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التشخيص الجزيئي لتجرثم الدم لدى الاطفال حديثي الولادة بواسطة استخدام تفاعلات البلمرة المتسلسل في مدينة الحلة == Molecular Detection of Neonatal Sepsis By Using PCR In Hilla City
Author name:
نور حامد عباس المرزوك
Supervisor name:
الهام عباس بنيان الساعدي | ميساء صالح الشكري
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Master
University:
University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language:
English
University location:
Babylon
First pages:
19T1123 - p.pdf
Abstract:
في هذه الدراسة, تم جمع 85 عينة دم من 85 مريض مصابين بتسمم الدم (حسب التشخيص السريري الاولي للطبيب), تتراوح اعمارهم بين 1 - 28 يوم من كلا الجنسين (الذكور 53 والاناث 32) للفترة من تشرين الثاني 2014 الى اذار 2015.توضح نتائج هذه الدراسة ان (52,9%) طفلا حديث | Neonatal sepsis can be defined as any systemic bacterial infection confirmed by a positive blood culture in the first month of life. In this study, (85) blood specimens were collected from (85) patients with suspected septicemia, age ranged between 1to 28 days from both sexes (53 males and 32 females) during the period from November 2014 to March 2015.The results of this study revealed that (52.9%) are presented with late onset sepsis (infected during 7 - 28 days of their life), while the rest (47.1%) of the neonates are presented with early onset sepsis (infected during the first seven days of their life). The collected samples were investigated for bacterial isolation. Bacterial culture was positive in (7.06%) patients versus (92.9%) patients revealed a negative bacterial culture. The most common types of bacteria isolated were three isolates of Klebsiella spp. (50.0%), followed by one isolate Staphylococcus aureus (16.67%), one isolate E. coli (16.67%), and one isolate Enterobacter spp. (16.67%).The virulence factors of some bacterial isolates in this study were studied. The capsule was present in all bacteria isolated in this study (100%) except for Enterobacter spp. which did not possess the capsule.Colonization factor antigens were detected in all isolates. It was found that only Klebsiella spp. and E. coli (100%) have the ability to produce CFAI and CFAIII. Whereas Enterobacter spp. and Staphylococcus aureus (100%) have the ability to produce colonization factor antigen I only. The results also showed that all isolates of Klebsiella spp. and Enterobacter spp. did not produce hemolysin, while all isolates of Staphylococcus aureus and E. coli were able to produce hemolysin.Extracellular protease was also investigated. It was found that (100%) of all bacteria isolated in this study were able to produce extracellular protease.Biofilm formation was investigated in all bacterial species, and the results showed that all isolates of Gram negative bacteria and Gram positive bacteria form a biofilm.The ability of some bacteria to produce bacteriocin was tested and the results showed that E. coli and Klebsiella spp. were able to produce bacteriocin.In - vitro, the susceptibility of bacteria towards some antibiotics included in this study revealed that the amikacin and kanamycin are the most effective antibiotics to different types of pathogenic bacteria. Finally, molecular detection for bacterial sepsis was done by using specific primers (16 sRNA, rpoB and its). It was found that 16 sRNA genes were present in (20%) of samples and rpoB gene present in (18.8%). While its gene used for detection of fungi revealed negative results in all samples.
