التاثير الوقائي للخلاصة المائية لاوراق نبات الزريج STZ على الكبد واعتلال الخصوبة في ذكور الجرذان المصابة بالسكري بواسطة == Potential Role of Aqueous Extract From Chrozophora Tinctoria Leaves on Liver and Fertility Dysfunction in STZ Induced Diabetic Male Rats
Author name:
منى حسون عبودي
Supervisor name:
محمد عجة عودة
General topic:
Chemistry
Specific topic:
Analytical Biochemistry
Degree:
Doctorate
University:
University of Thi-Qar - College Of Science - Chemistry Department
Language:
English
University location:
Dhi Qar
First pages:
25T1899 - p.pdf
Abstract:
This study was designed to investigate the pharmacological effects on air dried Chrozophora tinctoria (L.) Rafin plant samples as well as the responses of streptozotocin (STZ)-induced diabetic rats for the aqueous Chrozophora tinctoria (L.) Rafin leaves extracts. To achieve this aim, phytochemicals analysis were carried out by various standard assays and then antioxidant compounds contents in the three parts of the plant (leaves, stems, and roots) estimated by common spectroscopic methods. In addition, physiological, biochemical and histological parameters of (STZ) diabetic rats are evaluated too. Rats are distributed into five groups : Control, diabetic rats and three diabetic groups received orally different doses of (50, 70, and 90 mg/kg body weight (BW)) from the target extract for a period of 35 days. When the therapy is ended, blood samples, liver and testis tissues were taken and concentration of glucose, insulin, lipid profile, HbA1C, as well as the activity of ALT, AST, ALP, male sexual hormones were determined as well as liver and testis histology. In general, the results of this study show that the liver and testis tissue were damaged, serum ALP, AST ALT activities, HbA1C, lipid profile, T, LH, FSH, insulin and blood glucose levels were remarkable normalized. It was observed that the BW of diabetic control group is decreased. However the BW elevated slightly in the diabetic treated groups as well as serum insulin and male hormones. In addition, the extract improves the liver function and reduces lesions associated with diabetic state in STZ induced rats. Moreover, the effect of oral administration of Chrozophora tinctoria (L.) Rafin at a dose of 90 mg / kg body weight was more efficient than the 50 and 70 mg/kg body weight. Furthermore, the obtained results related that male reproductive system showed remarkable increased of sperm count, viability and motility after treatment with dose 70 mg/kg of body weight. Histological examination of the testis revealed improvement of spermatogenesis and Leydig cell proliferation with all doses of the extract. However, the histological features of the rats testis received 70 and 90 mg/kg, was found to be similar to the healthy group. The whole results indicated that the extract exhibited protective effect on liver tissues, as well as maintained the damage fertility in diabetic rats. These investigations explain its potentials as an antidiabetic, hepato protective agent, and promising therapeutic to recover male subfertility
