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دراسات سريرية وجينية في امصال الدم والسائل المنوي للمدخنين وغير المدخنين المصابين بالعقم غير المفسر في محافظة ذي قار - العراق == Biochemical and Genetic Studies of Sera and Semen for Smokers and Non Smokers with Unexplained Male Infertility in Thi - Qar Province/ Iraq
Author name:
وسام ريسان نايف المحسن
Supervisor name:
رائد معلك حنون | ساجد حسن كزار
General topic:
Chemistry
Specific topic:
Clinical Biochemistry
Degree:
Doctorate
University:
University of Thi-Qar - College Of Science - Chemistry Department
Language:
English
University location:
Dhi Qar
Abstract:
This study was conducted on infertile subjects attending to the infertility Unit in Al-Hussein Teaching Hospital in Thi-Qar province south of Iraq, during the period from Aug 2016 to May 2017. The aim of study was to determine the roles of the presence of some biochemical parameters (oxidative stress, antioxidants), antisperm antibodies and polymorphism for glutathione peroxidase (GPx1) and glutathione transferase (GTSp1) in men with unexplained infertility. | Ninety men with unexplained male infertility (UMI) aged (91-91) year and fifty healthy fertile men aged (20-25) year were included in this study as a control group, had no systemic diseases and none were taking an oral antioxidant supplement for three months prior to the study. | The patients and control were divided into two groups (smoker and non smoker). Serum and seminal fluids were collected from each of them, seminogram test (semen analysis) was performed for each participant, and all semen samples were collected following (3-5) days of abstinence. After liquefaction, semen volume, sperm concentration, total sperm count, morphology, motility grades were determined using World Health Organization (WHO) standard procedures. | In this study, there is no significant (P>0.05) between age and body mass index (BMI) of unexplained infertile men compared with age of fertile men (control). The groups of the unexplained infertile males with history of infertility of 3-5 years duration were largest groups who attended medical services. The majority of the unexplained infertile men (90%) complained of primary infertility while the minority of them (10%) complained of secondary infertility. The current study revealed that effect smoking on unexplained infertile group was percentage (44.4%) comparison with control fertile group (36 %). | )II( | The results of semen analysis for the fertile (control) and the unexplained infertile men, revealed no significant differences (P>0.05) in semen volume, liquefaction time, and semen pH between the two groups in (smoker and non smoker). A significant decrease (P<0.05) was seen in the sperm concentration, sperm motility, total progressive sperm (progressive sperm motility and non-progressive sperm motility) and normal sperm morphology in the unexplained infertile group compared with the fertile group (control). While a significant increase (P<0.05) was seen in immotile sperm, sperm agglutination, round cells count and leukocytes in the unexplained infertile group compared with the fertile group (control). | The results showed a significant increase (P<0.05) in serum and seminal plasma Malondialdehyde (MDA) in unexplained infertile (smoker and nonsmoker) groups when compared to fertile (smoker and nonsmoker) groups. The antioxidant such as vitamins (C and E) and glutathione were significantly decreased in serum and seminal plasma for unexplained infertile (smoker and nonsmoker) groups when compared to fertile (smoker and nonsmoker) groups. | A significant increase in serum Ceruloplasmin(Cp) levels in unexplained infertile group comparison with fertile (control) group (P<0.05). Significant differences (P<0.05) in seminal plasma fertile (smoker and non smoker) groups but there was no statistical difference in the Ceruloplasmin level of seminal plasma unexplained infertile between two groups (smoker and non smoker) groups and no significant difference between smoker fertile group with unexplained Infertile (smoker and non smoker) groups. | High prevalence of antisperm antibody (ASA) in seminal plasma for unexplained male infertility a percentage (25.5%) compared with infertility (control) a percentage (14%), and significant differences in ASA seminal plasma | )III( | (Smoker and non smoker) in all groups, which indicator that high in Smoker men compared with non smoker . | In this research found association between the glutathione peroxidase (GPx1) polymorphism and unexplained male infertility, and effect oxidation and antioxidant on these. Therefore, might be a major risk factor of unexplained male infertility in the studied groups, suggested a stronger association between smoking consumption and risk of unexplained male infertility. | The frequency of the glutathione transferase (GSTp1) genotype showed almost no difference between the unexplained infertile men and the fertile controls, therefore, in this case-control study, the GSTp1 variant genotype (Ile/ Ile ,Ile/ Val , Val/ Val) was no significant different in every group of unexplained infertile men compare than in the fertile (control group).This indicating no significant association between GSTp1 and sperm parameters.
