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تاثير تمرينات بدنية ــ نفسية في تطوير تحمل السرعة الخاصة والانجاز وخفض القلق المتعدد الابعاد لعدائي (400م) حرة الشباب == The effect of psycho - physical exercises in the development of bearing special speed and achievement and reducing themultidimensional anxiety of hostile (400 m) free youth

Author name: عباس محسن عليوي الزهيري
Supervisor name: عبد العباس عبد الرزاق عبود الجازع
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

الصلابة النفسية ونسب اسهامها في بعض اساليب مواجهة الضغوط النفسية لدى لاعبي كرة القدم المتقدمين في المنطقة الجنوبية == Psychological rigidity and the proportions of theircontribution in some waysFacing the psychological pressure of football playersIn the southern region

Author name: حيدر عبد زوير شريف
Supervisor name: محسن علي السعداوي
General topic: Sports
Specific topic: Football
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

السلوك الايثاري وعلاقته بالتوجه نحو ابعاد التفوق الرياضي وتقدير الذات وترتيب الفرق لدوري اندية النخبة بكرة اليد == Ethical behavior and its relationship to the direction of dimensions of mathematical excellence and self - esteem and order teams for league elite clubs in handballs

Author name: جبار شاكر لفته
Supervisor name: عبد العباس عبد الرزاق الجازع
General topic: Sports
Specific topic: Physical Education
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

الاضطرابات المزاجية وعلاقتها بالذكاء الانفعالي لدى لاعبي بعض الالعاب الفرقية لمنتخبات جامعات المنطقتين الوسطى والجنوبية == Mood disorders and their relation to the emotional intelligence of players of some games Albarqih for the teams of universities in the central and southern region

Author name: حوراء محمد علي حسن السعيدي
Supervisor name: محسن علي السعداوي
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

استقصاء دلالات الفروق في اساليب التفكير وفق انموذج ستيرنبرغ بين لاعبي منتخبات الجامعات العراقية الحكومية للمنطقتين الجنوبية والفرات الاوسط ونسب مساهمتها بدافعية الانجاز == Investigation of the significance of differences in Thinking styles according to the Sternberg model among the Iraqi universities tems players in volleyball of the central and southern regions and their contribution to the motivation of achievement.

Author name: سمر راضي بشارة
Supervisor name: ميثاق غازي محمد
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

تاثير تدريبات الرمال على تطوير بعض القدرات البدنية و المتغيرات الفسيولوجية و انواع التصويب بكرة السلة للشباب == Effect of training on the sand to develop some physical abilities physiological variables and the performance of and offensive skills basketball youth

Author name: زيد محمد جبار
Supervisor name: امين خزعل عبد
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

تاثير تمرينات وقائية في بعض المتغيرات البدنية والميكانيكية والبايوكيميائية للسباحين الشباب == The effect of preventive exercises in some physical and mechanical variables biochemical for young swimmers

Author name: نوار مثنى صبحي الدراجي
Supervisor name: امين خزعل عبد
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

تاثير تمرينات خاصة باستخدام (الحبال المطاطية والاثقال) في تطوير بعض القدرات البدنية والمهارية للاعبي كرة اليد باعمار (15 - 17) سنة == The impact of special exercises using (rubber ropes and weights) in the development of some physical and skill capabilities of the handball players aged (15 - 17 years

Author name: زهراء حسون خليف
Supervisor name: جبار علي جبار
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

تاثير منهاج بانموذج بايبي في تعلم بعض المهارات الاساسية بالكرة الطائرة للطلاب

Author name: اسراء عبد الرضا يونس
Supervisor name: ميثاق غازي محمد
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

القيمة التنبؤية للاداء المهاري بدلالة بعض القدرات البدنية والحركية والقياسات الانثروبومترية كمؤشر لانتقاء ناشئي كرة الطاولة

Author name: صبا ليث اسماعيل الشمري
Supervisor name: ربيع لفته داخل الحجامي
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

تاثير استراتيجيتي دوائر التعلم والمجاميع المرنة في تعليم بعض المهارات الاساسية والتحصيل المعرفي بكرة القدم للطلاب باعمار 12 - 13 سنة == The Strategic Impact of Learning Circles and Flexible Groups with regard to Teaching Some of the Basic Skills and Epistemic Acquiring in the Football Game to the Students at the age 12 - 13 Years Old

Author name: ظافر جاسم حسين
Supervisor name: امين خزعل عبد
General topic: Sports
Specific topic: Fitness
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

اثر تمرينات بالتدريب الدائري البدني المهاري باستعمال ادوات مساعدة في تطوير بعض المؤشرات الفسيولوجية والقدرات البدنية والتصويب بكرة السلة للشباب == The effect of circular training by means of aids in the development of some physical abilities, physiological variables and types of correction basketball youth

Author name: محمد جبار منادي
Supervisor name: صادق يوسف محمد
General topic: Sports
Specific topic: Physical Education
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

تاثير اسلوبي التدريس التبادلي والتضمين وفقا لذوي المجال المعرفي (التاملي - الاندفاعي) في تعليم بعض المهارات الاساسية بكرة القدم للطلاب == the effect of the two modes of mutual learning and the inclusion of people in the cognitive field (meditative - impulse) in learning some of the basic skills of football for students

Author name: احمد حسين عبد
Supervisor name: صالح جويد هليل
General topic: Sports
Specific topic: Physical Education
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

تاثير تمرينات بوسائل تدريبية مساعدة في تطوير بعض المتطلبات البدنية والمهارية لحراس مرمى كرة اليد المتقدمين == Effect of Exercise by Using Helpful Training Means in Development of Some of Physical & Skill Requirements for Handball Goalkeepers

Author name: بهاء اذياب فيصل
Supervisor name: جبار علي جبار
General topic: Sports
Specific topic: Physical Education
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

الجرائم الماسة بسير العمل في التشريع العراقي : دراسة مقارنة == Offenses against the functioning of the Iraqi legislation A comparative study

Author name: صفاء فريد كاظم
Supervisor name: ناصر كريمش خضر الجوراني
General topic: Law
Specific topic: Criminal Law
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

موقف الصحافة العراقية من الحرب الكورية 1950 - 1953 == Iraqi press position of the Korean war 1950 1953)

Author name: غازي عيدان راضي حميدي الحجيمي
Supervisor name: ازهار عبد الرحمن عبد الكريم اللفته
General topic: History
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

مشكلات الري والانهار في مناقشات مجلس النواب العراقي 1925 - 1939 == Problems of Irrigation and Rivers in the discussions of the Iraqi Council of Representatives 1925 - 1939

Author name: ابتسام مهدي مطرود الزيدي
Supervisor name: مهند عبد العزيز عطية الشبيب
General topic: History
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

الحركات الاسلامية الشيعية في العراق 1958 - 1980 : دراسة تاريخية

Author name: كرار عبد الحسين جودة الخفاجي
Supervisor name: صالح جعيول جويعد السراي
General topic: History
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

الاحوال العامة للمتصوفة من خلال كتاب التشوف الى رجال التصوف لابن الزيات التادلي (ت627هـ/1230م) == The General Conditions of the Sufis through the Book of Tchuf to the Mystical Men of Ibn al - Zayyat al - Tadli,627 A.H / 1230 AD

Author name: انتظار خلف خضر العقيلي
Supervisor name: جنان جودة جابر العنزي
General topic: History
Specific topic: Islamic History
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

بن يوسف بن خدة واثره في السياسة الجزائرية 1943 - 1962 == Ben Youssef bin Khadh and his influence on Algerian politics 1943 - 1962

Author name: حسام صبار زبالة الذرب
Supervisor name: عباس حسين مجيسر الجابري
General topic: History
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:

التحري عن بعض المعايير المناعية وتعدد الطرز الوراثية لجين IL - 13 في مرضى الربو في محافظة ذي قار == Investigation of some Immunological parameters and polymorphism of IL - 13 gene in patients of asthma in Thi - Qar province

Author name: هند حسين صالح الطائي
Supervisor name: علي نعيم سلمان
General topic: Biology
Specific topic: Zoology - Immunology
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: Asthma is a chronic inflammatory disease of the airways in which many cells and cellular elements play a role. Causes recurrent episodes of wheezing, breathlessness, chest tightness, and coughing. These episodes cause airflow obstruction, often reversible either spontaneously or with treatment. The current study was conducted at the Labs of college of Education for pure science and Al - Hussein Teaching Hospital in Thi - Qar province, during the period from October 2014 to May 2015. The study aimed to investigate role of polymorphism of IL - 13 gene in patients with asthma and to evaluate their immune status by measuring the levels of some immunological parameter immunoglobulins ( IgE) by enzyme - linked immunosorbent assay (ELISA) and (IgG, IgM ) by single redial immune diffusion (SRID) and measure the levels of interleukins (IL - 6, IL - 17) in the serum using enzyme - linked immunosorbent assay (ELISA), the study included test phagocytic cells on phagocytosis (coefficient of phagocytosis) and the total and differential count of white blood cells .A total of 100 patients with bronchial asthma (68 females) and (32 males) whose age lies between 17 - 62 years have enrolled in this study. DNA was extracted and RFLP - PCR was performed by using primers specific for the gene IL - 13, the results showed the presence of mutations in a48 sample out of 100 patients with asthma at site 1112C/T after using restriction enzyme BstUI. the statistical analysis showed correlation between the occurrence of the disease and the emergence of mutation (C / T) in the promoter region of IL - 13 gene when compared with the healthy control in population of Thi - Qar province .When it was compared the patient group with healthy control group there was a high significant increase (P ≤ 0.001) in serum IgE, IgG of patients with asthma compared with healthy control group. And no significant difference in serum IgM of patients with asthma compared with healthy control group. Decrease coefficientphagocytosis was significantly (P ≤ 0.001) in all patients with asthma compared with healthy control group. Levels of interleukin (IL - 6, IL - 17) measured in serum of 60 a sample of patients with asthma, and in the serum of 20 samples from healthy control group. The results showed a significant increase (P ≤ 0.001) in the levels of these interleukins in the serum of patients with asthma compared to the control group.Also there was increased in the rate of counting the total of WBCs and differential cell lymphocytes, eosinophil, neutrophil, basophil, and monocyte (P ≤ 0.001) in patients with asthma compared with the healthy people in the control group.

دراسة جزيئية ونسيجية مناعية للنساء المصابات بمرض سرطان الثدي في محافظة ذي قار,العراق == Molecular and Immunohistochemical Study in Women with Breast Cancer in Thi - Qar Province

Author name: هبة لفتة عبد الغالبي
Supervisor name: منال بادي صالح | مها شاكر حسن
General topic: Biology
Specific topic: Life Science
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: Breast cancer is a complex and multifactorial disease resulting in abnormal cell growth that leads to malignant tumor formation.In this study we focus on mutations of breast cancer susceptibility genes 1 and 2 (BRCA1 and BRCA2), immunohistochemical evaluation of estrogen, progesterone, HER - 2 receptors, ABO blood groups , white blood cells and clinicopathological parameter ( age, breast feeding, family history and tumor site) .Eighty five blood samples were taken from patients who attended Al - Hussain teaching hospital and oncology unit in Al - Habboby hospital during the period (from August /2014 to April /2015) , fifty blood samples were collected from healthy women as a control group who attended Al - Hussain teaching hospital and divided into groups according to age, family history, age at first brith, breast feeding. For histopathological and immunohistochemical analysis fifty tissue samples were collected from patients with breast cancer who were undergoing surgical resection (mastectomy). Blood samples were obtained by venepuncture , using a 5 ml disposable syringe , and they were divided into three aliquots, which were 1 ml, 2 ml and 2ml, for collect serum, genetic studies and hematological parameters (leucocycte count and ABO blood groups) study. The tissue samples were collected from the 50 patients with breast cancer for histopathological diagnosis and for Immunohistochemical analysis (estrogen receptor, progesterone receptor and here2/nue) The total patients were divided according to their age in to five groups (30 - 39), (40 - 49), (50 - 59), (60 - 69) and (≥70 years). results of present study revealed that a highest incidence of breast cancer (41%) occur in the age oup between 40 - 49 years, there is a significant difference among patients groups (P ≤ 0.01).The results of our study showed that (77) of patients lactating and from those according to period of lactation we found that the highest percentage of patients (37.6%) were lactating for period between (18 - 22months) and the lowest percentage (10.38%) lactating for period between (1 - 6 month). Family history of breast cancer indicates a strong association with risk of developing breast cancer. The result of the present study revealed that the family history positive in 31 cases (36.47%), and 54 cases (63.52%) had negative family history of breast cancer.The results of present study showed that (41.5%) had first birth at age (20 - 29years), (23.37%) had their first birth in age group (15 - 19 years) and (30 - 39 years), and (9.09%) had first birth at age (≥40 years).Regarding the tumor site, we found that most of patients (50.5%) the tumor mass was located in the right side and (38.8%) cases the tumor mass were located in the left side.The DNA was extracted from blood samples by using Accupower® genomic DNA extraction kit. Each concentration and purity of all DNA samples have been measured by Nanodrop. Samples with a purity ranged from 1.2 to 1.8 have been enrolled in this study for the molecular detection of BRCA1/2 gene mutation in patients and control groups. Detection of BRCA1/2 gene mutation by multiplex PCR revealed that (185 del AG mutation in BRCA1 gene) was detected in 6 patients (7.05%) out of 85 cases with breast cancer, and (5382 ins C) mutation in the same gene was detected in 2 patients (2.35%). Regarding (6174 del T) mutation in BRCA2 gene was detected in 3 patients (3.52%). While this mutation was not detected in the control groups.Histopathologically, carcinoma was divided into 44 cases (88%) ductal carcinoma {from which 42 cases (84%) were invasive ductal carcinoma, and 2 cases (4%) were comedocarcinoma}, and 6 cases (12%) cases invasive lobular carcinoma. Results revealed that 2 cases (4%) were stage I, 13 cases (26%) were stage II, 24 cases (48%) were stage III and 11 cases (22%) were stage IV, also our results showed that the 2 cases (4%) were grade I, 23 cases (46%) were grade II and 25 cases (50%) were grade III. The results of immunohistochemical analysis for breast cancer samples showed that Estrogen receptors were present in 36 cases (72%), and 14 cases (28%) were negative, while Progesterone receptors detected in 27 cases (54%), and 23 cases (46%) were negative, on the other hand the expression of HER - 2/neu showed positive results in 28 cases (56%), and 22 cases (44%) were negative.Regarding the ABO blood groups, we found that most of the cases carry blood group A (40 cases, 47.05 %), and the lowest frequency was for blood group AB (7 cases, 8.23%).The results of the present study showed that leukocytes count, lymphocytes count, monocytes count, neutrophils count and basophiles count were lower in patients than the control group, with a significant difference (p value ≤ o.o1). While the eosinophils count was significantly higher in patients than the controls (p value ≤ o.o1).

تقييم كفاءة بعض المرشحات كبدائل في معالجة مياه الصرف الصحي واعادة استخدامها في ري نبات الذره الصفرا ء Zea mays L == Evaluation Efficiency For Some Alternatives Filters in Sewage Treatment and Reusing in Irrigation Plant Zea mays L

Author name: منى محمد نجیب
Supervisor name: حسين يوسف خلف الركابي
General topic: Biology
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: recently Iraq where suffer from several crisis including diminished quantity and deteriorated quality of water resources, specially in southern provinces. This study was designed to show the extent of using non - traditional water resources such as sewage after its treatment with local alternative chemicals and using the resulting water in agriculture sector. Samples were collected from the terminal tank of waste water station in Al Nasiriyah ( Alhindia station) according to the criteria of United States Environment Protection Agency (USEPA, 1977) regarding the sewage properties including pH, EC, total dissolved solid(TDS), total suspended solids( TSS), CO2, Mg+2, Ca+2, Na+, CaCO3, Cl - , PO4 - , NO3 - , chemical oxygen demand ( COD), biological oxygen demand (BOD5). Moreover, we easured three trace elements ( Pb, Ni, Cd) as well as sodium adsorption ratio( SAR) and biological properties and bacterial contamination with fecal coliform bacteria. Physicochemical analysis of both sewage and tab water was performed. It was found that pH values were 7.37 and 8 for sewage and tab water , respectively. While the electrical conductivity for tab water was 8.01 ds / m compared with that of sewage water which was only 1.76 ds / m . TDS, TSS and CO2 for both sewage and tab water were (3780, 723) and ( 800 , 320) and (5.41, 1.23) mg/ L respectively. Results also showed that sewage water contain high concentrations of cations and anions as compared to tab water. Magnesium concentration was 469.42mg/L in sewage water and 100.1mg/L in tab water while calcium was 581.2mg/L in tab water and 60.8 in sewage water. Sodium concentration was 662.86 mg/L in sewage water and 186.3mg/L in tab water. On the other hand, water hardness and alkalinity were (3300 , 280) mg/L for sewage water and ( 2.40, 66.49) mg/L for tab water respectively .Results of this study also showed an increase in the concentrations of some ions in crude sewage water with respect to tab water. Concentrations ofchloride,phosphate,sulphate and nitrate in sewage water sample were 2496.31 Mg/ L,1.6 Mg / L , 1976 Mg / L ,3.67 Mg / L and for tab water were 226.8,0.7,108.48,0.6 Mg/L, respectively. Fecal coliform number was 2600 cell/100 ml in sewage sample and only 6 cell/ 100 ml in tab water. BOD5 andCOD values were 18, 167 Mg/ L for sewage water and 2.0, 6.0 Mg/L for tab water respectively Heavy elements also found to be elevated in sewage with respect to tab water , where the concentrations of Pb,Ni& Cd in sewage sample were 2.8, 0.8 & 0.004 Mg / L , while they were 0.09, 0.001 &undetected in tab water, respectively. On the other hand, sodium adsorption ratio SAR in sewage samples was 7 and in tab water was only 0.48.Laboratory experiments dealt with the following : 1 - It was used sand filters as a primary processing for waste water to remove pollutants prior to pass it through used filter upon incubating it for 14 days . 2 - in this study , it was selected four types of filters to remove contaminants and improve the quality of water is (water plant Ceratophyllum demersum, wood sawyer , , coal and Cladophora glomerta ). As it was used as filters either aprimary step in processing waste water through passing these waters as aby - pass step these waters on sand filter was used in this studay .3 - chemical , physical and biological characteristics of waste waters were studied upon passing it on the under - studying filters and also estimated the efficiency of removal Removal efficiency (%) of the components. 4 - Used of Some international Standerds and Iraqi specifications to evaluate the quality of water befor and after to pass it on filters for irrigation purposes. Some of criteria Such as (Kloke, 1980; WHO, 1995; US EPA, 1996; European Society, 1996 and Awasthi, 2000. ) were used to in the evaluation of soil contamination with heavy metals.This study reached to the following results : .The study concluded accrediting wooden sawyer + sand and water plant in recycling and reforming waste water and its capacity to remove pollutants, evaluation of quality of waste water and the treated water after passing through the filters under studay results indicated that all types of water inclusive the class (C4S1 ) which means high salinity water with afew of sodium element according to the classification of Laboratory US salinity. and water to cause toxicity problem in the study, and showed It results that candidates contributed to the removal of pathogenic bacteria from the treated water, and irrigation with waters passed through ceratophyllum dermersum L and sand filter leading treatment gave the highest dry to plant maize and two shoot and root weight, Result of statistical analysis showed that soils characteristics had a significant effect on of dry weight, concentration and quantity absorbed heavy elements in sections shoot and root to plant maize, was not for irrigation with treated wastewater impact on soil pollution with heavy metals and the results showed that the soils environmentally safe after relying on the Organization standards (WHO) and the American Environmental Protection Agency (US EPA).

توصيف جرثومة Enterobacter sakazakii المعزولة من مصادر مختلفة في محافظة ذي قار == Characterization of Enterobacter sakazakii Isolated from various sources at Thi - Qar Province

Author name: مروى حسن نصير الصافي
Supervisor name: يحيى عبد الرضا عباس | راجي طعمة ناصر
General topic: Biology
Specific topic: Microbiology
Degree: Master
Language: English
University location: Dhi Qar
First pages:
Abstract: هدفت هذه الدراسة الى الكشف عن بكتريا Enterobacter sakazakii في بيئات مختلفة شملت عينات غذائية وبيئية وسريرية . اذ جمعت 310 عينة خلال الفترة من ايلول 2012 ولغاية تموز 2013.اظهرت نتائج الزرع الاولي على وسطVRBAواكار الماكونكي بعد التشخيص بالاعتماد على الصفات الزرعية والكيموحيوية وتوكيد التشخيص باستعمال العدة API 20E. ان 22 (7.09%) من اصل 310 عينة اعطت نموا لبكتريا Enterobacter كالاتي 9/80(11.2%) من بيئة المستشفيات ,12/180(6.6%) حليب اطفال,1/50(2%)من حالات الاسهال . توزعت العزلات بعد التشخيص المختبري على الانواع التالية (15/22) 68.1% Enterobacter cloacae,(4/22)18.1% Enterobacter sakazakii و3/22))13.6% للنوع Enterobacter aminogenus. اختبرت مقاومة عزلات Enterobacter sakazakii الاربع ضد 20 مضادا من اصناف مختلفة وقد اظهرت النتائج ا ن جميع العزلات كانت مقاومة على الاقل لست من المضادات قيد الاختبار.اختبرت قدرة بكتريا Enterobacter sakazakii على انتاج انزيمات البيتا لاكتيميز واسعة الطيف واظهرت العزلات عدم قدرتها على الانتاج واستخدمت طريقة القرص المصاحب لمعرفة قابلية العزلات على انتاج انزيمات الميتالو بيتا لاكتاميز.درست قدرة البكتريا على تكوين الغشاء الحيوي واظهرت النتائج ان 2/4(50%) من العزلات لها قدرة على انتاج الغشاء الحيوي.كما د رست قدرة البكتريا على مقاومة التاثير القاتل للمصل وظهرت العزلات كلها موجبة بينما درست قدرة البكتريا على تلازن كريات الدم الحمراء لوحظت تحت المجهر واظهرت النتائج ان 3/4(75%)من العزلات كانت موجبة.اختبرت قدرة البكتريا على انتاج السموم المعوية باستخدام الفئران الرضيعة وظهرت كل العزلات منتجة له وقد ظهرت على الفئران الرضيعة بالسموم المعوية اعراض سريريه حادة مثل انخفاض النشاط والحركة البطيئة وعلامات ضعف مع تغيرات في المعدة وخسارة التركيب النسيجي للغشاء المخاطي والغدد وظهور بؤر للخلايا المتحللة وزيادة في سمك الجدار مع تقرحات في النسيج اما التغيرات المرضية في الامعاء فهي تغيرات غير مخصصة تمثلت بتقطعات في النسيج الطلائي وتقرحه وفقدان لشكل النسيج والغدد. | This study was aimed for detection occurrence of Enterobacter sakazakii in stool specimens, infant formula and hospitals environments. A total of 310 samples (180 infant formula, 50 stool samples and 80 hospital environment swabs ), during the period from September 2012 to July 2013 were collected. Growth on Violet redbile agar and MacConkey agar was identified by cultural and biochemical tests and confermated by API 20 E system which revealed that : Only 22/310(7.09%)gave positive growth for Enterobacter spp. as following : 9/80 (11.2%) environmental swabs , 12/180(6.6%) infant formula and 1/50 (2%) stool samples. Diagnosis of the isolates appeared that E. cloacae was (15 ⁄ 22) while E . sakazakii (4 ⁄ 22) and E. aminogenus (3/22). Four E. sakazakii isolates were screened for their antibiotic resistance against 20 antibiotics of different classes using Kirby - Bauer disk diffusion method. The results showed that all the tested isolates were resistant to at least 6 antibiotics of which they were tested . In the present study four E. sakazakii isolates where screened for potential ESBLs production, they showed 3 (75%) isolates were potential ESBLs production ,but conformation of ESBLs production was detected by double disk synergism test and appeared that all the isolates were unproductive . Combined disk diffusion method was used for detection of metallo - β - lactamase - producing isolates, they were emplace each of imipenem and ceftazidame antibiotics with and without EDTA on Mueller - Hinton agar, because EDTA working to inhibit of metallo - β - lactamase. The results showed that the presence of EDTA does not affect the diameter of inhibition zone for the four isolates. Biofilm production was detected by spectrophotometric method .The four isolates were tested and appeared that 2(50%) were produced biofilm. The resistance of bacterial isolates to serum bactericidal activity was tested by the turbidmetric assay . All isolates were appeared able to resist to serum bactericidal activity, while Agglutination of erythrocytes was tested under the microscope and showed 3/4(75%) were positive. In the present study E. sakazakii were evaluated enterotoxin production. All the isolates showed positive for enterotoxin production. Results of treating suckling mice with enterotoxin showed acute clinical signs appeared depression, slow movement and signs of weakness. Significant macroscopic finding showed in mice treated with enterotoxin the stomach was distended and enlarged with thick corrugated mucosa but no significant changes in intestine. Pathological changes in the stomach appeared as loss of normal architecture of mucosa and glands, multiple foci of degenerative cells, reactive epithelial surface with thickening wall, pseudo - metaplasic changes and superficial epithelial injury. The intestinal changes showed nonspecific pattern of damage to surface of epithelium .Complete loss of architecture of glands and mucosal epithelial , superficial epithelial discontinuity , ulcer and non - specific reactive epithelial surface.

عزل وتشخيص الفطريات الجلدية من المرضى المصابين بداء السعفة في محافظة ذي قار ودراسة تاثير بعض العوامل في نمو تلك الفطريات == Isolation and identification of dermatophytes from patients with tinea in Thi - Qar Province and studying some factors effecting on their growth

Author name: سوزان خالد كاظم العسكري
Supervisor name: ياس خضير عباس | محمد حسين مشهد
General topic: Biology
Specific topic: Microbiology - Fungi
Degree: Master
Language: Arabic
University location: Dhi Qar
First pages:
Abstract: تضمنت الدراسة الحالية عزل وتشخيص بعض انواع الفطريات الجلدية المسببة لداء السعفة ((Tinea من المرضى المراجعين للاستشارية الجلدية في مستشفى الامام الحسين التعليمي في محافظة ذي قار , اذ جمعت 180عينة مرضية شملت 128عينة قشور جلدية و24عينة شعر و28 عينة اظافر من مرضى يعانون من داء السعفة (( Tinea للفترة من 13/8/2014 الى 1/1/ 2015 . بلغ عدد العينات المرضية الموجبة للزرع 110 عينة وبنسبة 61.1 % , اذ شكل عدد اصابات الاناث 74 وبنسبة 67.27 % , اما اصابات الذكور فقد بلغت 36 وبنسبة 32.73 % . بينت الدراسة ان الاصابة بسعفة الجسم (Tinea corporis) احتلت المرتبة الاولى من بين الاصابات وبنسبة 57.27 % ثم سعفة الراس ((Tinea capitis 17.27 % , ثم سعفة القدم 12.73 (Tinea pedis)% وشكلت سعفة الاظافر (Tinea unguium) بنسبة 9.09% , ثم سعفة اللحية (Tinea barbae)بنسبة 3.64 % . شخصت الفطريات الجلدية المسببة لداء السعفة باستخدام عدد من الاختبارات التشخيصية وقد وجد ان الفطر Microsporum canis اكثرها ظهورا وبنسبة 36.36 % يليه الفطــر Trichophyton tonsurans وT. verrucosum وM. gypseum وM. fulvum وبنسبـــة 27.27 % , 17.27 % , 12.73 % و6.36 % على التوالي . بلغت اعلى نسب للاصابة بسعفة الراس والجسم والقدم والاظافر في الاناث , في حين كانت اعلى نسبة للاصابة بسعفة اللحية في الذكور . وكان لعامل العمر تاثير في نسبة الاصابة , اذ احتلت الفئة العمرية (10≥) النسبة الاعلى في سعفة الراس , بينما كانت الفئة العمرية ( 31 - 40) النسبة الاعلى لاصابة سعفة الجسم والقدم واللحية اما سعفة الاظافر فكانت الفئة العمرية ( 21 - 30) النسبة الاعلى . بلغت نسبة الفطريات المفضلة للحيوان Zoophilic)) 53.6 % تلتها الفطريات الجلدية المفضلة للانسان (Anthropophilic) بنسبة 27.2 % , ثم الفطريات الجلدية المفضلة للتربة (Geophilic) وبنسبة 19.2 % . كما تناولت الدراسة تاثير بعض العوامل البيئية مثل نوع الوسط الغذائي ودرجة الحرارة والاس الهيدروجيني ومدة الحضن في نمو ثلاثة انواع من الفطريات الجلدية التابعة لجنس Microsporum وهي Microsporum canis وM. gypseum وM. fulvum . وقد اظهرت نتائج الدراسة ان افضل وسط غذائي لنمو الانواع الفطرية المعزولة هو Agar Sabouraud's Dextrose مقارنة مع وسطي Potato Dextrose Agar وCorn Meal Dextrose Agar , وكانت درجة الحرارة المثلى لنمو الفطريات الجلدية هي 25 م̊ . في حين كان الاس الهيدروجيني (pH = 6) للوسط الزرعي SDA هو الافضل في تنمية الفطريات الجلدية . كما اوضحت الدراسة زيادة معدلات اقطار المستعمرات الفطرية الجلدية النامية على الوسط الزرعي SDA عند pH = 6ودرجة حرارة 25 م̊ بزيادة مدة الحضن . كما تم استخــــدام تقنيــــة تفاعـــــل سلسلــــــة البلمرة ( PCR ) باستخــــدام البادئ ITS1 - 2FW - ITS1 - 2FV لتشخيص بعض انواع الجنس Microsporum . كما شملت الدراسة الكشف عن المواد الفعالة في المستخلص الكحولي لاوراق نبات اكليل الجبل ( Rosemarinus officinalis) اذ وجد ان المستخلص يحتوي على الفينولات والقلويدات والصابونينات والعفصيات والتانينات والراتنجات والكلايكوسيدات ولا يحتوي على الكومارينات. وقد تبين ان لهذا المستخلص قدرة تثبيطية ضد الفطريات الجلدية وبلغ اعلى تثبيط عند التركيز 200 ملغم / مل . وقد تباين التركيز المثبط الادنى (MIC) حسب النوع الفطري , فكان اقل تركيز مثبط ادنى للمستخلص الكحولي لاوراق نبات اكليل الجبل 25 ملغم / مل تجاه معظم الانواع الفطرية الجلدية , كما وجد ان المضاد الفطري Griseofulvin اكثر فعالية من المضاد الفطري Ketoconazole في تثبيط الفطريات الجلدية . | A study of isolation and identification of dermatophytes in Thi - Qar Province was conducted . A total of 180 clinical samples ( 128 skin scraping samples , 24 hair samples and 28 nails samples ) were collected from patients suffering Tinea and were consulting in Dermatology unit in AL - Hussein Teaching Hospital during 13/ 8/ 2014 to 1 / 1 / 2015 . 110 samples were positive for culture with a percentage of 61.1 % . Females infections were 74 cases ( 67.27 %) ,while male infections were 36 cases (32.73 %) . The study showed that Tinea corporis was the most Tinea infections with a percentage of 57.27 % . Tinea capitis , Tinea pedis , Tinea unguium and Tinea barbae had a percentage of 17.27 % , 12.73 % , 9.09 % and 3.64 % respectively . The isolated fungal species causing Tinea were Microsporum canis , Trichophyton tonsurans , T. verrucosum ,M. gypseum and M. fulvum with a percentage occurrence of 36.36 % , 27.27 % , 17.27 % , 12.73 % and 6.36 % respectively . Tinea capitis , Tinea corporis , Tinea pedis and Tinea unguium were high in females , while in males , Tinea barbae was the highest . Infection with Tinea was affected with patients age , so patients under 10 years old had tinea capitis more than others ; Tinea corporis , Tinea pedis , and Tinea barbae had the highest infection percentage in ages between 31 - 40 years . Tinea unguium had the highest infection percentage in patients with ages between 21 - 30 years . Zoophilic dermatophytes had the percentage of 53.6 % , Anthropophilic dermatophytes and Geophilic dermatophytes presented as 27.2 % and 19.2 % respectively . The effects of some environmental factors such as culture media , temperature , pH and incubation period on M. canis , M. gypseum . M. fulvum was studied .The best culture media for these dermatophytes was Sabouraud's Dextrose Agar comparing with Potato Dextrose Agar and Corn Meal Dextrose Agar. The optimum growth temperature was 25 oC , while pH = 6 was the best for culturing dermatophytes on SDA and 25 oC . Polymerase Chain Reaction (PCR ) with primer ITS1 - 2 FW - ITS1 - 2 RV was used to confirm the identification of Microsporum species . The alcoholic extract of Rosemarinus officinalis contained phenols , alkaloids , resins , tannins and glycosides , saponins . The extract had the highest inhibition activity against the isolated fungi at the concentration of 200 mg / ml . The minimal inhibitory concentration ( MIC ) of the alcoholic extract of Rosemarinus officinalis was 25 mg / ml . Griseofulvin was more active than Ketoconazole against dermatophytes
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