اسم المؤلف: حازم علي حسين

اسم المشرف: مفيد جليل عوض | مؤيد عمران الغزالي

الموضوع العام: الطب

السنة: 2017

الموضوع الدقيق: الكيمياء الحياتية السريرية

الدرجة: ماجستير

الجامعة: جامعة بابل - كلية الطب - قسم الكيمياء الحياتية

اللغة: الانكليزية

مكان الجامعة: بابل

الصفحات الاولى:

المستخلص: لمنع تراكم الحديد في الجسم، وخاصة لمرضى داء ترسب الاصبغة الدموية،او الذين يحتاجون الى نقل دم بصورة مستمرة، لابد من عمل مركب معه يعمل على استخراجه.نبتة العليق الاحمر تحتوي على كمية عالية من حامض التانيك وهو مركب متعدد الفينولات للنبات يعمل على مسك او حجز | To prevent iron overload, especially for those patient with hemochromatosis or those having chronic blood transfusion, iron must be chelated and excreted, Rubus Idaeus containing high quantity of tannic acid which is a plant polyphenol, chelate iron and excreted from the body. The present study aimed to evaluate the activity and efficiency of tannic acid in Rubus Idaeus fruit to reduce or eliminate iron overload through enhance direct impact on some of the body,s vital biological activities by using fresh crude plant as herbal medicine. This study is Cohort study designed. It is monitoring, detection, and investigation study that included some of groups that completely under control(control), and other group which induced iron overload , the iron overload group is subdivided to 5 groups, one of this sub gorups is treated with desferal(Deferroxamine),and the rest 3groups giving 3 different doses of pure fruit of Rubus Idaeus, while the least group is not treated with fruit or with desferalThe study included (60) Albino male mice divided into six groups with count (10) mice for each. First group was labeled as control (A). The other five groups received intra peritoneal injections of iron - dextran at a dose of 100 mg/kg/day B.w. (one dose every two days for 3 weeks) are the iron overload groups ,which are classified as a following : B1 group ,in which the iron overload induced mice treated with 100 mg/kg/day of Rubus Idaeus fruit, B2 group, in which the iron overload induced mice treated with 200 mg/kg/day of Rubus Idaeus fruit,B3 group ,in which the iron overload induced mice treated with 300 mg/kg/day of Rubus Idaeus fruit, group(C),in which the iron overload induced mice received 493mg/kg/day desferal by subcutaneous injection for 21 days beginning on the day following the first iron dextran injection, group (D) ,in which the iron overload induced mice neither received desferal nor Rubus Idaeus fruit. The experimental work was carried out in the animal house and in the Clinical Biochemistry Department / College of Medicine / Babylon University during the period from (October 2015 to August 2016). High - Performance Liquid Chromatography (HPLC) technique has been used for measurement of tannic acid in fruit in mice's serum. Enzyme Linked Immunosorbent Assay (ELISA) has been used for the measurement of serum ferritin, UV - Vis Spectrophotometer has been used for determination of GSH - Px and GST concentrations, while Graphite Furnace Atomic Absorption Spectrophotometer technique (GFAAS) has been used for concentration measurement of trace elements. There were hematological study have been carried out using special required equipment, protein analysis by electrophoresis has been used for mice's serum to detect protein changes for all groups. Results showed that Rubus Idaeus fruit contain good acceptable concentration of tannic acid up to (853 µg/gm),amount of trace element in the fruit plant was 14.12 ppm for Zinc,33.62 ppm for Iron,3.5ppm for copper(Cu),311.06 ppb for selenium(Se),40.13 ppb for chromium(Cr). Results also showed highly significant increase of Serum ferritin, hemoglobin levels, GST activity, Iron and Copper concentration, and decreased in chromium in iron overload group (D) (P<0.001) when compared with control group (A), while there were highly significant decrease of these parameters and increased chromium in group (B2) and group (C) as compared with control groups. Results also showed highly significant decrease of GSH - Px activity, Zinc and Selenium concentration, and increased in hemoglobin levels in group (D) (P<0.001) when compared with the control group, while there were highly significant increase of these parameters and decreased in hemoglobin level in group (B2) and (C)as compared with control and (A) groups. The study have been found positive effect of Rubus Idaeus on abnormal parameters and support the biological system which was clear in group (B2) and (C) which demonstrated that there were significant inhibitions to decrease or increase of these parameters than those in normal. The present study found that fruit of Rubus Idaeus in dose(200 mg/kg/day)in mouse(16.2mg/kg/day in human) gives more reasonable results than other 2 doses (100mg/kg,300mg/kg) in treatment of iron overload ,100mg/kg had no effect in reducing iron overload,300mg/kg chelate iron so more that causing anemia, while this study found a negative correlation between Ferritin and GSH - Px activity, serum chromium and Selenium concentration. Results also showed that there is a changes in protein bands in samples, new bands occurs in samples of induced iron overload group (D) due to increased production of iron regulating proteins. While these bands are absent in groups (A),(B2)