Author name: نجم الازيرجاوي

Supervisor name: هيفاء عبد علي الشمري

General topic: Biology

Specific topic: Life Science

Degree: Master

University: University of Thi-Qar - College Of Science

Language: English

University location: Dhi Qar

First pages:

Abstract: This study is adescriptive exploratory study in Thi - Qar providence for some breast cancer patients . This study aims to assess the relationship between gene expression for each type four of the collagen (IVCollagen) and gene expression positive for both estrogen receptor ER - Eestrogen - receptor and progesterone PR - Progestron receptor and fairs tumor HER2 (Human epidermal growth factor receptor) By using chemistry of dyes immunological technique and the nuclear regulatory regions Argyrophilic nucleolar organiser region (AgNOR(. The present study addresses the identification of the type IV collagen content in the basement membrane of epithelial tissue of the breast cancer patients in Thi qar and its relationship to nuclear organization region and maintain its relationship with hormonal receptors and progesterone genetic factor and the extent of their association with the occurrence of breast cancer.Fifty tissue samples are collected from Hussein Teaching Hospital inThi Qar and some private laboratories in the providence each case where data included that the most important factor is age, where he found that the highest percentage of injuries, age (55 - 46 years) increased by 38%. And mixed samples, either partial removal of the tumor or the eradication of whole breast. After collecting samples saved each formalin concentration of 10% for 24 hours at a minimum and then buried with parafin wax and completed the work by histological methods own work.The use of special select number of collagen type IV and determine the type of hormonal receptor positive or negative and also used several positive pathological tissues of the tumor to determine the human epidermal growth factor receptor by using silver nitrate to study the nuclear organization regions. Results of the current study showed that there is a positive relationship between collagen gene expression of type IV and between fairs oncogene(HER2 human epidermal growth factor receptor), as observed the presence of collagen type IV in tissue sections of tumors with HER2 negative gene expression of genetic factor, and with the positive expression for each Estrogen receptor positive ( estrogen positive ) and progesterone receptor positive( Progstrogen positive).Results of this study also showesthe presence of collagen or lack in histological sections of the tumors with positive gene expression of the HER2 oncogene fairs as well as the non - existence of collagen in the sections taken from tumors with negative expression of estrogen and progesterone receptors. The results of the current study also showed that there is a clear positive relationship between nuclear organization regions and between Argyrophilic nucleolar organiser region (AgNOR) Fairs oncogene HER2, as it was noted the presence of many of the nuclear regions of the dye - derived tissue sections of tumors with positive gene expression of the HER2 oncogene fairs, while nuclear chromosomal regions did not appear in the sections derived from tumors with negative expression of estrogen receptor and progesterone hormone. The results of the current study also showed that there is a lack of positive gene expression of collagen type IV, the presence of a number of chromosome regions nucleuse (Argyrophilic nucleolar organiser region (AgNOR)).