الصورة البكتيرية والنسيجية المرافقة لالتهاب اللوزتين المزمن والادينويد عند الاطفال == Bacteriological And Histopathological Profile Associated With Chronic Tonsillitis And Adenoid Hypertrophy In Children
Author name:
رشا محسن كاظم الحسيني
Supervisor name:
حبيب صاحب نهر | صفاء حسين الطريحي
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Master
University:
University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language:
English
University location:
Babylon
First pages:
19T1128 - p.pdf
Abstract:
This study aimed to investigate the bacterial profile of surface and core of infected tonsils and adenoid tissues and histopathological examination of these organs.A total of 122 samples represented by 63 excised tonsils and 59 excised adenoids in addition to 57 blood samples were collected from 70 patients who were referred to Hilla Teaching Hospital (Ear, Nose and Throat unit) in Hilla city within a period of four months from November 2015 to February 2016. Those patients weresuffering from chronic tonsillitis and adenoid hypertrophy. The ages of those patients ranged from 2 to 15 years, 41 (59%) of them were males and 29 (41%) were females.The study included three main parts. The first one was the bacterial diagnosis based on relied diagnostic procedures. Second part was the detection of antistreptolysin O (ASO) by using latex agglutination test, while the third part included histopathological study to detect the pathological lesions that occur during these infections.The results indicated that, the most age group being susceptible for chronic tonsillitis and adenoid hypertrophy was the group of 7 - 10 years, represented 33 patients with a percentage of (47%). Among this group, males were more susceptible with percentage of 20 (29%) compared to female patients13 (19%).The surface of all samples (tonsil and adenoid) revealed positive results for bacterial culture, while in the core of tonsil only 44 samples (70%) were positive for bacterial culture while 19 samples (30%) were negative results (no growth). As in tonsils, the core of adenoid revealed positive results in 32 (54%) samples, while 27 (46%) samples were negative results.The suspected isolates were fully identified by using bacteriological, biochemical and Vitek 2 techniques (for two isolates because of those isolates give variable biochemical test results which confused their diagnosis that lead to using of vitek system technique to diagnosis them, these isolates were rare bacteria that infect tonsil and adenoid). The most common types of bacterial isolates were Staphylococcus aureus which accounted for 75 isolates (23.6%), followed by Streptococcus pyogenes 61isolates (19%), Escherichia coli 57 isolates (18%), Klebsiellae pneumoniae 55 isolates (17.3%), Hemophilus influenza 37 isolates (11.6%), Sphingomonas paucimobilis 14 isolates (4.4%), Staphylococcus epidermides 7 isolates (2.2%), Pseudomonas aeruginosae 6 isolates (2%), Pseudomonas stutzeri 5 isolates (1.6%), and Staphylococcus saprophyticus only one isolate (0.3%).Regarding the bacterial population, there is a difference between tonsils / adenoid surface and tonsil/ adenoid core culture were the most predominant isolate from tonsil surface was Staphylococcus aureus and K. pneumoniae from tonsillar core, while from adenoid surface was Streptococcus pyogenes whereas E. coli from adenoid core.The ASO titer in patients with or without group A streptococcal infections were determined by using latex agglutination test. The results revealed that 8 samples (14%) exhibited positive results, while 49 samples (86%) exhibited negative results out of 57 samples. The results showed statistically no significant differences between patient age groups but showed highly significant differences between males and females.Eight specimens were taking for histopathological examination randomly, four samples were adenoids and four samples were tonsils. The histopathology examination of tonsil and adenoid showed the presence of lymphoid hyperplasia,multiple lymphoid follicles, vascular congestion, fibrosis, germinal centers, infiltration of inflammatory cells, necrosis and apoptosis of tissues
