دراسة التعبير المناعي النسيجي الكيميائي لبعض المعلمات المناعية في مختلف درجات سرطان البروستات == Study The Immunohistochemical Expression Of Some Immunological Markers In Different Grades Of Prostate Cancer
Author name:
حسن هادي زريج
Supervisor name:
زينب فاضل عاشور | وسن عبد الاله باقر
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Master
University:
Mustansiriyah University
Language:
English
University location:
Baghdad
First pages:
19T1135 - p.pdf
Abstract:
Prostate cancer is the most common malignancy and the second leading cause of cancer related death among males worldwide. Prostate cancer developed in prostate , a gland that is located below the bladder and just in front of rectum.Inflammation is a fundamental physiological process that can arise in any tissue in response to traumatic, infectiousor autoimmune injury. Transforming growth factor beta one (TGF - ?I) is a potential regulator of prostate cancer cell growth that signals through a heteromeric complex composed of type I and type II receptors.TGF - ?R II is an important receptor , because TGF - ? bind to receptor II to initiate it's signaling, then receptor II recruits receptor I to initiate signal transduction. Interleukin - 17 (IL - 17) is a pro - inflammatory cytokine produced by T - helper 17 (Th17) cells contributes toboth the processes by playing a dual role in the antitumor immunity. On one hand, IL - 17 encourages an antitumor cytotoxic T cell response leading to tumor regression. On the other hand, by promoting angiogenesis and egress of tumor cells from the primary focus, so IL - 17 promotes tumor growth.This study was carried out to establish the correlation between expression of Transforming growth factor beta one(TGF - ?I), Transforming growth factor beta receptor one (TGF - ?RI) and Transforming growth factor beta receptor two (TGF - ?RII) and prostate cancer progression. Also to establish the role of IL - 17 in prostate cancer development.The study consisted of 16 patients with poorly differentiated malignancy (PDM) ,whose age ranged (48 - 89) years, with mean age of (71.2) years , and 25 patients with moderately differentiated malignancy(MDM) , whose age ranged (31 - 83) years , with mean age of (66.6) years , and 46 patients with benign prostatic hyperplasia (BPH) , whose age ranged (27 - 85) years , with mean age of (64.3) years. Allpatients were diagnosed surgically by consultant physicians in Baghdad hospital for specialist surgeries , Al - Yarmook teaching hospital ,as well as AL - Hilla teaching hospital , and 20 normal tissue sample taken from cadaver, whose age ranged (17 - 28) years , with mean age of (21.8) years , where chosen as a control group. The work was conducted in Iraqi center for cancer and medical genetic researches. The study were performed during the period from March 2014 to December 2014).Immunohistochemistry (IHC) technique was used to detect the level of expression of TGF - ?I , TGF - ?RI , TGF - ?RII and IL - 17 protein in tissues of patients and healthy control groups.The current study revealed that there was significant difference in mean levels of TGF - ?I protein expression between each of poorly and moderately differentiated malignancy compared with healthy control subjects with P - value of 0.001 , but there was no significant difference in mean level of TGF - ?Iprotein expression between benign prostatic hyperplasia and healthy control subjects with P - value of 0.398. Also there was no significant difference between poorly and moderately differentiated malignancy in mean level of TGF - ?I protein expression.TGF - ?R1 protein was expressed in 3 (18.7%) and 14 (56%) of poorly and moderately differentiated malignancy respectively , with weak immunostaining reaction was the most frequent score. There was significant difference in mean level of TGF - ?RI protein expression among all studied groups. TGF - ?RII protein was expressed in 6 (37.5%) and 22 (88%) of poorly and moderately differentiated malignancy respectively ,with weak immunostaining reaction was the most frequent score. There was significant difference in mean level of TGF - ?RII protein expression among all studied groups.IL - 17 immunohistochemical expression was detected in tissues of all studied groups , the results showed that moderate immunostaining reaction was the most frequent score among them. There was significant difference in mean levels of IL - 17 protein expression among all studied groups.We concluded that there was statistically significant association between the loss ofexpression of TGF - ?1 signaling receptors, especially TGF - ?RI, andincreasing grades of malignancy in prostate cancer. These resultssuggest a potential mechanism for prostate cancer cells toescape the growth inhibitory effect of TGF - ? and thus, leadingto a more malignant phenotype.
