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دراسة وبائية على طفيلي المشعرات المهبلية Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقولة جنسيا وتاثير بعض المستخلصات النباتــيـــــة على نمو الطفيلي في الزجاج في مدينة كركوك == Epidemiological Study On Trichomonas Vaginalis & Some Associated Bacteria That Causing Sexual Transmitted Diseases & Effect of Some Herbal Extraction On The Parasite In Vitro In Kirkuk City

Author name: محسن عز الدين سليمان
Supervisor name: ابراهيم شعبان داوود | محمد عبد العزيز قادر
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: بلغ العدد الكلي للعينات التي جمعت وفحصت للفترة من بداية شهر تموز 2007 الى نهاية شهر مايس 2008 (2345) عينة لكلا الجنسين مــن مستشفيات كركوك للتحري عن وبائية طفيلي المشــــــعرات المهبليـــــــــة Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقو | A total (2345) specimens were collected & examined from both sexes, from the beginning of July 2007 till the end of May 2008 attending Kirkuk hospitals for epidemiological study of Trichomonas vaginalis & some sexual transmitted microorganisms. The vaginal examinations were done by gynecologist, two high vaginal swabs were taken from (250) women & a cervical swab from some women with vaginal discharge. General urine examination & blood group with Rh factor were performed for each woman with vaginal discharge. Direct microscopic examination, staining & culture of swabs were performed on different methods for diagnosis of Trichomonas vaginalis, followed to diagnose Candida albicans, Neisseria gonorrhoeae, Chlamydia trachomatis & Treponema pallidum as well as some other bacterial groups. The rate of Trichomonas vaginalis was (2.8%) & Neisseria gonorrhoeae (0.8%), while no Chlamydia trachomatis & Treponema pallidum recorded in this study. The highest rate of Trichomonas vaginalis was among the age group between 15 - 29 years (3.6%), the rate of Trichomonas vaginalis infection was higher among illiterate (5.7%) than educated ones. The highest rate of Trichomonas vaginalis was recorded among those with blood group O & Rh (+ve) (3.8%). The same result was found regarding other microorganisms in both sexes. Statistically the results were not significant.The rate of infections in females was higher than males in all infection except Neisseria gonorrhoeae was recorded highest rate in males(35%). The percentage of mixed infections with Trichomonas vaginalis was Candida albicans (1.6%) & B - hemolytic streptococcus (0.4%). The maximum infections rate in vaginal swabs were Staphylococcus aureus (42.3%) & lowest rate of Neisseria gonorrhoeae (2%). Regarding the distribution of microorganisms isolated from urine samples of females suffering from vaginal discharges, the highest rate of infection was Eschirechia coli (22.2%) & lowest rate of Neisseria gonorrhoeae (2%) respectively. The rate of isolated microorganisms from urethral discharge of males were Neisseria gonorrhoeae (35%) & Escherichia coli (5%). Antibiotics Ciprofloxacin & Amikacin showed good inhibition activity against all pathogenic microorganisms. Regarding the effect of different concentration of metronidazole & medical herbs to the Trichomonas vaginalis invitro, metronidazole showed clear effective inhibition at concentration (1.25%) during 24 hours. The inhibitory effect of Zea mays (both alcoholic & watery extractions) was greater than Apium graveolens & Foeniculum vulgare. At concentration (2.5% & 5%) of Zea mays in both extractions was almost identical to metronidazole. Six solvents were used by thin layer chromatography (TLC) in order to separate alcoholic & watery extraction of medical herbs, only three of them A, B & F gave good results in separating herbal extraction with differing in its component. By measuring relative flow rate (Rƒ) of all compounds that separated and determind. The chemical compounds of each herbal extract were established & included alkaloids, flavonieds, amines, pigments, oils, carbohydrates, phenols, purines & tanines.

تقييم استخدام الانترليوكينات في تشخيص تسمم الدم الجرثومي لدى الاطفال حديثي الولادة في تكريت == Evaluation Uses The Interleukines For Diagnosis of Neonatal Sepsis In Tikrit

Author name: هبة حازم صالح
Supervisor name: موسى جاسم محمد الحميش | عاشور رفعت سرحت
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: ان الهدف الرئيس لهذه الدراسة هواستخدام الانترلوكيناتInterleukin - 8, Interleukin - 10 (IL - 8, IL - 10) كعلامة تشخيصية للتسمم الدموي الجرثومي لدى حديثي الولادة. تضمنت الدراسة 45 عينة دم من الاطفال حديثي الولادة الراقدين في ردهة الاطفال في مستشفى تكريت الت | The main aim of this study was uses the Interleukines IL - 8 and IL - 10 as diagnosis marker for neonatal sepsis. This study involved 45 blood samples, were taken from neonates admitted to the pediatrics - neonatal wards at Tikrit Teaching Hospital who were clinically diagnosed as sepsis for the period from September 2011 until March 2012. The ages of neonates were 1 - 30day, and this blood samples were taken for the purpose of determining the levels Interleukines IL - 8 and IL - 10 and identify (WBC and C - reactive protein). Seventeen samples were taken from the blood of neonates without of septicemia as a control group samples. The results of the current study showed non significant differences in total white blood cells count for patients with sepsis when compared with a control group. and showed non significant differences in total white blood cells count for patients with sepsis as gestation birth, sex and birth weight compared to the control group. Regarding level of C - reactive protein there was no significant differences between the control group and the patients with sepsis as gestation birth and sex. and showed a significant differences in C - reactive protein level for patients compared to the control group as birth weight. The Interleukine levels showed that a significant increase in the level of interleukin - 8 (IL - 8) for all patients mals and femals, preterm or full - term and who there weight < 2.5 or >2.5kg compared with a control group. this results showed that the relationship between IL - 8 and WBCs was not significant differences at (p<0.05). and for the level of interleukin - 10 the present study showed a significant increase for patients with sepsis as gestation birth, sex and birth weight compared to the control group, the relationship between IL - 10 and WBCs was not significant differences at(p<0.05). The relationship between IL - 1o, IL - 8 and WBCs was not significant differences. when comparison The results as end result for sepsis show is the absence of significant differences (p<0.05) for patients who became good and who had complications compared with the control group. At last, the study concluded that the Interleukines IL - 8 and IL - 10 levels increase in early time for Inflammation ;So its agood diagnosis marker for neonatal sepsis.

عزل وتشخيص بعض انواع الجراثيم المسببة لالتهابات الزائدة الدودية في مدينة تكريت == Isolation And Identification of Some Baceterial Speices Which Caused Appendices Infections In Tikrit City

Author name: عبد عبد الله محمد الجبوري
Supervisor name: اميرة محمود محمد الراوي | علي صالح حسين الجبوري
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة جمع 75 عينة من الزائدة الدودية (Appendices) مباشرة بعد استئصالها من المرضى الراقدين في مستشفى تكريت التعليمي ومن كلا الجنسين، الذين خضعوا لعملية استئصالها وللمدة من اب عام 2008 ولغاية نيسان 2009. عزلت الجراثيم من العينات باستخدام الاوساط الز

تاثير اضافة عناصر الكالسيوم والمغنيسيوم والحديد الى الوسط الغذائي في حياتية خنفساء الطحين الصدئية Tribolium castaneum (Herbst) Tenebrionidaci) Coleoptera : ) == Effect of Calcium, Magnisium And Iron Elements With Various Nutreint Sources On Biology of Tribolium Castaneum (Herbst) Tenebrionidaci) Coleoptera : )

Author name: حوراء ياسين احمد عبد
Supervisor name: برهان مصطفى محمد الدليمي | عواد شعبان داود الناصري
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تناولت الدراسة اختبار تاثير اضافة ثلاثة عناصرمعدنية الكالسيوم (Ca) المغنيسيوم (Mg) الحديد (Fe) في سطين غذائيين (الطحين والجريش) لصنفين من الحنطة (ابو غريب والعز) في حياتية خنفساء الطحين الصدئية الحمراء Tribolium castaneum (Herbst). اذ استخدمت العناصر بترا | This study includes testing the effect of addition of three elements (Ca, Mg, Fe) in flour and crushed partially debranned wheat for two kinds of wheat : Abu - ghareeb and Al - is in the life of Tribolium castaneum (Herbst).using the elements under different concentrations (0.01 - 0.03 - 0.05) g.to know their effects in Tribolium inculuding : (the number of eggs, period of incubation eggs, number of Larvers, number of pupal, number of external insect, new generation) after adding to nutreint base for the two kinds of wheat.This experiement must be in dark incubation at 35±2°C & relative humidity 75±5% during one generation the result show most important role of nutreint element in life of insect (the number eggs, period incubation eggs, number of Larvers, number of pupal, number of external insect, new generation). on side of period incubation eggs period generation there is not any mentioned effect and the most meaningful one is in kind of Al - Is comperad with Abu - ghareeb. It is noticed the maxi effect for Calisum element.It achieved the highest level in : (the number eggs, period incubation eggs, number of Larvae, number of pupal, number of external insect, new generation) to ratio of flour kind of Al - Is and achieved maxi number : (the number eggs, period incubation eggs, number of Larvers, number of pupal, number of external insect, new generation)in concentration (0.05)g.while the highest in ratio of generation agents in (Fluorine)element ratio(76.2) under concentration (0.03)g, it achieved the less ratio in the dead insect kind of Abu - ghareeb7s the highest in average number eggs, number of larvers for calisum element.while the highest value in average number of pupal, number of external insect, new generation to (Magnasium) element it is achieved the highest in number of eggs, number of Larvers, number of pupal, number of external insect, in concentration (0.05)g and the higher of average ratio new generation is (66) and the higher ratio new generation (79.3) is concentarte (0.03)g.It is noticed the relationship between nutreint element and life of insect is parallel, that is, concentrates increased, number of growing also increased except (Fluorine) element the relation is inversion. on side of crushed partially debranned wheat the effect is more meaningful in kind of Abu - ghareeb than in Al - Iz.Calicum element shows acceptable results, it appeared the higher in : (the number eggs, , number of Larvers, number of pupal, number of external insect) in Abu - ghareeb and new generation in Al - Iz. In the test of the effect of nutreint element agents in life of this insect according to the nutril base for both kinds.the result show that nutrient base of flour is more effective than the nutrient base crushed bartially debranned whaet in all steps of insect life for both kindc in flour maxi In flour maxi number in new generation about (62.5)g and the main ratio of new generation in crushed partially debranned wheat (36.3) for Abu - ghareeb kind (6.7)in flour. (61.1) crushed partially debranned wheat For Al - Is which shown the same features of the results of the effect in nutrient base without adding any elements to its life of insect

تاثير استخدام عقار الكلوميفين ستريت والقهوة العربية وفيتامين E على بعض المعايير الفسلجية والكيموحيوية والنسجية في الارانب البيض المعرضة للاجهاد التاكسدي ببيروكسيد الهيدروجين == Effect of Clomiphene Citrate, Coffea Arabica And Vitamin E Use On Some Biochemical, Physiological And Histological Parameters In Albino Rabbits Exposed To Oxidative Stress Induced By Hydrogen Peroxidase

Author name: اسماء خالد مطني محمد
Supervisor name: زيد محمد مبارك المهداوي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تم في هذه الدراسة اختبار فعالية عقار الكلوميفين ستريت Clomiphene citrate والتحري عن تاثيراته في نسيج الرحم والخصى وكذلك الهرمونات الجنسية والتكاثرية Follicular Stimulating Hormone(FSH), Luteinizing Hormone (LH) , Prolactin, Estrogen, Testosterone, Progest | This study includes the effect of clomiphene citrate drug in uterus and testes tissue and sex, reproduction hormone (Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) Prolactin (PRL), estrogen, progesterone, testosterone) and in Some Biochemical and Physiological Parameters as : WBCs counts, glucose, total cholesterol (TC), triglycerides (TG), High density lipoproteins - cholesterol (HDL - C), Low density lipoprotein - cholesterol (LDL - C), Very low density lipoproteins - cholesterol (VLDL - C), Total protein, albumin, globulin, glutathione (GSH), malondialdehyde (MDA) concentrations in males and females Albino rabbits sera which have oxidative stress induced by hydrogen peroxide (0.5%) for (20) days, compare with the control rabbits.The study involud : - comparison the effects of vitamin E effects and (100mg\kg) body weight of arabica coffea.The animals distributed and randomly divided to(8) groups (each group included 8 rabbits : 4 males+ 4 females) as follow : control group, Hydrogen peroxide (0.05) group, (2mg\kg of B.W) clomiphene citrate (c.c.) group, (2mg\kg of B.W) clomiphene citrate group, (500mg\kg of B.W) vitamin E+(10mg\kg of B.W) clomiphene citrate, (100mg\kg of B.W) Arabica coffea group, Hydrogen peroxide (0.05) +(100mg\kg of B.W) Arabica coffea group and (10mg\kg of B.W)(c.c.) +(100mg\kg of B.W) Arabica coffea group. The results revealed : - 1 - The treatment of males and females of Animal by clomiphene citrate (2 and 4 mg\kg of B.W) cased significant increase at leve (P? 0.05) in concentration of the FSH, LH, estrogen and progesterone hormone, and Glucose, Triglycerides, Globulin, LDL - C, VLDL - C and Glutathione in both male and female compare with the control group, while the same treatment caused increase the concentrations of Testosterone and Prolactin for males, whereas in females the treatment showed increase in Testosterone and Prolactin concentrations Compare with the control. Also the treatment caused decrease Albumin concentration in male and no significant (P? 0.05) changes had been showed in the female s. Respect to total protein, WBCs count there were no significant variation (P?0.05) had been recorded for both sexes compare with the control group. So the result showed Significant increase (P? 0.05) in HDL - C in male only compare with the control group. 2 - The induced oxidative stress caused significant increase at level (P?0.05) WBCs count, Glucose, Total cholesterol, triglycerides, VLDL - C, LDL - C, MDA concentrations for both sexes compare with the control group.While there were no significant variation had been recorded in Albumin and Prolactin concentrations in Females and total protein concentrations for both sexes compare with the control group in another hand there were significant increase (P<0.05) in concentrations of HDL - C, Total protein, Albumin, Globulin, Glutathione, Progesterone, Testosterone, Estrogen, FSH, and LH of male compare with the control group.3 - The treatment of Rabbits (male and female) with (100mg\kg of B.W) Arabica coffea revealed significant decrease (p ?0.05) in concentration of FSH, LH, estrogen, glucose, total cholesterol, triglycerides and VLDL - C for both sexes, and PRL, testosterone , glutathione and albumen in concentrations for male compared with the control group while there treatment. 4 - Treatment males and females albino rabbits by high dose vitamin E caused significant decrease in concentration of FSH, estrogen, total cholesterol, Triglycerides and Globulin for both sexes, compared with the control group, Whereas caused also a significant decrease in concentrations LH, PRL, testosterone in male, and caused a significant decrease in concentration HDL - C in female. Whereas caused also significant increase in concentration of the glucose, MDA, glutathione and the total number of white blood cells for about sexes, and caused also significant increase in concentration of the hormones PLR, testosterone in female only. Whereas caused also a significant decrease in concentrations of HDL - C, LDL - C and VLDL - C in male, whereas no significant variation in concentration of total protein, albumin for both sexes, and in concentration of LDL - C and VLDL - C in female compared with the control group.5 - Treatment males and females albino rabbits by high dose vitamin E, clomiphene citrate, Arabica coffea and H2O2 (0.5%) showed important histological changes in testes and uterus compared with the control group. Treatment males by clomiphene citrate caused significant increase (P<0.05) in sertoli cells, Leydig cells, spermatids and Spermatogonia in male, whereas in uterus tissue treatment caused significant increase in granular cells, hypertrophy smooth muscular and increase cytoplasm size compared with the control group.6 - Treatment by high dose vitamin E and Arabica coffea caused damage in somniferous tubules decrease in sertoli cells, Leydig cells, spermatids and spermatogonia in male, whereas in uterus tissue treatment caused effected uterus gland and loss of normal form and the incident of degeneration, necrosis in the surrounding cells and decrease in vassals blood compared with the control group.The present study showing that to drug clomiphene citrate play an important role to activation the oogenesis and spermatogenesis through stimulating gonadotrophic Hormones(FSH, LH). Also the study showed the role of coffea arabica to induce the infertility through it's impact on the thyroid gland which responsible for the oogenesis and spermatogenesis.the result also showed that the high dose vitamin E working on the accumulation of free radical causing oxidative stress damaging body.

التحري عن فيروس التهاب الكبد نمط B باستخدام تقنيات ELISA وReal Time PCR في دم ولعاب المرضى في محافظة الانبار == Detection of Hepatitis B Virus By Using Elisa And Real Time PCR Techniques In Patients' Blood And Saliva In Al - Anbar Governorate

Author name: ثائر عبد الله حسن الدليمي
Supervisor name: امين سليمان بدوي الجنابي
General topic: Biology
Specific topic: Biotechnologies
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة في مختبرات قسم علوم الحياة في كلية التربية وفي مختبرات مستشفى الرمادي التعليمي العام ومستشفى الرمادي للنسائية والاطفال ومختبرات النظائر المشعة في بغداد للفترة من 1/11/2011 ولغاية 1/5/2012 والتي تضمنت التحري عن فيروس التهاب الكبد نمط B في | The present study has been carried out at the laboratories of the department of biology at the college of Education and the laboratories of the public - teaching Al - Ramadi hospital and Al - Ramadi hospital for women and children and laboratories of Al - Nathaer Al - Mushe'ah in Baghdad for the period from 1/11/2011 to 1/5/2012. The study consisted of the investigation about Hepatitis B virus in the saliva as compared with serum by using the ELISA and Real Time PCR techniques as well as the detection about antigens and antibody to this virus in serum. It also seeks about the liver functions enzymes and evaluating the extent of its importance for diagnosing liver disease resulted that virus. However, the obtained results showed that HBsAg antigen was found in 99.4% and (1.101± 0.335) mean ±SD in serum as compared with 48.6% in saliva with a (0.330±0.278) mean ±SD, that gave a noticeable increase when comparing it with the control group which was (0.0039± 0.0024) and (0.0017±0.0014 ) for serum and saliva respectively. The value of T test for serum and control group was (16.25) , while the value of T test for saliva and control group was (4.18). The proportion of correlation between saliva and serum in ELISA test was equal to (0.361) with high significance of test while the value of T test for them was (28.902) with high significance of test. The results have also stated that the age groups involved in the experiment contributed as being high proportion of infection in the serum, but in the saliva, these proportions were different and it has been found that the high proportion of infection was 53.3% in the group (10 - 20) yrs., While the lowest proportion was 38.4% in the (31 - 40) yrs. Moreover, it has been concluded that the number of males exceeded the number of females, but the number of females was the most infected with HBsAg in the experiment group. However, the total number of males was 91, It was found that 90 males have positive test to the HBsAg in serum with a proportion equals 98.9% whiles 42 males have a positive test to the HBsAg in saliva with a proportion equals 46.1%. on contrary, the number of female was 84 female and all of them had a positive test to the HBsAg in serum with a proportion 100% and 43 females have a positive test to the HBsAg in the saliva with 51.1 % as compared with control group which included 15 males and 10 female. All of them were negative to the HBsAg test in serum and saliva... The levels of HBV DNA were determined in the samples of positive saliva to ELISA test by using Real Time PCR. 95% from tested serum samples were positive to the viral load While in saliva 50% from the samples was HBV DNA positive. The results also showed that soluble antigen HBeAg has been detected in 24 patients with 13.7% from a total 175 patient as compared with the control group that showed a negative result to this antigen. As for the antibodies, it has been found that anti - HBc IgM was found in only 5 patients with 2.5% while anti - HBc IgG was found in 170 patients with 97.2% from the total number of patients. The control group revealed negative result for these two antibodies. The anti - HBeAg was in 150 patients with 85.7% from the total number of patients. There were no positive patients to this antibody in control group. The anti - HBs wasn’t found in any patient but it was found in the half of control group. This emphasizes to immunize against HBV in those members. The results also exposed that there was a noticeable increase in liver enzymes that it was found that ALT the elevated levels of were found in 76% of patients. As compared with the control group which has normal values 100%. As for AST enzyme was elevated in 60.6%, As compared with the control group which was normal 100%.It has been observed that ALP enzyme was elevated with a 38.4% in the experiment patients as compared with the control group which was normal 100%. Finally, the total values of TSB was elevated in 35.5%, As compared with control group which was normal 100% with (0.52±0.19). Nevertheless, it has been noticed that it cannot be judged about the person's blood on the basis that it is healthy in case of absence of surface antigen unless it is certain that his/her blood is free from HBV DNA by using the technique of Real Time PCR - since many patients who heal from past infection or their infection in a time of passive and amounts of HBV DNA can't be diagnosed by using the ELISA technique

تشخيص بعض النواتج الحيوية لانواع من السيانوبكتريا المعزولة من مياه وترب مدينة تكريت ودراسة فعاليتها على بعض انواع البكتريا المرضية والحيوانات المختبرية == Identification of Some Bioproducts From Some Species of Cyanobacteria Isolated From Water And Soil From Tikrit City And Study Their Biological Effects On Some Pathogenic Bacteria And Laboratory Animals

Author name: ايمن عوني سليم جاسم
Supervisor name: حميد سلمان خميس المهداوي | احسان محمود عبد الله
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة الحالية عزل وتشخيص (19) نوع من السيانوبكتريا من عينات مياه وتربة طينية ومن الصخور من (5) خمسة مواقع في مدينة تكريت ضمن محافظة صلاح الدين، وقد تم اختيار ثلاثة انواع من هذه السيانوبكتريا وهي : Anabaena oryzae, Nostoc linckia, Anabaena laxa اعتم | The current study included Isolation and Identification of (19) species of cyanobacteria from water, clay soil and rocks samples, collected from (5) sites in Tikrit, salah Al - den province. Three species were chosen each species represent the environment which collected from it. These species were Anabaena oryzae, Nostoc linckia, Anabaena laxa. The effects of some environment factors on growth and biological products quantity has been studied in the three species above. The effects of the temperature showed that 26°C was the best for growth and biological products quantity than 16°C and 36°C. While the pH effects showed that pH 7.6 was the best for growth and biological products quantity than pH 6.6, 8.6. Three light intensities were used 1250, 2500, 5000 lux were used. The results showed that the light intensity of 2500 lux was the best for daily growth and quantity of extracted biological products. While the study of the effects of addition of macronutrient and micronutrient to Asm - 1 media which used for culturing of the three species of cyanobacteria by replacement of NaCl by NaNO3 in the medium, showed that there is an increase in daily growth and quantity of extracts of biological extracts, also the addition of FeSO4.7H2O and NaNO3 together showed further increase of daily growth and biological extracts quantity. The biological products has been identificated by HPLC - MS, IR and NMR. The results showed the presence of the neurotoxin Anatoxin - a and the hepatic toxin Microcystin in its various forms MCYST - LAba, MCYST - YM(O), [D - Asp3, Dha7]MCYST - LR and MCYST - YA in the biological products of Anabaena oryzae. The results also showed the presence of the hepatic toxin MCYST - YM(O) and [D - Asp3, Dha7]MCYST - LR in the extracts of the two spices of cyanobacteria Nostoc linckia and Anabaena laxa.The results also showed that the Muller Hinton Agar media is better than the Blood Agar media in testing the activity of these extracts in inhibition of the pathogenic bacteria, the method of holes is better than saturated discs, and the solvent DMSO (Di - Methyl Sulpho Oxide) is the better in testing of biological activities, while the filtrate of cyanobacteria did not shown any activity for inhibition of the pathogenic bacteria which include : Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus Vulgaris, Staphylococcus aureus. The results showed the high capability of inhibition in concentrations of 1000, 2000, 3000 µg/ml of the extracts of the cyanobacteria Anabaena oryzae, Nostoc linckia and Anabaena laxa on the above pathogenic bacteria and some times more efficient than antibiotics used for comparison, and the concentration 3000 µg/ml was the most effective.The effects of biological extracts of the three species in concentrations of (1000, 2000, 3000) µg/kg of body weight on tested albino mice, using injection and feeding methods, was studied. The results showed poisoning nervous symptom for the first few hours, while the results showed hepatic poisoning symptom on the dissecting animals. The anatomic symptoms included changes the colour of the liver to darkening and appearance of necrosis, in addition to becoming lobbed and swollen some times with bleeding.While the poisoning effects on dissecting tissues of livers of the animals showed a simple swollen and some times assured in all of the liver accompanying with increase in growth and cell division, as well as increases in lymphatic cells inside the tissue and in general in liver tissue. The lymphocyte seemed bigger than for normal cell with multi - nuclei in white blood and increase in cell size and destruction of liver tissue. Nostoc linckia was the greater urging for cell division consequently probably more induce of cancer in the liver of the animals, while the biological product of Anabaena laxa showed more poisonous for liver cell than the biological products of Anabaena oryzae, the least effective in cytotoxic and urging cancer cell in liver of the animals.

اثر بعض المستخلصات النباتية في سمية مبيد الديازينون ضد يرقات وكاملات خنفساء الطحين الصدئية الحمراء Tribolium castaneum (Herbst) == The Effect of Some of Extracts of The Plants With Disinfectant Against The Larva And Pillars of Red Flour Beetles Tribolium Castaneum (Herbst)

Author name: توركان احمد حمد حسن
Supervisor name: برهان مصطفى محمد الدليمي | عواد شعبان داود الناصري
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اشتملت الدراسة الحالية على دراسة التاثير السمي للمستخلص الايثانولي والمائي لثلاثة نباتات وهي : الزعتر Thymus vugarisوالشيح Artemisia herba alba والنعناع البستاني mentha piperita اذ استخدمت مستخلصات الاوراق لكل نبات واختبرت سبعة تراكيز لكل مستخلص (10000,

تاثيـر المستخلص الكحولي لاوراق نبات ذيـل العقرب Heliotropium europium وفيتامينC في بعض الجوانب الفسلجية والكيموحيوية لذكور الجرذان المعرضة للكرب التاكسدي == Effect of Heliotropium Europium Leaves Alcoholic Extract And Vitamin C In Some Physiological And Biochemical Parameters of Male Rats Exposed To Oxidative Stress

Author name: صفا احمد عبد القادر محمد دنيدل
Supervisor name: صاحب جمعة عبد الرحمن | عويد هيدان الخزرجي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: هدفت الدراسة الحالية الى معرفة تاثير المستخلص الكحولي لنبات ذيل العقرب Heliotropium europium في وزن الجسم وعدد من المتغيرات الفسلجية والكيموحيوية كتركيز الهيموكلوبينHaemoglobin (Hb) وحجم كريات الدم المرصوصةPacked cell volume (PCV) والعدد الكلي لخلايا الدم | The aim of the present study was to investigate some effects of the alcoholic extract of Heliotropium europium in body weight and some of physiological and biochemical parameter such as concentrations of Hemoglobin (Hb), Packed Cell Volume (PCV) and the total count of leukocytes (WBCs) and concentrations glutathione (GSH), Malondialdehyde (MDA), Peroxynitrite radical (ONOO - ) , Superoxide dismutase enzyme (SOD), glucose, Total Cholesterol (TC), Triglycerides (TG), High density lipoprotein (HDL - C), Low density lipoprotein (LDL - C), Very Low density lipoprotein (VLDL - C), Atherogenic indices levels, Total protein, Albumin, Globulin, Urea and Uric acid in serum and histological changes in liver of male rats that exposed to oxidative stress induced by hydrogen peroxide H2O2(0.5%) with drinking water for (30) days, and comparison these effects with the effects of the vitamin C as well known antioxidants(250 mg/kg of body weight), for the purpose of this study preliminary experiments were carried out to find the most effective does of plant extracts and it was found that this does was (300 mg/ kg) body weight.The study included(35) male albino rats, (4 - 6) months ages and(245 - 280)gm weight were divided to (7) groups both group with (5) rats as follows : Control group, hydrogen peroxide (H2O2) group, Heliotropium extract group, H2O2+ Heliotropium extract group, Heliotropium extract + vitamin C group, H2O2+ Vitamin C group, H2O2+extract of Heliotropium+ vitamin C group.The results showed : 1. The animal under oxidative stress by hydrogen peroxide caused a significant decrease (p? 0.05) in body weights and values of Hb , PCV, GSH, activity of SOD enzyme, HDL - C, total protein, albumin, globulin and compared with the control group. While it caused a significant increase in WBCs, MDA, ONOO. - , glucose, cholesterol, TG, LDL - C, VLDL - C, Atherogenic indices levels(I, II, III) and urea compared with the control group.2. The treatment of animals exposed to oxidative stress induced by hydrogen peroxide with extract of Heliotropium europium showed significant increase in the weights of animals, Hb, PCV, GSH, activity of SOD enzyme, HDL - C, total protein, albumin and globulin compared with a hydrogen peroxide group. While this treatment showed significant decrease in WBCs, MDA, ONOO. - , glucose, cholesterol, TG, LDL - C, VLDL - C, Atherogenic indices levels (I, II, III), and urea when compared with a hydrogen peroxide group.3. Compared with a hydrogen peroxide treatment of rats exposed to oxidative stress by hydrogen peroxide with vitamin C, extract of plant and vitamin C with hydrogen peroxide group showed the results were similar to the results of treatment plant extract with hydrogen peroxide group.4. The treatment plant alcoholic extract and vitamin C showed a significant increase in the level GSH, MDA, activity of SOD enzyme, HDL - C, globulin compared with the control group. And showed a significant decrease in animal weights, WBCs, ONOO. - , LDL - C, VLDL - C and urea compared with a hydrogen peroxide group. While not shown significant differences in the values of Hb, PCV, glucose, cholesterol, TG, Atherogenic indices levels total protein and uric acid compared with the control group.5. The treatment alcoholic plant extract group showed increase in the weights of animals and values Hb, PCV, WBCs, GSH, MDA, Triglyceride, VLDL - C, Atherogenic indices levels, globulin and urea compared with the control group. Showed significant decrease in the HDL - C and albumin compared to the control group. while did not show significant difference in ONOO. - , activity SOD enzyme, glucose, cholesterol, total protein and uric acid compared the control group.6. The most important histological changes that have occurred in this study have showed treatment hydrogen peroxide compared with a control to swelling hepatocytes with necrosis each and the small size of the nuclei with swelling sinusoid, and the treatment of animals exposed to oxidative stress plant extract has showed a lack of clarity within the cells in hepatocytes necrosis and swelling sinusoid compared with a hydrogen peroxide while the treatment of animals exposed to oxidative stress with vitamin C showed sinusoid constriction and showed treatment alcoholic plant extract and vitamin C for the animals exposed to oxidative stress loss nucleus cells with the difficulty of distinguishing sinusoid.

تاثير بعض العوامل البيئية والمايكروبايولوجية في تنقية مياه الشرب لمحطة المجمع السكني في قضاء الدور - محافظة صلاح الدين == Effect of Some Ecological And Microbiological Factors On Drinking Water Purification Plants In Al - Mugama Al - Sakany In Al - Dour City - Salahaldin

Author name: فخر الدين نور الدين رشيد الخفاف
Supervisor name: حميد سلمان خميس المهداوي
General topic: Biology
Specific topic: Aquatic Environment
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة جمع عينات شهرية من مياه نهر دجلة ومحطة مياه الشرب العائدة للمجمع السكني في الدور اخذت العينات من خمس محطات مختارة ابتداء من شهر تشرين الثاني 2007 ولغاية شهر تشرين الاول 2008 لغرض دراسة بعض العوامل الكيمياوية والفيزياوية والبايولوجية السائدة | This study was included collecting of monthly water samples from Tigris river and water purification plant in AL - Mugama AL - Sakany in AL - Dour, These samples were collected from five chosen locations, started from November 2007 until October 2008, To Study The dominance of some chemical, physical and Biological factors Through the year its effects on water purification plants in AL - Mugama AL - Sakany in AL - Dour. These factors included air and water temperature, Turbidity, Electrical conductivity, pH, dissolved Oxygen, BOD, Total alkalinity, Total hardness, Calcium and Magnesium hardness , Chloride reactive nitrite, nitrate phosphate and salinity as well as some Biological factors such as Total number of bacteria and diatoms during the study period. The results of this study shown that there was a seasonal variation in some chemical and physical factors. where the value of air temperature ranged between (3.5 - 40) C and water temperature ranged between (3.4 - 32.5) C.Turbidity value ranged from (2.2 - 22.94) NTU while electrical conductivity ranged between (320 - 590) µs / cm. The results also showed that the water in Tigris river was alkaline, where the pH value ranged between (7.08 - 7.98). and aeration. The avarices value of dissolved oxygen ranged between (3.5 - 10.2) mg/L and The BOD value never increase to critical value, its ranged between (1.58 - 2.37) mg /L salinity ranged between (0.110 - 0.305) mg/L while chloride concentration ranged between (21.78 - 23.82) mg / L. It has been found that total hardness ranged between (170 - 370) mg/L while total alkalinity for the five station ranged between (124.1 - 137.8) mg No2/ L while Micronutrients ranged from (0.388 - 0.449) mg / L and the value of nitrate ranged between (ND - 0. 598) mg No2/ L and (0. 65 - 122) mg Po4 / L. In General where we compare the monthly average for physical and chemical variation for Tigris water with standard world limits applied for grouping of surface water, we can consider the quality of Tigris water is good and suitable for uses as a source for drinking water by using traditional purification of water.While the results of study of Biological factors showed that the total number of bacteria for Tigris water was modularity clear and unsafe for drinking with out treatment include sterilization. High number of bacteria was found in station (1) during February 2008 when it reached (284) cell / ml and the lowest average recorded in station (5) at December 2007 when its reached (50) cell/ml. This study also shown that high number of diatoms were recorded in station (1) when its reached (46860) cell/L at December 2007 and the lowest number recorded in station (5) when it reached (236) cell / L at November 2007. It has been identifies (11) genus and sp. of diatom in this stations and there were :

تحديد مقاومة بعض الجراثيم المعزولة من اخماج في مستشفى تكريت التعليمي للمضادات الحيوية وعلاقتها بالبلازميدات == Determination The Antibiotic Resistance of The Some Isolated Bacteria From Tikrit Teaching Hospital Infections And Their Relationship With Plasmids

Author name: شيماء عبد محمد علي
Supervisor name: وعد محمود رؤوف | خالد عمر محمد علي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: جمعت (140) عينة من المرضى الراقدين لثلاثة ايام فاكثر في مستشفى تكريت التعليمي للمدة من 1 - 8 - 2009 ولغاية 1 - 12 - 2009 بهدف تحديد العزلات الجرثومية الاكثر شيوعا ضمن اخماج مستشفى تكريت التعليمي ومقاومة هذه العزلات للمضادات الحيوية ودراسة العلاقة بين مقاو | One hundred fourti samples were collected from hospitalized patients for three days or more in the Tikrit Teaching Hospital during the period from 1/8/2009 until 1/12/2009 to determine the most commonly isolated bacterial from nosocomial infections in Tikrit Teaching Hospital and their resistance to antibiotics and to study the relationship between antibiotics resistance and plasmid content in the most commonly isolated bacteria and to evaluate the prevalence of these plasmids in the hospital environment. These samples include : (75) Urine, (28) stool, (28) of wound swabs and (9) sputum samples.The incidence of nosocomial infection was (45.7%) of them, urinary tract infections were (50.7%) from (75) samples, followed by wound infections (42.9%) from (28) samples, gastrointestinal infections (39.3%) from (28) samples then pneumonia (33.3%) from (9) samples the nosocomial infections were more common in females and age group 51 - 89 years. Different bacterial species were isolated in this study and the results showed that the most commonly isolated type was Citrobacter koseri in a proporation (21%) of urinary tract infections, followed by the following bacterial species : Escherichia coli (15.7%), Staphylococcus schleiferi (10.5%) , Staphylococcus epidermidis (7, 8%), Proteus vulgaris, Staphylococcus saprophytica, Klebsiella ozaenae, Klebsiella ornithindytica in aproportion (5.2%) for each, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis in aproportion (2.6%) for each.Citro. koseri were also the most common in wound infections (25%), followed by the following bacterial species : E.coli (16.6%), P.mirabilis (16.6%), Staph.schleiferi, P.vulgaris, Prov.stuartii and Prov.rettgeri in aproportion (8.3%) for each. Citro.koseri were also the most common in the gastrointestinal tract infections in a proporation (36.3%), followed by E.coli (18.1%), S.flexneri (18.1%), P.mirabilisc, Prov.stuartii, Prov.alcalifaciens in a proporation (9%) for each. . While Strept.pneumoniae was the most common(66.7%) in penemonia cases followed by K.pneumoniae (33.3%).The isolated bacteria were tested for antibiotics susceptibility and it has been found that most of these isolates showed high resistance to the studied antibiotics other than Prov. Stuartti was sensitive to all antibiotics except of Rifampicin and Tetracycline. As well as bacterial species belonging to the genus Staphylococcus were sensitive to Amikacin, Staph.aureus was also sensitive to Nitrofurantoin while Staph.warneri was also sensitive to Ciprofloxacin and Nitrofurantoin also Enterobacter aerogenes was resistant to all antibiotics except for Ciprofloxacin and Nitrofuratoin. Eleven of most commonly isolated bacteria which were multidrug resistant were chosen for the curing experiments using SDS to study the relationship of their plasmids with resistant to antibiotics. The results showed that the cured cells of E.coli lost their resistance to Tetracycline Ciprofloxacin and Cefotaxime which Citro.koseri lost their resistance to Tetracycline while K. ornithinolytica lost resistance to both antibiotics Tetracycline and Ciprofloxacin either P.mirabilis lost resistance only to tetracyclin as well as Strept.pneumoniae lost their resistance to Cefotaxime, tracycline and Ampicillin. Which may mean that the genes responsible for resistance to these antibiotics are located on plasmids as proven by the results of electrophoresis.Electrophoresis results also showed that all studied bacterial isolates had the same plasmid profile which may regarded as an epidemiological pointer that plasmids are conjugative which have the ability to transfer antibiotic resistance to other bacterial strains of the same species or to other different bacterial species.

دراسة بعض الصفات الفيزيائية والكيميائية والحياتية لمياه نهر دجلة ضمن مدينة تكريت == Study of Some Physical, Chemical And Biological Properties On Tigris River In Tikrit City

Author name: بشار طارق اسماعيل هلال الشنداح
Supervisor name: رياض عباس عبد الجبار
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تهدف الدراسة الحالية للتعرف على بعض الجوانب الفيزيائية والكيميائية والحياتية لمياه نهر دجلة ضمن مدينة تكريت ولمياه المخلفات السكنية في محطة المعالجة.جمعت العينات لمدة سبعة اشهر اعتبارا من شهر تشرين الثاني 2006 وحتى نهاية شهر ايار 2007 وبمعدل مرتين شهريا | The present study aimed to find out some physical, chemical and biological aspects of Tigris river passed in Tikrit - city, and population residence in the treatment unit.Samples were collected over the period of seven months during November 2006 to the end of May 2007 biweekly. Four stations were selected for sample collections over the distance of 25 km. Two stations were in Tigris river and two were in the treatment unit. The first selected station was close to the old water purification unit in Tikrit - city. The second station was selected at the treatment unit in AL - Dubaee before the mechanical treatment unit. The third station was selected at the treatment unit after the biological treatment. The fourth station was selected in Tigris river about ten km south of the treatment unit at AL - Dijla town. The study involved an investigation of some physical, chemical and biological aspects of Tigris river and the residence waste discharges to the river. This included air and water temperature, conductivity, PH, dissolved oxygen, BOD, Turbidity, total hardness, calcium and magnesium hardness, chloride, total alkalinity, nutrients (i.e., phosphate, nitrite, silica) , and the concentrations of heavy metals (i.e., cadmium and cupper). The biological factor included the total bacteria counts cultured on nutrient agar. Results of the study clarify the close relation between air and water temperature which varied (8.5 - 36) C0 and (9 - 27) C0 respectively. Conductivity values were relatively high and ranged (128 - 3530) microsemens/cm. River water almost was alkaline during the period of the study. PH values of the first and fourth stations were close in the river, although a value of 6.12 was recovered at station on during May. In all stations, PH values ranged (8.8 - 6.12). River water has a good ventilation, dissolved oxygen values ranges (8 - 11) mg/L at the first and the fourth stations, while these values were (0.1 - 1.34) mg/L at the second and third stations. Mean dissolved oxygen values pointed out the availability of good oxygen concentration for the continuity of living organisms which ranged (0.3 - 5.8) mg/L in Tigris river, while BOD values at the stations second and third were high and ranged (5.6 - 131.2) mg/L. Turbidity was high during April at the stations one and four and ranged (2.03 - 788) NTU in all stations. Total hardness in Tigris river was slightly low - very hardness and ranged (120 - 360) mg/L at the first and fourth stations, while these values were (180 - 480) mg/L at stations two and three.The high total hardness values coincided with the high conductivity values. Concentrations of calcium ions responsible on the calcium hardness ranged (211.4 - 120.7) mg/ L was higher than magnesium ions responsible for magnesium hardness range (5 - 265) mg/L.Records of chloride ions ranged (14.16 - 169.99) mg/L at all stations. However, chloride values at stations first and fourth were among the allowed world values and ranged (14.16 - 42.48) mg/L.Alkalinity values were due to the carbonate and bicarbonate and ranged (35 - 725) mg/L.phosphate values ranged (0.168 - 25.2) µg phosphor atom - phosphate/L. Low nutrient values recorded in Tigris river, while the highest values recorded at second station (treatment unit).Nitrite values fluctuated at all stations and ranged (0.078 - 14.778) µg Nitrogen atom - Nitrite /L. Silica values ranged (648 - 5961.6) µg silicon atom - silica /L. Heavy metals values (Cadmium and Cupper) clarify that, Cadmium were (un sensible), except one value (0.008) mg/L that recorded at November, and un sensible for Cupper.Results of the statistical analyses of all involved factors showed significant differences at stations first and second, and between the first and third stations as well as the fourth station. While there was no significant differences between the second and fourth stations. And no significant differences between stations first and fourth.Total bacterial counts indicated high bacterial counts, and the average of total bacterial count ranged (2.605X105, 5.44X108, 2.265X108, 2.385X105) / ml at the four stations respectively, so the Tigris river considered pollutant - very pollutant.

دراسة وبائية وسريرية لمرض الجيارديا Giardiasis في اطفال مدينة الشرقاط وضواحيها == Parasitic Study of Giardiasise Among Children In Sharqat City

Author name: علي حمد طلال موسى الجبوري
Supervisor name: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة لبيان تاثير طفيلي الجيارديا المعوي على صحة الاطفال في قضاء الشرقاط لـ(250) طفل، تم اختيارهم بصورة عشوائية، اذ تراوحت اعمارهم من شهر - 15 سنة وللمدة ما بين شهر تشرين الاول 2006 لغاية شهر تشرين الاول 2007، تم فحص عينات الغائط من كل طفل بال

دراسة بيئية ونوعية لمشروع تصفية ماء الكرخ في الطارمية == An Ecological And Qualitativ Study For Al - Karkh Drinking Water Plane At Al - Tarmia

Author name: احمد توفيق احمد المشهداني
Supervisor name: رياض عباس عبد الجبار
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: نظرا لما لمياه الشرب من اهمية كبيرة في حياة الانسان وضرورة ان تكون هذه المياه ضمن مواصفات ومعايير خاصة, فضلا عن ظهور العديد من مشاكل تلوث مياه الشرب في مختلف مدن العراق. دعت الحاجة لاجراء هذه الدراسة لبيان مدى صلاحية مياه الشرب المنتجة من مشروع تصفية ماء | Owing to the drinking water of great importance in human life and it is important to be under the specifications and standards, as well as the emergence of many of the problems of contamination of drinking water in various cities of Iraq. Needed to conduct this study to demonstrate the viability of drinking water produced from the project filter water Karkh (which is the largest project the liquidation of water in the Middle East, it is feeds many of the cities of Baghdad, on the side of Karkh) and compare the specifications of Iraq and the global standard for drinking water in order to investigate the potential weaknesses in the project and study the possibility of the development of such large projects as a first step to improving the situation of drinking water in Iraq. So were the tests, physical, chemical and biological common to drinking water in the project and divided into four main stations, namely, (station socket raw water, sedimentation basins secondary sand filters, pumps, distribution of drinking water), including nine stations secondary starting of the month of October - 2010 to July - 2011.The results showed high values of turbidity, and electrical condactivety and pH, and dissolved oxygen, and biological dimond of oxygen, and alkalinity, and total hardness, calcium hardness, magnesium hardness, chloride, phosphate, nitrite, silica, fluoride, sodium, potassium, and heavy metals, namely, (iron, copper and cadmium, nickel, lead, chromium and zinc), and the total numbers of bacteria, and total coliform bacteria, the fecal coliform bacteria and E. Coli in drinking water for most of the stations in the Alsta spring and low concentrations in summer and autumn.Values for water temperatures between (4 - 37.5) c, as recorded differences significant between the months of winter and spring, summer and autumn, and the values of brownish discharge ranged between (250 - 0.3) NTU as it showed the differences of the moral high values of turbidity in the winter and fall in the summer and fall in each stage of the treatmant with it, and the values of electrical conductivity was between (520 - 895) Micro Siemens / cm 3. The water Alnehrualcherb in the project was registered as a weak base pH values between (9.7 to 1.7), and it was well ventilated, as the oxygen values were high in the winter months registered clear monthly variation ranging between (5.14 - 2.3) mg / L, either values biologicaldimond of oxygen were values between (4.4 - ND) mg / L and found that basal total stations ranged from every stage of the liquidation potential in increasing concentrations of dissolved oxygen, between (160 - 100) mg / L, and found that drinking water hardness too, as that the values of total hardness ranged between ( - 215 335) mg / L as suited their values directly proportional to both the calcium and magnesium hardness ranging between (197 - 139) and (31.472 - 16.128) mg / L, respectively, while for the values of the chloride ranged between (69 - 25) mg / l, while the fluoride has recorded values ranged between (15 - 0.05) mg / L, with values ranging between sulfate (169.2 - 90) mg / L, free chlorine residual values ranged between (4.18 - 1.1) mg / L, and materials for suspended solids ranged values between (84 - ND) mg / L proven contribution stages of liquidation, all in the rates of removal of different, either TDS fluctuated values between (545 - 315) mg / L, with log Aluminum values ranged between (0.98 - 0.01) mg / L, but for the potassium values ranged between (3.2 - 2.15) mg / L, and sodium values ranging between (6.31 to 3.21) mg / liter.As for the concentrations of heavy metals did not detect any significant concentration of heavy metals measured except for iron ranging between concentrations (9.2 to 8.1) mg / L, so after the industrial plants that contain the droppings of these metals. The nutrient values were recorded between nitrite (0.024 - 0.001) mg / Trosgelt nitrate values between (1.7 - 0.16) mg / l, recorded between ammonia (0.54 - ND) mg / L, placed at either phosphate values ranged between (0.26 - ND) mg phosphorus atom - phosphate / L, while for the values of silica fluctuated between values (5.12 to 5.1) mg atom of silicon - silica / liter. As for the study of signs bacteriological ranged the total numbers of bacteria in air through (24) hours between (555 - 0) × 10 cells / 1 ml, and ranged in numbers for a period of (48) hours between (1186 - 0) 10 × cells / 1 ml, while for the numbers of total coliform bacteria fluctuated between (1700 - 0) cells / 100 liters, and fecal coliform bacteria, they ranged (1100 - 0) cells / 100 ml, and the E.coli bacteria, the numbers fluctuated between (1100 - 0) cells / 100 ml. For to match the drinking waterproduced from the projects liquefaction research found matching the values and concentrations of brownish, and connectivity electrical and pH, and dissolved oxygen, and the requirement is vital for oxygen, and basic college, and chloride, and ten college, and magnesium, nitrite, phosphate, and the total numbers of bacteria, and the numbers of total coliform bacteria, and fecal coliform bacteria and E Coli, and silica with the standard specifications for drinking water the Iraqi and global at all stations.While the study results show non - conformity of calcium concentrations in this study with the standard specifications for drinking water of Iraq and the world.

تقييم فعالية مستخلصات مستنبت القمح Triticum aestivum في بعض الانواع البكتيرية المسببة لاخماج مختلفة == Evaluation The Activity of Germinated Wheat Extractions Triticum Aestivum On Some Bacterial Species That Cause Different Infections

Author name: اسماء عيسى محمود
Supervisor name: رشيد حميد حسن
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: The study had included isolation and diagnosis of three hundred samples for both sexes their ages were between (1 - 60) years of the inpatients and outpatients in Tikrit Teaching Hospital and General Samarraa Hospital during the period from 1/9 / 2011 to 1/3/2012. The infections were distributed among the burns infections, the wounds, urinary tract infections (UTI) and female genital tract infections (High vagina). This study clarified that (192) sample from the total samples showed bacterial growth in percentage 64%. The results of this study indicated to the proportion 58% with (UTI) by (87) bacterial isolation were produced from (150) isolation. The percentage of burns infections were 81.81% from (55) sample which distributed among the first, second and third class burns. The burns of second and third class gave bacterial growth in a proportion 100% while the first class burns gave 64.28%. Concerning the wounds infections, bacterial growth was 62.9% from (62) sample. The infections proportion of (H.V) was 63.63% from (33) sample. The results of the current study showed that the infections in females were higher that in males with respect to (UTI), while there was not considered difference between the males and females about the burns and wounds infections. Differently, the (H.V) infections were just for the females in the ages between (18 - 45). It was clear according to the results of diagnosis that Escherichia coli had the highest proportion of isolations 25.52% from the total isolates followed by Staphylococcus aureus 18.23% Pseudomonas aeruginosa 15.63%, both Klebsiella pneumoniae and Proteus miribilis 10.42% Staphylococcus epidermidis 4.69% Citrobacter freundii 4.17%, Staphylococcus saprophyticus 3.64% both Serratia marcescens and Enterococcus faecalis 2.6%, Streptococcus pyogenes 1.56% and Staphylococcus intermedius had the least percentage 0.5% by one from (192) isolate. The sensitivity of these isolates was tested by (10) antibiotic which included quinolones, aminoglycosides, beta - lactams and tetracyclines antibiotics. Among these antibiotics imipenem and ciprofloxacin were more effective, while the other antibiotics were different either active or not active at all.The wheat germinating was used in this study with the ages (36 - 48) hour and its activity was tested by using some bacterial kinds. After completing several quantitative and qualitative tests on this germinating, the carbohydrates proportion was between 67 - 70%, the protein proportion by folin method was between 10 - 8%, the lipids proportion was 1.5%.Some mineral elements in germinated wheat were estimated which include Calcium, Iron, Magnesium, Potassium, Copper, Manganese, Zinc, Selenium, and Sodium element, active materials were also estimated in the wheat germinating extracts which had inhibitory effect on the microbiology. It was clear by the tests that the germinating contains a lot of Phenolic compounds, Terpeniods, glycosides and steroids. These materials were extracted by using some extracts included the cold water extracts and hot water extracts, alcoholic extracts (ethanol and methanol) and phenolic extract. All the extracts prepared in concentrations (25, 50, 100, 200) mg/ ml. This study showed that the phenolic extract had high effect on the bacterial kinds in comparison with the methanolic and ethanolic extracts at (200) mg/ ml concentration while the cold water extract had less effect on these bacterial isolates in comparison with the above extracts, finally the hot water extract had no inhibitory effect against any isolated bacterial type with all the concentrations. The inhibitory action of these extracts was compared with some antibiotics as a control sample. These antibiotics represents different groups of antibiotics and which contained Imipenem, Ciprofloxacin, Gentamicin, Ceftazidime in addition to Augmentin.In this study the minimum inhibitory concentration (MIC) of the fenolic, alcoholic and watery extracts was determined and the concentration was different according to the extract type, the bacterial type and the isolation site.

التحري عن مؤشرات وراثية وفسلجية لتحمل الجفاف لصنفين من الحنطة Triticum aestivum L. == Investigation of Genetic And Physiological Markers For Drought Tolerance of Two Cultivars of Wheat (Triticum Aestivum L)

Author name: اشواق ابراهيم بشير العبيدي
Supervisor name: شاكر مهدي صالح | عقيل حسين العاصي
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تم في هذه الدراسة البحث عن امكانية ايجاد مؤشرات وراثية ترتبط بمؤشرات فسلجية وذلك من خلال الدراسة على مستويين : ا - المستوى الفسلجي : زراعة صنفين من الحنطة الناعمةL Triticum aestivum في حقول كلية الزراعة/ جامعة تكريت لموسم النمو 2010 - 2011، وذلك بزراعة بذو | This study was conducted to investigate a genetic marker linked to physiological marker through a design an outdoor experiment which includes two parts : - 1 - Physiological part : Included planting two cultivars of soft wheat (Triticum aestivum L.) in the fields of the college of Agriculture, university of Tikrit, during growth season (2010 - 2011). The stage of work had been done by cultivated the seeds of two cultivars of wheat (Sham - 6, Abu - Ghraib) in pots, when they reached the stage of (4 - 5) leaves it had been exposed to two dryness periods 7 and 10 days. It spread with growth regulator Indole Acetic Acid (IAA) (150 ppm) to know which type is more resistance for dryness through estimation of proline concentration as a physiological marker and to indicate the effect of IAA to reduce the dryness effect in some features of growth physiological function and yield component according to complete Randomize Design (CRD). Some markers of drought tolerance have been estimated including : proline content, chlorophyll concentration and the degree of membrane stability then plants were left for harvesting period. The results of the outdoor experiment were : 1 - Exposure the plants to dehydration periods during their growth caused reducing of the most studied characters (leaf area, plant height, tillers number, concentration of chlorophyll A and B and the total chlorophyll), while there was an increase in the content of the proline and the injury index of plasma membrane. 2 - Some characteristic did not showed effect by drought periods (dry weight of plant, number of days of flowering, spikes number, spike length, number of grains/ spike and weight of 1000 grains).3 - Sham - 6 cultivar was superior than Abu Ghraib - 3 in three characteristic (number of days of flowering, leaf area, weight of 1000 grains), while Abu Ghraib - 3 cultivar was superior in the grains number /spike than sham - 6 cultivar.4 - The concentration of IAA (150 ppm) had a different effect. It had a positive effect in the decrease drought for some characteristic (proline content, injury index of plasma membrane, leaf area, plant height, dry weight, spike number and grains yield).2 - Genetic part : DNA has been isolated from fresh leaves of two types of wheat, arrange yields of DNA were in the range of (40 - 150) micro gram per 1.5 gram of plant leaves, and the purity ranged between (1.2 - 1.7). The reaction PCR was done by using 11 primer of the Random Amplified Polymorphism DNA (RAPD) in addition to two pair of specific primer to detect the genes which are responsible for coding of two enzymes which involved in anabolism and catabolism of proline. The results obtained are as follows : - 1 - Five primer of RAPD did not appear any binding site (OPA - 18, OPL - 20, OPA - 11, OPQ - 17 and OPH - 14).2 - Six primer of RAPD have appeared main and monomorphic bands (OPO - 16, OPH - 07, OPN - 16, OPM - 20, OPJ - 13 and OPQ - 06), in addition to the absent of the other bands from some treatments. 3 - The two pair of specific primer have given three monomorphic bands with different molecules weight.4 - The primer (OPH - 07) has appeared a unique band with molecular weight (1670) pb for Sham - 6 cultivar during second drought period (10 days without hormone). These results obtained from this study were identical with physiological part in the ability of both types to drought tolerance

دراسة تاثير مستخلصات نباتي القرنفل والخروع على نمو بعض الفطريات المنتجة لانزيم البروتييز والمسببة لالتهاب الاذن الخارجية == Study Effect of Syzigium Aromaticum And Ricinus Communis Extracts On Growth of Some Fungi That Produce Protease And Cause Otitis Externa

Author name: بتول عمران ذيب
Supervisor name: خليل ابراهيم بندر النعيمي | ذكرى احمد حمادة
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: ضمنت الدراسة عزل وتشخيص الفطريات من الاذن الخارجية في مستشفى تكريت التعليمي - وحدة الاذن والانف والحنجرة والعيادات الخاصة في مدينة تكريت, خلال الاشهر تموز, اب وايلول 2008. جمعت 100 مسحة اذنية من المرضى المشخصة اصابتهم بالتهاب الاذن الخارجية الفطري من قبل | The study was included isolation and identification of fungi from the external ear in Tikrit teaching hospital, the unit of Ear, Nose, and throat (E.N.T.) and Privete clinics in the city of Tikrit during the months July, august, and September2008. 100 ear swabs were collected from the patiants whose diagnosed by Otologists with Otomycosis, and 50 ear swaps were collected from healthy persons in the same city and period time, These samples were examined by dierect microscopic examination treated with KOH 10% with hot flame to ensure the fungal infections, and Culturing on Sabouraud, s Dextrose Agar was done for isolation and identification of types of fungi which responseple for infection, The result of direct microscopic examination and culture showed that 76(76%) were positive for fungi, 64 were positive by direct microscopic examination and culture, and 12 posative in culture only. The most common fungi isolated from patient were Aspergillus Spp.36). (%22 followed by Candida Spp. (%67. 10), Trichophyton Spp(%6.5) , and Penicillium spp. (%3.94%), Aspergills niger appeared as the predominant fungi (%84. 36) followed by Aspergillus flavus (%36. 22), Candida albicans (42. 18%), Aspergills terrous (7.89%) , Candida kruzi, Penicillium spp (3.94%), Trichophyton mentagrophyte, Trichophyton verrucosum (63. 2%) and Trichophyton scheonlieni (%1.31), and no fungi were isolate from the healthy person. Higher percentage of fungi infection were recorded in female 46(60.52%) than male 30(39.47%). and the higher percentage for fungal infection was recorded in the age group (31 - 40) with percentage (23.68%). fungal ear infection was recorded with highest percentage in right ear 47 (61.84%), and for the left ear 29 (38.15%). and high rate of infection was recorded in august (73.69%), Higher percentage of infection was recorded in patient house wives (52.36%), the itching was the most commen symptom , The most habits used to clean the ear by dearty body (51.31%), Ahigher percentage (76.4%) recorded in non educated persons. The results of active ingredients test for Syzigium aromaticum and Ricinus communis revealed that clove dry flower buds contain Flavonoides, Glycosides, Tannins, Volatile oils, Terpenes, Resins, and Phenols, while castor leaves contain Flavonoids, Tannins, Volatile oils, Terpenes, Resins, Saponins and Phenols, pH of clove bud 4.3 and pH of castor leaves 5.8. Aqueous and alcoholic extracts were Prepeared, which belongs to Syzigium aromaticum and Ricinus communis under the study, commercial oil for clove and seed castor were used in the study, the effects of inhibition of Aqueous and Alcoholic extracts and commercial oil had been studied on the growth and protease production on the three fungi which were showed the high ratio appeared among the isolation sample under the study with 5 concentration 10, 20, 30, 40, 50 (mg /ml), and the affected rate of these extracts and oils under the study on fungi were varied. The relation between Aqueous extract of clove bud and fungi growing was increasing relation, While Alcoholic Extract and commercial oil showed preference in the inhibition of the isolated fungi grown while its effect on Candida albicans was increase with increase the concentration, The effect of aqueous and Alcoholic Extract of castor leaves on Moulds showed reverse effect. on the other hand the commercial oil effect was slight effect, and both extracts did not showed effect on the Candida albicans as well as the Commercial oil.When adding Clove Extracts and Commercial oil and in all concentrations dose not gave any production of the Protease enzyme in the solid media, whereas the extracts of castor leaves and commercial oil were showed effects on the production of Protease enzyme, and Aspergills niger was more effected and these extracts did not showed an effect on the Candida albicans.Aqueous and Alcoholic extract and commercial oil to the Clove revealed higher influence rate than the effect of the Castor leaves extract , and in a different rate

تشخيص المسببات البكتيرية والفايروسية لخمج الاغشية السحائية في مدينة الموصل == Detection of Bacterial And Viral Causes of Meningitis In Mosul

Author name: قاسم مصطفى خالد
Supervisor name: علي صالح حسين الجبوري | اميرة محمود محمد الراوي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة اجراء عدد من الاختبارات العيانية والكيميائية على عينات سائل النخاع الشوكي (CSF) cerebrospinal fluid البالغة 152 عينة والتي جمعت من الاطفال الراقدين في مستشفى الخنساء التعليمي ومستشفى ابن الاثير التعليمي في مدينة الموصل لحديثي الولادة لغاية 1 | This study includes performing of macroscopical and chemical and Microbial tests on 152 samples of cerebrospinal fluid (CSF) collected from hospitalized children in Al - khansaa and Ibn - Al - Atheer teaching hospitals in Mosul city with ages ranged from newbornn to 12 years old. Suspected to be infected with meningitis during the interval from August 2009 till April 2010.The results showed that 46.1% of samples were normal with respect to colour and appearance as well as variations in the levels of the three variables including glucose, protein and total cell count with differential cells in the studied samples.The study also included isolation and identification of gram positive and negative bacteria, morphological, cultural and biochemical tests and API were done depending on identification of the isolated bacteria.Many types of isolated gram positive bacteria belonged to the two genera staphylococcus and streptococcus; the results revealed that S.aureus was the more predominant within gram positive causes. Well as many types of gram negative bacteria were isolated represented with Klebsiella pneumoniae, Haemophilus influenzae, Acinetobacter spp. E.coli and Neisseria meningitidis with different percentages with dominance of K.pneumonia and H.influenzae.The study also included screening of some viral causes of meningitis from (35) CSF samples which gave negative culture results by using qualitative Enzyme immunoassays technique using Minividas apparatus.The results showed positive results with 14.2% for two types of virus including Herpes simplex virus (HSV) and Cytomegalo virus (CMV).The study indicated that males were more compared with Females with 55.9% and 44.1% to males and females respectively and highest infection rate occurred in 1 day - 2 years old.Statistical analysis showed the presence of significant correlation between sex, age and the three varieties glucose, protein and total cell count the results showed there is no significant differences between the studied factors.The study also includes sensitivity for some antibiotics, the results showed that gram positive bacteria were the more sensitive to Vancomycin and Cefatriaxone as well as Clindamycin while gram negative bacteria showed sensitivity and resistance percentages of bacteria in general were varied to other antibiotics under study.

دراسة تاثير الملوثات المنبعثة من المركبات على الاشخاص في شوارع مدينة تكريت == Study The Effect of Pollutants Emitted From Vehicales Upon Blood of Workers In Tikrit City Streets

Author name: رغد مقداد محمود الحمداني
Supervisor name: جهاد ذياب محل الجنابي | صالح محمد رحيم العبيدي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: استهدفت الدراسة الحالية التي جرت في شوارع مدينة تكريت قياس تراكيز بعض الغازات المهمة التي تطرحها المركبات الى البيئة وهي احادي اوكسيد الكاربون, ثنائي اوكسيد الكاربون, ثنائي اوكسيد الكبريت (CO, CO2, SO2) وكذلك دراسة نوعية مياه الامطار المتساقطة على منطقة ا | The current study which takes place in the main streets of the city of Tikrit, aims at measuring the concentrations of some essential gases which are emitted by the vehicles of the environment. These gases include Monoxide Carbon, Dioxide Carbon and Sulfur Dioxide (CO, CO2, SO2). This study also aims at investigating the quality of rainwaters which are falling on the area of the study, and conducting some biological tests for them. Moreover, this study aims to identify the effect of vehicles productions upon blood physiology and the construction of some persons who are dealing with them. The samples have been collected during September 2010 till May 2011, and then classified in terms of age and duration of exposure. Alsuqoor Village represents the control group. A sample has been collected from healthy, non - smoker males. The concentrations of some polluted gases emitted from exhausted vehicles are identified. They are significantly high in comparison with the control group and during the whole chapters of the study. The highest level of pollution value is recorded by the gases (CO, CO2, SO2) and at (p< 0.05) level in Albasha Street during the Autumn, whereas the lowest value is recorded in Alzuhoor Street, during the Winter ( ). In addition, some of rainwater characteristics have been measured such as, (Ph, electrical m and the amount of plankton in water). The obtained results show that there is a significant decrease in PH concentration and a significant increase in EC concentration. Moreover, results indicate that the highest concentration of the clot material is in Alarbaeen Street ( ) whereas, the lowest concentration is in Alzuhoor Street ( ). The level of some rare elements has been measured in the blood serum of those who are exposed to (lead, cadmium) and in different periods of times. A significant increase is recorded in the levels of those elements, in comparison with the control group (ps 0.05). As for effect of blood components of those who are exposed to pollutants, the concentrations of both (Hb, Pcv) have been decreased with an increase in the concentrations of (the number of red and white cells and ESR) in comparison with the control group at level (P<0.05). It is also noticed that there is a significant increase in the number of the neutral and acidic white blood cells with a significant decrease in the number of the moral white blood cells at the lymph cell, in comparison with the control group. Results also show that there is a significant increase (P<0.05) in the level of each (Cholesterol, Triglycerides, and Lipoproteins with low density LDL) with decreased concentrations of (High Density Lipoproteins HDL) whereas, there are no significant difference in the concentrations of (Low density Lipoproteins Very high density VLDL). Concerning the concentrations of (Urea, Uric acid, Kreatenin) there is a significant increase (P<0.05) in comparison with the control group and for all the groups of the study. The pollutants have an effect upon the average number of enzyme in the blood serum. There is a significant increase in the average of the enzymes that carry amino (ALT, AST) an enzyme phosphate in comparison with the control group. The study has also shown a significant increase in the Monoaldehyde level MDA and decrease in the glutathione level, under the effect of various pollutants. It is noticed that the blood components as well as the biochemical variable are greatly affected as the period of exposure extended and these biochemical variables become very great. Therefore, most of the differences are existed in the third group whose ages range between (40 and 49) with 9 years of work, followed by the second group whose ages range between (30 and 39) with 7 years of work. Then comes the first group whose ages range between (20 and 29) with 5 years of work, in comparison with the control group

دراسة وبائية لبعض مسببات الاسهال من الاوالي الطفيلية في المرضى المراجعين لمستشفى صلاح الدين العام في تكريت مع دراسة تجريبية لتاثير المستخلص الكحولي لنبات Lantana camara عليها في الفئران المختبرية == Epidemiological Study For Some Diarrhea Causes From Parasitism Protozoa For The Patients That Attended General Salahalddin Hospital In Tikrit With Experimental Study For The Effect of Alcoholic Extract From Lantana Camara Plant On It In Experimental Mice

Author name: رواء محمود هاشم
Supervisor name: توفيق ابراهيم محمد الالوسي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة للمدة من 2010 /11/1 الى 2011/11/1.وتضمنت فحص 1002 عينة من غائط مصابين بالاسهال تراوحت اعمارهم ما بعد الولادة الى اثنتي عشرة سنة من العمر راجعوا مستشفى صلاح الدين العام في تكريت. اشتملت الدراسة على جزءين تمثل الجزء الاول بدراسة وبائية، ت | This study made on the period between (1/11/2010) to (1/11/2011) that included testing (1002) diarrhea patients excrement samples, their ages were between after birth to twelve years that had attended General Salahalddin Hospital.The study included two parts. First epidemiology study, collecting samples and making the laboratory tests that accomplish with 2 methods, the direct method and deposition method to detect the percentage of infected by some parasitism protozoa that cause the diarrhea according to a lot of standards that included, age, sex, class, nature of suckle, number of family members, the source of water, mother education, sequence of the child in his family and the season of infection, the laboratory test assured that from the total (582) , (58.08 %) are infected with parasitism diarrhea compare with other diarrhea cases that was (420) , (41.91%) and reached to the following : - The infection with E.histolytica diarrhea was (492), (%84.5) and infected with G.lamblia diarrhea was (90) , (15.46%). Number of infected males with E.histolytica and G.lamblia parasitism as follows(8.24%)(46.39%) and infected females with E.histolytica and G.lamblia parasitism as follows (7.21%) (46.39%).The highest infection with E.histolytica and G.lamblia parasitism was the ages between (4 - 6)years and the percentage was as follows (4.81%, 29.89%). The highest infection with amebic tissue and G.lamblia parasitism that came from country were (9.10%, 60.82%) also noticed that the raise of infection with E.histolytica and G.lamblia parasitism among children that their mothers had low education less than intermediate (illiteracy and intermediate) as follows (29.8%, 36.5%) for the children were infected with G.lamblia parasitism. And for children that infected with E.histolytica parasitism among children that their mothers had low education less than intermediate (illiteracy and intermediate) as follows (4.29%, 36.5%). The percentage for infants for the first 2 years had been infected with E.histolytica and G.lamblia parasitism for the first 2 years that they depend on industrial suckle as follows (7.73 %), (41.2%). The percentage for infants above 2 years had been infected with E.histolytica and G.lamblia parasitism for the first 2 years that they depend on industrial suckle as follows (9.10%) , (48.96%) also noticed the raise of infection with E.histolytica and G.lamblia parasitism among the children that was the river is the water source, the percentage were as follows (8.59 %), (51.54%). And the infection with E.histolytica and G.lamblia parasitism among the children that was the pipes system is water source the percentage were as follows (6.87%), (23.98%). the infection were very high with E.histolytica and G.lamblia parasitism among the children that lives within crowded families, the percentage was (12.54%), (55.67%). and the infection with E.histolytica and G.lamblia parasitism according to the seasons was dissimilar, and the highest recorded infection with E.histolytica parasitism in winter as follows (35%) and the lowest infection was (11.3%) in summer. Meanwhile the highest infection with G.lamblia parasitism (8.41%) in summer and (1.20%) in autumn.The second part studied the effect of alcoholic extraction from lantana camara plant on growth of E.histolytica and G.lamblia parasitism on mice. The effect was obvious for the alcoholic extraction for the mentioned plant for all extraction on the E.histolytica and G.lamblia parasitism in general, but the most effectiveness extraction to destroy the E.histolytica and G.lamblia parasitism was 1000 mlm/kgm from the alcoholic extraction for the plant lantana camara

التاثير الوقائي لعصير الليمون والمستخلص المائي للمعدنوس في منع التحصي التجريبي المستحدث باستعمال الاثيلين كلايكول وتقليل السمية الكلوية المستحدثة بالمضادين الحيويين جينتامايسين وفلوكساسين في الجرذان == The Protective Effect of Lemone Juice And Parsley Aqueous Extract In Prevent Experimental Urolithisis Induced By Ethylene Glycol And Decrease Renaltoxity Induced By Gentamicine And Floxacin Antibiotics In Rats

Author name: صبا خير الدين ابراهيم الطائي
Supervisor name: زيد محمد مبارك المهداوي | فدوى خالد توفيق
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: هدفت الدراسة الحالية الى معرفة بعض التاثيرات الفسلجية والكيموحيوية والتاثيرات النسيجية للمستخلص المائي لنبات المعدنوس Petroselinum Sativum وعصير الليمون Citrus Linnaeu في كبح تكون حصى الكلى Renal calculi المستحدث بمادة الاثيلين كلايكول Ethylene glycol وال | The study aimed to searching the Physiological, Biochemical and Histological effects of the aqueous extract parsely of petroselinum sativum and lemon juice of citrus linnaeu in inhibition renal calculi formation which induced by Ethylene glycol and decreasing the nephrotoxity casead by the use of Gentamicin and Ciprofloxacin antibiotics. The study included number of Physiological and Biochemical parameters : total white blood cells count (WBCS), Hemoglobin (Hb), packed cell volume (PCV), Total cholesterol (TC), Triglycerides (TG), High density lipoprotein (HDL - C), Low density lipoprotein (LDL - C), Phospholipids, Atherogenic indices levels, Total protein, Albumin, Globulin, Blood Urea, Serum Creatinine, Glutathione (GSH), Maloddialdehyd (MDA), Aspartate amino transferase (AST) and Alanine amino transferase (ALT) concentration, beside the measurement of calcium ions, potassium ions, and magnisum ions concentrations in male and female rat serum which had been induced Oxidatiotion stress by use (0.05) hydrogen peroxide daily for (30)days.Also anather group of rats mouthy feeded with(100 mg/kg of body whight)Ciprofloxacin and the last group of rats feeded with (80 mg/kg of body wight)Gentamicin by muscular injection daily for (14)days. the active dose of petroselinum sativum (parsley) aqueous extract and Citrus linnaeu(lemon juice)had been demonstrated as (250 mg/kg of body whight)and(1.5ml/kg of body whight) successively.the rats distributed randomly (male and female)to (13) groups included (five rats for every group) : control group, Ethylene glycol (Eg)group, (Eg)+parsely aqueous extract group, (Eg)+lemon juice group, Hydrgen peroxide(H202)group, (H202)+ parsely aqueous extract group, (H202)+ lemon juice group, Ciprofloxacin+ parsely aqueous extract group, Ciprofloxacin+ lemon juice group, Gentamicin+ parsely aqueous extract group, Gentamicin+ lemon juice group.The results showed1 - the Treatment rats with Ethylene glycol caused high significant increase (P? 0.01)in concentrations of, TC, TG, Phospholipids, 2nd and 3rd of atherogenic indices, blood urea, ALP, serum creatinine, VLDL - C, AST, WBCs and MDA, while significant (P? 0.01) decrease had been happen in the concentration of albumin, globulin, total protein, HDL - C, GSH, ALT, Hb, Ca, Ma.2 - the Treatment rats with Ethylen glycol and lemon juice and parsley produced high significant increase(P? 0.01) in concentrations of albumin, globulin, total protein, HDL - C, GSH, ALT, Hb, Ca, Ma. while significant (P? 0.01) in concentrations of, TC, TG, Phospholipids, 2nd and 3rd of atherogenic indices, blood urea, ALP, serum creatinine, LDL - C, AST, Hb, K and MDA compared whith the E G onlay.3 - Induced oxidative stress by H202 caused high significant (P? 0.01) in the concentrations of TC, Phospholipids, 2nd and 3rd of atherogenic indiced blood urea, ALP, serum creatinine, k+, AST, LDL - C, MDA and Hb while significant (P? 0.01) decrease had been happened in the concentrations of albumin, globulin, total protein, HDL - C, GSH, ALT and 1st atherogenic indices.4 - the Treatment rats with (H202)whith lemon juice and parsely for rats produced high significant (P? 0.01) increase in concentrations of albumin, globulin, K+ total protein, HDL - C, GSH, AST. while Increase significant (P? 0.01) in concentrations ofTC, Phospholipids, 3rd of atherogenic indices, blood urea, ALP, serum creatinine, LDL - C, AST, Hb, K and MDA compared whith the(H202) Only.5 - The results of Treatment the animals with Gentamicin and Ciprofloxacin produced high significant (P? 0.01) in the concentrations of TC, TG, Phospholipids, Ist atherogenic indices blood urea, glubulin while the Treatment with Ciprofloxacin caused significant increase (P? 0.01) in concentrations of serum creatinine, VLDL - C, AST, WBCs, MDA, ALT, K+ and Hb.on the another significant decrease had been oecured in the concentrations of ALP, albumin, globulin while decrease of concentrations occurred when the animal had been treated with Gentamicin : total protein, HDL - C, GSH, Hb, Mg+and Ca++.6 - The Treatment by Gentamicin+ Ciprofloxacin+ lemon juice+ parsley to the rats when compares whith rats treated only antibiotics caused high significant (P? 0.01)increase in concentrations of albumin, , globulin, total protein, HDL - C, GSH, K+, Hb and ALP. while high significant decrease happened in the concentrations of total protein, MDA, Ist and3rd atherogenic indices, blood urea, TG, serum creatinine, ALT, MDA, and VLDL - C.7 - The Treatment with Ethylene glycol caused many histological changes like preipration of calcium oxalate in kidney tubules, necrosis, recrudesce of glomerales and caused degredation to the central vein, bleeding in kidney while the two types of antibiotics Gentamicin and Ciprofloxacin caused the same effects as ahave.8 - The action of lemon juice and parsely showed aprotection role against calcium oxalate precipitation and prevented the renal calculi formation or it aggregation in the kidney of the rats treated with Ethylene glycol.the action of that extract and juice were the decrease of oxidative stress damage induced by(H202)in the kidney tissue.

دراسة نسجية فسلجية لتاثيرات خلات اليورانيل والمستخلص المائي لنبات ورد لسان الثور في فعالية الغدة الدرقية في ذكور الارانب البيض == Histological And Physiological Study On The Effects of Uranyl Acetate And Aqueous Extract of Borogo Officinalis On The Activity of Thyroid Gland In Albino Rabbits

Author name: علا صالح علي الجميلي
Supervisor name: كوكب سليم نجم القيسي | منيف صعب احمد ساجت الجنابي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة، للتعرف على تاثير خلات اليورانيل والمستخلص المائي لورد لسان الثور Borago officinalis (Borage) معا وكلا على انفراد في ما ياتي : - 1 التركيب النسجي للغدة الدرقية. - 2 مستوى هرمون T3و T4وTSH. - 3 معايير الدم (التعداد الكلي لخلايا الدم ال | The aim of this study was to determined the effects of uranyl acetate and aqueous extract of Borago officinalis(Borage) alone or when they are given toghther the following : 1 - The histological structures of the thyroid gland.2 - Serum concentration T4, T3, TSH Hormones.3 - The blood parameters (WBC, PCV, Hb, Platelet and ESR). A total 30 mature males(Albino Rabbit) were used in this study, and their weights were recorded before and after the experiment. Animals were divided into five groups and administrated by using oro - gastric tube and included these following groups : 1 - (G1) which was given drinking water and considered as control group. 2 - (G2) group treated with uranyl acetate which was given orally only (50mg/kg body weight) for one month.3 - Group G3 which was treated with uranyl acetate with the same concentration used in (G2), then just after one hour they were post - administrated of with aqueous extract of Borage (adose of 5 ml concentration 10%).4 - (G4) group of animals treated with aqueous extract of Borage and adose of 5 ml concentration 10%.5 - (G5)which was pre - treated with uranyl acetate with concentration similar to the dose in (G2) for after one month, then post - administrated of with aqueous extract of Borage at a dose 5 ml concentration of 10%.At the end of the experiments (one month) blood sample was drawn by heart puncture and then ansethized by using ether, animal were killed and thyroid gland prepare for histological study. The following results were recorded : 1 - A significant decrement (p<0.05) in the mean of animals weight (G2, G3, G5) while there are no significant differences in the (G4)as compared with G1.2 - A significant increment (p<0.05) in the mean of (Hb, PCV) of group G2 where as group G3 showed no significant difference.While group G4 and G5 showed decreased as compared with G1.3 - A significant increment (p<0.05) in the mean of WBC in (G2, G3 and G5) while G4 showed no significant difference as compared G1.4 - A significant increment (p<0, 05) in the (G2, G4 and G5) while G3 there are no significant difference as compared G1.5 - A significant increment (p<0.05) in the mean of platelet in (G2, G3and G4) while there are no significant differences as compared with G1.6 - A significant increment (p<0.05)T4 hormone level, in the (G2, G4) while G5 showed a significant decreased where as G3 showed no significant difference as compared with G1.7 - A significant decrement (p<0.05) in the T3 hormone level in the (G2, G4 and G5) while there are no significant differences in the G3 in comparison with G1.8 - A significant decrement (p<0.05)in TSH hormone level in the G2 while no significant changes in the (G3, G4) and a significant increment in the G5 in comparison with G1. Histological results of thyroid gland 1 - A degenerative changes were observed in the epithelial tissues of thyroid gland in the (G2) due to direct effect of uranyl acetate and loss of cell style aggregation in addition to infiltration with inflammatory cells, with loss of depletion of colloid from thyroid follicles.2 - G3 showed normal thyroid cells and thyroid follicles filled with colloid fluid as compared with control group.3 - The result of histology examination of thyroid of group have shown presence of some follicles that contain colloid while ether lack theis colloid.4 - Characterized thyroid follicles cells (G5) lining simple cuboidal tissue but it contains more than one row of cells, suggesting a hyperplasia, but these effects are less negative compared with (G2).From this study we can concluded that uranyl acetate have negative effect on thyroid cells and the chemical substance of aqueous extract of Borago offcinalis inhibit the negative impact of uranyl acetate on thyroid cells in awide aspects of this study, and the aqueous extract of Borago offcinalis have high effect in regulation of thyroid function

دراسة بعض المؤشرات الوراثية الخلوية لتاثير مادة الكلوروفورم على الفار الابيض Mus musculus L == A Study On Some Cytogenetic Indices For The Effect of Chloroform On The White Mouse Mus Musculus L

Author name: عمر رحيم خلف
Supervisor name: عادل فوزي شهاب
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: يستخدم الكلوروفورم على نطاق واسع في العديد من الصناعات مثل صناعة الورق والمطاط ومطافئ الحريق ومستحضرات التجميل ومواد التخدير. كما يستخدم كمذيب عضوي للعديد من المواد في المختبرات التعليمية والصناعية. وينتج الكلوروفورم من العمليات الايضية التي تقوم بها بعض | Chloroform is widely used in many industries, such as paper, elastic, fire extinguisher, cosmetics and anesthetic materials industries. It also used as an organic dissolvent for many materials in industrial and education laboratories. Chloroform is produced by The metabolism process of living organisms such as macroalgae and some fungus of highly organic matter soil. It can also be produced by process of water cholornation. Because of chloroform is widely used, and few studies achieved on cytogenetic level. Therefore The present study was conducted to identify The effects of chloroform on cytogenetic characteritis in laboratory mice.This study includes : 1 - A Study on Some Cytogenetic Indices For The Effect of Chloroform on The White Mouse.2 - Sperm abnormalities of male mice.The results of The present study showed that The chloroform has the ability to motivate chromosomal aberrations which were represented by (ring chromosom, chromatid break, and centromore break) in somatic cells in both males and females mice and in germ cells in males, The results revealed significant differences in The chromatid break and ring chromosome of both male and females mice, which injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with control group. It was found That there were asignificant differences in centromore break of females, injected with two concentrations of chloroform in comparison with control group, while non significant were differences observed in The males.Also the present study showed there were significant differences in The chromosomal aberration in The germ cells of males mice, injected with two concentrations (0, 025 & 0, 05 mg/kg) when compared with controlled group.The concentration (0, 05 mg/kg) was more influent in comparison, with The concentration (0, 025mg/kg). It was found that 30 days of injection is more influent in comparison with 15 day of injection, and males are more influenced than females.The present study recorded a significant differences in creating micronuclei in both male and female mice, injected by chloroform with a concentrations (0, 025 & 0, 05 mg/kg)in compared with control group.The result also revealed that The chloroform cause a significant decrease in The mitotic index of The somatic cells in both males and females, morever significant decrease in The mitotic germ cells of The injected males mice was found.An increase in The Blast index factor in The somatic cell in both male and female mice, injected by chloroform when compared with control group was similar result was recoded rgarding The Blast Index factor of The males germ cells. Regarding The sperm abnormality, the result showed significant differences in The sperm abnormality of males injected by chloroform with two concentrations (0, 025 & 0, 05 mg/kg) in comparison with The control group. The concentration (0, 05 mg/kg) was more influent than The concentration (0, 025mg/kg). Besides and the period 30 days of injection was more influent than the period 15 days.

دراسة عدد من المعايير الدموية والكيموحيوية وبعض الهرمونات لدى النساء العقيمات في محافظة صلاح الدين == Astudy of A Number of Hematological, Biochemical Parameters And Some Hormones In An Infertile Women In Salahaddin Governorate

Author name: لينا قيس ياسين عبد الرزاق
Supervisor name: زيد محمد مبارك المهداوي | بشرى محمد مجيد
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: هدفت الدراسة الحالية الى تحديد عدد من العوامل المسببة للعقم لدى عدد من النساء في محافظة صلاح الدين. بدات الدراسة من ايلول (2008) ولغاية اب (2009), وحددت نسبة العقم الاولي (%55) والعقم الثانوي (%45) لعينة الدراسة التي شملت 400 عينة عشوائية للنساء المصابات | This study goaled to determine the causes of the infertility in a number of women in Salah - El - Din governorate. The study began in September (2008) until August (2009), the primary infertility was (55%) and the secondary infertility was (45%). The study sample, which included random sample of 400 infertile women aged (15 - 45) years. Whom reviews in Educational Tikrit Hospital and many external women clinics in Tikrit city. The studied samples are compared with 50 healthy women as a controlling group. The age groups of infected women are specified as highest average within (21 - 25) years then from (26 - 30) years then from (31 - 35) years respectively. The study included a cellular classification by using sex chromatin as an indicater of fertile degree (sex chromatin as a guide to classify women by examing Neutrophil polynucleated cells). So wamen were distributed to : Supper female (have 2 sex chromatin), Normal female (have 1sex chromatin) , and Abnormal female (with out sex chromatin).The results showded 4% and 44.75% and 51.25% respectively. Respect to sex chromatin the results revealed high significant levels (P <0.01) and (P <0.05) of prolactin and (LH) in cons. for Abnormal and Normal infertile women. While the same results and levels happened in FSH and Proges. Conc. In Abnormal and Normal infertile women in comparison with the controlling group.this study showed no significant at level (P >0.05) for Estrogen E2 besides there is no significant in comparing infertile women of Supper female with the control group. Concerning biochemical factors and their relation with sex chromatin, it is shown that there is a difference at (P<0.01) (P<0.05) for normal and a bnormal group in comparison with the controlling group, at level LDL, VLDL and total protein between high and low but there is no difference at level HDL, Tri and Chol The study of reproductive hormones (FSH) and (LH) (PRL) and (E2) had been measured at 13 - 14 days of the menstrual cycle of infertile women. And progesterone hormone conc. had been measured at 21 days of the menstrual cycle. The results showded high significant conc. of PRL, LH and Proge. at level (P<0.01) in comparison with control group while their was not significant changes when the infertile women with the control group for FSH and estrogen conc. Respect to the biochemical factors have shown through fat parameter cholesterol, triglyceride, low density lipoprotein - cholesterol very low density lipoprotein - cholesterol and total protein have shown high levels (P<0.01) for infertile women in comparison with control group. While high density lipoprotein - cholesterol and glucose conc. Albumin, conc. and globulin show no significant changes in comparison with the control group. Besides this study has revealed a high levels (P<0.01) at PCV % for normal female in comparison with infertile female but through Hb there is no significant differences. It is also shown that the average of women with poor ovarian is 69.25 % while the block of fallopian tubes line was 18.75% for the endometrosis 4% and unknown infertile female causes were 8%. Then results also show the average of 36% of infertile female who are infected with hyperprolactinemea, results show that they are about 23% who suffer from Polycystic ovary syndrome PCOS. Also the Body Mass Index (BMI) showed high significant level (P<0.01) infertile women compared with the control and the same results happened with the age.

دراسة التاثير المثبط لبعض المستخلصات النباتية على بعض الفطريات المرضية == Study of The Inhibitory Effect of Some Medicinal Plants Extracts On Some Pathogenic Fungi

Author name: شفاء طيار جعفر العساف
Supervisor name: صالح عيسى محمد الجبوري | عبد الكريم سليمان حسن النعيمي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اظهرت دراسة التاثير المثبط للمستخلصات المائية والكحولية لاربعة نباتات طبية هي النعناع بنوعيه (Horsemint, Spearmint) والسعد (Nutgrass) والحبة السوداء(Black Cumin) على بعض الفطريات الممرضة المعزولة من حالات مرضية مختلفة، من بين (63) عينة مرضية اخذت للدراسة | Study of inhibitory effect of aqueous and alcoholic extracts of four medicinal plants, spearmint, horsemint, nutgrass and black seed (black cumin), against some pathogenic fungi isolated from different clinical cases, showed that (12) cases from (63) cases were positive and Candida albicans was more frequent (41.67%) followed by Aspergillus fumigates and A. niger (16.67%) for each one.The study at inhibitory effect of aqueous extracts against the isolated fungi, showed that aqueous extract of black seed (30 mg/ml.) was more effective, and inhibited growth of the fungus A. fumigatus (87.5%) followed by the aqueous extract of black seed (25 mg/ml.), nutgrass and Spearmint (30 mg/ml.) for both, which inhibit the fungal growth (81.25%).Aqueous extract of Horsemint (30 mg/ml.) was the best one, which inhibited the growth of A. niger completely (100%); Aqueous extract of black seed (30 mg/ml.) was more effective against the fungus candida albicans, and the inhibition zone diameter was (13 mm.).The study of inhibitory effect of alcoholic extracts against the isolated fungi, showed that alcoholic extracts of black seed (10 mg/ml.), Spearmint (15 mg/ml.) and Horsemint (30 mg/ml.) were The best against A. fumigatus and inhibited its growth completely (100%).Alcoholic extracts of black seed (15 mg/ml.), Spearmint (20 mg/ml.) were more effective against A. niger, and inhibited its growth completely (100 %); Finally, alcoholic extract of black seed (30 mg/ml.) was the best one against C. albicans, with inhibition zone diammeter (19 mm.).Testing of inhibitory effect of some chemical antifungal agents against the isolated fungi showed ketoconazole at concentrations (3.5, 3.0, 2.5) mg/ml. inhibited the growth of A. fumigatus (100, 93.0, 90.0) % respectively. Also ketoconazole at concentrations (3.5, 3.0, 2.5) mg/ml. inhibited the growth of A. niger (100, 95.0, (93.0)% respectively. Nystatin; at concentration 100 I.U/Disc (0.06 mg/Disc) inhibited growth of C. albicans with inhibition zone diammeter (15 mm.).

دراسة عدد من المعايير الكيموحيوية والاجهاد التاكسدي لدى المرضى المصابين ببعض امراض القلب == The Study of Some of Biochemical Criteria And Oxidative Stress In Patients With Certain Heart Diseases

Author name: منى احمـد لفتة النيسانـي
Supervisor name: زيد محمد مبارك المهداوي | موسى جاسم محمد الحميش
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: صممت الدراسة الحالية لمعرفة تاثير الاجهاد التاكسدي لدى المرضى المصابين ببعض امراض القلب عن طريق اجراء بعض الاختبارات والقياسات كالنسبة المئوية لحجم كريات الدم المرصوصة (PCV) وتركيز خضاب الدم (Hb)، وبعض الانزيمات مثل : تركيز انزيم ناقل امين الاسبارتيت (AST | The current study was designed to determine the effect of oxidative stress in patients with the certain heart diseases through some tests and measurements as the Packed Cells Volume Percentage (PCV) and hemoglobin concentration (Hb), and some enzymes as : the Concentration of Enzyme Aspartate Aminotransferase (AST), Creatine Phospho Kinase (CPK), Total Lactate Dehydrogenase (LDH), and Lactate Dehydrogenase For Heart Muscle, and some of antioxidants and oxidation factors as : Glutathione (GSH), and Malondialdehyde (MDA) respectively, and lipid profile that include : the Concentration of Total Cholesterol, Triglycerides (TG), High Density Lipoprotein of Cholesterol (HDL - C), Low Density Lipoprotein of Cholesterol (LDL - C), and Very Low Density Lipoprotein of Cholesterol (VLDL - C), and some other biochemical criteria as : the Concentration of Glucose, Total Protein, Albumin, Globulin, Urea, and Creatinine. It was chosen (95) blood sample back to the (80 patients and 15 healthy people), the samples were divided into four groups : The first group : included patients with Myocardial Infarction and they was (40) patients (males and females), age ranged between (35 - 89) years old.The second group : included patients with Angina Pectoris and they was (20) patients (males and females), age ranged between (32 - 85) years old.The third group : included patients with Heart Failure and they was (20) patients (males and females), age ranged between (39 - 89) years old.The control group : included (15) people (males and females), age ranged between (30 - 57) years old. The current study showed the following results : A. A significant decrease in the level (p <0.05) in : 1 - The Packed Cells Volume Percentage (PCV) and the concentration of Hemoglobin (Hb) in the heart failure group within the group of (males+females) and females gender.2 - Concentration of Glutathione (GSH) in the three pathological groups within the groups of males and females and (males+females) and age groups (60 years old and above).3 - Concentration of (HDL - C) in the three pathological groups for males and (males+females).4 - Concentration of Total protein in the myocardial infarction group for males and in the heart failure group for females and these two groups within the group of (males+females) and smokers.5 - Concentration of Albumin in the myocardial infarction and heart failure groups for males and (males+females).6 - Concentration of Globulin in the heart failure group for females and (males+females).B. A Significant increase in the level (p <0.05) in : 1 - Concentration of Enzyme Aspartate Aminotransferase (AST) in the angina pectoris group for males and the heart failure group for females and these two groups within the group of (males+females), males gender, diabetes and smokers.2 - Concentration of Enzyme Creatine Phospho Kinase (CPK) in patients with myocardial infarction for males and female and (males+females), males gender, age group (80 - 89 years old), people with high blood pressure, diabetes and smokers.3 - Concentration of Enzyme Total Lactate Dehydrogenase (LDH) and Lactate Dehydrogenase For Heart Muscle in the myocardial infarction group for males and females and (males+females), males gender, age group (50 - 59 years old), diabetes and smokers.4 - Concentration of Malondialdehyde (MDA) in the heart failure group for males and females and (males+females) and females gender.5 - Concentration of Total Cholesterol in a group of angina pectoris for males and females and (males+females).6 - Concentration of Triglycerides (TG) in the myocardial infarction group for females and (males+females), males gender, diabetes and smokers.7 - Concentration of (VLDL - C) in the myocardial infarction group for males and females and (males+females), males gender, diabetes and smokers.8 - Concentration of (LDL - C) in the angina pectoris and heart failure groups for males and females and (males+females).9 - Concentration of Glucose in the heart failure group for males and myocardial infarction group for females and these two groups within the group of (males+females), males gender, age groups (50 - 79 years old), diabetes and smokers.10 - Concentration of Urea in the myocardial infarction and heart failure groups for males and females and (males+females) and age groups (from 60 - 79 years old).11 - Concentration of Creatinine in a myocardial infarction group for males and females and (males+females) and age group (70 - 79 years old).

دراسة تاثير النفط الخام وهايدروكسيد الامونيوم على بعض صفات الترب ونمو نبات الشعير الابيض == Study The Effect of Crude Oil And Ammonium Hydroxide On Some Characteristics of Soils And Plant Growth of Barley White

Author name: براء محمد ابراهيم
Supervisor name: جهاد ذياب محل الجنابي
General topic: Biology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: شمل البحث دراسة انتشار المخلفات النفطية وهايدروكسيد الامونيوم في مقاطع لاربعة ترب مختلفة النسجة وهي التربة (المزيجية الرملية، المزيجية الغرينية، المزيجية الطينية 1، والمزيجية الطينية 2)؛ اجريت التجربة باستخدام صندوق مصنوع من الخشب الغير منفذ للماء؛ اذ قسم | Research involved studying the spread of oil residues and hydroxide ammonium in excerpts of four soils of different Texture, the soil (sand, gypsum, Loam, and clay); experiment was carried out using a box made of wood and non - port of the water; it was divided into four sections with barriers, wooden supported tin galvanized to ensure that Waste oil spread between the different types of soils, left central Hole made of tin and galvanized perforated and lined with container made of filter paper. Container filled with waste oil and allowed to seep into sections of the four soils through the holes, where waste oil was allowed to spread for 45 days, after which a series of analyzes were conducted to look for oil residue in the soil sections of the experiment. Then added ammonium hydroxide liquid substance in the central Jura and left for 45 days also were conducted after a series of analyzes to determine the changes and the effects on the soil material and components; was also white barley cultivation, After two months of growth, a set of analyzes were conducted Hordeun vulgare Barley and whose scientific name to find out the impact of oil on the chemical characteristics and uptake of heavy metals.Results were recorded the highest prevalence rate of the waste by 218.5 ppm in the Loam soil in depth 15 cm, while the lowest in the clay soil at the depth of 45 cm 44.8 ppm, while in the sandy soil did not happen the spread in the depths of 15 cm and 30 cm; also recorded the highest connectivity Power in the Loam soil 5.37 Micro Smenz / cm and recorded clay soil less connectivity, electric 1.81 Micro Smenz / cm; and when measuring the pH was recorded sandy soil 8.3, while they recorded the soil gypsum 7.5 As for the relative humidity in the soil recorded soil Loam the highest percentage is 93.55% in while the lowest percentage recorded in the soil moisture gypsum 57.90%. Were measured concentration of heavy metals such as cobalt, where the highest concentration in sandy soils 15.40 ppm while the lowest concentration him in the clay soil 7.10 ppm; and cadmium, where the highest concentration in soil gypsum 58.10 ppm in return was the lowest concentration of cadmium 14.60 part million in the clay soil; and when measured the highest concentration of lead found in the soil has Loam reaching 122.80 ppm was the lowest concentration of lead in soil clay 71.90 ppm.Reached the highest value of chlorophyll a and chlorophyll b in plants grown in clay soils (3.21, 2.96) Eq ml / g, respectively, while the highest concentration of phosphorus, chlorine, magnesium, sodium, calcium, and potassium in the soil clay (5.6, 15.9, 2.9, 16.4, 20.1, 46) ppm, respectively;As for the heavy elements (chromium, cobalt, cadmium, lead, and iron) have the highest concentrations in plants grown in soil gypsum, as was (0.98, 0.24, 0.70, 0.31, 14) ppm.While the lowest value for chlorophyll a and chlorophyll b in plants grown in sandy soil (1.62, 1.24) Eq ml / g, while the lowest concentration of elements (phosphorus, chlorine, magnesium, sodium, calcium and potassium) in plants grown in sandy soil reaching (4.2, 4.1, 1.2, 5.9, 1.2, 12.2) ppm, the lowest concentrations of heavy elements were recorded in the sandy soil as well as the (0.03, 0.12, 0.20, 0.20, 8.3) ppm.

دور الفئران المنزلية Mus musculus والجرذان النرويجية Rattus norvegicus في وبائية بعض الامراض الطفيلية من اوالي الدم والانسجة في مدينة تكريت وضواحيها == The Role of Mus Musculus And Rattus Norvegicus In The Epidemiology of Some Blood And Tissues Parasite Diseases of Protozoa In Tikrit City And It'S Districts

Author name: شيلان قادر صادق الصالحي
Supervisor name: توفيق ابراهيم محمد الالوسي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت الدراسة في مناطق وبعض اقضية محافظة صلاح الدين للفترة من كانون الاول 2006 الى الفترة تشرين الثاني 2007، وتم فحص 200 نموذجا من الفئران المنزلية Mus musculus و50 نموذجا من الجرذان النرويجية Rattus norvegicus جمعت من ثماني مناطق وهي حي شيشين وحي الجمعية | The study was carried out in various regions of Selah El - Deen from the period 12 - 2006 to 11 - 2007. In this research a total of two hundred Mus musculs and fifty Rattus norvegicus from eight regions of Selah El - Deen area, namely, Sheshen area, Al - Jamiea area, Al - Sikak area, Al - Kadisea, Al - Alban Factory, Al - Dor, Samara and Al - Alam were investigated. The examination results of the brain, liver, spleen and blood smears of each rodent were showed 4 species of parasitic protozoa : 1. Toxoplasma gondii : This species found in Mus musculs only with the percentage of infection (2.0%).2. Leishmania spp. : This species found in Rattus norvegicus only with the percentage of infection (2.0%).3. Eperythrozoon coccoides : This species found in the blood of Mus musculs only with the percentage of infection (1.5%).4. Encephalitozoon cuniculi : This species found in Mus musculs with the percentage of infection (3.0%), and in Rattus norvegicus with the percentage of infection (4.0%).The percentage of infection in Mus musculs was (6.5%) but the percentage of infection in Rattus norvegicus was (6.0%).Encephalitozoon cuniculi appeared in a high percentage of infection in both Mus musculus and Rattus norvegicus.The experimental results of heart and lungs tissues for all rodents studied was negative. The statistic results showed no significant difference in percentage of infection among species of protozoa in Rattus norvegicus, but there were significant difference in percentage of infection among species of protozoa in Mus musculus.There were no significant difference in percentage of infection between collection area of Rattus norvegicus, but there were significant difference in percentage between collection regions of Mus musculus.The seasons of year had no significant difference on the percentage of infection with Rattus norvegicus, but there were significant difference in percentage between the seasons of year in Mus musculus.Rodents sex showed no significant difference in percentage of infection in Mus musculus and Rattus norvegicus

دراسة تاثير مستخلصات القرفة (Cinnamomum zeylanicum) والسماق (Rhus coriaria) في التثبيط المايكروبي وبعض المعايير الحيوية في ذكور الجرذان == Study The Effects of Cinnamon (Cinnamomum Zeylanicum) And Sumac (Rhus Coriaria) Extracts On Microbial Inhibition And Some Biological Parameters In Rats Male

Author name: فراس عدنان حسين الزيدي
Supervisor name: كركز محمد ثلج الجبوري
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: صممت الدراسة في مختبرات قسم علوم الحياة / كلية التربية وقسم علوم الاغذية / كلية الزراعة في جامعة تكريت, اذ تضمنت الدراسة التعرف على المواد الفعالة في المستخلصات المائية والكحولية في كل من قلف القرفة وبذور السماق واختبار فعاليتها التثبيطية عند تركيز 10, 25 | This study was conducted in Biology department laboratory / Education college and Food science department in Agriculture college in Tikrit University. the study was conducted to investigated to the aquaies and alcoholic activities extracts from each Cinnamon bark and sumac seeds and assay of inhibition activity concentration at 10, 25, 50 and 100 mg/wells against gram positive and negative bacteria and phagocytosis Immunity level after injection by E.coli type, further more to study the effect it in some physiological parameters in male rats after oral administration for 8 days by 50 and 100 mg/kg from rats body weight from each aquais and alcoholic extracts. The results was illustrated the aquaies and alcoholic extracts from each Cinnamon bark and sumac seeds were contains the active groups from compound such as Tannins, Resins, Phenols, Flavonoids, Alkaloids, Glycosides, Coumarins. while the effects was found the aquaies extracts a high significantly effects (p < 0.05) from alcoholic by inhibition ability when assay. The Minimum inhibition concentration (MIC) from each ones which mass 50 and 125 mg/ml from culture media to inhibition concentration by E.coli and S.pyogen cinnamon bark aquaies extract. it was 75 and 100 mg/ml from culture media to inhibition concentration by E.coli and S.pyogen from alcohol extracting its inhibiting efficiency throw the measure of the diameter of the inhibiting area that has shown that the gratest effect was E.coli bacteria which its diameter of inhibiting area 17 mm and when the concentration is 100 mg/wells from the alcoholic and aquaies extracting and the less effect is S.pyogen and S.aeruginosa in case of the extraction from cinnamon bark it was 17 mm the diameter of inhibiting area of E.coli bacteria. when the concentration 100 mg/wells from aquaies extracting from sumac. whether with alcohol extraction, the bacterial type were similar in their sensitivity in diameter of inhibiting from 18 to 20 mm but the oral administration of the extract on the level of phagocytosis process through the injection of rats in affixed number from E.coli bacteria. The result was illustrated the type of concentration from each cinnamon bark and sumac seeds such as aquaies and alcoholic was caused an increase in the phagocytosis process through the significantly decrease in the number of bacteria with increase of the concentration from the groups that oral administration from extraction that there number wear (zero) on the concentration 100 mg/kg from rats body weight when the time is 120 minute which was equal with effincy of the ciprofloxacin antibiotic on the concentration 5 mg/kg from rats body weight. The result also showed the effect of oral administration from the extraction of cinnamon bark and sumac seeds such as aquaies and alcoholic, that significantly caused the decreased in the concentration of the hemoglobin and Total count red blood cells and the packed cell volume on the concentration 100 mg/kg from the weight but the measures have not significantly changed the parameters MCV, MCH, MCHC with increase of concentration from extraction compared with the group of controlling. The white blood cells also decreased in the significantly aquaeis and alcoholic extracting from the cinnamon bark it was 6.8 × 103/mm3 on the oral administration of the rats from the concentration 100 mg/kg from the body weight of the aquaeis extracting, and 6.8 × 103/mm3 on the oral administration of the rats from the concentration 100 mg/kg from the body weight of the alcoholic extracting, That numbers have not affected during the oral administration from the extractions of sumac only on the concentration 100 mg/kg from the body weight of the alcohol extracting which was 5.6 × 103/mm3 the increase was in the ratio of the number of the neutrophils in the whole extraction alcoholic and aquaies for the both of plants. While the effect of oral administration from the extraction in the lipids profiles was clear in the decrease significantly in the concentration of the glucose and albumin and Urea and uric acid and creatinine and triglyceride and, cholesterol, and the low density lipoproteins (LDL) and very low density lipoproteins (VLDL), significant increasing in globulin and high density lipoproteins (HDL) in aquaies and alcoholic extraction of sumac seeds while the value of Total protein and HDL is not significantly differtited in aquaies and alcoholic extraction moreover oral administration of the both of plants. The enzyme activity value was included significantly decreased of the Alkaline phosphtase activity value with the increased orally administration from aquaies and alcoholic extracts from both of plants, while increased significantly Aspartames amenotransferase (AST) activity value in all cinnamon extracts while was significantly decreased when administrate from sumac seeds extract, But don’t significantly differ Alanine amenotransferase (ALT) activity value in all concentration from aquaies and alcoholic extracts from both of plants.

انتشار القراد الصلب Ixodidae : Acari وعلاقته مع بعض الاوالي السبورية في بعض المناطق المحيطة بمدينة تكريت == Bovine'S Hard Ticks (Acari : Ixodidae) And Their Relation With Some Haemosporian Parasites In Tikrit Rural Boundaries

Author name: الاء عماد توفيق التكريتي
Supervisor name: توفيق ابراهيم محمد الالوسي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تناولت الدراسة الحالية مسحا للقــراد الصلب المتطفل على الابقار في بعض المناطق المحيطة بمركز مدينة تكريت وللفترة من 1/10/2010 ولغاية 30/7/2011، وقد جمعت العينات من مناطق العلم والبو عجيل وعوينات والمحزم. اختير لاتمام هذه الدراسة مضيف واحد هو الابقار لما له | Ectoparasitic Ioxdae (hard ticks) of cattle were surveyed from five rural stations near by Tikrit city/Iraq since 1st. October 2010Till 30 th. Jully 2011. The sampling stations as fellows : Alam , Albo - Ajeel, Ewanat and al - Mahzam.It he neer chosen only oneHost which is caw.The tick speciemens were collected from one to foure - year cattle host with both sexes. It was found that theHost was infested with two ixodid species ; Hyalomma anatolicum anatolicum and Boophilus annulatus. H. anatolicum anatolicum was the dominant one in all the survey stations.It has been recorded in this study which carry for the first timprovince of Salah EL - Din on tick and disease which is transmittd. The species of Hyalomma anatolicum represent a high percent of infection in the province. As well as the present study showed the presence of relation between temperature and infection by ticks. This study showed that the ticks were aparasitic on ears, nipples and percenal regoin.In comparison between (67.16 %) to (47.82%) for Alam and Al - Mahzam respectively. The presence study included also Exploring the parasitic disease transmitted by tick, That shown infection of cows by three type of parasite which are Theileria, Babesia and Anaplasma from both sex. where Theileria recorderd the high percent (54.65 %) fllowed by Anaplasma ( 23.97 %) and last percent Babesia (13.77 %). while mixed infection the ratios were (50.68 %, 34.24 %, 15.06 %) respectively.

دراسة مظهرية وتشريحية مقارنة لانواع في اجناس معينة من العائلة المركبة Compositae (Asteraceae) في العراق == A Comparative Morphological And Anatomical Study of Species In Certain Genera From The Family Compositae (Asteraceae) In Iraq

Author name: عمـر خليل جاسـم محمد العباسي
Supervisor name: خليل ابراهيم عباس الشمري
General topic: Biology
Specific topic: Plant
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تناولت الدراسة الحالية, دراسة مظهرية وتشريحية مقارنة لـ(10) انواع تعود لـ(9) اجناس من العائلة المركبة Asteraceae (Compositae) في العراق, كما وتبعت الاجناس قيد الدراسة الى عشيرتين Tribes : العشيرة الاولى : (Cardueae), وقد ضمت الانواع : (Arctium tomentosum, Ca | The current study dealt with the morphological and anatomical aspect that compared the (10) Species that followed (9) genera, These Species followed the family Compositae (Asteraceae) in Iraq. It also divided the genera under study to two tribes. The first tribe : (Cardueae), It included the Species : (Arctium tomentosum, Carlina vulgaris, Chardinia orientales, Cnicus benedictus and Notobasis syriaca). The second tribe : (Lactuceae), It included the Species : (Geropogon glabrum, Geropogon hybridus, Hedypnois cretica, Koelpinia linearis and Urospermum pecroides). The morphological study involved the roots according to the characteristics, the form and dimensions (the length and the diameter), the study of the stem by the character, the color and the indumentums, also the dimension (the high and diameter) it resulted good characteristics, enabled us to use it in isolation of the types. It is also discussed the leaf on the based of the quantifier and qualifier that involved the form of the leaf and the form of the peak and the base, the character of the blade margin and the epidermis. The study also high lighted the dimension of the blade (the length and the width), The morphological characteristics of the leaves have given a great Taxonomical value rather than the root and the stem. It also mentioned in this study the Inflorescences in general : the form and the diameter of the receptacle, the dimension of the inflorescence (length and width), and the characteristics of the phyllaries, the study of Achenes, the length of the beak, the character of the filaments that connected by the peak of the beak. The proper morphological characters of the Inflorescences made the contrastive among the characteristics of the Inflorescence in unique type. This great advantage is useful in the classification of the species. The anatomical study contains the study of the dermal tissue system that belong to the types under study, specially the stem, the leaf. It make clear the differences among the characters of the epidermis cells for the stem and the leaf (the upper and lower epidermises), It tackled the study of the Stomata at the leaf and the stem, and measure of the frequency at these members. It also discussed the fundamental and vascular tissue system of the root, the stem and the leaf through the study of their characteristics of the transversal sector. It is also studied the vascular bundles according to the thickness of the regions belonged to the xylem and phloem; the number of the xylem arms in each bundle and the number of the vascular elements in one xylem arm. The study involves the system of the veins into the leaf blade or that called (Venation), and the thickness of the veins and its course, the behavior of the vein's ends and the method of its contact with the leaf margin. In addition, the study dealt with the method of the joining veins the secondary by the primary and the third veins by the secondary. Thus, the study make clear the considerable advantages that distinguish the types each other. It is observed the characteristics of the leaf's venation is a constant character that depends upon to classify the species. At end of the study, It is used the results of the morphological and anatomical characteristics in comparison of the two tribes; Cardueae and Lactuceae that the studied species followed.

علاقة مستوى الزنك والنحاس ببعض المعايير المناعية في الاصابة بداء المقوسات في النساء في محافظة كركوك مع محاولة علاجية في الفئران المختبرية == Relation of Zinc And Copper Levels With Some Immunological Markers In Women With Toxoplamosis In Kirkuk Governorate With Trial To Treatment In Mice

Author name: عبير عباس علي
Supervisor name: حسين ساهر اسود العبيدي | شهاب احمد محمد
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تم دراسة 100امراة مصابة بداء المقوسات Toxoplasmosis الذي يسببه الطفيلي المقوسة الكوندية Toxoplasma gondii للفترة من اذار 2007 ولغاية نيسان 2008 راجعن العيادة الاستشارية التابعة لمستشفى كركوك العام في مدينة كركوك. اظهرت نتائج الدراسة الحالية ان 56 % من الن | A total of 100 patients suffering from Toxoplasmosis which is infected by the parasite Toxoplasma gondii have been dealt with as subject of the sample during the period from March 2007 to April 2008 at the Consultant Clinic of Kirkuk General Hospital. The results of the present study showed that 56% of the subjects were pregnant and 44%were not. The highest percentage of the infected subjects was of 26 - 30 years old as 30.37% among the pregnant women and 36.35% among the non - pregnant women of the age mentioned above. The highest percentage 37.5% of abortion of the first period of pregnancy was in pregnant women, and 45.53% in non - pregnant women. The results of the study also showed more cases of infected women among those who bred animals in their living places as 69%. It is also found that the highest percentage of the infected women 64% were of those who use the running water for drinking. Moreover, it is noticed that there is an increase in the percentage of the infected women among the uneducated women 38%. The study showed that the percentage of the infection is proportional to residence 63%, the infection increase among women living in rural areas. The percentage of T - lymphocytes in peripheral blood of patients were also measured through measuring the percentage of the CD4+ and CD8+cells by using fluorescent microscope.The results showed a significant decrease (p<0.05) in this type of cells.The rate of percentage of CD4+ of the patient's were 31.704±0.116 while the rate of CD4+ in the peripheral blood of the control group was 62.260±0.237.The rate of percentages of CD8+ 19.382±0.080, whereas it was 31.680±0.307 of the control group.The rates of the percentages of CD4+ to CD8+ were also measured, the results showed a significant decrease (p<0.05) in the rate of the percentage in the patient's group 1.642 while it was 1.968 in the control group. The statistic analysis showed significant differences between these tow groups (p<0.05). The intensity of Zinc and Copper has been measured in the serum of the infected women via use of Atomic absorption instrument, a significant decrease (p <0.05) appeared in the rate of Zinc intensity in the serum on the infected women as compared with the control group.The rate of the intensity in patient's group was 4.65% ± 2.628µmol /l while in the control group it was 15.353± 1.640 µmol /l.A significant increase (p<0.05)was also noticed in the intensity of Copper in the patient's group (28.589±4.335 µmol /l)whereas it was 12.854±1.501 µmol /l in the control group. The index of the specific antibodies IgM and IgG related to Toxoplasma gondii was measured by ELISA method, a significant increase (p<0.05) was observed in the value of IgM and IgG in the patient's group as compared with the control group. The rate of index for IgM in the patient's group was 1.354±0.05 while it was 0.163±0.05 in the control group. The rate of value of IgG in the patient's group was 1.163±0.074 whereas it was 0.358±0.033 in the control group. In the present study, Toxoplasma gondii was isolated and diagnosed from human as first attempt, and then the infection was created in laboratory mice as an essential step for growing the parasite. The second step was studying the effect of some selected drugs on the experimental treatment for disease of toxoplasmosis created in the mice. The drugs are : Pyrimethamin, Clarithromycin, Spiramycin, Ivermectin.The results of the study showed the effects of these drugs on the tissue cyst formed in the brain and lungs. The number of tissue cysts in the brain of the mouse treated with pyrimethamin was 14.8 ±1.067 after 28 days. Significant differences appeared when this result compared with the control group in which it was 27.4±1.029. The number of tissue cysts formed in lung, tissue in the mouse treated with the same drug after the same period of the time was 6.2±0.583.This showed a significant differences as compared with the control group in which it was 10.2±0.583. No significant differences appeared between both groups as for the treatment by Clarithromycin antibiotic as far as the number of the tissue cysts formed in the brain after 28 days is concerned. The number was 23.8 ±0.86 as compared with the control group 38.6±1.029.The same compared is true of the lungs, tissue. The number of the tissue cysts was 23.8±0.86 as compared with the control group 28.4±1.805. Concerning the antibiotic Spiramycin, the results showed the effectiveness of it in decreasing the tissue cysts formed in the brain.The significant differences were (p<0.05), the rate of the cysts was 9.6±0.6 as compared with the control group in which it was 22.8±1.067.The same effectiveness appeared on the lungs, tissue as the rate of tissue cysts after 28 days of treatment was 4.6±0.509 as compared with the control group in which was 17±1.14 and the significant differences were (p=0.000).The Ivermectin drug showed a great effectiveness in minimizing the number of the tissue cysts formed in the brain and the lungs. The numbers of the tissue cysts formed after 28 days were 3.2±0.374 and 1±0.316 respectively; significant differences are observed when these numbers are compared with the numbers of the control group which were 22±1.843 and 19.4±1.435. Thus, the use of Ivermectin drug is considered as a pioneer and fruitful study since this drug has not been used for the treatment of Toxoplasmosis.

دراسة بعض مظاهر امراضية الخمج بالمتحولة النسيجية بين الاشخاص في مدينة سامراء == A Stud Y of Some Aspects of Entamoeba Histolytica Pathogenicity Among Peoples In Samarra

Author name: صلاح عبد حسن السامرائي
Supervisor name: ابراهيم شعبان داوود | حسين ساهر اسود
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: شملت هذه الدراسة فحص (540) عينة براز من المصابين بالاسهال والمراجعين لمستشفى سامراء العام وذلك خلال الفترة الممتدة بين 1/10/2007الى 1/6/2008 فحصت العينات بطريقة المسحة المباشرة المزدوجة للكشف عن الاطوار الخضرية والمتكيسة لطفيلي اميبا الزحار. واظهرت النتائ | The current study dealt with examination of (540) stool samples for patients with diarrhea who visited the General Hospital of Samarra during 1/10/2007 - 1/6/2008. The samples were examined by double wet direct smear method to identify the trophozoite and cyst stages of Entamoeba histolytica. The results showed that (112) were infected with amoebic dysentery. The total rate was (20.74 %). (262) are males with a rate of infection (19.84 %), whereas, the number of females was (278) with an infection rate (21.58 %). The study showed an increase rate of infection among the rural population, it was (25.71 %), whereas the urban population rate was (16.6 %). The study covered all ages beginning from less than a year to 75 years old. A highest rate of infection was recorded by (10 - 19) years old, it was (29.4%). The study also, found out that a highest rate of infection was during school months, particularly during November (27.5 %). Examinations of complete blood picture which included Hemoglobin concentration (Hb), packed cells volume (PCV), white blood cells count (WBCc), and erythrocyte sedimentation rate (ESR) were done on all patients infected with amoebic dysentery. The results were compared with those of healthy ones (Control Group). The results showed significant differences between the two groups. According to the significant one (P < 0.05), it recorded a decrease (16.9%) in the (PCV) and (25.1%) in the (Hb) and an increase (241.8%) in the (ESR) and (51.5%) in the (WBCc) for those ones infected with amoebic dysentery if compared with the control group. Clinical biochemistry examinations were performed, on blood sugar (Bs), electrolytes such as Sodium (Na+), Potassium (K+), Calcium (Ca++), Magnesium (Mg++), liver functions tests : total serum bilirubine(TSB), tranceaminase enzyme(GOT, GPT), alkaline phosphetase (AlP), total protein (TP) and albumin (Alb). The results showed that there are significant differences between persons infected with amoebic dysentery and the healthy ones according to the significant level (P < 0.05) in these examinations. The study showed a decrease(21.6%) in the (Bs) mean concentration, (16.8%) in the(K+) mean concentration, (5.8%) in the(Ca++) mean concentration, (4.5%)in the (Mg++) mean concentration, (10.5 %) in the (Na+) mean concentration , (17.2%) in the (TP) mean concentration and(12.2%) in the (Alb) mean concentration and increase (83.7%) in the (Alp) mean concentration, (52.1%) in the(TSB) mean concentration, (81.1%)in the (GPT) mean concentration, (59.9%)in the (GOT) mean concentration and(22.8%) in the (globulin) mean concentration in the persons infected with amoebic dysentery compared with the mean concentrations in healthy ones control group.

دراسة تاثير المستخلص المائي لنبات اللهانة على مستويات هرمونات الدرقية, الاجهاد التاكسدي وعدد من المعايير البيولوجية في الارانب == Study The Effect of Cabbage Aqueous Extract On The Thyroid Hormones Levels, Oxidative Stress And Some Biological Parameters In Rabbits

Author name: سرى سمير محمد الدوري
Supervisor name: زيد محمد مبارك المهداوي | صاحب جمعة عبد الرحمن
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: هدفت الدراسة الحالية الى معرفة بعض تاثيرات المستخلص المائي لنبات اللهانة ((Brassica oleracea على مستويات هرمونات الغدة الدرقية الثايرونين ثلاثي اليود Triiodothyronine (T3) والثايروكسين (Thyroxine (T4والهرمون المحفز للدرقية Thyroid stimulating hormone (TSH | The aim of the present study was to investigate some effects of the aqueous extract of cabbage (Brassica oleracea L) on the Thyroid hormones concentrations Triiodothyronine T3, Thyroxine T4 and Thyroid stimulating hormone (TSH), Body weight rate, and some physiological and biochemical parameters such as the total count of leukocytes (WBCs), packed cell volume (PCV) and concentrations of Hemoglobin (Hb), Glucose, Total Cholesterol(TC), Triglycerides (TG), High Density Lipoprotein (HDL - C), Low Density Lipoprotein (LDL - C), Very Low Density Lipoprotein (VLDL - C), Phospholipids, Atherogenic indices levels, Uric acid, Total protein, Albumin, Globulin, Serum Creatinine Blood urea, glutathione(GSH), Malondialdehyde(MDA), Peroxynitrite radical(ONOO - ) Ceruloplasmin(Cp), Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) in sera of male rabbits exposed to oxidative stress induced by hydrogen peroxide (0.5%) with drinking water along the period of experiment (30) days, with comparison the effect of the cabbage extract with the effect of carbimazole (anti - thyroid drug) to induce Hypothyroidism.For the purpose of this study, preliminary experiments were carried out to find the most effective dose of the cabbage extract and this dose was (300 mg/kg body weight).Rabbits (8 - 10 months age) and (950 - 1560 gm weight) were determined randomly divided into five groups, each group includes five replicates These groups are : The control group, cabbage aqueous extract group, cabbage aqueous extract and H2O2 group, carbimazole drug group and H2O2 GroupThe results showed the following : 1 - Treatment the animals with cabbage aqueous extract caused significant decrease (P<0.05) in Hb, PCV and concentrations of T3 hormone, T4 hormone, TC, LDL - C, phospholipids, second atherogenic index, blood urea, serum creatinine, albumin, MDA, (ONOO - ) radical and Cp, While it caused significant increase in weights, TSH hormone, TG, VLDL - C, glucose, globulin and GSH compared with the control group. While no significant variations were observed in total (WBCs) and concentrations of AST, ALT, HDL - C, atherogenic index (first and third), uric acid and total protein compared with the control group. 2 - Treatment of rabbits with carbimazole drug showed significant decrease in levels of body weight, Hb, PCV, and concentrations of T3 hormone, HDL - C, blood urea, albumin, glucose, GSH and Cp. This treatment showed as well a significant increase in T4 hormone, TSH hormone, ALT, TG, uric acid, VLDL - C, serum creatinine and atherogenic indices levels, Whereas showed no significant variations in total (WBCs), AST, TC, LDL - C, phospholipids, total protein, globulin, MDA and (ONOO - ) radical compared with the control group. 3 - Compared with the control group the oxidative stress induced by H2O2 caused significant decrease in concentrations of Hb, T3 hormone, HDL - C, albumin, GSH and Cp, While this stress showed a significant increase in total (WBCs), T4 hormone, ALT, TG, VLDL - C, phospho - lipids, atherogenic indices levels, serum creatinine, glucose, MDA and (ONOO - ) radical. However no significant variations were observed in weights, TSH hormone, PCV, AST, TC, LDL - C, uric acid, blood urea, total protein and globulin compared with the control group.4 - The Treatment of rabbits under oxidative stress with cabbage extract showed significant decrease in Hb, PCV, T3 hormone, TC, LDL - C, phospholipids, atherogenic indices levels, blood urea, serum creatinine, albumin, GSH, MDA, (ONOO - ) radical and Cp comparison with the control group. While a significant increase was observed in weights and concentrations of T4 hormone, TG, HDL - C, VLDL - C, uric acid, glucose and globulin, While no significant variations showed in total (WBCs), TSH hormone, ALT, AST and total protein comparison with the control group.The study results revealed that the effect of cabbage aqueous extract was better than carbimazole effect as antithyroid and as antioxidant role by reduction the harmful effects of the free radicals through some improvement was observed in many physiological and biochemical activities in the body and may be used in protection and treatment many of pathological cases specially Hyperthyroidism, cancer and cardiovascular diseases.

التاثير الوقائي لعصير التفاح الاخضر والمستخلص المائي للزنجبيل في التقليل من التاثيرات الفسلجية والكيموحيوية للاشعة فوق البنفسجية والاشعة السينية في الجرذان == The Protective Effect of Green Apple Juice And Ginger Aqueous Extract In Lowering of The Physiological And Biochemical Effect From Ultraviolet Ray And X - Ray In Rats

Author name: عبير امين مصطفى الامين
Supervisor name: زيد محمد مبارك المهداوي | منى حسين جانكير
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: صممت الدراسة الحالية لمعرفة التاثير الوقائي لعصير التفاح الاخضر (Malus domestica) والمستخلص المائي للزنجبيل (Zingiber officinale) من التاثيرات الضارة نتيجة التعرض للاشعة فوق البنفسجية والاشعة السينية في ذكور واناث الجرذان البيض لمدة ثلاثة ايام من خلال قيا | This study is designed to evaluate the protective effect of Green Apple (Malus domestica) juice and the aqueous extract of ginger (Zingiber officinale) against the side effect of Ultraviolet ray (UV) and X - ray in male and female albino rats for three days by examined some physiological and biochemical effects and histotoxicity in liver and kidney tissues. The rats with age (5 - 7) months and weight (250 - 350) g were divided into 16 groups (5 rats for each group) : group (1) control group received drinking tab water and ideal diet, group (2) Ultraviolet ray group only for half hour, group (3) Ultraviolet ray group only for one hour, group(4) UV group for half hour + green apple juice (2 ml/kg), group (5) UV group for half hour + green apple juice (4 ml/kg), group (6) UV group for one hour + green apple juice (2 ml/kg), group (7) UV group for one hour + green apple juice (4 ml/kg), group (8) UV group for half hour +ginger abstract (1000mg/kg), group (9) UV group for half hour +ginger abstract (2000mg/kg), group (10) UV group for one hour +ginger abstract (1000mg/kg), group (11) UV group for one hour +ginger abstract (2000mg/kg), group (12) X - ray group only, group (13) X - ray group+ green apple juice (2 ml/kg), group (14) X - ray group+ green apple juice (4 ml/kg), group (15) X - ray group+ ginger abstract (1000mg/kg), group (16) X - ray group+ ginger abstract (2000mg/kg).The study result showed that : 1. The radiation exposed rats by Ultraviolet ray for half hour and one hour and exposed group to X - ray only caused significant elevation in total count of leukocytes (WBCs), Platelets count, and in the level of blood sugar (glucose), Total Cholesterol, Triglyceride (TG), Low density lipoprotein - cholesterol (LDL - C), Very low density lipoprotein - cholesterol (VLDL - C), Phospholipids, Atherogenic indices levels, Urea, Creatinine, Malondialdehyde, Peroxy nitrate radical, and a significant decrease in Packed cell volume (PCV), Haemoglobin (Hb), count of Erythrocytes (RBCs), High density lipoprotein - cholesterol (HDL - C), total protein, Albumin, Globulin, Uric acid, Glutathione compared with control group.2. when the groups treated by green apple juice (2, 4 ml/kg) and the aqueous extract of ginger (1000, 2000 mg/kg) after that exposing rats to the radiation by Ultraviolet ray for half hour and the exposing groups to Ultraviolet ray for one hour only and the exposing groups to X - ray only showed significant reduction in total count of leukocytes (WBCs), Platelets count, and in the level of blood sugar (glucose), total Cholesterol, triglyceride (TG), Low density lipoprotein - cholesterol (LDL - C), Very low density lipoprotein - cholesterol (VLDL - C), Phospholipids, Atherogenic indices levels, Urea, Creatinine, Malondialdehyde, Peroxy nitrate radical, and a significant increase in Packed cell volume (PCV), Haemoglobin (Hb), count of erythrocytes (RBCs), High density lipoprotein - cholesterol (HDL - C), total protein, Albumin, Globulin, Uric acid, Glutathione compared with groups exposed to Ultraviolet ray only for half hour.3. The Exposing radiation of the rats groups by Ultraviolet ray and X - ray that lead to makes changes in tissues and many abnormalities in animals liver and kidney of these groups, in liver this included necrosis and degeneration in the hepatocytes and fibrosis and observed Atrophy hepatocytes and that lead to loss its normal shape and observed infiltration of Lymphocyte and many ceases of Central vein damage and degeneration of epithelial cells and that caused hemolysis and hemorrhage cases and appear of RBCs in tissue sections. While in Kidney the tissue abnormalities included necrosis and degeneration the epithelial cells of tubules and damage cases of glomerulus, tubeules and blood vesscles and degeneration of epithelial cells and that lead to hemorrhage cases in most area and combined with Infiltration of Lymphocyte.4. when the animals groups treated by green apple juice (2, 4 ml/kg) and the aqueous extract of ginger (1000, 2000 mg/kg) after that exposing rats to the radiation by Ultraviolet ray and X - ray, this abnormalities happened but more less, and observed the normals shaped in some area of tissues sections. The result showed the important and the ability of Green Apple juice (Malus domestica) and the aqueous extract of ginger (Zingiber officinale) in protect against the side effect of Ultraviolet ray and X - ray in male and female albino rats

دراسة عدد من المتغيرات الفسلجية والكيموحيوية المؤثرة في حصى الكلى لدى المرضى في مدينة تكريت وضواحيها == A Study of Number of Physiological And Biochemical Effectual of Renal CALCULI In Patients From Tikrit City And It'S Suburbs

Author name: حلا حميد مجيد جاسم
Supervisor name: وهبي عبد القادر سلمان | نهاد نجرس هلال
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: جرت هذه الدراسة في مستشفى تكريت التعليمي في محافظة صلاح الدين للفترة من تموز (2007) ولغاية كانون الثاني (2008) والتي تهدف لدراسة عدد من المتغيرات الفسلجية والكيموحيوية المؤثرة في حصى الكلى لدى المرضى في مدينة تكريت وضواحيها وتم قياس تركيز هرمون الباراثارم | This Study had been carried in Training Tikirt hospital Salah El - din governorate during the period of July (2007) up to December (2008). This Study aimed to study number of Physiological and Biochemical effectual of renal calcul in Patients from Tikrit City and it's Suburbs and the concentrations of Parathrmone (PTH) and Calcitonin (CT) had been determind and Number of electrolites Calcium (Ca++), Magnesium (Mg++), Phosphorus (P+++) and Uric Acid concentrations and another blood parametrs had been determind too count of White Blood Cell (WBCs) and averge of Erythrocytes Sedimentation Rate (ESR). Sixty patients and Thirty healthy male and female were selected.The total number of patients were (25, 35) femal and male while the total number of healthy were (13, 17). The avarege of their ages were (20 - 80) year.The Medical Staff in Training Tikirt hospital diagnozed the patients and healthyby depending on the Clinical, Lab and Ultra Sonard reports.The results showed that : 1 - There was no significantly increased among patients and healthy at the levels of Hormone (PTH) and (Ca++), While the patients were significantly increased (P<0.05), (P<0.01) comparing with the healthy at the levels of (P+++), (UA), (WBCs) and (ESR). There was significantly decreased (P<0.05), (P<0.01) at the levels of hormone (CT) and (Mg++) in patients comparatively with healthy.2 - The female patients were significantly increased (P<0.05), (P<0.01) at the levels of hormone (PTH), (P+++), (UA), (WBCs) and (ESR) comparing with healthy females while the patients male were significantly increased (P<0.05), (P<0.01) at the levels of hormone (CT), (UA), (WBCs) and (ESR). Healthy males were significantly increased (P<0.01) (Mg++) comparing with patients males and also the healthy females were significantly increased (P<0.05), (P<0.01) at the levels of hormone (CT) and (Mg++) comparing with patient females.3 - The patients were increased in their aged group (20 - 39) year comparing with healthy significantly (P<0.05), (P<0.01) at the levels of (P+++), (UA), (WBCs) and (ESR), while the healthy who were increased in this group significantly (P<0.01) at the level of (Mg++) comparing with patients. According to the group of (40 - 59) year we noticed asignificantly increased (P<0.05) patients comparing with healthy at the levels of (P+++), (UA) and (ESR). The healthy were significantly increased in this group (P<0.05) at the level of (Mg++) comparing with patients. In the group (60 - more) year the patients were significantly increased (P<0.05) comparing with healthy at the levels of hormone (PTH), (P+++), (UA), (WBCs) and (ESR). While the healthy were increased in this group significantly (P<0.05), (P<0.01) at the levels of hormone (CT) and (Mg++) comparing with patients.4 - The patients were significantly increased (P<0.05) in mass group(20 - 25) Kg/m2 comparing with healthy at the levels of (P+++), (UA), (WBCs) and (ESR). While the healthy were increased In this group significantly (P<0.05) at the levels of hormone (CT) and (Mg++) comparing with the patients. In the mass group (31 - more) Kg/m2 significantly increased (P<0.05), (P<0.01) at the levels of hormone (PTH), (P+++), (UA), (WBCs) and (ESR) comparing with healthy. The healthy achieved significantly increased (P<0.05), (P<0.01) at the levels of hormone (CT) and (Mg++).5 - According to the place of living we noticed asignificantly increased (P<0.05), (P<0.01) for patients who were living in cities at the levels of (P+++), (UA), (WBCs) and (ESR) comparing with healthy and they increased at the level of (Mg++). While the patients who were living in district were significantly increased (P<0.05) at the levels of (P+++), (UA), (WBCs) and (ESR) comparing with healthy. The patients who were living in village were significantly increased (P<0.05) at the levels of (P+++), (UA), (WBCs) and (ESR) comparing with healthy while the healthy achieved significantly increased (P<0.05) at the levels of hormone (CT) and (Mg++) comparing with patients.

تحديد تجمعات Giardia lamblia ومعقد Entamoeba histolytica/Entamoeba dispar بالتشخيص الجزيئي (PCR) للمصابين في تكريت == Determination of Giardia Lamblia Assemblages And Entamoeba Histolytica/Entamoeba Dispar Complex By Molecular Diagnosis (Pcr) In Patient In Tikrit

Author name: انتصار غانم عبد الوهاب الصميدعي
Supervisor name: شهاب احمد محمد الجبوري | عقيل حسين العاصي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة للمدة من كانون الثاني 2011 ولغاية كانون الاول 2011 للمرضى الراقدين والمراجعين لمستشفى تكريت التعليمي وبعض المختبرات الخاصة في مركز المدينة وضواحيها والذين يعانون اعراض مرضية مختلفه ولفئات عمرية مختلفة ترواحت بين اقل من سنة الى 61 سنة، ح | This study was conducted for the period started from January 2011 until December 2011 on inpatients and outpatients of Tikrit educational hospital and some private laboratories at Tikrit city and surrounded suburbs. Symptoms of different diseases among various age groups varies between less than one year to 61 years. The tests used the (ELISA & PCR) and the analysis of the PCR - RFLP to discriminate the infection by assemblage of Giardia lamblia and differentiate between pathogenic amoeba Entamoeba histolytica and nonpathogenic Entamoeba dispar compared with microscopical examination. The result of present study showed that (75) person who represent (37.5%) were infected by Giardia lamblia and (75) person who represent (37.5%) were infected by E.histolytica/E.dispare complex, out of (200) stool samples which have been tested by microscopical examination. Epidemiological study showed no significant difference among the sex for the two species parasites whereas a high percentage for infection recorded in group at age (1? - 10) year for Giardia lamblia and E.histolytica / E.dispar complex reach to (60%, 36%) respectively. Regarding the place of living Giardia lamblia in rural area showed infection rate of (19.5 %), while infection rate by E.histolytica/E.dispar complex reaches (22.5%) in urban area. Statistical analysis shows a high difference of significance according the source of water supply. Tap water showed high incidence for initiating infection which reaches to (93.3%) by G.lamblia and (90%) for E.histolytica/E.dispar complex. For the infection rates and their relationship to the educational level, the results show that the highest rates of infection recorded in non - educated (52%) and (73.3%) of Giardia lamblia and E.histolytica / E.dispar complex, respectively. Depending on the sample texture, the result show that oily diarrhea is more correlate with infection reaches to (33.3%) of Giardia lamblia and the watery diarrhea form 40% by E.histolytica / E.dispar complex, while simultaneous occurrence of both symptom (colic and loss of appetite) are the most symptom synchronized with infection records (44%) for Giardia lamblia and (36%) for E.histolytica / E.dispar complex. The percentage of infection by Giardia Lamblia in this study depends on the test results of the ELISA was (20%), while the percentage of infection E.histolytica/E.dispar complex was (6.7%). Testing has shown sensitivity compared to microscopic examination reaches to (22.6%) and (8%) for both parasite respectively, while the test showed a high specificity which reaches the rate (93.3%) and (100%) for both parasites respectively. Result of PCR showed a percentage of infection by E.dispar about (32%) (64 sample) from the total samples, whereas it does register any cases infection by E.histolytica. By counting the results of the PCR for the three genes (ssu rRNA + TPIA + GDH) they show a total infection by Giardia lamblia which was (10.5%) (21 samples). This study recorded percentage of infection Giardia lamblia depending on the result of the three Genes separately amounted to (4%), (5%), (3%) for (ssu rRNA, TPIA, GDH) respectively. According to the result of the analysis PCR - RFLP for GDH gene by using the enzyme NIaIV, it did not record any infection by Giardia lamblia (assemblage A), whereas (6 samples) recorded (100) by Giardia lamblia (assemblage B), by using the enzyme Rsal to determine the secondary assemblage shows that the total (6 samples) infected there are 5 samples carrying infection by two type of secondary assemblage returning to the assemblage B (assemblage BIII +BIV) and only one sample infected with (assemblage BIV). To determine the assemblage and secondary assemblage for the parasite result compared with amplification of TPIA gene with lysis by enzyme for the resulting amplification for GDH gene, resulting a ratio of (14.3%) for a sample infection by both assemblage (A+B) and secondary assemblage (BIII+BIV) together, and a ratio of (57.14%) infection by assemblage A only and (21.4%) infection by secondary assemblage (BIII+BIV) together and (7.14%) infection by BIV only.

دراسة التاثير المثبط لبعض انواع بكتريا حامض اللاكتيك ضد بعض انواع الفطريات الممرضة والمنتجة للسموم == Study The Inhibition Effect of Some Species of Lactic Acid Bacteria Against Some Pathogenic And Toxins Producing Fungi Types

Author name: لؤي برهان مصطفى محمد
Supervisor name: كركز محمد ثلج الجبوري
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت الدراسة في مختبرات قسم علوم الحياة في كلية التربية بهدف عزل وتشخيص بعض انواع بكتريا حامض اللاكتيك ومحاولة اختبار قابلية خلاياها او النواتج الايضية منها وكذلك دراسة تاثير المستويات المختلفة من درجة الحرارة والاس الهيدروجيني في القابليـة التثبيطية لب | This work aimed to investigate the effects of some lactic acid bacteria (LAB) species (cells or their metabolic products) at different temperatures and pH levels on some soil pathogenic or toxin produced fungi species. Six LAB isolates and the identification was depended on the morphological and cultural characterized and biochemical testes, the isolates were determined as Lactobacillus delubricii subsp. delubricii, Lactobacillus delubricii subsp. bulgaricus, Lactobcillus casei, Lactobacillus acidophilus, Leuconostoc mesentroides and Streptococcus thermophilus, and four fungal species Microsporum canis, Trichophyton mentagrophytes, Aspergillus fumigatus and Rhizopus stolinfer were identified. In this study the highest inhibition effect against the above mentioned fungi was shown by L. acidophilus as a significant (P< 0.05) efficacy where the diameter of inhibition zone in case of M. canis, T. mentagrophytes, A. fumigtus and R. stolonifer was 26, 32, 30 and 28 mm respectively. Less inhibition activity was exhibited by Leu. mesentroides and Str. thermophilus while the lowest inhibition was shown by L. delubricii. Results showed also that the temperature optimal for the growth of bacterial species (except L. casei and Str. thermophilus) tested and their inhibition activity was 30 °C. However, temperature of 35 °C was the optimal for the highest inhibitory effect of both L. casei and Str. thermophilus. At 25 °C of studied bacterial species showed decreased inhibitory effect against the tested fungi. Concerning the pH, the present study showed that the highest inhibitory of all tested bacteria species (except L. acidophilus) was at pH 6.5, L. acidophilus showed such activity at pH 5.5. This study revealed that the inhibitory effect of metabolites produced mixture of the tested bacterial species was directly proportional with its concentrate. Where this mixture was used at media concentration of 10, 20 and 40 mg/ml cultural. However, metabolic products from L. acidophilus, Leu. mesentroides and Str. thermophilus was the most effective in inhibition activity compared with the rest of the bacteria species used particularly at 40 mg/ml cultural media

دراسة بكتريولوجية لبيئة بعض كهوف شمال العراق والتحري عن الدور الجرثومي فيها == A Bacteriological Study On Some North Iraqi Caves Environment And Detection On The Bacterial Role In It

Author name: امنة غانم عمر العاني
Supervisor name: جهاد ذياب محل الجنابي | اميرة محمود محمد الراوي
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة اجراء عدد من الفحوصات البيئية والبكتريولوجية على عينات الصخور ومياه النضح البالغ عددها 100 عينة والماخوذة من بعض كهوف المنطقة الشمالية في العراق التي شملت بهيري, شرانش, خنس, بجيل وانشكي للفترة ما بين تشرين الثاني - 2010 لغاية نيسان - 2011.ت | This study was included some of ecological and bacteriological tests on rocks and dripping water samples which were included 100 samples taken from some of north region caves in Iraq including Beherii, Sharanish, Khanas, Bejeel and Inishky from the period between November - 2010 to April - 2011.The results of ecological tests which represented with cave environment temperature were ranged from (13 - 24)?C and dripping water temperature were ranged from (11 - 20)?C while the results of pH values for the same sample shows simple variation between (7 - 8) for dripping water and (6 - 9) for the rocks samples. The results of geological screening and mineralization by using X - Ray Fluorescence and X - Ray - Diffraction showed that Beherii cave was mainly composed of manganese with 102 ppm comparing with other caves. While the concentration of iron and sulfur oxides were very low for all caves. The rocks of Beherii and Khanas caves were mainly composed of Dolomite (CaMg (CO3)2), Sharanish cave was composed of Calcite (CaCO3) and Dolomite with the closed ratio, while the rocks of Bejeel and Inishky were composed mainly of Calcite. The isolated bacteria in this study were identified according to cultural and morphological characteristics and biochemical tests, while the bacillus species were identified by using Microgen Bacillus Id System. Twenty two bacterial isolates were identified from total samples. There were nine (9)identified isolates from Beherii cave with 40.9% including Arthrobacter spp., Lysobacter spp., Caulobacter spp., Bacillus freudenreichii and Bacillus subtilis in with 4.5% for each isolate, while there was 18.1% of Methanogenic bacteria with its four genuses including Methanobacterium spp., Methanobrevibacter spp. Methanospirillium spp. and Methanosata spp. In Sharanish cave there was one isolate of sulfur oxidizing bacteria with 4.5 % from the total isolates. Three isolates were identified from Khanas cave included Pseudomonas spp., Xanthobacter spp., Clostridium spp. with 4.5% for each isolate. In addition, it was obtained from a cave Bejeel on six isolates included Bacillus lichiniformis, Bacillus lentus and 2 isolates of Bacillus cereus group, Streptomyces spp. and Gallionella spp. with 4.5% for each isolate and Merismopedia spp., Myxococcus Xanthus, Clostridium spp. were Isolated from Inishky cave with 4.5%for each isolate. The relation between some of bacterial species were studied by detection of the lytic ability of Lysobacter spp. and Myxococcus xanthus for Arthrobacter spp.The microbial role in caves were studied by detection of the ability of some isolated bacteria in Calcium Carbonate (calcite) formation and estimatation of calcite production, Xanthobacter spp. was the highest productive bacteria which gives0.2073 gram during 14 days comparing with other bacterial species.In addition, the effect of temperature and pH on calcite formation by Bacillus freudenreichii were studied, the results showed variation on calcite shape in 20?C and 30?C while the highest value of calcite production in pH.8 was 0.1336 gram comparing with pH.9 with 0.0190 calcite gram. The study also detected the ability of some isolated bacteria to oxidize of iron, manganese and sulfur, where Streptomyces spp. Gallionella spp. and one isolate of bacillus cereus group have shown the ability of oxidize iron on leptothrix medium while Bacillus freudenreichii, Bacillus lichinformis, Bacillus lentus, one isolate of Bacillus cereus group and Streptomyces spp. have shown the ability to the oxidize of manganese on Krumbein and Altmann Agar Medium. In addition, sulfur oxidizing bacteria has shown ability to oxidize sulfur on M.S. Agar and produce sulfuric acid as a product of the oxidation process.

دراسة تاثير مياه مجاري سامراء على نوعية المياه في نهر دجلة == A Study On The Effect of Samarra Sewage Water On The Quality of Water In Tigris River

Author name: افراح طعمة خلف مطر البدري
Supervisor name: حميد سلمان خميس المهداوي | ياسين حسين عويد
General topic: Biology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت هذه الدراسة اختيار خمس محطات اربعة منها على نهر دجلة المار بمدينة سامراء بعد جسر سامراء والمحطة الخامسة والتي تمثل رقم 2 على مياه مجاري سامراء قبل ان تصب في مياه نهر دجلة.وقد تم اخذ العينات للمدة من شهر تشرين الاول 2010 حتى نهاية شهر ايلول 2011 وقد | The study included chosen five stations for water sampleing, four of them at Tigris river while the fifth station was chosen at samara sewage before meeting with Tigris river.Samples were collected monthly started from October 2010 until the end of September 2011. The study included the study of some physical, chemical and biological characteristics for river water and sewage and noticed the effects of sewage water on quality of these water in Tigris River, these characteristics clued in air , water temperature, electrical conductivity, pH, total dissolved solid, Total Suspseneded solid, dissolved oxygen(Do), biological oxygen demand (BOD), total hardness, calcium and magnesium hardness, chloride, alkalinity, nutrients concerteration, ( phosphate, nitrate) in addition the total number of bacteria and coliform bacteria.Water Temperature ranged through out the study ranged between (5° - 35°), while air temperature ranged between(5° - 38°) , Electrical Conductivity in the river ranged between (280 - 1480) mc/cm, where in sewage the electrical conductivity ranged between(1200 - 2180) mc/cm, while pH value tened to be slightly alkaline, it show very close value where ranged between(5.3 - 8.8). It was noticed that the pH value tened to be slightly acidic from January to August 2011. The results also show that the water were in good airation where the value of dissolved oxygen ranged between (3 - 10) mg/L in station one and stations five while the value of dissolved oxygen in stations two and three range between (2 - 6.5) mg/L.The result also showed that the concentration of total suspended solids ranged between(20 - 798) mg/L. where it has high value in April in all stations, this propably due to increase of discharge of the river which accompanied by suspended solid, while the dissolved solids in all station range between (250 - 1470) mg/L.The highest value recorded were, 1470 mg/ L on May 2011in station two.The value of total hardness for Tigris river ranged between hard to very hard(125 - 360) mgCaCO3/L in station one, four and five, whereas its value in stations two and three ranged between (200 - 925) mg/L. Calcium ion which is the cause of calcium hardness was higher than magnesium cencenrteration which is responsible of magnesium hardness, where calcium hardness ranged between (75 - 587.5) mg/ l and magnesium hardness ranged between (5 - 500) mg/l. chloride concentration ranged between (8 - 127.5) mg/l in all station, chloride cencetration in stations one, two, and five were in the allowable wnorld limit(8.5 - 68) mg/l. Alkalinity results showed that it related to carbonate and bicarbonate, and its ranged between (110 - 900) mg/l.While phosphate value ranged between(0.0106 - 1.999) µg atom - PO4/L, the lowest values were recorded in Tigris river, While the high value recorded in sewage station two, while nitrate value were fluctuated in all stations but the high value recorded in the second station is ranged between (0.0103 - 1.140) µg atom NO3/l. Result of microbiological study shown that there is high number of bacteria the average of total count of bacteria were(39.7×105، 17.31×105، 267× 105، 3.17 ×105، 0.0531×105) cell/ ml for all stations respectively, so it has been suggested that the water in Tigris river considered as polluted to very polluted while the resulty of Coliform bacteria were very high and the average number for all stations respectively as follows (209 × 105, 9809×105 , 25× 105, 1×105, 0.004×105 ( cell /ml According to these result the water is not suitable for direct drinking.Statistical analysis for most factors studied show significant differences between station one and station two and between station one and station three as well in stations four and five and there is no significant differences between station two and station three.

تاثير كبريتات الخارصين في احداث افات نسجية مرضية وتغيرات وزنية في بعض اعضاء الفئران البيض Mus musculuc الحوامل وتشوهات خلقية ظاهرية لاجنتها == Effect of Zinc Sulfate In Histopathological Lesions Production And Weight Changes In Pregnant White Mice Mus Musculus And Morphological Congenital Malformations of It'S Embryos

Author name: محمود نوفل مصطفى حمد الصالح
Supervisor name: عزيز خالد حميد | سهام توفيق امين
General topic: Biology
Specific topic: Zoology - Histology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: درس قابلية الجرعات العالية من كبريتات الخارصين في احداث تغيرات مرضية نسجية في اناث الفار الابيض السويسري الحوامل وتشوهات خلقية في اجنتها، حيث جرعت الحوامل بمحلول كبريتات الخارصين المائية داخل المرئ، حيث قسمت الاناث الى خمس مجاميع الاولى : جرعت بماء مقطر

دراسة تاثير جنس الجنين وبعض المتغيرات الفسلجية في تراكيز بعض الهرمونات والمتغيرات الاخرى في دم الحوامل لمدينة تكريت وضواحيها == Study The Effect of Fetus Gender And Some Physiological Variables On The Concentrations of Certain Hormones And Other Variables On Pregnant Blood In Tikrit City And Its Suburbs

Author name: مروه عصام سليمان
Supervisor name: وهبي عبد القادر سلمان
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: جرت هذه الدراسة في مختبرات مستشفى تكريت التعليمي ومراكز الرعاية الصحية ومختبر علوم الحياة في كلية التربية للبنات جامعة تكريت وضواحيها للفترة من تشرين الاول2011 ولغاية اذار 2012 لمعرفة تاثير مراحل الحمل وعوامل جنس الجنين وكتلة الجسم الحي وعمر الام والسكن ل | This study was conducted in the laboratories of Tikrit Teaching Hospital, health - care centers, and the laboratory of Biology in college girls education in Tikrit city and its suburbs from the period of October 2011 until March 2012. The study was performed to see the impact of the pregnancy stages; sex factors of the fetus; body mass index; the mother's age; and residence for pregnant women, in the concentration of : certain blood components (Hb, PCV, WBC); blood sugar; cholesterol; estrogen, progesterone and testosterone hormones; and (TSH, T3, and T4 hormones. The study included (90) women at different stages of pregnancy, and the number of pregnant women at every stage was (30), ranged their ages from (15 - 40) years. A sample of (10) ml blood was taken from the surveyed women. The serum was separated by the common methods of separation, and conducted thereon desired examinations, The outcomes were as follows : - 1. Superiority of the first and second stages of pregnancy significantly (P<0.05) in the concentration of (Hb, PCV), compared with the third stage. 2. Increasing the total count of white blood cells and the concentration of blood sugar and cholesterol significantly with the progress of pregnancy stages. 3. Increasing progesterone hormone concentration significantly with the progress of pregnancy stages, while the concentration of estrogen hormone decreased in the middle stage of pregnancy, and increased significantly in the first and third stages, whereas there were no significant differences in the concentrations of testosterone hormone during the different stages of pregnancy. 4. The concentration of TSH and T3 hormones were decreased significantly with the progress of pregnancy stages, while the concentration of T4 hormone was increased in the first and last stages of pregnancy.5. Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of Hb and PCV ratios during the first and second stages of pregnancy, while the opposite happened in the third stage.6. Superiority of male pregnancy significantly compared with the female pregnancy in the total count of white blood cells during the first and third stages of pregnancy.7. Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of blood sugar, while the female pregnancy surpassed significantly in the concentrations of cholesterol compared with the male pregnancy during all the pregnancy stages. 8. Superiority of female pregnancy significantly compared with the male pregnancy in the concentration of estrogen hormone in first stage, while the opposite happened in the third stage, while the male pregnancy surpassed significantly compared with the female pregnancy in the concentrations of testosterone hormone during second and third stages, as well as superiority of female pregnancy significantly compared with the male pregnancy in the concentration of progesterone hormone during the first and third stages of pregnancy, while the opposite happened in the second stage.9. Superiority of female pregnancy significantly compared with the male pregnancy in the concentrations of TSH hormone during the first stage of pregnancy, while the opposite happened in the third stage. while Superiority of male pregnancy significantly compared with the female pregnancy in the concentrations of T4 hormones during the first and second stage of pregnancy but the concentration of T3 superiority all pregnancy stages.10. A significant decrease in the values of Hb and PCV with increasing the body mass index during the second stage of pregnancy while the opposite happened in third stage of pregnancy.11. Increasing the total count of white blood cells significantly with increasing the body mass index in the first stage of pregnancy, while the opposite happened in the last stage of pregnancy. Increasing the concentration of blood sugar with increasing body mass index in first and second stages of pregnancy but concentration cholesterol decreasing significantly with increasing the body mass index.12. Decrease the concentrations of estrogen hormone significantly during first and second stages of pregnancy with increasing the body mass index for pregnant women, while a significant increase appeared in the concentration of progesterone hormone with increasing the body mass index during the second stage of pregnancy, and the opposite happened during the first and third stages. Testosterone hormone increased significantly with increasing the body mass index for pregnant women during first and second stages of pregnancy 13. A significant increase appeared in the concentrations of TSH hormone at pregnant women with decreasing the body mass index. while a significant increase for the concentration of T3 in first stage and T4 hormone increase at pregnant women during all stages of pregnancy with increasing the values of body mass index.14. No significant differences in values of Hb and PCV during the first and second stages of pregnancy for all ages, but the small ages surpassed significantly compared with the old ages during the third stage of pregnancy. while The total count of white blood cells decreased significantly with the progress of pregnant women's age during all pregnancy stages.15. The small age groups for pregnant women increased significantly in the concentration of blood sugar during the first stage of pregnancy, while they decreased significantly during the second stage of pregnancy.while Age did not affect significantly in the concentrations of cholesterol during the first stage of pregnancy, while the old age groups decreased significantly compared with the first and second age groups) 32 - 24), (23 - 15) during the second and third stages of pregnancy.16. The concentration of Estrogen hormone increased significantly with the age progress during the first and third stages of pregnancy compared with the second stage, while the concentrations of progesterone hormone decreased significantly at old ages during the first and third stages of pregnancy.The concentration of testosterone hormone was not affected significantly during the last stage of pregnancy for all ages, but it decreased significantly during the second stage of pregnancy compared with the first stage of pregnancy for all ages.17. A significant increase showed in for the concentration of TSH in first and the last stages while decreased significantly the concentration T3 and T4 hormones with the age - progress at all stages of age.18. No noteworthy impact for residence of pregnant women in all stages of age, cause its impact is very little on all the qualities covered in the present study.

دراسة المشعرة المهبلية Trichomonas vaginalis والجراثيم المرافقة لها في نساء مدينة تكريت == Study of Trichomonas Vaginalis And Bacteria Associated With It In Tikrit City'S Women

Author name: ميلاد عدنان مزهر
Supervisor name: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تناولت الدراسة الحالية عزل طفيلي المشعرة المهبلية T. vaginalis والتعرف على انواع البكتريا المرافقة له من النساء اللواتي يعانين من التهاب المهبل Vaginatis المراجعات لمستشفى تكريت التعليمي قسم النسائية والتوليد للمدة مابين اب 2007 ولغاية نهاية شباط 2008،بلغ | The present study included the isolation of T. vaginalis and conducted to determine bacteria associated with this parasite in women attending gynecology and delivary department in Tikrit teaching hospital suffering from vaginitis from august 2007 till the end of February 2008. One hundred ninety three samples were collected, their ages ranged between (14 - 57) years.The rate of infection with this parasite was 9.3%. it was not associated with bacterial in a proportion 33.3%, where as 66.7% was associated with bacterial infection.Bacterial vaginitis represented 80.4%, S. aureus was the most commonly isolated associated with T. vaginalis. It was with a proportion 41.8% and was the most common bacteria represented 21.3% among other bacterial species causing vagintis. The sensitivity of diagnostic tests were variable since the direct examination was the highly sensituse test (100%) in comparison with staining using giemsa and gram stains, their sensitivity were 19.6% and 0.0% respectively. The most common clinical symptoms related to trichomoniasis infections were discharges with burning and itching which represented 44.8% and white discharge was 64.7% which represent discharge according to color.. According to epidemiology of this parasite, the highest percentage of infection was 14.6% in urban women with secondary educational level and with high socioeconomic level while represent 22.7% and 15.8% respectively and the highest rate of infection (12.9%) as recorded in ages group (24 - 33) years. In married women the proportion of infection was 9.4% while in women whom were not child bearing it was 15.9%. Also a highest infection rate 16.6% was recorded in women who using antifertility tablets. The present study also determined the relationship between the pH of vagina and types of infection since high infection rate was with parasite 91.7% and 97.4% of bacterial vaginatis at pH ? 4.5. The present study showed a highly significant elevation in E2 hormone valvo in pregnant and nonpregnant women infected with trichomoniasis while this hormone was decreased in sterile women infected with trichomoniasis and in pregnant and nonprengnant and sterile women infected with bacteria vaginatis.

دراسة معالجة مياه الفضلات لمصافي بيجي وامكانية استخدامها كمياه لري بعض الخضر == Study of Wastewater Treatment For Baiji Refinery And The Possibility of Using It As Water Some Verdures

Author name: مريم عدنان ابراهيم
Supervisor name: خليل ابراهيم بندر النعيمي
General topic: Biology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت الدراسة في قسم علوم الحياة - كلية التربية - جامعة تكريت من شهر تشرين الثاني 2010 الى شهر تموز 2011 ? وكان هدف الدراسة هو معرفة بعض الصفات الفيزياوية والكيمياوية للمياه المطروحة من شركة مصافي الشمال/بيجي وامكانية استخدامها لري بعض المحاصيل الزراعية ب | This study was conducted at the Department of Biology in the College of Education - University of Tikrit. The study aims at estimating some physical and chemical characteristics of water raised from the North Refineries Company / Baiji and can be used to irrigate some crops after treatment with a number of physical methods. It also aimed at estimating some heavy elements and their impact on the growth of plants that have been studied (tomato, eggplant, sweet pepper, hot pepper). The region from which samples have been brought of the area was Albu Juwaree village that is located in the city of Baiji and adjacent to the company. Besides, it occurs near wide agricultural areas. After estimating some physical and chemical characteristics of water, soil, and the proportion of oil waste, qualities of plants were studied as well as the assessment of (carbohydrates, proteins) and some of the heavy elements and their effects on the growth of those plants.The results showed that the water of industrial waste led to a reduction in the proportion and natural growth of plants. The most affected seeds were the seeds of sweet pepper, where the total percentage of germination was(0%) when infected %)100) and the least affected seeds were the seeds of tomato, where the total percentage of germination seeds was (80%) when water is contaminated (100%). The treatment by filtering has achieved the best germination of seeds of tomato and sweet pepper, where the ratio was (100%). It turned out that contaminated water has prevented the natural growth of those plants in respect to the number and the size of leaves, the length of roots and the weight of fresh and dry leaves. The methods of physical treatment showed that lightening (25%, 50%, 75%) made to the contaminated water led to the removal of the significant effects, especially when lightening was (75%).The results indicate that there is no significant differences in the lengths of the roots of tomatoes and eggplants, except for the root of hot and sweet pepper.While the heavy elements, which was part of the study in this research, beginning with cadmium was at its highest value at the treatment by filtering and less value in (100%). Through the study it was found that the percentage of lead in all studied plants were (0.00) ppm. The Results showed that the contaminated water led to the reduction of the proportion of carbohydrates with the increase of pollution(%100), where the least proportion of carbohydrates was at the treatment by filtering. The results also showed a decrease in protein concentration with the least proportion at lightening and less value in. (25%)The results showed that the water content of contaminated industrial heavy metals studied (cadmium - lead - copper - zinc - nickel) has decreased at all treatment when treated by filtering, as well as for the remaining elements, except lead, which was (0.00) in all plants and all lightening ratios.The results of this study show that treatment of contaminated water with industrial oil waste by physical methods led to minimizing the negative impacts of such water in reducing the percentage of germination and the reduction below the natural growth compared with non - treatment water and showed that the best way of treatment for the best growth of plants were lightening with (75%) and treatment by filtering. The best response to the treatment was tomato and the most resistant to pollution were (tomato and eggplant) and the most affected by contamination and least resistant was sweet pepper.

عزل وتشخيص بكتريا Streptomyces المنتجة للمضادات الميكروبية من ترب بعض اقضية محافظة صلاح الدين == Isolation And Identification of Antimicrobial Producing “Streptomyces” From Soils of Some Districts In Salahaddin Province

Author name: هيام عزيز عباس البدري
Supervisor name: عبد الكريم فتاح عمر
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة عزل وتشخيص انواع تابعة للجنس Streptomyces، اذ جمعت 50 عينة من ترب اقضية تابعة لمحافظة صلاح الدين. شخصت?11 نوع من بكتريا Streptomyces باستخدام الاختبارات الشكلية والمزرعية والكيموحيوية حيث ظهر كل من الانواع (Streptomyces antibioticus, Strep | The Study involved ; isolation and characterization of species related to Streptomyces. Fifty samples of soil has been taken from different districts of Salah aldden province. The isolates were identified by the morphological, biochemical tests and classified to nine species, The following species were found : (Streptomyces antibioticus, Streptomyces coelicolor , Streptomyces distalicus and Streptomyces fluvissimus ) in Tikrit district, ( Streptomyces cyaneus ) in Ballad and beiji districs, ( Streptomyces exofolicus) in Ballad and Aldor districts (Streptomyces lavendulea ) in Samarra and Aldor districts , (Streptomyces purpureus ) in Samarra and Beiji districts and (Streptomyces violaceus ) in Samarra and Tikrit districts. The sensitivity of these bacterial isolates were tested toward the following : (Azithromycin, Cefotaxime, Cephatexin, Doxycycline, Erythromycin , Gentamicin, Neomycin, Penicillin, Tobramycin and Vancomycin).All species showed high resistance to cefotaxime. But all species were sensitive to neomycin and doxycycline. The isolated species of Streptomyces were tested for their ability of antibiotic production. It was found that (Klebsiella) was sensitive to all the species except species Streptomyces coelicolor. Streptomyces coelicolor showed an effect against Proteus. While Staphylococcus aureus was sensitive to the following species (Streptomyces antibioticus, Streptomyces cyaneus and Streptomyces purpureus). The Scanning Electron Microscopy was used to study the surface of spores. The species of (Streptomyces antibioticus) was found to has warty surface while spores of ( Streptomyces coelicolor) has smooth surface.

دراسة بعض المتغيرات الفسلجية وتركيز البروستاكلاندينات عند مرضى الربو

Author name: سيماء عبد الرحمن شعبان
Supervisor name: زيد محمد مبارك المهداوي | صالح محمد رحيم العبيدي
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت هذه الدراسة لمعرفة بعض المتغيرات الدموية والفسلجية عند مرضى الربو، وقد شملت 46 مريضا بالربو من كلا الجنسين (31 مريضا من الاناث و15 مريضا من الذكور) تراوحت اعمارهم بين 21 - 75 سنة من ضمن الحالات الواردة على مستشفى تكريت التعليمي والمركز الاستشاري لام

معالجة مطروحات مياه فضلات الصرف الصحي لمدينة تكريت باستخدام تقنية المجففات الحرارية الدواره واعادة استخدامها كاسمدة عضوية == Treatment of Domestic Wastewater Effluent of Tikrit City Using Thermal Rotating Dryer Technique And Reuse As Organic Fertilizers

Author name: هالة ارشد علي حسين
Supervisor name: جهاد ذياب محل الجنابي
General topic: Biology
Degree: Doctorate
Language: Arabic
University location: Salahaddin
First pages:
Abstract: اجريت الدراسة في مختبرات كليتي التربية والعلوم - جامعة تكريت للفترة من نيسان 2010 لغاية شباط 2011 تتضمن الدراسة معالجة الحماة الناتجة عن حوض التجميع الابتدائي باستخدام تقنية المجففات الحرارية الدوارة للعمل على ازاحة ماء الحماة والتي تمثل اصعب العمليات الت | This study was conducted at a period from April 2010 to February 2011, to treat the sludge produced from collection tank at tikrit domestic wastewater plant, using thermal rotating dryer technique(T.R.D) or dewatering the sludge.This process represents one of the difficult applied process for the sludge treatment. This technique considered a modern technique in this field.Physical, chemical and biological characteristics of the manufactured sludge were determined and compared with organic fertilizers (I.O.F) imported from republic of Estonia (Available in local markets).T.R.D technique induced dewatering of the treated sludge at ratio48.5 - 61.5% and producing highly sterilized organic fertilizers which was free of pathogenic organisms(bacteria, worms and eggs) with high chemical characteristics comparing with those imported from Estonia. Manufactured organic fertilizers M.O.Fs have allow acidity pH ranged between 7.5 - 7.7 ,. electrical conductivity at range 1630 - 1873, organic matter at ratio 4.56 - 5.35%, with a moderate concentrations of Pb, Cd, Zn.The concentration of N and K was 1.8, 5.96% respectively while the concentration of phosphorus are 350 ppm. Three levels of M.O.Fs were used for corn plants(5, 10 and 20ton/acres). Results showed that all these levels significantly induced increasing for some physiological and morphological characteristics of corn plants compared with those treated by I.O.Fs or control plants. The vegetative dry weight of corn plants fertilized by M.O.Fs were 23.95gm/plant compared with 16.3 and 5.3 gm/plant for plants fertilized by I.O.Fs and control respectively. (M.O.Fs) induced significant increase in nitrogen concentration compared with plants fertilized by I.O.F and control while the concentrations of Pb, Cd, Zn were not affected by using of M.O.Fs on the other hand Pseudomonas aeruginosa and Escherichia coli were contributed in reducing and removing some of pollutants from the sludge at three incubation periods (30, 60 and 90 day). These two species caused significant decrease in pH and EC of the sludge, P. aeruginosas showed the highest removing ratio of nitrogen (93.1%). The cooperation of the two species caused high removing ratio for the Total organic carbon(87.72%) at 60 days and organic materials (90.68%) at 90 days of incubation also showed high removing ratio for phosphors 100%) at 90 days of in cubation.) These two species also caused high removing ratio for pb, Cd and Zn at30 days and P. aeruginosa was more effective in removing Pb, Cd and Zn from the sludge then E. coli.

دراسة بكتريولوجية كيمياوية وفيزياوية لمياه الشرب المعدنية المتداولة في الاسواق المحلية لمدينة تكريت == Bacteriological Chemical And Physical Study For Drinking Mineral Bottled Water Circulating In Local Market In Tikrit City

Author name: اسراء حمود عبد الدوري
Supervisor name: حميد سلمان خميس المهداوي | ياسين حسين عويد
General topic: Biology
Specific topic: Environment
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تضمنت الدراسة الحالية جمع عينات شهرية من اصناف المياه المعدنية المعباة المتداولة في الاسواق المحلية في مدينة تكريت ابتداء من شهر تشرين الاول عام 2010 م ولغاية شهر حزيران عام 2011 م وتم تقييم جودة عشرة اصناف من المياه المعباة بالاضافة الى ماء الصنبور ومقار | The current study included collected monthly samples of mineral water circulating in the local markets in the city of Tikrit from the month of October in 2010 until the month of June in 2011. It was evaluated the quality of ten varieties of bottled water and comparing the results to the standard specifications for Iraqi drinking water and international standards for the World Health Organization.Evaluation included a study of physical, chemical and microbiological factors : turbidity, total dissolved solid, total suspended solid, electrical conductivity, pH, dissolved oxygen, biological oxygen demand, total alkalinity, acidity, total hardness, calcium, sodium, potassium, salinity, chloride, phosphate, nitrate, sulphate, heavy metals, total number of bacteria and total coliforms.The level of quality parameters of these classes were in compliance with the different standards except for pH in one class Pearl and phosphate in one class Rovian.Comparing bottled water to tap water found that the concentrations of turbidity, total dissolved solid, total suspended solid, electrical conductivity, salinity, dissolved oxygen, total hardness, acidity, nitrate, iron, manganese and total number of bacteria in tap water higher than the concentrations in bottled water but didn’t exceed the upper limit that allowed by the local and international specifications for drinking water.Measurements also revealed that the values of most of the criteria listed on the packaging do not reflect the actual content of these water bottles with the exception of one class Kameran most of the packaging in this class has matched the actual content of the water container.The results also showed the absence of coliform bacteria in any of the varieties of bottled water and tap water as well as the significance of the absence of bacterial contamination in it.

تاثير الحمل في بعض وظائف الكبد وعدد من المتغيرات الدموية والكيموحيوية == The Effect of Pregnancy On Some Liver Functions And Blood And Biochemical Changes For The Pregnant Women In Al - Alam

Author name: رؤى حسين علوان الجبوري
Supervisor name: صالح محمد رحيم العبيدي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تناولت هذه الدراسة تاثير الحمل في وظائف الكبد وعدد من صفات الدم الكيموحيوية حيث تم جمع عينات دم من نساء حوامل خلال مراحل الحمل الثلاث ومن نساء متزوجات غير حوامل كمجموعة سيطرة، وجرت هذه الدراسة في مركز العلم الصحي للفترة من اب 2008 الى ك 1 2009 وبلغ مجموع | This study took the effects of pregnancy on liver functions and number of biochemical blood characteristics, in which blood samples were taken from pregnant women during the three trimesters of pregnancy and from non - pregnant married women as a control group, and this study was done in The Alam health centre from August 2008 to December 2009, and the number of pregnant women were (120) women while the non - pregnant married women were (40) women, there ages were between 15 - 40 years old. The results of this study showed increase in Body Mass Index for pregnant women with progress of pregnancy compared with the non - pregnant married women givingP value (P<0.01) and there was appearance of specific elevation in Fibrinogen concentration in blood and Serum Albumin and Cholesterol concentration and Triglyceride in pregnant women compared with the non - pregnant married women giving P value (P<0.01). Also the results showed specific decrease in Bilirubin during the three trimesters of pregnancy compared with the non - pregnant married women especially in the third trimester giving P value (P<0.01). Also the results showed specific elevation in liver enzymes represented by Alkaline phosphatase and Aspartate transaminase enzyme and Alanine transaminase enzyme especially in the last two trimesters of pregnancy in comparision with the non - pregnant married women giving P value (P<0.01), but for Malondialdehyde, the results showed specific elevation during the three trimesters of pregnancy compared with the non - pregnant married women in contrast to the Glutathione antioxidants that showed specific decrease during the three trimesters of pregnancy in comparasion with the non - pregnant married women giving P value (P<0.01). And the study showed also the effects of pregnancy on the blood elements, in which there was obviouse decrease in number of Platelets during the three trimesters of pregnancy compared with the non - pregnant married women giving P value (P<0.01). And the Heamoglubin concentration, Red Blood Cell number and the mean corpuscle Volume Suffer Specific decrease during the three trimesters of pregnancy compared with the non - pregnant married women giving P value (P<0.01).

عزل وتشخيص بعض انواع بكتريا السالمونيلا من بعض الاغذية المحلية في مدينة تكريت == Isolation And Identification of Some Species of Salmonella From Some Local Foods In Tikrit City

Author name: شيماء جابر حميد البياتي
Supervisor name: عبد الكريم فتاح عمر
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Salahaddin
First pages:
Abstract: تم التحري عن بكتريا السالمونيلا في 520 عينة من الاغذية المحلية في مدينة تكريت, وجدت هذه البكتريا في 26 عينة (5%), كانت اعلى نسبة لتواجدها في عينات اللحوم المثرومة (2.5%) والتي تمثل 16.25% من العينات الكلية للحوم المثرومة المدروسة.عزلت السالمونيلا من عي | The presence of Salmonella spp. was investigated in 520 local food samples in Tikrit city, Salmonella was found in 26 samples (5%), with the highest presence in minced meat (2.5%) which was represented 16.25% of all minced meat samples. Salmonella was isolated from chicken's meat at a percentage of 3.75%, and same percentages for meat slices samples, egg cortex and chicken's blood represented 5% for each one, and isolated too from vegetables and cheese at a percentage of 2.5% for each one. No Salmonella has been detected from sweet, yoghurt and egg content. Six Salmonella species were isolated, S. typhimurium was to be the most frequent and represented 42.30% of positive samples, then S. enteritidis and S. anatum in 34.61% and 11.53% respectively, but S. agona, S. typhi and S. arizona existed in a percentage about 3.84% for each one. Also, all the isolated species showed their own serological formula. The selective medium Tetrathionate broth (TTB) was found to be superior for isolation of Salmonella than Selenite cystine broth (SCB), the former positivity was 96.2% and 88.5% respectively. For test of better selective medium for isolation, TTB was found to be more suitable for isolation of S. anatum, while SCB was better for isolation of S. arizona.All the six isolated Salmonella species showed high sensitivity to Ciprofloxacin (5?g) and moderately sensitive to Chloramphenicol (30?g), while they showed high resistance to three antibiotics Amoxicillin (30?g), Nalidixic acid (30?g) and Pipracillin (100?g). They showed resistance with different percentages to the other tested antibiotics : Ampicillin (10?g), Gentamicin (10?g), Streptomycin (10?g), Tetracycline (30?g) and Trimethoprim (25?g).
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