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التوصيف الجزيئي لبعض الفطريات الانتهازية المعزولة من المرضى الضعاف مناعيا واختبار حساسيتها تجاه مستخلص الثوم == Molecular Identification of Some Opportunistic Fungi Isolated from Immunocompromised Patients and Their Testing Sensitivity Against Extract of Garlic ( Allium sativum)

Author name: هند حبيب عبد الامير الفرطوسي
Supervisor name: ياس خضير عباس | ميثاق ستار عبود
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: تضمنت الدراسة الحالية العزل والتوصيف الجزيئي والمظهري لبعض الفطريات الانتهازية من القناة التنفسية السفلى للمرضى الضعاف مناعيا المراجعين لقسم الاورام في مستشفى الحبوبي والمراجعين للعيادة الاستشارية للامراض الصدرية في محافظة ذي قار (جنوب العراق) حيث تم جمع 130عينة قشع sputum للفترة من 1/10/2015 الى 1/5/ 2016 . بلغ عدد العينات الموجبة للزرع 85عينة وبنسبة 65.38% , اذ شكل عدد عينات الاناث الموجبة 21 عينة وبنسبة 46.67 % , اما عدد عينات الذكور الموجبة فقد بلغ 64 عينة وبنسبة 75.29% . ظهرت اعلى نسبة عزل عند الفئة العمرية 61 - 70 سنة وبنسبة 32.94 % بعدها الفئة العمرية 31 - 40 سنة وبنسبة 15.25 % . وتم الحصول على 106 عزلة فطرية . باستخدام طرق التشخيص المظهرية والجزيئية, بينت هذه الدراسة ان اعلى نسبة ظهور للفطريات المعزولة تمثلت بجنس spp. Candidaثم جنس Aspergillus spp.تلاه جنس Penicillium spp. بالنسب المئوية 49.41% 38.32 % 21.17 % وبالترتيب . كما ظهرت Candida albicans ثم Aspergillus flavus بالنسب المئوية 36.47 % , 12.94% بالترتيب, كما تم تسجيل 15 عزلة من الفطريات المعزولة في البنك الجيني الدولي . شملت الدراسة استخدام اربعة مذيبات عضوية وهي الايثانول, الاسيتون, الميثانول والماء بحيث كان قد استخدم من المذيبات الثلاثة غير الماء التراكيز 50%, 70% فضلا عن التركيز المطلق للحصول على مستخلص الثوم. اختبرت اربعة تراكيز من كل مذيب ضد 11 نوعا من الفطريات المعزولة, واظهر المستخلص المائي كفاءة جيدة في تثبيط جميع انواع الفطريات المدروسة بالمقارنة مع المذيبات العضوية الاخرى اذ بلغ اعلى معدل تثبيط عند التركيز400 مايكروغرام\مل بحيث ابدى الفطر Penicillium notatum تاثرا كبيرا للمستخلص المائي الذي بلغ اعلى معدل تثبيط له (60 مليمتر) عند التركيز المذكور . حددت الفعالية الحيوية لكل مذيب ضد كل الفطريات المدروسة وتم مقارنة المستخلص مع ثلاثة مضادات فطرية Miconazole وNystatin وAmphtericin B واظهر المضادان الاول والثاني تاثيرات متباينة اما المضاد الثالث فلم يظهر اي تاثير على الفطريات المختبرة . تم تحديد السمية الخلوية للمستخلص المائي الخام باستخدام كريات الدم الحمر ولم يتسبب في اي تحلل دموي. كذلك تضمنت الدراسة الكشف عن المركبات التي يحتويها المستخلص المائي للثوم باستخدام الكشوفات النوعية وتقنية كروماتوغرافيا الغاز GC فكشفت تلك الطرائق وتقنية كروماتوكرافيا الغاز بان المستخلص يحتوي على القلويدات والفينولات والفلافونويدات والتربينويدات والتانينات والكاروتينات بالاضافة الى وجود الاحماض الامينية ومركبات كبريتية اهمها الاليسين allicin واخرى اروماتية | The current study included isolation and molecular and phenotypic identification of some the opportunistic fungi from the lower respiratory tract of the immunocompromised patients in the Department of Oncology and Thoracic Consultant Clinic of Alhaboobi hospital at Thi - Qar Province ( South of Iraq). The specimens of sputum were 130 which collected during 1/ October /2015 until 1/May/2016. The study showed that 85 of the collected specimens appeared as positive results which were 65.38% of the ratio in which the females ones were 21 (46.67%) specimens while 64 (75.29%) for males. In addition that, the highest ratio of the positive results was recorded in the ages of 61 - 70 years, followed by 31 - 40 years which were 32.94% and 15.25%, respectively. Totally, 106 isolates of the isolated opportunistic fungi appeared from the positive specimens. Through using morphological methods and molecular techniques, this study showed the first rank of the isolated fungi was Candida spp followed by Aspergillus spp, and Penicillium spp which possessed ratios of 49.41%, 38.32% and 21.17, respectively. According to the species, the first ratio of the isolation was Candida albicans followed by Aspergillus falvus, as 36.47%, 12.94%, respectively. Fifteen fungal isolates of the present study were recorded in the Gene Bank of NCBI. In this study, the extracts of Allium sativum were separately obtained by using four organic solvents (ethanol, acetone, methanol and water) and three concentrations (50%, 70% and absolute) of each solvents except water. Four concentrations of each extract were tested against 11 species of the isolated fungi in which the water extract gave the best value of the bioactivity including highest inhibitory value (60 mm) against Penicillium notatum by using 400µg\ml compared with the extracts of ethanol, acetone, and methanol. The bioactivity of Allium sativum extracts of each solvent was done against the tested fungi in comparison with Amphotericin B, Nystatin and Miconazole in which two of them produced the different bioactivities while Miconazole had no effect. The blood cytotoxicity of the crude water extract was performed against red blood cells and it did not appear any hemolysis. The chemical tests and Gas Chromatography (GC) revealed that the water extract contains alkaloids, phenols, flavonoids, terpenoids, tannins and carotenes in addition to sulfur formations, such as allicin as well as aromatic compounds.

التحري الجزيئي والوبائي لطفيلي المتحولة Entamoeba Spp. في الاطفال المصابين بالاسهال في محافظة ذي قار == Molecular and Epidemiological investigation of the Parasite Entamoeba Spp. In children with diarrhea in Thi Qar Province

Author name: منار كريم كاظم السعيدي
Supervisor name: فاضل عباس منشد العبادي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: شملت الدراسة الحالية فحص 3909 عينة غائط للاطفال دون سن 12 سنة الذين يعانون من الاسهال ويشكون من الام بطنية والمراجعين لمستشفى بنت الهدى التعليمي ومستشفى الشهيد محمد الموسوي والمراكز الصحية والعيادات الخارجية في محافظة ذي قار خلال المدة من شهر تشرين الاول 2015 لغاية شهر ايلول 2016 تضمنت الدراسة ثلاثة محاور رئيسة . المحور الاول هو تشخيص جنس المتحولة مجهريا باستعمال المسحة المباشرة الرطبة وطريقة التركيز وذلك للكشف عن الاطوار المتغذية والمتكيسه للطفيلي واظهرت اصابة كلية بجنس المتحولة Entamoeba spp. بنسبة 26.86% واختيرت 80 عينة من العينات الموجبة مجهريا لتاكيد التشخيص باستعمال الفحص الجزيئي PCR بواسطة الجين 18S rRNAفسجلت الدراسة اصابة كلية بطفيلي المتحولة بنسبة 35% ، اظهرت نتائج الفحص باستعمال طريقة Nested PCR لتمييز الانواع (Species Specific) اصابة بطفيلي المتحولة الحالة للنسج Entamoeba histolytica بنسبة 39.29% وبطفيلي المتحولة المتغايرة Entamoeba dispar بنسبة 39.29% . فحصت العينات الموجبة (28) للPCR باستعمال Real - Time PCR وبوجود المجسان Probes المجس Histolytica - 96 T الخاص بالمتحولة الحالة للنسج والمجس Dispar - 96 T الخاص بالمتحولة المتغايرة وذلك لتاكيد التشخيص وتمايز الانواع فثبت وجود المتحولة الحالة للنسج باعلى نسبة 53.57% والمتحولة المتغايرة بنسبة 39.29% والانواع الاخرى لجنس المتحولة بنسبة 7.14% وبفارق معنوي بين الطريقتين الجزيئيتين مع وجود تاثير معنوي لنوع الطفيلي في احداث الاصابة . اظهرت الدراسة اصابة مفرد لطفيلي E. histolytica بنسبة 45.50% ولطفيلي E.dispar بنسبة 27.20% فيما سجلت اصابة مختلطة بنسبة 27.30% كما وسجلت وجود كريات الدم الحمر في 80% من العينات الموجبة لوجود E. histolytica وبنسبة 54.55% من العينات الموجبة لوجود E.dispar . تضمن المحور الثاني دراسة انتشار الاصابة بجنس المتحولة من خلال دراسة علاقة الاصابة مع بعض العوامل الوبائية ، منها فصول السنة التي اظهرت وجود فروق معنوية P≤0.05 فكان فصل الصيف اعلى الفصول من حيث نسبة الاصابة 37.06% وادناها في فصل الشتاء بلغت 14.14% ، كذلك لم تبين النتائج وجود فروق معنوية للاصابة فيما يخص الفئات العمرية فكانت النسب اعلاها بين الاطفال دون (3) سنوات فبلغت 30.50% ادناها بين الاطفال في الفئة الاعلى من (10) سنوات بلغت 17.13% ، لم تسجل الدراسة اية فروق معنوية في نسبة الاصابة بالطفيلي بحسب الجنس فسجلت نسبة 26.87% و26.85% للاناث والذكور على التوالي، في حين اظهرت الدراسة وجود فروق معنوية ملحوظة فيما يخص المستوى المعاشي فكانت نسبة الاصابة لدى الاطفال الذين ينتمون للعائلات ذات الدخل المحدود هي الاعلى 37.98% واقلها للذين ينتمون للعائلات ذات الدخل فوق الجيد وبلغت 10.11% كما سجلت النتائج وجود فروق معنوية في نسب الاصابة حسب المستوى التعليمي للابوين فكانت نسبة الاصابة عالية لدى الاطفال الذين المستوى العلمي لذويهم دون الابتدائية مقارنة بذوي الاطفال من حملة الشهادات الجامعية وبلغت النسب 36.85% و8.55% على التوالي فيما يخص عدد افراد العائلة كانت نسبة الاصابة عالية وبفارق معنوي لدى الاطفال الذين ينتمون لعائلات تتكون اكثر من (10) افراد واقلها للذين ينتمون لعائلات التي يبلغ عددها (5 - 3) افراد بنسب بلغت 36.95% و8.54% على التوالي . تضمن المحور الثالث دراسة بعض المعايير الدمية ومعرفة التغير في نسبة هذه المعايير لدى الاطفال المصابين بالطفيلي عند مقارنتها بالمعايير الدمية للاطفال الاصحاء وتم ذلك باستعمال جهاز Coulter Counter . جمعت 150 عينة دم من الاطفال المصابين بطفيلي المتحولة و50 عينة دم من الاطفال الذين يمثلون مجموعة السيطرة الاصحاء وتبين وجود فروق معنوية واضحة في نسبة خضاب الدم 10.16%وحجم كريات الدم الحمر المضغوط 34.7% حيث لوحظ انخفاضهما في مجموعة المصابين مقارنة بالقيم في مجموعة السيطرة التي بلغت 12.34 و39.10 على التوالي. سجلت نتائج العد الكلي لخلايا الدم البيض وجود ارتفاع معنوي في الاطفال المصابين مقارنة بمجموعة الاطفال الاصحاء وبلغت 11.3، 8.37 على التوالي وفيما يخص العد التفريقي لها فقد بينت النتائج وجود ازدياد بفارق معنوي في عدد الخلايا العدلة 6.92 و4.03 والخلايا الحمضة 0.61 و0.17 والخلايا الوحيدة 1.19 و0.93 على التوالي ولوحظ كذلك وجود انخفاضا معنويا في عد الخلايا اللمفاوية في الاطفال المصابين مقارنة بقيمتها لدى اطفال مجموعة السيطرة التي بلغت 2.19 و2.76 على التوالي | The current study included examination of 3909 stool samples from under 12 years old children who visited Bint Al - Huda hospital, Martyr Mohammad Al - Mosawi hospital, medical centres and outpatient clinics in Thiqar governorate during the period from October 2015 until September 2016. All those individuals were suffering from diarrhea and abdominal pain. This study included the microscopic identification of Entamoeba Spp. using direct wet amount and concentration techniques in order to diagnose both Trophozoite and cyst stages of the parasite. The result revealed that 1050 (26.86%) of the collected samples were infected with Entamoeba Spp. Out of these infected samples, 80 were randomly selected for further confirmation by conventional PCR and 18S rRNA. Using this method, 28 (35%) was recorded to have Entamoeba Spp. Positive samples from conventional PCR were further examined by Nested PCR for species specific identification. The result of this method showed the presence of two species, E. histolytica (39.29%) and E. dispar (39.29%).Positive samples for molecular examination (28) were then examined by Real time PCR in order to verify and confirm the molecular results. Two probes were used, Histolytica - 96 Tfor E. histolytica and Dispar - 96 T for E. dispar. A significant difference was observed between two methods. E. histolytica was found to be higher percentage followed by E. dispar and then Entamoeba Spp., 53.57%, 39.29% and 7.14% respectively. The Real time PCR reported mono - infection with E. histolytica in 45.5% and with E. dispar in 27.2%, whereas reported mixed infections by both parasites in 27.3%. Red blood cells (RBCs) were detected in 80% E. histolytica positive samples and in 54.55% E. dispar positive ones.The environmental factors and their correlation with Entamoeba Spp. prevalence were also included in this study. The infection prevalence based on the seasonal variation showed significant differences P≤ 0.05. Summer recorded the highest infection prevalence whereas the lowest was in the winter, 37.06% and 14.14% respectively. Regarding the infection in age groups, the highest percentage (30.5%) was recorded in under 3 years old group and the lowest (17.13%) was found in above 10 years old one. However, no significant difference P≤ 0.05 was observed between these age groups. Furthermore, no significant differences were recorded betweenpatient’s sex. Entamoeba Spp. infection (37.98%) appeared to be higher in children who belong to families with low standard of living compared to those who are from families that earning a higher income (10.11%). A Similar pattern was found in Entamoeba Spp. prevalence based on the number of the patient’s family members. Higher prevalence and significant difference were reported (39.95%) in patients who belong high family member (above 10), compared to those from 3 - 5family member (8.54%).One hundred and fifty blood samples from infected children with Entamoeba Spp. and fifty samples from healthy children as a control were collected during the present study in order to observe the change in blood picture and parameters between two groups. Significant differences were reported in blood hemoglobin (10.16%) and volume of packed RBCs (34.7%) in the infected group compared to 12.34% and 39.10% in the control group. Total leukocyte count was found to be significantly higher in the infected group (11.3) compared to the individuals in the control group (3.37). Differential leukocyte count also showed significant differences in Neutrophils (6.92%, 4.03), eosinophils (0.61, 0.17), monocytes (1.19,0.93) and lymphocytes (2.19, 2.76) in infected and control groups respectively.

تاثير الاصابة التجريبية بطفيلي المقوسة الكوندية Toxoplasma gondii وبعض الادوية على خصوبة الجرذان المختبرية Rattus norvegicus == Effect of experimental Toxoplasma gondii infection and some drugs on fertility rats Rattus norvegicus

Author name: زهراء سعدون هادي الغزي
Supervisor name: فاضل عباس منشد العبادي | علي اسماعيل عبيد السنافي
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: This study included 3 stages. In the first stage, Toxoplasma gondii was isolated from aborted women placenta who visited Bent Al - Huda hospital and Al - Shatra hospital after confirmation of the prescence of parasite by impression smear method. Then parasite uspension injected 0.3 ml which contain 100 cyst intraperitoneally (174 rat) to 87 male and 87 female rats, inaddition to control group 30 rat (15 male and 15 female). After 10 days of injection, diagnosed the acute infection by Real - Time Poplymerase Chain Reaction(Real - Time PCR) and showed 97.70% (170 infected rat and 4 noninfected rat) , in male 96.55% (84 infected rat and 3 noninfected) ,in female 98.85% (86 infected rat and 1 noninfected) . After 2months of infection 84 animals (42 males and 42 females)were sacrificed, dissected (brain, testis and ovary ) were isolated to confirm the occurance of chronic infection with the using Real - Time PCR. The incidence of infection was 100% in brain males and ovary females and recorded 71.43% in testis and 90.48% in brain females. So diagnosed infection by impression smear method, identified the amount of DNA of the parasite T. gondii by using Real - Time PCR. The use of the remaining number of animals within the following experiments.The second stage included effect of experimental Toxoplasmosis infection and some drugs on fertility 168 rat (84 male and 84 female). Animals were subdivided into 6 groups for each group included 14 rat : (First group) non infected treated by DMSO, (Second roup) non infected treated by Sulfadiazine, (Third group) non infected treated by Pyrimethamine, (Fourth group) infected treated by DMSO, (Fifth group) infected treated by Sulfadiazine and (Sixth group) infected treated by Pyrimethamine.The current study results revealed prominent effect of T. gondii infection on the hormonal level in both males and females. T. gondii infection induced significant decline in Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH) and Testosterone levels in nfected male compared with non infected males treated by DMSO. Sulf. and Pyr. treatment significantly elevated the level of these hormones (P <0.01) in infected males compared with fourth group similar to levels its in first group. Toxoplasmosis in female rats significantly decrease FSH, LH, Estrogen and Progesterone levels in compared with first group, while treating of infected females with Sulf. and Pyr. didn,t improve the declined levels of these hormones in infected and noninfected females comparedwith fourth and first group, respectively.The study showed that Toxoplasmosis in male rats induced significant decline in the sperm count in the epididymal cauda, sperm viability and relative weights of male sexual organs with an increase in sperm malformation ratio in infected male compared with first group. Treatment with Sulf. and Pyr. in infected male significanthy elevated the sperm count and viability (P<0.05), compared with fourth group. Sulf. didn,t induced further significant changes, but Pyr. induced further significant increase in sperm malformation ratio in infected (P< 0.01) and non infected (P<0.05) rats. The study also revealed that Toxoplasmosis significantly decrease the relative weight of ovary and uterus compared with first group, while treatment with Sulf. and Pyr. increased the declined relative weight of the ovary and uterus in the infected females.Testicular histological section of Toxoplasmosis infected male rats treatment with DMSO showed vacular degeneration of spermatogonia and spermatocytes. The seminiferous tubules revealed sever degeneration, shrinkal, necrosis, hemorrhage. Seminiferous tubules lumen contained little amount of sperm with appearance of gaint cell, polymorphonuclear leukocytes and exfoliated cellular debris. Metamorphosis and disintegration of the tissue between the seminiferous tubules was also observed compared to the first group that showed complex histologically intact while testes sections of infected rats treatment Pyr. and Sulf. showed improvement by removing histocompatibility damage caused by the parasite infection.The epididymal sections of T. gondii infected rats treatment with DMSO showed infiltration of mononuclear and multinucleated gaint cell. Sections also showed hyperplasia of duct lining and contained tissue cyst of T. gondii. All these changes were improved in infected males treated by Sulf. and Pyr. Ovary sections of the infected females treatment with DMSO compared with first group revealed decreased primary and secondary follicle increased follicle atresia. Histological sections of ovary females showed in the fifth and sixth group to increase the number of primary and secondary follicles and decreased number of follicle atresia compared overian sections for the fourth group. Uterine sections of the infected females treatment with DMSO showed hypertrophy, increase endometrial thickness and myometerium, increase polymorphonuclear cell infiltration, fewer glands and congestion of blood vessels compared with first group. Histological sections for ovary females of the fifth and sixth group showed changes but less severe than is present in the fourth group.In third stage from studying Toxoplasmosis on fertility parameters of male and female rats, 84 rat(42 male and 42 female) which showed the results of the current study, when paired fertilized healthy females by males is infected and infected of T.gondii treatment with Sulf. , Pyr. and DMSO, and inversion, there is the effect of the parasite and used drugs the percentage of getting pregnant and pregnancy size and rate of embryos weight and placental weight and increase the proportion of fetal resorption and the proportion of early loss of embryos in fertilized healthy females by males five groups compared to the first group.Based on that conclude from the study that the infection of parasite T. gondii had negative effects on the fertility of male and female, and that all of thePyr. and Sulf. were not honest is the other of the impact on reproductive functions.

دراسة جزيئية ومناعية لالتهاب الكبد الفيروسي نمط جـ لمرضى الثلاسيميا في محافظة النجف الاشرف - العراق == Molecular and immunological study for Hepatitis C virus in Thalassemia patients in AL - Najaf AL - Ashraf / Iraq

Author name: ناديه حبيب سرحان الشيباني
Supervisor name: موسى نعمة مزهر | جميل جري يوسف
General topic: Biology
Specific topic: Microbiology - Viruses
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: This study was conducted to detect the infection percentage of Hepatitis C Virus (HCV) in the samples of thalassemic patients in AL - Najaf Governorate.About 130 suspected cases attending AL - Zahra'a hospital for children and delivery in Al - Najaf city during period from 1st of November (2014) to 28thof April (2015).Only 69 samples were carried out in this study .The practical side for this study followes three main paths : 1 - The Molecular identification of the presence of Ribonuclic acid of Hepatitis C virus in Thalassemic patients by using RT - PCR.2 - Using the Enzyme Linkedimmunosorbant assay (ELISA - III)technique to determinate immunological markers anti - HCV IgG and interleukine - 28B (IL - 28B).3 - Using theCYANHemato devicefor the purpose of counting blood cells which is called Coulter - Method to give a Complete blood count(CBC).The overall results obtained in this study were as follows : - Out of 69 samples,25(36%) samples of thalassemic patients were infected with Hepatitis C virus, 16 male and 9 female ,their age range from (4 - 43) years ,while 44(64%) samplesof thalassemic patients were withoutHepatitis C virus infection , 22 male and 22 female, their age range from (3 - 35) years . - The result showed that 25samples seropositive to anti - HCV IgG in all age groups where the age group (10 - 20) years old has thehighest percentage among other age groups. - The result of the examination of HCV - RNA Eleven of (25) HCV seropositive patients were positive to RT - PCR technique. - The result of this study showed that level of Interleukin - 28Bin thalassemic patients with HCV was high (mean or significant) (12733.33) pg/ml as compared with thalassemic patients without HCV (270.0463) pg/ml and control(12.1267)pg/ml. - The result also has revealed that the hematological parameters of white blood cells (WBCs),monocytespercentage (MON) and lymphocytes percentage (LYM) significantly have increased (P<0.05) in Hepatitis C patients compared to those of control group. - Granulocytes(GRA) showed a significant decrease (P<0.05) in percentage in Hepatitis C patients compared to those of control groups.

تنقية جزئية وتوصيف Biosurfactant من بكتريا Bacillus subtilis وتاثيرها على نمو بعض عزلات البكتريا والفطريات

Author name: غـفـران فـلـيـح عـبد الـحـسـن عـزيـز الجـبـوري
Supervisor name: فاطمة عبد الحسين مجبل التميمي
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: تم الحصول على 102عزلة من العزلات البكتيرية من 40 عينة من عينات الترب الملوثة بالنفط من مختلف المناطق ورشة عمل السيارات، محطات وقود السيارات، مصافي النفط ولخمسة مناطق مختلفة في العراق . خضعت كل هذه العزلات الى الفحص المجهري والخصائص الزرعية ووجد ان 80 عزلة منها تنتمي الى جنس Bacillus ، هذه العزلات خضعت ايضا لاختبارات بايوكيميائية وشخصت بناءا على دراسة rDNA 16S من قبل مجموعتين من البرايمرات (andB16SF B16SR) و(1492R and 27 F) اذ وجد ان 51 عزلة منها تعود الى Bacillus subtilis . تم فحص جميع هذه العزلات من حيث قدرتها على انتاج biosurfactant عن طريق فحص النشاط انحلال الدم على وسط دم الاغنام حيث وجد ان جميع العزلات كانت من النوع تحلل الدم بيتا حيث تم فحصها اعتمادا على قياس الشد السطحي بعد زراعتها في Jacques medium اظهرت النتائج ان 46 عزلة من 51 عزلة كانت منتجة biosurfactant , وقد تم اختيار ثمانية منها تميزت بانتاجها لهذا المركب وهي (S1,S2,S3 S4,S5,S6,S7,S8) حيث كانت العزلة B. subtilis S4 هي الاكفا في الانتاج لذلك اختيرت في الدراسات اللاحقة . تم استخلاص Biosurfactant الخام من العزلات المختارة بواسطة وتنقيته جزئيا باستخدام طريقة Ion exchange chromatography باستخدام DEAE - Cellulose تلاها طريقة Gel filtration chromatography باستخدام Sepharose - 6B . تمت دراسة خصائص المركبات المنقاة واظهرت النتائج ان الوزن الجزيئي biosurfactant التي تنتجهاB.subtilis S4 تراوح بين (1036 - 1058دالتون) من خلال جهاز HPLC - TOF - MS . اظهرت النتائج ان الحد الاقصى لانتاج biosurfactant من العزلة تم بطريقة استخدام زيت الزيتون (3٪) وكبريتات الامونيوم (0.3٪) وKH2PO4 (1.5) غم / لتر، وبدرجة حرارة 30 مº والاس الهايدروجيني (7) وفترة الحضانة لمدة 72 ساعة. وقد تحققت اعلى نسبة للـ biosurfactant بعد مكملات الظروف المثلى الى 0.3 غرام / لتر من الوسط الزرعي . تم اختبارالنشاط البكتيري والفطري للعزلات الثمانية ضد بعض الجراثيم المسببة للامراض، وهي Staphylococcus aureus,Streptococcus pneumonia, Pseudomonas aeruginosa ,Escherichia coli ,Proteus mirabilis, Candida albicans , Candida tropicalis , Candida krusei , Rhizopus oryzae , Aspergillus niger وكانت العزلة الاكثر فعالية هي B.subtilis S4 التي اظهرت وجود نشاط ضد كل مسببات الامراض ماعدا S.pneumonia ,P.aeruginosa ,C.krusei . كما بينت النتائج ان مستخلص العزلة B.subtilis S4 اظهر اعلى تثبيط نموعند التركيز 1500 مايكروغرام / مل وصل الى 24 ملم ضد E.coli، 23 ملم ضد K.aerogenes، 21 ملم ضد S.aureus، 15 ملم ضدC.albicans، 14 ملم ضد P.mirabilis، 11 ملم ضد C.tropicalis، وتثبيط نمو الفطريات، حيث وصل معدل النمو الشعاعي الى 31.9 ملم ضد A.niger و9.7 ملم ضد R.oryzae عند التركيز 2 ملغم / مل. كذلك اظهرت النتائج ان سمية المستخلص B.subtilis خارج الجسم الحي على ذكور الجرذان البيض بلغت حوالي 922 ملغم / كغم وتشير اذ ان الاعطاء اليومي biosurfactant لم تظهراي حالة وفاة في اي جرعة ، ولم تلاحظ اي تغييرات غير عادية في السلوك اوتاثيرات سمية خلال فترة 28 يوما من العلاج. واثبت التحاليل انه ليس هناك اي تاثير سلبي على معايير الدم الفسلجية وهذا يؤكد صحة هذه المستحضرات الحيوية للاستخدام . | About 102 bacterial isolates were obtained from 40 oil contaminated soil samples from various automobile work shop ,cars fuel stations ,oil refineries of five different areas in Iraq. Microscopic examination and cultural characteristics for all isolated were investigated , 80 isolates were found to be related to the genus Bacillus , Bacillus isolates were subjected to the biochemical tests and molecular identification based on the 16S rDNA by two sets of primers (B16SF and B16SR) , (27F and 1492R) of these 80 isolates revealed that 51 of the identified isolates belonged to Bacillus subtilis.All these isolates were screened for their ability for biosurfactant production through blood haemolysis activity on sheep blood agar indicated that all isolates were β - hemolysis while screening depended on measuring the lowering surface tension of cell free supernatant after cultivation in jacques medium which referred that 46 of 51 isolates were biosurfactant producers. Eight isolates were selected for the best biosurfactant producer isolatesCrude biosurfactants produced by the selected isolates were extracted by acid precipitation followed by partially purified with dichloromethane followed by purification and characteristics, the results showed that the molecular weight of biosurfactant produced by B.subtilis S4 ranging between 1036 - 1058 Da . Optimum conditions for biosurfactants production from B. subtilis S4 were determined. Results showed that maximum biosurfactant production from this isolate was achieved by using 3% olive oil , 0.3 % ammonium sulfate and 1.5 g / l Potassium dihydrogen phosphate ,at pH7,30 ºC and incubation period for 72 hrs. Maximum biosurfactant production was achieved after the supplementation of the optimum conditions was 0.3 g/l of culture medium .All of the eight isolates were tested for antimicrobial activity against twelve pathogenic microorganisms : Staphylococcus aureus ,Streptococcus pneumonia,Pseudomonas aeruginosa ,Escherichia coli ,Proteus mirabilis Klebsiella aerogenes,Candida albicans,C.tropicalis,C. krusei , Rhizopus oryzae , Aspergillus niger and Penicillium notatum.The most effective isolates were B.subtilis S4 which showed antimicrobial activity against some tested pathogens except S.pneumonia ,P. aeruginosa ,C.krusei and P.notatum.The results indicated that S4 culture extract showed high growth inhibition zone in concentration 1500 µg /ml reached to 24 , 23, 21, 15, 14 and 11 mm against E.coli , K.aerogenes , S.aureus , C.albicans, P.mirabilis,C.tropicalis respectively and high reduction in of radial growth of the fungi reached to 31.9 and 9.7 mm in A.niger and R.oryzae respectively at 2 mg /ml concentration .Study in vivo potential toxicity of B.subtilis lipopeptide biosurfactant towards male rat was evaluated. An LD50 value was determined to be about 922 mg / kg. Results show that daily administration of biosurfactant did not show any death cases at any dose. Also, no unusual changes in behavior and no intoxication were observed during the 28 days period of treatment. Analysis proved that there were no significant adverse effect on hematological parameters and this confirms the validity of those biopharmaceuticals on body of mammals.

دراسة جزيئية ومناعية لفايروس النورو والفايروس الغدي نمط 40 و41 في الاطفال المصابين بالاسهال == Molecular and Immunological Study of Norovirus and Adenovirus types 40 and 41 in Diarrheic Children

Author name: رمله احمد محمد حسن العميدي
Supervisor name: مهدي حسين محيل العمار
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: English
University location: Najaf
First pages:
Abstract: هدفت هذه الدراسة الى التحري عن فايروسي النورو والفايروس الغدي نمط 40 / 41 في الاطفال المصابين بالاسهال واجراء تحليل لمعدل الاصابة وطبيعتها ودراسة الطبيعة الجزيئية للفايروسين وكذلك التحري عن قدرة الفايروسين على حث الجهاز المناعي وتحديد بعض الحركيات الخلويه عند الاطفال المصابين بالفايروسين.بالاضافة الى دراسة بعض ملامح الاصابه مثل تغاير الفصول والعمر وتحديد افضل الطرق لتشخيص الفايروسين. اجريت الدراسة الحالية في محافظة النجف الاشرف /مستشفى الزهراء التعليمي للولادة والاطفال خلال الفترة من كانون الثاني2014 الى كانون الاول 2014 والتي تضمنت 314 عينه من البراز والدم من الاطفال المصابين بالاسهال والذين تتراوح اعمارهم بين شهر الى خمسة سنوات.حيث اعتمد التحري في عينات البراز استخدام اختبار الكروماتوغرافيا المناعي السريع للكشف عن المستضدات الفايروسية للفايروسين النورو والغدي نمط 40 / 41.كما تم تاكيد العينات الموجبة بواسطة تقنية PCR Real Time لفايروس النورو تقنية PCR للفايروسالغدي نمط40 / 41،والتي اظهرت فيها النتائج بان معدل الاصابة كان 11.7% لفايروس النوروو8.9% للفايروس الغدينمط 40 / 41.كذلك تم فحص العينات الموجبة بواسطة المجهر الالكتروني والتي بينت مظهر للفايروسين في عينات البراز . وتشير الدراسة الى ان التقنيات الجزيئية المستخدمة في فحص العينات هي الاكثر كفاءة للتشخيص الروتيني للفايروسين مقارنة مع المجهر الالكتروني لتشخيص عينات الاسهال.وطبقا للفئات العمرية ،اظهرت النتائج ان الفئة العمرية 13 - 24 شهر سجلت ارتفاع عالي للاصابة بالفايروسين النورو والغدينمط 40 / 41.الحالات الايجابية كانت بين الاطفال الذين يتغذون على الحليب الجاف الاصطناعي (31 حاله) مقارنة مع الرضاعة الطبيعية من الام (14 حاله).اما نتائج الاعراض السريرية في الاطفال المصابين بالفايروسين فاظهرت ان كل الحالات تبين وجود اسهال من النوع المائي المخاطي وبنسبة 100% يليها الالم البطن بنسبة 94.5% و82.1 %،التقيؤ بنسبة 81 % و78.5 % واخيرا الحمى بنسبة 78.3% و71.1 % لكلا من النورو والغدي نمط 40 / 41 على التوالي. كما كشفت الدراسة الحالية بان معدل الاصابة المرتفع لفايروس النوروكان من شهر كانون الثاني الى اذار والتي كانت بنسبة 81%من الحالات الموجبة ، اما بالنسبة لفايروسالغدينمط 40 / 41كان معدل الاصابة المرتفع من شهر كانون الثاني الى نيسان والتي كانت بنسبة 89.2% من الحالات الموجبة. كما اظهرت النتائج وجود ارتفاع كبيرفيالحركيات الخلويه (السايتوكينات) لمصل الاطفال المصابين مقارنة مع السيطرة. وفقا للفئات العمرية ،اظهرت الفئة( 49 - 60) شهر ارتفاع عالي في مستويات الانترفيرون كاما ، الانترلوكين - 6((IL - 6 والانترلوكين - 12 (12 IL - (حيث كانت للفايروس النورو(90.8, 56.7, 84.3) جزء من الغرام /مل على التوالي،بينما كانت لفايروسالغدي(106.5, 59, 87.5)جزء من الغرام /ملعلى التوالي مقارنة مع مجموعة السيطرة. | Norovirus and adenovirus types (40 and 41) are the important causes of gastroenteritis infections in children worldwide. The present study was designed to determine the incidence and nature of infection of norovirus and adenovirus types (40 and 41) at molecular and immunological levels, as well as some features such as seasonal variation , age distributions and detected the most effective and suitable method for diagnosis of the two viruses . The study was carried out in Al - Najaf province, in AL - Zahra teaching hospital for maternity and children, during the period from January, 2014 to December, 2014. Stool and blood samples were collected from 314 children with diarrhea, their age range (from 1months to 5 years). Immunochromatographic assay for norovirus and adenovirus used to detect their antigens in stool samples, the positive results were confirmed by reverse transcriptase real time PCR technique for norovirus and polymerase chain reaction technique for adenovirus types (40 and 41), which showed incidence of 11.7% and 8.9% for norovirus and adenovirus, respectively. Transmission electron microscopy examination gave clear positive norovirus and adenovirus types (40 and 41) appearance in diarrheal stool samples .The study indicates that molecular methods are most suitable and sufficient tests for routine diagnosis of norovirus and adenovirus types (40 and 41) as compared to transmission electron microscopy analysis for diarrheal samples. According to age groups, the results showed that the majority of infections with norovirus and adenovirus were recorded among age group (13 - 24) months. The positive cases were highly among infants fed with bottle fed (31 cases) compared with infants fed with breast fed (14 cases). Regarding the clinical features of children with viral diarrheal infections , the results showed that all of the cases of both viruses complained of watery or mucosal consistence stool (100%) followed by abdominal pain (94.5%) and (82.1%), vomiting (81%) and (78.5%) , and fever (78.3%) and (71.4%) for norovirus and adenovirus positive children respectively. The present study also revealed that a higher incidence of norovirus was detected from January - March 2014, when it was found in 81% (30/37) of the positive cases, whereas the incidence of adenovirus was higher from January - April 2014, during which it was found in 89.2% (25/28) of the positive cases. The current study for cytokines profile detection showed that significant elevation in patients with norovirus and adenovirus than control. The age groups 49 - 60 months have shown a high level in cytokines Interferon gamma, Interleukin - 6 and Interleukin - 12 with concentration were (84.3, 56.7, 90.8) pg./ml , respectively for norovirus while adenovirus were (87.5, 59, 106.5) pg./ml , respectively as compared with control group . Conclusion of the study shows incidence norovirus genotypeII (GII) and adenovirus types (40 and 41) by rapid chromatographic test from infected children in Al - Najaf province. The study indicates that molecular techniques are most suitable and sufficient tests alternatively for transmission electron microscopy when there is good facility and both can replaced by rapid chromatographic test . Interferon gamma, Interleukin - 6 and Interleukin - 12 were increased in all patients with norovirus and adenovirus infections and showed high level in age group (49 - 60) months

دراســـــة بعض المعايـــــير الجزيئيـــــة والمناعيـــــة لبكتيريا Staphylococcus aureus المقاومة للمثيسيلين والمعزولة محليا من الاصابات الجلدية المكتسبة مجتمعيا

Author name: هدى سعد عزاوي
Supervisor name: سلمان عزيز | سميرة كاظم
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Najaf
Key words:
  • المكورات العنقودية الذهبية
  • الخراج ونضح الجروح
  • الاصابات الجلدية
First pages:
Abstract: تعد البكتيريا العنقودية الذهبية المقاومة للمثيسيلين المكتسبة مجتمعيا من الكائنات المجهرية المرضية التي تسبب طيفا واسعا من الاصابات في مختلف بقاع العالم في اماكن مختلفة من جسم الانسان اذ اقترنت امراضيتها مع اصابات الجلد والانسجة الرخوة . تهدف هذه الدراسة الى التعرف على مدى انتشار هذه البكتيريا في حالات اصابة انسجة الجلد المختلفة ,واجراء تقييم جزيئي لهذه البكتيريا المعزولة من حالات مرضية مختلفة , وتقييم مستوى بعض الحركيات الخلوية في مصول المرضى . وقد اشتملت على 169 عينة اخذت من المرضى المصابين بالتهابات الجلد المختلفة كـ الدمامل والخراج الجلدي والجروح والقدم المصابة بالقرحة لدى مرضى السكري وعدوى الحروق والحصف (القوباء) واخذت من المرضى الذين يراجعون العيادات الخارجية , والمراكز الصحية , وكذلك العيادات الخاصة, لمدة امتدت اكثر من خمسة اشهر , وللفترة من تشرين الاول 2014 م وحتى اذار2015 م. تضمنت الدراسة الحالية اخذ مسحات من الجلد المصاب بالالتهابات المرضية المختلفة وعينات دم ,من بين 169 عينة ,76 عزلة فقط اثبتت عائديتها الى جنس المكورات العنقودية الذهبية وبنسبة 45% من العزلات الماخوذة من حالات مختلفة لاصابة الجلد , وكان معدل العزل العام للعنقوديات الذهبية من القدم المصابة بالقرح لدى مرضى السكري 5 عزلات بنسبة 33% نسبة العزلات المقاومة للمثيسيلين 20% (عزلة واحدة ),اما المصابين بخراج الجلد والدمامل والقوباء الجلدي فكان معدل عزلها كالاتي (69)18 %,(55)21% , (31)9%,نسبة العزلات المقاومة للمثيسيلين (55.6)10% , (61.9)13%, (11.11)1% على التوالي . ان معدل عزل هذه البكتيريا من عدوى الجروح والحروق فكانت كالاتي (32)11%, (44)12% نسبة العزلات المقاومة للمثيسيلين منها (72.7)8%% (16.6)2, على التوالي . ان العزلات البكتيرية من المكورات العنقودية الذهبية المكتسبة مجتمعيا مقاومة لجميع مضادات البيتالاكتام وغير مقاومة للمضادات الحيوية التي لا تنتمي الى هذه المجموعة. اجري الكشف الوراثي باستعمال تقنية سلسلة تفاعلات البلمرة polymerase chain reaction اذ شخصت العزلات الخمسة والثلاثين باستعمال 3 جينات وهم mec Agene وهو الجين الخاص بالمقاومة للمثيسيلين , وكان عدد العزلات الحاملة لهذا الجين 22 عزلة من اصل 35 عزلة وبنسبة 62% . واخضعت العزلات المقاومة للمثيسيلين الى عملية تعريف للقطعة الكروموسومية النوع الرابعSCCmec IV اذ يعد هذا النوع سمة تفريقية للعزلات البكتيرية من المكورات العنقودية الذهبية المقاومة للمثيسيلين والمكتسبة في المجتمع ، اذ وجدت بنسبة 100 % . كذلك تم التحري عن امكانية تواجد مورثة الذيفان الخارجي Panton Valatine leukocidine ( pvl ) باستعمال تقنية تفاعلات البلمرة PCR اذ شخص في 19 عزلة بكتيرية بنسبة 54% ، اقترن وجود السم بالاخص مع حالات الاصابة بالدمامل والخراج والجروح . اجريت الفحوصات المناعية عند المصابين بالتهاب انسجة الجلد , وذلك من خلال تقييم مستوى بعض الحركيات الخلوية مثل IL - 8 وLTB - 4 باستعمال طريقة الممتز المناعي المرتبط بالانزيم Enzyme linked Immune Sorbant assay وقد لوحظ زيادة معنوية في تراكيزها في مصول المرضى مقارنة بعامل السيطرة . | Community acquired methicillin resistant Staphylococcus aureus is one of the pathogenic micro - organism that cause a wide spectrum of infections in different sites of the human body where their pathogenicity is related to skin and soft tissue infection. This study is aimed to explore the role of this bacteria in different cases of skin infections.The genetic structure of these isolated strains of Staphylococcus aureus was also one of this study targets to evaluate immunological response by measurement of concentration of some cytokines as indicator of immune response. One hundred sixty nine patients with different cases of skin infection were enrolled in this study by taking one skin swab from each patient with different skin infection as Furuncle, boil, wound infection, impetigo and burns infection. All these cases were taken from out - patient clinics and private clinic along 5 months period from October 2014 to march 2015 in AL - Najaf Province , From the 169 swab samples only 76 swabs were shown to be positive Staphylococcus aureus with percent 45%.The percent of isolates of S. aureus from different cases of skin infection (5) isolates from diabetic foot with 20% of them are Methicillin resistant (one isolate) and skin abscess, boil, and impetigo were 18(69%),21(55%),9(31%)from which methicillin resistant were 10(55.6%), 13(61%) and 1(11.11%) respectively Percent of isolates from wound &burns infection were 11(32%) and 12(44%) and methicillin resistant isolates were 8 (72.7%) and 2(16.6%) respectively . The process of diagnosis of methicillin - resistant isolates was performed by using sensitivity test for cifoxitin following (CLSI,2014) instruction . Only 35 isolate showed resistant to methicillin when Cefoxitin disk was used then antibiotic sensitive test for other antibiotic disks done., Isolates of CA - MRSA showed resistant to all β - Lactam antibiotics and also to other drugs that are not belonged to this type of antibiotics. Genetic study was evaluated by using PCR technique to diagnosis the following genes in 35 isolates : mecA gene : which is considered responsible for methicillin resistant ,it was found that only 22 from 35 isolates (62%)carrying the gene. SCCmec IV gene which restricted only to Community acquired isolates of Staphylococci were explored in the isolates which considered a diagnostic character for Community acquired Staphylococci and this gene was found in 100% of isolates . Exotoxin panton - valatine toxin also diagnosed in 19 isolates with percent 54% in isolates of S. aureus using PCR technique. This exotoxin was produced in cases of Furuncle, skin abscess, and wound infection . Immunological test was introduced in this study to evaluate the innate immune response by measuring the concentration of Cytokines IL - 8&LTB4 which showed significant elevation in their concentration in comparison with healthy persons.

دراسة لاعتلال الاعصاب المحيطية عند مرضى الداء السكري من النوع الثاني == Study of peripheral neuropathy in patients with diabetes mellitus type II

Author name: اسراء ماجد نعمة عبيد العيكاشي
Supervisor name: سحر محمود جواد الجمالي | عمار جبار حمد الشمري
General topic: Biology
Specific topic: Zoology
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: Diabetes mellitus is one of the most chronic diseases that is widely spread all over the world and affects all age groups. The disease has a vast potential to affect almost every body organ, especially the peripheral nerves, which causes high mortality rates from disease complications. These complications cause damage in the organs of the body including the nerves located in the limbs that generate an intense heat resulting from the destruction of nerve fibers in addition to the acute pain resulting from the effect of diabetes in those nerves. This study was designed to determine the sensitivity of those nerves by displaying the results of nerve conduction study for patients and compared it with their clinical examination to determine the severity of numbness and assess the level of pain in the upper and lower limbs.The study was conducted at the Diabetes and Endocrinology Center in Al - Sader Medical City in Al - Najaf governorate and it included following up the case of (78) patients with diabetic peripheral neuropathy by nerve conduction study. The study was began from 5/9/2014 until 7/4/2015. It included diabetic men and women, and they were divided into three age groups (35 - 68), and the study excluded the patients with peripheral neuropathy but have foot ulceration because the nerve connect system (SUDOSCAN REPORT ) was used in the current study, cannot perform nerve conduction study for patients with foot ulceration also smoking and the patients who taking treatment for diabetic peripheral neuropathy .During this study the clinical examination for patients was conducted and compared it with the nerve conduction study of them , also recording the information were related with the history of patients ,as while asmeasuring the concentrations of urea and creatinine in serum, in addition the study was recognized the effect of diabetes mellitus on the kidneys by estimating the ratio of albumin to creatinine in the urine, or named (ACR). the study compared the glycosylated hemoglobin levels and body mass index with the nerve conduction study results for all patients.The present study has shown a statistical significant difference (P <0.05) between the clinical tests of the patients who were tested by tuning fork as while as nerves test needle test and between nerve conduction study for both sexes also at all age groups included in the study. The results of the study did not show any a statistical significant (P>0.05). difference when compared the results of nerve conduction study among the different age groups for males. For females however, there was a statistical significant difference (P<0.05) amongst the different age groups.The results of the current study did not show a statistical significant difference (P>0.05) as regards the duration of injury for males, while it revealed a statistical significant difference among females (P <0.05), the results of the current study also observed the presence of a statistical significant increase (P<0.05) in the ratio of albumin to creatinine in patients with diabetic peripheral nervous for males and females.When compared the glycosylated hemoglobin (HbA1c) levels over the past three months, there are no a statistical significant differences (P>0.05) for males and females ,while the study revealed no a statistical significant increase (P> 0.05) in the concentrations of urea in males when compared to their nerve conduction study results, as well as theconcentrations of creatinine which did not show a statistical significant difference( P> 0.05) at them.The study was also revealed the presence of a statistical significant difference (P<0.05) in the concentrations of urea in females with diabetic peripheral neuropathic and did not show a statistical significant difference (P> 0.05) in the concentration of creatinine at them. The results of the current study did not show a statistical significant difference (P>0.05) between the body mass index among males and it showed a statistical significant difference among females (P <0.05)with peripheral diabetic neuropathy.The study reached that diabetes, with long periods of infection, has a crucial role in showing and the advancement of the disease for most of the patients, particularly those who lack the suitable amount of control over the percentage of the Glucose in the blood. The study also deducted that the disease is accompanied with kidney failure for most of the studied patients. It was also revealed that the nominees of some of the patients which appeared during their examination via tuning fork as while as nerves test needle was not the cause of their infection with diabetes, therefore, the study recommended the adoption of the results of the diabetic peripheral neuropathy to diagnose and asses the infection with peripheral nervous surround diabetes.

دراسة بعض العوامل البيئية المؤثرة في بعض انواع القشريات في نهر الكوفة == Study Som of Enviromental factors offecting Some Crustacea species in the Kufa river

Author name: ايمان حساني شباط السلامي
Supervisor name: جاسم حميد رحمة الخزاعي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: اجريت هذه الدراسة للتعرف على انواع القشريات وانتشارها في نهر الكوفة , وتاثير بعض العوامل البيئية على تواجدها , اذ جمعت عينات الدراسة شهريا للمدة من اب 2014 لغاية تموز 2015 من ثلاث محطات , الاولى : تقع شمال جسر الامام علي (عليه السلام) والثانية قرب مبزل ميسان والمحطة الثالثة قرب جسر الكوفة القديم ( قريبة من دار ضيافة المحافظة ) , اذ قيست العوامل البيئية الفيزياوية والكيمياوية مثل درجة الحرارة والاس الهيدروجيني والاوكسجين المذاب والمتطلب الحيوي للاوكسجين والتوصيلية الكهربائية والكدرة , والعسرة الكلية وايون الكالسيوم . تراوحت الحدود الدنيا والعليا لحرارة الهواء 14.3) - 42.6 ) مo وحرارة الماء (13 - 30.4) مo والاس الهيدروجيني 6.8) - 9.6 ) والاوكسجين الذائب 2.9) - 9.1 ) ملغم / لتر والمتطلب الحيوي للاوكسجين 0.7) - 4.7 ) ملغم / لتر والتوصيلية الكهربائية 1162) - 2055 ) مايكروسيمنز/ سم والملوحة 0.7) - 1.31 ) جزء بالالف والكدرة 1.4) - 17.5) نفثالين وحدة كدرة والعسرة الكلية 210) - 930 ) ملغم / لتر وايون الكالسيوم 119 ) - 380 ) ملغم / لتر . تم خلال الدراسة الحالية تسجيل جديد لنوعين من القشريات الكبيرة لاول مرة في نهر الكوفة وهما الروبيان النهري Macrobrachium nipponense والسرطان النهري Potamon magnum اللذان يعودان الى رتبة عشارية الاقدام Decapoda , اذ سجل اعلى كثافة للروبيان النهري 63.58 ) ) فرد / م 3 في المحطة الاولى في شهر تشرين الاول واختفى في محطات الدراسة جميعها في شهر كانون الثاني وشباط , بينما سجل اعلى كثافة للسرطان النهري 32.89) ) فرد / م 3 في المحطة الاولى في شهر نيسان . وتم تشخيص 32 وحدة تصنيفية تعود الى رتبتين من القشريات الصغيرة , وهي رتبة Cladocera ورتبة Copepoda اذ وجد ان رتبة Cladocera هي الاكثر وفرة وغزارة في محطات الدراسة اذ تضم خمسة وعشرون نوعا , بينمراتبة Copepoda تضم سبعة انواع.تم تشخيص ثمانية اجناس من رتبة متفرعة اللوامس Cladocera وهي Bosmina , Daphnia , Ceriodaphnia , Simocephalus , Alona , Chydorus , Macrothrix وMonia , في جميع محطات الدراسة , ومن الاجناس التي سجلت اعلى كثافة هي جنس Bosmina وDaphnia بينما سجل الجنسين Macrothrix وMonia اقل كثافة اذ اختفت في معظم اشهر الدراسة وفي جميع محطات الدراسة. وتم تشخيص اربعة اجناس من رتبة مجذافية الاقدام Copepoda وهي Cyclops , Diaptomus , Microcyclops , Macrocyclops , ومن الاجناس التي سجلت اعلى كثافة هو جنس Cyclops ومن الاجناس التي سجلت اقل كثافة هو جنس Diaptomus . وتم تسجيل جديد لاحدى عشر نوع من القشريات الصغيرة Microcrustacea لاول مرة في نهر الكوفة تعود لرتبتين هما Cladocera وCopepoda وهذه الانواع هي umholtzil Daphnia , Daphnia galeata , Daphnia magna , Daphnia retrocurva , Simocephalus sexpinosus , Alona affinis , Chydorus gibbus , Monia micrura , Cyclops strenuous , Macrocyclops albidus , Microcyclops sp . , اظهرت نتائج التحليل الاحصائي وجود علاقة ارتباط بين كثافة القشريات والعوامل الفيزياوية والكيمياوية المقاسة شهريا ولمدة عام , كذلك لوحظ وجود فروق معنوية بين اشهر ومحطات الدراسة بالنسبة لكثافة القشريات والعوامل الفيزياوية والكيمياوية عند مستوى P ≤ 0.05 . | This study was conducted to identify the types of Crustaceans and distribution in Al - Kufa River and the effect of some environmental factors on their presence , Study samples were collected monthly for the period from August 2014 to July 2015 of three stations, the first station is located north bridge Imam Ali , the second near the Missan trocar and the third station near old Kufa bridge . The Environmental factors, were measured ( physical and chemical) such as temperature , pH , dissolved oxygen, , electrical conductivity, salinity , turbidity ,total hardness (T . H) and calcium ion. Lower and upper limits for air temperature ranged from (14.3 - 42.6) Co and water temperature (13 - 30.4) C0 , pH (6.8 - 9.6) , dissolved oxygen (2.9 - 9.1) mg / L , the biological oxygen demand (BOD5) (0.7 - 4.7) mg / L), electrical conductivity (1162 - 2055) µs/ cm and salinity (0.7 - 1.31) ppt , turbidity (1.4 - 17.5) NTU, total hardness (210 - 930) mg / L and calcium ion ( 119 - 380) mg / L. During the current study, a new record of two species of Macrocrustaceans for the first time in AL - Kufa River Macrobrachium nipponense and river crab Potamon magnum which they return to the Order Decapoda, The highest density was recorded for the M. nipponense (63.58) individual / m 3 in the first station in October month and disappeared in the study, all stations at January and February, months , while the highest density of river crab P. magnum record (32.89) individual / m 3 in the first station in April month. in this study diagnosed 32 taxonomic unit return to Microcrustacea : Cladocera and Copepoda orders , also cladocera reverded where found that the most abundant and the abundance of plants in the study are Cladocera rank as comprising twenty - five type, while Copepoda rank of seven . Diagnosed eight genus belong to the Cladocera such as Bosmina, Daphnia, Ceriodaphnia, Simocephalus, Alona, Chydorus, Macrothrix, Monia, in all study stations, The genera Bosmina and Daphnia recorded high density while Macrothrix and Monia recorded low density which disappeared in the most study month in all study stations. Also four genera of the Copepoda were diagnose such as Cyclops, Diaptomus, Microcyclops and Macrocyclops, and genus Cyclops record high density while the genus Diaptomus recorded low density. eleven species of Microcrustacea were recorded for the first time in AL - Kufa River belonging to the Cladocera and Copepoda orders and these species are Daphnia umholtzil, Daphnia galeata, Daphnia magna, Daphnia retrocurva, Simocephalus sexpinosus, Alona affinis, Chydorus gibbus, Monia micrura , Cyclops strenuous, Macrocyclops albidus, and Microcyclops sp. Statistical analysis showed a correlation relationship between the density of Crustaceans and the physical and chemical factors measured per month for one year, also noted that there were significant differences between months and study stations for the intensity of Crustaceans and the physical and chemical factors at P ≤ 0.05

دراسة تاثـير المستخلصات المائيـة لنباتي الليمـون الحامـض والسعـد في ذكور الجرذان البيض السليمة والمصابة تجريبيا بداء السكري == Studying the Effect of Aqueous Extract of Citrus limon and Cyperus rotandus in alloxan induced in Diabetic Male Albino Rats

Author name: زهراء كريـم عبد الله حسن الميالي
Supervisor name: زينب شنيور مهـدي الطرفـي
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: داء السكري هو اضطراب ايضي مزمن يتصف بارتفاع سكر الدم مع اضطراب في ايض الكاربوهيدرات والدهون والبروتين نتيجة لخلل في افراز الانسولين من قبل البنكرياس ، او تاثيره الحيوي اوكليهما، يشكل هذا الداء تهديد خطير ومتزايد على حياة الكثير من الناس ويعد المشكلة الرئيسية التي تودي بحياة الملايين، وما للنباتات الطبية اثر مهم في صحة الانسان كونها مصدرا مهما للعديد من الادوية والمستحضرات الطبية , وتكمن الفائدة الطبية لهذه النباتات في قابليتها على انتاج العديد من المركبات الكيميائية ذات الخصائص الصيدلانية التي تدخل كمادة اولية او عوامل مساعدة في صناعة الادوية ولكثرة استخدام الاعشاب (النباتات الطبية) في الوقت الراهن بوصفها الطب البديل جاءت هذه الدراسة . اجريت هذه الدراسة الحالية في كلية التربية للبنات / جامعة الكوفة للمدة من 2014/10/1 م ولغاية 2015/5/30 م وتضمنت الدراسة تحضير المستخلص المائي الحارلكلا النباتين (بذور الليمون الحامض Citrus limon ودرنات السعد (Cyperus rotundusوقد تضمنت الدراسة متابعة (42) حيوان من ذكور الجرذان البيض ، وتم تقسيمها الى سبعة مجاميع تضمنت كل مجموعة ستة ذكور ، اذ جرعت المجموعة الاولى فمويا بمحلول الملحي الفسيولوجي حسب وزن الجسم واعتبرت كمجموعة سيطرة (سالبة) ، اما المجموعة الثانية فقد حقنت تحت الجلد بالالوكسان (1 مل) واعتبرت كمجموعة سيطرة مصابة (موجبة)، اما المجموعة الثالثة فقد جرعت فمويا بالمستخلص المائي الحار لبذور الليمون الحامض بتركيز (500ملغم/كغم) بتركيز (750ملغم/كغم) وحسب وزن الجسم ، والمجموعة الرابعة جرعت فمويا بالمستخلص المائي الحار لدرنات السعد حسب وزن الجسم ، والمجوعة الخامسة فحقنت بالالوكسان تحت الجلد ومن ثم جرعت فمويا المستخلص المائي الحار لبذور الليمون الحامض بتركيز (500ملغم/كغم) وحسب وزن الجسم، والمجموعة السادسة التي حقنت بالالوكسان تحت الجلد ومن ثم تم تجريعها فمويا المستخلص المائي الحار لدرنات السعد بتركيز (750ملغم/كغم) حسب وزن الجسم ، واخيرا المجموعة السابعة التي حقنت بالالوكسان تحت الجلد ومن ثم جرعت فمويا بعقار الاميرال (Glimepiride) بتركيز (0.1) ، علما ان التجريع قد استمر لمدة ثلاثين يوما كاملا وبواقع مرة واحدة يوميا .وقد اظهرت نتائج الدراسة ما يلي :  حصول انخفاض معنوي (P< 0.05) في وزن الجسم واوزان الاعضاء (البنكرياس والكبد والكلية) لدى مجموعة السيطرة الموجبة(المحقونة بالالوكسان) عند مقارنتها بالمجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي، وعلى النقيض من ذلك فقد اظهر وزن الجسم وكذلك اوزان الاعضاء ارتفاعا معنويا (P< 0.05) لدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة.  وجود انخفاض معنوي (P< 0.05) في التعداد الكلي لخلايا الدم البيض وكريات الدم الحمر وتركيز الهيموغلوبين والصفيحات الدموية عند الحيوانات المصابة تجريبيا بداء السكري عند مقارنتها مع المجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي ، على العكس من ذلك حصول ارتفاع معنوي (P< 0.05) في مجموعة الحيوانات السليمة لدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة . حصول ارتفاع معنوي (P< 0.05) في مستويات الكلوكوز ، الكولسترول الكلي الكليسيريدات الثلاثية ، والبروتينات الدهنية واطئة الكثافة والواطئة الكثافة جدا وحصول انخفاض معنوي (P< 0.05) في مستوى البروتينات الدهنية عالية الكثافة لدى مجموعة السيطرة الموجبة عند مقارنتها مع المجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي ، وكذلك حصول انخفاض معنوي (P< 0.05) في المعايير المذكورة اعلاه وارتفاع في البروتينات الدهنية عالية الكثافة في مجموعة الحيوانات السليمة ولدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة . وحصول ارتفاع معنوي (P< 0.05) في فعالية الانزيمات الكبدية ALT, AST وALp والبلروبين لدى مجموعة السيطرة الموجبة عند مقارنتها مع المجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي ، وحصول انخفاض معنوي (P< 0.05) في فعالية الانزيمات اعلاه في مجموعة الحيوانات السليمة ولدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة . حصول انخفاض معنوي (P< 0.05)في مستوى البروتين الكلي والالبومين والكلوبيولين لدى مجموعة السيطرة الموجبة عند مقارنتها مع المجاميع : السيطرة السالبة والمجموعتين المعاملتين بالمستخلص المائي الحار لبذور الليمون الحامض ودرنات السعد على التوالي والمجموعة المعاملة بالالوكسان وعقار الاميرال وكذلك المجموعتين المعاملتين بالالوكسان ومستخلص النباتين المذكورين على التوالي ، وحصول ارتفاع معنوي(P< 0.05) في في مجموعة الحيوانات السليمة ولدى المجموعتين المصابتين تجريبيا بداء السكري والمعاملتين بمستخلص النباتين كلا على حدا عندما تقارن بمجموعة السيطرة الموجبة . اشارت نتائج الدراسة النسجية للكبد حصول تغير نسجي للكبد في حالة ارتفاع السكر بالدم تمثل بحصول توسع بسيط بالجيبانيات ،واحتقان دموي مع حدوث تنخر للخلايا الكبدية ونزف فيها ، وظهور مناطق التهابية عدة واستسقاء مائي وبعد معاملة المجموعة المصابة بالمستخلص المائي لكلا النباتين لوحظ رجوع الشكل الطبيعي للخلايا الكبدية . اشارت نتائج الدارسة النسجية للكلية حصول تغير نسيجي فيها في حالة ارتفاع الكلوكوز بالدم والمتمثل بخروج الانوية من الكبيبة الكلوية وتجمعها في اماكن متفرقة خارج الكبيبة وكذلك وجود نزف وتحطم في جدران الكبيبة والاوعية الدموية الموجودة ضمنها وتحطم واضح للقشرة الكلوية وعند معالجة الحيوانات المصابة بالمستخلص المائي لكلا النباتين لوحظ رجوع الشكل الطبيعي للكلية . اشارت نتائج الدارسة النسجية للبنكرياس حصول تغير في التركيب النسيجي للبنكرياس في حالة الاصابة بالسكري نتيجة الحقن بمادة الالوكسان تتمثل بوجود النزف الحاد في جميع مناطقها وحصول توسع وتحطم في الخلايا وعدم انتظام اشكالها وانحلال الانوية وخروجها من اماكنها الصحيحة ولكن عند معالجة الحيوانات المصابة بالمستخلص المائي لكلا النباتين لوحظ رجوع الشكل الطبيعي للبنكرياس.وقد سجلت النتائج جميعا بشكل عام تاثيرا افضل للمستخلص النباتي لبذور الليمون الحامض قياسا بدرنات السعد والدرنات افضل من العقار في تخفيض سكر الدم اضافة الى تحسين الاضطرابات الايضية المتسببة عن داء السكري المستحدث بعقار الالوكسان . | Diabetes Mellitus is a metabolic disorder characterized by hyperglycemia due to a defect in Insulin secretion from the pancreas, insulin action or both, with disturbances in (carbohydrates, lipid and protein) metabolic due to a defect in Insulin secretion, Insulin action or both. This disease is a serious and growing threat to the lives of many people , it isthe main problem which cause death to millions,The medical plants have important impact on human health as an important source of many medicines and medical products. The medical advantage of these plants lies in their ability to produce many of the characteristics that the pharmaceutical intervention have as a primary material or contributing factors in the pharmaceutical industry chemical compounds. The frequent use of herbs (medical plants) at the present time as alternative medicine, as came in this study.This study was conducted at the Faculty of Education /University of Kufa during the period from2/10/ 2014 to 30/5/ 2015. The study included the preparation of aqueous extractsofCitrus lemonseeds and Cyperus rotundus tuber. Also the study includedthe use ofaqueous extractsofCitrus lemon at concentration (500) mg/kg andCyperus rotundus at concentration (750) mg/kgineach dosageaccording to the body weight of the albino rats (Ratus ratus). The study includes (42) white malerats divided into seven groups with six rat in each group and they were injected according to their weight in the following way : the first group was orally dosed with physiological saline and it was considered as a control(negative), the second group was injected with alloxan (1ml.) and were considered as infected control group (positive). The third group were also dosed orally with decocted extract of citrus lemon seeds in (500mg/kg). The fourth group was dosed orally with decocted extract of Cyperus rotundus (750mg/kg) concentration. The fifth group was first hypodermically injected with alloxan and then orally dosed with decocted extract of citrus lemon seeds in (500mg/kg) concentration. The sixth group was first hypodermically injected with alloxan and then orally dosed with decocted extract of Cyperus rotundus (750mg/kg) concentration. The seventh group was first hypodermically injected with alloxan and then orally dosed with Glimepiride with (0.1) concentration. The dosing continued for 30 days.  A significant decrease (P< 0.05) was shown in total body weight and organ weight such as pancreas, liver and kidney in uninfected control(Negative) when compared with other groups : in the two groups that were treated with hot aqueous extract of Citrus lemon seeds ad Cyperusrotundus tuber respectively, also,the group was treated with Amyral and the group that injected with alloxan and that treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, we alsofound a significant increase (P< 0.05) in the above criteria in the two groups that were injected by alloxan and treated by two extracts when compared with the positive control group.  A significant decrease (P< 0.05) was shown in the total WBC and RBC count , and the hemoglobin concentration platelets in uninfected control group when compared with other groups : in the two groups that were treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, respectively, also the group was treat with Amyral and the group that were injected with alloxan and that which was treated with hot aqueous extracts of Citrus lemon seeds and Cyperusrotundus tuber, also a significant increase (P< 0.05) was shown in the above criteriain diabeticanimalsgroup compared with healthy control group, we also found a significant increase (P< 0.05) was shown in the two groups that were injected by alloxan and treated by two extracts when compared with positive control group.  A significant increase (P< 0.05) was clear in the levels of glucose , total cholesterol, triglyceride, low density lipoprotein , very low density lipoprotein and a significant decrease (P< 0.05) in high density lipoprotein in uninfected control group when compared with other groups : in the two groups that treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, respectively.Also the group was treated with Amyral and the group that injected with alloxan and that treated with hot aqueous extract of Citrus lemon seeds ad Cyperusrotundus tuber, a significant decrease (P< 0.05) was clear in the above criteria and significant increasein high density lipoprotein in diabeticanimalsshown in the two groups that were injected by alloxan and treated by two extracts when compared with the positive control group.  A significant increase (P< 0.05) the effectiveness of hepatic enzymes(ALT , AST ,ALP, Bilirubin) in uninfected control group when compared with other groups : in the two groups that were treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, respectively, also the group was treated with Amyral and the group that injected with alloxan and that treated with hot aqueous extract of Citrus lemon seeds and Cyperusrotundus tuber, also a significant decrease (P< 0.05) in the effectiveness of hepatic enzymes in diabeticanimals, a significant decrease (P< 0.05). The effectiveness of the above enzymes in the two groups that were injected by alloxan and treated by two extract when compared with the positive control group.  The presented histological study of the liver refers to the histological changes in the liver in the state of hyperglycemia that is represented by the expansion of sinusoid, blood accumulation, necrosis and bleeding in hepatocyte, presence of many inflammation areas, watery dropsically but when treating diabetic animals by aqueous extract of (Citrus lemon andCyperusrotundus), it wasnoted thatthey returned back to the normal shape of hepatocyte. The histological study of the kidneyrefers to the occurring of the histological changes in the kidney in the state of hyperglycemia that were represented by getting out the nucleus from glomerulous and aggregation in many area out theglomeruli, also, the bleeding or the destruction of glomerulous wall with blood vessels, the present distraction in kidney cortex but when treating diabetic animals with aqueous extract of (Citrus lemon and Cyperusrotundus), it was noted that it returned back to the normal shape of kidney. The histological study of the pancreasrefers to the occurring of the histological changes in infected diabetic animals by (alloxan) which represented an acute bleeding in all regions, destruction and expansion in kidney cells, abnormal shape, nucleus degenerative and leaving the true area but when treating diabetic animals with aqueous extract of (Citrus lemon and Cyperusrotundus), it was noted that it returned back to the normal shape ofpancreas.In conclusion, the results of this study indicate that the Citrus limonseeds and Cyperus rotundus tuberextracts gave good results, even better than the reference drug Amyral for loweringbloodsugar, in addition to improvingmetabolic disorderscaused byalloxan induced-diabetes

دراسة لتكوين الغشاء الحيوي لبكتريا Escherichia coli وProteus mirabilis المعزلة من اصابات المسالك البولية في محافظة النجف الاشرف == Study of Biofilm formation of Escherichia coli and Proteus mirabilis isolated from urinary tract infection in AL - Najaf Al - Ashraf governorate

Author name: رسل عيدان محسن
Supervisor name: سهام جاسم محسن
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Najaf
First pages:
Abstract: The aim of this study is to inferring the ability of biofilm production and virulence factors which has helped to form it into two types of bacteria P.mirabilis and E.coli. Two hundred and fifty samples have been collected from patients who are suffering Urinary tract infection (UTI) in Teaching Hospital at Al - Najaf Al - Ashraf during the period ( 1/10/2014 - 1/1/ 2015). The samples have has identified by diagnostic methods : culturing , microscopic , biochemical and using VITEK - 2 compact system more sensitively and specifically method.The results have been showed that the isolation ratio was 30 (37.5%) of E.coli, while P.mirabilis was 14(17.5%) also, a five isolates (6.5%) wereEnterobacter cloacae ssp cloacae and only two isolates (2.5%) were Raoultella ornithinolytica from the total number of specimens. The virulence factors and antibiotic sensitivity test of two bacteria : P.mirabilis and E.coli were determined and the more virulence and multidrug resistance were selected.The hemagglutination properties of P.mirabilis which isolated from clinical samples were determined , the reaction with red blood cells of human group O+ was explored. The ratio of Hemagglutination was reached 42.86%, 50%, 7.14% to MSHA, MRHA and NOHA respectively. However, E.coli has been a different results that was reached 30 % , 26.67% and 43.33% from type MSHA, MRHA and NOHA as well. On the other hand, the thin - film production (pellicle) has been formed by 13 isolates of bacteria P.mirabilis and 21 isolates of bacteria E.coli, when it was growth on the nutrient broth in a temperature 37˚C for 24 - 18 hours. Moreover, it has been shown that there is high affinity for adhesion with the epithelium cell in the urinary tract. In addition, the E.coli has proved the higher efficiency than P.mirabilis in adhesion. It was detected ability of bacteria to biofilm production in three methods. The bacteria E.coli have showed their ability on biofilm production where by the rate was (43.3, 52 and60)% on the different growth ways which was the Congo Red, the tube and the plate respectively.The gel electrophoresis has been done on all isolates which was to analyzed on adhesive factors genetically whereby the bacteria P.mirabilis, E.coli have shown ability to gene expression (fimH and afaB) with a rate of (42.86 and 28.57)% and (30 and 20)%, respectively.Also, it has been clarified that the antibiotics ( Ciprofloxacin, Amikacin and Ceftazidime) have an effect on the most virulent bacteria to prevent the formation of biofilm when it is taken a half of minimum inhibitory concentrations. It was found that the minimum inhibitory concentrations were the most efficient in the prevent a biofilm formation in both types of bacteria.It appears to be found that the minimum inhibitory concentrations of these antibiotics were the most efficient in the prevent the formation of biofilm in both types of bacteria. Ciprofloxacin has the high efficiency to prevent the biofilm formation in E.coli while this concentration has no effective on biofilm production from others. It has been found that there is a clear positive relationship between the presence of adhesion gene (fimH , afaB) and the ability to adhesion on the epithelial .The conjugation process was conducted successfully to transfer the adhesion genes between (E.coli and P.mirabilis) which have been considered a donor strains to transfer the genes to the standard strain E.coli HB101 which have been considered a receipt strains whereby have shown ability to adhesion and biofilm formation.

دراسة علاقة مجاميع الدم ABO مع البين ابيضاضي 18 المرتبط بالبروتين الفا والبين ابيضاضي 18 مستقبل البيتا في النساء العراقيات العربيات المصابات بالسكري من النوع الثاني == Studying the Association of ABO Blood Groups with IL - 18BPa & IL - 18R beta in the Iraqi Arab Females with Diabetes Mellitus Type 2

Author name: زينب فاضل عبد الغفور
Supervisor name: اسماء محمد صالح المهيدي
General topic: Biology
Specific topic: zoology - Genetics
Degree: Master
Language: English
University location: Baghdad
First pages:
Abstract: داء السكري كمرض التهابي يرتبط مع العديد من العوامل الالتهابية لذلك تهدف الدراسة الحالية لحساب تركيز العامل البين ابيضاضي 18 المرتبط بالبروتين الفا والعامل البين ابيضاضي 18 مستقبل البيتا مع عوامل الخطورة التقليدية والتي تشمل المقاييس الفيزيائية مثل متوسط محيط الخصر ومؤشر كتله الجسم وعوامل البيو كيميائية مثل الانسولين , مقاومة الانسولين , مستوى السكر في الدم في حاله الصيام ,مستوى الهيموغلوبين السكري وملف الدهون وفي الوقت نفسه دراسة ارتباطهم مع نظام الدم ABO في النساء العراقيات العربيات المصابات بالسكري من النوع الثاني ومن اجل معرفة تاثير ذلك مع عوامل اخرى التي تعتبر كمؤشر او مطور لمرض السكري من النوع الثاني . وشملت الدراسة الحالية 340 مريضا من النساء العراقيات العربيات المشخصات حديثا بالسكري من النوع الثاني 200 من النساء الاصحاء اختبرت للتكرار الاليلي والمظهري ,ومن هؤلاء 60 مريضة اختبرت للدراسة المناعية والكيميائية مقارنة مع 28 من النساء الاصحاء .الاختبارات المناعية بواسطة استخدام تقنية الانزيم المرتبط المناعي, اما المقاييس الكيميائية باستخدام الاساليب الانزيمية واللونية, وتم تقسيم المرضى والسيطرة حسب مجاميع الدم الى اربعة مجاميع ثانوية (A , B ,AB ,O) وفقا لنوع فصائل الدم التي كشف عنها بواسطة الاختبارات المصلية القياسية .اظهرت البيانات الحالية : 1 - زيادة معنوية في المصل العامل البين ابيضاضي 18 المرتبط بالبروتين الفا في المرضى الحاليين ,في حين لم تظهر اختلافات معنوية في مستوى مصل العامل البين ابيضاضي 18 مستقبل البيتا .2 - زيادة معنوية في متوسط العامل البين ابيضاضي 18 المرتبط بالبروتين الفا مع زيادة العمر في كل من المرضى ومجموعه السيطرة, في حين العامل البين ابيضاضي 18 مستقبل البيتزااد في المجموعة الاقل من 40 سنة وانخفض مع زيادة العمر في كل من المرضى ومجموعه السيطرة .بالاظافة الى زيادة كل عوامل الخطورةالاخرى.3 - تركيز كلا العاملين العامل البين ابيضاضي 18 المرتبط بالبروتين الفا والعامل البين ابيضاضي 18 مستقبل البيتا يزداد بشكل غير معنوي تدريجيا في كل المرضى مع زيادة مدة المرض ونفس النتيجة لمقاومة الانسولين.4 - اظهرت نسبة الهيموغلوبين السكري اقل من 7.3 % زيادة كبيرة في متوسط العامل البين ابيضاضي 18 المرتبط بالبروتين الفا , العامل البين ابيضاضي 18 مستقبل البيتا وتنخفض تدريجيا مع زيادة نسبة الهيموغلوبين السكري .5 - الافراد الذين كانوا يحملون فصيلة الدم B اظهروا اعلى معدل لمتوسط العامل البين ابيضاضي 18 المرتبط بالبروتين الفا , الانسولين ومقاومة الانسولين .6 - المرضى الذين كانوا يحملون فصيلة الدم B والاصحاء الذين يحملون فصيلة الدم A اظهروا اعلى معدل لمتوسط العامل البين ابيضاضي 18 مستقبل البيتا .7 - يعتبر العامل البين ابيضاضي 18 المرتبط بالبروتين الفا والعامل البين ابيضاضي 18 مستقبل البيتا كعوامل خطورة مستقلة في النساء العراقيات العربيات المصابات بالسكري من النوع الثاني والتي تدعمها غياب العلاقة المعنوية بين العامل البين ابيضاضي 18 المرتبط بالبروتين الفا والعامل البين ابيضاضي 18 مستقبل البيتا وجميع عوامل الخطورة التقليدية الاخرى .8 - هنالك علاقة ايجابية بين مستويات العامل البين ابيضاضي 18 المرتبط بالبروتين الفا وخطورة السكري من النوع الثاني والذي قد يعتبر كمؤشر لحدوث المرض , في حين ان مستويات العامل البين ابيضاضي 18 مستقبل البيتا قد يكون مؤشر لتطور المرض .مزيد من الدراسات اللازمة لفهم العلاقة بين مجاميع الدم , عوامل الالتهابية ومخاطر السكري من النوع الثاني . | DM is considered as inflammatory disease associated with many inflammatory factors. As such the present study aims to investigate the concentration of IL - 18 binding protein alpha (IL - 18BPa) and IL - 18R beta with classical risk factors which include Anthropometric risk factors like Age, Central Obesity (CO) and body mass index (BMI), biochemical factors like Insulin , Insulin Resistance (IR), Fasting Blood Glucose ( FBG), Glycosylated Hemoglobin (HbA1c) and lipid profile. Besides, the study aims to studying their association with ABO blood system in the Iraqi Arab female patients with diabetes mellitus type 2 in order to figure out their effect with other factors as predictor or developer for diabetes mellitus type 2. The present study included 340 Iraqi Arab female patients newly diagnosed with DMT2 and 200 healthy individuals tested for phenotype and allele frequency. From those, 60 patients were tested for the immunological and chemical where compared to 28 healthy individuals. Immunological tests by using ELISA kits. While Chemical parameters using enzymatic and colorimetric methods. The study groups (patients and control) were divided into four sub groups (A, B, AB and O) according to the class of blood groups which were tested by using standard serological procedures. The present data showed : 1 - A significant increase in the serum level of IL - 18BPa in the patients of the present study, while there was no significant difference in the serum level of IL - 18R beta. 2 - A significant increased in mean of IL - 18BPa with increased age in both patients and control groups. While IL - 18R beta showed increase at group less than 40 year and decreased with increasing age in both patients and control group. Besides increased all other risk factors. 3 - The levels of both IL - 18BPa and IL - 18R beta were gradually non significantly increased the level in each patients group with an increased duration of disease and the same for IR. 4 - Patients with HbA1c less than 7.3% showed a significant increase in mean of IL - 18BPa and IL - 18R beta and decreased gradually with the increase of HbA1c. 5 - Patients and control subjects who carried blood group B had the highest mean of IL - 18BPa, Insulin and Insulin resistance. 6 - Patient subjects who carried blood group B and control subjects who carried blood group A showed the highest mean of IL - 18R beta. 7 - IL - 18BPa and IL - 18R beta act as independent risk factors in Iraqi Arab females with DMT2 which supported by the absence of significant correlation among IL - 18BPa, IL - 18R Beta and all others classical risk factors. 8 - A positive relation between levels of IL - 18BPa and risk of DMT2. This may be an indication of inflammation for newly diagnostic diabetic patients, while Serum levels of IL - 18R beta might be a predictor marker of disease progression. Further studies are needed to understanding the relationship between blood groups, inflammatory markers and risk of DMT2 in healthy population.

دراسة حيوية وتشخيص جزيئي لداء الاكياس العدرية في المضائف الوسطية اعتمادا على تحليل تتابع جينات rDNA - ITS1 وmtCOX1 في محافظة ذي قار == Biological Study and Molecular Identification on Hydatidosis in Intermediate Hosts Depending on Sequence Analysis for rDNA - ITS1 and mtCOX1 Genes in Thi - Qar Province

Author name: وسام جاسم حنش
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was carried out in Thi - Qar province during the period from beginning of January until the end of December 2015 to investigate the prevalence of infection with hydatidosis in human and other intermediate hosts. The molecular techniques was used to detect the strain of Echinococcus granulosus. The present study diagnosed 58 infection with hydatidosis in different organs such as liver, lungs, kidney and spleen of surgically treated patients in AL - Hussein Imam Teaching Hospital inNassiriyah city, centre of Thi - Qar province. The total number of hydatid cysts was 72 cyst in different organs with total intensity of infection 1.24. The females recorded highest percentage of infection 67.24% than males 32.76 %, and the age group 21 - 30 year showed highest percentage of infection 24.14 % . The lowest percentage recorded in age group more than 60 year 5.17 %.The results of study showed that right lobe of liver was more affected 58.62 % than left lobe 13.79 %. The percentage of left lung infection was 10.34 % while right lung 8.62 %. The total average of fertile cysts in infected organs was 81.82 % and sterile cysts 18.18 %. The highest percentage of infection was recorded in house women 87.18 %. The number of recorded cases in Nassiriyah district was 21 case with percentage of infection 36.21 %.During the same period a total of 1303, 1287, 405, 90, and 294 head from sheep, cattle, buffaloes, camels and goats were examined respectively.The percentage of infection was 7.83 %, 7.69 %, 3.46 % and 2.22 % in sheep, cattle, buffaloes and camels respectively, and no infection was recorded in goats. The intensity of infection was 4.07, 3.27, 1.64 and 2 in sheep, cattle, buffaloes and camels respectively. The results of statistical analysis showed no significant differences (P????0.05) in percentage and intensity of infection.The percentage infection of liver, lungs and both together in sheep was 5.45 %, 1.23 % and 1.15 % respectively, and there was no significant differences in percentage of infection but intensity of infection differed significantly(P????0.05). The percentage of liver, lungs, both together and spleen infection in cattle were 3.11 %, 2.95 % , 1.55 % and 0.08 % respectively while intensity of infection in liver, lungs, both together and spleen was 2.5, 2.18, 7 and 1 respectively. The percentage of liver, lungs and both together infection in buffaloes were 2.22 %, 0.99 % and 0.25 % respectively while intensity of infection in liver, lungs and both together was 1.89, 1 and 2 respectively. No significant differences in percentage and intensity of infection in camels andboth percentage and intensity of infection in liver and lungs were 1.11% and 2 respectively.High significant differences was recorded between percentage infection in males and females of animals. The percentage of infection in females were 15.87 %, 13.42 %, 6.99 % and 3.45 % in sheep, cattle, buffaloes and camels respectively. Highest percentage of infection were 0.72 %, 0.18 % and 0.46 % in males of sheep, cattle and buffaloes respectively. No infection were recorded in males of camels.T he age was effect on percentage of infection and big aging groups recorded in sheep, cattle, buffaloes and camels. The present study recorded significant differences in percentage of fertile hydatid cysts in liver and lungs of animals and highest of percentage of fertility recorded in liver and lungs of sheep 84.21 % and 79.24 % respectively and lower percentage of fertility was in liver of buffaloes 11.11% and liver of cattle 14.70 %. The lower percentage of sterile hydatid cysts was in liver and lungs of sheep 15.79 % and 20.75 % respectively and highest percentage recorded in liver of buffaloes 88.88 % and spleen of cattle 100 %.S t a tistical analysis using of ANOVAs test showed no significant differences in total length, length of blade and width for large and small protoscolices hooks of hydatid cysts isolated from liver and lungs of sheep, human, cattle buffaloes, camels and hooks of sheep which is showed genetic variations (new strain).The PCR - RFLP technique was used in identification of E.granulosus strains infecting human and intermediate hosts in Thi - Qar province. The molecular study is carried out on 75 hydatid cysts collected from human, sheep, cattle, buffaloes and camels. The DNA is extracted from protoscolices of hydatid cysts isolated from infected organs. PCR - RFLP technique showed that sheep strain G1 of E.granulosus is predominant in Thi - Qar province depending on amplification of rDNA - ITS1 gene. The PCR products in all DNA samples showed 1000 bp approximately. Alu1 endonuclease with rDNA - ITS1 demonstrated two bands 800 bp and 200 bp approximately, while Rsa1 endonuclease showed two bands 655 bp and 345 bp approximately which is similar to sheep strain genotype 1.Sequence analysis for rDNA - ITS1 gene showed the presence of sheepstrain genotype 1 which is similar 100 % to sheep strain in gene bank exceptone isolate (sheep liver) was differs from other strains in position ofnucleotide sequence. It was similar 99 % for sequence of sheep strain. Thedata base of this new strain was recorded in gene bank for DNA sequence National Center for Biotechnology Information (NCBI) and European Nucleotide Archive (ENA) for the first time globally under the name Echinococcus granulosus strain WH1AW2 (GenBank : LT547814). Polymerase chain reaction technique and sequence analysis of mtCOX1 gene showed the presence of sheep strain G1 of human, sheep, cattle, buffaloes and camels. The sequence analysis showed the genetic variation in mtCOX1 gene for hydatid cyst in infected sheep represented by point mutation type transition which caused change Adenine to Guanine, and sequence data base of the sample recorded for first time in NCBI and ENA (GenBank : LC203589). Buffaloes strain G3 isolated from human lung recorded for first time in Thi - Qar province depending on comparison of samples sequences with source strains recorded in gene bank (NCBI) using "BLAST". The size of bands for mtCOX1 gene was 450 bp in all samples

استخلاص وتنقية وتشخيص ببتيد كلايكوسيدي من الحبار Loligo sp. ودراسة تاصيرة على خط الخلايا السرطانية Hela Cell Line والطبيعية REF == Extraction , Purification, Characterization Glycosidic Peptide From Loligo sp. and Study Effect On Cancer Cell Line (Hela) and Normal (REF)

Author name: هيفاء عدنان منصور الموسوي
Supervisor name: بلسم انيس مارينا | ضمياء قاسم سكر
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was designated to investigate the capcity of pure Loligo sp extract on two cell lines : - the cancer cell line Human cervix uteri epitheloid carcinoma (Hela) and Rat Embryo Fibroblast cell line (REF) as normal cell line.The study included extraction of tissue (whole body) of Loligo sp by using 30% ammonium sulfate. and chemical group detected in the extract by using qualitative chemical test and the result showed that the crud and pure extract contained alkloides, proteins, carbohdrates, saponines, flavonoids, aldehyde &keton group and phenol group .and the extacte does not contain glycosides.The crude extraction was purified by column chromatography using sephadex G - 25. and the purity of extract detected by using polyacryl amide gel electrophoresis under non denaturated condition. The result showed that the extracted riched with amino acid and some carbohydrate by using high performance liquid chromatography (HPLC) with compared standerd amino acid and suger.Infrared Spectroscopy of purified extract shows that it contain (N - H - C - OH) to emphasize on found amino acid in the extract.The cytotoxicity effect on cell lines study by four concentraction ( 125, 250 ,500 and 1000)ϻg/ml were prepared and tested on cell line with five replicates for each concentraction, the optical density of cell growth read by the Elisa reader 550 nm and use by tetra zolium bromide (MTT). The result for in vitro study showed that all concentraction had high inhibition on tumor cell line and a highly significant inhibition in (Hela) line was recoreded 42.79% at exposure time 48hr in the concentraction 1000 ϻg/ml the effect was dose dependent. The inhibitory effect of extract on Hela cell line higher than in the REF. while it was less effect of extract on Rat Embryo Fibroblast cell line after exposure time 48hr.

مستويات الاديبونكتين والابلين والهرمونات الاخرى والمعايير الدموية اثناء الحمل وفي مرضى متلازمة المبيض متعدد الاكياس == Adiponectin, Apelin and other Hormones Levels and Blood Indices during Pregnancy and in Patients with Polycystic Ovaries Syndrome

Author name: هناء سلمان كاظم
Supervisor name: طه جاسم الطه | علي فالح الاسدي
General topic: Biology
Specific topic: Zoology - Physiology
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study aimed to determine the levels of adiponectin and apelin and their relations with all parameters. Investigation in the present study included; hormones, lipid profile, liver enzymes activity and haematological profile in groups of Iraqi Basrah pregnant women during different months of pregnancy and to correlate these levels to maternal age, Body Mass Index, excepted parity and sex of fetuses and correlated with level of adiponectin and apelin hormones only and the results were compared with non - pregnant females as control group. The present study included (180) women ranging from 20 - 40 years old, out of them (70) pregnant and (20) non - pregnant women with polycystic ovary syndrome (PCOs), and (90) females non - pregnant were considered as control group. The pregnant women attended the obsteric units in the general state hospitals in Basra Governorate. They are as follows : Al - Basra General Hospital for Maternity and Childhood, Al - Mawane, Al - Fayha’a and Basra General Hospital which drain most patients from urban and rural areas. None of the selected women suffered from any type of disease. Required data were collected by the researcher depending on direct interview with women before being admitted to the study, using a questionnaire including information on age, parity, month of pregnancy, and geographical area..The Women were divided into two age group (≤ 27 and ≥28 years). Moreover, females were also divided according to BMI into normal (≤ 25), over weight (25 - 30) and Obese (≥30).The month of gestation was determined not only from the date of the women’s last menstruation (LMP) but also according to the ultrasonic reports that pregnant women had. Blood samples (10 ml) were taken from pregnant women at the end of each month of pregnancy and from control group as well as from women with PCOs. Each blood sample was divided into EDTA tube (2 ml) which was used for complete hematological picture study (RBC, Hb, PCV, PLT, WBCs count and WBCs differential count) and the plain tube (8 ml) where the serum was obtained, for biochemical study (Adiponectin, Apelin, testosterone, Progesterone, estradiol, follicular stimulating hormone, luteinizing hormone, insulin, lipid profile and liver enzymes).The results indicated that maternal age had no significant effect on the level of adiponectin, apelin, FSH and LH hormones, while the study showed a significantly effect on level of progesterone, estradiol, testosterone and insulin hormones between pregnant and non - pregnant women. Adiponectin levels decreased in pregnant and non - pregnant females with increased body mass index whereas apelin, estradiol, progesterone and insulin levels increased. When the comparison between pregnant and non - pregnant women was done, a decrease in adiponectin and apelin was noticed. Accompanied by an increase in the level of estradiol, testosterone, progesterone and insulin hormones in pregnant women compared to non - pregnant women.Women with PCOs showed low levels of both Adiponectin and apelin with high levels of Testosterone and Insulin.Results also revealed that age had no effect on total cholesterol, triglycerides, and HDL, in both pregnant and non - pregnant females, but when the BMI was taken in consideration, high levels of these parameters were found in - group of BMI (obese). In addition, the present study showed that age had no effect on liver enzymes (AST and ALT) while ALP showed that the behavior increased up as a different high body mass index in pregnant women, in comparison with the control group. However, ALP activity also increased in high BMI group.Hematological study showed that age had no effect on RBC, Platelets, and hematocrit. Similar results were found when BMI was taken in consideration. Total WBCs showed a non - significant increase in pregnant women as compared with the control group. Adiponectin levels were significantly higher in pregnant females carrying female fetuses compared to those with male fetuses, while the other hormone apelin did not differ between the two groups. The Result of the present study revealed that adiponectin was significantly decreased as apelin was significantly increased with the increased parity.

دراسة مسحية للبكتريا المرتبطة بالتهاب الزائدة الدودية وتحديد الحالة النسجية للمصابين في محافظة البصرة == Survey of bacteria associated with appendicitis and determination of Histological patient state in the province of Basrah

Author name: هدى عبد الرحيم مذكور
Supervisor name: محمد عبد الامام احمد | نوري حنون جاسم
General topic: Biology
Specific topic: Microbiology - Bacteria
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study has been done to determined pathogenic bacteria that associated with appendicitis .This study includes ninety samples of removal appendix taken form patients who cleared diagnosed as appendicitis infection by specialised doctors in general Basrah hospital and Al - Sadir teaching hospital for the period between September - 2013 and June - 2014.Distribution of appendicitis appear in all age groups, 10 - 20 year and 20 - 30 year were the most infected, as well as the infection appear in both gender, but rate of infection in male was relatively higher than female 68(59%), 47(41%) respectively. The ratio of the city's population has overcome the rural population 96(83.5%),19 (16.5%) respectively. The percentage of samples that gave positive culture was 80( 88.9%), while 10 (11.1%) of these samples negative culture. The study reveals 15 different bacterial isolation, the most common bacteria was Escherichia coli 80(44.9%) while other species was appears in less percentage Shigella dysenteria 14(7.9%), Salmonella enterica typhi 10( 6.5 %), Pseudomonas aeruginosa 8( 5.6 %) , Klebseilla Pneumonia 6(4.3%) , Klebseilla oxytoca 3(1.7%), Morganella morganai 7 ( 9.3 %) Neisseria spp. 6 ( 9.5%) , Enterobacter cloaca 4 ( 2.2 %) ,Serreatia spp. 3 )7.1 %(, Citrobacter ferundii 2 (7.7%) Proteus vulgaris 7) 6.5 %( Staphylococcus spp. 14(1.3%), Streptococcus spp. 72(5.1 %) and Bacillus subtilis 8)5.6 %(. Laboratory diagnosis for blood samples included estimated of total WBCs and found that (31%) of patients have natural WBCs values while the other patients have high values.Antibiotic sensitivity test to E.coli isolates towards 20 antibiotics because she is the most common bacteria showed that all isolates were resisted for most antibiotics which used in test especially for β - lactam group, and the isolates of E.coli were multi resistance for antibiotics.Identified minimum inhibitory concentrations MIC for some antibiotics The results showed that E.coli bacterial isolates's 30 isolation have shown resistance to anti AMO as MIC values ranged from 128 - 1024 Mcgm / ml while the MIC for Anti AMP values between 4 - 128 Mcgm / ml, As for antibiotics and Cefixim , Ceftriaxone, Cefotaxime, and it was her MIC values ranging between (1 - 128) Mcgm / ml Plasmid profile of E.coli isolates investigated to study the correlation between plasmid profile and antibiotic resisitant marker and results from agarose gel electrophoresis revealed that all E.coli isolates contain one plasmid band.This study includes the detection of some genes that encode to beta - lactamase enzymes in E.coli which were responsible about multi antibiotic resistanc and these genes loaded on plasmid DNA for ten isolaes and found that 5(50%) from isolates have blaTEM gene and 5 )40%( have blaCTX gene and 1(10%) have blaSHV gene. This study also considered note the general appearance of appendix samples , some of them are enlarged and surrounded by vesicles, some with fibrous walls and ulcerated with mixed colors, then examined the histological changes it, the study showed changes in histological structure of the excess was extensively congestion of blood vessels, veins in serosa and subserosal layers and increased the amount of diffuse lymphoid tissue in the layers of the appendix walls.

دراسة فعالية بعض مستخلصات الطحلب الاخضر Enteromorpha intestinalis (L.,1820 على الاكياس العذرية للمشوكة الحبيبية Echinococcus granulosus (Batsch,1786 وكمضادات لخط الخلايا السرطانية نوع HeLa == Study of the activity of some of the green alga Enteromorpha intestinalis (L.,1820) extracts on Hydatid cysts of Echinococcus granulosus (Batsch,1786) and as anticancer cells type HeLa

Author name: غزوان طالب نوري الجابر
Supervisor name: صبيح هليل المياح | احمد محسن عذبي
General topic: Biology
Specific topic: Biotechnologies
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The present study was conducted in order to evaluate the activity of green alga Enteromorpha intestinalis extracts (methanol and hexane extracts) on growth and development of hydatid cysts of Echinococcus granulosus in vitro and in vivo by using mice type Mus musculus Balb\c that in comparing with albendazole drug. Anti - cancer activity was also evaluated in vitro for both algal extracts by using HeLa cells. Chemical compositions of algal extracts were analyzed by using Gas chromatography - mass spectroscopy (Gc - mass)technique . The hydatid cysts were collected from livers and lungs of infected sheep which slaughtered in Basrah abattoir. While, The algae specimens were collected from Abu Sokhir marshland area /north of Basra. The results revealed that E.intestinalis chemical compounds are carbohydrate , alkaloids, phenols , saponins , triterpenes and sterols compounds. In vitro study showed that the 1 mg / mouse weight of albendazole drug was able to reduce the percentage of protoscolices activity to zero after the third day of treatment. While, the dose 6 98 mg / kg of methanol extract inhibited all protoscolices after five days of treatment, followed by the dose 804 mg / kg of hexan extract that inhibited all protoscolices after six days of treatment.In vivo study revealed that the methanol extract (698 mg / kg) treatment was the best in reducing the weights of organs significantly in comparison with the positive control group it was 2.192 , 0.192 , 0.434 and 0.282 g of liver, spleen, lung and kidney respectively. The methanol extract (698 mg / kg) treatment lowered the average number of hydatid cysts (2.6) more than other extracts treatments , then followed by hexane extract (804 mg / kg) treatment it was 2.8 cyst .This study has identified that the liver enzymes Aspartate transaminase (ALT) and Alanine transaminase (AST) values in the serum of positive control group were higher significantly than negative control group the value of the enzyme ALT was 79 IU / l and the value of the enzyme AST was 118.4 IU / l.All methanolic extracts groups showed a preference in enzyme's values compared with other treatments groups , the value of ALT enzyme of 698 , 688 and 678 mg / kg of methanol extract groups were 46.8 , 45.8 and 44.6 IU / l respectively, while all hexane extracts groups showed a preference in AST enzyme values compared to the other treatments groups, the value of AST enzyme of 804 , 794 and 784 mg / kg of hexane extract groups were 73.6 , 70.2 and 70.4 IU / l respectively. Found a significant increase in the number of white blood cells WBCs in a positive control group ( 4.6 × 013μ), compared with a negative control group and the other groups, While it has been found significant decrease in the Hb (5..8deciliter) and Packed cell volume (PCV) (31..%) values in positive control group as compared with control negative group. The study found that the number of white blood cells WBCs n the methanolic extract group (678 mg / kg) was 4.06 × 103 microliter was as same as in the negative control group, In contrast, hexane extract group (804 mg/ kg) has did not significant difference with negative control group in the Hb and PCV were 11.5 g / dl and 36.8% respectively .Histological study confirmed that there are the histological changes in positive control group included congestion , bleeding , degeneration , infiltration of inflammatory cells and hyperplasia. Whereas the histological changes in group treated with albendazole were bleeding, infiltration of inflammatory cells , atrophy of the glomerulus and fibrosis in some areas . In algal extracts groups the histological changes were infiltration of inflammatory cells, bleeding and congestion.The Gc mass results revealed that the ethanol extract contains loliolide , ethyl stearate , palmitic Acid , ethyl palmitate , phytol , ethyl oleate , ethylhexyladipate and squalene compounds. While, hexane extract has N, Ndimethyltetradecylamine , diisobutyl phthalate , palmitic Acid , ethyl palmitate and ethylhexyladipate compounds.Anti - cancer study showed the algal extracts have an inhibition activity against cancer cells, The half maximal inhibitory concentration (IC50) value of the methanol extract was 79.08 μ/ ml, While, the IC50 for hexane extract was 156.3 μ/ ml.

دراسة تشريحية تصنيفية لبعض من نباتات العائلة السوسبية Euphorbiaceae في العراق == Anatomical and Taxomanical study for some plant of Euphorbiaceae family in IRAQ

Author name: امل علي ياسين الحسن
General topic: Biology
Specific topic: Plant
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted to investigate several taxonomic aspects which comprised the micro - morphological studies of epidermal layer of leaves, pollen grains, fruits and seeds.In addition to that, the anatomical and molecular studies for 12 species of Euphorbiaceae family which belonged to five genera AndrachneL. , Chrozophora L., Euphorbia L, Mercurialis L. and RicinusL., were performed. One representative species for each genuswas selected; in exception 8 species were chosen for the genus Euphorbia..The current results confirmed that the anatomical characters showed valuable taxonomic traits which can evidently be used to distinguish between species. The most prominent trait was mesophylla tissue in leaves which were observed in three categories;1 - Bifacial leaves : - which found in C.tinctoria, Euphorbia sp ., E. milii, E.annua and R.comminus 2 - Uni - facial (heterogenus) leaves : - this category of the mesophyll was detected in two species only A.telephioides and E.prostrata3 - Uni - facial (homogenus) leaves : - this category were noticed in five species; E.denticulata ,E.helioscopia ,E,macrocarpa , E.microsphaeia and E.peplus The second important comparative anatomical character was the Mid rib, which are in four types with several shapes from which discrimination between the species can be made : - 1. Convex - concave mid rib : - that was showed in E.helioscopia, E.macrocarpa, E.milii and M.annua.2. Convex - convex : - This type was found in three species C.tinctoria , Euphorbia sp.and R. comminus3. Flat - Convex : - This type of mid rib was observed in A.telephioides E.microsphaera, E.peplus and E.prostrata4. Flat - Flat : - This type was observed in only one species E.denticulata In stem, the most prominent parts of taxonomic evidence were pith and vascular bundles.In according to the pith, species can be divided into hollow stem (without pith) and solid stem (containing medulla good configuration). Hollow stem was clear in the stems of A.telephioides , E.microsphaera , E.prostrata and M.annua. While, the solid stem wasseen in the remaining species. Vascular bundles were existed in two types; that's connected vascular bundles that's characterized the hollow stems, and separate vascular bundles. In most of the species with the exception of M. annua, stem with scatteredvascular bundles were in cortex As well as, petiole which have features that a clear taxonomy values .either by outline of petiole or its vascular arc.Micro - morphological studies of epidermal layer of leaves showed very important taxonomical value. This layer of leaves reveals variation in shape of ordinary epidermal cell and nature of anticline cell wall, stomata complexes and Epicuticlular wax crystals.Micro - morphological of pollen was taxonomic evidence as well. It can be considered to divide the species of Euphorbiaceae family into two groups based on the number of germination apertures 1 - Hexocorporate : - this type was confined to C.tinctoria2 - Tricolporate all species studied remainingThe sculpture of pollen wall provided beneficial information invested in the species identification.Both seeds and fruits showed a distinctive role in species identification. Micromorophological study of seeds for species which belong to six genera Andrachne , Chrozophora, Euphorbia, Mercurialis , Phyllanthus and Ricinus were achieved by using Scanning Electron Microscope(SEM). The study concentrated on micro - morphological aspect of Caruncle, seed surface ornamentation and the shape of epidermial cell wall. The result revealed that most studied species were with carunclated seeds ,but some specieswere with ecarunclated seeds as in A.telephioides L. ,C. tinctoria( L) Raf , E.prostrata Atin and P. maderaspatensis L Seed surface ornamentation characteristics showed a significant taxonomical value.Furthermore, current result showed that there are a highly variation in shape, size and color of the seeds walls. With respect to the fruits characteristics, which showed variations in number of mercarpi, shape, size, color and surface ornamentation that can be adopted in the separation of the species studied Molecular study provided significant support for phenotypic and anatomical classification and support genetic convergence between the species in Euphorbiacea family. The genus Euphorbia species showed a significant genetically convergence and participated in predecessors monophyletic. However, the genera in Euphorbiacea family under study probably originated and evolved from different ancestors’ polyphyletic.

دراسة تصنيفية وحياتية للديدان الخيطية عائلة Tetrameridae Travassos, 1914 المتطفلة على بعض انواع الطيور في هور السناف محافظة ذي قار == Taxonomic and Biological Studies of Nematodes of the Family Tetrameridae Travassos, 1914 in the Some Birds in Sinaph marsh Thi - Qar province

Author name: نهى جبار عبد الركابي
Supervisor name: باسم ھاشم عبد الله
General topic: Biology
Specific topic: Microbiology - Parasites
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: A total of 401 represented by 128 birds of Himantopus himantopus which belong to the family Recurvirostridae , 93 birds of Chettusia leucura which belong to the family charadriidae and 180 birds of Sturnus vulgaris which belong to the family sturnidae at the period from April 2014 to march 2015 in Al - Sanaf marsh , Suq - Al - Shuyukh city southern of the Thi - Qar province,for searching the nematodes which belong to the family Tetrameridae. Four species of nematodes, which belong to the family Tetrameridae, were isolated in the current study. They were T.nouveli, T.dubia, T.spinosa and Microtetrameres inermis. The H. himantopus was infected with the nematode T.nouveli and the prevalence of infection was 25.8% , bird.Furthermore, the Chettusia leucura was infected, the mean intensity was 3.3 worm in each infected birds. The nematode T.dubia was isolated from H. himantopus with the isolated from the Ch. Leucura mean intensity was 1.7.The nematode T.spinosa was isolated from H. himantopus the the mean intensity of infection was 2.3 and it was isolated from the Ch. leucura , the prevalence of infection 4.3% and the mean intensity was 1.8 worm in each infected worm.The record of these three nematode is considering the first record in Iraq.Microtetrameres inermis was isolated from the Sturnus vulgaris, the each infected birds.Himantopus himantopus from April 2014 to March of 2015.This bird was infected with three species from family Tetrameridae, The seasonal variation study of infection prevalence of Tetrameres spp.in, H. himantopus that there was II Summarycomplete seasonal cycle for nematodes T.nouveli, T.dubia , and T.spinosa .The nematode T.nouveli had hightest infection prevalence in summer the decrease in autumn, winter and spring while the infection prevalence of nematode T.dubia was hight in summer and autumn then decrease in winter and spring. The infection prevalence of nematode T.spinosa was generally low with little increase in autumn .The results of the current of the Ch. leucura indicated that this bird was infected with three species which nematodes T.nouveli, T.dubia and T.spinosa and they had seasonal appearance . There was not any significant effect of host sex on prevalence of infection, and it showed decreased in prevalence of infection when the bird's weight was increased.The study of lifecycle of nematodes : T.nouveli ,T.dubia and M. inermis revealed obtainment the larval stages after success the infection experimental of the grasshopper Schistocerca gregaria for the first time the three isolated nematodes from family Tetrameridae in this study which it was T.nouveli , T. dubia and M.inermis and was included the lifecycle of the three species . The differences were including the period of time to produce and developing the larval stages in addition to the differences in their shapes, it was revealed that were clear differences in the structure and their shape of the tip the posterior end of the third larval stage. The end of the third larval stage of the nematode T. nouveli had three caudal papilla and the posterior end of nematode T.dubia had five caudal papilla while the end of the third larval stage of M.inermis did not have any caudal papilla , so we can distinguish these nematode larval stage depended on the prevalence this character in their third larval stage.The experimental infection of the Columba livia domestica with nematode M.inermis was successful.While the experimental infection to the Columba livia domestica with nematode T.nouveli ,T.dubia .was failed The experimental infection of the Gallus domestica with nematodes of Tetrameridae T.nouveli, T.dubia and M.inermis was failed too.

دراسة تشخيصية وحياتية لعلقة المياه العذبة Dina punctata Johansson, 1927 (Erpobdellidae : Hirudinea في اهوار السويب من محافظة البصرة جنوب العراق == Identification and Biological study of the Freshwater Leech Dina punctata Johansson, 1927 (Erpobdellidae : Hirudinea) in AL - Swaib Marshes From Basrah province south of Iraq

Author name: ازهر محمد غالي الخزعلي
General topic: Biology
Specific topic: Zoology - Invertebrates
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The species Dina punctata is recorded for the first time in Iraq (from Al - Swaib marshes). The identification was conducted morphologicaly and molecularly characteristics. The morphological features summarized as : - - The species D. punctata has vermiform body compressed ventrally, ranging in length from 55 - 60 mm and in diameter from 0.5 - 2 mm. The individuals have a large ability for extening. The pointed anterior end carries small anterior sucker which resembles lips surrounding the mouth opining, while the rounded posterior end carries large rounded posterior sucker. - This species is characterized by heterogeneous features such as the color of the body which changes from blackish gray for Adults to dark brown for Juveniles, as well as the number and arrangement of eyes which is in general four pairs arranged as reverse U shape located on the dorsal anterior edge. The first two pairs of eyes are located on the middle line of the body while the third and the fourth pairs are each located on one side of the body behind the anterior pairs. - The body consists of 34 segments, each of them is divided to five secondary annuli. The fifth annuli of each segment is divided into two subsections, thus the main segment becomes consist of six annuli. - The male and female gonopores are open on the ventral surface of the 12th segment, separated from each other by two annuli, the male gonopore is usually larger and clearer and located forward in the middle of the groove which is separate the annuli b2 and a2 respectively. The female gonopore is smaller and located in the back of the male gonopore in the middle of the groove which is separate the b5 and b6 annuli respectively.Because of the instability in some morphological features, the species D.punctata also identified in the present study according to genetic features.During the genetic study, the region of internal transcription space (ITS2) in DNA was isolated from leech specimens by using special primers for region internal transcription space (ITS2), after isolation, the ITS2 genes are amplified.The results of polymerase chain reaction of DNA were obtained of single bands of 580 base pairs molecular weight for all degrees of hybridization. The sequence of each sample after detecting was entered in the database of National Center Biological Information (NCBI) and the result showed that the sequences of all samples are belonging to the species D. punctata. In the field study, monthly samples of leeches D. punctata was collected from the marshes of Al - Swaib on the eastern side of Shatt Al - Arab river inQurna northern Basrah Province during the period from January 2015 to December 2015. A total of 235 individuals and 623 Cocoon were collected during the study period. The highest number was 58 recorded for individuals during May 2015, while being 335 recorded for cocoons during February 2015. On the other hand there is no occurrence recorded during July - August for individuals, and June - November for cocoons.The study investigates the water temperature, salinity and abundance of individuals and cocoons. The statistical analysis showed negative correlation between temperature and abundance of both individuals and cocoons, while the correlation of salinity was slightly positive with abundance of individuals and cocoons.The study of population sizes structure showed that the size class 40 - 50 mm has the highest frequency during the study period compared with the other size classes. On the other hand, the highest percentage of frequency was 66.7%, which also recorded for the size class 40 - 50 mm.Laboratory experiments showed that the members of a species D.punctata able to tolerate saline concentrations between 2 - 6 psu the survival rate 100%, Also, these worms (D. punctata) feed by different ways, it can be considered Predatory Macrophagous and Scavengers, its food materials consist of invertebrate animals such as Oligochaetes, Lumbriculids, Chironomid larvae; fleshy body of some aquatic snails; and on dead fishes. In the laboratory, the leeches fed well on flesh and livers of chicken, sheeps and caws, The laboratory also studied, Three different types of food effect (chicken liver, zooplankton, and animal feed) on the growth of young leech for a period of six weeks.From the field and laboratory observations, the specie D. punctata reproduces sexually by cross fertilization. The male and female gonopores are easily observed in adults which ranging in length from 45 - 60 mm. as well as the sperm sacs on the mid ventral surface of the body during the reproduction season, which extend in our study during two periods : January - May and December 2015. The sexual reproduction processes were studded in detail and the embryonic development in cocoons was divided into five hypothetical stages according to morphological and anatomical changes and these stages are : - 1 - Depositing Cocoon Stage.2 - Primary Cleavage stage.3 - Development and extension.4 - Differentiation of regions stage.5 - Complement and release stage. The histological structure of the body wall, suckers, reproductive organs and digestive tract of adult leeches were investigated. The body wall consists of thick layers of circular, longitudinal and oblique muscles.The structures of the male reproductive system such as genital atrium, sperm sacs and testis were observed. There are two lateral blood sinuses around the mouth cavity in addition to many nerve cells. The posterior sucker is muscular, and the muscle cells are as a dense net in the peripheral part, while are as a loose cells in the middle what give it a sponge appearance, in addition to primitive glomerular cells, nerve cells and mucus cells

معاملة بذور الذرة الصفراء Zea mays L. بحامض السالسياليك واثرها في تحمل الشد المائي المستحث بمادة الكلايكول متعدد الاثلين == The treatment of Corn seeds (Zea mays L.) with salicylic acid and its effects on drought tolerance induced by polyethylene glycol (PEG)

Author name: وسن فوزي عبد الحسین
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: The study was conducted in Plant physiology laboratory, department of biology, college of education for pure sciences, University of Basrah from 1/6/2015 till 10/7/2016 in order to evaluate the effect ofpretreatment (soaking) seeds of corn (Zea mays L.) cv. Baghdad 3with salicylic acid (SA) on germination, early seedlings growth and to minimize the effect of water stress induced with poly ethylene glycol (PEG). The study included the following experiments : 1 - Effect of SA and PEG on germination using a hydroponic culture and Hoagland solution in which corn seeds were soaked in three concentrations (0.1,0.3,0.5) mMof SA in addition to control treatment with distilled water for six hours. Water stress were induced with PEG at concentrations of 0 (distilled water),1%,2%,3%,6%,9% and 12%. This experiment indicated that the optimum soaking concentration with SA was 0.5 mM and that there was no germination at 12% PEG.2 - Effect of interaction between corn seeds pretreatment with DW, 0.5mM of SA for six hoursand PEG at all previous concentrations except 12%, on early corn seedlings growth using hydroponic culture and Hoagland nutrient solution, for three weeks.3 - Effect of interaction betweencorn seeds pretreatment with DW, 0.5 mM of SA for six hoursand water stress induced with PEG at 3% and 6% on vegetative growth using pots filled with a mixture of sand and peat moss for three months.The parameters were evaluated after each of the previous experiments, also , Biochemical test . Plant height, leaves number, root length and number, dry and fresh weight for shoot and roots, while the followingparameters were evaluated in corn leaves after pots experiment : 1 - Biochemical tests included : proline and glycine betain concentrations, total carbohydrates, total chlorophyll, sodium and potassium ions concentrations.2 - Leaves anatomical study to compare the effect of PEG inducedwater stress and the role of its interaction with pretreatment with SA on thegrowth and development of internal structure of corn leaves.The following results were estimated : 1 - Germination percentage : increasing water stress in the nutrient media reduced the % germination significantly when compared with control treatment. Seeds pretreatment with SA enhanced the germination percent and the highest percent was at interaction between PEG at 1%, 2% and SA at 0.5 mM.2 - Growth parameters for seedling at hydroponic culture indicated that plant height, fresh and dry weight for shoot and roots have increased significantly when seeds were pretreated with 0.5 Mm SA All theprevious parameters were reduced significantly under PEG induced waterstress especially at 9%. Interaction between SAand PEG caused apositive significant increases in all studied parameters especially atconcentrations less than 6% and 9% PEG.3 - Growth parameters for corn plants grown at pots have showed a significant increase when pretreated with SA compared with that treated with DW. PEG caused a significant decrease in the growth parameters IIIespecially at 6%. Interaction between DW and 6% PEG recorded the lowest growth in all studied parameters. 4 - Biochemical tests : There was a significant increase in total carbohydrates and total chlorophyll content in leaves of corn plants pretreated with SA in compare with that pretreatdwith DW while the content of proline and glycine betaine were higher significantly in leaves pretreated with DW.In case of PEG induced water stress, the highest total carbohydrates content was at 6%, highest chlorophyll content at 3% while proline and glycine betaine highest content were at 6% PEG. 5 - Sodium and Potassium Concentration : The highest potassium concentration was at plant treated with SA while the highest sodium concentration was at plant treated with DW. Increasing PEG concentrations caused a significant increase in potassium concentration and a significant decrease in sodium concentration. Interaction results revealed that the highest potassium and the lowest sodium concentrations were at interaction between SA and PEG at 6%. 6 - Anatomical study results revealed that increasing water stress effected the leaves tissues structures and this were cleared at vascular bundles tissues (xylem and phloem tissues) and bundle sheath. Upper and lower epidermis, polliform cells were also affected by increasing water stress.The anatomy of leaves also showed that there was a positive enhancement in the structure of leaves tissues in the plants grown from pretreated seeds with SA.

استخدام البكتريا المختزلة للنترات المعزولة من مياة انتاج حقول النفط في البصرة في السيطرة على الانتاج الحيوي لغاز كبريتيد الهيدروجين المنتج بفعل البكتريا المختزلة للكبريت == Using of Nitrate Reducing Bacteria Isolated From Produced Water of Oil Fields in Basra in Control of Biogenic Hydrogen Sulfide Produced by Sulfate Reducing Bacteria

Author name: وجدان حسين عبد الصاحب التميمي
Supervisor name: كوثر هواز مهدي
General topic: Biology
Specific topic: Microbiology
Degree: Doctorate
Language: English
University location: Basrah
First pages:
Abstract: تضمنت الدراسة الحالية استخدام تقنية الاقصاء بالتنافس الحيوي للسيطرة على الانتاج الحيوي لغاز كبريتيد الهيدروجين H2S من قبل الجراثيم المختزلة للكبريت في مزارع الدفعة والمفاعل الحيوي, اذ ان اضافة النترات او النتريت يحفز نمو الجراثيم المختزلة للنترات التي تنافس الجراثيم المختزلة للكبريت على المصادر العضوية وتستقصيها. جمعت 40 عينة من مياه انتاج حقول نفط نهران عمر واللحيس في البصرة خلال 6 اشهر للفترة من اب 2013 الى كانون الثاني 2014, عزلت مزارع خليطه من الجراثيم المختزلة للكبريت والمختزلة للنترات من تلك العينات وحسبت اعداد الخلايا لكلا النوعين باستخدام طريقه العد الاكثر احتمالا وقد اظهرت النتائج ان اعداد الجراثيم كانت منخفضة اذ بلغت اعداد الجراثيم المختزلة للكبريت بين7 - 4 خلية/100مل في حين كانت اعداد الجراثيم المختزلة للنترات اقل من 4 - 2 خلية/ 100مل. كما تضمنت الدراسة الحالية ايضا تنقيه للجراثيم المختزلة للكبريت والمختزلة للنترات واجراء التشخيص الجيني بتقنيه التفاعل التسلسلي لانزيم البوليمريز PCR والذي من خلاله تم تضخيم الجين16S rRNA وحدد تتابعه للتشخيص الى مستوى السلالة وقد اظهرت تحاليل المجتمع الجرثومي ان عزلات الجراثيم المختزلة للنترات قد امتلكت تطابقا بنسبة 99% مع الانواع Pseudomonas stutzeri وP. putida وHerbaspirillum huttiense وBacillus licheniformis اما عزلات الجراثيم المختزلة للكبريت فقد اظهرت النتائج انها امتلكت تماثل 99% للانواعShewanella hafniensis وDesulfotomaculum acetooxidans وDesulfosporosinus orientis مع افتراض ان جميع العزلات هي سلالات جديدة عالميا. كما اظهرت نتائج المعاملة بالنترات او النتريت في مزارع الدفعة وجود تاثير مثبط قوي على الجراثيم المختزلة للكبريت اذ لم يزداد مستوى الكبريتيد في جميع التراكيز المستخدمة ( 200 و400 و600 و800 و1000) ملغم/لتر من النترات والنتريت فقد تراوح مستوى تركيز الكبريتيد بين 4.2 و21 ملغم/لتر عند المعاملة مع النترات و23 و5.2 ملغم/ لتر مع النتريت, وتطابقت هذه النتائج مع تركيز الخلايا في جميع القناني المعاملة, اذ تراوح بين 0.26 - 0.001 و0.003 - 0.17 مع النترات او النتريت على التوالي بينما كانت هناك زيادة معنوية في تركيز الكبريتيد الذي بلغ 121 ملغم/لتر وتركيز الخلايا بامتصاصية بلغت 0.84 في عينات السيطرة.كان للجراثيم المختزلة للنترات تاثير مثبط قوي على نمو الجراثيم المختزلة للكبريت وانتاج الكبريتيد عند اضافة المغذيات المشجعة لنموها بمختلف التراكيز, اذ ازدادت فعاليه الجراثيم المختزلة للنترات واستهلكت كل من النترات او النتريت المضاف خلال 3 - 2 يوم من الحضن وكان اعلى تاثير مثبط لانتاج غاز كبريتيد الهيدروجين عند التركيز 1000 ملغم/لتر من النترات اذ اظهرت النتائج وجود انخفاض معنوي في مستوى الكبريتيد الى34 ملغم/لتر في حين كانت اعداد البكتريا 34.8 خلية/مل بعد عشرة ايام من المعاملة. اظهرت نتائج معالجة الحموضة طويلة الامد باستخدام المفاعل الحيوي وجود انخفاض حاد في انتاج غاز كبريتيد الهيدروجين بنسبة 94% بعد اضافة النترات والنتريت خلال فترة 60 يوما من المعاملة ,الاضافة المستمرة للنترات والنتريت بحوالي ( 333ملغم/اليوم للنترات و133ملغم/اليوم للنتريت) ادت الى تثبيط الفعالية الحيوية للجراثيم المختزلة للكبريت وانتاج غاز كبريتيد الهيدروجين, اذ انخفض مستوى الكبريتيد من 155 الى 10 ملغم/لتر واعداد الخلايا اكبرمن 1100 الى 3.5 خلية/ مل بما يعادل 99.6% , بينما ازدادت فعالية واعداد الجراثيم المختزلة للنترات من 1100 الى اكبر من 1100 خلية/مل.اظهرت نتائج معدل تاكل عينات الحديد المطاوع في المفاعل الحيوي بعد 60 يوما ان اعلى معدل للتاكل كان 70.7 ملغم/ سم2/ سنه في المفاعل غير المعامل بالنترات والنتريت بينما انخفض معدل التاكل الى 15.8 ملغم/ سم2/ سنه بعد نفس الفترة من التعرض في المفاعل المعامل وبنسبة انخفاض بلغت 55% مقارنة مع المفاعل غير المعامل. | This study includes the used of Bio Competitive Exclusion technology (BCX) to control biogenic production of hydrogen sulfide (H2S) by Sulfate Reducing Bacteria (SRB) in batch cultures and bioreactors. The injection of nitrate (NO3) and nitrite (NO2) promotes the growth of nitrate reducing bacteria (NRB) which outcompete (SRB) on carbon sources. A total of 40 samples were collected from produced water of oil production facilities in Basra including Nahran Omer and Al - Lahis oil fields in a period time of 6 months from August 2013 to January 2014. Mix cultures of (SRB) and (NRB) were isolated from these samples, the two types of bacteria were enumerated by using most probable number method, the results show that the numbers of both bacteria were generally quite low, the viable count of (SRB) was between 4 - 7cell/100 ml whereas the (NRB) was between <2 - 4 cell/100 ml. This study also includes purification of isolates for both (SRB) and (NRB) and the genetic identification by polymerase chain reaction (PCR) technique through which the universal 16S rRNA gene of 1500 bp is amplified and sequenced for identification to the level of strains. Microbial community analysis showed that isolates of (NRB) were have identity of 99% Pseudomonas stutzeri, P. putida, Herbaspirillum huttiense and Bacillus licheniformis while isolates of (SRB) were have identity of 99% Shewanella hafniensis, Desulfosporosinus orientis and Desulfotomaculum acetooxidans with supposing that all isolates new world strains. The results of the treatment with nitrate or nitrite showed a strong inhibitory effect on (SRB) growth and (H2S) production, sulfide levels do not increase at all concentrations (200, 400, 600, 800 and 1000) mg/l of nitrate or nitrite range between 4.2 - 21 mg/l in treatment with nitrate and 5.2 - 23 mg/l in treatment with nitrite. This is consistent with the concentrations of bacteria in all treatment bottles which range between 0.001 - 0.26 and 0.003 - 0.17 in treatment with nitrate or nitrite respectively, while there is a significant increase in sulfide 121 mg/l and concentration of bacteria 0.84 in control as optical density. (NRB) has a certain inhibitory effect on the growth of (SRB) and (H2S) production when the (NRB) nutrient (nitrate or nitrite 200, 400, 600, 800 and 1000) mg/l were adding , the activity of (NRB) increases after treatment, and it consumed all nitrate or nitrite during 2 - 3 days of incubation, the strong inhibition of (H2S) production is at concentration 1000 mg/l of nitrate where the results showed that there was significant decrease in sulfide level 34 mg/l and number of bacteria, 34.8 cell/ml during 10 days of incubation.Souring control at long term treatment in bioreactors show sharp decrease in production of (H2S) to 94% after injection of nitrate and nitrite during the period of 60 days, the continuous dosing of nitrate and nitrite (333 mg/l/day nitrate and 133 mg/l/day nitrite) inhibited the metabolic activity of (SRB) and (H2S) production severely, the sulfide levels decrease from 155 to 10 mg/l and number of (SRB) from >1100 to 3.5 cell/ml the percent in number decreased was to 99.6%, at the same time activity and numbers of (NRB) increase from 1100 to >1100 cell/ml over the duration of experiment. The results of corrosion rate measurement on mild steel after 60 days showed that the highest corrosion rate was 70.7 mg/cm2/year in control reactor while the lowest corrosion rate was 15.8 mg/cm2/year in treated reactor at the same period time of exposure with percent of decreasing in weight loss to 55% comparing with control reactor.

دور مستارجات حشرتي الصرصر الامريكي Periplaneta mericana والبعوض Culex pipiens على فرط التحسس من النوع الاول وعلاقتها مع بعض الائل مستضدات معقد التوافق النسيجي من الصنف الثاني

Author name: هدى كاظم كريم الموسوي
Supervisor name: فوزية علي عبد الله | ضياء خليف كريم
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: Arabic
University location: Basrah
First pages:
Abstract: This study aimed to verify the distribution of Periplaneta americana and Culex pipiens allergy and its association with some HLA class II alleles in asthmatic and allergic patients. 96 healthy (control ) and 96 suspected allergic individuals from the same geographical region ,paired by sex and age were included in this study. Enzyme Linked Immunosorbent Assay test (ELISA) was used to estimate the total and specific IgE antibodies in the sera of studied individuals. The detection of the HLA - DQB1*0602, HLADQB1* 0604 and HLA - DRB1*12 was performed by Polymerase Chain Reaction (PCR).The results of total IgE based ELISA which was conducted on 96 suspected allergic and24 healthy (control ) individuals revealed that there was extremely significant statistical association (P<0.0001) for total IgE concentration with being allergic or non allergic status of individuals. The overall frequency of higher total IgE antibody concentration ( IgE>200IU/ml) was observed in 72.9% of allergic patients and lower serum total IgE levels (IgE<200IU/ml) were associated with 87.5% of healthy individuals. According to the specific IgE based ELISA results the effect of allergic patients age on the IgE seropositivity against cockroach allergens was not considered to be significant(p>0.05),but the effect of sex was statistically significant)P<0.05(.There was no significant effect( p>0.05)for patients age and sex on the distribution of IgE antibodies against mosquito allergens. Out of 96 allergic patients, 59 (61.5%)and 62(64.6%)showed positive IgE antibody response against cockroach and mosquito allergens respectively. Concerning the PCR results the overall frequency of HLA - DQB1*0602 and HLA - DQB1*0604 alleles was %35.6 and %15.3 of cockroach allergic patients respectively and the HLA - DRB1*12 allele was not observed in these patients..Out of 62 mosquito allergic patients, 8(%12.9)showed HLA - DQB1 * 0602 positive results while HLA - DQB1*0604 allele was observed in 4(%6.5)patients and the HLA - DRB1*12 allele was not observed in these patients.The difference in the genotypig results was extremely highly significant(χ2 : 0.030;17.486; degrees of freedom(DF) : 3;p - value=0.00056136) between cockroach and mosquito allergic patients .The association between the presence HLA - DQB1 *0602 , HLA - DQB1 * 0604 and HLA - DRB1*12 and total IgE based ELISA results in allergic patients and healthy individuals revealed that the percentage of HLA - DQB1 * 0602, HLA - DQB1 * 0604 and HLA - DRB1*12 alleles presence in patients having total IgE lower total IgE )IgE<200 IU/ml) was higher than that of high )IgE>200 IU/ml) total IgE patients where HLA - DQB1*0602 appeared in high percentage(69.2%) followed by percentage (15.4 %( of HLA - DQB1* 0604 presence. . The relationship of HLA - DQB1and HLADRB1 alleles with the results of total IgE based ELISA was statistically significant in the least(χ2 : 60.756 and the degree of freedom (DF) : 5;p= P<0.00001) and greatest(χ2 : 11.806 ; the degree of freedom (DF) : 5 ; p = 0.037544) than 200 IU / ml, values .Depending on the association between the presence of HLADQB1* 0602 ,HLA - DQB1*0604 and HLA - DRB1*12 and positive or negative specific results of cockroach or mosquito allergens specific IgE based ELISA results in allergic patients revealed that the percentage of HLA - DQB*0602,HLA - DQB1*0604 and HLA - DRB1*12 alleles presence in cockroach allergic patients having positive results were higher than those ratios that have been observed in patients with negative ELISA results where HLA - DQB1*0602 appeared in high percentage)35.6%( followed by percentage( 15.3% )of HLA - DQB1*0604 . The relationship of HLADQB1and HLA - DRB1 alleles with the results of specific IgE based ELISA was statistically significant (χ2 : 10.412; DF : 3; p - value =0.01536989).Incontrast the percentage of HLA - DQB1*0602,HLADQB1*0604 and HLA - DRB1*12 alleles presence in mosquito allergic patients was found to be higher in negative ELISA results patients also the relationship of these HLA - DQB1 alleles with mosquito allergen specific IgE based ELISA results was not considared statistically significant (χ2 : 3.115 ;DF : 3 and p= 0.37423205).However HLA - DQB1 * 0602 allele was found in the highest percentage (20.6%), followed by allele HLA - DQB1 * 0604 (8.8%).Concerning the presence of HLA - DQB1*0602 ,HLA - DQB1*0604 andHLA - DRB1*12 in cockroach or mosquito allergic patients and healthy individuals,the HLA - DRB1*12 and HLA - DQB1*0602 allels were not found in healthy individuals.The genotyping results difference between cockroach allergic patients and healthy individuals was considered statistically extremely significant (χ2 : 74.485 ;DF : 5 and p =0) as well as the difference in the results of the genotyping of mosquitoes allergic patients and healthy people was statistically with very high significant (χ2 : 23.342 ; DF : 5 and p = 0.00029035).

استخدام الاسماك الذهبية Carassius auratus كدلائل حيوية لتلوث هور الحمار في البصرة

Author name: هدى حسن خربيط الخيون
Supervisor name: اياد حنتوش داود الخفاجي
General topic: Biology
Degree: Master
Language: Arabic
University location: Basrah
First pages:

دراسة تاثيرالتعرض السابق لمادة الايثيلين وايضه في بعض المعاييرالفسيولوجية : دراسة تاثيرالتعرض السابق لمادة الايثيلين وايضه في بعض المعاييرالفسيولوجية والوراثية عند العاملين في معمل البتروكيمياويات في محافظة البصرة والوراثية عند العاملين في معمل البتروكيمي == The Study of the effects of previous Ethylene Exposure on Some Physiological and Genetic Parameters in Workers of Petrochemical Plant In Basrah province

Author name: هبة ثاقب يسر
Supervisor name: سامي جبر المالكي | فائزة عبد الوهاب احمد
General topic: Biology
Specific topic: Life Science
Degree: Doctorate
Language: Arabic
University location: Basrah
First pages:
Abstract: The current study was conducted to investigate the genetic and physiological effects of some industrial chemicals on workers of the Petrochemicals Industrial Factory, in Basra Province , as it has been collecting 100 blood samples during the month of January and February of 2011 from each of the exposure in (ethylene section) and the control group of (employees of the University of Basra). and then the samples were divided on the basis of each of the years of occupational exposure to more and less than 15 years , age group to the larger and smaller than 50 years, smoking habits.The results showed that there was a significant decrease in the haemoglobin concentration HB, PCV, RDW and MCV in the more than15 years exposure group in comparison with the control group, while, no significance was detected in the RBC, MCH, MCV ,RDW,MCH and MCHC in less than15 years exposure group . Whereas, a significant decrease in the PCV for the same group was observed.White blood cells parameters’ results showed also variations in their values. In general, WBC did not change significantly in both groups of workers, while, the proportion of the lymphocyte and the of monocyte declined significantly in the more than 15 year of occupational exposure group, in contrast, the proportion of granulated cells increased markedly in the same group. The results of group of less than 15 years exposure revealed that the proportion of monocyte dropped significantly. Conversely, there was a significant increase in the proportion of granulated cells while the proportion of the lymphocyte was not significant . Platelets’ number and average of platelets’ volume decreased significantly in both occupational groupsTurning to the biochemical tests, the results showed significant increase in both liver enzymes ALT ,ASTand bili as well as this increase was found in urea, uric acid and glucose values in both occupational exposure groups. Contrariwise, the ALP creatinine and cholesterol did not reveal significant changes in both groups that in comparison with control group. The total protein decreased significantly in the group of more than 15 year of occupational exposure.Testosterone level revealed a significant decline in both occupational groups, while the Follicle stimulating hormone showed a significant increase in the occupational group of less than 15 year and no difference was observed in another occupational group while LH hormone level was not significant.In case of the genetic effects of the ethylene compounds, DNA was extracted for control and occupational groups and amplification was performed for GSTM1, GSTT1 and Albumin by using PCR technique. The results showed that there was no significant effect of both occupational periods on the GSTM1 gene, while GSTT1 gene was significantly in the workers samples in group more than 15 year of exposure, which was 2.43 times more than the group of less than 15 year of exposure.As regards the impact of exposure to ethylene and loss of genes GSTM1 and GSTT1 showed the results of our study, significant increase in the loss of gene GSTM1 amounted to more than five - fold (5.1) among workers exposed compared to a set of control while the proportion of loss of gene GSTT1 by more than three - fold (3. 1) compared to the group control has been a loss ratio of the two genes together more than eight - fold (8.5) for workers exposed group compared to the control.The light smoke did not reveal a significant effect on the null of GSTM1 and GSTT1 genes that in comparison with the control group, while the study found a significant effect of smoking on gene GSTM1, causing the null of much more than doubled (2.2) times compared with the control group (non - smokers) and the significant effect of smoking on null GSTT1 gene loss as increased visits by the text (1.5) compared with non - smokers also appeared that excessive smoking has an effect on the GSTT1 gene causing loss by seven - fold (7.08) once compared to non - smokers.The effect of the age group (less and more than 50 years old) of the workers on the null of both GSTM1 and GSTT1 was also examined. GSTM1 gene was null six times in both age groups of workers more than the control samples. While the GSTT1 was null in age group less than 50 years old three times more than the control group. As well as this absence was two times in the age group more than 50 years old in comparison with control group.It could be concluded from the present study that the exposure of workers to ethylene and its metabolic impact and clearly on blood standards as well as caused a high level of enzymes AST, ALT and bilirubin and high blood sugar levels and lower total protein and high level of urea and uric acid level as well as the low level of the hormone testosterone as it turns out that occupational exposure to ethylene and metabolic outcomes and length of exposure and smoking for workers exposed role in influencing the genes of detoxification, causing null in each of the gene GSTT1 and GSTM1.
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