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التشوهات الخلقية في اجنة الفئران السويسرية البيضاء من امهات معاملة بجرع مختلفة من عقار Mus masculus الديكساميثازون == Developmental anomalies in swiss albino mice embryos Mus masculus from the mother treated with different doses of Dexamethasone
Author name:
صدى غالب طاهر الموسوي
Supervisor name:
علي نعيم سلمان
General topic:
Biology
Specific topic:
Zoology - Embryology
Degree:
Master
University:
University of Thi-Qar - College Of Education For Pure Sciences
Language:
English
University location:
Dhi Qar
First pages:
24T3169 - p.pdf
Abstract:
The current study was conducted for the period from September 2015 until August 2016 in college of Education for Pure Sciences - University of Thi - Qar, this study aimed to fallow the embryonic lung development and evaluate histological changes in embryos's lung development when pregnant mice treated with different doses of Dexamethasone (Dex) and evaluate Alpha - fetoprotein (AFP) levels were evaluated as an indicator for congenital malformations. Sixty pregnant mice were randomly divided into four groups and each group contain 15 pregnant mice. The members of each group were given specific dose of Dex and at different time periods, while the control group injected with a solution of Normal Saline 0.9%.All animals received doses used by tail intravenous injection until the end of the time periods specified .Blood samples were taken for the purposes of biochemical parameters account as well as the collection of samples for histological study .The treatment of animals under the same conditions were determined with a dose based on body weight, according to what was stated in the pharmaceutical constitutions where's the therapeutic dosage was 0.002 mg \2mg (0.1mg.kg from body weight ). We used another two concentrations from drug ,one less than the therapeutic dosage (0.05mg \kg from body weight) and another more than the therapeutic dosage (0.2mg \kg from body weight ).The results have showed that was a negative effects on mice embryo's body weights and the lengths increase with increasing of number and doses concentrations. The using of different doses of Dex also showed various changes in general external morphological features and congenital malformation in embryos of treated mothers included : Death of embryos ,placenta damage ,neural Tube Defect ,Trunk Torsion ,Headhemorrhage, Brain hypertrophy ,Liver Hypertrophy ,Letter C Shape Embryos, swelling ,Convoluted Tail ,Short nostril and limbs, curved head, Wrinkled skin ,Crooked pave ,Short toes ,Seal legs appearance, Oedema ,absent fingers and eyelids ,Tail congestion and Bulging eyes. Histologically microscopic examination showed histological changes with varied severity and appearing between different sub experimental group compared with the control group.Rapid elongation in lung bud, advancing in lung development, the lung asflower appearance, Loosely lung mesenchyme ,necrosis in lung tissue ,mucus hypersecretion, elongation in epithelial air ways,epithelial Hyperplasia ,mesenchyme looks as butterfly in appearance in some parts ,thinning of alveolar walls ,thinning of the interstitialmesenchymal lung tissue ,elongation of developing alveolar septa ,free alveolar septa. Alfa - fetoprotein (AFP) levels showed different concentrations value in correlation with congenital malformations .when the (AFP) value was high there were neural tube defects and advancing in the lung development while they were low in normal lung development and in brain hypertrophy.
