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دراسة وبائية تشخيصية لداء اللشمانيا مع تحديد انماط الطفيل المسبب بواسطة تقنية PCR وتمييز الانواع الناقلة من ذبابة الرمل sand fly في محافظة ديالى == Epidemio Diagnostic Study for Leishmaniasis With Determination of Parasite Strain’s By PCR Technique And Morphologically Differmates The Species of Sand Fly Vector in Diyala Governorate

اسم المؤلف: جابر عودة كاظم
اسم المشرف: توفيق ابراهيم محمد الالوسي | عبد الله حسين عبد الله الجبوري
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية تسليط الضوءعلى انتشار داء اللشمانيا الجلدية والاحشائية المتسبب من قبل الطفيلي Leishmania spp في مناطق مختلفة في محافظة ديالى ودراسة الظروف البيئية المساهمة في انتشار المرض ومقارنة الفحوصات السريرية والمختبرية والمصلية المستخدمة في تش | The aims of present study to illuminate a light on visceral and cutaneous leishminiasis disease caused by leishminia Spp parasite as registered in Diyal Governorate, and study the environment condition which contributed in diffusion the disease and comparison the tests of clinical, Laboratory and serological tests which used in diagnosing with PCR technique test, the study included many steps. 1. The epidemiologically which is main to determine the rates of infection as registered in some district of Diyala province in according to the age, sex, and the month of the year so according to the environmental conditions : - 7200 samples of blood collected from many different ages persons from different districts in Diyala. (3009 males, 4011 females) are examined. The study shows a positive cases of 607 distributed among the districts including (Jalawlaa, Bani Saa'd, Baladruz, Baquba, Alodeim amd DeliAbbas) these register a highest percentage of infection in Jalawlaa sub - district about 12.8% and less percentage of infection are in Baquba about 1.3% whiel is register highest percentage of in infection were (>1 - 5) years about 13.2% and lower percentage infection (<5 - 10) years wuth rate 2.4% so the groups of ages (25 - 30) years didn't appear any infections. according to the gender the males are high from the females whom effected with this disease the number of males (432) case with rate 71, 1%. The number of infected females about 175 cases with rate 28.8%.while in the month of years were the male infection are a highest infection in January - 2012, but did not register any infection in (June and July). The environmental condition infective in prevalence the disease between the people whom resident the houses which breed the animals inside, the number of infections are 245 cases from 607 positive cases with rate 40.3%. This was the low rate 4.9% from the infected people because of rats in their hoses. which didn't kill them. 2. The comparison of diagnostic methods of clinical test, laboratory test and serological test, with the molecular methods from the direction of sensitivity and specificity, The studies results appeared the importance of clinical test subtle in case of a similar cases depend on the clinical symptoms there are a highest cases from clinical test that the children suffering from liver magle their number are 176 cases the rate 80.36%, but the lowest cases of clinical test are 59.19% whom suffered from a skin pallor and black. The laboratory tests appeared the blood picture through testing the 350cases whom suspicion in their infection with leishmaniasis, the number of whom suffering from the lower of WBC 73 with rate 34.2%, while the low cases are 6 with rate 2.82% from whom suffering from hipper ESR, but the serological test was IFAT, Dipsrtick, spotkala - azor, ELISA, KA tex LATEX. Diagnostic infection leishmaniasis a best in diagnostic disease is dipstick test it's sensitivity are 95%, but it's specificity 100% but the lowest is the test of LATEX, it's sensitivity 75% and specificity 80%.3. The Molecular methods : PCR technique test clear recently the new highest sensitivity in diagnosis when the number of the parasite are a little in the blood in the infections peoples whom choose in random way. when testing 100 samples of DNA from the infection with cutaneous and visceral leishmaniasis, the number of infected male are 73, But the number of infected females are 27 with leishmaniasis. and studying the relation between PCR diagnosis according to the sex, age, district and environment condition, so as the recognize between the species of the parasite from the sequence Nitrogen basics with number in each species, (the species are L.donovani, L.infantum, L.tropica, L.major) so recognize the vector insect sand fly Morphology by key classification (ph.alexandri, ph.papatasi Ph.sergentasi, and sqauanlpleuris).

دراسة وبائية على طفيلي المشعرات المهبلية Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقولة جنسيا وتاثير بعض المستخلصات النباتية على نمو الطفيلي في الزجاج في مدينة كركوك == Epidemiological Study on Trichomonas Vaginalis & Some Associated Bacteria That Causing Sexual Transmitted Diseases & Effect of Some Herbal Extraction on The Parasite in Vitro in Kirkuk City

اسم المؤلف: محسن عز الدين سليمان
اسم المشرف: ابراهيم شعبان داوود | محمد عبد العزيز قادر
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: بلغ العدد الكلي للعينات التي جمعت وفحصت للفترة من بداية شهر تموز 2007 الى نهاية شهر مايس 2008 (2345) عينة لكلا الجنسين مــن مستشفيات كركوك للتحري عن وبائية طفيلي المشــــــعرات المهبليـــــــــة Trichomonas vaginalis وبعض الجراثيم المسببة للامراض المنقو | A total (2345) specimens were collected & examined from both sexes, from the beginning of July 2007 till the end of May 2008 attending Kirkuk hospitals for epidemiological study of Trichomonas vaginalis & some sexual transmitted microorganisms. The vaginal examinations were done by gynecologist, two high vaginal swabs were taken from (250) women & a cervical swab from some women with vaginal discharge. General urine examination & blood group with Rh factor were performed for each woman with vaginal discharge. Direct microscopic examination, staining & culture of swabs were performed on different methods for diagnosis of Trichomonas vaginalis, followed to diagnose Candida albicans, Neisseria gonorrhoeae, Chlamydia trachomatis & Treponema pallidum as well as some other bacterial groups. The rate of Trichomonas vaginalis was (2.8%) & Neisseria gonorrhoeae (0.8%), while no Chlamydia trachomatis & Treponema pallidum recorded in this study. The highest rate of Trichomonas vaginalis was among the age group between 15 - 29 years (3.6%), the rate of Trichomonas vaginalis infection was higher among illiterate (5.7%) than educated ones. The highest rate of Trichomonas vaginalis was recorded among those with blood group O & Rh (+ve) (3.8%). The same result was found regarding other microorganisms in both sexes. Statistically the results were not significant.The rate of infections in females was higher than males in all infection except Neisseria gonorrhoeae was recorded highest rate in males(35%). The percentage of mixed infections with Trichomonas vaginalis was Candida albicans (1.6%) & B - hemolytic streptococcus (0.4%). The maximum infections rate in vaginal swabs were Staphylococcus aureus (42.3%) & lowest rate of Neisseria gonorrhoeae (2%). Regarding the distribution of microorganisms isolated from urine samples of females suffering from vaginal discharges, the highest rate of infection was Eschirechia coli (22.2%) & lowest rate of Neisseria gonorrhoeae (2%) respectively. The rate of isolated microorganisms from urethral discharge of males were Neisseria gonorrhoeae (35%) & Escherichia coli (5%). Antibiotics Ciprofloxacin & Amikacin showed good inhibition activity against all pathogenic microorganisms. Regarding the effect of different concentration of metronidazole & medical herbs to the Trichomonas vaginalis invitro, metronidazole showed clear effective inhibition at concentration (1.25%) during 24 hours. The inhibitory effect of Zea mays (both alcoholic & watery extractions) was greater than Apium graveolens & Foeniculum vulgare. At concentration (2.5% & 5%) of Zea mays in both extractions was almost identical to metronidazole. Six solvents were used by thin layer chromatography (TLC) in order to separate alcoholic & watery extraction of medical herbs, only three of them A, B & F gave good results in separating herbal extraction with differing in its component. By measuring relative flow rate (Rƒ) of all compounds that separated and determind. The chemical compounds of each herbal extract were established & included alkaloids, flavonieds, amines, pigments, oils, carbohydrates, phenols, purines & tanines.

عزل وتشخيص بعض انواع الجراثيم المسببة لالتهابات الزائدة الدودية في مدينة تكريت == Isolation And Identification of Some Baceterial Speices Which Caused Appendices Infections in Tikrit City

اسم المؤلف: عبد عبد الله محمد الجبوري
اسم المشرف: اميرة محمود محمد الراوي | علي صالح حسين الجبوري
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تضمنت الدراسة جمع 75 عينة من الزائدة الدودية (Appendices) مباشرة بعد استئصالها من المرضى الراقدين في مستشفى تكريت التعليمي ومن كلا الجنسين، الذين خضعوا لعملية استئصالها وللمدة من اب عام 2008 ولغاية نيسان 2009. عزلت الجراثيم من العينات باستخدام الاوساط الز

تشخيص بعض النواتج الحيوية لانواع من السيانوبكتريا المعزولة من مياه وترب مدينة تكريت ودراسة فعاليتها على بعض انواع البكتريا المرضية والحيوانات المختبرية == Identification of Some Bioproducts From Some Species of Cyanobacteria Isolated From Water And Soil From Tikrit City And Study Their Biological Effects on Some Pathogenic Bacteria And Laboratory Animals

اسم المؤلف: ايمن عوني سليم جاسم
اسم المشرف: حميد سلمان خميس المهداوي | احسان محمود عبد الله
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - البكتريا
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية عزل وتشخيص (19) نوع من السيانوبكتريا من عينات مياه وتربة طينية ومن الصخور من (5) خمسة مواقع في مدينة تكريت ضمن محافظة صلاح الدين، وقد تم اختيار ثلاثة انواع من هذه السيانوبكتريا وهي : Anabaena oryzae, Nostoc linckia, Anabaena laxa اعتم | The current study included Isolation and Identification of (19) species of cyanobacteria from water, clay soil and rocks samples, collected from (5) sites in Tikrit, salah Al - den province. Three species were chosen each species represent the environment which collected from it. These species were Anabaena oryzae, Nostoc linckia, Anabaena laxa. The effects of some environment factors on growth and biological products quantity has been studied in the three species above. The effects of the temperature showed that 26°C was the best for growth and biological products quantity than 16°C and 36°C. While the pH effects showed that pH 7.6 was the best for growth and biological products quantity than pH 6.6, 8.6. Three light intensities were used 1250, 2500, 5000 lux were used. The results showed that the light intensity of 2500 lux was the best for daily growth and quantity of extracted biological products. While the study of the effects of addition of macronutrient and micronutrient to Asm - 1 media which used for culturing of the three species of cyanobacteria by replacement of NaCl by NaNO3 in the medium, showed that there is an increase in daily growth and quantity of extracts of biological extracts, also the addition of FeSO4.7H2O and NaNO3 together showed further increase of daily growth and biological extracts quantity. The biological products has been identificated by HPLC - MS, IR and NMR. The results showed the presence of the neurotoxin Anatoxin - a and the hepatic toxin Microcystin in its various forms MCYST - LAba, MCYST - YM(O), [D - Asp3, Dha7]MCYST - LR and MCYST - YA in the biological products of Anabaena oryzae. The results also showed the presence of the hepatic toxin MCYST - YM(O) and [D - Asp3, Dha7]MCYST - LR in the extracts of the two spices of cyanobacteria Nostoc linckia and Anabaena laxa.The results also showed that the Muller Hinton Agar media is better than the Blood Agar media in testing the activity of these extracts in inhibition of the pathogenic bacteria, the method of holes is better than saturated discs, and the solvent DMSO (Di - Methyl Sulpho Oxide) is the better in testing of biological activities, while the filtrate of cyanobacteria did not shown any activity for inhibition of the pathogenic bacteria which include : Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Klebsiella pneumonia, Proteus Vulgaris, Staphylococcus aureus. The results showed the high capability of inhibition in concentrations of 1000, 2000, 3000 µg/ml of the extracts of the cyanobacteria Anabaena oryzae, Nostoc linckia and Anabaena laxa on the above pathogenic bacteria and some times more efficient than antibiotics used for comparison, and the concentration 3000 µg/ml was the most effective.The effects of biological extracts of the three species in concentrations of (1000, 2000, 3000) µg/kg of body weight on tested albino mice, using injection and feeding methods, was studied. The results showed poisoning nervous symptom for the first few hours, while the results showed hepatic poisoning symptom on the dissecting animals. The anatomic symptoms included changes the colour of the liver to darkening and appearance of necrosis, in addition to becoming lobbed and swollen some times with bleeding.While the poisoning effects on dissecting tissues of livers of the animals showed a simple swollen and some times assured in all of the liver accompanying with increase in growth and cell division, as well as increases in lymphatic cells inside the tissue and in general in liver tissue. The lymphocyte seemed bigger than for normal cell with multi - nuclei in white blood and increase in cell size and destruction of liver tissue. Nostoc linckia was the greater urging for cell division consequently probably more induce of cancer in the liver of the animals, while the biological product of Anabaena laxa showed more poisonous for liver cell than the biological products of Anabaena oryzae, the least effective in cytotoxic and urging cancer cell in liver of the animals.

تحديد مقاومة بعض الجراثيم المعزولة من اخماج في مستشفى تكريت التعليمي للمضادات الحيوية وعلاقتها بالبلازميدات == Determination The Antibiotic Resistance of The Some Isolated Bacteria From Tikrit Teaching Hospital Infections And Their Relationship With Plasmids

اسم المؤلف: شيماء عبد محمد علي
اسم المشرف: وعد محمود رؤوف | خالد عمر محمد علي
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: جمعت (140) عينة من المرضى الراقدين لثلاثة ايام فاكثر في مستشفى تكريت التعليمي للمدة من 1 - 8 - 2009 ولغاية 1 - 12 - 2009 بهدف تحديد العزلات الجرثومية الاكثر شيوعا ضمن اخماج مستشفى تكريت التعليمي ومقاومة هذه العزلات للمضادات الحيوية ودراسة العلاقة بين مقاو | One hundred fourti samples were collected from hospitalized patients for three days or more in the Tikrit Teaching Hospital during the period from 1/8/2009 until 1/12/2009 to determine the most commonly isolated bacterial from nosocomial infections in Tikrit Teaching Hospital and their resistance to antibiotics and to study the relationship between antibiotics resistance and plasmid content in the most commonly isolated bacteria and to evaluate the prevalence of these plasmids in the hospital environment. These samples include : (75) Urine, (28) stool, (28) of wound swabs and (9) sputum samples.The incidence of nosocomial infection was (45.7%) of them, urinary tract infections were (50.7%) from (75) samples, followed by wound infections (42.9%) from (28) samples, gastrointestinal infections (39.3%) from (28) samples then pneumonia (33.3%) from (9) samples the nosocomial infections were more common in females and age group 51 - 89 years. Different bacterial species were isolated in this study and the results showed that the most commonly isolated type was Citrobacter koseri in a proporation (21%) of urinary tract infections, followed by the following bacterial species : Escherichia coli (15.7%), Staphylococcus schleiferi (10.5%) , Staphylococcus epidermidis (7, 8%), Proteus vulgaris, Staphylococcus saprophytica, Klebsiella ozaenae, Klebsiella ornithindytica in aproportion (5.2%) for each, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis in aproportion (2.6%) for each.Citro. koseri were also the most common in wound infections (25%), followed by the following bacterial species : E.coli (16.6%), P.mirabilis (16.6%), Staph.schleiferi, P.vulgaris, Prov.stuartii and Prov.rettgeri in aproportion (8.3%) for each. Citro.koseri were also the most common in the gastrointestinal tract infections in a proporation (36.3%), followed by E.coli (18.1%), S.flexneri (18.1%), P.mirabilisc, Prov.stuartii, Prov.alcalifaciens in a proporation (9%) for each. . While Strept.pneumoniae was the most common(66.7%) in penemonia cases followed by K.pneumoniae (33.3%).The isolated bacteria were tested for antibiotics susceptibility and it has been found that most of these isolates showed high resistance to the studied antibiotics other than Prov. Stuartti was sensitive to all antibiotics except of Rifampicin and Tetracycline. As well as bacterial species belonging to the genus Staphylococcus were sensitive to Amikacin, Staph.aureus was also sensitive to Nitrofurantoin while Staph.warneri was also sensitive to Ciprofloxacin and Nitrofurantoin also Enterobacter aerogenes was resistant to all antibiotics except for Ciprofloxacin and Nitrofuratoin. Eleven of most commonly isolated bacteria which were multidrug resistant were chosen for the curing experiments using SDS to study the relationship of their plasmids with resistant to antibiotics. The results showed that the cured cells of E.coli lost their resistance to Tetracycline Ciprofloxacin and Cefotaxime which Citro.koseri lost their resistance to Tetracycline while K. ornithinolytica lost resistance to both antibiotics Tetracycline and Ciprofloxacin either P.mirabilis lost resistance only to tetracyclin as well as Strept.pneumoniae lost their resistance to Cefotaxime, tracycline and Ampicillin. Which may mean that the genes responsible for resistance to these antibiotics are located on plasmids as proven by the results of electrophoresis.Electrophoresis results also showed that all studied bacterial isolates had the same plasmid profile which may regarded as an epidemiological pointer that plasmids are conjugative which have the ability to transfer antibiotic resistance to other bacterial strains of the same species or to other different bacterial species.

التحديد الجزيئي لبعض عوامل الضراوة في بكتريا Klebsiella spp المعزولة من مصادر سريرية مختلفة == Molecular Identification of Some Virulence Factors In The Bacterium Klebsiella Spp Isolated From Different Clinical Sources

اسم المؤلف: فرقد حسن فليح
اسم المشرف: بهيجة عبيس حمود الخالدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: جمعت (150) عينة من المرضى المراجعين والراقدين في مستشفى الديوانية التعليمي والذين يعانون من اصابات مختلفة وللفترة من 1/كانون الاول/2013 ولغاية 1/نيسان /2014 وتضمنت هذه العينات ماياتي : (50) عينة حروق وجروح، (50) عينة ادرار و(50)عينة قشع من المرضى المراجعين | One hundred fifty samples were collected from patients admitted in Al - Diwaniyah Teaching Hospital and suffering from various injuries and for the period from 1 / December / 2013 and up to 1 / April / 2014 these samples in clude : (50) sample burns and wounds, (50) urine and (50) sputum.The results showed that 90 samples (60%) of the total number gave is positive result for the growth.the bacterial isolates were distributed among 35 samples (70%) of burns and wounds, (33) the sample (66%) from urine and (22) sample (44%) from sputum After biochemical and cultural test found that 99 isolate belonging to Different Genus and species of bacteria these isolates include 40 isolate (44.44%) bound and wound isolates Staphylococcus sp was the predoniant in these samples by rate (42.5%), 36 isolate(40%) E.coli was most isolate by rate 50% in urine sample and 23 (25.5%) Klebsiella pneumonia was the most common isolate by rate (78.26%) in these putum.. Sensitivity test results of antibiotics showed that most of the isolates have high resistance against antibiotics used in the study and the most isolates possess multiple resistance (Cross resistance) as shown resistance to most of antibiotics that used in these study. All asolates (% 100) were resistant to nitrofurantin and Tetracycline while(95%)is resistant to ampiciline (90%) of isolate were resistant to thired generation of cephalosporins (ciprofloxacin and ceftazidime) and (%80) of isolate wereresistant to gentamicin and impeneme, finely (%80)of isolate were resistant to azetroname..The results of the molecular detection of genes uge and mag A gene in Klebsiella sp in the 16 (50%) of the isolates had mag A as the 8 (44.44%) of the isolates bacteria K.pneumonia carrier of mag A and 8 isolates of type K.oxytoca had of the gene above the gene uge was his appearance in the proportion of isolates (46.87%) as it appeared in 9 (50%) of the isolates K.pneumonia and 6 isolates (42.87%) of the isolates k.oxytoca..There were relationship between resistance of bacteria to antibiotics and possession of these genes

انتشار المقاومة للمضادات الحيوية وبعض عوامل الضراوة في بكتريا Pseudomonas aeruginosa المعزولة من مستشفيات في النجف المعزولة من مستشفيات في النجف == Molecular Study To Some of Pseudomonas Aeruginosa Virulence Factors Isolated From Clinical And Environmental Isolates

اسم المؤلف: ذو الفقار حيدر حسن
اسم المشرف: ماجدة مالك متعب
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية لغرض التحري عن وجود الجينات الكروموسومية المسؤولة عن بعض عوامل الضراوة المهمة لجنس Pseudomonas aeruginosa كانتاجها للسم الخارجي A والالجنيت اضافة الى الجينات الكروموسومية المسببة لمقاومة الكوينولونات في العزلات السريرية والبيئية لبكت | This study include investigate the presence of chromosomal quinolone resistance genes and virulence genes(toxA and algD) in clinical and environmental isolates from Al - Sadr Medical city.A total of 110 samples were collected from different clinical sources(burns, wounds, and urine), and 50 from the hospital environment.the samples were collected during 10, Oct, 2012 to 2, Feb, 2013. A total number of Pseudomonas aeruginosa isolates accounted for 89 of 160 samples.The distribution of these isolates were 28 from Burns, 14 wounds, 15 urine, and 32 from hospital environments. Identification of isolates has been accomplished depending on phenotypic diagnostic methods and PCR technique.our study proved that PCR technique depending on oprI and oprL genes was a rapid method for identification of non - pigmented isolates. A total of 20 antibiotics resistant isolates were chosen for Molecular studies. The result reveling that 100% of isolates had a chromosomal mutation in the QRDRs of genes (gyrA, gyrB, parC, and parE). High percentage of isolates(45%) carried mutated genes gyrA and parC, followed by 15% of isolates were carried either mutated gene gyrA alone or three mutated genes gyrA, parC, and parE and and 10%of isolates were carried three muted genes gyrA, gyrB, and parC, while gene that encoding to parC mutation were detected in 5% of isolates. Our study is the first study about detection of Quinolone Resistance Determining Regions in P.aeruginosa in Iraq. The results showed the the presence of mexAB - oprM multidrugs efflux system expression in 100%(20) isolates. while the phenotypic test depending on MIC values for ciprofloxacin alone in the presence of efflux inhibitor showed that only 15%(3) isolates had the active efflux pump.

التعبير الجيني للمورث Erg11 وعلاقته بالمقاومة وظاهرة رجوع النمو لبعض عزلات خميرة المبيضات البيضاء المختبرة تجاه مضادات الازول الفطرية == Gene Expression of Erg11 And It’s Relation With Resistance And Trailing Growth Phenomena of Some Isolates of Candida Albicans Tested Against Azole Antifungals

اسم المؤلف: حيدر عبد الحسين عباس
اسم المشرف: عدنان حمد عبيد الحمداني
الموضوع العام: علوم الحياة
السنة: 2013
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية الى الكشف عن دور المورث Erg11 والتعبير الجيني النسبي له في مقاومة خميرة المبيضات البيضاء C.albicans وعلاقته بنشوء عزلات مقاومة او ظاهرة رجوع النمو Trailing growth لهذه الخميرة عند اختبار حساسيتها الدوائية تجاه بعض مضادات الازول (الفلو | The current study aimed to detect the role of Erg11 gene and its relative expression in the resistance of Candida albicans, and its relationship with the emergence of resistace or Trailing growth isolates when tested its susceptibility against azoles agenst (fluconazole, ketoconazole). A total of 120 specimens were collected from patients of both gender with different ages whom infected with oral thrush, uro - genital and Gastrointestinal candidiasis and admitted the General Teaching Hospital and materinaty and children Teaching Hospital in AL - Diwaniyah city during the period from December/2012 to February / 2013. The isolation and identification of yeast isolates were done based on their morphological, Cultural and biochemical tests in addition to use the confirmatory systems such as the growth on Chrom Agar candida, Api Candida and Tobacco Agar medium to distinguish C.albicans from other yeast spp. Also, some virulence factors of C.albicans such germ tube formation, chlamydospores production, growth at of 45 c and resistance to cycloheximide were detected. The antibiotics sensitivity test was used in two method (disk diffusion & dilution) to determine the sensitive, trailing growth, resistance isolates and minimal inhibitory concentration (MICs) toward azole agents. The qRT - PCR was used to detect the relative quantitative of Erg11 gene expression and its relationship with resistance to azole and emergence of trailing growth phenomena in the presence of Fluconazoles and ketoconazole. The isolation and identification results revealed that the isolation percent of C.albicans was 47.05 % incomparison with other Candida species that were 15.3%, 11.76 %, 8.23 % and 5.9 % for C.krusi, C.dubliniensis, C.tropicalis, C. parapsilasis and C.glabrata , respectively. The antibiotic susceptibity tests of C.albicans showed the presence of resistance precent (38.89%) to fluconazole and the most of sensitive isolates revealed a trailing growth phenomena in the zone of growth inhibition of floconazole in a ratio 55.56%. While the resistance percent to ketoconazole was 27.78 % and the sensitive isolates showed the trailing growth in a ratio (38.89%). The statistical analysis showed a significant differences (P <0.05) among tested treatments. The MICs values of fluconazole against C.albicans isolates were 8, 16, and 64 Mg/ml for sensitive isolates and were 0.125 and 0.5 Mg /ml for resistance isolates and the trailing growth phenomena occurred at MICs value ? 8 Mg/ml within 24 hours of incubation while the values became ? 64 Mg/ml after 48 hours of incubation.There was a significant differences (p< 0.05) among tested treatments isolates incubated at 24 hours while there was no significant differences (P>0.05) at 48 hours of incubation. In respect with Ketoconazole, the resistance of C.albicans was increased after 48 hours of incubation at MICs values 0.125, 0.25, 0.5 and 1 Mg /ml while its was sensitive at values 4, 8 and 16 Mg /ml and the trailing growth phenomena occurred when the MICs ? 2 Mg/ml after 24 hrs of incubation, while it reached to ? 8 Mg/ml after 48 hrs of incubation. There was a significant differences (p< 0.05) among tested treatments incubated after 24 hours while there was no significant differences (P >0.05) among treatments after incubation 48 hours. The results of relative quantitative gene expression for both genes revealed a significant differences (p< 0.05) in gene expression levels through the occurrence of increased in the transcription of mRNA quantity among tested treatments.The up - regulation of Erg11 and fold change in mRNA transcription was 2.498 fold compared with 1.0 for control treatment after 24 hrs of incubation in the presence of antifungal, while the increasment was 12.606 fold after 48 hrs of incubation of isolates with antifungals.

التمنيع والتحدي لبكتريا Citrobacter freundii فاقدة الجدار المسببة لخمج السبيل البولي == Immunization And Challenge For Walled Citrobacter Freundii And Walled Defective That Causing Urinary Tract Infection

اسم المؤلف: عبد الرسول عويد عداي الشبيب
اسم المشرف: قاسم نجم ثويني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: Cryptic bacterial infection has been studied in urinary system to Human, where 50 underwent urine samples from patient mostly based consulting auditors Hilla public Education Hospital for the period from November 2012 until Feb. 2013. Patients ranged in age from 20 - 50 years males and females bacterial infection rate was 70% distributed between the cell wall defective bacteria had 38% and 32% cell - wall bacteria and the results showed 30% as negative cases. Amicroscopic examination of the urine sludge Showed clear sovereignty of non - specialized immune response type of mixed cell 34% followed by mononuclear cell 30%. Samples were cultured on routine media (Blood agar , Macchonky agar) and Modified media (Variant agar, Variant broth). Out put diagnosed by bacterial system diagnostics, phen - otypic, microscopically and biochemistry by using the special test for microorganism diagnosis and using API 20 E and the use VITEK 2 to confirm some samples results. Characterized by colonies of cell wall defective bacteria grow on variant agar it resembles fried egg which could seen after staining Deinse stain. Citrobacter freundii recorded 12%, the highest rate among cellwall defective isolates. Sensitivity test for bacterial isolates were screened from U.T.I both two type (CWB & CWDB) showed the highest rate of sensitive to Impinem 100% represented CWDB, as well as the highest rate of resistance to Ampicillin 100% represented by the same group of isolates. experimental study with urinary tract infection by injecting bacterial stuck of Citrobacter freundii to the three groups of rabbits : first group ( 3 Rabbits ) : immunized with viable surface antigen extracted from CWDB, second group : ( 3 Rabbits ) immunized with heat killed surface antigen extracted from CWB, the third group (4 Rabbits) represented control group it been treated with sterile normal saline. the injection process through urethra, and then underwent a histological study, was histological damage to the members of the animal - derived non - immunized (control group) seemed clear was the appearance of symptoms of the total cellular infiltration and disruption of the mucous layer and epithelial and other symptoms. The immunized animals have shown their ability to defy injury. It is the study of serological reactions to bacterial antigens Citrobacter freundii immunized with vaccines for rabbits given high caliber agglutination with its specialized vaccines and low with adversity and that the absorption and the corresponding and variant antigen does not remove the agglutination effectiveness with immune sera prepared suggesting that the absorption is incomplete and that the difference between the wall and a wall defective in terms of antigenic is quantitative and qualitative.

تاثير انزيم SNase المنقى من العنقودي في الغشاء الحياتي للايشيركية القولونية والكليبسيلا الرئوية == Impact of Snase Purified From Staphylococcus Aureus On Biofilm of Klebsiella Pneumoniae And Escherichia Coli

اسم المؤلف: هند تحسين ابراهيم
اسم المشرف: حارث جبار فهد المذخوري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: SDS - PAGE showed that a single sharp band with an approximately 16.8 KDa molecular weight has been seen. A matter indicates that the enzyme is consistently pure. PCR technique was applied to approve the existence of nuc gene in S.aureus Nevertheless, only those which depicted positive results on DNase agar harboured nuc gene, as it is specified by single band appearance of nuc at assumed molecular weight (ca. 255 pb) of marker that being used. The current study findings highlighted the participation of SNase purified from S. aureus in significant (P< 0.05) preventing biofilm formation by E. coli and K. pneumoniae compared with untreated controls. Evidently, the inhibitory effect of SNase on biofilm formation is undoubtedly perceived, indicating the degradation of the structural major component of biofilm formation (i.e. extracellular DNA). Results revealed that SNase was able to significantly (P< 0.05) reduce the number of the uropathogens; E. coli and K. pneumoniae attached to the uroepithelial cells. 1 Chapter one : Introduction and literature Review 1. Introduction and Literature Review 1.1. Introduction Staphylococcus aureus is a persistent human pathogen that is responsible for a range of diseases that vary widely in clinical presentation and severity. The pathogenesis of S. aureus infection is a complex process involving a diverse array of secreted and surface - associated virulence determinants that are coordinately expressed at different stages of infection (Loughman et al., 2009). Escherichia coli is a genetically diverse species that causes a variety of infections which fulfill many or all of the proposed criteria for biofilmassociated infections (Kaper et al., 2004). Klebsiella pneumoniae is an opportunistic pathogen responsible for a wide range of nosocomial infections. One important factor associated with virulence in K. pneumoniae is its capacity to adhere to surfaces and form biofilms (Cruz et al., 2012). Bacteria often exist as sessile communities called biofilms which are exquisite structures caused by a genetically programmed developmental process. It is estimated that biofilms are involved in 65% of human bacterial infections, since cells in biofilms are 1000 times more resistant than cells in the planktonic state, making medical treatments fail (Shal? et al., 2011). Extracellular DNA (eDNA) plays a significant role in biofilm formation, as revealed by studies in several bacteria including E. coli (Nakao, 2012) and K. pneumoniae (Whitchurch et al., 2002); however, there is no definite proof on a cause - and - effect relationship between DNA release and biofilm formation (Beenken et al., 2012) or becomes a key component of the macromolecular scaffold in many different biofilms (Jakubovics et al., 2013). In rare cases eDNA has been shown to inhibit bacterial settlement. Therefore, it is possible that nuclease mediated eDNA degradation would therefore promote adhesion. Consequently, it is not clear whether microbial nucleases contribute to the gross biofilm structure in clinically relevant situations (Sheilds et al., 2013). 2 Chapter one : Introduction and literature Review All previous studies used both commercial bovine and recombinant human DNaseI in the disruption of medically important biofilms; whilst, extracellular nuclease of S. aureus (SNase) was used against biofilm of S. aureus themselves (Benenken et al., 2012b ; Kiedrowski et al., 2014). Thus, to date, the role of S. aureus exonucleases in biofilm of other bacteria remains unclear. However, in the present study SNase is used against biofilm of other bacterial species (viz. K. pneumoniae and E. coli). To address this, the following steps were undertaken : 1 - Investigating the negative impact of SNase extracted from S. aureus on K. pneumoniae and E.coli biofilm formation. 2 - Studying the preventive activity of SNase on the adherence of K. pneumoniae and E. coli on uroepithelial cell.

دراسة وبائية وسريرية لمرض الجيارديا Giardiasis في اطفال مدينة الشرقاط وضواحيها == Parasitic Study of Giardiasise Among Children in Sharqat City

اسم المؤلف: علي حمد طلال موسى الجبوري
اسم المشرف: حسين ساهر اسود العبيدي | عبد الله حسين عبد الله الجبوري
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لبيان تاثير طفيلي الجيارديا المعوي على صحة الاطفال في قضاء الشرقاط لـ(250) طفل، تم اختيارهم بصورة عشوائية، اذ تراوحت اعمارهم من شهر - 15 سنة وللمدة ما بين شهر تشرين الاول 2006 لغاية شهر تشرين الاول 2007، تم فحص عينات الغائط من كل طفل بال

دراسة بيئية ونوعية لمشروع تصفية ماء الكرخ في الطارمية == An Ecological And Qualitativ Study for Al - Karkh Drinking Water Plane At Al - Tarmia

اسم المؤلف: احمد توفيق احمد المشهداني
اسم المشرف: رياض عباس عبد الجبار
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: نظرا لما لمياه الشرب من اهمية كبيرة في حياة الانسان وضرورة ان تكون هذه المياه ضمن مواصفات ومعايير خاصة, فضلا عن ظهور العديد من مشاكل تلوث مياه الشرب في مختلف مدن العراق. دعت الحاجة لاجراء هذه الدراسة لبيان مدى صلاحية مياه الشرب المنتجة من مشروع تصفية ماء | Owing to the drinking water of great importance in human life and it is important to be under the specifications and standards, as well as the emergence of many of the problems of contamination of drinking water in various cities of Iraq. Needed to conduct this study to demonstrate the viability of drinking water produced from the project filter water Karkh (which is the largest project the liquidation of water in the Middle East, it is feeds many of the cities of Baghdad, on the side of Karkh) and compare the specifications of Iraq and the global standard for drinking water in order to investigate the potential weaknesses in the project and study the possibility of the development of such large projects as a first step to improving the situation of drinking water in Iraq. So were the tests, physical, chemical and biological common to drinking water in the project and divided into four main stations, namely, (station socket raw water, sedimentation basins secondary sand filters, pumps, distribution of drinking water), including nine stations secondary starting of the month of October - 2010 to July - 2011.The results showed high values of turbidity, and electrical condactivety and pH, and dissolved oxygen, and biological dimond of oxygen, and alkalinity, and total hardness, calcium hardness, magnesium hardness, chloride, phosphate, nitrite, silica, fluoride, sodium, potassium, and heavy metals, namely, (iron, copper and cadmium, nickel, lead, chromium and zinc), and the total numbers of bacteria, and total coliform bacteria, the fecal coliform bacteria and E. Coli in drinking water for most of the stations in the Alsta spring and low concentrations in summer and autumn.Values for water temperatures between (4 - 37.5) c, as recorded differences significant between the months of winter and spring, summer and autumn, and the values of brownish discharge ranged between (250 - 0.3) NTU as it showed the differences of the moral high values of turbidity in the winter and fall in the summer and fall in each stage of the treatmant with it, and the values of electrical conductivity was between (520 - 895) Micro Siemens / cm 3. The water Alnehrualcherb in the project was registered as a weak base pH values between (9.7 to 1.7), and it was well ventilated, as the oxygen values were high in the winter months registered clear monthly variation ranging between (5.14 - 2.3) mg / L, either values biologicaldimond of oxygen were values between (4.4 - ND) mg / L and found that basal total stations ranged from every stage of the liquidation potential in increasing concentrations of dissolved oxygen, between (160 - 100) mg / L, and found that drinking water hardness too, as that the values of total hardness ranged between ( - 215 335) mg / L as suited their values directly proportional to both the calcium and magnesium hardness ranging between (197 - 139) and (31.472 - 16.128) mg / L, respectively, while for the values of the chloride ranged between (69 - 25) mg / l, while the fluoride has recorded values ranged between (15 - 0.05) mg / L, with values ranging between sulfate (169.2 - 90) mg / L, free chlorine residual values ranged between (4.18 - 1.1) mg / L, and materials for suspended solids ranged values between (84 - ND) mg / L proven contribution stages of liquidation, all in the rates of removal of different, either TDS fluctuated values between (545 - 315) mg / L, with log Aluminum values ranged between (0.98 - 0.01) mg / L, but for the potassium values ranged between (3.2 - 2.15) mg / L, and sodium values ranging between (6.31 to 3.21) mg / liter.As for the concentrations of heavy metals did not detect any significant concentration of heavy metals measured except for iron ranging between concentrations (9.2 to 8.1) mg / L, so after the industrial plants that contain the droppings of these metals. The nutrient values were recorded between nitrite (0.024 - 0.001) mg / Trosgelt nitrate values between (1.7 - 0.16) mg / l, recorded between ammonia (0.54 - ND) mg / L, placed at either phosphate values ranged between (0.26 - ND) mg phosphorus atom - phosphate / L, while for the values of silica fluctuated between values (5.12 to 5.1) mg atom of silicon - silica / liter. As for the study of signs bacteriological ranged the total numbers of bacteria in air through (24) hours between (555 - 0) × 10 cells / 1 ml, and ranged in numbers for a period of (48) hours between (1186 - 0) 10 × cells / 1 ml, while for the numbers of total coliform bacteria fluctuated between (1700 - 0) cells / 100 liters, and fecal coliform bacteria, they ranged (1100 - 0) cells / 100 ml, and the E.coli bacteria, the numbers fluctuated between (1100 - 0) cells / 100 ml. For to match the drinking waterproduced from the projects liquefaction research found matching the values and concentrations of brownish, and connectivity electrical and pH, and dissolved oxygen, and the requirement is vital for oxygen, and basic college, and chloride, and ten college, and magnesium, nitrite, phosphate, and the total numbers of bacteria, and the numbers of total coliform bacteria, and fecal coliform bacteria and E Coli, and silica with the standard specifications for drinking water the Iraqi and global at all stations.While the study results show non - conformity of calcium concentrations in this study with the standard specifications for drinking water of Iraq and the world.

عزل وتشخيص البكتيريا Bacillus thuringiensis ودراسة تاثيرها في بعض المقاييس الحياتية لذبابة ثمار القرعيات (Diptera : Tephritidae) Dacus ciliatus == Isolation And Identification of Bacillus Thuringiensis And Studying Its Effects On Some Biological Parameters For Dacus Ciliatus (Diptera : Tephritidae)

اسم المؤلف: ادريس صالح جليل
اسم المشرف: عدنان نعمة عبد الرضا العزاوي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة عزل البكتيريا Bacillus thuringiensis من مصادر بيئية مختلفة في محافظة ديالى وتشخيصها بالطرق الكيموحيويه والوراثية والجزئية، اذ جمعت 51 عينة من ترب مختلفة وهي(زراعية وحديقة وغير زراعية) وكذلك من الماء، من خمسة اقضية في محافظة ديالى (بعقوبة | This study included the isolation of pathogenic bacteria Bacillus thuringiensis from different environmental sources in Diyala province, diagnosis biochemical and genetic partial ways, Mar 50 samples collected from different soils, namely, (agricultural soils and garden soils and non - agricultural soils) as well as from the water, from five districts in the province of Diyala (Baquba, Khalis, Muqdadiyah, Khanaqin, Balad Ruz) for the period from 1 \ 9 \ 2014 to 20 \ 11 \ 2014. Showed the results of biochemical diagnosis and the presence of four samples and by percent 8% It was of a negative for transplantation bacterial and 46 sample growth by percent 92% It was of a growth positive for transplantation bacterial and found that 41 samples of which by percent 82% belong to the genus Bacillus and the species of Bacillus thuringiensis and the percentage of bacteria isolated of agricultural soils 94% and soils garden 93% and water 75%, and soils non - agricultural 44%. The pathogenic bacteria Bacillus thuringiensis of positive bacteria a gram dye and spore forming and The most of the natural habitat this bacterium is soil and it is capable of producing the diversified varieties of crystalline proteins with insecticide property. All isolates were tested for this bacteria that which were able growth on the media selective Acetate Sodium Selective and the production of insecticidal crystalline proteins (ICP) When staining by dye Coomassie brilliant blue (CBB) to conduct genetic partial diagnosis using several extraction (Mini gDNA Bacteria kit) processed by Bioneer company was the purity of DNA extracted ranging between (20.1 - 1.22) for all selected isolates, have been diagnosed with the bacteria at the level of genus and species Using the gene 16S rDNA with a molecular weight of 380 base pairs where the results showed polymerase chain reaction (PCR) that all isolates contain this gene and the same molecular weight which is 380 base pairs, also was diagnosed isolates containing the gene component crystalline proteins which cry a e molecular weight 200 base pairs using primer a specialist for this gene in polymerase chain reaction showed the results of gene doubled that all isolates contained the gene and the same molecular weight of 200 base pairs. After making sure of pathogenic bacteria as belonging to the genus Bacillus and species Bacillus thuringiensis according to their chemical diagnostics biochemical and diagnostic genetic partial, attended different concentrations They (1, 2, 3, 4, 5) g/L of thebio preparation for these bacteria to study their impact on the different roles of Cucurbits fruit fly Daus ciliatus. Has been tested the effect of different concentrations of bio - preparation of these bacteria isolated from soil and water on the roles of this insect which eggs and larvae and pupa and AdultsThe results of test the effect of bio - preparation of these bacteria on the eggs of age (1 - 2) Since the highest rate of hatchling (3.2) and by (89%) at a concentration of 1 g / l of bio - preparation and lowest hatchling (0.6) and by (19%) at a concentration of 5 g / L of bio - preparation comparison with control (3.2) and by (96%) for the isolated bacteria from soil Since the highest rate of hatchling (3.1) and by (92%) at a concentration 1 g / L of bio - preparation and lowest hatchling (2.1) and by (62%) at a concentration of 5, 4 g / L of bio - preparation comparison with control (3.2) and by (96%) for isolated bacteria from water, and on The last larval phase, The highest the value of the rate of highest (2.0) and by (99%) at a concentration of 5 g / L of bio - preparation and The less the value of the rate of loss (0.5) and by (26%) at 1g / L of bio - preparation concentration compared with the control treatment (0.07) and by (%3) for isolated bacteria from soil.reached its highest value the loss rate (1.2) and by (57%) at a concentration of 5 g / L of bio - preparation and less the value of the loss rate (1.0) and by (6%) at a concentration 1 g / L of bio - preparation comparison with control (0.07) and by (3%) for the bacteria isolated from water and on pupa age(6 - 7) day, reaching the highest rate of emergence of pupa (3.2) and by (92%) at a concentration of 1 g / L of bio - preparation and less the emergence of pupa rate (0.4) and (13%) at a concentration of 5 g /L of bio - preparation comparison with control (3.2) and by 96% for bacteria isolated from soil reaching the top of the emergence of pupa rate (3.1) and by (92%) at a concentration of 1 g / L of bio - preparation and less the emergence of pupa rate (2.1) and by (62%) at a concentration of 5 g / L of bio - preparation comparison with control (3.2) and by (96%) for bacteria isolated from water. and on kill adult, The highest killing rate for males (1.6) and by (32%) and females (1.5) and (30%) at a concentration of 5 g / l of bio - preparation and less killing of the male rate (0.5) and (10%) and females (0.4) and by (13%), comparison with control males (0.1) and by (2%) and females (0.1) and by (2%) for the isolated bacteria from soil. The highest kill the male rate (0.8) and by (32%) and females (0.7) and (30%) at a concentration of 5 g / L of bio - preparation, and less killing of the male rate (0.2) and (4%) and females (0.4) and by (13%) at a concentration of 1 g / L of bio - preparation comparison with control males (0.1) and by (2%) and females (0.1) and by (2%)for the isolated bacteria from water and one female productivity of egg reached its highest production rate eggs 84.5 eggs per female at a concentration of 1 g / L of bio - preparation, and less egg production rate of 54.5 eggs per female at a concentration of 5 g / L of bio - preparation comparison with control eggs productivity rate of 87.5 egg female for isolated bacteria from soil reaching its highest egg production rate (85%) eggs per female at a concentration of 1 g / L of bio - preparation, and less egg production rate (79.5%) eggs per female at a concentration of 5 g / L of bio - preparation, compared with treatment control productivity rate of whites (85.5%) eggs per female for isolated bacteria from the water

تقييم فعالية مستخلصات مستنبت القمح Triticum aestivum في بعض الانواع البكتيرية المسببة لاخماج مختلفة == Evaluation The Activity of Germinated Wheat Extractions Triticum Aestivum on Some Bacterial Species That Cause Different Infections

اسم المؤلف: اسماء عيسى محمود
اسم المشرف: رشيد حميد حسن
الموضوع العام: علوم الحياة
السنة: 2012
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: The study had included isolation and diagnosis of three hundred samples for both sexes their ages were between (1 - 60) years of the inpatients and outpatients in Tikrit Teaching Hospital and General Samarraa Hospital during the period from 1/9 / 2011 to 1/3/2012. The infections were distributed among the burns infections, the wounds, urinary tract infections (UTI) and female genital tract infections (High vagina). This study clarified that (192) sample from the total samples showed bacterial growth in percentage 64%. The results of this study indicated to the proportion 58% with (UTI) by (87) bacterial isolation were produced from (150) isolation. The percentage of burns infections were 81.81% from (55) sample which distributed among the first, second and third class burns. The burns of second and third class gave bacterial growth in a proportion 100% while the first class burns gave 64.28%. Concerning the wounds infections, bacterial growth was 62.9% from (62) sample. The infections proportion of (H.V) was 63.63% from (33) sample. The results of the current study showed that the infections in females were higher that in males with respect to (UTI), while there was not considered difference between the males and females about the burns and wounds infections. Differently, the (H.V) infections were just for the females in the ages between (18 - 45). It was clear according to the results of diagnosis that Escherichia coli had the highest proportion of isolations 25.52% from the total isolates followed by Staphylococcus aureus 18.23% Pseudomonas aeruginosa 15.63%, both Klebsiella pneumoniae and Proteus miribilis 10.42% Staphylococcus epidermidis 4.69% Citrobacter freundii 4.17%, Staphylococcus saprophyticus 3.64% both Serratia marcescens and Enterococcus faecalis 2.6%, Streptococcus pyogenes 1.56% and Staphylococcus intermedius had the least percentage 0.5% by one from (192) isolate. The sensitivity of these isolates was tested by (10) antibiotic which included quinolones, aminoglycosides, beta - lactams and tetracyclines antibiotics. Among these antibiotics imipenem and ciprofloxacin were more effective, while the other antibiotics were different either active or not active at all.The wheat germinating was used in this study with the ages (36 - 48) hour and its activity was tested by using some bacterial kinds. After completing several quantitative and qualitative tests on this germinating, the carbohydrates proportion was between 67 - 70%, the protein proportion by folin method was between 10 - 8%, the lipids proportion was 1.5%.Some mineral elements in germinated wheat were estimated which include Calcium, Iron, Magnesium, Potassium, Copper, Manganese, Zinc, Selenium, and Sodium element, active materials were also estimated in the wheat germinating extracts which had inhibitory effect on the microbiology. It was clear by the tests that the germinating contains a lot of Phenolic compounds, Terpeniods, glycosides and steroids. These materials were extracted by using some extracts included the cold water extracts and hot water extracts, alcoholic extracts (ethanol and methanol) and phenolic extract. All the extracts prepared in concentrations (25, 50, 100, 200) mg/ ml. This study showed that the phenolic extract had high effect on the bacterial kinds in comparison with the methanolic and ethanolic extracts at (200) mg/ ml concentration while the cold water extract had less effect on these bacterial isolates in comparison with the above extracts, finally the hot water extract had no inhibitory effect against any isolated bacterial type with all the concentrations. The inhibitory action of these extracts was compared with some antibiotics as a control sample. These antibiotics represents different groups of antibiotics and which contained Imipenem, Ciprofloxacin, Gentamicin, Ceftazidime in addition to Augmentin.In this study the minimum inhibitory concentration (MIC) of the fenolic, alcoholic and watery extracts was determined and the concentration was different according to the extract type, the bacterial type and the isolation site.

دراسة تاثير مستخلصات نباتي القرنفل والخروع على نمو بعض الفطريات المنتجة لانزيم البروتييز والمسببة لالتهاب الاذن الخارجية == Study Effect of Syzigium Aromaticum And Ricinus Communis Extracts on Growth of Some Fungi That Produce Protease And Cause Otitis Externa

اسم المؤلف: بتول عمران ذيب
اسم المشرف: خليل ابراهيم بندر النعيمي | ذكرى احمد حمادة
الموضوع العام: علوم الحياة
السنة: 2009
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الفطريات
الصفحات الاولى:
المستخلص: ضمنت الدراسة عزل وتشخيص الفطريات من الاذن الخارجية في مستشفى تكريت التعليمي - وحدة الاذن والانف والحنجرة والعيادات الخاصة في مدينة تكريت, خلال الاشهر تموز, اب وايلول 2008. جمعت 100 مسحة اذنية من المرضى المشخصة اصابتهم بالتهاب الاذن الخارجية الفطري من قبل | The study was included isolation and identification of fungi from the external ear in Tikrit teaching hospital, the unit of Ear, Nose, and throat (E.N.T.) and Privete clinics in the city of Tikrit during the months July, august, and September2008. 100 ear swabs were collected from the patiants whose diagnosed by Otologists with Otomycosis, and 50 ear swaps were collected from healthy persons in the same city and period time, These samples were examined by dierect microscopic examination treated with KOH 10% with hot flame to ensure the fungal infections, and Culturing on Sabouraud, s Dextrose Agar was done for isolation and identification of types of fungi which responseple for infection, The result of direct microscopic examination and culture showed that 76(76%) were positive for fungi, 64 were positive by direct microscopic examination and culture, and 12 posative in culture only. The most common fungi isolated from patient were Aspergillus Spp.36). (%22 followed by Candida Spp. (%67. 10), Trichophyton Spp(%6.5) , and Penicillium spp. (%3.94%), Aspergills niger appeared as the predominant fungi (%84. 36) followed by Aspergillus flavus (%36. 22), Candida albicans (42. 18%), Aspergills terrous (7.89%) , Candida kruzi, Penicillium spp (3.94%), Trichophyton mentagrophyte, Trichophyton verrucosum (63. 2%) and Trichophyton scheonlieni (%1.31), and no fungi were isolate from the healthy person. Higher percentage of fungi infection were recorded in female 46(60.52%) than male 30(39.47%). and the higher percentage for fungal infection was recorded in the age group (31 - 40) with percentage (23.68%). fungal ear infection was recorded with highest percentage in right ear 47 (61.84%), and for the left ear 29 (38.15%). and high rate of infection was recorded in august (73.69%), Higher percentage of infection was recorded in patient house wives (52.36%), the itching was the most commen symptom , The most habits used to clean the ear by dearty body (51.31%), Ahigher percentage (76.4%) recorded in non educated persons. The results of active ingredients test for Syzigium aromaticum and Ricinus communis revealed that clove dry flower buds contain Flavonoides, Glycosides, Tannins, Volatile oils, Terpenes, Resins, and Phenols, while castor leaves contain Flavonoids, Tannins, Volatile oils, Terpenes, Resins, Saponins and Phenols, pH of clove bud 4.3 and pH of castor leaves 5.8. Aqueous and alcoholic extracts were Prepeared, which belongs to Syzigium aromaticum and Ricinus communis under the study, commercial oil for clove and seed castor were used in the study, the effects of inhibition of Aqueous and Alcoholic extracts and commercial oil had been studied on the growth and protease production on the three fungi which were showed the high ratio appeared among the isolation sample under the study with 5 concentration 10, 20, 30, 40, 50 (mg /ml), and the affected rate of these extracts and oils under the study on fungi were varied. The relation between Aqueous extract of clove bud and fungi growing was increasing relation, While Alcoholic Extract and commercial oil showed preference in the inhibition of the isolated fungi grown while its effect on Candida albicans was increase with increase the concentration, The effect of aqueous and Alcoholic Extract of castor leaves on Moulds showed reverse effect. on the other hand the commercial oil effect was slight effect, and both extracts did not showed effect on the Candida albicans as well as the Commercial oil.When adding Clove Extracts and Commercial oil and in all concentrations dose not gave any production of the Protease enzyme in the solid media, whereas the extracts of castor leaves and commercial oil were showed effects on the production of Protease enzyme, and Aspergills niger was more effected and these extracts did not showed an effect on the Candida albicans.Aqueous and Alcoholic extract and commercial oil to the Clove revealed higher influence rate than the effect of the Castor leaves extract , and in a different rate

التحري عن بعـض عوامل الفوعة لبكتريا المكورات Enterococci المعوية المقاومة للمضاد الحيوي الفانكومايسين == Detection of Some Virulence Factors of Vancomycin - Resistant Enterococci

اسم المؤلف: حيدر صباح كاظم الخماسي
اسم المشرف: مي طالب فليح
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم الحصول على (20) عزله لبكتريا المكورات المعوية البرازية من اصل (135) عينة جمعت من مستشفى مدينة الطب ومستشفى الكندي وهي كما ياتي (15 عينة الادرار, 60 عينة دم, 50 عينة من قنوات الجذر للاسنان و10 عينة من الحروق).اختبرت الحساسية لهذه العزلات العشرون لـ 11 | Clinical sampling was carried out between September and December 2013, Twenty Enterococcus faecalis isolates were obtained from 135 clinical specimens. The samples included of patients in Medical City Hospital and Al - kindy Hospital (15 urine, 60 blood, 50 root canal and 10 wound swabs) Antibiotics susceptibility test for 20 isolates was done against 11 antibiotics, it was revealed that the isolates showed multi drug resistance were (18) isolates. The vancomycin susceptibility was determined by the minimum inhibitory concentration (MIC). Resistant and intermediate resistant to vancomycin was distributed among isolates at a ratio of 65% and 20%, respectively. Imipenem was found to be the most bactericidal agent against E. faecalis isolates E. faecalis virulence factors were detected phenotypically, The results showed that all isolates (100%) were hemolysin, protease and aggregation substance producer. 30% of isolates showed an ability to produce gelatinase. While (40.7%) of the isolates were a lipase producer. The results of the tube method showed that all E. faecalis isolates (100%) were slime layer and biofilm producer but the amount of adherent layer were different among the isolates ranged from strong to moderate and weak.The extracted DNA was subjected to Polymerase chain reaction (PCR) technique in a monoplex pattern to amplify the virulence factor Enterococcal Surface Protein (esp) which is chromosomal, results of this investigation showed that 20 (100%) E. faecalis isolates gave the amplicon size 933 base pair for the esp gene.The genetic determinants of Vancomycin - Resistant vanA and vanB genes were amplified using monoplex and multiplex PCR techniques in order to identify vancomycin resistant (van+) and sensitive (lacking van) among (13) E. faecalis. The vanA, vanB genes were detected in 11 and 4 E. faecalis isolates, respectively. The results of monoplex and multiplex PCR revealed that the molecular weight of vanA and vanB genes were 550 and nearly 600 bp, respectively. The results revealed that the vanA and vanB amplicons have a genetic variation in their molecular weight during the electrophoresis of PCR product.

تشخيص المسببات البكتيرية والفايروسية لخمج الاغشية السحائية في مدينة الموصل == Detection of Bacterial And Viral Causes of Meningitis in Mosul

اسم المؤلف: قاسم مصطفى خالد
اسم المشرف: علي صالح حسين الجبوري | اميرة محمود محمد الراوي
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: تضمنت الدراسة اجراء عدد من الاختبارات العيانية والكيميائية على عينات سائل النخاع الشوكي (CSF) cerebrospinal fluid البالغة 152 عينة والتي جمعت من الاطفال الراقدين في مستشفى الخنساء التعليمي ومستشفى ابن الاثير التعليمي في مدينة الموصل لحديثي الولادة لغاية 1 | This study includes performing of macroscopical and chemical and Microbial tests on 152 samples of cerebrospinal fluid (CSF) collected from hospitalized children in Al - khansaa and Ibn - Al - Atheer teaching hospitals in Mosul city with ages ranged from newbornn to 12 years old. Suspected to be infected with meningitis during the interval from August 2009 till April 2010.The results showed that 46.1% of samples were normal with respect to colour and appearance as well as variations in the levels of the three variables including glucose, protein and total cell count with differential cells in the studied samples.The study also included isolation and identification of gram positive and negative bacteria, morphological, cultural and biochemical tests and API were done depending on identification of the isolated bacteria.Many types of isolated gram positive bacteria belonged to the two genera staphylococcus and streptococcus; the results revealed that S.aureus was the more predominant within gram positive causes. Well as many types of gram negative bacteria were isolated represented with Klebsiella pneumoniae, Haemophilus influenzae, Acinetobacter spp. E.coli and Neisseria meningitidis with different percentages with dominance of K.pneumonia and H.influenzae.The study also included screening of some viral causes of meningitis from (35) CSF samples which gave negative culture results by using qualitative Enzyme immunoassays technique using Minividas apparatus.The results showed positive results with 14.2% for two types of virus including Herpes simplex virus (HSV) and Cytomegalo virus (CMV).The study indicated that males were more compared with Females with 55.9% and 44.1% to males and females respectively and highest infection rate occurred in 1 day - 2 years old.Statistical analysis showed the presence of significant correlation between sex, age and the three varieties glucose, protein and total cell count the results showed there is no significant differences between the studied factors.The study also includes sensitivity for some antibiotics, the results showed that gram positive bacteria were the more sensitive to Vancomycin and Cefatriaxone as well as Clindamycin while gram negative bacteria showed sensitivity and resistance percentages of bacteria in general were varied to other antibiotics under study.

دراسة الانماط المصلية وعوامل الضراوة لبكتريا E.coli المعزولة من حالات خمج المجاري البولية لدى النساء في محافظة ديالى == A Study On Serotypes And Virulence Factors of Escherichia Coli Isolated From Women With Urinary Tract Infections In Diyala Province

اسم المؤلف: لارة محمود شفيق السوره ميري
اسم المشرف: محمد خليفة خضير
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: شملت هذه الدراسة 350 عينة ادرار وسطي ومنها تم الحصول على 100عزلة من بكتريا الاشريشيا القولونية المعزولة من النساء المصابات بالتهابات المجاري البولية في مستشفى خانقين العام, ومستشفى بعقوبةالتعليمي, ومستشفى البتول للولادة والاطفال في محافظة ديالى من الفترة | The study was included 350 samples of Mid - stream urine and which has been obtained 100 isolates of Escherichia coli from women with Urinary Tract Infections in Khanaqin General Hospital and Baaquba Teaching Hospital and Al - Batool Hospital in Diyala Province. All samples were collected from 2013/10/15 to 18/2/2014. All bacterial isolates were identified by the biochemical cultural, serological teast and microbial characteristics and confirmed by VITEKA2, API - 20E system. The serological test was performed by slide agglutination test for the urine isolates and 19 of them 19% gave positive results for the polyvalent antisera O26, O55, O111, O119, O126. The results showed the susceptibility 57 bacterial isolates to produce hemolysin with percentage 57%, and production of bacteriocin Form71%. The production of biofilm by local isolates were detected in three ways, isolates of Escherichia coli has shown its ability to produce biofilm by a manner ELISA, adhesion Surface methods, and a Congo - red methods as apercentage 90%, 83%, 78% respectively. The results showed that 88% from Escherichia coli isolates were able to produce ? - lactamase enzymes by rapid iodometric method, and 4% of isolates have the ability to produce of the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, while 2% of isolates have the ability to produce of Metalo ? - lactamase enzymes by using the Imp - EDTA combination dis casmanaged. The sensitivity of these isolates were tested against (16) antibiotics, the results induct that E.coli had resistance to the antibiotics : Augmentin, Aztreonam, Ampicillin, Co - trimoxazole, Chloramphenicol with the rates 100%, 93%, 92%, 89% and 86% respectively. The isolates were more sensitive to Ceftazidime, Gentamycin, Ciprofloxacin, and Cefixime with resistance rate 41%, 38%, 35% and 30% respectively. The antibiotics Imipenem and Tobramycin were more sensitive with sensitive rate 100% and 80% respectively. Multiple resistance pattern for antibiotic divided into two groups, first included 69 isolates 69% which were resistant to 6 - 10 antibiotics, while second included 31 isolates 31% were resistant to11 - 15antibiotics.

بعض المؤشرات المناعية عند مرضى الحروق في مدينة بعقوبة == Some Immunological Parameters In Burn Patients In Baquba City

اسم المؤلف: عبد الله خزعل محسن القيسي
اسم المشرف: عباس عبود فرحان الدليمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: اجريت الدراسة في مختبرات مستشفى بعقوبة التعليمي التابعة لمحافظة ديالى خلال المدة الزمنية المحصورة من 1 /2014/10 الى 2015/ 7 /1 وتضمنت هذه الدراسة ثلاث مجاميع هي مجموعة المرضى تالفت من 70 شخصا من المرضى الراقدين في ردهة الحروق التابعة للمستشفى اعلاه بواقع | The study was conducted at Baquba teaching hospital in Diyala province during the period October 2014 - July 2015. The study included three groups, a total of 70 patients who were admitted at burn department they were 33 (47.14%) female and 37 (52.85%) male with age range from (1 - 60) year, 40 (57.14%) of 1 - 20 year, 25 (35.71%) of 21 - 40 year and 5 (7.14%) of 41 - 60 year, and three degrees of burns, 15 (21.42%) of the first degree of burn, 34 (48.57%) of the second degree of burn and 21 (30%) of the third degree of burn. The polices of the two people who did not suffer from any medical condition, the first group consisted of 30 people to 14 (46.66%) female and 16 (53.33%) male. And the second set especially cellular Dynamics consist of 10 people included 5 (50%) females and 5 (50%) male, note that sets the polices were distributed to the same age groups above. C - Reactive protein is positive measured effectively using latex examination and rely on the presence of altlasn from non existence, while conducted examinations alghlobulinat immuneglobulins IgG and IgM, and complement components C3 and C4 by single Radial Immuno diffusion and conducted tests kinematics cellular IL - 2 and IL - 6 using adsorption - linked immunosorbent assay technique. The results of the study showed significant difference when level (P < 0.05) for CRP value reaching up to his cause the immune index in patients group compared with the control group. While results of IgG concentration showed great differences at the level of (P < 0.01) in female patients with average (1103.02 mg/dl) and males average (1136.05 mg/dl) compared to the control group females average (977.90 mg/dl) and males average (1038.00 mg/dl). While results of IgM concentration showed great differences (P<0.01) in female patients with average range (242.84 mg/dl) and males average (233.50 mg/dl) compared to the control group females average (124.37 mg/dl) and males an average of (93.10 mg/dl). While results of C3 concertration showed significant differences (P<0.05) in female patients with average range (94.87 mg/dl) and males an average of (131.40 mg/dl), compared to the control group females average (98.6 mg/dl) and males average (93.10 mg/dl). While the results of C4 concentration showed significant differences (P<0.05) in female patients with average range (41.31 mg/dl) and male average (37.78 mg/dl) compared to the control group females average (24.62 mg/dl) and males an average of (25.50 mg/dl). The results of IL - 2 concentration showed great differences (P<0.05) in male patients only average (30.16 pg/ml) compared to the control group average of (29.66 pg/ml). While results of IL - 6 concentration showed significant differences (P<0.01) in female patients with average range (63.39 pg/ml) average male (66.47 pg/ml) compared to the control group females average (2.48 pg/ml) and males average (22.80 pg/ml). Moreover the results of immunological indices the significant differences between age groups and burning scores of people with burns in comparison with the control group

انتشار انزيمات بيتالاكتاميز نوع OXA بين عزلات بكتيريا الزوائف الزنجارية في مدينة الديوانية == Prevalence of OXA Beta - Lactamaes of Pseudomonas Aeruginosa In Al - Diwaniya City

اسم المؤلف: رنا مشعل سالم
اسم المشرف: سيوف خومان علوان الرماحي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: The aim of This study is to determine the prevalence of OXA ? - lactamase in pseudomonas aeruginosa isolates from clinical and other environmental cases in Al - Diwaniya Teaching Hospital using phenotypic and molecular methods.The Samples of the study were collected from various sources and they were 390 samples for the period from November 2011 to March 2012, and they includ (292 clinical specimens and 98 environmental sample), the results of cultural and biochemical tests showed that 50 isolates (39 of them were from clinical cases and 11 were from environmental samples), belonged to ps. aeruginosa, and their diagnosis were confirmed by 16s - ribosomal RNA, and the study showed that all the isolates containing the 16s - ribosomal RNA gene, which represents the designed diagnostic gene in this study.The results of this study showed that the higher rate of P. aeruginosa isolates was due to burns infections (23.68 %) , followed by respiratory tract infections (sputum) that were (15.38 %) and then the case to otitis media infections (12.5 %), and lastly that due to urinary tract infections (10.49 %), while the rate isolation from the environmental samples was (5.11 %) from the floor of burns department, followed by (10.8 %) from the medical instruments of department workers.It was noted from this study that the age and gender of the patients have some effect on the infection by P. aeruginosa it was recorded that the higher rate of infection was in patients older then 61 year old, and regarding gender, it was found that females are more infected than male, especially in urinary tract infections. The study showed that there is high rate of infection in the admitted patients, in older people and in those who need urinary catheterization, in addition to those with severe burns and those in the intensive care units. Drug sensitivity test had been carried out for all the bacterial isolates to 22 types of antibiotics by disk diffusion method of Kirby - Bauer, and this study had indicated that there was relatively high resistance from P. aeruginosa to ? - lactam antibiotics, aminoglycoseides, and fluoroquinolone. The results also showed that the studied isolates has possibility of producing broad - spectrum ? - lactames enzymes, and this is supported by the resistance of this bacteria to the third generation cephalosporins and to Alaztronam, it was also shown that there was difference in the resistance to carbapenems antibiotics, the resistance rate to imipenem and meropenem were 16% and 64 %, respectively. Polymyxin B and Polymyxin E also called (Colistin) antibiotics were also used, and the resistance rate %0 for Colistin and %4 for Polymyxin B, that means there was two isolates which are resistant to Polymyxin B and this resistance was a challenge to the success of therapeutic efforts. this study noted that there was 22 (44%) isolates which were resistant to at least three types of antibiotics, and regarded as multi - drug resistant (MDR) and Extensive - drug resistance (XDR) isolates were26 (52%) which was the higher rate among the three types of resistance, The third type of resistance was 2 (4%) which represents the resistance to all types of studied antibiotics (PDR). The ability of these isolates to produce broad - spectrum OXA ? - lactames enzymes groups was investigated thrrough detection of presence of genes blaOXA - 10, blaLCR - 1, blaOXA - 18, blaOXA - 1, blaOXA - 2 in these isolates by using the polymerization chain reaction enzyme technology] (PCR) it showed 50/50 (100%) isolates contain blaOXA - 10 gene, which belongs to the OXA group I, and the results of this study showed no amplification results for blaOXA - 18, blaLCR - 1, blaOXA - 1, blaOXA - 2 genes, which belong to the main groups of broadspectrum OXA ? - lactames enzymes which are OXAgroup II, OXAgroup III and OXA groupV respectively except OXA18 enzyme

دراسة تاثير مستخلصات القرفة (Cinnamomum zeylanicum) والسماق (Rhus coriaria) في التثبيط المايكروبي وبعض المعايير الحيوية في ذكور الجرذان == Study The Effects of Cinnamon (Cinnamomum Zeylanicum) And Sumac (Rhus Coriaria) Extracts on Microbial Inhibition And Some Biological Parameters in Rats Male

اسم المؤلف: فراس عدنان حسين الزيدي
اسم المشرف: كركز محمد ثلج الجبوري
الموضوع العام: علوم الحياة
السنة: 2010
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: صممت الدراسة في مختبرات قسم علوم الحياة / كلية التربية وقسم علوم الاغذية / كلية الزراعة في جامعة تكريت, اذ تضمنت الدراسة التعرف على المواد الفعالة في المستخلصات المائية والكحولية في كل من قلف القرفة وبذور السماق واختبار فعاليتها التثبيطية عند تركيز 10, 25 | This study was conducted in Biology department laboratory / Education college and Food science department in Agriculture college in Tikrit University. the study was conducted to investigated to the aquaies and alcoholic activities extracts from each Cinnamon bark and sumac seeds and assay of inhibition activity concentration at 10, 25, 50 and 100 mg/wells against gram positive and negative bacteria and phagocytosis Immunity level after injection by E.coli type, further more to study the effect it in some physiological parameters in male rats after oral administration for 8 days by 50 and 100 mg/kg from rats body weight from each aquais and alcoholic extracts. The results was illustrated the aquaies and alcoholic extracts from each Cinnamon bark and sumac seeds were contains the active groups from compound such as Tannins, Resins, Phenols, Flavonoids, Alkaloids, Glycosides, Coumarins. while the effects was found the aquaies extracts a high significantly effects (p < 0.05) from alcoholic by inhibition ability when assay. The Minimum inhibition concentration (MIC) from each ones which mass 50 and 125 mg/ml from culture media to inhibition concentration by E.coli and S.pyogen cinnamon bark aquaies extract. it was 75 and 100 mg/ml from culture media to inhibition concentration by E.coli and S.pyogen from alcohol extracting its inhibiting efficiency throw the measure of the diameter of the inhibiting area that has shown that the gratest effect was E.coli bacteria which its diameter of inhibiting area 17 mm and when the concentration is 100 mg/wells from the alcoholic and aquaies extracting and the less effect is S.pyogen and S.aeruginosa in case of the extraction from cinnamon bark it was 17 mm the diameter of inhibiting area of E.coli bacteria. when the concentration 100 mg/wells from aquaies extracting from sumac. whether with alcohol extraction, the bacterial type were similar in their sensitivity in diameter of inhibiting from 18 to 20 mm but the oral administration of the extract on the level of phagocytosis process through the injection of rats in affixed number from E.coli bacteria. The result was illustrated the type of concentration from each cinnamon bark and sumac seeds such as aquaies and alcoholic was caused an increase in the phagocytosis process through the significantly decrease in the number of bacteria with increase of the concentration from the groups that oral administration from extraction that there number wear (zero) on the concentration 100 mg/kg from rats body weight when the time is 120 minute which was equal with effincy of the ciprofloxacin antibiotic on the concentration 5 mg/kg from rats body weight. The result also showed the effect of oral administration from the extraction of cinnamon bark and sumac seeds such as aquaies and alcoholic, that significantly caused the decreased in the concentration of the hemoglobin and Total count red blood cells and the packed cell volume on the concentration 100 mg/kg from the weight but the measures have not significantly changed the parameters MCV, MCH, MCHC with increase of concentration from extraction compared with the group of controlling. The white blood cells also decreased in the significantly aquaeis and alcoholic extracting from the cinnamon bark it was 6.8 × 103/mm3 on the oral administration of the rats from the concentration 100 mg/kg from the body weight of the aquaeis extracting, and 6.8 × 103/mm3 on the oral administration of the rats from the concentration 100 mg/kg from the body weight of the alcoholic extracting, That numbers have not affected during the oral administration from the extractions of sumac only on the concentration 100 mg/kg from the body weight of the alcohol extracting which was 5.6 × 103/mm3 the increase was in the ratio of the number of the neutrophils in the whole extraction alcoholic and aquaies for the both of plants. While the effect of oral administration from the extraction in the lipids profiles was clear in the decrease significantly in the concentration of the glucose and albumin and Urea and uric acid and creatinine and triglyceride and, cholesterol, and the low density lipoproteins (LDL) and very low density lipoproteins (VLDL), significant increasing in globulin and high density lipoproteins (HDL) in aquaies and alcoholic extraction of sumac seeds while the value of Total protein and HDL is not significantly differtited in aquaies and alcoholic extraction moreover oral administration of the both of plants. The enzyme activity value was included significantly decreased of the Alkaline phosphtase activity value with the increased orally administration from aquaies and alcoholic extracts from both of plants, while increased significantly Aspartames amenotransferase (AST) activity value in all cinnamon extracts while was significantly decreased when administrate from sumac seeds extract, But don’t significantly differ Alanine amenotransferase (ALT) activity value in all concentration from aquaies and alcoholic extracts from both of plants.

علاقة مستوى الزنك والنحاس ببعض المعايير المناعية في الاصابة بداء المقوسات في النساء في محافظة كركوك مع محاولة علاجية في الفئران المختبرية == Relation of Zinc And Copper Levels With Some Immunological Markers in Women With Toxoplamosis in Kirkuk Governorate With Trial To Treatment in Mice

اسم المؤلف: عبير عباس علي
اسم المشرف: حسين ساهر اسود العبيدي | شهاب احمد محمد
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: تم دراسة 100امراة مصابة بداء المقوسات Toxoplasmosis الذي يسببه الطفيلي المقوسة الكوندية Toxoplasma gondii للفترة من اذار 2007 ولغاية نيسان 2008 راجعن العيادة الاستشارية التابعة لمستشفى كركوك العام في مدينة كركوك. اظهرت نتائج الدراسة الحالية ان 56 % من الن | A total of 100 patients suffering from Toxoplasmosis which is infected by the parasite Toxoplasma gondii have been dealt with as subject of the sample during the period from March 2007 to April 2008 at the Consultant Clinic of Kirkuk General Hospital. The results of the present study showed that 56% of the subjects were pregnant and 44%were not. The highest percentage of the infected subjects was of 26 - 30 years old as 30.37% among the pregnant women and 36.35% among the non - pregnant women of the age mentioned above. The highest percentage 37.5% of abortion of the first period of pregnancy was in pregnant women, and 45.53% in non - pregnant women. The results of the study also showed more cases of infected women among those who bred animals in their living places as 69%. It is also found that the highest percentage of the infected women 64% were of those who use the running water for drinking. Moreover, it is noticed that there is an increase in the percentage of the infected women among the uneducated women 38%. The study showed that the percentage of the infection is proportional to residence 63%, the infection increase among women living in rural areas. The percentage of T - lymphocytes in peripheral blood of patients were also measured through measuring the percentage of the CD4+ and CD8+cells by using fluorescent microscope.The results showed a significant decrease (p<0.05) in this type of cells.The rate of percentage of CD4+ of the patient's were 31.704±0.116 while the rate of CD4+ in the peripheral blood of the control group was 62.260±0.237.The rate of percentages of CD8+ 19.382±0.080, whereas it was 31.680±0.307 of the control group.The rates of the percentages of CD4+ to CD8+ were also measured, the results showed a significant decrease (p<0.05) in the rate of the percentage in the patient's group 1.642 while it was 1.968 in the control group. The statistic analysis showed significant differences between these tow groups (p<0.05). The intensity of Zinc and Copper has been measured in the serum of the infected women via use of Atomic absorption instrument, a significant decrease (p <0.05) appeared in the rate of Zinc intensity in the serum on the infected women as compared with the control group.The rate of the intensity in patient's group was 4.65% ± 2.628µmol /l while in the control group it was 15.353± 1.640 µmol /l.A significant increase (p<0.05)was also noticed in the intensity of Copper in the patient's group (28.589±4.335 µmol /l)whereas it was 12.854±1.501 µmol /l in the control group. The index of the specific antibodies IgM and IgG related to Toxoplasma gondii was measured by ELISA method, a significant increase (p<0.05) was observed in the value of IgM and IgG in the patient's group as compared with the control group. The rate of index for IgM in the patient's group was 1.354±0.05 while it was 0.163±0.05 in the control group. The rate of value of IgG in the patient's group was 1.163±0.074 whereas it was 0.358±0.033 in the control group. In the present study, Toxoplasma gondii was isolated and diagnosed from human as first attempt, and then the infection was created in laboratory mice as an essential step for growing the parasite. The second step was studying the effect of some selected drugs on the experimental treatment for disease of toxoplasmosis created in the mice. The drugs are : Pyrimethamin, Clarithromycin, Spiramycin, Ivermectin.The results of the study showed the effects of these drugs on the tissue cyst formed in the brain and lungs. The number of tissue cysts in the brain of the mouse treated with pyrimethamin was 14.8 ±1.067 after 28 days. Significant differences appeared when this result compared with the control group in which it was 27.4±1.029. The number of tissue cysts formed in lung, tissue in the mouse treated with the same drug after the same period of the time was 6.2±0.583.This showed a significant differences as compared with the control group in which it was 10.2±0.583. No significant differences appeared between both groups as for the treatment by Clarithromycin antibiotic as far as the number of the tissue cysts formed in the brain after 28 days is concerned. The number was 23.8 ±0.86 as compared with the control group 38.6±1.029.The same compared is true of the lungs, tissue. The number of the tissue cysts was 23.8±0.86 as compared with the control group 28.4±1.805. Concerning the antibiotic Spiramycin, the results showed the effectiveness of it in decreasing the tissue cysts formed in the brain.The significant differences were (p<0.05), the rate of the cysts was 9.6±0.6 as compared with the control group in which it was 22.8±1.067.The same effectiveness appeared on the lungs, tissue as the rate of tissue cysts after 28 days of treatment was 4.6±0.509 as compared with the control group in which was 17±1.14 and the significant differences were (p=0.000).The Ivermectin drug showed a great effectiveness in minimizing the number of the tissue cysts formed in the brain and the lungs. The numbers of the tissue cysts formed after 28 days were 3.2±0.374 and 1±0.316 respectively; significant differences are observed when these numbers are compared with the numbers of the control group which were 22±1.843 and 19.4±1.435. Thus, the use of Ivermectin drug is considered as a pioneer and fruitful study since this drug has not been used for the treatment of Toxoplasmosis.

استخلاص وتوصيف المنشط السطحي الحيوي Rhamnolipid من بكتيريا Pseudomonas aeruginosa المعزولة من بعض الحالات السريرية والبيئية == Extraction And Identification of Rhamnolipid Biosurfactant From Pesudomonas Aeruginosa Isolates From Clinical And Environmental Cases A Thesis Submitted

اسم المؤلف: مها هاني توفيق الخزرجي
اسم المشرف: ندى صباح رزوقي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: هدف البحث الى التعرف على فاعلية التدريس باستراتيجية التعلم بالتعاقد في تحصيل مادة علم الاحياء لدى طلاب الصف الثاني المتوسط وتفكيرهم الابداعي. وللتحقق من ذلك تم صياغة الفرضيتين الصفريتين الاتيتين : 1 - لا يوجد فرق ذو دلالة احصائية عند مستوى (05.0) بين مت | 50 isolates of the bacteria Pseudomonas aeruginosa was obtained from 201 clinical samples were distributed between Burn Specialist Hospital and teaching laboratories from patients with burns, infected wounds, middle ear infection, urinary tract infection and respiratory tract infection for a period from 1st February till 1st May. And we obtained 50 bacterial isolates from 20 samples from contaminated and non - contaminated soils were distributed different areas of Baghdad for a period of 1st May 2013 till 1st August.Number of cultural microscopically, biochemical and sensitivity to antibiotics tests had been, than diagnosis was confirmed by API20E system.These isolates was tested for ability to production of biosurfactants (rhamnolipid) by haemolysis, oil spreasding test, calculate value of emulisification factor (E24) and measuring surface tension for liquid media. Tow isolates (PS42 and PP8) had been selected, first one was from soil samples and other was from pathological samples because of they have highest productivity, haemolysis ability, oil spreading, highest emulisification factor value and highest in lowering surface tension, there for these tow isolates selected for study their inhibitory activity against types of bacteria. The rhamnolipid was extracted from tow isolates P. aeruginosa PP8 and P. aeruginosa PS42 by using mixture of solvents as was obtained 15.45 g and 18.25 g per liter of each of the PP8 and PS42 respectively. The rhamnolipid was diagnosed by thin layer chromatography technology (TLC) and high - performance liquid chromatography (HPLC) showed that tow bacteria produced three types of rhamnolipids (mono - , di - rhamnolipid and rhamnolipid A). The rhamonlipid efficiency was tested for inhibitory activity against bacteria by measuring diameter of inhibitory zone surrounding holes and discs. The inhibitory activity was high against Bacillus cersus bacteria followed by P. aeruginosa, than Staphylococcus aeraus and the lowest one was E. colli. The inhibitory activity for biosurfactant was approached to inhibitory activity for industrial surfactants. Also in this study has been determined minimum inhibitory concentration (MIC) value and Minimum Bactericidal Concentration (MBC) value for biosurfactants and the results varied depending on different types of biosurfactants and type of bacteria and the lowest values for MIC and MBC of biosurfactant produced by P. aeruginosa PS42 on growth of B. cereus and reached to 16.It was first time at local level for testing inhibitory activity of biosurfactant against pathogenic bacteria S. aureus and P. aeruginosa which was studied in vivo (injuried skin) after introduction these pathogens experimentally into laboratory mice with clinical symptoms appeared in injured skin after 48 hours and then treated mice groups with of 30 mg \ ml concentration of biosurfactants produced from isolates of bacterial (P. aeruginosa PP8 and P. aeruginosa PS42), resulted in a decrease in the time required for healing as found from the results obtained by the different length of period needed for healing (depending on the nature of injury and type of microorganism that causes injury).The mice had been recovered after 5 days when used biosurfactant at concentration 30 mg/ml produced P. aeruginosa PS42 followed by mice recovered after 6 days when used biosurfactants 30 mg/ml produced by P. aeruginosa PP8 in comparison with control group which recovered after 12 days for mice infected with S. aureus. In mice infected with P. aeruginosa recovered after 10 days when used biosurfactant produced by P. aeruginosa PS42 at concentration 30mg/ml and followed by mice recovered after 12 days when used biosurfactant produced by P. aeruginosa PP8 at concentration 30mg/ml in comparison with control group which recovered after 17 days.

دراسة بعض مظاهر امراضية الخمج بالمتحولة النسيجية بين الاشخاص في مدينة سامراء == A Stud Y of Some Aspects of Entamoeba Histolytica Pathogenicity Among Peoples in Samarra

اسم المؤلف: صلاح عبد حسن السامرائي
اسم المشرف: ابراهيم شعبان داوود | حسين ساهر اسود
الموضوع العام: علوم الحياة
السنة: 2008
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الكلمات الدلالية:
  • الاحياء المجهرية - الطفيليات
الصفحات الاولى:
المستخلص: شملت هذه الدراسة فحص (540) عينة براز من المصابين بالاسهال والمراجعين لمستشفى سامراء العام وذلك خلال الفترة الممتدة بين 1/10/2007الى 1/6/2008 فحصت العينات بطريقة المسحة المباشرة المزدوجة للكشف عن الاطوار الخضرية والمتكيسة لطفيلي اميبا الزحار. واظهرت النتائ | The current study dealt with examination of (540) stool samples for patients with diarrhea who visited the General Hospital of Samarra during 1/10/2007 - 1/6/2008. The samples were examined by double wet direct smear method to identify the trophozoite and cyst stages of Entamoeba histolytica. The results showed that (112) were infected with amoebic dysentery. The total rate was (20.74 %). (262) are males with a rate of infection (19.84 %), whereas, the number of females was (278) with an infection rate (21.58 %). The study showed an increase rate of infection among the rural population, it was (25.71 %), whereas the urban population rate was (16.6 %). The study covered all ages beginning from less than a year to 75 years old. A highest rate of infection was recorded by (10 - 19) years old, it was (29.4%). The study also, found out that a highest rate of infection was during school months, particularly during November (27.5 %). Examinations of complete blood picture which included Hemoglobin concentration (Hb), packed cells volume (PCV), white blood cells count (WBCc), and erythrocyte sedimentation rate (ESR) were done on all patients infected with amoebic dysentery. The results were compared with those of healthy ones (Control Group). The results showed significant differences between the two groups. According to the significant one (P < 0.05), it recorded a decrease (16.9%) in the (PCV) and (25.1%) in the (Hb) and an increase (241.8%) in the (ESR) and (51.5%) in the (WBCc) for those ones infected with amoebic dysentery if compared with the control group. Clinical biochemistry examinations were performed, on blood sugar (Bs), electrolytes such as Sodium (Na+), Potassium (K+), Calcium (Ca++), Magnesium (Mg++), liver functions tests : total serum bilirubine(TSB), tranceaminase enzyme(GOT, GPT), alkaline phosphetase (AlP), total protein (TP) and albumin (Alb). The results showed that there are significant differences between persons infected with amoebic dysentery and the healthy ones according to the significant level (P < 0.05) in these examinations. The study showed a decrease(21.6%) in the (Bs) mean concentration, (16.8%) in the(K+) mean concentration, (5.8%) in the(Ca++) mean concentration, (4.5%)in the (Mg++) mean concentration, (10.5 %) in the (Na+) mean concentration , (17.2%) in the (TP) mean concentration and(12.2%) in the (Alb) mean concentration and increase (83.7%) in the (Alp) mean concentration, (52.1%) in the(TSB) mean concentration, (81.1%)in the (GPT) mean concentration, (59.9%)in the (GOT) mean concentration and(22.8%) in the (globulin) mean concentration in the persons infected with amoebic dysentery compared with the mean concentrations in healthy ones control group.

التوصيف الجزيئي لبعض مسببات الاخماج الجلدية في محافظة الديوانية وتاثير جزيئات الفضة النانوية في نموها == Molecular Characterization of Some Dermatophytic Fungi In Al - Diwania Province And The Effect of Silver Nano Particles On Their Growth

اسم المؤلف: نور جاسم محمد
اسم المشرف: نيران عبيد جاسم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة للتحري عن الفطريات المسببة للامراض الجلدية في الانسان حيث تم جمع 100عينة من الاشخاص المشكوك باصابتهم بالفطريات الجلدية من المرضى المراجعين لمستشفى الديوانية التعليمي من بداية تشرين الاول 2013 وحتى نهاية مايس 2014 وكانت هذه النماذج ماخوذة | This study was conducted to investigate the fungi that causes skin diseases of human. One hundred samples were collected from people who suspected to infect with fungal skin disease from patients coming to Al - Diwaniya teaching hospital from beginning of October 2013 till the end of May 2014. These samples from (skin, hair and nail). All samples that taken from patient were subjected to direct examinations by using wet touch of (KOH 10%) solution. At the same time the samples were grew on sabroud media to determine the type of fungal skin diseases of human. The direct examination shows positive results of 72 samples which represent 72% while for laboratory growth results, its shows 65 samples with positive results 65%. There are three types of Trichophyton fungus had been isolated which are T.rubrum 28 isolates, T.mentagrophytes 16 isolates and T.schoenleinii 4 isolates and there was one type of genus Microsporum which is M.canis 24 isolates. The results show that Tinea corporis infection is the common and its more among females than males where it is 26.39% and 13.89% respectively, then Tinea capitis which is 19.4% in males which is higher than in females which represents 13.89% while Tinea unguium was in females more than males which was in females 16.67% while in males 9.72%. The percentage of fungal skin infection was differ according to the location where the number of infections in the rural area for all types of Tinea were more than the number of infection in the urban area where the number of infected people with Tinea corporis was 18 which represent 25% while in the urban the number was 11 which represent 18.28% while the number of infected people with Tinea capitis in the rural area were 16 which represent 22.22% and the infected in the urban were 8 which represent 11.11%. While Tinea unguium the number of infected people in the rural 10 which represent 13.89% and in the urban 9 people which represent 12.5%. The diagnosis of isolated fungi depended on phenotype of fungal growth and microscopic examination, in addition to special tests for diagnosing skin fungal infections. Also the PCR was used for diagnosis and the work had been completed by using genotypic tree for fine diagnosis and detection the fungal species. The result were send to the genes bank site to be sure about the fungus type through comparison with the registered fungi in this site and by using MEGA6 program for genetic tree analysis of type (unweighted pair group method with arithmetic mean). The results of analysis shows clear converge of local Trichophyton spp. Isolates with Trichophyton rubrum in comparison with other types that appears in the genetic tree analysis while Microsporium diagnosed through genetic tree analysis of type (up GMA tree) and the result of analysis shows clear converge of local Microsporium spp. Isolates with Microsporium canis compared with other types of genetic tree analysis. The effect of silver nanoparticles that is used against growth of T.rubrum, T.mentagrophytes and M.canis. The results show that all the concentrations used were effective in stopping of growth of T.mentagrophytes when deal with the standard measure when dealt with concentration of 4 mg/l the more effective in stopping of radiating growth of the fungus. Regarding the fungus T.rubrum the concentrations of 4 mg/l more effective in stopping of growth while there was no difference for 1mg/liter concentration. Regarding the fungus M.canis, the mentioned concentrations had large effect on fungal colonization growth compared with the standard and this fungus more than other fungi affected by nano silver ions
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