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تاثير عدد من المستخلصات النباتية الطبية في تركيز هرمون الميلاتونين وبعض المتغيرات الفسلجية والنسجية في ذكور الجرذان البيض == Effect of Many Medical Plant Extracts In Melatonin Hormone Concentration And Some of Physiological And Histological Parameters In Albino Male Rats
Author name:
وداد محمود لهمود العبي
Supervisor name:
زيد محمد مبارك المهداوي | صالح محمد رحيم العبيدي
General topic:
Biology
Specific topic:
Zoology - Physiology
Degree:
Doctorate
University:
Tikrit University - Faculty Of Education - Department Of Biology
Language:
Arabic
University location:
Salahaddin
First pages:
24T2968 - p.pdf
Abstract:
صممت الدراسة الحالية للبحث في الاجهاد التاكسدي المستحدث بتاثير الجرعة العلاجية لعنصر الحديد لوحده بشكل املاح الحديد بهيئة اقراص Ferrous fumarat (2.85ملغم/كغم من وزن الجسم/يوم)التي تعادل (200ملغم /70كغم من وزن جسم الانسان /يوم)وقد بلغت كمية الحديد فيها (0. | The current study was designed to observe the oxidative stress induced by first dose for Iron salt alone as ferrous fumarat (2.85 mg /kg of b.wt./day)was equivalent to (200mg/70 kg of b.wt. of person)and it is containing of iron (0.94mg iron/kg of b.wt.), or by giving together doses of iron (ferrous fumarat) salt conjunction with doses of copper (in copper sulfate) in white male rats which has been given one of The experimental unit (8.57 mg/kg b.wt./day)was equivalent to (600mg / 70 kg of b.wt. of person) which use for treating acute anemia, in experimental group were given dose (8.57 mg/kg b.wt./day + 8.57 mg copper sulfat/kg b.wt./day) daily by the oral feeding and the dosage considered as the (first treatment group), with regard to a second dose of iron and copper were as follows (17 mg iron /kg b.wt./day +17 mg copper/kg b.wt./day) and considered a (second dose), research on the role of the aqueous extracts for number of plant commonly used in popular medicine like pomegranate (Punica granatum L.), green tea (Comellia sinensis L.) and virgin olive oil (Olea europaea L.) to limit or inhibit the accumulation of these salts in number of organic Tissues and normalization of the variables in the balance oxidants - antioxidants and a number of Biochemical and Physiological parameters in addition to the Histological Changes in Liver, Kidney and Testis of the white male rats used through 30 day of The experiment, The present study aimed to evaluate activity of many plant juice Like sour Cherry (Prunu scersus L) sweet Cherry (Prunu savium L.)sweet paprika (Capsicum annuum L.) and Banana (Musa paradisiacal L.) in number of Biochemical and Physiological variation, in addition to Histological changes in the Liver, Kidney and Testis in white male rats and oxidative stress induced by Hydrogen peroxide(0.5%) during the experimental period of 30. current study investigation included the effect of exposing the white male rats for continuous lighting and the constant darkness for a period (30 days).The present study aimed show the effect of Tryptophan experimental in most Physiological, Biochemical and Histological activities. The experiments had been performed in the animal house at Dept.of Biology of Education Fecundity at Tikrit Univ. from Jan. 2011 through July 2011. male rats were measured (250 - 350)gram, in age (16 - 20) weeks, (110) experimental, the rats were divided to (22) groups in which each consisted of (5) rats.The first experiment results have revealed the following : - 1 - The giving of Ferro alone and the Second dose has caused a noticed significant increase at the level of (p<0.01) in the total number of white blood cells (WBCs) and in the concentrations of each of Glucose(Glu), Total cholesterol (TC), Triglyceride (TG), Low density of lipoprotein - cholesterol (LDL - C), (VLDL - C), Malondialdehyde(MDA)and Peroxy nitrite(ONOO.) compared with control group, whereas it caused a high significant decrease at the level of (p< 0.01) in the concentration of each of High Density of lipoprotein of cholesterol(HDL - C), Total protein (TP), Glutathione(GSH) and ceruloplasmin(CP) and a decrease in sperm parameters. The two groups have also noticed a significant decrease of (p<0.01) in the concentrations of the melatonin hormone in the rats blood serum, growth Hormone and the Luteinizing hormone, in addition to the testosterone hormone and a decrease in the effectiveness of the catalase enzyme; taking into consideration the accumulative increase of Ferro element in the liver, kidneys and testes for both groups. As for the accumulation of copper, it has been noticed in the second dose group in liver, kidneys, and testes tissues, whereas the group of animals exposed to Ferro alone has noticed a high significant decrease in copper concentration in the three studied organs.2 - The giving of a first dose to white male rats (rattus ratus) has revealed a lack of significant differences in each of the total number of white blood cells, and the concentrations of most of biochemical parameters and sperm parameters, taking into consideration the significant decrease in the concentrations of each of (HDL - C), (GSH), (CP), (Mel), and (CAT). It has been noticed the occurrence of a significant increase in the concentration of (LDL - C). This group has also suffered a significant and insignificant increase in the Ferro accumulation in the studied organs, with a simple decrease of the accumulation of copper element in the three studied elements compared to the sound control group.3 - The giving of the three vegetal extracts which are Punica granatum L., Comellia sinensis L., and Olea europaea L. for animals exposed to second dose has caused a high significant decrease (p< 0.01). The effective power of extracts according to their arrangement and order in which first is Olea europaea L., then Punica granatum L., and lastly is Camellia sinensis L. have revealed positive significant changing in many biochemical parameters, with high significant increasing in (Mel), (GH), (LH), and (T) blood serum conc. in addition to the effectiveness of (CAT), compared to the group of animals exposed to second dose. All the sperm parameters in each of Olea europaea L. and Punica granatum L. have revealed a significant increase compared to the second dose group, whereas the Comellia sinensis L. has not revealed the occurrence of significant changes in most of the sperm parameters except in the percentage of active and moving sperms when compared with the second dose group.As for the accumulation of mineral elements, all the groups treated with vegetal extracts and exposed to second dose have witnessed a decrease in the accumulation of Ferro element in the liver, kidneys and testes tissues when compared with the second dose group, and this is also a fact concerning the accumulation of copper element.4 - The giving of Ferro alone and second dose have caused clear malady and tissue changes represented by diffusion of fat drops in a wide range between the liver cells. There has also been a poisonous and harmful effect of kidney tissue represented by the harm occurring in the disintegration and damage of the glomerulus and damage to the renal tubules, on the level of testis tissue, there have occurred huge damages in most tissue structures of the testes with a loss of most of the sperm formation stages, whereas the first dose group has not revealed any clear tissue changes compared to the sound control group. These changes have decreased when treated with vegetal extracts for each of Punica grantum L., Olea europaea L. and Comellia sinensis L. with the availability of Ferro and copper elements, since these elements have largely decreased the negative effects by affecting the three tissue levels which are liver, kidney and testes. Olea europaea L. has revealed a magnificent role on the reformative level of damaged tissues with a big similarity to the control group which exceeded both of Punica granatum L. and Comellia sinensis L. compared to the second dose group.Second experiment results : - 1 - The treatment of animals with hydrogen peroxide revealed very high significant changes at the level of (p<0.01), (p<0.05) in white blood cells, and in most of the biochemical and hormone criteria and sperm parameters which are similar to the effect of each of the Ferro dose alone and the second dose when compared with the sound control group.2 - The treatment of animals exposed to oxidative stress via hydrogen peroxide with Capsicum annuum L. and Musa paradisiacal L. separately Shown positive effect more than Prunus cersus L. and Prunus avium L. all juice led to the occurrence of noticed significant decrease at the level of (p<0.01) in the total number of white blood cells and in the concentrations of (Glu), (TC), (TG), (LDL - C), (VLDL - C), (MAD) and the root (ONOO.). This is compared with the group exposed to hydrogen peroxide alone, taking into consideration the occurrence of high significant increase in the concentrations of (HDL - C), (TP), (GSH), (CP) and in sperm parameters. There has also been noticed a significant increase in (p<0.01) in the concentrations of each of (Mel), (GH), (T) and (CAT)and (LH) compared to the group exposed to hydrogen peroxide alone.3 - The treatment with industrial tryptophan did not reveal any changes in the total number of most of the studied biochemical and hormone concentrations, but there has been witnessed an occurrence of significant decrease in the concentration of (LDL - C). As for the sperm parameters, the changes were between significant decrease and no significant changes when compared with the control group, with a significant increase in the effectiveness of enzyme CAT and concentration of hormone (Mel) in blood plasma with the sound control group.4 - The exposition of male rats (rattus ratus) to a period of continuous lightness led to the occurrence of several physiological and biochemical changes and to a high significant increase of (p<0.01) in sperm parameters and in the concentrations of hormones (Mel), (GH), (T), and (CAT) in blood plasma when compared with the control group; except for the occurrence of high significant increase in concentration of (LH). The darkness group revealed slight insignificant changes in most of the biochemical criteria and between the lack of changes in concentrations of (GH), hormone (T) and enzyme (CAT). This group also suffered the occurrence of high significant increase in concentration of (Mel) and a significant decrease in concentration of hormone (LH) when compared to the control group.5 - The giving (H2O2)caused several tissue changes in the livers of male rats (Rattus ratus). This has caused clear tissue and malady changes and similar to the Ferro and second dose groups. These changes have reduced when treated with Prunus cersus L., Prunus avium L., Capsicum annuum L. and Musa paradisiacal L. with the presence of hydrogen peroxide in that it greatly reduced the negative effects of hydrogen peroxide on the level of the three tissues of liver, kidney and testes. There is a lack of harmful tissue effects of industrial tryptophan on the tissues under study, when compared with the control group. In addition, both groups of rats exposed to durations of continuous lightness and darkness have suffered very huge tissue damage on the level of the three tissues, but the continuous lightness group suffered more damage when compared with the control group and continuous darkness duration.
