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تعقب الليشمانيا الجلدية بالاختبارات التحليلية الوبائية والطفيلية والجزيئية والكيمياحياتية == Tracking of Cutaneous Leishmaniasis By Epidemiological, Parasitological, Molecular And Biochemical Analysis
Author name:
سندس نصیف الحجیمي
Supervisor name:
باقر عبیس سلطان | محسن عبد الحسین الظالمي
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Doctorate
University:
University of Kufa - Faculty Of Medicine - Department Of Microbiology
Language:
English
University location:
Najaf
First pages:
19T1103 - p.pdf
Abstract:
The present study was performed to identify the species and strain of Leishmania parasite isolated from different endemic areas. It was carried out on cutaneous leishmaniasis (CL) in five Iraqi provinces as following : Al - Najaf province ( Al - Hakeem and Al - Sadder teaching hospitals), Babylon province ( Marjan hospital ), Al - Qadisya province (AL - Sadder Teaching hospital ), Karbala province (Al - Hussain General Hospital) and Kut province (Al - Zehraa Teaching and Al - Kerama Hospitals). It covered seven hospitals as a field of investigations from October 2010 to December 2012.Cellulose acetate electrophoresis has been performed in Walter Reed Institute of researches in USA. A total of 126 cases comprising 48(38%) females and 78(62%) males were studied. The highest infected age group was 21 - 30 years with a rate of 27.8% and the lowest rate was 11.9% at the age group of 10 and less years.Geographical distribution of CL among hospital patients indicated that rural areas were with highest rate (54%) while in urban areas were with(46%).The number of ulcers per one patient differs for each patient. The highest rate of multiple lesions was 73% in comparison with single lesion (27%).The type of infection showed that disease was in wet type (82.5%) more than in dry type (17.5%). Direct smears were made by lesion aspiration,skin scraping and discharged blood methods , by which 90 (71%) patients gave positive result under light microscope.Modified NNN medium and RPMI - 1640 with fetal bovine serum followed by sub - culture in drosophila Schniders media were performed for each case. Out of 126 cases,83(65%) gave positive growth. Further characterization of the causative parasite species and strain made by molecular and biochemical techniques.Out of 83 postive growth culture,only 52 specimens were studied by nested - PCR, using kinetoplast minicircle fraction amplification to detecte the species of parasite.It was found that 45(86.5%) cases in the generation of a 560 bp DNA and 7(13.4%)patients displayed a fragment of 750 bp, corresponding to L. major and L. tropica, respectively.This test revealed that L. tropica and L. major are the causative agents of infection with dominant shifting to the L. major.Cellulose acetate electrophoresis (CAE) was performed for 20 mass cultivated cultures. Isoenzyme profiles of these isolates were compared with reference strains of Leishmania spp. using cellulose acetate electrophoresis and 4 enzyme systems (glucose phosphateisomerase,leucil phosphate,manose phosphate isomerase and 6phosphogluconate dehydrogenase). The results showed that L.major(LV39) isolate were in 20 mass cultivated culture.Most of the CL patients in Najaf province(2011,2012) were from Haidariya in north district. High prevalence of disease was observed in Autumn and Winter.The highest number of cases was recorded during February and December.
