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دراسة تاثير مستخلصات نباتي القرنفل والخروع على نمو بعض الفطريات المنتجة لانزيم البروتييز والمسببة لالتهاب الاذن الخارجية == Study Effect of Syzigium Aromaticum And Ricinus Communis Extracts On Growth of Some Fungi That Produce Protease And Cause Otitis Externa
Author name:
بتول عمران ذيب
Supervisor name:
خليل ابراهيم بندر النعيمي | ذكرى احمد حمادة
General topic:
Biology
Specific topic:
Microbiology - Fungi
Degree:
Master
University:
Tikrit University - College Of Science - Department Of Biology
Language:
Arabic
University location:
Salahaddin
First pages:
24T2862 - p.pdf
Abstract:
ضمنت الدراسة عزل وتشخيص الفطريات من الاذن الخارجية في مستشفى تكريت التعليمي - وحدة الاذن والانف والحنجرة والعيادات الخاصة في مدينة تكريت, خلال الاشهر تموز, اب وايلول 2008. جمعت 100 مسحة اذنية من المرضى المشخصة اصابتهم بالتهاب الاذن الخارجية الفطري من قبل | The study was included isolation and identification of fungi from the external ear in Tikrit teaching hospital, the unit of Ear, Nose, and throat (E.N.T.) and Privete clinics in the city of Tikrit during the months July, august, and September2008. 100 ear swabs were collected from the patiants whose diagnosed by Otologists with Otomycosis, and 50 ear swaps were collected from healthy persons in the same city and period time, These samples were examined by dierect microscopic examination treated with KOH 10% with hot flame to ensure the fungal infections, and Culturing on Sabouraud, s Dextrose Agar was done for isolation and identification of types of fungi which responseple for infection, The result of direct microscopic examination and culture showed that 76(76%) were positive for fungi, 64 were positive by direct microscopic examination and culture, and 12 posative in culture only. The most common fungi isolated from patient were Aspergillus Spp.36). (%22 followed by Candida Spp. (%67. 10), Trichophyton Spp(%6.5) , and Penicillium spp. (%3.94%), Aspergills niger appeared as the predominant fungi (%84. 36) followed by Aspergillus flavus (%36. 22), Candida albicans (42. 18%), Aspergills terrous (7.89%) , Candida kruzi, Penicillium spp (3.94%), Trichophyton mentagrophyte, Trichophyton verrucosum (63. 2%) and Trichophyton scheonlieni (%1.31), and no fungi were isolate from the healthy person. Higher percentage of fungi infection were recorded in female 46(60.52%) than male 30(39.47%). and the higher percentage for fungal infection was recorded in the age group (31 - 40) with percentage (23.68%). fungal ear infection was recorded with highest percentage in right ear 47 (61.84%), and for the left ear 29 (38.15%). and high rate of infection was recorded in august (73.69%), Higher percentage of infection was recorded in patient house wives (52.36%), the itching was the most commen symptom , The most habits used to clean the ear by dearty body (51.31%), Ahigher percentage (76.4%) recorded in non educated persons. The results of active ingredients test for Syzigium aromaticum and Ricinus communis revealed that clove dry flower buds contain Flavonoides, Glycosides, Tannins, Volatile oils, Terpenes, Resins, and Phenols, while castor leaves contain Flavonoids, Tannins, Volatile oils, Terpenes, Resins, Saponins and Phenols, pH of clove bud 4.3 and pH of castor leaves 5.8. Aqueous and alcoholic extracts were Prepeared, which belongs to Syzigium aromaticum and Ricinus communis under the study, commercial oil for clove and seed castor were used in the study, the effects of inhibition of Aqueous and Alcoholic extracts and commercial oil had been studied on the growth and protease production on the three fungi which were showed the high ratio appeared among the isolation sample under the study with 5 concentration 10, 20, 30, 40, 50 (mg /ml), and the affected rate of these extracts and oils under the study on fungi were varied. The relation between Aqueous extract of clove bud and fungi growing was increasing relation, While Alcoholic Extract and commercial oil showed preference in the inhibition of the isolated fungi grown while its effect on Candida albicans was increase with increase the concentration, The effect of aqueous and Alcoholic Extract of castor leaves on Moulds showed reverse effect. on the other hand the commercial oil effect was slight effect, and both extracts did not showed effect on the Candida albicans as well as the Commercial oil.When adding Clove Extracts and Commercial oil and in all concentrations dose not gave any production of the Protease enzyme in the solid media, whereas the extracts of castor leaves and commercial oil were showed effects on the production of Protease enzyme, and Aspergills niger was more effected and these extracts did not showed an effect on the Candida albicans.Aqueous and Alcoholic extract and commercial oil to the Clove revealed higher influence rate than the effect of the Castor leaves extract , and in a different rate
