيماء عبد الحسين محمد شلاش
Supervisor name: جاسم حميد رحمة الخزاعي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية خلال المدة من شهر تشرين الثاني 2012 ولغاية تشرين الاول 2013لتقييم فعالية المستخلص الكحولي لبذورالحبة السوداء Nigella sativaفي حيوية بيض وبالغات دودة الكبد العملاقة Fasciola gigantica خارج وداخل الجسم الحي. وبواقع ثلاث تراكيـز 20%, 4 | The present study was conducted during the period from November 2012 till October 2013 to evaluate the effectiveness of the alcoholic extract for Nigella sativa seeds on the vitality of eggs and adults liver giant worm Fasciola gigantica In vitro and In vivo in the infected domestic rabbits lepus lepus arabica. Alcoholic extract for Nigella sativa seeds used In vitro at three concentrations 20%, 40%, 60% for each eggs and adults of Fasciola gigantica and In vivo were three doses 200, 400, 600 mg/kg from body weight in the infected rabbits by this parasite. The result of the current study In vitro revealed that the alcoholic extract for Nigella sativa seeds at 60% has been great effects in reducing the percentage of the eggs hatching to 0% and increasing the mortality percentage of the adult worms to 100% when compared with the control groups. So the results of this study showed that there is not significant differences of alcoholic extract of Nigella sativa seeds on the blood components of the healthy rabbits at level P<0.05 while in the infected and treated rabbits there are less significant effect when compared with the infected rabbits. In the infected rabbits showed significant increased in numbers of white blood cells from 5.52*109/L in the negative control to 10.41*109/L in the positive control and significant decreased in numbers of red blood cell from 5.51*1012/L to 4.41*1012/L and haematocrit value from 32.21% to (24.71%) and the amount of hemoglobin from 11.43*gm/dl to 8.53* gm/dl.While the ethanol extract of Nigella sativa seeds reduced the number of WBCs and increased the number of RBCs, PCV and the concentration of hemoglobin. Also the results of the current study In vivo showed that the ability of alcoholic extract of Nigella sativa seeds at dose 600 mg/kg are more effective in reducing the numbers of Fasciola gigantica worms in the infected and treated rabbits when compared with the infected and untreated rabbits. So the total rate number of worms in the positive control 16.7 while 6.4, 2, 0 for doses 200, 400, 600 mg/kg from body weight respectively. Results of histological study in the experimental infected rabbits revealed that the alcoholic extract is very effective for reducing the histopathological changes in the liver, spleen and kidney which caused by the parasite. And the therapeutic efficiency of these extract in dose 600 mg/kg for organs Liver, Spleen, Kidney are 100%. Concluded from the results of this study that the alcoholic extract of Nigella sativa seeds used in treatment the infection of the F.gigantica worm because its therapeutic efficiency and not any side effects to this extract.

تاثير الانزيمين الكلوكواميليز والكلوكوز اوكسيديز في تثبيط بكتريا Streptococcus mutans المسببة لتسوس الاسنان == The Effect of Glucoamylase And Glucose Oxidase On Inhibition of Streptococcus Mutans Causes The Dental Caries

Author name: بيداء عبود حسن الجنابي
Supervisor name: زهرة محسن علي | محمد عبد الله جبر
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: تضمنت الدراسة استخلاص الكلوكواميليز والكلوكوز اوكسيديز من الفطرين Aspergillus niger وPenicillum notatum على التوالي وتنقية وتوصيف الانزيمين لغرض زيادة تركيز بيروكسيد الهيدروجين المحفز لانزيم اللاكتوبيروكسيد الموجود باللعاب على تكوين ايونات الهايبوثايوسيان | This study aims at producing glucoamylase and glucose oxidase from the Aspergillus niger and Penicillium notatum respectively , purifying and characterizing the enzymes to increase H2O2 concentration which induce lactoperoxidase in saliva to the formation of hypothiocyanite ion to inhibite Streptococcus mutans which causes the dental caries. For glucoamylase and glucose oxidase production, the results shown the the highest enzymes production were occurred using the synthetic medium, it gave high titer of glucoamylase and glucose oxidase activity (0.212, 0.605 U/ml) respectively, the optimum incubation period for glucoamylase was occurred after 96 h (0.125 U/ml), while the maximum activity of glucose oxidase was occurred after 72 h (0.662 U/ml), the optimum temperature for enzymes production was occurred in 30°C, (0.257, 0.690 U/ml) respectively, the maximum production of glucoamylase and glucose oxidase were occurred in the pH = 6 (0.174, 0.391 U/ml) respectively, the optimum nitrogen source for enzymes production was yeast extract and potassium nitrate (0.157, 0.571 U/ml) respectively, the optimum carbon source for glucoamylase and glucose oxidase production was starch and glucose (0.167, 0.459 U/ml) respectively. For glucoamylase and glucose oxidase purification, the results found the ammonium sulfate (80 %) was selected as the best ratio for precipitate of glucoamylase , the specific activity reached to (3.626 U/mg) , with a purification fold (1.241) and yield (0.38) % , while the (60 %) was selected as the best ratio for precipitate of glucose oxidase, the specific activity reached to (10.304 U/mg) , with a purification fold (1.219) and yield (0.34) % , two peaks of glucoamylase were appeared in the gel filtration the specific activity of the first form (4.195 U/mg) with purification fold (1.436), while the second (31.214 U/mg) with purification fold (10.689), while one peak of glucose oxidase was appeared in the gel filtration, the specific activity was reached (62.382 U/mg) with purification fold (7.385). For characterization of enzymes, the results reported the highest activity of glucoamylase (Aand B) occurred in pH = 8, 6.5 (0.253U/ml, 0.511 U/ml) respectively , while the maximum activity of glucose oxidase (1.289 U/ml) in pH = 5.5, the optimum temperature of glucoamylase (Aand B) activity (0.243U/ml, 0.703 U/ml) in 40, 30 °C respectively , while the optimum temperature of glucose oxidase activity (1.424 U/ml) in 45 °C, the optimum pH for glucoamylase (B) stability ranging between (5.5 - 6.5), while the optimum pH for glucose oxidase stability ranging between (5 - 6) , the optimum temperature for glucoamylase (B) stability ranging between (10 - 35) °C for 30 min, while the optimum temperature for glucose oxidase stability ranging between (25 - 35) °C for 15min, the molecular weights of glucoamylase (Aand B) approximately 52 & 66 kDa respectively using the electrophoresis technique, while approximately 68 kDa for glucoamylase (B) using the gel filtration technique, the molecular weight of glucose oxidase approximately 78 and 87 kDa electrophoresis electrophoresis and gel filtration respectively, the values of Km and Vmax of glucoamylase and glucose oxidase were (2.4 mM , 9.6 mM/min and 19.6 mM, 7.5 mM/min) respectively. The result of this study showed the Streptococcus mutans growth was killed in the using the first and the second concentration of glucoamylase and glucose oxidase (0.09, 0.009 and 0.3, 0.03 µl) respectively , while the growth was appered in third concentration of enzymes (0.0009, 0.003 µl) respectively. The NaF, ZnF2 , C 12H 7 Cl 3 FNaO2 , NaCl , NaHCO3 and KSCN with (0.5 and 1M ) leads to increase the enzymes activity , while Na3PO4 , SrCl2 , Fe2O3 and Ca(HCO3) 2 caused decreased the activity of enzymes when treated with (0.5 and1 M) from these solutions, the glucoamylase activity also decreased when it treated with (0.5 and 1M) from KNO3 solution but this solution increased the activity of glucose oxidase when it treated with (0.5 and 1 M) from this solution

تاثير الري بالماء المعالج مغناطيسيا في نمو وحاصل الحنطة Triticum aestivum L == Effect of Irrigation With Magnetic Treated Water On Growth And Yield of Wheat (Triticum Aestivum L.)

Author name: فاضل كاظم كريم الابراهيمي
Supervisor name: ثامر خضير مرزة
General topic: Biology
Specific topic: Plant - Physiology
Degree: Doctorate
Language: Arabic
University location: Najaf
First pages:
Abstract: نفذت التجربة في حقل تابع للمعهد الفني - الكوفة شمال غرب محافظة النجف لموسم النمو (2012 - 2013) بهدف معرفة تاثير نوعية مياه الري وشدة معالجتها مغناطيسيا لثلاثة اصناف من حنطة الخبز Triticum aestivum L. في مؤشرات النمو الخضري والزهري والكيميائي والانتاجي.ص | An experiment was conducted in a field belonging to the Technical Institute - Kufa north west of Najaf governorate during the season of the year (2012 - 2013) to determine the effect of irrigation water quality and intensity of magnetically treated to three cultivars of bread wheat (Triticum aestivum L.) on vegetative growth, flowering, chemical and productive indicators.The experiment was designed randomized complete blocks (RCBD) arrangement Split split - plots and three replicatesfor each treatment. Experiment included study three factors is the quality of irrigation water (river water or well water) - Main plot, wheat bread varieties (IPAA - 99, Rashid and Tamuze - 2) - Sub - plot and magnetic treatment to irrigation water four intensities is (0, 750, 1500 and 3000) Gauss - Sub - sub - plot. The Revised Least Significant Difference (RLSD) at 0.05 probability was used to compare treatment means, whenever treatments affects were evident.Results showed : 1 - Wheat plants irrigated with river water were superior in all parameters in forms of plant height, leaves number, leaf area, tillers number; productive and non - productive, root length, lateral root length of the plant, leaves content from total chlorophyll, dry weights for plant vegetative, phosphorus% in grains, grains weight of spike, spike number of plant and m2, weight of 1000 grain, plant yield of grains, m2 yield of grains, hectare yield of grains per hectare compare to plants irrigated with well water, which recorded the highest chemical content of grains from nitrogen, potassium, magnesium, protein and carbohydrates percentages. The percentage of flowering, spikes length, spikes grains number were superior in the beginning with the well water, were superior at the end with river water.2 - Magnetic treatment with irrigation water by 1500 gauss revealed that noticeable superior was obtained in most of plants vegetative and floral characteristics, while the chemical characteristics had increased with magnetic water treatment by 3000 gauss and yield characteristics treated with 750 gauss. The effect of magnetic water treatment on leaves content from total chlorophyll and magnesium percentage in grains was negative.3 - The majority of vegetative, chemical and productivity parameters was increased with Rashid plants cultivar compared to same parameters for Tamuze - 2 plants cultivar which superior than IPAA - 99 plants cultivar except number of leaves, tillers number and spike grains number which superior with IPAA - 99 plants cultivar.4 - The triple interaction between experiment all factors revealed a significant effect on magnetized river water at 1500 gauss in Rashid and Tamuz - 2 plants cultivars giving highest vegetative growth indicators average which increased on plant yield with low magnetic water treatment (750 gauss) to Rashid plants cultivars and medium strongly (1500 gauss) for Tamuz - 2 plants cultivars, while the characteristics of grains chemical content were superior with same plants cultivars but magnetized treatment well water at 3000 gauss.

تقييم فعالية المستخلص المائي والكحولي لنبات الصبار Aloevera في الاصابة التجريبية لطفيلي Eimeria tenella لدى فروج اللحم == Evaluationof Alcoholic Andwateryextracts Efficacyofaloe Veraon Experimental Infectedbroilerwitheimeriatenella

Author name: زهراء حاتم حميدي عبود الكعبي
Supervisor name: هيثم محمد حمادي العوادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية على (216) فرخ دجاج نوع Rose 308مصاب بطفيلي Emiria tenella خلال المدة من شهر نيسان 2013 الى شهر تشرين الاول 2013, وقد تم في الدراسة الحالية تحديد بعـــــــــض المؤشــــــــــــــرات الدمــــوية (الخلايا المتغايرة /الخلايا اللمفاوية HL | This study was conducted to (216) broilers chickens (Rose 308 Trade) infected parasite with Eimeria tenella performed form April 2013 till October 2013, has been in the current study to identify some indicators of blood(Heterophils/ Lymphocytes, White Blood Cell, Red Blood Cell, Packed Cell Volume, Hemoglobin) and biochemistry (Glucose, cholesterol and total protein) and productive performance (Weight increase, Food conservation, Cansumbtion and Body weight befor slugtery) , We note at the parasite has infected chicks were used three extracts of Aloe vera (alcoholic and hot water and cold water), dosage chicks were to demonstrate the effect of different concentrations of these extracts on the productivity and physiological traits mentioned above. Then the experiment was designed, which include the following : 1 - The first group (control group) included 36 chicks and was given food with no anti - coccidial. 2 - The second group include 36 chicks in duplicate was infected but did not treated.3 - The third group inclouded 36 chicks in duplicate was given extract of Aloe vera Alcoholic ethyl in adose of (0.5 - 1.5 - 2)gm per mm body weight.4 - The fourth group inclouded 36 chicks in duplicate was given extract of Aloe vera Cold water in adose of (0.5 - 1.5 - 2)gm per mm body weight.5 - The fifth group inclouded 36 chicks in duplicate was given extract of Aloe vera Hot water in adose of (0.5 - 1.5 - 2)gm per mm body weight.6 - The sixth group include 36 chicks in duplicate was given Vetacox(trade Mark)that had both Sulphadimidin 80 gm and Diverdin8 gm.Results showed the following : - 1 - The alcoholic extract of Aloe vera at concentration (0.5, 1.5, 2) g/ml after an infection in the fifth week revealed alittle effect on the H/L when compared with eighth week of infection. also this extract lead to increase, the rate of (PCV), (Hb) , and (RBC) while lead to decrease the rate of (WBC) after eighth week of infection at concentration (2)g/ml. Also this extract lead to increase the rate of cholesterol, total protein and the rate of weight increased in the eighth week, while the amount of feed intake and feed conversion showed alittle effect, as well as the body weight befors slaughter has astrong effect at concentration(2)g/ml in the eighth week of infection. 2 - The cold and hot aquatic extract of Aloe vera at concentration(2)g/ml lead to decrease the ratio of H/L and increase the rate of (PCV), (Hb) and (RBC) after the infection in the eighth week, also lead to decreas the rate of (WBC) and increase the rate of cholesterol, total protein, the rate of weight increased, the amount of feed intake and the body weight before slaughter at concentration (2)g/ml in the eighth week of infection. Also recorded abetter efficiency of feed conrersion at concentration (2)g/ml in the eighth week of infection.

دراسة بيئية للطحالب الهائمة والملتصقة على النباتات المائية في نهر العباسية/ وسط العراق == An Ecological Study of Phytoplankton And Epiphytic Algae On Aquatic Macrophyta In Al - Abbasiya River/Middle of Iraq

Author name: شيماء جبار هادي الجحيشي
Supervisor name: احمد عبيس مطر | جاسم محمد سلمان
General topic: Biology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: اجريت الدراسة الحالية شهريا في اربعة مواقع على نهر العباسية وسط العراق للمدة من اذار 2012 الى شباط 2013، شملت الدراسة قياس بعض الصفات الفيزياوية والكيميائية لمياه النهر ودراسة الصفات النوعية والكمية لمجتمع الطحالب الهائمة (Phytoplankton) والطحالب الملتصقة | The present study was conducted monthly at four sites that located at Al - Abbasiya River middle of Iraq, for the period from March, 2012 to February, 2013 including studying some physiochemical properties of water and quantitative and qualitative of phytoplankton and epiphytic algae on aquatic macrophyta by selecting five species of aquatic plants : Phragmites australis Cav., Typha domengensis per., Ceratophyllum demersum L., Potomogeton pectinatus L. and Myrophyllum verticillatum L. Results also showed that, the air temperature ranged between 10.0 to 45.3 C?, while, the water temperature was 6.3 to 33.0 C?. Transparency values ranged between 0.29 to 3.06 m, while, the current velocity was 60 - 730 cm/ sec. The study also revealed that the pH was limited at the period of study ranged 6.97 to 9.13. Electrical conductivity and salinity were about 163.33 to 1157 ?s. cm and 3.68 to 23.0 0 ‰, respectively. Total dissolved solids values were about 277 mg/L to 900g/L. Total suspended solids values ranged between 0.09 - 37.0 mg/l. Results showed that, slightly alkaline at and bicarbonate ions prevailed, total alkalinity was 42 to 810 mg /L. Dissolved Oxygen values were 1.83 to 12.33 mg/L and biochemical oxygen demand was 1.4 to 8.0 mg/L, water hardness value in the studied sites ranged between 60 to 2000 mg CaCO3/L., While, calcium concentrations was 86.84 to 1783.0 mg CaCO3/L. and Magnesium concentrations was 6.70 to 268.66 mg CaCO3/L. Reactive phosphate concentrations in this study produced values ` ranged from 0.01 to 5.06 mg /L, and Nitrite concentrations ranged between nill to 5.20 mg /L, Nitrate concentration ranged between 0.09 to 6.47 mg /L. 199 species were recorded as total species number of phytoplankton at the period of study that, belong to Diatoms algae consisting of 61.30% : 88 species which belong to Pennales and 34 species for centrales, followed by Chlorophyta and Cyanophyta that were 42 and 21 species, respectively. Pyrrophyta 1, Euglenophyta 2, Rhodophyta 2, Pheophyta 5, Chrysophyta 5 species. Bacillariophyta gave the highest total number was 9303 × 104 to 1074 × 104 cell / L of fresh weight that was recorded at site 1 during April , 2012 and May, 2012, while, others algae 9823× 104cell/L in site 3 in July, 2012 compared to 1036 × 104cell/l at site 4 in April, 2012 for the total phytoplankton population density. Site 1 was the most divers sites with numerous number of species of algae, reaching the number of 66 genera and 85 species. while, the least number of species at site 3 were 65 genera and 70 species. April and October, 2012 have the most total cell numbers available for diatoms and non - diatoms algae. Nitzschia palea ; N.commnis and N. kutzingiana were the highest abundance of species showed an average of 46.80% of the overall phytoplankton sampled. Nitzschia was followed by Ankistrdesmus falcatus (25.53%) and Anabaena cylindrica (17.02%).There were some species present in all sites during the period from March, 2012 to February, 2013 i.e. Anabaena cylindrical, Dimorphococcus lanatus, Draparnaldia judayi, Euastrum dubium, Pilayella littoralis, Chrysoamoeba radiansklebs, Melosira distance, Stephanopyxis turris, Amphora ovalis, Cocconeis placentula, Gomphonema augur, G.tenuirostrum, Semiorbis sp and Pinularia biceps. Epiphytic algae types were diagnosed during the study of 256 species within 8 division. Bacillariophyta division was the dominate reaching 172 species composed to 67.18% of the total number, most of them belong to Pennales with 140 species that was more than Centrales 32 species, Chlorophyta 47 species form 18.35%, Cyanophyta 22 species form 8.59% and Chrysophyta and Pheophyta 5 species for both formed 1.95% , Euglenophyta and Rhodophyta 2 species for both formed 0.78% and Pyrrophyta algae that represented by one species and one genus. The highest genera number during the study period were Cymatopleura, Eunotia, Nitizschia, Cymbella and Gomphonema. Numbers of epiphytic algae varied in months and sites due to the host plants. The highest total number was 31039 ×104 cell/ L of fresh weight was recorded at site 1 during April, 2012 on leaves of Ceratophyllum demersum plant, while, the lowest total number was103×104cell/L of fresh weight which was recorded at site. 2 during June, 2012 on shoot of Potomogeton pectinatus. Many species of epiphytic algae were different among the study sites; the highest number was 148 species in Ceratophyllum demersum recorded 93 species on the shoot and 110 species on the leaves, while, the lowest number was 148 species on P. pectinatus recorded 36 on the root 36 and 52 species on the shoot and 60 species on the leaves. The result of study confirm that some of epiphytic algae were found at the most plant parts and different hosts, such as Anabaena cylindrical A. diolum, Lyngba aestuarii, Microcystis aeruginosa, Cosmacladium tubetculatum, Scenedesmus quadricauda, Palmodictyon, Mougeeotia, Pithophora oedogonia. Results showed that, some species favor some parts of host plant e.g Anabaena cylindrica was the most dominated on the Myrophyllum verticillatum and P.australis.Schizothrix tinctoria was dominated on the shoot of Phragmites australis while, algae Nostic linkia dominated on the shoot of Typha domengensis. Algae Ankistrodesmum falcatus, Pyramimonas tetrarhnchus ; Tetraspora cylindrical were dominated on the shoot of Potomogeton pectinatus. There were only seven diatom taxa common encountered on all plant part e.g. Cyclotella bodanicavar var. michiganensis on the shoot of Myrophyllum verticillatum. Fragilaraia capucina var. mesolepta, Cocconeis placentula and Achanthes affinis were dominated on the shoot of Phragmites australis, it was also found that, Navicula miniscula present on leaves of Potomogeton pectinatus and Ceratophyllum verticillatum. Algae N. notha and N. odioas were found on Myrophyllum verticillatum leaves. Ten species were recorded for the first time at this study in Iraq from phytoplankton and epiphytic algae from the period of March, 2012 to February, 2013 : one species of Cyanophyta epiphytic algae Anacystis nidulans ;one species of chlorophyta epiphytic Trentepohlia aurea ; four species of phytoplankton Bacillariophyta centrales : Melosira jurgensi; M. undulata ; Stephanopyxis turris ; Thalassiosira anguste - lineata ; T.decipiens and four species of epiphytic Baciliariophyta pennales : Achanthes flexella; Amphora bullatoides ; Asterionella formosa ; Navicula notha.Five diversity indices : relative abundance, Species richness; Shanon - Weiner diversity ; Jaccard similarity index and Sorinson similarity index were calculated in this study. Relative abundance index for phytoplankton was 44.5 at site 3 in April, 2012 while, epiphytic algae recorded the highest value was 91.6 on the shoot of C.demersum at site 1 in February, 2013 ; on the roots and leaves of T.domengensis in sites 2 and 3 were 66.6 in March, 2012 and 36.36 in June, 2012 on the leaves the same of plant. The highest value of species richness of phytoplankton was 21.9 at site 3 in April , 2012while, for epiphytic algae was 18.3 on the leaves of M.verticillatum and 9.0 on shoot of P.pectinatus at site 4 in March, 2012; on the leaves of C.desmersmum was 12.3 at site 2 ; on the shoot of M.verticillatum plant was the highest value at site 1 on May and June, 2012, while, on leaves and shoot of P. australis plants was the highest value in May and July, 2012 at site 2 and on root of P. australis and shoot of P. pectinatus at site 4 on August, 2012and February, 2013 at site 3, respectively. Results cleared a similarity with high value of Jaccard and Sorenson indices ranged between 3.2 to 50.0 and 55.87 to 153.21 respectively, while, the value of Shanon - wiener diversity index was ranged between 0 - 18.3 as high value which were 4.6 of M. verticillatum plant leaves at site 1 for May and June, 2012 and at low value during the period of study at sites 2, 3 and 4 for most plant parts. A statistical analysis was done using the canonical correspondence analysis(CCA).

تقييم بعض الدلائل الحيوية وصورة الدهون وعلاقتها مع مرضى السكري من النوع الثاني == Assessment of Some Biomarkers And Lipid Profile In Relation With Diabetic Patients Type 2

Author name: عذراء باقر حسن الشيباوي
Supervisor name: ارشد نوري غني الدجيلي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: لازال البحث مستمرعن ايجاد متغيرات جديدة قد تساعد في تشخيص ومتابعة مرض السكر وهو من المجالات المهمة في ميادين البحث العلمي حيث ركزت بعض الدراسات على حالة الانسولين بينمراكزت بحوث اخرى على صورة الدهون والشدة التاكسدية كاسباب للسكري. اجريت هذه الدراسة لمرض | The search of a new parameters for monitoring and even prediction of diabetes mellitus (DM) are still an important issue in many research fields. Some studies focused on the role of insulin status, while others concentrated on lipid disturbances or even oxidative stress disorders in the diabetes. This study was conducted on randomly selected 68 type 2 diabetic patients (27 Males and 41 Females) attending the diabetes mellitus center in Al - Sadder Teaching Hospital in Al - Najaf province, Iraq and a group of 20 apparently healthy subjects (10 Males and 10 Females) were included as a control group. The study was carried out from February 2013 to July 2013. The age of patients and control groups were range of 35 - 65years. The concentration of fasting blood glucose, cholesterol, triglyceride, LDL, VLDL, HDL, Apelin, Omentin, Vaspin, Visfatin and BMI were estimated in patients and control groups. The results show significant increase (P?0.05) in fasting blood glucose, cholesterol, triglyceride, LDL, VLDL, Apelin, Vaspin and Visfatin levels in patients compared with control groups, also the results show significant decrease (P?0.05) in HDL and Omentin level in patients compared with control groups. The results revealed that all biomarkers (Apelin, Omentin, Vaspin and Visfatin) not significant difference (P>0.05) in patients at different ages. The results also revealed that biomarkers (Apelin, Vaspin and Visfatin) levels increase significantly (P?0.05) in males than females in both patients and control groups, while the result of omentin level reveal no significant difference (P>0.05) between males and females in both patients and control groups. The results also revealed that significant increase (P?0.05) in BMI in patients compared with control groups. The results also show that biomarkers (Apelin, Vaspin and Visfatin) concentration increase significantly (P?0.05) with increasing BMI in males than females compared with control groups, while the result of omentin concentration show no significant difference (P>0.05) in patients and control groups and show lower significantly increase (P?0.05) in females than males compared with control groups. The results have been shown significant positive correlation (P?0.05) between biomarkers (Apelin, Vaspin and Visfatin) and FBG, cholesterol, triglyceride, LDL and VLDL in patients (males and females), while the results have been shown significant negative correlation (P?0.05) between biomarkers (Apelin, Vaspin and Visfatin) and HDL in patients (males and females). The results also have been shown significant negative correlation (P?0.05) between omentin and FBG, cholesterol, triglycerides, LDL and VLDL in patients (males and females), while the results have been shown significant positive correlation (P?0.05) between omentin and HDL in patients (males and females). The results also have been shown no significant correlation (P>0.05) between Vaspin and FBG in patients (males and females). The present study concluded that Apelin, Omentin, Vaspin and Visfatin levels maybe that could be adopted as markers for detection and diagnosis of diabetic patients type 2.

تقييم قابلية بعض انزيمات السليليز الفطري على تخمير تخمير بقايا النباتات لانتاج الايثانول == Evaluation of Capability of Some Fungal Cellulase In Fermentation of Plant Residues For Ethanol Production

Author name: عذراء حرجان محسن الدحيدحاوي
Supervisor name: فاطمة عبد الحسين التميمي | محسن هاشم رسن
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية الى تقييم قابلية بعض انزيمات السليليز الفطري على تخمير بقايا النباتات على انتاج الايثانول من بعض الفطريات المحلية المعزولة من 50 نموذج من التربة والذي تضمن Aspergillus oryzae , A. niger, A. terreus , A.flavus , A.fumigatus, A.parasiti | The present study was conducted to proceed a Bioethanol production using some of the waste fermentation plant by cellulosic hydrolysis enzyme produced by some local fungi isolated from 50 samples from soil which were including Aspergillus oryzae, A.niger, A.terreus , A.flavus , A.fumigatus, A.parasiticus, A.nidulans, Penicilliuum chrysogenum, Trichoderma longi, Rhizopus stolonifer, Cladosporium spp, Mucor indicus, Trichothecium spp. The prominent isolates were A. oryzae, A.niger, A. terreus which were chosen for further studies and screening to producing cellulase enzymes the fungal isolates revealed variation values of clear zone It’s (5.1, 5, 5, 4.8) cm to A. oryzae, A.niger, A. terreus , A.flavus respectively. The results revealed that the suitable carbon source to the cellulase activity was induced in different raw plant substrate, the highest was produced when using Corn cobs, Rice husk and Reed reached to (1.72, 2, 1.26)IU/ ml respectively when using A. oryzae and (1.24, 1.17, 1.89) IU/ ml when using A.niger, and (1.31, 1.19, 1.93)IU/ ml when using A. terreus. but using the cellulose powder and CMC, Avical sole carbon source total cellulase given least activity compare with raw plant substrate.on the other hand, using Mandels - Weber medium activity of enzyme production by A. oryzae, A.niger, A. terreus recorded (6.51, 4.14, 5.61) U/ ml respectively when using Corn cobs and (5.79, 4.03, 5.24) U/ ml and (5.55, 3.97, 5.19) U/ ml when using Rice husk and Reed by different fungi. The results refers that , the best chemical pretreatment results were appear when using NaOH at 100Co in 30min on Corn cobs, the total cellulase activity were (1.63, 1, 1.21) U / ml when using fungal isolated to degradation after treatment, while using hot water at100Co without NaOH in 15 min to pretreatment Rice husk activity were (2.16, 2.07, 2.12) U / ml but Reed are appear high activity when pretreatment with NaOH at 100C0 in 15min activity It’s (2.28, 1.65, 2.09) U / ml. The results revealed that, The optimized conditions of the enzymes were different in this study that give activities of Fpase, CMCase and ? - glucosidases to fungus A. oryzae compared to the rest of the fungi using Corn cobs as carbon source, it reached to(2.21, 2.30, 46.72) IU / ml respectively when pH6, and temperature tested for these enzymes recorded (1.64, 1.61, 30.86) IU\ ml respectively at a temperature of 30 C° and when using the concentration of substrate gave activity (2.4o, 2.48, 42.34) IU / ml of 6 % from Corn cobs and recorded when using nitrogen source concentration at 6 % (1.92, 1.89) IU\ ml in KNO3 and (NH4)2SO4 to Fpase and CMCase and the effectiveness of ? - glucosidase at the same nitrogen source (43.54, 50) IU / ml. on the other hand, showed enzymes Fpase, CMCase and ? - glucosidase high activities to ? - glucosidase, CMCase to fungus A. oryzae compared to the rest of the fungi using Reed as carbon source reached (2.31, 2.21, 46.15) IU/ml respectively at pH 6, and at different temperatures were (1.66, 30.94) IU/ml to Fpase and ? - glucosidase at 30 C°, but CMCase gave the highest efficacy (1.60) IU \ml at 25 C° and decreased effectiveness to (2.60, 2.57, 49.69) IU / ml at 6 % of the carbon source recorded effectiveness of the enzyme Fpase (1.19, 1.09) IU / ml when using (NH4)2PO4 and (NH4)2SO4 at 6 % as a nitrogen source of from either enzymatically CMCase and ? - glucosidase was effective at same nitrogen source (1.29, 2) and (35.19, 40.41) IU / ml respectively.The results showed when you use a Rice husk recorded higher effective enzymatic Fpase and CMCase and ? - glucosidases of fungus A. oryzae was (2.32, 39.27) IU /ml for Fpase and ? - glucosidases at pH 6 but CMCase gave the highest efficacy at 5 pH as recorded (2.04) IU / ml at temperatures 30 C°, reached to (1.64, 1.58, 29.13) IU / ml respectively for Fpase and CMCase and ? - glucosidases at a temperature of 30 C°, and when using 6% of the substrate was effective (2.47, 2.19, 44.86) IU / ml, activity recorded when using (NH4)2PO4 and (NH4)2SO4 at 6 % as a nitrogen source of (1.1) IU / ml to FPase on either enzymatically CMCase and ? - glucosidase was effective when use same a nitrogen source (1.18, 1.83) and (31.92, 36.91) IU/ml, respectively. In addition, The results refers when precipitation by ammonium sulfate specific activity were (38.69, 1.25, 5767.5) IU of the enzyme with 0.012 mg protein and then, Elution protein of crude enzyme solution of A.oryzae from DEAE - cellulose column was shown to the protein was separated in (33) fractions.It was found that only the fractions (F - 8) contained cellulase activity, while fraction (F - 30) eluted in column containing buffer and different concentrations of NaCl then using gel filtration and gave three peak of enzymes alone and Characterize enzymes purified FPase activity was found at 5.5 pH which (0.91) U and temperature was having activity at 25 and 30 C°, were (0.14, 0.15) U and CMCase found that the enzyme exhibited maximum activity at 25 C° was (0.33) U but 30 C° for ? - glucosidase was (38.22) U and the molecular weight of the protein was found to be about (38, 52 and 49) kD for (FPase, CMCase, ? - glucosidase) respectively. Finally, estimated ethanol density (0.80 - 0.91) g /ml and ethanol concentration (60 - 97) % but using chromic acid test of purified ethanol the complex enzyme with Reed, complex enzyme with Corn cobs recoded high value of ethanol which were (0.98, 1.72, 1.87, 1.89 ) % and (1.09, 1.83, 1.92, 1.99) % respectively but complex enzyme with Rice husk were given (1.01, 1.12, 1.48, 1.79) % in 4, 5, 6, and 7 days respectively on the other hand, when using the A.oryzae and A.terreus with Reed gave high ethanol value from other fungi with substrate in this study, and observed Rice husk in all results was recorded less absorbance at 350 nm and determination of ethanol concentration by gas chromatography mass used ethanol concentration 99 % as standard, also we showed the ethanol when using enzyme pure with substrate in which were (93.7, 97.1, 88) % ethanol to enzyme with Corn cobs and Reed and Rice husk respectively

دراسة مناعية ووراثية تتبعية لمرضى العقم من الرجال == A Prospective Immunological And Genetic Study of Infertile Men

Author name: علي عبد الزهرة مهدي الفحام
Supervisor name: يحيى كاظم السلطاني | عبد الزهرة كاظم محمد علي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: ان من الحقائق العلمية الثابتة اليوم ان الفحص الروتيني للسائل المنوي يعد عاملا تنبؤيا اجماليا للقدرة الاخصابية للرجال، ولذلك فان حاجة متزايدة بدات تبرز لاكتشاف فحوصات وظيفية جديدة في عملية تقييم العقم عند الرجال. ان الهدف الاساس من هذه الدراسة هو تعزيز الج | It is a scientific fact today that routine seminal fluid analysis is a key predictor of male reproductive potentiality ; so that there is an increasing need for finding out other functional tests in the assessment of male infertility. The main goal of the current study is to consolidate the scientific and practical efforts concerned with male infertility assessment especially from immunological and genetic aspects. The study was carried out between January 2013 and December 2013 including one hundred (100) selected infertile men who attended the Fertility Center in al - Sadr Medical City in Al - Najaf Al - Ashraf Governorate, the study also included twenty (20) healthy volunteer fertile men as a control. Serum and seminal antisperm antibodies (ASA) were determined by ELISA (Enzyme Linked ImmunoSorbent Assay) technique ; sperm chromatin condensation was evaluated by aniline blue staining (AB) ; sperm DNA fragmentation (damage) was evaluated by toluidine blue staining (TB). The results showed that the incidence and concentration of serum and seminal plasma ASA in the infertile men were significantly (p<0.05) higher than that in control fertile men. The incidence and concentration of serum and seminal ASA were also significantly (p<0.05) higher in normozoospermic infertile patients than that in control fertile men. There was a high significant negative correlation (p<0.01) between the concentration of serum and seminal plasma ASA in the infertile men and sperm motility and progressive motility, the concentration of serum and seminal ASA also showed a high significant positive correlation (p<0.01) with sperm agglutination, and a significant positive correlation (p<0.05) with seminal WBC count. The results also revealed that there were no significant effects (p>0.05) for patients' age, infertility duration, ABO blood groups and smoking habits on the levels of ASA in the serum and seminal fluid, while higher significant increase (p<0.01) in serum and seminal ASA concentrations was observed in infertile patients with varicocele as compared with those without. The results have also revealed that the percentage of sperm with chromatin decondensation and DNA damage has shown a high significant (p<0.01) increase in infertile patients compared to fertile men. There was also a significant increase (p<0.05) in sperm DNA damage in normozoospermic infertile patients comparing with fertile men, but no significant difference (p>0.05) was found in sperm chromatin condensation between normozoospermic patients and fertile men. Both sperm chromatin decondensation and sperm DNA damage showed a significant positive correlation (p<0.05) with sperm morphology, and a significant negative correlation (p<0.05) with sperm concentration, motility and progressive motility. The present study also revealed that the level of chromatin decondensation has been affected by patients' age, infertility duration, smoking habits and varicocele. It was observed that the higher percentage of chromatin decondensation was recorded in patients older than 37 years, and in patients subgroups with infertility duration more than 15 years, it was also higher in smoker as compared with non - smoker patients and in those with varicocele as compared with those without. Similarly, the level of sperm DNA damage has also been affected by patients' age, smoking habits and varicocele. It was observed that the highest percentage of sperm DNA damage was recorded in patients older than 37 years. The highest level of sperm DNA damage was also seen in smoker patients as compared with non - smokers and in those with varicocele as compared with those without. In contrast, the effect of infertility duration on sperm DNA damage was not statistically different (p>0.05). The correlation and regression results recorded high significant correlations (p<0.01) between serum ASA and seminal plasma ASA, and between sperm chromatin decondensation and sperm DNA damage. However, there was no significant correlation (p>0.05) between the level of serum and seminal ASA and each of sperm chromatin decondensation and sperm DNA damage. The current study concluded that the defects in sperm chromatin status (chromatin decondensation and DNA damage), and the immunological disorders caused by serum and seminal plasma ASA may - at least partially - contribute to the etiology of infertility of the patients under study, even in those with normal seminal parameters. However, it seems that ASA affect fertility in a pathway that is different from that affected by sperm chromatin defect. It was recommended that both sperm chromatin staining techniques and ASA tests could be routinely used as complementary tests to diagnose infertility.

تقييم وعزل فيروس الانفلونزا للانسان ودراسة الاستجابة المناعية في الجرذان التجريبية == Evaluation And Isolation of Human Influenza Virus And Studying Their Immune Response In Experimental Rats

Author name: فاديه مهدي مسلم العبيدي
Supervisor name: عبد المجيد عبد الله السعدي | يونس عبد الرضا الخفاجي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: This study has been conducted for the first time in Najaf / Iraq, and the study included the preparation of a local inactivated vaccine (whole and subunit).The number of cases infected with seasonal influenza virus were 647 cases out of one thousand suspected case. They were distributed into eleven groups. Seasonal influenza virus was detected by two diagnostic methods (rapid test device and real time PCR).The present study has reflected that the most diagnosed cases infected with seasonal influenza virus during the period extended from March 26, 2012 up to April 30, 2013 represented by were type A (H3N2) (283) cases. Whereas H1N1 (148) cases, H3N2+H1N1, (56) cases, H1N1 + H3N2 + B were (30) cases and 130 cases were infected with type B.Current study also revealed that age group of 46 - 60 years old infected with seasonal influenza virus were (159) case including (368) male and females were (279) of different infected group..The spread of seasonal influenza virus in Najaf varies from one region to another depending on the population density. Clinical cases were collected randomly during the months of the year. It has been found that the spread of disease has something to do with temperature and humidity as high humidity and low temperature lead to increased spread of the disease because of their relationship at the stage of the spread of epidemic influenza that spread from Dec 26, 2012 and up to April 1, 2013 in addition to expatriates infected..Two techniques were used for isolation of seasonal influenza virus first inoculation of embryonated chicken egg and secondly chicken fibroblast cell culture.It was found that at the embryonated egg lethal dosage (ELD 50 / 0.5 ml) (109.6) whereas tissue culture infective dosage (TCID50 / 0.1 ml) was (109.3) of (H1N1+ H3N2) and type (B) virus..Virus was purified by two ways that are depending on the vaccine type. The gamma irradiation that dosage used for inactivating vaccine of the purified virus which was extracted from allantoic fluid and tissue culture was (5.702) mSv/h for subunit virus vaccine type whereas gamma irradiation dosage for complete (whole) virus vaccine was (8.816) mSv/h. The results proved that immunoglobulins (IgM, IgA and IgG) levels as well as (IL - 17, IL - 10 and INF - 1?) indicated significant differences among vaccinated, infected and control groups.Subunit and whole virus vaccine extracted from chicken fibroblast cell culture are considered the best of derived from virus propagated in embryonated chicken eggs.Subunit virus vaccine is better than the whole virus vaccine derived from virus propagated in embryonated chicken eggs and extracted from chicken fibroblast cell culture.The current study also reflected that locally prepared virus subunit vaccine and purified whole virus killed vaccine capable of inducing humoral and cellular immune response in experimentally vaccinated rats..Histopathological changes appeared clearly on tissue sections (lung, trachea, heart, liver, spleen, kidney) of the infected group as compared with control and the vaccinated groups.

تاثير المعالجة المغناطيسية لمياه الري والسماد العضوي في نمو وانتاج القرنابيط botrytis var. Brassica oleracea صنف Solid snow == Effect of Magnetically Treatment of Irrigation Water And Organic Compost On Growth And Cauliflower Production Botrytis Cv. Solid Snow Var. Brassica Oleracea

Author name: كوثر عبد الجبار عبد
Supervisor name: ثامر خضير مرزة
General topic: Biology
Specific topic: Plant - Physiology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: ا جريت هذه التجربة في قسم علوم الحياة / كلية العلوم / جامعة الكوفة في محافظة النجف الاشرف وتضمنت مرحلتين : الاولى زراعة البذور لغرض قياس نسبة الانبات للمدة من 1 / 8 لغاية 8 / 8 / 2011 وانتاج الشتلات للمدة من 15/8/2011 ولغاية 14 / 9 /2011 في حقل خاص لانتا | This experiment was conducted in the Department of Biology, College of Sciences, University of Kufa in Al - Najaf Al - Ashraf province. It included two periods : the first was seed sowing to measure germination percentage from 1 - 8 till 8 - 8 - 2011, and transplants production from 15 - 8 - 2011 till 14 - 9 - 2011 in a private farm for vegetable transplants production. The second was transplanting in an open field belongs to Plant Production Department/Kufa Technical Institute from 15 - 9 - 2011 till 17 - 1 - 2012 to measure plant growth parameters and curd cauliflower production cv.solid snow in harmless environment techniques. The experiment was adopted as factional experiment. The treatments were distributed according to Randomized Complete Block Design (RCBD) with four replicates. The experiment included the study of two factors : the first, was five magnetic intensities for treating waters magnetically (0; 500; 750; 1500 and 2500 Gauss) by means of dipolar magnetrons. The second was three quantities of plant organic manure(rice residues).i.e.(0, 2.5 and 5.0 kg/furrow) with a length of 6.5 m. and their interactions on growth and production the yield of cauliflower, cv.solid snow. Duncan's Multiple Range Test (DMRT) was used to compare means at probability of0.05. Correlation and regression coefficient were also used to evaluate the relationships between studied parameters and time. Vegetative growth parameters that are measured were : plant leaf number ; stem height; stem diameter; plant leaf area; leaf fresh and dry weight; stem dry weight; plant shoot fresh and dry weight. Flowering parameters were : curd fresh and dry weight and curd diameter. The results could be summarized as following : 1. Water chemical analysis showed that irrigation with magnetized treated water had an effect on physical properties, nevertheless, was more or less affected ions components. 2. The use of magnetized treated water in irrigation resulted in an increase in germination percentage of cauliflower seeds cv. Solid snow.The highest germination percentage was obtained from magnetic intensity of 1500 Gauss in both experiments, laboratory and field that reached 71.14 and 65, 62%, respectively on the base of Arc Sin. 3. The use of magnetized treated water in plant irrigation at all the used intensities resulted in an early emerged curd cauliflower by 15 days compared with control treatment plants. 4. 750 gauss magnetized treated water achieved at age of 138 days from transplanting, the highest plant leaf area mean is (4836.2 cm2) with percentage increase of 51.7% compared to control treatment plant leaf area (3188.3 cm2).That effect was reflected on curd weight. 5. 1500 and 2500 Gauss magnetized treated water treatments at earlier ages increased the values of vegetative growth parameters which reflected its effect on flowering growth parameters by shorter time of curd irritation, compared with the effect of 500 - 750 Gauss at earlier ages , which are in spite of its late effects produced at 150 days old from transplanting particularly 750 Gauss the highest mean of curd fresh weight. 6. 750 Gauss magnetized treated water achieved the highest yield reached 22.2 ton/h. which differed from that productivity gained from control treatment plant(9 - 29 ton/h.) with percentage increase of 138.8%. 7. Organic plant manure addition at both quantities(2.5 and 5.0kg/furrow) resulted in an increase in both vegetative and flowering growth parameters compared with untreated plants. 5 kg/furrow treatment produced the highest means compared to control treatment particularly at the age of 138 days in all vegetative growth parameters. 8. The addition of 5 kg/furrow at age of 150 days produced the largest curd fresh weight 918.4 g. and diameter of 17.2 cm which statistically differed to compared plant treatment that produced 506.0 g. and 13.3 cm. for curd fresh weight and diameter at the same age respectively. 9. 5 kg/furrow organic manure addition resulted in a highest productivity of 21.6 ton/h. compared to that of control treatment of 11.9 ton/h. with an increase of 81.5%. 10. Interaction between irrigation water magnetically treated with 750 Gauss and addition of (organic manure with 5 kg/furrow) at the age of150 days resulted in the highest curd fresh weight of 1292.9 g. with curd diameter of 20.3 cm. compared to that plant of control treatment produced curd fresh weight of 292.5 g. and 11.0 cm. diameter. This interaction also produced 30.3 ton/h. for (750 Gauss×5 kg/furrow) compared with the productivity of control treatment plants that gave 6.86 ton/h. with percentage increase of 341.6%

دور الفيروس المضخم للخلايا CMV في مرضى التهاب الكبد الفيروسي ذاتي المناعة في حالات التهاب الكبد المزمن نمط B == The Role of CMV In Autoimmune Hepatitis Among Chronic Cases of Hbv

Author name: هـدى جميل باقر الخلخالي
Supervisor name: محمد عبود محسن | محمد عبد كاظم السعدي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: هدفت الدراسة الحالية الى تشخيص التهاب الكبد الفايروسي نمط (ب) وتشخيص التهاب الكبد ذاتي المناعة في المرضى المصابين بالتهاب الكبد.ودور الاصابة بالفايروس المضخم للخلايا البشرية CMV في حث التهاب الكبد ذاتي المناعة.من مجموع 360 حالة مشبه بها جمعت من مستشفى ال | This study aimed to investigate the existence of hepatitis B virus and autoimmune hepatitis in hepatitis B patients as well as to detect the role of cytomegalovirus in the induction of AIH disease. A total of 360 suspected cases were collected from Center Health laboratory/Al - Hakeem Hospital, and AL - Sadder medical city in AL - Najaf city, during the period from January (2013) to August (2013). Only 76 were seropositive hepatitis B (55 males and 21 females with age ranging 11 - 72 years).In addition, 15 healthy individuals without any evidence of chronic inflammatory disease were depended on the control group, age ranging 21 - 50 years.Blood samples were collected from patients and healthy controls were tested for HBsAg and Anti - HBc Ab using Enzyme Linked Immunosorbent Assay (ELISA) technique to investigate hepatitis B seropositive and chronic hepatitis B respectively. For investigated AIH disease was performed depending on the Line Immune Assay technique. While for detection CMV were initially identified by serological technique (ELISA, MiniVIDAS) to detect anti - CMV IgM and anti - CMV IgG; then confirmed employment molecular technique using Real Time Polymerase Chain Reaction for the detection the presence of DNA of CMV. Samples were collected from patients and control to estimation immunological level (C3, C4, IL - 10 & TFN - ?) by using ELISA and radial immunodiffusion method.The results showed that 76 HBsAg seropositive in all age groups but the age group 44 - 54 year revealed high significance (p<0.05) than other age groups. While 35 out of 76 seropositive with HBc Ab, the age group 55 - 65 years showed high significance (p<0.05) than other age groups, and males more infection than females. The result also revealed that the AIH disease was 5 out of 76 patients infected with Type 1 AIH. Included 2(40%) have demonstrated the infection HBsAg positive with reactivation CMV While 3(60%) infected with HBsAg only.The results showed that 68 out of the 76 samples were positive for anti - CMV IgG antibodies, and 4(5.26%) samples were positive for anti - CMV IgM. While the MiniVIDAS test results showed 73 out of the 76 samples was positive for anti - CMV IgG antibodies. 2(2.6%) were positive for anti - CMV IgM antibodies. The results of the Real - Time PCR revealed that DNA of CMV were detected in 23 out of 76 patients were found in all age groups with viral loads ranging from (0.24 - 1730000) Copies/ml, and the results of controls group in Real - Time PCR were CMV negative. The results of cytokines (TNF - ?) showed a high significance (P<0.05) elevation in the serum of all patients than control (419.3 ± 27.8) pg/ml, and the results that AIH showed increase in cytokine level was (1218.2±44) pg/ml than other patients. Whereas chronic hepatitis B patients recorded high significance (P<0.05) in level (IL - 10) was (901.5±22.2) pg/ml than other patients and control (373 ±30.3) pg/ml. According to sex no - significance difference between males and females in results of cytokines profile (IL - 10, TNF?). Complement fraction C3 decreased in all patients compared to those healthy control, while the AIH patient recorded high significance (P<0.05) 142.2±8 mg/dI than the other patients. In regard to C4 was revealed normal level in all patients compared with control groups while in the AIH revealed high significance (P<0.05) was 41.7±5.1 mg/dI compared with other patients and no - significance difference (P<0.0) between males and females in level of C3and C4.The overall finding results showed that the activation cytomegalovirus with hepatitis B virus Contribute to the induction of AIH and cause immune suppressor for them by increase and decrease many immune factors.

تقييم مستوى الثرومبوسبوندين لدى مرضى السكري النوع الثاني == Assessment of Thrombospondin Level In Diabetic Patients Type II

Author name: وجدان راجح حمزة الكريطي
Supervisor name: ارشد نوري غني الدجيلي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: The present study is intended to asses serum levels of Thrombospondin - 1, Fasting blood glucose, Glycated Hemoglobin A1c, Lipid profile and BMI in type 2 diabetic patients, also correlation between Thrombospondin - 1 with all criteria above in both males and females.The study was conducted on randomly selected 65 type 2 diabetic patients (34 males and 31 females) attending the diabetes mellitus center in Al - Sadder Teaching City in Al - Najaf province, Iraq and a group of 24 apparently healthy subjects (12 Males and 12 Females) were included as a control group. The Study was carried out from August 2013 to February 2014.The patients' age was ranging of 35 to 64 years old.The results indicated a significant increase (p<0.05) in serum FBG, Cholesterol, TG, VLDL - C, LDL - C, TSP - 1 levels and a significant decrease (p>0.05) in serum HDL - C level in diabetic patients in comparing with healthy groups. The results also revealed that significant increase (p<0.05) in serum HbA1c level and BMI in diabetic patients in comparing with healthy groups.The results also revealed no significant differences (p> 0.05) in serum Cholesterol, Triglyceride, VLDL - C, LDL - C, HDL - C and TSP - 1 levels between males and females in both patients and health groups, while the results of FBG and HbA1c levels increase significantly (p<0.05) in females than males in both patients and health groups.The results show that Cholesterol, Triglyceride, VLDL - C, LDL - C, TSP - 1, FBG and HbA1c levels increase significantly (p<0.05) in both males and females patients groups in comparing with males and females of healthy groups respectively, and a significant decrease (p<0.05) in serum HDL - C in both males and females patients groups in comparing with males and females of healthy groups respectively.The results indicated a significant increase (p<0.05) in serum TSP - 1 level in females than males patients at same BMI (over weight and obese) except normal weight there is no significant differences (p>0.05) and there is no significant differences (p>0.05) between males and female of control groups at same BMI.The results show that TSP - 1 increase significantly (p<0.05) with increasing age of patients in males and females patients and the ages (55 - 64y) are highly significant(p<0.05) than (45 - 54y) and (35 - 44y), But there is no significant differences (p>0.05) in males and females of healthy groups at different ages. while there is a significant increase (p<0.05) in serum TSP - 1 level at same ages in both males and females patients in comparing with males and females of healthy groups respectively. The results also indicated that TSP - 1 increasing significantly (p<0.05) with increasing duration of disease in males and females patients and the (11 - 15y) reveals a highly significantly (P<0.05) than (6 - 10y) and (1 - 5y). The results have been shown significant positive correlation (P<0.05) between TSP - 1 and FBG, TSP - 1 and HbA1c, TSP - 1 and BMI, TSP - 1 and cholesterol, TSP - 1 and TG, TSP - 1 and LDL - C, TSP - 1 and VLDL - C in (males and females), males, females DM patients. The results have been shown significant negative correlation (P<0.05) between TSP - 1 and HDL - C in (males and females), males, females DM patients. The present study concluded that Thrombospondin - 1 and HbA1c levels were markers for detection and diagnosis of diabetic patients type

تحديد الصفات المظهرية والوراثية لانواع بكتريا المكورات المعوية المعزولة من المرضى في محافظة النجف الاشرف == Phenotypic And Genotypic Detection of Enterococcus Sp. Isolated From Patients In Al - Najaf Al - Ashraf Governorate

Author name: زهراء حميد عودة القريشي
Supervisor name: مهدي حسين محيل العمار
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: The study aimed to isolate and identify the Enterococcus spp. from different clinical specimens and study the virulence factors of predominant species, as well as detected the virulence factors encoding genes such as efaA (endocarditis - associated antigen), esp (enterococcal surface protein), eep (stimulating of pheromones expression) and enlA (enterolysin A) genes by PCR techniques.There were three hundred clinical specimens collected from patients suffering from different clinical infections during the period from September 2013 to January 2014 in AL - Sadder Medical City and AL - Hakem General Hospital. The identification of the Enterococcus spp. isolates were depended on colonial morphology, microscopic examination and biochemical tests as a primary identification. The final identification was performed with the automated VITEK - 2 compact system using Gram positive - Identification (GP - ID) cards.According to the results obtained by the VITEK tests forty two clinical isolates of Enterococcus were detected, which distributed into : (22) isolates from urine, (8) vaginal swabs, (6) seminal fluid, (4) throat swabs and two isolates from wound swabs with no isolates from cerebral spinal fluid, stool and blood specimens.This study revealed that the E. faecalis is more distributed with 25(59.52%), followed by E. faecium with 10 (23.80%), E.avium with 5 (11.90 %), E. durans and E. raffinosus with 1(2.39%) for each.The study investigated the virulence factors of E. faecalis, E.faecium and E.avium, which play a major role in enterococcus pathogenicity. E. faecalis and E.faecium had the ability to producecapsule, gelatinase, biofilm, adhesion, protease, bacteriocin, haemolysin and cytolysin except ? - lactamase produced only by E. faecalis while E.avium produced all these virulence factors except gelatinase, bacteriocin and cytolysin.The results revealed variation in the resistance of bacteria to antibiotics, E.faecalis express absolute resistance (100%) to Erythromycin, high resistance against Gentamycin, Tetracyclin and Vancomycin but high susceptibility to Ciproflaxacin and Penicillin and moderate susceptibility to Chloramphenicol. E.faecium exhibited absolute resistance (100%) to Erythromycin, Gentamycin and Tetracyclin, high resistance against Chloramphenicol, Penicillin and Vancomycin but high susceptibility to Ciproflaxacin while E.avium exhibited susceptibility (100%) to all these antibiotics except Tetracyclin showed absolute resistance.Then detected the virulence factors encoding genes : efaA, esp, eep and enlA genes by using PCR techniques and Electrophoresis Systems.Finally, The genotypic method. Regard to genotypic study the outcome showed that 21(84%) isolates of E.faecalis, 4(40%) of E.faecium and 5(100%) of E.avium were carrying efaA gene and 17(68%) of E.faecalis, 5(50%) of E.faecium, 5(100%) of E.avium were carrying esp gene while eep gene was carrying only by E.faecalis and E.faecium ; 12(48%) and 3(30%) respectively. Also the results revealed that only 1(4%) isolates of E.faecalis have enlA gene.

التحري عن بعض المؤشرات المناعية وارتفاع نسبة وجود الفيروس HCMV في المرضى المصابين بالفشل الكلوي == Assessment of Some Immunological Markers And Viral Load For Hcmv In Patients With Renal Failure

Author name: احمد جاسم شوالة الخويلدي
Supervisor name: مهدي حسين محيل العمار | زياد متعب الخزاعي
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: اجريت هذه الدراسة على 150 مريضا الراقدين في مستشفى الصدرالتعليمي ومستشفى الحكيم (قسم الكلى) في محافظة النجف خلال الفترة الممتدة من كانون الاول 2012 الى شهر اب 2013. وكان الغرض من هذه الدراسة بيان علاقة الفيروس المضخم للخلايا بمرض الفشل الكلوي، تراوحت اعما | This study was carried out on 150 renal failure patients, who were admitted to the kidney department in AL - Sadder Medical City and Al - Hakeem hospital in AL - Najaf governorate during the period from December 2012 to August, 2013. Subjects of this study were Investigated for the role of Cytomegalovirus among them, their age ranged between (1 - 88) years. Twenty four (age - and sex - matched) healthy individuals without any evidence of chronic inflammatory disease depended as control. All patients and control divided in four age groups. Blood and urine samples were collected from patients and control for immunological (IgM, IgG, C3, C4, IL - 6, IL - 10, IL - 12 & IFN - ?) by using ELISA and molecular study by RT - PCR, respectively. The obtained results showed that HCMV - IgG antibody was 100% for all cases, while IgM was 18.66% compared with that of control. Real time - PCR amplification for presence of HCMV DNA in urine samples revealed that HCMV genome were detected in 22(14.66%) of the 150 urine samples in all age groups, that distributed into 12(21.05%) with viral load ranged (20 - 543840) Copy/ml in females and 10 (10.75%) with viral load ranged (40 - 28050) Copy/ml for males. The results of cytokines profile showed a highly significant(P<0.05) elevation in patients than control. According to sex females appeared higher IFN - ?, IL - 6, IL - 10 ( 83.86, 82.67, 9.06 pg/ml, respetively) than males were (76.57 , 79.36, 6.51 pg/ml, respectively). Where's IL - 12 were recorded higher elevation in male (28.83 pg/ml ) than females (27.35 pg/ml). According to age groups 41 - 60 age group showed high level in IFN - ?, IL - 6, IL - 10 were (96.45, 90.40, 9.62) pg/ml , respectively.While IL - 12 appeared high level (37.43 pg/ml ) in age group 1 - 20 years Complement fractions C3, C4 decreased in all groups of patients compared to those of a healthy control. C3 was recorded (64.37 mg/dl) in females, and (70.24 mg/dl) in males and decreased to (63.41 mg/dl) in age group (1 - 20) years, while C4 decreased significantly among sex it was (21.06 mg/dl) in females and(24.22 mg/dl) in males, and down to (21.65 mg/dl) in age group (21 - 40) years.The overall finding results showed that HCMV are more prevalence among chronic renal failure patients and cause immune suppressor for them by increase and decrease many immune factors.

دراسة جزيئية لعوامل ضراوة المكورات النعقودية السالبة لانزيم التجليط والمعزولة من اصابات مختلفة == Molecular Study of Virulence Factors of Some Coagulase Negative Staphylococci Isolated From Different Infections

Author name: سعاد عبد الهادي عبد الرزاق الحلو
Supervisor name: عباس شاكر جواد المحنة
General topic: Biology
Specific topic: Microbiology - Enzymes
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: The study aimed to investigating the role of coagulase - negative staphylococci in human infections, and determining the predominance genes of the virulence factors. Three hundred clinical specimens were collected from out and inpatients undergoing catheter related infections and twenty specimens were collected from healthy hospital staff as a control from January 2013 to July 2013 of Al - Zahraa Teaching Hospital, Al - Sader Teaching Hospital and Al - Hakeem Hospital in Al - Najaf Al - Ashraf province. The specimens were included urine, blood, vaginal swabs, seminal fluid and wound swabs. The specimens were cultured on mannitol salt agar and the primary identification was depended on Gram stained and biochemical tests. Then finally identification with Vitek 2 system is done.One hundred isolates were identified as coagulase - negative staphylococci (CoNS), Staphylococcus haemolyticus was identified as the most frequently isolated species in (53%), followed by Staphylococcus epidermidis (26%) and Staphylococcus hominis were recorded in (21%). Most of CoNS isolates were highly resistance to penicillin G (benzylpenicillin), oxacillin, cefoxitin and erythromycin; and low resistance to rifampicin, levofloxacin and others. While, control isolates results showed moderate resistance to penicillin G and erythromycin; low - level of resistance to cefoxitin, oxacillin and other antibiotics.The investigation of virulence factors revealed that 93% of coagulase - negative staphylococci isolates were production of slime layer, DNase 58%, protease 29% and hemolysin 88%. But the results gave negative result for TNase and lipase enzymes.Monoplex and multiplex PCR were used to explore the MecA, aap, icaA, icaD, atlE, sea, seb, sec, sed, hla, hlb, sspA, sspB, geh, nuc genes. The results showed that all CoNS isolates (100%) had mecA and atlEgenes, but 98% of isolates had aap, 93% icaA and icaD genes. PCR revealed that only (14%) of isolates had genes for enterotoxins expression. (92.86%) and (7.14%), sea and seb respectively, in contrast, the sec and sed genes were not be recorded.The result showed that 47% of CoNS isolates had hla gene and 41% contain hlb gene, 29% were positive for the sspA gene whereas the sspB gene and geh and nuc2 genes not found in any of staphylococcal isolates. Finally, the result indicated that 58% of CoNS isolates were expressed the nuc1 gene.Plasmid curing was carried out in order to determine the origin of resistance and some virulence factor genes (chromosomal or plasmid - borne gene). The curing (elimination) of the plasmids of coagulase - negative staphylococci isolated was catalyzed using ethidium bromide in different concentration and high temperature (44?C). The results showed that the oxacillin resistant coagulase - negative staphylococci were plasmid mediated since 93% of the isolates showed negative result on oxacillin resistance screening agar, and absence of mecA gene from all isolates. Also, 41 of coagulase - negative staphylococci isolates that showed ? - hemolysin became non - hemolysin after manipulated with ethidium bromide.Finelly, taking into consideration the etiological importance of CoNS has often been neglected, the present investigation confirmed that these microorganisms should not be ignored or classified as mere contaminant.

اكتفين ا كعلامة بيولوجية للحمل خارج الرحم والاجهاض المنسي == Activin A As A Biomarker For Ectopic Pregnancy And Missed Abortion

Author name: مي حامد حسن
Supervisor name: نورهان شاكر
General topic: Medicine
Specific topic: Obstetrics and Gynecology
Degree: Higher Diploma
Language: English
University location: Najaf
First pages:
Abstract: Background : Activin A is a member of transforming growth factor B family that is secreted from ovary and placenta, however, in pregnancy the main source is the trophoblast.Aim of study : Activin A measurement to differentiate between intrauterine pregnancy and failed (missed abortion and ectopic) pregnancies.Study design : a Case control study.Setting : Department of Obstetrics and Gynecology in the College of Medicine Kufa University in Al Zahraa Teaching Hospital.Material and Methods : This study is a case control study consisting of 90 patients on three groups : Group 1 ectopic pregnancies (EP) (n=30) , Group 2 missed abortions (MA) (n=30) and Group 3 healthy viable intrauterine pregnancies (IUP) (n=30) were came to hospital with lower abdominal pain and\or vaginal bleeding or both between 6 - 8 weeks of gestation. Serum samples were sent at initial visit before treatment for measurement of activin A using ELISA test.Results : Activin A concentrations were significantly lower in women with EP (n = 30, mean value of 89.9 pg/mL) and women with MA (n = 30, mean value of 164.9 pg/mL) compared to IUP (n = 30, mean value of 334.5pg/mL); P < 0.001. Activin A can discriminate an ectopic pregnancy from viable pregnancies also, it was able to discriminate a MA from an EP.Conclusion : In this study activin A could be considered a promising biomarker for the discrimination between an IUP and a failed pregnancy (MA or EP).

مستوى مصل الدم للهرمون مضاد مولر AMH كاداة تشخيصية للاستجابة لتحريض الاباضة في مرضى متلازمة المبيض المتعدد الكيسات PCOS == Serum Level Of AMH As A Prognostic Tool For Response To Ovulation Induction In Pcos Patients

Author name: فرح حسام الدين سعران
Supervisor name: صالحة علي حسين | بتول عبد الواحد
General topic: Medicine
Specific topic: Obstetrics and Gynecology
Degree: Higher Diploma
Language: English
University location: Najaf
First pages:
Abstract: متلازمة المبيض المتعدد الكيسات (PCOS) هواضطراب شائع جدا التي تحدث في 4? الى 7? من النساء في سن الانجاب. من المعروف انPCOSغالبا ما يترافق مع الاصابة بالامراض التناسلية وزيادة خطرالاصابة بسرطان بطانة الرحم،والتشخيص له اهمية خاصة لانه يعتقد الان بانPCOS له ا | From the observation that some PCOS patients were known to have high serum level AMH and it has been shown to be useful in its diagnosis, however; the clinical significance of high serum AMH level in prediction of ovarian response to medical therapy was under studied we are doing this study aiming to test the value of serum AMH level as a marker of sever disease and may be subsequent resistance to medical therapy. (AMH) has glycoprotein dimmer structure.AMH is produced by the granulose cells. AMH level in the plasma of PCOS patient are two or three times higher than average and begin to decline five years later than healthy womenObjectivesThe main objective of the study is to estimate the AMH level as a prognostic factor in PCOS infertile women on ovulation induction, also to describe some risk factors of infertility and their association with the AMH level.MethodologyA cross sectional study has been carried out in the outpatient clinic at Al Sadr infertility center under supervision of specialist gynecologist on 60 patients. The data has been collected by a questionnaire which contains demographic characteristics, gynecological history, physical examination and some laboratory investigations. The data collected by simple random to select the case from the outpatient clinics in the fact of 4 - 5 infertile women one day/week, then a period of time required for following up them after the ovulation induction The data have been collected during the period from the 1st of February till the 30 of September 2015.ResultsThere was a high statistically significant difference (P 0.000) in mean AMH among the cases with negative PT from those with positive PT, also the higher level of AMH strongly linked (P 0.023) with the presence of acne as a symptom of PCOS. There was no correlation of AMH with the age, LH, FSH, prolactin, TSH, size of dominant follicle and infertility duration. While it was positively correlated (P 0.000) with the age of menarche, BMI (P 0.000), Serum testosterone (P 0.002) andendometrial thickness (P 0.000).Conclusions & RecommendationsAMH is a useful test to study folliculogenesis and ovarian potential in various situations of infertility and for identification of PCOS. High levels of AMH are commonly associated with pregnancy failure.So, a recommendation of AMH level measuring for all PCOS patients should be done in order to know the prognosis during the ovulation induction.

العلاقة بين البروتين التفاعلي عالي الدقة في حالات سمدمية الحمل مع او بدون تخلف نمو الجنين == Associationbetween Highly Sensitive C - Reactive Protein Level In Cases Of Preeclampsia With Or Without Intrauterine - Growth Restriction

Author name: روسم جاسم محمد
Supervisor name: الاء محمد صادق
General topic: Medicine
Specific topic: Obstetrics and Gynecology
Degree: Higher Diploma
Language: English
University location: Najaf
Key words:
  • العلاقة بين البروتين التفاعلي عالي الدقة في حالات سمدمية الحمل مع او بدون تخلف نمو الجنين
First pages:
Abstract: سمدم?ةالحملھوخلل?ص?بتقر?با 4% منالنساءاثناءالحملوتترتبعل?ھمخاطركث?رةبالنسبةل?موالطفل. نعرفتسممالحملعللىانھا?صابةالحد?ثةغ?رالمسبوقةبارتفاعضغطالدماثناءالحملبعدا?سبوع 20 منالحملوالمصحوبةبوجودبروت?نفيالبول.البروت?نالتفاعليعاليالدقةھومؤشرك?م?ائيبامكانناق?اسھف | Preeclampsia is a hypertensive disorder that affects 4% of pregnant women , in which the exact cause cannot identified it is one of the major causes of maternal and fetal morbidity and mortality as it causes death of more than 6300 women annually , also it puts a burden on perinatal outcome as prematurity and Intrauterine growth restriction.Continuous search for predictive markers of severe PE is important and can be used to target high risk women for effective preventive treatment.Aim of study : to evaluate that Highly Sensitive C - Reactive Protein can be used as maker for severity of PE and whether it can be used to predict development of Intrauterine growth restrictionPatients and methods : this is convenient a cross - sectional study includes 80 pregnant women with their age 18 - 35 years , their gestational age 32 - 40 weeks , admitted to AL - Zahraa teaching hospital , whose diagnosed as PE based on BP and proteinurea. Full history and clinical examination were performed, venous blood aspirated for each women for biochemical analysis, Liver functions tests, Renal function tests Highly Sensitive C - Reactive Protein, and Doppler ultrasound.Results : The patients divided into those with mild PE and severe PE, with or without IUGR. No significant difference between the level of hsCRP and the severity of PE as p value was(0.779), but there is significant difference between the presence of IUGR and the level of hsCRP when it is 2mg/L as the p value was(0.020).Conclusion : We conclude that s.hsCRP is not sign

دراسة علاقة طول عنق الرحم المقاس بالسونار الداخلي مزامنة مع كمية السائل الامنيوسي في توقع وقت حدوث الولادة المبكرة خلال سبعة ايام من تمزق غشاء السائل الامنيوسي المبكر == Transvaginal Cervical Length & Amniotic Fluid Index : Can They Predict Delivery Latency Following Preterm Premature Rupture Of Membrane

Author name: وسن غائب حسون غائب
Supervisor name: امال منير مبارك
General topic: Medicine
Specific topic: Obstetrics and Gynecology
Degree: Higher Diploma
Language: English
University location: Najaf
Key words:
  • علاقه طول عنق الرحم المقاس بالسونار الداخلي مزامنة مع كمية السائل الامنيوسي في توقع حدوث الولادة المبكرة خلال سبعة ايام من تمزق غشاء السائل الامنيوسي المبكر
First pages:
Abstract: توقع حدوث الولادة عند الحوامل المصابات بتمزق غشاء السائل الامنيوسي المبكريساعد الحامل على تهيئه نفسها لدخول المستشفى لاجراء اللازم لها واعطاءالجرعةالمنشطةلرئة الطفل وادخاله الى وحده العناية المركزة لحديثي الولادة.هدف الدراسة : تهدف الدراسة الى قياس طول عن | Patients with PPROM are often hospitalized for a prolonged period of time and deliver premature infants who frequently require neonatal intensive care. These women and their infants contribute significantly to obstetric &neonatal health care costs. The prediction of delivery latency could help direct the need for specific interventions such as hospitalization, intensive monitoring, timing of antenatal steroids.Objective : To determine whether transvaginal CL, AFI,or a combination of both can predict delivery latency within 7 days in women presenting with PPROM.Methods and Study Design : This was a prospective observational study of TVCL measurements in 80 singleton pregnancies with PPROM between 24 - 34 weeks. Transvaginal ultrasonography was performed with TOSHIBA SSA - 340A(Tokyo - Japan) apparatus 7.5MHz transvaginal probe, to measure the CL&AFI. Delivery latency was defined as the period from the initial TVCL after PPROM to delivery of the baby with our primary outcome being delivery within 7 days of TVCL. The independent predictability of significant characteristics for delivery within 7 days was determined using multiple logistic regression.Sensitivity, specificity, and predictive values were used to determin, whether the presence of a short TVCL, AFI or a combination of both affected the risk of delivery within 7 days.Result : Our study in women with PPROM show the validity of CL in predicting labor when the cut off value2cm the sensitivity=52.6%, specificity=69%, PPV=60.6%, NPV =61.7% and Accuracy=61.25%.The validity of AFI when the cut off value 5cm the sensitivity=71.1%, specificity=50%, PPV=56.3%, NPV=65.6% and Accuracy=60%.With combination of CL&AFI in predicting time of labor after PPROM the sensitivity=50%, specificity=92.8%, PPV=86.4%, NPV=67.2% and Accuracy 72.5%.In women with PPROM the rate of gestational age ? 30 weeks that labored within 7 days 44.7% and those labored more than 7 days 55.3%.Also the rate of gestational age >30 weeks that delivered within and after 7 days are 50%.According to the parity we found the mean value of those delivered within 7 days are 1.24 and those after 7 days are 1.28.Conclusion : Our study shows there is increase in positive predictive value when combination of AFI and CL in prediction of time of labour so women with AFI?5 and CL?2 had 86.4% risk of delivery within 7 days after PPROM. Also the study found there was no significant association between gestational age, or parity with the prediction time of labor.

نسبة حدوث النزف بعد الولادة في حالات الولادات الطبيعية, الاصطناعية, القيصرية الطارئة والغير طارئة في مستشفى الزهراء التعليمي لسنه 2015 == The Incidence Of Postpartum Hemorrhage After Spontaneous And Induced Vaginal Delivery Versus Elective And Emergency Caesarian Section At Al - Zahraa Teaching Hospital In 2015

Author name: اسراء علي عباس
Supervisor name: ازهار موسى الطريحي
General topic: Medicine
Specific topic: Obstetrics and Gynecology
Degree: Higher Diploma
Language: English
University location: Najaf
Key words:
  • حدوث النزف بعد الولاده في حالات الولادات الطبيعيه
  • الاصطناعيه
  • القيصريهالطارئه والغير طارئه في مستشفى الزهراء التعليمي لسنه
First pages:
Abstract: خلال هذا البحث تم رصد حدوث النزف المهبلي بعد الولاده ومقارنته بين اربع مجاميع من المريضات اللاتي دخلن الى مستشفى الزهراء التعليمي من فتره الثاني من اذار الى الثاني من اب وكان عدد المريضات 9674 مريضه وكان عدد الولادات الطبيعيه 6137 منها 1352 كان عن طريق تح | The labour records of the patients admitted to the labour word and obstetrics word and theater in Al Zahra teaching hospital (9674 patients) during period of 5 months from 2nd of March to the 2nd of August, consequence study to compare the incidence of postpartum haemorrhage after : 1 - Spontaneous labour2 - Induced labour 3 - Elective caesarian section4 - Emergency caesarian sectionObjective : To compare the incidence of haemorrhage and risk factors among certain group of patients and to discover which group is more liable to develop PPH.Study design : Cross sectional studyMethodology : Estimating the blood loss for assessment of PPH by gravimetric method and collection of soaked packs method, evaluate the severity of bleeding and put the women in the proper PPH category to do the proper management plans.Finding and conclusion : The finding of our research is the incidence of PPH is 1.64% among all types of labour (159 patients had PPH during this period) and the main risk factor for PPH in study is the primigravidas in induced or augmented labour.Practical implication : These finding indicate that PPH is another complication of induction that needed to be taken into account when induction in being considered

تاثير معايير النطف ونضوج مادة الصبغين على نتائج عملية الحقن المجهري == Impact of Sperm Parameters And Sperm Chromatin Maturity On Intracytoplasmic Sperm Injection Outcome

Author name: هند عبد الكاظم
Supervisor name: يحيى كاظم السلطاني
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: يعتبر العقم من المشاكل المتعلقة بصحة الانسان ويؤثرعلى سدس الازواج في عمر الانجاب تقريبا. يعد الذكر مسؤلا عن خمسين بالمئه من مجموع الحالات.الوظيفه الرئيسيه لتقنيات الانجاب المعان هي علاج العقم بما فيها حالات العقم الذكري وزيادة فرص الحمل من خلال التغلب عل | Infertility is a health concern affecting nearly 17% of young couples in their reproductive age. Male factor infertility accounts for 50% of all cases. The key responsibility of assisted reproductive technologies (ART) is the management of male infertility by bypassing seminal fluid disorders. Defective Sperm parameters were responsible for deciding the fertility potential and related to pregnancy rate in vivo as well as in vitro.Today, the classic seminal fluid analysis is found to be of limited importance in describing a couple’s fertility status. There is now clinical evidence to show that damage to human sperm DNA may adversely affect reproductive outcomes and the spermatozoa of infertile men possess substantially more DNA damage than do spermatozoa of fertile men and there appears to be a threshold of sperm DNA damage beyond which embryo development and pregnancy are impaired.In view of that, the aims of the present study were : Evaluation of the influence of defective sperm parameters including concentration, morphology and motility on intracytoplasmic sperm injection (ICSI) outcome represented by fertilization rate, cleavage rate, embryo quality and pregnancy rate. Assessment of the sperm chromatin maturity in infertile men using acidic aniline blue stain and compare it with that of men with normal spermiogram. Discovering the relationship between sperm chromatin status and the semen parameters in infertile males. Finding the relationship between sperm chromatin status and ICSI outcome.This study included 60 infertile couples complaining from either primary or secondary infertility attended the clinics of infertility treatment center and underwent intracytoplasmic sperm injection.They were classified according to their spermiogram to normozoospermic group (N), whose female partner had bilateral tubal block, oligoasthenoteratozoospermic group (OAT) and finally, asthenoteratozoospermic group (AT). The female partners of the last two groups had normal reproductive function according to gynecological examination and laboratory investigations. All female partners included in this study were less than 35 years old to minimize the influence of advanced maternal age on ICSI outcome. Seminal fluid analysis were done for all and aniline blue stain was used in this study to evaluate sperm chromatin integrity. The main results of this study showed that infertile couples with impaired sperm parameters show high percentage of positive aniline blue stain i.e., sperm chromatin maturity defect and it is significantly higher than that in couples with normal spermiogram. The best cut off point of positive chromatin percentage associated with pregnancy was 40% i.e., when sperm chromatin abnormality exceeds 40% as detected by aniline blue staining method; the pregnancy rate start to regress. No significant differences in fertilization rate, cleavage rate, embryo quality and pregnancy rate were found among studied groups.No significant difference in ICSI outcome including pregnancy rate between couples with high percentage of sperm chromatin damage ?30% and those with low percentage of sperm chromatin damage < 30% were found. So the main conclusions are : Defective sperm parameters and chromatin immaturity are significantly associated with each other.Forty percent is the threshold of sperm chromatin immaturity after which ICSI outcome deteriorate and depending 30% as a threshold in this study revealed no significant difference in ICSI outcome among studied groups. Intracytoplasmic sperm injection has corrective and therapeutic role for defective sperm parameters and shows a unique capability to treat the most difficult cases of male infertility.

تقييم جهاز كتلة غاز الزفير كوسيلة لفحص وظائف الرئتين عند العراقيين الاصحاء == Evaluation of Expiratory Gas Mass Device As A Measure of Lung Function Test In Healthy Iraqi Subjects

Author name: نبراس حسين عبد الساده الحسناوي
Supervisor name: يسار محمد حسن الشماع | باسم متعب هادي زوين
General topic: Medicine
Specific topic: Physiology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: تعد فحوصات وظائف الرئتين من الوسائل المفيدة جدا لاغراض التشخيص والمتابعة والبحث العلمي للاصحاء ومرضى الجهاز التنفسي. وابسط تلك الفحوصات واكثرها معلومة هو الفحص السبايرومتري الذي يتضمن قياس السعة الحيوية القسرية (FVC) , وحجم الزفير القسري في الثانية الا | Lung function tests are very useful diagnostic, follow up and research measures for healthy and respiratory compromised subjects. The simplest, yet the most informative, lung function tests are the spirometric tests.They involve the forced vital capacity (FVC), the forced expiratory volume in first second (FEV1) and the forced expiratory volume ratio (FEV%). Several types of devices are used to perform spirometric tests based on the measurement of lung volumes and their subdivisions. Lung volumes, hence, are represented by the volume of expired air. There was a controversy about the difference between body and ambient temperatures which may extremely affect gas volume , but this is thought to be solved by temperature correction. Temperature correction, however, may not be the precise solution in extremely fluctuating environmental temperatures. A fixed amount of expired gas has a constant mass despite the changes in pressure or volume as is implied by the law of conservation of mass in a closed system by Antoine Lavoisier. The aim of this study is to construct and evaluate a simple device called expiratory gas mass device as a reliable, reproducible, accurate and convenient tool to substitute Vitalograph spirometer as a measure of lung function in healthy Iraqi subjects and testing the hypothesis that expired gas mass could significantly and acceptably alternate or conjugate its volume as measures of lung function. The design of the study is a cross sectional study. The statistically required number of participants was decided by a professional statistician to be 70 healthy subjects. However, 300 healthy subjects are included in the present research to cover the need for subgrouping (146 male and 154 female) aged 20 - 50 years (31.84 ± 9.28 years). The male and female were classified into three age groups for each one, ten years interval was considered to be suitable for classifying subjects.Subjects with abnormal lung function such as smokers were excluded. A uniform case sheet was assigned for every participant in the study which includes, name, gender, age, weight, height, smoking habits, residence, cultural status, socioeconomic status, present and past medical history, family history of respiratory illnesses. They were asked to perform the forced expiratory maneuver through the standardized spirometer and then through the newly constructed gas mass device.The accuracy (systematic error) and reproducibility (random error) were tested for the constructed gas mass device.A comparison between forced vital capacity (FVC) and adjusted gas mass (AGM) was made on two main groups , the male group and female group, both of them were subdivided to three age groups.The results revealed that there were no significant differences in values of FVC and AGM measured by standard Vitalograph spirometer and gas mass device respectively. Regression lines indicated that there were strong positive correlations between gas mass (GM) and FVC in male and female. Separate data for male and female are processed and the results indicated that FVC values and GM (or AGM) values are significantly higher in male than in female , P?0.05. Regression lines indicated that there was a negative correlation between age and both of FVC and GM, a positive correlation between height and both of FVC and GM and a weak positive correlation between weight and both of FVC and GM. From the present study, it can concluded that gas mass device is simple, portable and easily handled device. It is also reproducible and accurate device for the measurement of GM in normal subjects.

دور تخطيط العضلات احادي الليف على مرضى اعتلال الاعصاب المحيطية السكري وعلاقته بمؤشرات الاكسدة == The Role of Single Fiber Electromyography In Patients With Diabetic Neuropathy And Its Relation To Oxidative Stress Parameters

Author name: زاهد محمد علي كاظم
Supervisor name: احسان محمد عبود عجينة
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: الخلفية : يعتبر اعتلال الاعصاب المحيطية السكري من اكثر مضاعفات داء السكري شيوعا التي تؤثر على الاوعية الدموية الدقيقة. سريريا يؤثر المرض اولا على الاطراف القصيه مؤديا الى فقدان الاحساس في تلك الاطراف على نمط الجورب والقفاز. يؤثر المرض على كل من الالياف ال | Background : diabetic peripheral neuropathy (DPN) is the most common microvascular complication of diabetes mellitus (DM). Clinically, it affects the most distal extremities first resulting in a stock and glove pattern of sensory loss. It affects both small fibers (myelinated and un - myelinated) as well as the large myelinated nerve fibers. However, the earliest manifestations might be due to small fiber dysfunction. Hyperglycaemia and subsequent metabolic derangement is the main pathogenitic factor of DPN. However, oxidative stress, deficiency of neuroprotective factors and neurovascular insufficiency might play a role.AIMS : This study aims to : 1. Test the value of applying SFEMG in the diagnosis of diabetic peripheral neuropathy. 2. Determine the usefulness of SFEMG, compared to the standard NCS, in the early diagnosis of diabetic peripheral neuropathy. 3. Evaluate the correlation of the antioxidant/oxidant status in patients with and without diabetic peripheral neuropathy.Materials and methods : The study was conducted in the period from Nov. 2012 till Apr. 2014 and included 188 patients with type 2 DM that fall within age group of 45 - 65 years. Diabetics that suffer from clinical features of peripheral nerve dysfunction documented with abnormal electrophysiological findings were considered as patients with DPN (138 patients) and those who had no complaint of peripheral nerve dysfunction with normal electrophysiological study were considered as patients without DPN (50 patients). The study also included normal healthy persons who their age and gender matched that patients and considered as control subjects (50 persons). The patients and control enrolled in the study had undergone comprehensive assessment that included : clinical assessment (history and full examination), biochemical assessment (measurement of fasting blood sugar, glycated haemoglobin, total antioxidant capacity and advanced glycation end products) and electrophysiological assessment that included sensory and motor nerve conduction studies (NCS) with single fiber electromyography (SFEMG).Results : The study found that patients with DPN in comparison to patients without DPN and control subjects are older (p?0.05), had longer duration of DM (p?0.01), poorer glycemic control reflected by fasting blood sugar (p?0.01) and glycated haemoglobin (p?0.01), lower level of total antioxidant capacity (p?0.01) and higher advanced glycation end products level (p?0.01). Also, the study showed that the level of total antioxidant capacity and advanced glycation end products on one hand were correlated with the duration of the diabetes (p?0.01) and not correlated with glycated haemoglobin level on the other hand.The electrophysiological findings showed that patients with DPN had significant differences in NCS parameters when compared to patients without DPN and to control subjects in the form of axonal degeneration and demyelination with abnormal single fiber jitter and fiber density which were correlated with the duration of diabetes (p?0.01) but not correlated with glycemic control. Also the study demonstrated that SFEMG findings were correlated with NCS parameters (namely sural nerve) in patients with DPN. This study had concluded that the duration of diabetes, age, glycemic control and oxidative stress are important risk factors of DPN. Routine NCS is important mean for diagnosing DPN but it has limited sensitivity in the diagnosis of early stages of the disease and in assessing nerve degeneration and regeneration. Sensory nerves of lower limbs are helpful in discovering early stages of DPN when other tested nerves are normal. SFEMG examination in diabetic patients is important in showing the degree of re - innervation and nerve regeneration and in identification of subclinical nerve dysfunction.

دراسة العلاقة النسبية بين جريان الدم المخي - المشيمي والرحمي - المشيمي بالدوبلر للنساء الحوامل المصابات بداء السكري == A Study of Cerebro - Placental And Utero - Placental Ratio Doppler Velocimetry In Diabetic Pregnant Women

Author name: رياض وحيد العيساوي
Supervisor name: عقيل عبد المنعم حسن زوين
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: يعتبر داء السكري المصاحب للحمل هو احد مضاعفات الحمل الاكثر شيوعا والتي ترتبط بمعدلات هامة من الاعتلال والوفاة ما حول الولادة , ان العلاج الفعال لداء السكري ما قبل الحمل ولداء السكر المصاحب للحمل قد تبين تحسين النتائج والحد من وفيات ما حول الولادة بالمقارن | Diabetes mellitus complicating pregnancy is one of the most common antenatal complications that are associated with significant perinatal mortality and morbidity(Magee et al., 1993, Schmidt et al., 2001and Platt et al., 2002). Effective treatment of pre - existing as well as gestational diabetes mellitus was shown to improve outcome and reduce perinatal mortality, as compared to untreated patients (Lao TT, et al., 2001and Langer , et al., 2005). Doppler studies have conventionally been utilized for fetal surveillance. The use maternal uterine artery Doppler as a screening tool to predict subsequent development of pre - eclampsia and fetal growth restriction in later gestation have gained acceptance in recent years. Umbilical artery Doppler, middle cerebral artery Doppler have been widely used for the assessment of growth restricted fetuses. The role of Doppler study in diabetic pregnancies has not been unequivocally evaluated. An atherogenic factor, that promotes vascular endothelial growth, insulin - like growth factor - 1 has been implicated with micro vascular complication during pregnancy (Kathryn 2000). Presently, short - term beneficial metabolic effects of recombinant human insulin - like growth nfactor - 1 (rhIGF) - 1 therapy have been demonstrated in numerous diabetic conditions, including type 1 diabetes mellitus, type 2 diabetes mellitus. Until recently, insulin - like growth factor - 1, based upon its endothelial growth promotion, was considered a mediator of vascular disease( Bayes - genis et al., 2001a; 2001b). Conversely, increasing evidence indicate the protective mechanism of insulin - like growth factor - 1 against vascular derangements, relating to its ability of nitric oxide production (Okura et al., 2001). We sought to investigate the impact of insulin - like growth factor - 1 levels on maternal/fetal blood flow in diabetic non - hypertensive pregnant women; an avenue of research that has yet to be fully explored. Taken together, this information will materialize the intervention needed to prevent or at least ameliorate diabetes maternal and fetal outcomes. OBJECTIVES1 - To evaluate effect of diabetes mellitus on utero - and cerebro - placental Doppler velocimetry in pregnant women, such as resistive index (RI), pulsatility index (PI), Systolic/diastolic ratio (S/D)2 - To find whether a meaningful correlation exists between the above mentioned parameters and the levels of HBA1c and maternal IGF - 1.3 - To predict the most sensitive parameter detecting complications of diabetes mellitus on fetal outcomes. MATERIAL AND METHOD In this prospective cohort study a total of 190 pregnant women with single viable fetus, cephalic presentation, were enrolled after having their verbal and written consent and approval of the ethical committee at Faculty of Medicine. They were divided into two Groups : group (Ia) women with gestational diabetes mellitus (n=70), group ( Ib ) women with pre - gestational diabetes mellitus (n=64) and the remaining (n=56) participants (group II) were free from diabetes and hypertension and served as a control group. The study performed at Al Ameer Diagnostic Center in Al Najaf, between March, 2013 and August, 2014.Examination of the participants done twice, in the second and third trimesters. In each time, history and clinical examinations performed with Doppler ultrasonography of the pregnant women including interrogations of bilateral maternal uterine arteries, fetal umbilical and middle cerebral arteries in addition to measurement of IGF - 1 and HbA1c concentrations. RESULTS AND CONCLUSIONS 1 - IGF - 1 mean± SD in second trimester was 582.61± 183.7 ng/ml in group Ia, 429.5± 231.3 ng/ml in group 1b and 278.6 ± 60.8 ng/ml in the group II with significant difference among the groups (P=0.0001) In the third trimester the mean± SD was 653.69 ±197.6 ng/ml in group Ia, 487.5± 242.4 ng/ml in group Ib and 316.6 ± 60.9 ng/ml in group II with significant difference among the groups (P=0.0001).2 - HbA1c mean± SD in second trimester was 6.59± 0.89 % in group Ia, 6.42 ± 0.9% in group Ib and 5.35 ± 0.56 % in group II with significant difference between group Ia and group II, as well as between Ib and group II, but no significant difference between Ia and Ib groups.Third trimester HbA1c mean value was 6.29 ± 0.78 % in group Ia , in group Ib 6.27 ± 0.87 % and 5.08 ± 0.57% in group II.There was no significant difference between group Ia and Ib (P=0.843) , while there was a significant difference between group Ia and group II as well as between group Ib and group II(P=0.0001).3 - Uterine artery Doppler velocimetry was considered more sensitive predictor than other arteries in evaluation of fetal outcomes and its sensitivity in the total studied population (190) was 44.3%, in gestational diabetic pregnancy group was 58.3%, in pregestational diabetic pregnancy 34.3% and in control was 29.4%.4 - Multiple maternal and fetal arterial Doppler studies at same time yield high predictive values in evaluation of fetal outcomes with general sensitivity in the total studied population was 63.6%, specificity 79.4%79, PPV 72.2% and NPV 71.7%. In gestational diabetic pregnancy the sensitivity was 94.4%, specificity was 61.8%, PPV was 72.3%, NPV was 91.3%. In pregestational diabetic pregnancy sensitivity was 88.6%, specificity was 86.2 %, PPV was 88.6 %, NPV was 86.2%. In control healthy pregnancies the sensitivity was 70.6 %, specificity was 71.8%, PPV was 52.2%, NPV was 84.8 %.

العلاقة بين اصناف الدم المختلفة وعوامل الخطورة الرئيسية لامراض الشرايين التاجية == The Relationship Between Blood Groups And Major Cardiovascular Risk Factors of Coronary Artery Diseases

Author name: رواء هادي شريف
Supervisor name: سامي رحيم الكاتب | احمد نعمة رجيب
General topic: Medicine
Specific topic: Physiology
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: امراض الشرايين التاجية السبب الرئيسي لمعظم حالات العجز والوفاة في الدول المتقدمة. العمر, الجنس, السمنة, اختلال مستويات الدهون, التدخين, ارتفاع ضغط الدم, مرض السكر, الالتهابات, والاكسدة الضارة من اهم عوامل الخطورة الرئيسية للمرض. مجموعة من الدراسات اوحت ب | Coronary artery diseases are the most important causes of mortality and morbidity in many developing countries. Age, gender, obesity , lipid disturbance, smoking, hypertension, diabetes mellitus, inflammations and oxidative stress are considered as major risk factors of disease. Many of studies suggest that there is association between blood groups and risk factors of coronary artery diseases.Objective : The aims of study are to find if there is an association between blood groups and CADs and to study some of the causes that may be lead to this association. Also, it aims to determine the type of relation between some important factors such as, C - reactive protein (CRP), advanced glycation end products(AGEPs), and glutathione peroxidase - 3 (GPX - 3) with blood groups and coronary artery diseases. Materials & Methods : Ninety persons included in the study (60 as patients and 30 as healthy controls). Where the patients suffering from CAD, the controls are free from this diseases. Their ages ranged between 25 - 70 years old and they were divided into four groups according to the blood groups (A, B, AB, and O). This study is based on case control study done in Al - Sadder Teaching hospital (Cardiac Center) in Al - Najaf Al - Ashraf city from December 2012 - to October 2013. Some information were taken from each person in the study like, name, age, sex, address, weight and height. Finally and after getting permissions, the blood group was analyzed for each person in the study, then the sera were collected from those 90 persons and analyzed by using enzyme linked immunosorbent assay (ELISA) for CRP, AGEPs, and GPX - 3, meanwhile for lipids assay we used spectrophotometer. Results : The results of the study show that the blood group O( 33.7%) predominates in both studied groups (Patients and controls) followed by A (29.6%), B (27.4%), and AB (9%). There is no significant differences in the percentage of blood groups between patients and controls groups (P >0.05). Also, the results of the study show that the incidence of disease increase with age increases and in male (80%) more than female (20%). Also, the results show that only patients group has obese individuals and most obese were from blood group B(80%), followed by A(66.6%), O(60.8%), and AB(50%). The statistical analysis of the study shows that blood group AB among patients and blood group B among controls have a significant increasing (P<0.05) in the serum level of total cholesterol, and C - reactive protein (CRP). While, the results show that blood groups (B&AB) of patients have a significant increasing in the serum level of triglycerides. The blood group A among patients and blood group B among control show a significant increasing in the LDL cholesterol serum level, while blood groups (A& B) of patients have a significant reduction in the serum level of HDL cholesterol. Also, the study shows that blood group A of both patients and controls has a significant increasing in the serum level of advanced glycation end products (AGEPs), while the blood groups (A& B) of patients have a significant decreasing in the serum level of glutathione peroxidase - 3 (GPX3). Finally, the results of the study show a negative correlation (R= - ) between AGEPs and GPX3 in blood groups ( B&O) of both patients and controls group.Conclusions : Results of the present study show that the blood group O predominates in the studied groups in Al - Najaf Al - Ashraf city followed by A, B, and the last is AB. The incidence of CAD 2 to 4 time more common in male than female and incidence of CAD increase with age increases in both sex. The incidence of CAD 2 to 4 time more common in male than female and incidence of CAD increase with age increases in both sex. Also, There is a high percentage of subjects from blood groups (A, B, and AB) suffering from disturbance in the serum level of lipids and high level of CRP, which they were associated with atherosclerosis disease that was considered one of the most important leading cause of CAD.

توازن الحركيات الخلوية وعلاقة اليللات التطابق النسجي في كل من النوع الاول لمرضى السكري واشقائهم في دراسة مقارنة == Cytokine Balance (Th1/Th2) And Hla - Dr Association In Type I Diabetic Patients And Their Siblings In A Comparative Study A Thesis

Author name: محمد علي سلمان علیوي الكعبي
Supervisor name: كریم ثامر مشكور الكعبي | جعفر كاظم الموسوي
General topic: Medicine
Specific topic: Microbiology - Immunology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: It has been proposed that the balance of Th1 and Th2 cells is critical in the development of Type 1 diabetes and much of the genetic susceptibility to IDDM maps to the MHC class II region, thus one approach to investigate the immunopathogenesis of diabetes is to study first degree relatives of affected individuals.A case - control study was performed on 120 persons, they divided into three group which are diabetic, siblings and control (40 persons in each group), who attended to Al Zahraa Teaching Hospital /Diabetic Center in Al Kut between the period from April; 2012 till April; 2013. OGTT performed on both diabetic siblings and control, results have showed that the means of OGTT is highly Significant (P value = 0.000) in both siblings and control groups, whereas there were no Significant differences in the Means of differences of OGTT in both tested groups (Siblings and Control), P value = 0.288, although the mean of differences of OGTT is higher in siblings group (25.3250) than that in control group (22.0750). Cytokines levels (IFN - gamma, IL - 2, IL - 4 and IL - 10) were determined among the study groups (type 1 diabetes, siblings and control) by ELISA method, results showed that the means of IFN - gamma and IL - 2 levels were highly Significant (P value = 0.012, 0.037 respectively) in type 1 diabetic group , in comparison to their corresponds among both siblings and control groups, whereas the mean of IL - 4 levels was non - significant (P value = 0.738) among the study groups (type 1 diabetes, siblings and control), in spite it was higher in control group (0.81936) than that in both type 1 diabetic patients and their sibling (0.74387, 0.77593 respectively).While in case of IL - 10 results showed that the mean was highly significant among control group (P value = 0.030) in comparison to its corresponds among both type 1 diabetic patient and their siblings. Distribution of HLA - DRB1 loci among the study groups (type 1 diabetic patients, siblings and control group) were performed using MR.SPOT ROBOTING system, results showed that the frequencies of HLA - DRB1*3,*4 {25 (69.4%)} among diabetic group were Significant in differences (P value = 0.002), in comparison to the corresponding frequencies among control group {11 (30.6%)}, in comparison to the frequencies of other HLA - DRB1 loci in both diabetic group and control group { 15 (34.1%), 29 (65.9%)} respectively.Meanwhile results showed that there were no Significant differences (P value = 0.116), in the frequencies of HLA - DRB1*3,*4 {25 (58.1%)} among diabetic group in comparison to the frequencies of HLA - DRB1*3,*4 {18 (41.9%)} among siblings group, in compare to the frequencies of other HLA - DRB1 loci among diabetic group and sibling group {15 (40.5%), 22 (59.5%)} respectively, also results have showed that there were no Significant (P value = 0.104), in the frequencies of HLA - DRB1*3,*4 {18 (62.1%)} among sibling group in compare to the frequencies of HLA - DRB1*3,*4 {11 (37.9%)} among control group, in compare to the frequencies of other HLA - DRB1 loci among sibling group and control group {22 (43.1%), 29 (56.9%)} respectively. In another part of this study we estimate the relation between means of HbA1c and their corresponding HLADRB1 Loci among the study groups, result have showed that there was a significant relation (P value = 0.010) between the Means of HbA1c and their corresponding HLA - DRB1*03 Locus among the study groups. In conclusion the higher Mean of differences of OGTT among Siblings group might reflect the potential of them to develop diabetes, also T1D is Th1 mediated disorder, while Th2 cytokines profile might play a protective role against the development of T1D,in addition the genetic predisposition of diabetic Siblings for development of diabetes since, both diabetic group and sibling group showing the highest frequencies of HLA - DRB1 *3,*4, in compare to Control group and finally HbA1c might have an inheriting pattern. In conclusion diabetic siblings more prone for development of diabetes in future.

تقييم حالة الحديد، فيتامين ج والهرمونات في انخفاض الخصوبة لدى النساء == Assessment of Iron Status, Vitamin C And Hormones In Subfertile Women

Author name: ميسم محمد حسين الكعبي
Supervisor name: سامي رحيم الكاتب
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: انخفاض الخصوبة مشكلة شائعة ولكن علاجها في بعض الاحيان غير كافي لعدم تشخيص الاسباب بالكامل. الحديد عنصر ناقل للاوكسجين ولذلك فهو محفز لحيوية كل الخلايا والانسجة في الجسم. نقص الحديد من اكثر مشاكل نقص الغذاء الشائعة في العالم. لذلك ربط الحديد مع الخصوبة يح | Subfertility is a common problem, but the treatment is sometimes inadequate because the causes are not fully diagnosed. Iron is essential for oxygen transport and therefore promotes health of all cells and tissues. Iron deficiency is the most common nutritional deficiency in the world. Therefore, the connection between iron and fertility need more explanation. Some researchers have suggested that iron may be important for ovulation as the egg has an increased demand for iron while it matures and women who do not get sufficient amounts of iron may suffer anovulation and possibly poor egg health, which can inhibit pregnancy at a rate 60% higher than those with sufficient iron stores in their blood. However women planning to become pregnant should test for serum ferritin level and accordingly if low they can use iron supplements because they may help them to prevent iron deficiency and also improve fertility. Vitamin C is essential for the uptake and absorption of iron. The objective of this study was to assess the iron status, vitamin C and some hormone levels in subfertile women. The sample of study consists of 106 women subdivided into two groups : first consist of 76 subfertile women were taken from the outpatient to fertility center in the gynecological and obstetrical teaching hospital in Kerbala city and from multiple gynecology/obstetrics privet clinics. Second consist of 30 fertile women (control and comparative) who obtained from outpatients and some relatives. The subfertile women subdivided into two subgroups according to the cause of subfertility in presented study : first group : ovarian dysfunction (OD) either due to polycystic ovary syndrom (PCOS) or due to other cause (OC) like anovulation, hyperprolactenemia, premature ovarian failure. Second group : unexplained (idiopathic). Also the subfertile women subdivided into two subgroups according to the type of subfertility : primary and secondary. All of them were at reproductive age (16 - 45) years and there BMI ranged between (20 - 45 kg/m2). The study was achieved throughout a period which extends from July 2012 - October 2013. The protocol of study summarized as following : At day 3±1of menstrual cycle (menstrual phase), all women underwent the measurement of concentrations for multiple hormones in the blood like luteinizing hormone, follicle stimulating hormone, estradiole, prolactine and Testosterone. In addition to iron status (serum iron, serum ferrtin and total iron binding capacity) and vitamin C also assessed. And at day 13 of menstrual cycle (near time of ovulation), all women underwent two investigations : first : the measurement of concentrations of luteinizing hormone, serum Leptin and antioxidants as vitamin C and glutathione. In addition to Iron status (serum iron, serum ferrtin and total iron binding capacity) and hemoglobin concentration also estimated. Second : estimation the size and the number of dominant follicles in both ovaries by ultrasonograghy. While at day 23±1 of menstrual cycle (secretary phase), all women underwent two investigations : first : estimation the concentration of serum progesterone to detect ovulation. Second : estimation the endometrial thickness by ultrasonograghy. The results of study shows : • A significant increase (P<0.05) in number of subfertile women with age group (16 - 25) years and there were highly significant decreases (P<0.001) in number of women with age 36 - 45 years for both primary and secondary subfertility. While no significant difference (p>0.05) in age group (26 - 35) years.• A significant increases (P<0.05) in body mass index for subfertile women due to polycystic ovary syndrome. Also a significant increase (P<0.05) in serum leptin for women with overweight and obese as compared with normal weight.• A significant increase (P<0.05) in serum ferritin level for polycystic ovary syndrome at day 13 of menstrual cycle. Also, a significant decreases (P<0.05) in number of polycystic ovary syndrome in those having serum ferritin lower than normal at day 13 of menstrual cycle.• A significant increase (P<0.05) in the number of subfertile women due to ovarian dysfunction other than polycystic ovary syndrome who having a lower serum level of vitamin C at day 3 of menstrual cycle. • A significant increase (P<0.05) in serum testosterone level in polycystic ovary syndrome group at day 3 of menstrual cycle. • A significant increase (P<0.05) in serum prolactine level in group of ovarian dysfunction due to other cause at day 3 of menstrual cycle. when serum ferritin level lower than or within normal value. • A significant decrease (P<0.05) in serum level of luteinizing hormone for group of ovarian dysfunction due to other cause at day 13 of menstrual cycle when serum ferritin less than normal but no significant differences (P>0.05) in serum luteinizing hormone when serum ferritin within normal value. • A significant decrease (P<0.05) in serum luteinizing hormone for group of ovarian dysfunction due to O.C when vitamin C within normal value at day 13 of menstrual cycle.• A highly significant decrease (p<0.001) in endometrial thickness for all subfertile group at day 23 of menstrual cycle.• No significant difference (P>0.05) in serum levels of follicle stimulating hormone, estradiole and glutathione as compared with other parameters in fertile and subfertile women.It was concluded that the decrease in serum ferritin and vitamin C levels in the blood may be associated directly or indirectly with subfertility caused by ovarian dysfunction.

تاثير التلميزارتان والاجسام المضادة لمستقبلات الانجيوتنسين نوع 1 في انقاص الارواء الدموي واعادته لعضلة القلب وموت الخلايا المبرمج في ذكور الفئران == Effects of Telmisartan And Angiotensin II Type1 Receptor Antibody In Myocardial Ischemia/Reperfusion Injury And Apoptosis In Male Mice

Author name: سعاد تريجي زامل العكيلي
Supervisor name: نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: يمثل نقص ارواء عضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. الانجيوتنسين الثاني قد يساهم في الاصابة بسبب اعادة الارواء عن طريق زيادة الاكسدة والعوامل الالتهابية. الانجيوتنسين الثاني يمارس معظم ا | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem following thrombolysis, angioplasty and coronary bypass surgery. Angiotensin II may contribute to reperfusion injury by increasing oxidative stress and inflammatory factors. Ang II exerts most of its effects via AT1Rs. Objective : This study was undertaken to investigate the potential role of Telmisartan and AT1 - AB in amelioration of myocardial I/R injury induced by ligation of coronary artery in mouse model. Materials & method : Adult male Swiss - albino mice were randomized into 6 equal groups. Group (1) sham group : Mice underwent the same anesthetic and surgical procedure as the active control group except ligation of LAD coronary artery.Group( 2) active control group : Mice were subjected to regional ischemia for 30 min by ligation of LAD coronary artery and reperfusion for 2 hours.Group( 3) control vehicle group (1) : Mice in this group injected with DMSO (vehicle for Telmisartan ) via IP route & underwent Myocardial ischemia for 30 minutes by ligation of (LAD) coronary artery & reperfusion for 2 hr. Group( 4) control vehicle group (2) : Mice injected with D.W ( vehicle for AT1 - AB) via IV route & underwent Myocardial ischemia for 30 minutes by ligation of (LAD) coronary artery & reperfusion fore 2 hr. Group (5)Telmisartan treated group : Mice pretreated with Telmisartan 0.5mg/kg i.p 30 minutes before ligation of LAD coronary artery. Group(6) AT 1 - AB treated group : Mice pretreated with AT 1 - AB (1Mcg/gm.) of body weight via IV route 30 minutes before ligation of LAD coronary artery. Results : Compared with the sham group, Levels of TNF - ? & IL - 1?, IL - 6,caspase 3 and plasma level of cardiac troponin I increased in control group (p<0.001).Levels of Bcl2 decreased in control group(p<0.001). Histologically ,All mice in control group showed a significant (p<0.001) cardiac injury. Both Telmisartan and AT1 receptor antibody significantly counteract the increase in myocardium level of TNF - ?, IL - 1B,IL - 6,caspase 3 ,plasma cTnI (P < 0.001). Furthermore, the Telmisartan and AT1 receptor antibody significantly increased in myocardium level of Bcl2. Histological analysis revealed that both Telmisartan and AT1 receptor antibody markedly reduced (P < 0.001) the severity of cardiac injury in the mice underwent LAD ligation procedure. Conclusion : The results of the present study reveal that Telmisartan and AT1 receptor antibody ameliorate myocardial I/R injury in Mice via interfering with inflammatory reactions & apoptosis which induced by I/R injury.

تغيرات الرسم الكهربائي للدماغ عند الحوامل في مرحلة ما قبل التشنج الحملي وعلاقتها ببعض المؤشرات الحيوية الالتهابية == Electroencephalographic Changes In Preeclamptic Females And Their Relation With Some Inflammatory Biomarkers

Author name: مهند يحيى ادريس المحنه
Supervisor name: احسان محمد عبود عجينة
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: يسجل الرسم الكهربائي للدماغ الفعالية الكهربائية الذاتية المتولدة في قشرة الدماغ. اشارة الرسم الكهربائي للدماغ هي قياس للتيارات المتحركة في قشرة الدماغ. عندما تحفز خلايا الدماغ (العصيبات), يتم توليد التيار الارتباطي في التشجرات العصبية للخلايا. يولد هذا ال | Electroencephalography (EEG) records the spontaneous electrical activity generated in the cerebral cortex. When brain cells (neurons) are active, synaptic currents are produced within their dendrites and this current generates an electrical field over the scalp. The EEG waves measure this current that flow during synaptic excitations of pyramidal neurons' dendrites. Preeclampsia is a disease of pregnancy characterized by a blood pressure of 140/90 mmHg or more on two separate occasions after the 20th gestational week in a previously normotensive woman. This is accompanied by significant proteinuria (>300 mg in 24 hours) and is associated with endothelial cell damage. The markers of endothelial activation or inflammation have been blamed to play an active role in preeclampsia (Sibai et al., 2005). This study included 97 pregnant with a confirmed diagnosis of preeclampsia as a patient group with a gestational age ranging between 20 - 38 weeks. They are divided into three subgroups according to their gestational age (20 - 26 wk, 27 - 32 wk and 33 - 38 wk) and the mean of their age was 29.34±4.82, 30.14±5.33 and 32.54±6.22 years respectively. In addition, 72 women with normal pregnancy have been included as control group. These were also subdivided into three subgroups just like that of the patients and their age matches them too. Participants belong to both patient and control groups had underwent same procedure, examinations and tests like EEG and serological tests for Intracellular Adhesion Molecule 1 (ICAM I), interleukin - 6 (IL6) and C - reactive protein (CRP), in parallel. Results showed that there were statistically significant increased levels (P < 0.05) of ICAMI, IL - 6 and CRP values between patient and control groups and between the patient with mild and those with severe preeclampsia in all tested gestational age subgroups. This supports the hypothesis stating a role of cytokines and inflammatory biomarkers in the mechanism underlying preeclampsia. Moreover, and at all gestational age subgroups, the ICAMI, CRP and IL - 6 values were higher in patients with severe disease when compared with those having milkd disease. This strengthens more the relation of these biomarkers with the progression of preeclampsia. There were abnormal EEG changes in about 52 % of the patients with preeclampsia and these changes were reported in all gestational age subgroups and were in the form of spikes, polyspikes, slow sharp waves. This shows that preeclamptic females are prone to some excessive brain activities that may put them in danger of abnormal brain function. Furthermore, these EEG abnormalities were related to the severity of the preeclampsia as they were recorded in severe cases more and at all gestational age subgroups. Also there were statistically significant elevation regarding ICAMI (at the second and third gestational age subgroups), IL - 6 values (at the second gestational age subgroup) and CRP (at all gestational age subgroups) among patients with and without abnormal EEG changes. This suggests a strong relation between these inflammatory biomarkers with the occurrence of abnormal EEG changes in preeclamptic females. In conclusion, an inflammatory process is blamed to be the underlying mechanism of preeclampsia as proved by the elevated levels biomarkers in these patients. Both the abnormally elevated biomarkers and the abnormal EEG changes occur in preeclamptic patient in different gestational ages above 20 weeks and occur in different disease severity.

التشخيص المقارن المصلي والجزئي لداء المقوسات وبعض الاصابات الفيروسية لبعض النساء الحوامل اللوات يعانينن من الاجهاض في محافظة النجف الاشرف == Serological And Molecular Comparative Diagnosis of Toxoplasmosis And Certain Types of Viral Infections (Torch) In Aborted Women In Al - Najaf Province

Author name: ذكرى عبد الله محمود المياحي
Supervisor name: باقر عبيس سلطان | صباح نعمة محمد الفتلاوي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: اجريت هذه الدراسة في محافظه النجف الاشرف / العراق، وتضمنت جمع عينات من دم النساء الحوامل اللواتي يعانين من الاجهاض اول مرة او متكررة وكان عدد الحالات 57 حالة. اعمارهن يتراوح بين 15سنة الى40 سنة واللواتي يراجعن مستشفى الزهراء التعليمي والمختبر المركزي وبع | Toxoplasmosis and viral infections such as Rubella , Cytomegalovirus and Herpes virus (TORCH) are feared risk during pregnancy. The rate of toxoplasmosis and of subclinical infected infants at birth with the risk of late manifestation is still unclear, whereas such data are fairly well - known for Rubella , Cytomegalovirus and Herpes virus. The respective major diagnostic issues in pregnancy, the laboratory diagnosis, and its rational use in combination with clinical information are presented , also the value of passive prophylaxis, therapy, and prenatal diagnosis as well as the possible management for diminishing the infection problems in pregnancy.Aims of study : The study was aimed to evaluate the ELISA result for Toxoplasma, rubella, cytomegalovirus (CMV) and Herpes virus (TORCH) IgM and IgG and compare the results of the ELISA with RT - PCR to arbitrate discordant results.Methodology : The present study was carried out on 57 pregnant women, all of them had with bad obstetric history (BOH) of abortion twice or more. Patients were between 15 - 40 years of age, and they were attending Al - Zahraa Maternity and pediatric Teaching Hospital, Al - Hakeem Hospital and some private clinics, in AL - Najaf province during the period from May 2012 till May 2013. Serological evaluation for TORCH infections was carried out by using IgM and IgG Enzyme Linked Immunosorbent Assay (ELISA ) method and molecular technique ( DNA; RNA - RT - PCR). Each blood sample was divided into two part , one part plane tube for separation of serum that were screened for the presence IgM and IgG antibodies against Toxoplasma gondii ,Cytomegalovirus Rubella virus and Herpes simplex virus by using (ELISA test ).The tests were performed according to manufactures instruction.The other part (blood )EDTA tube was used for DNA 0f detection Toxoplasma , CMV and Herpes simplex virus , while RNA extraction of Rubella virus.The blood and serum sample were stored in refrigerator (2 - 8C?) before the analysis.The extracted DNA and RNA were stored at - 20C? till used within 24 hours. All ELISA Results samples were compared with Real - Time PCR result. Statistical analysis : Statistical program SPSS version (20.0) was used to study the significant difference between tests in each type of antibody (IgM and IgG). (X2 - test) was used after detection of normal distribution to the data and appropriate P<0.001 consider significant. Results : Both IgM / IgG was Seropositive to T. Gondii , Rubella , CMV and HSV - 2 which were 49.12/80.70%, 21.05/49.12% , 45.61/70.18% and 1.21/36.20% respectively. Samples were seropositive to IgM and results were retested by RT - PCR method. Maximum number of IgM /IgG seropositive cases of aborted women was 19(32.75%)/57 that associated with Toxoplasma ,Rubella and CMV infection by RT - PCR ( 10(17.54%), 6( 10.53%) and 3(5.26%) respectively ). The ELISA TORCH IgM and IgG assays provided results comparable to RT - PCR indicated significant differences among Toxoplasma and viral infection. The ELISA TORCH infection IgM and IgG assays showed a lower specificity with for Toxoplasma IgM (p=0.001), poor for CMV IgM and moderate for Rubella virus IgM.Conclusion : The accurate diagnosis of TORCH infection should be done by molecular method.

تاثير التعويض الجزئي والكلي للاسنان على فحوصات كفاءة الرئتين عند مرضى السكري نوع - 2 == Effect of Partial And Complete Denture Treatment On Spirometric Tests In Type 2 Diabetic Patients

Author name: حسين مهدي حسين الخفاجي
Supervisor name: يسار محمد حسن الشماع
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: في هذه الدراسة العرضية, تم اختيار 140 شخصا بصورة عشوائية, من بينهم 90 كان مصابا بالسكري والباقون كانوا سليمين ,لدراسة تاثير ارتداء طقم الاسنان الكلي والجزئي من قبل مرضى السكري على فحوصات كفاءة الرئتين وبالتحديد, , السعة الحيوية القسرية (FVC)و الحجم الزفير | In this cross sectional study, a total number of 140 subjects selected randomly, 90 of them were diabetic and the remaining were normal, to study the effect of complete and partial dentures wearing by diabetic patients on pulmonary function test particularly peak expiratory flow rate (PEFR), forced expiratory volume in the first second (FEV1), forced expiratory volume ratio (FEV1/FVC) ratio (FEV1%), forced vital capacity (FVC), and also the retropharyngeal space (RPS), the level of Endothelial selectin (E - selectin) and the level of Vascular Cell Adhesion Molecule - 1(VCAM - 1)was also compared, in this study, between the diabetic and the normal subjects.The results indicate that the spirolab III was accurate and reproducible and can be used in all related studies.The effect of wearing of complete and partial dentures by diabetic patients on FVC, FEV1, PEFR and also the degree of alteration of the RPS were measured.1) The results of the study shows a significant increase in values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) (p<0.05), while there was no significant change in the value of FVC after the replacement of complete denture.2) No statistically significant differences in the values of FVC, FEV1, FEV1%, PEFR and retropharyngeal space (RPS) was observed after replacement of partial denture without free end extension3) There was a significant increase in values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) (p<0.05), never the less there was no significant change in the value of FVC after the replacement partial denture with unilateral free end extension.4) There was a significant increase in values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) (p<0.05), However there was no significant change in the value of FVC after the replacement partial denture with bilateral free end extension5) The results showed that in poor and good control diabetic patients there was a significant decrease in the values of FEV1, PEFR, FEV1%and RPS after the removal of all types of dentures(p<0.05), while no significant change in FVC.6) Statistical significant increase in Serum E - selectin and VCAM - 1 level was found in diabetic patients when compared to that of the normal subjects (p<0.05).7) No statistical significant difference between serum E - selectin and VCAM - 1 level in diabetic patients after and before denture placement (p>0.05). The cause of the significant change in the values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) in case of complete and partial denture with free end extension replacement may be due to pressing of the retropharyngeal space bilaterally and unilaterally by the complete and partial denture and the FVC was not significantly changed because it may not be affected by denture support, while the elevated Serum E - selectin and VCAM - 1 level might indicate endothelial activation and inflammation.In conclusion we can conclude that 1. The spirolabIII used in this study is an accurate and reproducible device and can be used in the assessment of lung function test.2. Values of FEV1, FEV1%, PEFR and retropharyngeal space (RPS) where significantly higher after the replacement of complete denture and partial denture with bilateral and unilateral free end extension in diabetic patients (p<0.05), while there was no significant change in the value of FVC.3. No statistically significant differences in the values of FVC, FEV1, FEV1%, PEFR and retropharyngeal space (RPS) was observed after partial denture without free end extension has been replaced in diabetic patients.6. In poor and good control diabetic patients there was no significant change in the value of FVC, while the values of FEV1, PEFR, FEV1%and RPS where significantly decrease after the removal of all types of dentures.8. Statistical significant increase in Serum E - selectin and VCAM - 1 level was found in diabetic patients when compared to that of the normal subjects.9. The results for serum level of E - selectin and VCAM - 1 shows no significant difference diabetic patients after the placement of all types of dentures (p>0.05).

تعقب الليشمانيا الجلدية بالاختبارات التحليلية الوبائية والطفيلية والجزيئية والكيمياحياتية == Tracking of Cutaneous Leishmaniasis By Epidemiological, Parasitological, Molecular And Biochemical Analysis

Author name: سندس نصیف الحجیمي
Supervisor name: باقر عبیس سلطان | محسن عبد الحسین الظالمي
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: The present study was performed to identify the species and strain of Leishmania parasite isolated from different endemic areas. It was carried out on cutaneous leishmaniasis (CL) in five Iraqi provinces as following : Al - Najaf province ( Al - Hakeem and Al - Sadder teaching hospitals), Babylon province ( Marjan hospital ), Al - Qadisya province (AL - Sadder Teaching hospital ), Karbala province (Al - Hussain General Hospital) and Kut province (Al - Zehraa Teaching and Al - Kerama Hospitals). It covered seven hospitals as a field of investigations from October 2010 to December 2012.Cellulose acetate electrophoresis has been performed in Walter Reed Institute of researches in USA. A total of 126 cases comprising 48(38%) females and 78(62%) males were studied. The highest infected age group was 21 - 30 years with a rate of 27.8% and the lowest rate was 11.9% at the age group of 10 and less years.Geographical distribution of CL among hospital patients indicated that rural areas were with highest rate (54%) while in urban areas were with(46%).The number of ulcers per one patient differs for each patient. The highest rate of multiple lesions was 73% in comparison with single lesion (27%).The type of infection showed that disease was in wet type (82.5%) more than in dry type (17.5%). Direct smears were made by lesion aspiration,skin scraping and discharged blood methods , by which 90 (71%) patients gave positive result under light microscope.Modified NNN medium and RPMI - 1640 with fetal bovine serum followed by sub - culture in drosophila Schniders media were performed for each case. Out of 126 cases,83(65%) gave positive growth. Further characterization of the causative parasite species and strain made by molecular and biochemical techniques.Out of 83 postive growth culture,only 52 specimens were studied by nested - PCR, using kinetoplast minicircle fraction amplification to detecte the species of parasite.It was found that 45(86.5%) cases in the generation of a 560 bp DNA and 7(13.4%)patients displayed a fragment of 750 bp, corresponding to L. major and L. tropica, respectively.This test revealed that L. tropica and L. major are the causative agents of infection with dominant shifting to the L. major.Cellulose acetate electrophoresis (CAE) was performed for 20 mass cultivated cultures. Isoenzyme profiles of these isolates were compared with reference strains of Leishmania spp. using cellulose acetate electrophoresis and 4 enzyme systems (glucose phosphateisomerase,leucil phosphate,manose phosphate isomerase and 6phosphogluconate dehydrogenase). The results showed that L.major(LV39) isolate were in 20 mass cultivated culture.Most of the CL patients in Najaf province(2011,2012) were from Haidariya in north district. High prevalence of disease was observed in Autumn and Winter.The highest number of cases was recorded during February and December.

مقارنة بين النوع الثاني من مرض السكري ومرض السكري المناعي الذاتي المتاخر الظهور للبالغين باستخدام مؤشرات الخلايا اللمفاوية وملف الحركيات الخلوية == Comparison Between Type 2 Diabetes Mellitus And Latent Autoimmune Diabetes of Adults Using Lymphocytes Markers And Cytokines Profile

Author name: سوسن محمد جبار الحسناوي
Supervisor name: سلمان عزيز الجبوري
General topic: Medicine
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: This study was done to investigate whether Type 2 diabetes and Latent autoimmune diabetes of adult (LADA) patients have similar or different immunological profile.Therefore, measurement of serum levels of adhesion molecules (Serum intracellular cell adhesion molecule(sICAM - 1), Serum vascular cell adhesion molecule (sVCAM - 1) and Serum endothelial Selectin (sESelectin)), chemokines (CCL2, CCL3 and CCL4) and cytokines (IL - 6, IL - 1receptor antagonist and IFN - ?) in these patients was done. In addition to the influence of BMI, gender, age and diabetes duration on these systemic immune mediators were analyzed. Also, the relationships of the in vivo levelsof these mediators with micro - & macro - vascular complications in type 2 diabetes & LADA were studied.Three hundred individual were enrolled in this study and were divided into two subgroups : patients and control groups (280 patients& 20 control). From the 280 patients group who were clinically diagnosed as type 2 D.M., 34 patients were GADA positive and considered as LADA cases. Serum samples were taken from The 34 LADA cases, 36 cases of D.M.2 (selected randomly from the remaining 246 diabetic type 2 patients) & 20 healthy control subjects.The prevalence of LADA among D.M.2 patients was 12.1%. A significant difference in family history, BMI, FBS, HA1C & duration of D.M. was found in D.M.2 cases compared to LADA cases. The mean sVCAM - 1level of control group was significantly lower than D.M.2 and LADA group, P<0.05. The sE - Selectin level was higher in LADA group than control and D.M. type 2 group & significantly different when compared to control group (P<0.05). The sVCAM - 1 level had a significant positive correlation (R=0.25, P=0.017) with BMI in all groups. LADA group had significantly higher values of CCL2 while D.M. type 2 had the least values (P=0.002). The mean IL - 6 of D.M. type 2 group was (11.6±3.1), for LADA group it was (12.4±1.7) and for the controls it was 0.95 ± 0.14. There was a statically significant difference between D.M.2 vs. controls, and LADA vs. controls (P<0.05), regarding the mean IFN - ?, it was significantly higher in LADA group than D.M.2 group (87.5 ± 20.3) vs. (40.3 ± 4.5) respectively, (P=0.032).The mean IL - 1receptor antagonist was significantly higher in D.M. type 2 and LADA cases than controls (P<0.05). A significant positivecorrelation between IL - 6 and sICAM - 1, (R= 0.27, P= 0.013) & a highly significant positive correlation between IL - 6 and IL - 1RA, (R= 0.35, P=0.001) were found. IL - 6 & IL - 1RA serum levels in D.M. type 2 patientswere significantly associated with the complications where p - value?0.05. For conclusions, LADA is clinically underestimated among D.M. type 2 patients and increases with increasing age. Also, Levels of CCL2 and IFN - ? are associated with autoimmunity, Levels of adhesion molecules & chemokines are associated with complications of diabetes.

تاثير الايتانرسبت على السايتوكينات وموت الخلايا المبرمج جراء انقاص الارواء الدموي واعادته لعضلة القلب في ذكور الفاران == Effect of Etanercept Against Myocardial Ischemia/Reperfusion Injury In Male Mice

Author name: سيف محمد حسن
Supervisor name: نجاح رايش هادي الموسوي | فاضل غالي يوسف العمران
General topic: Medicine
Specific topic: Medicines and Toxins
Degree: Master
Language: English
University location: Najaf
First pages:
Abstract: يمثل نقص ارواءعضلة القلب واعادة الارواء مشكلة ذات صلة سريريه مرتبطة بالجلطات والقسطرة وجراحة تغيير الشرايين التاجيه. تشمل اصابة عضلة القلب بسبب نقص التروية الدمويه واعادتها ضعف مقلص القلب، عدم انتظام ضربات القلب وكذلك تلف الخلايا العضلية التي لا رجعة في | Background : Myocardial ischemia - reperfusion represents a clinically relevant problem associated with thrombolysis, angioplasty and coronary bypass surgery. Injury of myocardium due to ischemia - reperfusion includes cardiac contractile dysfunction, arrhythmias as well as irreversible myocytes damage. These changes are considered to be the consequence of imbalance between the formation of oxidants and the availability of endogenous antioxidants in the heart. Objective : This study was undertaken to investigate the potential role of etanercept in amelioration of myocardial I/R injury induced by ligation of coronary artery in a mice model.Material & method : adult male Albino mice were randomized into four equal groups.1. Group (1) : Sham group : mice underwent the same anesthetic and surgical procedure as the control group except ligation of LAD coronary artery.2. Group (2) : Control group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours.3. Group (3) : Control vehicle group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice received vehicle of etanercept (normal saline) 5 minutes before reperfusion via I.P injection and.4. Group( 4) : Etanercept treated group : mice subjected to regional ischemia for 30 min by ligation of the LAD coronary artery and reperfusion for 2 hours and mice treated with etanercept 5mg/kg i.p 5 minutes before reperfusion XVIIIResults : Compared with the sham group, the levels of TNF - ? & IL - 1?, IL - 6, Caspase 3 and plasma level of cardiac troponin I increased in the control group but decreased level of Bcl - 2 (p<0.01).Histologically, all mice in the control group showed significant (p<0.01) cardiac injury and apoptosis.Etanercept significantly decreased in myocardium level of TNF - ?, IL - 1B, IL - 6,Caspase 3 , and plasma cTnI (P < 0.01), while significantly increased level of Bcl - 2 (P < 0.01). Histological analysis revealed that etanercept markedly reduced (P < 0.01) the severity of cardiac injury in the mice underwent LAD ligation procedure. : ionsConcluThe results of the present study reveal that etanercept may ameliorate myocardial I/R injury in mice via interfering with inflammatory reactions and apoptosis. : RecommendationAfter studying the results of the present study, the following recommendation to further1. Further measuring the P - selctine and E - selectine to show the effect of etanercept on rolling of eutrophils and platelets that cause further occlusion of blood vessels.2. Further measure the adiponectine (that have a cardioprotective effect ).

العلاقة بين اعتلال الجهاز العصبي اللاارادي الناتج عن السكري مع مستوى اللبتين وامراض القلب حسب فحص ايكو القلب وفحص اجهاد القلب == Relation of Diabetic Autonomic Neuropathy With Leptin And Cardiac Disease Assessed By Echo And Treadmill Test

Author name: زينب فلاح حسن
Supervisor name: احسان محمد عبود عجينة | علاء حسين عباس
General topic: Medicine
Specific topic: Physiology
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: الخلفية : الاعتلال العصبي اللاارادي القلبي له تاثير سلبي على الحياة ونوعيتها للاشخاص المصابين بالسكري. الاعتلال العصبي اللاارادي القلبي يعتبر نوع فرعي من الاعتلال العصبي السكري. هذا النوع من الاعتلال هو الاكثر بروزا لتهديده للحياة وبسبب توفر الفحص القلبي | Background : Cardiac autonomic neuropathy (CAN) has a negative effect on survival and quality of life in people with diabetes mellitus (DM) and is regarded as a subtype of the diabetic autonomic neuropathy. This type of neuropathy is the most prominent focusing because of its life threatening and the availability of the cardiovascular tests that can diagnose it. It has been thought that many factors in DM like leptin causing increase the risk of cardiovascular disease. Researches about the relation of leptin with CAN and cardiovascular disease are yet limited.Aim of study : This study aims to determine the prevalence of CAN in patients with type 2 diabetes mellitus, evaluate the association between CAN the presence of left ventricular dysfunction in these patients, test the association between CAN and ischemic heart disease and assess the relationship between the presence and severity of CAN with fasting serum leptin in these patients.Patients, materials and methods : This cross sectional study included 103 (56 males and 47 females) diabetic patients without hypertension or pre - diagnosis of ischemia. They had attended to the diabetic center in Marjan Medical City in Hilla from March 2013 to February 2014. The patients had undergone thorough assessments that included clinical (history and full examination) and biochemical assessments (measurement of fasting plasma sugar, glycated haemoglobin, lipid profile, blood urea, serum creatinine and serum leptin level). Cardiac autonomic reflex tests were also assessed along with echocardiography and treadmill test. Cardiac autonomic reflex tests included the five tests of Ewing protocol. Patients who had two or more abnormal tests were considered as having CAN (75 patients) and those with less than two abnormal tests were considered as patients without CAN (28 patients). Results : The study found that most diabetic patients (84%) were obese (BMI?25 kg/m2) and most of the diabetic patients presented with CAN (72.8%). Fasting serum leptin level was higher in female than in male in both study groups (with and without CAN). Patients with CAN were older when compared to patients without CAN (p?0.01) and had longer duration of DM (p?0.01) with significantly higher fasting serum leptin levels. The leptin level showed a tendency to increase as the score of CAN increased. However, these changes were not significant (P>0.05). The most abnormal sympathetic response was diastolic blood pressure response to hand grip while the most abnormal parasympathetic response was heart rate response to breathing. On contrary, the lowest abnormal response was found in postural blood pressure test. Echocardiography showed that most of the diabetic patients (89.8%) had a left ventricular hypertrophy by measuring the left ventricular mass index. However, there were no significant changes in echocardiographic results between patients with CAN and those without.Conclusions : This study concludes that CAN is a common complication of type 2 diabetes that affected a large percentage of diabetic patients. The duration of the diabetes and the age of patients are important non modifiable risk factors for the development of CAN. Additionally, heart rate variability is considered as an important test for early detection of CAN. On the other hand, CAN has no impact on the occurrence of left ventricular diastolic dysfunction. Leptin level was high in diabetic patients and even higher in those with CAN and its levels related positively with the severity of CAN. Also, there were high leptin levels in diabetic patients with positive treadmill test results, although these changes were not significant (P>0.05).

تاثير تمرينات تعليمية باستخدام اداوات مساعدة في تنمبة اهم القدرات البدنية والحركية وتعلم بعض المهارات الهجومية بكرة السلة للطالبات == Effect of Compound Teaching Supporting By Exercises Tools According To The Most Important Physical And Kinetic Abilities In Learning Some of The Basic Basketball Offensiv For Female Students A Thesis

Author name: زینب عبد السادة علي
Supervisor name: محمد ناجي شاكر | محمد ياسر مهدي
General topic: Sports
Specific topic: Basketball
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: This thesis includes five chapters Performing the basic skills has a great effect on basketball; it needs a high level of physical and kinetic abilities to achieve the optimum and effective performance of these skills. So the study importance is clarified; using aiding easy to made tools can assist the trainers and the players in learning these skills.The study problem lays in that the exercises of learning the basketball basic skills lacks to the aiding teaching tools, so the researcher attempts to use teaching Education & Scientific Research Offensive for Female StudentsA ThesisSubmitted By : Abdul - Sadah AliTo : - Education for Girls/University of KufaPhysical EducationSupervised by : - Ghnaim Asst. Prof. Dr. Moammed Yasir Mahdichapters; the first chapter represents the.and tools that can participate in developing the physical and kinetic abilities and the basic skills in basketball. The research aims to define the effect of the compound teaching exercises by using the aiding tools in learning some of the basketball basic skills. The researcher hypothesizes that the compound teaching exercises by using the aiding tools according to the physical and kinetic abilities have a positive effect on learning some of the basketball basic skills. The study scope is the human field of the first year students in Physical Education dept./ Faculty of education for girls/ University of Kufa.The second chapter carries the theoretical studies that relating subjects such as the compound exercises, the physical abilities, the kinetic abilities in basketball, thebasic skills of basketball and the previous studies. The third chapter deals with the experimental method. The study society is (21) of the first year students, three of them are separated for the research experiment.The group is divided into two groups (9) students for each; the experimental group uses the teaching tools and the control group uses the available tools.The researcher concludes a set of results that could be summarized asfollowing : - 1 - The aiding tools have an effective role in developing the physical and kinetic abilities and the skills for the experimental group.2 - The experimental group proves superior to the control group in the physical and kinetic abilities and the skills in basketball.According to these results, the researcher recommends the following : - 1 - To emphasize using the teaching tools aiding in developing the physical and kinetic abilities and the skills in basketball understudy.2 - The necessity of paying attention to the physical and kinetic abilities during the learning or teaching the players, recognizing the weak physical and kinetic abilitiesof the game and how to develop it.

تاثير بعض اساليب الاستشفاء في اهم المتغيرات البايوكيميائية وانجاز عدو 400م للمتقدمين == The Effect of Some Medical Treatment Methods On The Most Important Biochemical Variables And Achieving 400M Track For Advanced Runners

Author name: افتخار مطر باقر علي الموسوي
Supervisor name: محمد جاسم الخالدي | سلمان عكاب الجنابي
General topic: Sports
Specific topic: Jogging
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: The problem of our sport fields lies in the demands of some of the coaches to increase the requirements of training which increase the burden on the runner accompanied with low medical treatment necessary for exposing the wastes and comprise the lost energy to be prepared for the next training session, as well as the fact that some of the other coaches do not know methods of medical treatment and which is better.This research aims at the following : 1 - revealing the effect of the treatment methods on the biochemical variables and achieving 400m running by the applicants. 2 - Revealing the optimum effect of the treatment methods on the biochemical variables and achieving 400m running by the applicants.The method of the research and its field procedures : The three groups experimental method is used which is suitable for the nature of the research. The research society reached 17 runner from the teams of Al - Najaf governorate for the advanced runners. A sample of 15 runner was chosen And the sample was divided into three groups, five runners for each group.The first group used Massage chair, the second used the Jacuzzi and the third used the laser. The results showed a statistical difference between the effect of the post - tests and the pre - tests on the biochemical variables and achieving 400m track. While in the comparisons of the post - tests, the significant difference was in favor of the first group in all of the variables except for the Glucose variable.The main conclusions were : 1 - The three medical treatments were effective on activating the blood cycle leading to an increase in the glucose ratio and the ratio of O2.2 - The medical treatment methods had an obvious effect on the speed of disposing the lactic acid and decreasing Co2.3 - The effect of the three treatment methods on the studied biochemical variables had an effect on improving the achievement of the runners.The Recommendations : 1 - The coaches have to program the healing sessions in their training methods and pay a great attention to it.2 - Using the healing session researched in the study within their treatments.3 - Conduct a similar study on a sample of Iraq's champions or the Arab ones in the 400m track to obtain data from samples of higher levels.4 - Conduct a similar study on other sports (200m) or (800m, etc

فاعلية تمرينات دورة الاطالة والتقصير في تنمية القدرة العضلية للذراعين والرجلين وعرض الانموذج بتقنيات الحاسوب لدقة اداء بعض انواع التصويب بكرة اليد == Effectiveness Exercises of Stretch And Shortening Cycle, In Development The Muscle Ability of The Arms & The Legs, And Presenting The Sample By Using Computer Techniques To Accuracy of Some Kinds of Targeting From The Handball

Author name: رؤى علي حسين
Supervisor name: عبد الجبار شنين الجنابي
General topic: Sports
Specific topic: Handball
Degree: Doctorate
Language: Arabic
University location: Najaf
First pages:
Abstract: تضمن الفصل الاول التعريف بالبحث واهميته في استخدام تمرينات دورة الاطالة والتقصير وعرض النموذج باستخدام تقنيات الحاسوب في تطوير القدرة العضلية للذراعين والرجلين ودقة التصويب من القفز بكرة اليد، اذ تعد لعبة كرة اليد واحدة من الالعاب الرياضية الجماعية المهم | First Section : Definition Research This approach identifies and its importance by using stretching and shortening exercises and presenting the sample by using the computer in developing the muscle ability of the arms & the legs, and accuracy of some kinds of targeting from a Jumping Position with the handball. The handball game is one of the sport games taught at our physical education colleges. It is considered one of the most popular worldwide sport, which has local and continental championships. The player of handball has many physical specifications including (strength and duration and flexibility and correspondence), and many skills including using one arm with the ball and the other without it, that is (targeting and dripping, passing and deluding and other skills). Therefore, the new training view has become taking care of everything that make it possible to achieve goals through using more specialized mean of training like exercises of the cycle of stretching and constricting which has a deep effect in developing the muscle ability because it has a great importance to the player of handball in using this ability continuously during performing skills of defense and attack representing in the muscle ability of the arms and the legs. The problem of the Research is represented in the abstaining and weakness of the female team of the university especially in performing some kinds of the targeting from a jumping position, both in its high and frontal poses. The reason of being unable to perform these kinds of targeting is failure of the ball's crossing of the defense line or reaching the goalkeeper in a weak impetus and thus makes it easy to catch or push it away. The Objective of the Research can be summarized as follows : - Getting acquainted with effectiveness of cycle of stretching and shortening in developing the muscle ability of the arms and legs of the girls' university team of handball. - Getting acquainted with effectiveness of presenting the sample by using the computer in developing accuracy of performing some kinds of targeting from a jumping position performed by the girls of the handball team of the university of Kufa. The Impose Research : - - There are differences having statistical connotation between the prior and the afterwards experimentation and to the favour of the afterwards experimentation regarding exercises of the cycle of stretching and shortening in developing the muscle ability of the arms and legs of the girls' Kufa university team of handball. Areas of the Research : - The human Area / the girls' Kufa university team of handball. - The duration Area / from 1 December, 2013 till 1 June, 2013. - The spatial Area / the stadium of the College of Education for Girls / University of Kufa.Second Section : The Theoretical Studies & the similar Studies This approach deals with the special theory regarding the research varieties where, the concept of exercises of the cycle of stretching and shortening and the subjects correlated, and the concept of the muscle ability and targeting skill of handball and its kinds of jumping, and the importance of computer techniques and its uses in learning and teaching, taken into account. The similar studies were also taken into account.Third Section : Methodology of the Study and its Field Processes : The empirical method was chosen in accordance with the nature of the problem. The sample was chosen on purpose from the team of handball of the University of Kufa, and it was divided into two groups each of (10) female players. They got equivalence and accordance before starting the training course through a prior experimentation as a means of collecting information; then an empirical method of the cycle of stretching and restriction was presented, and the sample was presented by the computer techniques for 8 weeks, and an afterwards experimentation was done to get the results which were treated statically through using (SPSS), and which enabled the researcher to recognize the differences concerning the research varieties between the two groups. Fourth Approach : presenting the results and analyzing and discussing them This approach includes the total presentation of all the results through diagram columns and tables and analyzing and discussing them scientifically through using the athletic training literature and learning to achieve the research goals and assumptions.Fifth Section : Conclusions and Recommendations The researcher has reached a number of important conclusions, some of thedm are : - The cycle of stretching and shortening has a positive effect in developing the muscle ability of the arms and legs in the empirical sample of the research. - The presenting of the type by using computer techniques has a positive effect in developing accuracy of targeting from a jumping position of the research empirical sample. - The time of the independent variant represented in the training doses was suitable in creating adaptations expressing the range of development in the muscle ability of the arms and legs and accuracy of targeting from a jumping position.Some of the important recommendations - Generalization of the course concerning exercises of shortening and stretching and presenting the type by using the computer techniques on the athletic teams of the handball team of the University of Kufa, and the sport clubs outside the university. - Using the training course of the cycle of stretching and shortening (as a physical part) and the computer techniques in presenting the type (as a feedback nutrition) of the other athletic games inside the university and the sport clubs outside the university. - Designing and shortening a number of experimentations to measure accuracy of targeting from jumping forwards with the ball in hand which will be more objective in measuring this skill in the girls

المحددات الهرمونية لانتقاء اللاعبين في بعض الالعاب الفرقية بعمر (14 - 16) سنة == Hormonal Determinants For Selection of Players In Some Team Games At (14 - 16) Years

Author name: نسرين حسن ناجي
Supervisor name: نزار حسين النفاخ
General topic: Sports
Specific topic: Physical Education
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اشتملت الرسالـة على خمسـة فصول هـــي : الفصل الاول : التـعريف بالبحـث اما هرمون التستوستيرون هرمون ذكري مسؤول عن تكوين العضلات وله دور مهم بالنسبة لاعداد جسم الرياضي حيث اي زيادة في افراز منسوب هذا الهرمون تؤدي الى زيادة في حجم العضلة.اما اهداف البحث ه | 1 - Identifying hormonal determinants of club players altathamin basketball and Kufa club players hand ball of al - Najaf Province age (14 - 16 years).2 - Identify the standard grades and levels and hormonal determinants of physical and skill tests of club players altathamin basketball and Kufa club players hand ball of al - Najaf Province age (14 - 16 years). The research fields are : Human fields : group of players of Kufa clubs hand ball and altathamin clubs bask ball and hand ball School of the Ministry of Youth by (48) players 14 - 16 years old.Time fields : the period from 5/11/2013 - 10/4/2013Place fields - Labs Sadr medical in NajafSpecialist laboratory of Dr. Rahim Mohammed Jabbar. - Imam Ali (AS). stadiums in center of youth - - Indoors Kufa club.Chabter three : Field research Procedure This chapter includes The researcher used the survey method appropriate to the nature of the search. Formed society and the research sample of (48) players are the players club Al - tethamin basketball cluband Kufa club handball and players of the school handball in najaf , sample experience major hit (27) players are the players club al tathamin and The of players Kufa club, either sample experiment reconnaissance amounted to (13) players (3) players from the club Al - tethamin basketball and (10) players from the school handball , either eight players remaining were excluded because of age exceeded 16 years and Less 14 years, has been used sprains coefficient to know the homogeneity of the sample in the variables length , weight, age ,pulse, blood pressure and temperature, and the coefficient has confined between (+1 - - 1) This shows the homogeneity of the sample in these variables and The researcher followed some of the measures which determine the hormones for the study and it was designed questionnaire includes a range of hormones and presented to experts and specialists in this area was given the degree of each hormone according to its relative importance Reached the acceptable degree of 53% of hormons and all the hormones contained in the registration form has of hormones achieved the highest percentages of accepted except class hormone androstenedione achieved the ratio stood at 37% so it was ruled out. In addition to this questionnaire is designed to contain a range of physical and skill tests were presented to the experts and specialists for the purpose of nominating appropriate tests for the study and researcher adopted the agreement ratios experts and specialists and testing, which achieved the highest agreement rate of 60% has been adopted in the study in Saturday On 02/09/2013 exploratory experiment was conducted for physical and skill tests on a sample of (13) as a player and statistical data has been processed and extracted transactions scientific tests and all the tests have been enjoying high scientific transactions. On 14.02.2013 as of ten o'clock am the main experiment was conducted where the main research sample gathered Kufa Sports Club , the medical team conducted a preliminary tests on a sample search (pulse, pressure, temperature) so as to ensure the safety of members of the team then sample Medical taking blood samples by 5 CC, in the hospital's mobile to the Directorate of Health, then headed the research sample (players club al - tethamin basketball) to the Indoors and to conduct physical tests and then technique has lasted tests on about three hours, including medical examinations where it ended All tests at one Oclok pm Then sent blood samples after saved in folders especially to laboratories Health Directorate Najaf (Sadr City Medical) and some laboratories civil to the lack of Ketat own analysis of some hormones such as hormone (adrenaline) and then two weeks after the receipt of the results of the analysis and has put the results in special forms statistical analysis was processed statistically in the bag (SPSS) as well as to make sure the results as manual Part five : Conclusions and recommendationThis chapter included a number of conclusions and recommendations of the most important : Conclusions 1) The researcher concluded that options players coaches were randomized where I found there are differences between the results of the analysis of hormones when the sample and between the world's natural proportions were represented up attributed secretion from the normal limit for the TSH hormone, growth hormone and hormone T4.2) the researcher concluded that options players coaches were randomized where I found there are differences between the results of the analysis of hormones when the sample and the world's natural proportions The decrease was attributed secretion from the normal limit for T3 hormone, cortisol and testosterone. 3) Featured hormone adrenaline rate among members of the sample is equal to the natural rate, and the reason is that the tests under normal conditions (test conditions), the hormone needed to psychological intensity (effect of sport competitions) 4) to achieve different ratios in the level (above average, average, below average) for physical and hormonal tests and skill approaching or exceeding somehow destined in a normal distribution curve.Recommendations : 1) hormonal determinants of interest and promise of the foundation determinants in the selection of players of different ages and all kinds of sports 2) Encourage coaches urged their players conduct periodic checks on the hormones in the blood as a sign to see their cases physical, psychological When choosing sports in all kinds of sports 3) conduct other research to demonstrate the relationship between hormones and various other sports 4) The adoption of the standard grades and levels achieved by the research sample for the purpose of their calendar hormonally and both physically and skills

التنبؤ بمستوى الاستثارة الانفعالية بدلالة بعض المؤشرات الوظيفية ومستوى بعض الهرمونات لدى لاعبي كرة السلة الشباب == Predict The Emotional Arousal Level In Terms of Some Functional Indicators And Hormones Level For The Young Basketball Players

Author name: علياء حميد عبد العباس
Supervisor name: حيدر ناجي حبش الشاوي
General topic: Sports
Specific topic: Physical Education
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: قامت دراستي على خمس فصول وقد اشتمل الفصل الاول على مقدمة تطرقت فيها الى اهمية البحث اذ ان التعرف على مستوى الاستثارة الانفعالية للاعب من الامور المهمة للمدرب كي تساعده في عملية تشخيص الحالة بشكل دقيق دون الاعتماد على استمارات القياس التي غالبا ما تكون بعي | This study includes five chapters. The first chapter deals with the study importance; recognizing the emotional arousal level of the player is one of the important matters for the coach that it could help him to define the player case exactly, without depending on measurement forms that mostly far from the real case, then treating it correctly. Moreover the coach could predict the player emotional arousal level depending on his physiologic and harmonic status.The study problem is represented by that measuring the psychological status of the player is of the important matters that the coach should be acquainted with, the usual method of measurement depends on measurement forms that do not reflect the real status of the player who might be so shy to answer all the questions correctly. The functional indicators reflects the real status of the player, so the researcher attempts to depend on the functional and harmonic indicators to measure the emotional arousal accompanying sport competition and predicate its level by these variables. One of the aims of this study is to predict the player emotional arousal level in terms of some functional indictors and the level of some hormones for the basketball players. The study hypotheses are : - there is a relation between the emotional arousal and some functional indictors and some hormones concentration for the young basketball players.In the second chapter the researcher reviews the theoretical studies in this field exhibiting the related subjects such as predication, emotion and emotional theories, arousal and arousal theories, the functional indictors, hormones and the basic principles for basketball. The study methodology and field procedures are studied in the third chapter. The researcher adopts the descriptive method , the survey method and the correlative studies. The study samples are the young players of the Middle Euphrates and the South clubs who participated in the excellent series season ( 2012 - 2013), they were 20 players of these clubs. The sample is divides into : - exploration sample, rationing sample and the main application sample. This chapter define the main steps of this research such as : rationing the emotional arousal measurement, defining the functional indicators, as well as the hormones, used in the research, describing the functional measurements, the main experience and the statistical methods. The fourth chapter includes demonstrating, analyzing and discussing the results, while the fifth chapter includes the conclusions and recommendations that are set forth by the researcher. The main conclusions could be summarized as following : - * Deducing a finite predicative formula by which the emotional arousal could be predicated in terms of some functional and hormone indicators.* Adopting the research by the basketball coaches the recognize the psychological and physiological effects on the players.

اثر المنهج التعليمي وفقا لاهم المتغيرات الكينماتيكية للنموذج في تعلم بعض المهارات الاساس في التنس للطلاب == The Effect of The Educational Method According To The Most Important Kinematic of The Modal In Learning Some of The Basic Skills In Students Tennis

Author name: نور يوسف مرهون
Supervisor name: قاسم محمد حسن الخاقاني
General topic: Sports
Specific topic: Physical Education
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: لقد احتوت الرسالة على خمسة ابواب : - الباب الاول التعريف بالبحث : - اشتملت على المقدمة واهمية البحث , حيث تطرقت الباحثة الى تطور المستوى والانجاز الرياضي الحاصل نتيجة لجعل الهدف الاساسي لعملية التعليم والتدريب هو الوصول الى اعلى المستويات التي تكون ذات | The thesis included five chapters, the first one is dedicated for discussing the development on the level of performance resulting from making the basic aim of the learning and training process is reaching to the top levels which has a real value by depending on the basis and bio - mechanical rules affecting the moves. As well as revealing the problems that could be solved through the dynamic analysis of the skill. The problem of the research lies in the importance of linking the educational method with its exercises which are chosen according to the bio - mechanical basis and rules and providing a model for the students and feedback on the basic learning according to the model and the Kinematic variables that aids the students in reaching to an elevated athletic level.The aims of the research included : 1 - setting an educational method according to some of the Kinematic variables of the model in learning the Tennis skills.2 - revealing the effect of the educational method, according to the Kinematic variables, on some of the land Tennis skills.3 - revealing the values of some Kinematic variables of the studied skills of the study sample.The hypotheses of the research included : 1 - the educational method exercises affect some of the Kinematic variables values.2 - the Kinematic variables affects learning the skills of Tennis.In the second chapter, the research tackled many subjects of similar theoretical studies which included the bio - mechanic concept and its components and the importance of the Athletic aspect. It also discussed the dynamic analysis concept and its importance to the Athletics.The third chapter included the methodology of the research and the field procedures. The researcher used the experimental method by the two controlled groups method. The sample included 30 student and it was divided into two equivalent groups, the control and experimental groups. The control group adopted the traditional method teaching, while the experimental group followed the educational method by one unit in each week. The fourth chapter included the results, analyzing them and discussing the Kinematic variable. Through those results, the researcher reached the goal of the study and the hypotheses were verified.In the fifth chapter, the conclusions were mentioned. They were : 1 - the educational method exercises positively affected some of the kinematic variables as well as learning the skills of front low throwing.2 - watching the student of the skill model before giving the educational unit will reveal the mistakes in the moves and the reason why they happened.Most of the distance results of the experimental sample revealed an obvious improvement by approaching the values of the model's variables which is an indication of the improved level of both skills

المجتمع المدني في العراق بعد عام 2003 ودوره في بناء الديمقراطية == The Civil Society In Iraq After 2003 And Its Role In Building Democracy

Author name: جميل جعفر البدر
Supervisor name: سعدي كريم سلمان
General topic: Political Science
Specific topic: Political Science
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: It is obvious that the subject of the civil society at the Arabic countries in general, and in Iraq in particular, was not apparent as a social and political practice and reality. There is No doubt that the Iraqi society, just like other countries, passed through the idea of the oneness due to the dominant and comprehensive political leadership upon the economical fields and all the social activities. As soon as the change took place on The 9 April 2003, the civil society organizations have been largely spread, in addition to what is circulated about the active role the civil society is supposed to have in political - decision - making, the political culture that stems from the direct relationship between the civil society and democracy. As long as there is a valid and participating role to the civil society in the democratic process, that will be reflected positively at the democratic experiment the thing that makes it a successful experiment, and vice versa. Consequently, and as a response to the New Iraq reality, the title of the research is, " The Civil Society in Iraq After 2003 and its Role in Building Democracy".The research relied upon more than one approach in completing the research requirements. I adopted the historical approach, was adopted, the descriptive approach, the systemic analysis approach, the legal constitutional approach. The research came into three parts : the first part is dedicated to explain what is the civil society and divided into three chapters. The first chapter talks about the development of civil society in the western thought, so as to introduce a summary about the stages and transitions of the concept of the civil society throughout the philosophical, social, and political thinking. The second chapter came to explain the civil society in the Islamic thought by looking at the origins and principles of Islam and how much they harmonize with the principles and values of the civil society through proceeding research on two standards; the theoretical standard and the practical applied standard. The third chapter explain the conceptual frame of the civil society at the light of the definition, characteristics, contents, and objectives of the civil society. The second part brought up the theme of civil society and democracy.This part holds three chapters : the first one talks about the relationship between the civil society and the state with what it represents of intersection and integration. The second chapter talks about the relationship between the civil society and the political culture through illustrating the tools and mechanisms those form the work basis of the civil society and the role they play to enrich individuals with political culture by mentioning the political culture notion, it's elements, types, and its relation to the civil society, then explaining the functions of the civil society in anchoring the democratic culture. The third chapter covered the relationship between the civil society and the political participation. It explains the notion of the political development, the political participation, and the connection between the civil society and the political participation. Finally, the third part concentrated on the role of the civil society in the democra??c transi??on a??er 2003 in Iraq. The first chapter talks about the relationship between the civil society and the state before and after 2003. The second chapter talks about the rela??onship between the civil society and the political culture in Iraq by showing the structure of the Iraqi people, and showing the role of the prevailing sub - cultures; tribes and religious references - Shia and Sunni - and their connection to the political culture, the prevailing patterns of the political culture in Iraq, and the role of the civil society a??er 2003. The last chapter was all about the relation between the civil society and the political participation by men??oning the reality of the poli??cal par??cipa??on before 2003 in Iraq, the deterioration of the poli??cal par??cipa??on a??er 2003, showing the images of the political participation and the role of the political participation in them.The conclusion holds the researcher's most important conclusions and recommendations those he brought about to finish the research.

العلاقات العراقية الاماراتية بعد عام 2003 الواقع والمستقبل == The Relations Between Iraq And United Arab Emirates After 2003 Reality And Future

Author name: عمار احمد اسماعيل المكوطر
Supervisor name: علي هادي حميدي الشكراوي
General topic: Political Science
Specific topic: Political Science
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: بحثت هذه الرسالة في واقع العقات بين الع ا رق ودولة اما ا رت العربية المتحدة من خل د ا رسة العوامل المؤثرة فيها سواء اكانت عوامل الاجتماعية ام الاقتصادية ام عوامل عسكرية ام سياسية. وتم استش ا رف مستقبل تلك العقات الثنائية من خل تحليل واقعها بين الدولتين ك | This research deals with the subject of the reality of relations between Iraq and United Arab Emirates, through the study of the factors affecting them, whether social, economic, military or political factors.Threw the political relations between the Un

مراكز الابحاث ودورها في عملية صنع القرار عند الازمات في العراق : دراسة ميدانية == Think Tanks And Their Role In Decision Making At Crises In Iraq : Case Study

Author name: عزيز صادق عزيز
Supervisor name: كامل حسون القيم
General topic: Political Science
Specific topic: Political Science
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: The study on field research supports the use of the questionnaire tool in monitoring the relationship between thank tanks and decisionmaking in Iraq during the crises, through a series of questions to the decision makers who had their official positions f

تعديل الدستور واثره على نظام الحكم في الدولة == Amendment of The Constitution And Its Impact on The System of Government In The State

Author name: شامل حافظ شنان الموسوي
Supervisor name: ميثم حنظل الغزي
General topic: Political Science
Specific topic: Political Science
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: The political and constitutional order of each state is a reflection oftheir political, economic, social, cultural and fit a constitutional system ofa particular country, you may not be suitable for another country, butwhat works for a constitutional s

اثار النزاعات المسلحة على معاهدات حقوق الانسان : دراسة في اعمال لجنة القانون الدولي

Author name: محمد عدنان علي زبر
Supervisor name: عباس عبود عباس الخزرجي
General topic: Law
Degree: Master
Language: Arabic
University location: Najaf
First pages:

الرقابة المتبادلة للحكومات المحلية في قانون المحافظات غير المنتظمة في اقليم رقم 21 لسنة 2008 المعدل : دراسة مقارنة == Control Of Mutual Local Governments In The Law Of The Provinces Irregular In The Province Of 21 Of 2008 Average Comparative Study

Author name: نزار محمد جاسم الموسوي
Supervisor name: غازي فيصل مهدي
General topic: Law
Specific topic: Administrative Law
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: After 2003 Iraq has witnessed a shift in its administrative system. After it was decentralized theoretically and centralize practically. The Iraqi legislature has been adopted by a decentralized system politically and administratively. It made the first system as a base to regulate the relationship between the region and federal authority. And made the second one under which the relationship between the central government and the provinces which are not organized in a region.Governorates not organized law has reversed in the territory No. 21 of 2008 amended as possible parliamentary system, which was brought by the Iraqi Constitution properties, to suit the local government level in the governorates not organized in a region by organizing supervisory relationship to local governments, similar to a large cross - censorship boundary between parliament and the government in a parliamentary system.Governorate councils and advisory councils practice a significant control role on the on local executive bodies oversight function varies depending on the level of the Administrative ,Unite ,which these councils represent and it includes all of the executive committees activities except for the court and military contingent and colleges and institutions.The control is not limited on the activities of the general local facilities but includes the main personal of these committees as governors and senior positions. This controlsubstantiate through the authority of governorate on their discharge or exemption of their positions in accordance with the terms stated by the law.However, these councils cannot perform its control role effectively on the local executive bodies except through clear mechanisms for the exercise of this jurisdiction and we see through this research that the mechanisms set by the law is not sufficient to exercise this kind of control effectively.In return for the powers granted by law to local councils to the heads of administrative units in the recent granting of authority in the face of these local councils, but these control enjoyed by the local government some obstacles may sit making exercise supervisory work is not the level of ambition.

التنظيم القانوني لعقد التشغيل المشترك == Legal Regulation Of Joint Operation Contract

Author name: عيسى عبد الجبار اسماعيل
Supervisor name: غازي فيصل مهدي
General topic: Law
Specific topic: Administrative Law
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: We study and consider holding joint operation in General and total starting with the definition and finally end the joint operation contract and statement of jurisdiction and how Iraqi courts jurisdiction to hear disputes arising from this contract relying on analytical approach adopted in induction and deduction and comparison between the views of scholars and texts of laws and judicial rulings and reached to hold interoperability human administrative contracts in nature , Whereby the Department undertakes and called (the concession - granting authority) to a special law called the obligor a management and use of public facilities at his own expense and fee payable to beneficiaries which are profitable and have a fixed - term contract (usually short) advanced stage is similar to the concession contracts with many international administrative and administrative contracts but differs with her on some properties. And holding joint operation is a new kind of contract types, is a groundbreaking product of contemporary life contributes to the development of public facilities, themed mostly infrastructure projects such as ports, airports and railways and road and air transport and sea and can apply this type of contracting in many sectors including the oil industry and others, and that the Administration not only functions in accordance with this contract to ensure the execution of the contract But interfering in guiding implementation and by choosing the most appropriate ways to ensure the smooth running of the General Annex, while adhering to the principle of legality when issuing administrative decisions, in order to derive rights from the contractor as a public authority and which shall ensure the smooth running of the General Annex and native rights these rights are constant for the Administration, even if not included in the contract : 1. for management power to amend certain terms of the contract relating to the General Annex, at its sole discretion, 2. the management authority of the sanction in the case of breach of contractual obligations unilaterally without having to crack.3. the management authority to terminate the contract unilaterally before it has finished The rights enjoyed by the administration corresponding obligations upon themselves and are committed, which is committed to implementing its obligations stipulated in the contract and management rights are, the joint operating contract ends in one of two ways, either a natural end to expiry, and either premature end before expiry, with access to consensual solutions to common operating contracts and dispute them amicably and arbitration according to Iraqi law, the Iraqi civil courts have jurisdiction to consider the joint operating contract disputes and have full jurisdiction in consideration. The study showed the need of developing countries for such type of contract, to provide financing for projects without having to download the State budget additional financial burdens or resort to foreign loans, as well as providing advanced technology and management expertise and technical, the survey regarding the legal nature of the joint operation contract, these contracts are of administrative contracts in nature and subject to common law rules , And cannot be a private law contract, the Administration will prevail over the will of contracted, they sponsor and guarantor of functioning of a public utility, the study showed that this type of hiring needs to advance preparations for hiring or contracting project and the negotiation phase, feasibility phase and from both sides of the contract if the negotiation phase, feasibility phase the Administration set conditions that include determining the duration of the contract And select the required technology and technical specifications as well as the financial aspects of the project, and the personal implementation contractor to contract because of these contracts on the personal account idea in choosing a contractor and as a general rule no contractor may waive the contract because another contractor, contractor run and manage a project to provide services to beneficiaries, as committed to maintenance of equipment, machinery and equipment and replace any faulty ones and the training of national cadres on their use and maintenance , And as committed to equip the project with modern technology, and at the end of the period re annex to management and in good condition and is one of the most important fundamental obligations in the common operating contracts in addition to a Palace. Joint operating contract is a legal system and a sophisticated and flexible contractual terms of procedures of negotiations and a feasibility study and on both sides to avoid potential loss and damage with a note to the contract is no cooperative relationship uplifts to form or generate new moral character (company) and that's what strengthens what about avoiding losses and this contract is one of the most important means of trade and international deal to developing countries and in special circumstances such as Iraq and you want to get the aid in Modern technology of developed countries and access to advanced technology and machinery and equipment in addition to the methods and systems and modern technologies of management and operation and the training of its cadres, and the idea of a joint operation is a partnership between the public and private sector which needs a high degree of trust and cooperation and transparency, integrity and professionalism and expertise among the limbs. The basis for this search is carried out by the Ministry of transport of the joint operating contracts for the years 2009 and 2010 and its proven success where the Ministry convert most self - funded companies from losing companies into profitable companies with sophisticated mechanisms of action resulting from the experience and professionalism of its top management (Minister) and transparent dealings and integrity that enjoyed by these mechanisms and effective solutions for example is holding a joint operation and who returned to the Ministry of financial profits and the entry of foreign investments As well as modern and sophisticated means and management and operation of public facilities and the development and training of its workforce and increase size notes revenue Ministry and companies during the years 2008 and 2009 and 2010 compared to previous years. Joint operating contract features (create, manage and operate or manage, operate or operate shared) that belongs to an area or a particular sector but its flexible recruiting in all sectors and activities and could change depending on the public and industry can benefit from a contract manufacturing or joint manufacturing management contract or contract creation and management and joint manufacturing and the oil and electricity. Etc. with retention of its President, in accordance with the regulations of the relevant sectorial precooked and benefit from the experience of the Ministry of transport in 2009 and 2010 exclusively something needs to hold seminars and conferences for the purpose of idea and promoting it, that second side to hold interoperability is investment and introducing foreign capital and get advanced technology and modern techniques and training Iraqi cadres and learn modern management and operating methods

المسؤولية الجزائية لعضو مجلس النواب : دراسة تطبيقية في ظل دستور العراق لسنة 2005م == The Parliament Member Pe.... Responsibilit

Author name: اسد عبد الله شناوة الحمداني
Supervisor name: علي حمزة عسل الخفاجي
General topic: Law
Specific topic: Constitutional Law
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: Of the rulesg eneral,k nown on the legall evel. : : '' rt the penal law undertakesth e responsibilityo f defining the a' : is that are considereda s crimes and fit punishment for each crime, thi : ' I, called the principle of crime and punishmentl egality

الحدود الفاصلة بين القصد الاحتمالي والخطا غير العمدي : دراسة تحليلية مقارنة == The Difference Between Probable Intent & Non - Intentional Error An Analytical Contrastive Study

Author name: هدى عباس محمد رضا الشماع
Supervisor name: علي حمزة عسل الخفاجي
General topic: Law
Specific topic: Civil Law
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: There are two images for the immaterial element of the crime, either intentionally crime or intentional error, and either intentionally crime or intentional has contiguous borders has made thisjuxtaposition to distinguish between them something difficult,

المسؤولية الجنائية للقنوات الفضائية : دراسة تاصيلية تطبيقية مقارنة == Satellites Channels Penal Responsibility A Comparative Applied Rooting Study

Author name: ليلى حمزة راضي حمادي شبر
Supervisor name: علي حمزة عسل الخفاجي
General topic: Law
Specific topic: Criminal Law
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: Satellites` channels are considered of the most important modern media and the most effective one that exceeded the international limits or the national sovereignty, so the world events are heard and seen ate the moment of their occurrence shorting time a

اثر بناء الجملة في توجيه المعنى في سورة مريم == The Affect of Sentence's Syntax In Guiding The Meaning In Surat Maryam

Author name: علي نور ادريس المحنا
Supervisor name: سيروان عبد الزهرة هاشم عبوة الجنابي
Specific topic: Language
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: Koran text can be regarded one of the most elegant text that descended from the heaven.The best text of the heaven at all since, it's the only text has descended with wording, meaning and without distortion or fabrication.The Koran text is still firm un

بنية الكلام بين المتحاورين في النص القراني : دراسة تفسيرية == Speech Structure Among The Conversers About The Qur’anic Text : Interpretational Study

Author name: وجدان مهدي حسين الخالدي
Supervisor name: صباح عباس عنوز
General topic: Islamic Sciences
Specific topic: Interpretation
Degree: Doctorate
Language: Arabic
University location: Najaf
First pages:
Abstract: The structure is a regarded as a way that connects between the human self and the recipient as being a container of the intents which the speaker seeks. There is a relation between the structure and the attitude of the speaker and listener or reader. The Holy Qur’an has concentrated on this phenomena related to the reader or listener, because the Holy Qur’an is a guiding and preaching book with humanitarian functions for all of the humans in every time and place. Its concentration is on the recipient, therefore, the contextual structure in the Qura;nic text fulfilled its role in achieving those functions. The studier of this text, especially the conversational one, find it very deep, difficult and rich with visions, therefore, I went through this subject depending on Al - Mighty Allah, and convinced and eager to write in this field. This study has endowed me with a lot of knowledge; therefore, it came as follows : 1 - The preface : it included a definition and a historical view of the concept of speech, conversation and structure, it came under the title : Structure, Speech and Conversation - Critical approach”.2 - First chapter : in it, the structure of speech between the conversers is tackled on the structural and semantic levels, and its effect on interpretation. The structure of the word on this level becomes valuable and its expressive ability within the structural context through the text which includes the relations of building the sentence and connecting its rules.3 - The second chapter is entitled “The Structure of the Conversation in the Qur’anic text and its Interpretive Effect on Conversation Patterns”, the Qur’an is accurate in the terms used to express its aims and intents in various contexts in conversations and styles taking into consideration the situation and the occasion. And when reading them carefully, we can sense the nature of the conversation, whether it was external (direct or indirect) or internal.4 - The third chapter is dedicated to reveal the effect of the structure of the conversation on the interpretational methods represented by the method of the Qur’an - Qur’an interpretation, Qur’an - Prophetic tradition interpretation and Qur’an - Rhetoric interpretation.After going through the subject, the following results have been reached : 1 - There is a difference between the speech and the conversation; the speech is greater than the conversation, for the speech is full of different styles which could be unrelated to the conversation, but the conversation is part of the speech. 2 - The structure of the conversation in the Holy Qur’an is not subject to time or place, but in fact it is a comprehensive conversation that is related to every time and place for the recipient and it holds interpretational functions and other functions as well.3 - The structure of the conversation in the Holy Qur’an with all its various types is preceded by a preface which becomes an opening for the mechanisms of conversation, which is something not familiar in the human conversations. Therefore, this matter constituted a miraculous point in the Qur’anic conversation, where the structure of the conversation in the Holy Qur’an became an extension of the structure of the preface which is reflected in the interpretational coherence of the subject.4 - The research discovered a miraculous point that has never been noticed by others, whenever the rhetoric performance in the structure of the conversation in the Holy Qur’an varied, the coherence increased. On another hand, this coherence will serve the interpretation of the text
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الاصول الدينية للتعايش الانساني في الاديان السماوية : دراسة الكتب المقدسة في ضوء القران الكريم

Author name: عبد الرزاق رحيم صلال الموحي
Supervisor name: عبد الامير كاظم زاهد
General topic: Islamic Sciences
Specific topic: Religion Basics
Degree: Doctorate
Language: Arabic
University location: Najaf
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منهج الاستدلال القراني عند اهل البيت (عليهم السلام) : دراسة تفسيرية

Author name: صادق عبد الله راشد الحسن
Supervisor name: صباح عباس عنوز
General topic: Islamic Sciences
Specific topic: Islamic Sciences
Degree: Doctorate
Language: Arabic
University location: Najaf
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ايات عتاب الانبياء (عليهم السلام) في القران الكريم : دراسة تحليلية

Author name: زين العابدين عبد علي طاهر الكعبي
Supervisor name: ستار جبر حمود الاعرجي
General topic: Islamic Sciences
Specific topic: Fiqh
Degree: Master
Language: Arabic
University location: Najaf
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الاعجاز السردي في القصص القراني : دراسة تفسيرية قصة موسى (عليه السلام) انموذجا == The Narrative Inimitability Of The Koranic Stories (Interpretational Study) The Story Of Prophet Moses (P.B.U.H.) As An Example

Author name: حليم عباس عبيد
Supervisor name: صباح عباس عنوز
General topic: Islamic Sciences
Specific topic: Islamic Sciences
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: The importance of the research lies in the light of the narration for its great importance in communicating the Koranic discourse and its effect on the recipient who remains attracted to the narrative style. The style followed in narrating the stories has a great effect in comprehending the facts and accepting the meanings which serves in revealing the function of communicating the ideas of the text especially in preaching and guidance for the recipient.The aim which the researcher pursued is to reveal the effective inimitability of the holy Koran, on one hand, and on the other hand, the researcher aimed to comprehend the term “narration” and the Koranic narration structure. The purpose is to prove that that the Koranic narration structure is a miracle in itself. This is a proof that Koranic miracle is a miracle for all times and places.In addition, narration participates in the interpretational process development of the Koranic text, therefore, the researcher conducted a procedural process on the level of both narration and interpretation and revealing the relation between them. This led the study to be varied between the analytical, objective and descriptive method.The study is divided into a preface and three chapters. The preface included revealing the basic concepts of the title. In it the origin of narration and its roots in the Arab and western thought through referring to its historical path and its rise.The first chapter came under the title “Inimitability and the Nature of the Koran Stories”. It is an attempt to reveal the Koranic Inimitability, on one hand, and the Koran Narrations Inimitability, on the other. The chapter included six sections.The second chapter is entitled “the Structural Inimitability in the Koran Narrations Inimitability” which is an attempt to reveal the Koran Narrations Inimitable narrative structure of the Koran stories, taken the story of Mousa as an example for application, it also included six sections.The third chapter, “the Features of the Narrative Koran Narrations Inimitability in the Koranic stories” discussed some of the stylistic features of the Koranic text taken the story of Mousa as an example for application, it also included six sections.Finally, the research is concluded with a conclusion in which the main results are mentioned followed by a list of references. The main results reached through the study are; Inimitability of the narrations lies in its uniqueness in the Koranic text which has no match ever. The Koranic story is not compatible to any literary critical standards. As for the element of time, each story in the Koran has its own time and the time element in the Koranic story is absolute which has no ties except for the past. As for the characters in the story, they are not divided into fixed patterns, but instead, it focus on the role of each character and its positive and negative effects compatible with the time of the story.
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الاجتهاد الفقهي بين الاصالة والمعاصرة

Author name: حسين كاظم عزيز
Supervisor name: عبد الامير كاظم زاهد
General topic: Islamic Sciences
Specific topic: Islamic Sciences
Degree: Doctorate
Language: Arabic
University location: Najaf
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الحقوق الفكرية : دراسة بين الشريعة والقانون == Intellectual Rights Study Between Al Shari' A And Law

Author name: جواد احمد كاظم البهادلي
Supervisor name: محمد حسين علي الصغير | علي يوسف الشكري
General topic: Islamic Sciences
Specific topic: Islamic Law
Degree: Doctorate
Language: Arabic
University location: Najaf
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الحصانة الدبلوماسية في الشريعة الاسلامية : دراسة مقارنة بالقانون

Author name: مصطفى جعفر عجیل الابراهیمي
Supervisor name: عباس ال كاشف الغطاء
General topic: Islamic Sciences
Specific topic: Islamic Law
Degree: Master
Language: Arabic
University location: Najaf
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فقه الشعائر الحسينية

Author name: صلاح نصر حسن الاعرجي
Supervisor name: رؤوف احمد محمد الشمري
General topic: Islamic Sciences
Specific topic: Islamic Sciences
Degree: Doctorate
Language: Arabic
University location: Najaf
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فقه المعاملات العقارية في العراق : البيع انموذجا == Jurisprudence Of The Real - Estate Transactions In Iraq Selling As An Example

Author name: رؤى محمد صادق محمد جواد الحكيم
Supervisor name: محمد علي هاشم الاسدي
General topic: Islamic Sciences
Specific topic: Islamic Sciences
Degree: Doctorate
Language: Arabic
University location: Najaf
First pages:
Abstract: Through investigating the issue of real estate transactions in Iraq and revealing the rules of selling, we can list the following results that are reached : 1 - Al - Mighty Allah is aware of every detail and determined a rule for each one to be applicable and easy for people.2 - What is meant by the real estate transaction is the land and all what lies upon of building or plants and is subject to the rules of selling and others.3 - The jurists divided the lands according to several considerations but the most comprehensive one is according to its ownership.4 - The public property is for all Muslims and has no deed unlike the individual property.5 - The public property is different from the partnership property and the endowment property. 6 - The public property is a property of the whole nation and the guardian should run it in a way that serves the public benefit.7 - The Imam ownership is the position of the Imamate and not a personal property of the Imam and it allows a personal right for the one who runs it.8 - Through the research it has been revealed that the deed of the land cannot be owned and remains under the control of the Imam. 9 - The matter of running provides priority to take action except for the ownership. 10 - The ways of construction can be taken from the lexicons, history books and narrations. 11 - When suspecting whether the land can be constructed or not, it’s better not to.12 - Most of the lands in Iraq are conquered.13 - it is not allowed to sell the lands of Iraq that are conquered which proved with evidence, it allowed to sell the right utilization and not the deed of the land. 14 - It is not allowed to sell Al - Anfal lands, but the Imam has the right to sell the right to run the land for whom who desires. 15 - It is proved that the land is owned by Allah, his prophet (p.b.u.h.) and the Imam after him, they allowed running the land until the return of the missing Imam.16 - The final result reached is that we can apply the rules of land on the lands of Iraq.
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الخيارات في الفقه الاسلامي : دراسة في الادلة والتطبيقات == Alternatives In Islamic Jurisprudence A Study In Evidence And Applications

Author name: عمار محمد حسين محمد علي
Supervisor name: صاحب محمد حسين راضي نصار
General topic: Islamic Sciences
Specific topic: Islamic Sciences
Degree: Doctorate
Language: Arabic
University location: Najaf
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Abstract: The following are a number of the cognitive results reached through the research : 1 - Since the subject of alternatives is obligatory contracts, the research has tackled the contract in its both types, the general and the specific which is the connection of two wills and their conformation to device a legal obligation.2 - The alternative in its linguistic sense is the choice, preference, rejection and authorization between two matters or more. Terminologically, it is the legally created right by the legislator or one of the contracting parties or both, by which the contractor has the right to repeal the contract or sign it.3 - There are several utterances related to the alternative like repealing the contract either by one of the contractors or both or for legal reasons the contract would be repealed.4 - Some of the alternatives are fixed whether it was chosen or not in some cases rather than the others, the same for their evidence.5 - If there is a flaw that has never been mentioned before, the contractor has the right to repeal the contract. This is proved by evidence from the Quran and the prophetic tradition.6 - The condition of flaw can be true for the seller to and follows the same rules which cannot be valid unless several conditions are met.7 - In case the deceit in the contract was proven, the contractor has the right to demand refund in case it was impossible to return the commodity, otherwise he has the right either to return the commodity or demand refund to decrease the damage.8 - If the product is sold for much more than its value, the buyer has the right to return the product. This is proved by evidence from the Quran and the prophetic tradition.9 - The jurist did not agree on this right, some applied it to all types of transactions and other to the cases when the price is much higher than the value of the product, and this is valid when all the demanded conditions are met.10 - The jurists agreed on the right of testing the product and in case something was wrong with the product, the contractor has the right to repeal the contract, this also supported by several evidence and demands several conditions that should be met.11 - I mentioned all the alternatives devised by the jurists without mentioning the evidence of each one. The first alternative mentioned is the right to repeal the contract in case the both parties are still in the same venue of signing the contract.12 - Some of the jurists has agreed to the previous right supporting their opinion with several evidence and in analogy with the opinion of the prophets companions who supported this right, while others rejected it saying that the contract would be obligatory right after it is signed by the approval of both parties. 13 - This option is valid in all types of transactions and not in other domains as it lacks evidence for that like the contracts which implies the probability of repealing in its nature. This right is conditioned by several terms.14 - The alternative of conditioning is proved by different ways that one of the contractors can set a condition to have the right to repeal the contract within a certain duration and is permitted for both the seller or the buyer or a third person. Several evidence has been mentioned to support this right as well as the need by both parties to consult experts to prevent any harm
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