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التشخيص المقارن المصلي والجزئي لداء المقوسات وبعض الاصابات الفيروسية لبعض النساء الحوامل اللوات يعانينن من الاجهاض في محافظة النجف الاشرف == Serological And Molecular Comparative Diagnosis of Toxoplasmosis And Certain Types of Viral Infections (Torch) In Aborted Women In Al - Najaf Province
Author name:
ذكرى عبد الله محمود المياحي
Supervisor name:
باقر عبيس سلطان | صباح نعمة محمد الفتلاوي
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Doctorate
University:
University of Kufa - Faculty Of Medicine - Department Of Microbiology
Language:
English
University location:
Najaf
First pages:
19T1107 - p.pdf
Abstract:
اجريت هذه الدراسة في محافظه النجف الاشرف / العراق، وتضمنت جمع عينات من دم النساء الحوامل اللواتي يعانين من الاجهاض اول مرة او متكررة وكان عدد الحالات 57 حالة. اعمارهن يتراوح بين 15سنة الى40 سنة واللواتي يراجعن مستشفى الزهراء التعليمي والمختبر المركزي وبع | Toxoplasmosis and viral infections such as Rubella , Cytomegalovirus and Herpes virus (TORCH) are feared risk during pregnancy. The rate of toxoplasmosis and of subclinical infected infants at birth with the risk of late manifestation is still unclear, whereas such data are fairly well - known for Rubella , Cytomegalovirus and Herpes virus. The respective major diagnostic issues in pregnancy, the laboratory diagnosis, and its rational use in combination with clinical information are presented , also the value of passive prophylaxis, therapy, and prenatal diagnosis as well as the possible management for diminishing the infection problems in pregnancy.Aims of study : The study was aimed to evaluate the ELISA result for Toxoplasma, rubella, cytomegalovirus (CMV) and Herpes virus (TORCH) IgM and IgG and compare the results of the ELISA with RT - PCR to arbitrate discordant results.Methodology : The present study was carried out on 57 pregnant women, all of them had with bad obstetric history (BOH) of abortion twice or more. Patients were between 15 - 40 years of age, and they were attending Al - Zahraa Maternity and pediatric Teaching Hospital, Al - Hakeem Hospital and some private clinics, in AL - Najaf province during the period from May 2012 till May 2013. Serological evaluation for TORCH infections was carried out by using IgM and IgG Enzyme Linked Immunosorbent Assay (ELISA ) method and molecular technique ( DNA; RNA - RT - PCR). Each blood sample was divided into two part , one part plane tube for separation of serum that were screened for the presence IgM and IgG antibodies against Toxoplasma gondii ,Cytomegalovirus Rubella virus and Herpes simplex virus by using (ELISA test ).The tests were performed according to manufactures instruction.The other part (blood )EDTA tube was used for DNA 0f detection Toxoplasma , CMV and Herpes simplex virus , while RNA extraction of Rubella virus.The blood and serum sample were stored in refrigerator (2 - 8C?) before the analysis.The extracted DNA and RNA were stored at - 20C? till used within 24 hours. All ELISA Results samples were compared with Real - Time PCR result. Statistical analysis : Statistical program SPSS version (20.0) was used to study the significant difference between tests in each type of antibody (IgM and IgG). (X2 - test) was used after detection of normal distribution to the data and appropriate P<0.001 consider significant. Results : Both IgM / IgG was Seropositive to T. Gondii , Rubella , CMV and HSV - 2 which were 49.12/80.70%, 21.05/49.12% , 45.61/70.18% and 1.21/36.20% respectively. Samples were seropositive to IgM and results were retested by RT - PCR method. Maximum number of IgM /IgG seropositive cases of aborted women was 19(32.75%)/57 that associated with Toxoplasma ,Rubella and CMV infection by RT - PCR ( 10(17.54%), 6( 10.53%) and 3(5.26%) respectively ). The ELISA TORCH IgM and IgG assays provided results comparable to RT - PCR indicated significant differences among Toxoplasma and viral infection. The ELISA TORCH infection IgM and IgG assays showed a lower specificity with for Toxoplasma IgM (p=0.001), poor for CMV IgM and moderate for Rubella virus IgM.Conclusion : The accurate diagnosis of TORCH infection should be done by molecular method.
