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دراسة المقاومة المتعددة للمضادات الحيوية لعصيات التدرن لدى مرضى التدرن الرئوي == A Study On Drug Resistance Of Mycobacterium Tuberculosis Isolated From Pulmonary Tuberculosis Patients
Author name:
انمار ليث طالب الحسني
Supervisor name:
عامر رشيد النجار | احمد اسمر منخي
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Master
University:
Mustansiriyah University
Language:
English
University location:
Baghdad
First pages:
19T1132 - p.pdf
Abstract:
تعتبر عزلات جراثيم التدرن المقاومه للادوية من المشاكل الرئيسية في معالجة مرضى التدرن حول العالم. , ولاهميته اجريت هذه الدراسة لتسليط الضوء اكثر حول الحساسيه الدوائية بواسطة الطرق المختبرية التقليدية وعلى الوسط الصلب ومقارنته مع الطرق التشخيصية المختبرية ا | Drug resistant tuberculosis remained as major problems in the treatment of tuberculosis patients in the world. A prospective study was conducted to evaluate drug susceptibility testing (DST) by classical methods on solid media as compared with advance and molecular Laboratory diagnostic methods (Bactec MGIT 960 and GeneXpert MTB - RIF ). The study included the estimation of the first line anti - TB drugs, including (Rifampicin,Isonizid,Streptomycin and Etambutol) by solid media (Lowenstein - Jensen medium) and Bactec MGIT 960 system. In addition, the use of rapid molecular diagnostic method by GeneXpert MTB/RIF assay. Seventy five TB patients attending the National Reference Laboratory of Tuberculosis /medical city - Baghdad from December 2015 to June 2016 were included in this study. Patients were classified into two main groups : selected MDR patients, which were referred from different hospitals and health respiratory center in Iraq, represented 46 in order to compare different laboratory methods to detect of DST isolates, while 29 were randomly isolated and identified , the study matched by age and gender were also included. This study revealed that the drug resistance tuberculosis to Rifampicin was 47 (62.7%), Isonizid 41 (54.7%), Streptomycin 42 (56.0%) and Ethambutol 38 (50.7%). Respectively , on the golden stander in which solid media were used.The molecular and advance technique (GeneXpert and Bactec MGIT 960) as applied to estimation of drug resistance tuberculosis, the results of drug susceptibility testing (DST), as showed highly sensitivity and Accuracy, when compared with golden stander( LJ media) especially in detected of primary drugs (Rifampicin and Isoniazid) and showed lower sensitivity and accuracy to detected of the secondary drugs (Streptomycin and Ethambutol). The Multiple drug resistance (MDR - TB) case from selective samples was found to be almost similar to that found in other countries of the middle east and other regions in the world. While in the randomly identified samples, the rate of MDR - TB was lower than the referred (previously treated) cases.The GeneXpert MTB - RIF system is rapid molecular methods that used in many countries to identify of MTB and Rifampicin detected during 2 hours only, that used in this study. The high Rifampicin resistance and other drugs of local isolates could be due to abuse of the drug,defaulter and relapse of retreating cases (previously treated).
