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تاثير الانزيمين الكلوكواميليز والكلوكوز اوكسيديز في تثبيط بكتريا Streptococcus mutans المسببة لتسوس الاسنان == The Effect of Glucoamylase And Glucose Oxidase On Inhibition of Streptococcus Mutans Causes The Dental Caries

اسم المؤلف: بيداء عبود حسن الجنابي
اسم المشرف: زهرة محسن علي | محمد عبد الله جبر
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: تضمنت الدراسة استخلاص الكلوكواميليز والكلوكوز اوكسيديز من الفطرين Aspergillus niger وPenicillum notatum على التوالي وتنقية وتوصيف الانزيمين لغرض زيادة تركيز بيروكسيد الهيدروجين المحفز لانزيم اللاكتوبيروكسيد الموجود باللعاب على تكوين ايونات الهايبوثايوسيان | This study aims at producing glucoamylase and glucose oxidase from the Aspergillus niger and Penicillium notatum respectively , purifying and characterizing the enzymes to increase H2O2 concentration which induce lactoperoxidase in saliva to the formation of hypothiocyanite ion to inhibite Streptococcus mutans which causes the dental caries. For glucoamylase and glucose oxidase production, the results shown the the highest enzymes production were occurred using the synthetic medium, it gave high titer of glucoamylase and glucose oxidase activity (0.212, 0.605 U/ml) respectively, the optimum incubation period for glucoamylase was occurred after 96 h (0.125 U/ml), while the maximum activity of glucose oxidase was occurred after 72 h (0.662 U/ml), the optimum temperature for enzymes production was occurred in 30°C, (0.257, 0.690 U/ml) respectively, the maximum production of glucoamylase and glucose oxidase were occurred in the pH = 6 (0.174, 0.391 U/ml) respectively, the optimum nitrogen source for enzymes production was yeast extract and potassium nitrate (0.157, 0.571 U/ml) respectively, the optimum carbon source for glucoamylase and glucose oxidase production was starch and glucose (0.167, 0.459 U/ml) respectively. For glucoamylase and glucose oxidase purification, the results found the ammonium sulfate (80 %) was selected as the best ratio for precipitate of glucoamylase , the specific activity reached to (3.626 U/mg) , with a purification fold (1.241) and yield (0.38) % , while the (60 %) was selected as the best ratio for precipitate of glucose oxidase, the specific activity reached to (10.304 U/mg) , with a purification fold (1.219) and yield (0.34) % , two peaks of glucoamylase were appeared in the gel filtration the specific activity of the first form (4.195 U/mg) with purification fold (1.436), while the second (31.214 U/mg) with purification fold (10.689), while one peak of glucose oxidase was appeared in the gel filtration, the specific activity was reached (62.382 U/mg) with purification fold (7.385). For characterization of enzymes, the results reported the highest activity of glucoamylase (Aand B) occurred in pH = 8, 6.5 (0.253U/ml, 0.511 U/ml) respectively , while the maximum activity of glucose oxidase (1.289 U/ml) in pH = 5.5, the optimum temperature of glucoamylase (Aand B) activity (0.243U/ml, 0.703 U/ml) in 40, 30 °C respectively , while the optimum temperature of glucose oxidase activity (1.424 U/ml) in 45 °C, the optimum pH for glucoamylase (B) stability ranging between (5.5 - 6.5), while the optimum pH for glucose oxidase stability ranging between (5 - 6) , the optimum temperature for glucoamylase (B) stability ranging between (10 - 35) °C for 30 min, while the optimum temperature for glucose oxidase stability ranging between (25 - 35) °C for 15min, the molecular weights of glucoamylase (Aand B) approximately 52 & 66 kDa respectively using the electrophoresis technique, while approximately 68 kDa for glucoamylase (B) using the gel filtration technique, the molecular weight of glucose oxidase approximately 78 and 87 kDa electrophoresis electrophoresis and gel filtration respectively, the values of Km and Vmax of glucoamylase and glucose oxidase were (2.4 mM , 9.6 mM/min and 19.6 mM, 7.5 mM/min) respectively. The result of this study showed the Streptococcus mutans growth was killed in the using the first and the second concentration of glucoamylase and glucose oxidase (0.09, 0.009 and 0.3, 0.03 µl) respectively , while the growth was appered in third concentration of enzymes (0.0009, 0.003 µl) respectively. The NaF, ZnF2 , C 12H 7 Cl 3 FNaO2 , NaCl , NaHCO3 and KSCN with (0.5 and 1M ) leads to increase the enzymes activity , while Na3PO4 , SrCl2 , Fe2O3 and Ca(HCO3) 2 caused decreased the activity of enzymes when treated with (0.5 and1 M) from these solutions, the glucoamylase activity also decreased when it treated with (0.5 and 1M) from KNO3 solution but this solution increased the activity of glucose oxidase when it treated with (0.5 and 1 M) from this solution

الاصابة ببكتريا Helicobacter pylori وعلاقتها بمرض تصلب الشرايين القلبية == Correlation Between Helicobacter Pylori Infection And Atherosclerotic Heart Disease

اسم المؤلف: رواء سعدي سلمان
اسم المشرف: مي خليل اسماعيل
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: مرض قصور الشرايين التاجية هو النتيجة النهائية لتراكم اللويحات العصيدية ضمن جدران الشرايين التاجية مسببة نقص الاوكسجين وبالتالي حدوث مرض القلب الاقفاري. ويعتبر من الامراض الشائعة واحد الاسباب الرئيسية لحالات الوفاة في العالم. اضافة الى عوامل الخطورة التقلي | Coronary artery disease (CAD) is the end result of the accumulation of atheromatous plaque within the walls of the coronary arteries resulting in shortage of oxygen supply and ischemic heart disease (IHD). It was consider as one of the most common diseases and major causes of worldwide morbidity and mortality. In addition to traditional risk factors for cardiovascular disease, nowadays, accumulating evidence indicates that a variety of infectious agents may contribute to pathogenesis of CAD. The present study evaluated the anti - Helicobacter pylori IgG and IgA and the role of virulence factor of H. pylori cytotoxin associated gene (Cag A) and vacuolating associated cytotoxin (Vac A) as a risk factors for CAD.Eighty patients were divided into 2 groups : first group was 70 patients with CAD, the other group contained 10 patients with another coronary artery disease; they were admitted to Ibn Al - Bitar Specialist Center for Cardiac Surgery in Baghdad between October 2013 and January 2014. Ten individuals used as a healthy control group. All blood samples were tested biochemically such as glucose test, urea test, creatinine test and lipid profile test(cholesterol, triglyceride, LDL and HDL) and all of them showed normal results. The present study revealed that males were affected more than females (3 : 1); with no relation between their ages and bacterial infection.Serum IgG and IgA was Estimated by indirect immunofluorescent (IIF) whereas Cag A, Vac A and high sensitive C - reactive protein (hs - CRP) measured by enzyme linked immunosorbent assay (ELISA) and compared to control group results.The incidence of the anti - H.pylori IgG was highly significantly prevalent in CAD patients 78.57% (55/70), than in control group 0% (0/10), also a high significant difference in another CAD patient group 100%(10/10) as compared to control group 0% (0/10) (P? 0.01).Similarly anti - H. pylori IgA in CAD patients and another CAD patient groups showed highly significant increase when compared to control group 37.14% (26/70), 30% (3/10) and 0% (0/10) respectively (P? 0.01).The incidence of the CagA - positivity was significantly prevalent in mean value (2.74±0.19) in patients with CAD and another CAD patient group(2.72±0.31) than in control group (1.64± 0.16) (P? 0.05). Same result was observed with Vac A antigen; mean value of patient group showed significant increase (1.299 ± 0.04) when compared to controls group (1.41±0.13) (P? 0.05). Also significant increase found between another CAD patient group(1.79±0.17) and control group (1.41±0.13) (P? 0.05). Furthermore; the present study revealed significant differences of concentration levels between hs - CRP in CAD patient group (4.95 ± 0.38 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml), as well as a significant differences found between another CAD patient group (3.96 ± 0.96 µg/ml) as compared to control group (0.77 ± 0.06 µg/ml) (P? 0.05).

استعمال التقانة الجزيئية في تشخيص التباين الوراثي في تراكيب وراثية من الحنطة المتحملة للملوحة == Using Molecular Biology In Identification of Genetic Variation In Wheat/Genotypes For Salt Tolerance

اسم المؤلف: غفران علي حسين العبيدي
اسم المشرف: وسام مالك داود | ابراهيم اسماعيل المشهداني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: This study is made in bio - technology Research Center/Al - Nahrain University 2012 - 2013 for heredity genetic variation by using RAPD and ISSR method between tow genotypes elected to status salinity tolerance 2H and N5 and local varieties Iraq and Latifia sensitive to salinity.Tow experiments are made, first for measure the percentage of germination under conditions of salinity, being of planted the seeds of genotypes and local varieties studied in three replicates and three salt levels 0, 16, 12 ds.m - 1 by 10 seeds in each experimental unit, and after 10 - 15 days of agriculture was to estimate the percentage of germination and the second experiment to study the genetic variation between varieties tolerant and sensitive to salinity, where its seeds are planted soil saline by tow salt concentrations 0, 20 ds/m and after 20 - 25 days of germination, took samples of the leaves of plants to extract the DNA for study genetic variation using RAPD - PCR technique between varieties tolerant and sensitive to salinity.The results of the percentage of germination of the existence of significant differences between genotypes salt - tolerant 2H and N5 and cultivars comparing Iraq and Latifia sensitive to salinity, gave genotype N5 highest percentage of germination was 71%, and gave the genotype 2H germination percentage of 62% in the salt level of salt 16 ds/m, While giving classes comparison Iraq and Latifia less germination percentage reached 16.5% and 25% at the same level of salt, also gave genotypes 2H and N5 germination percentage of 66% and 75%, respectively, while giving classes comparison Iraq and Latifia germination percentage of 50% for both cultivars in the Second level of salt, It is clear from these results that the genotypes N5 and 2H elected from breeding programs and improvement are the most salt - tolerant varieties of local Iraq and Latifia sensitive to salinity at the stage of germination and which is the stage mostsensitive to salinity of the stages of growth of other, particularly in the third level of salt 16 ds/m.The results show that the interaction of RAPD - PCR using 7 primers and there are differences between genotypes N5 and 2H and local varieties Iraq and Latifia and varied this primers in terms of the number of bands and its location was the initiator of OPC - 12 is the best among the primers being of manages to show the discriminative power of through its production bands with a molecular weight 100bp in genotypes N5 and 2H under conditions of salinity just did not show this bands in the local varieties Iraq and Latifia and under the same conditions of salinity, This indicates that this band represents a source of difference between genotypes and local varieties in the degree of salinity tolerance because this band does not appear in all genotypes and cultivars studied under non - saline conditions, it may return this deals band in genotypes and N5 2H under conditions of salinity to show Jenny expression by some of the salt - tolerant gene, which leads to the appearance of this genetic variation in salinity tolerance status.Has also been used 15 primers to determine the genetic variation technique ISSR - PCR between genotypes N5 and 2H and local varieties Iraq and Latifia sensitive to salinity, appeared a number of bands general, however, these primers cannot show any special bands, and thus these primers failed to show this genetic variation between species studied.We conclude by the results that the genotypes 2H and N5 were the most tolerant to salinity at the stage of germination and who have shown a special bands under conditions of high salinity, while the local varieties are sensitive to salinity not appeared in this band under the same conditions and by this difference in the appearance of this package can We concludethat there is a genetic variation between these genotypes and local varieties this may be due to their differences in the degree of salinity disclaim.

تاثير بعض عوامل المكافحة الحيوية في بعض الجوانب الحياتية للذبابة المنزلية Musca domestica L. (Diptera : Muscidae) == Effect of Some Biological Control Agents On Some The Biological Aspects of Musca Domestica (Diptera : Muscidae)

اسم المؤلف: علي مرتضى كاظم الياسري
اسم المشرف: محمد رضا عنون الحسناوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية تقويم كفاءة بعض طرائق السيطرة الحيوية باستعمال الفطر Sorokin Metschnikoff)) Metarhizium anisopliae والبكترياBacillus thuringiensis var.israelensis ومنظم النمو Neporex Cyromazine) ) في المكافحة الحيوية للذبابة المنزلية Musca domestica | The present study aimed to evaluation of efficacy of some methods Metarhizium anisopliae Sorokin) Metschnikoff) Bacillus thuringiensis var.israelensis and Neporex) Cyromazine) from be used as biocontrol agents against (Diptera : Muscidae)Musca domestica L. The results showed the following : 1. Different concentration of Fungal suspension have been affected the life stages of Musca domestica. The mortality rate of eggs were (18.66%) at the concentration of 2 × 103 spore /ml, and increased to (36.66%) at the concentration of 2 × 106 spore /ml. The first larvae instars have showed highest mortality rate reached (83.33%) when treated with 2 × 106 spore/ml, while (56.66%) of them were dead at concentration of 2×103 spore/ml. (36.66%) of pupae of Musca domestica were dead when exposed to 2 × 106 spore /ml. The treatment with highst concentration caused high adult mortality, i.e. (96.66%) and (93.33%) for adult males and females of the Musca domestica respectively after 168 hours, while (70%, 73.33%) when treated with 2×103 spore /ml. 2. The secondary metabolities of fungi when applied, all first larvae instars of Musca domestica were dead and (96.66%) of the when tretead with concentration 100%, while (66.66%) and (63.33%) for the same larval instars at concentration 25%. 3. The result showed the different concentration of bacterial suspension affected the mortality rate of larvae of Musca domestica.The first larva instars have showed highst mortality rate which reached (76.66%) when treated with 2 × 105 spore /ml while (50%) of them where dead at concentration 2 × 102 spore /ml after 72 hours from treatment. 4. The effect of secondary metabolites of bacteria when applied, All first larva instar of Musca domestica were dead and (90.00%) when treated with concentration 100% and decreased to (73.33%) after 72 hours from treatment , the mortality rate of adult was (100%) at concentration 100% and decreased to (63.33%) after 72 hours from treatment. 5. The concentration of insect growth regulator neporex have effected , The highst mortality rate eggs was (37.00%), at the concentration 100 part per million, while (19.33%) at the concentration 25 part per million, , the first larval instars mortality at the concentration 100 part per million, was (96.66%) at the concentration 100 part per million while (66.66%) at the concentration 25 part per million.the mortality rate of pupa was (73.33%) while (30%) at the concentration 25 part per million after 72 hours from treatment

اثر التغذية الورقية بالبوتاسيوم والحديد المخلبي في نمو وحاصل الذرة الصفراء (mays L. Zea) تحت نظام الري بالتنقيط == Effect of Foliar Nutrition of Potassium And Chelated Iron In Growth And Yield of Corn (Zea Mays L.) Under Drip Irrigation System

اسم المؤلف: ايمن احمد عبد الكريم العباسي
اسم المشرف: نجم عبد الله جمعة الزبيدي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: نفذت تجربة حقلية في محطة ابحاث محاصيل الغالبية /محافظة ديالى في الموسم الخريفي 2013في تربة ذات نسجة طينية غرينية بهدف معرفة تاثير التغذية الورقية بالبوتاسيوم والحديد المخلبي في نمو وحاصل الذرة الصفراءL.) Zea mays (صنف بحوث 106 تحت نظام الري بالتنقيط بتصمي | A field experiment was conducted during autumn season 2013 in silty clay soil at Field Crops Researchs Station in AL - Ghalbiea/ Diyala Governorate.The aim of this experiment to study effect of foliar nutrition of potassium and chelated iron in growth and yield of maize(Zea mays L.) Buhooth 106 under drip irrigation system.Random Complete Block Design (RCBD) was used in this study with three replications. Spraying four concentrations of potassium(0, 1000, 2000, 3000) mgK. L - ¹ as potassium sulfate K%41.5 and four concentrations of chelated Iron EDTA(Fe%13)(0, 50, 100, 200)mg Fe.L - ¹. The results showed that the suitable) K (foliar nutrition treatment was 3000mgK.L - ¹ which caused significant differences in plant height, number of leaves, stem diameter, leaf area, dry matter of green plant, ear length, number of rows per ear, number of grain per ear, weight of 500grains, grain yield, chlorophyll, concentration of K and concentration of Fe as compared with control, it in order to195.87cm, 15.51 leaf, 27.32mm, 59.11dm2, 6017g.m2, 20.158cm, 17.025row, 516.75grain, 143.75g, 9.507t.ha - 1, 51.34 SPAD units, %3.003, 145.36mg.Kg - 1.Using Fe - foliar nutrition treatment was 50mgFe.L - ¹ caused significant differences in plant height and number of leaves it 193.76cm and 15.54 leaf، and 100mgFe.L - ¹ caused significant differences in stem diameter, leaf area, dry matter of green plant, ear length, number of rows per ear, number of grains per ear, weight of 500grains, grain yield, chlorophyll and concentration of K as compared with control it in order of 27.76mm, 60.41dm2 , 6004g.m2, 20.95cm, 17.15 row, 505.33 grain, 143.00g , 9.646 t.ha - 1, 50.08 SPAD unit, 2.9284%.While 200mgFe.l - ¹ caused low of all traits parameters except concentration of Fe in leaves was 188.18 mg.Kg - 1.The interaction between potassium and iron K3000 * Fe100 of significant effect in more traits parameters compared with control treatment and concentration of potassium decreased high level of iron(200mgFe.L - ¹) of monitor form

تعيين بعض الظروف الزرعية لانتاج انزيم السليليز والكحول الاثيلي من بعض انواع البكتريا المعزولة من التربة == Determination of Some Cultural Conditions For Cellulase And Ethanol Production By Bacteria Isolated From Soil

اسم المؤلف: عبد الستار عبد الجبار ابراهيم
اسم المشرف: عدنان نعمة عبد الرضا العزاوي | انيس عبد الله كاظم
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: The study included isolate and diagnose of some types of anaerobic and aerobic bacteria capable of decomposing and fermentation of cellulose. Isolation and diagnose the bacteria Clostridium phytofermentous from 10 agricultural soil samples, was 50 % of the total 50 colony, while isolation of Escherichia coli from 15 waste water samples which represented 40% of the total 75 colony and isolated Pseudomonas aeruginosa from 15 normal soil samples, and was 53% of the total 75 colony. production of cellulase enzymes was evaluated by using absorbance method. Higher activity obtained via Clostridium phytofermentous with maximum value of 42.8 IU / mL, while Pseudomonas aeruginosa gives 12.5 IU / mL and Escherichia coli gives 29.1 IU / mL.. Optimum temperature was 35 C and pH 7, for production cellulose enzyme The concentration of ethanol Alcohol was measured by using Titration method. Maximum concentration of ethanol alcohol produced by Escherichia coli, which reached 8.28 g/liter, while Pseudomonas aeruginosa was 7.86 g/liter, and Clostridium phytofermentous reached 6.62 g/L.

دراسة بيئية للطحالب الملتصقة على بعض النباتات المائية في مياه مبزل الفرات الشرقي (الحفار) / الديوانية - العراق == An Ecological Study of Epiphytic Algae On Some Aquatic Plants In East Euphrates Drainage Water /(Al - Haffar) Al - Diwaniya/Iraq

اسم المؤلف: زهرة كليب مهدي الخزعلي
اسم المشرف: فؤاد منحر علكم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الاحياء المائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناولت الدراسة الحالية الخصائص البيئية المؤثرة على الطحالب الملتصقة على النباتات المائية في مبزل الفرات الشرقي (الحفار) في الديوانية - العراق, اذ شملت الدراسة قياس بعض الخصائص الفيزيائية والكيميائية لمياه المبزل والخصائص الاحيائية التي تضمنت دراسة كمية ون | The present study tackles ecological characteristics which is effected of Epiphytic algae in east Euphrates drainage (AL - Haffar) in AL - Diwaniya, This study included the measurement some of physical and chemical characteristics of drainage water, and biological characteristics which includes studying quality and quantity of Epiphytic, Three aquatic plants Ceratophyllum demersum, Phragmits australis, and Imperata cylindrical had been selected, Samples collected monthly during The period from November 2012 to October 2013.Three location have been chosen, the first is near AL - Abtan village, Second is after the first location five kilometers, while the third location is after the Second about seven kilometers. The study shows that the temperature of the air is about (7 - 39c) and water temperature is approximately (10 - 33c), while light penetration is about (10.5 - 63cm) the flow speed is (0.25 - 0.68 M/Sec), Water turbidity is (40.02 - 63.03 NTU), while Electrical conductivity is approximately about (3014 - 3964 Sc/cm). The results of the study shows that the water drainage Oligohaline, the salinity values ranged from (1.92 - 2.53‰), the total (T.D.S) is about (1741 - 2226 mg/l), while the T.S.S. is about (41 - 67 mg/l). The result of this study shows that the pH is limited Through all the period of the study (6.98 - 8.18), The drainage water get good airing dissolved oxygen values are(6.71 - 8.14 mg/l), biological oxygen demand are (2.05 - 5.23mg/l) and carbon dioxide values are about (81.50 - 119.57mg/l).The results of this study also show about that drain water is alkaline and belonged to bicarbonate ions, total alkaline is (140.28 - (189.27 mg/l) It is discovered that the drain water is too hard, hardness values are (512.15 - 612.45mg/l), while Calcium concentrations are (128.36 - 173.40mg/l) and Magnesium concentrations are (30.31 - 50.09 mg/l). Plant nutrients, N concentrations are about (0. 98 - 3.99 µg/l) of Nitrit, Nitrate concentrations are (216 - 415 µg/l) and Phosphate concentrations are about (0.9 - 2.92 µg/l), and The percentage of total organic carbon in the residues is(0.59 - 0. 17) %. The total number of epiphytic algae types which are diagnosed during study period reaches to 320 species belongs to 86 genera within 7 classes They are Bacillariophyceae, Chlorophyceae, Cyanophyceae, Euglenophyceae Xanthophyceae, Dinophycea eand Rhodophyceae, Bacillariophyceae obtaints the dominion which reach to (183) species of Bacillariophyceae. It makes rate (57.19%).Than Chlorophyceae came concerning types number 68 species in rate (21.25%) , Following by Cyanophyceae 53 species in rate 16.56%. Euglenophyceae are 10 species in rate (3.13%).Xanthophyceae is represented in two species belong to2 genera.It makes proportion (0.63%) , Dinophyceae which represented in 3 species belonged to 3 genera in rate (0.94 %) and Rhodophyceae represented in one species and one genera in rate (0. 3%) of total number of algae.Class of the Bacillariophyceae, during study period were Navicula وNitzschia وCymbella وSynedra وAchnanthes, genera of Oscillatoria and genera of, Scenedesmus manifest that highest number of available types during the period of study.It has note that there are numbers of the monthly and located changes in total number of epiphytic algae on host plants.was varied in months and locations due to the host plants. Ceratophyllum demersum marks the Highest total number of epiphytic algae 172.89× 104 individual/ gm of wet weight in third location during April. Imperata cylindrica marks the lowest total number of epiphytic algae 3.51×104 individual/ gm of wet weight in scecond location during January, The total number of epiphytic on host plants ranges between (175.39 - 668.6) ×104 individual/ gm of wet weigh.The total number of main epiphytic algae classes is varied in total number of epiphytic algae.Bacillariophyceae diatoms composed 81.02%, in all locations during the study period, followed by Cyanophyceae composed 9.7%, while Chlorophyceae composed 8.6%.Euglenophyceae, Chrysophyceae, Dinophyceae and Rhodophyceae, were not important concerning their number. Statistical analysis results show there are significant differences between locations and months of study in relation to physical, chemical and biological features. Also there are connected correlations positive and negative between epiphytic algae and physical, chemical and biological features that measured during the study period of probability level of P<0.05.

دراسة مناعية لمستضد متعدد السكريد للفطر Aspergillus niger في الارنب == Immunological Study of Aspergillus Niger Polysaccharide Antigen In Rabbit

اسم المؤلف: غدير كاظم عبود الخفاجي
اسم المشرف: نداء شهاب حمد | فريال جميل عبد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: جمعت خلال هذه الدراسة 50 عينة تمثلت ب 25 عينة تربة و25 عينة قشع من اشخاص مصابين بالتدرن الرئوي والوافدين لمركز التدرن والامراض الصدرية في محافظة بابل خلال المدة المحصورة للفترة من تشرين اول 2013 ولغاية شباط 2014 تم خلالها عزل الفطر Aspergillus niger وبنس | fifty specimens were collected, 25 samples were collected from soil while the other 25 sample were collected from sputum of pateints with Tuberculosis, who admitted to thoracic diseases center in Babylon province in period October 2013 to Febreury 2014. Aspergillus niger Has been Isolated from these sample with 56% appearance in soil & 48% in sptum of TB pateints.these isolated were examined micro and macroscopically. purified Fungi was culture on potato dextrose broth, after incubation period polysaccharide antigen of A.niger cell wall was prepared for isolated from soil and TB patients suptum. Molish, protein and glucose test to provide that these antigen are polysaccharide, then immunuization of local rabbit of Orcyctalagus conninculus age 3 - 5 months & 1 - 1.5 Kg for males only (3 animals for each antigen) After the immunization period (one month) some of local and systemic immune response parameters (cellular and humoral) to test animals were studied and compared with control animals which immunized with normal saline. The results of study showed that the polysaccharide antigen isolated from these two strains lead to stimulate systemic humaral immune response which had been studied by using direct agglutination test for appendix immunoglobulin passive haemagglutination test (both were used to determined antibody titer), radialimmunodiffusion test in order to determine level of concentration of immunoglobulins (IgG, IgM) and complement part (C3, C4). where The results of direct agglutination titer of animals immunized with polysaccharide antigen 128 and 1 for control animals and heamagglutination tests titer of animals immunized with polysaccharide antigen serum (2560). These results were higher compared with control group titer (10) and this is also true for immunoglobulin and concentration of IgG, IgM for immunized animals compared to control one. In addition to that The concentration of C3 and C4 had been increased in immunized animals compared to the control. cellular immunological response had been measured by using rabbit Neutrophil Elastase Kit. The eresult show an increase in the level of phagocytosis in animals immunoized with polysaccharide antigen isolated from A.niger cell wall of TB pateints 0.37±0.03 pg/dl while decreased in animals immunoized with polysaccharide of soil A.niger 0.72294±0.25548pg/dl compared with control animals 0.6±0.16386 pg/dl. on the other hand the humaral adaptive immune response had been identified by measuring cytokine IL - 2, IL - 10 in serum rabbit, the level of IL - 2 in rabbit immunoized with polysaccharide antigen soure TB patients and soil is 5.4129±1.08979 and 4.2641±0.62997 pg/dl respectively compared with control animals 3.9327±0.55587 pg/dl. The level of IL - 10 had been dropped for immunized animals with A.niger of TB pateints and from soil 4.8762±1.15104 and 8.2632±4.31933 pg/dl while the control animals having the highest level 10.2576±5.23356 pg/dl among the groups. IgE concentration measured significant difference at p>0.05 in immunized animals of A.niger in both TB pateints and soil 1.1701±0.05811 and 1.0770±0.19355 ml/Iu compared with control where IgE0.8723±0.0849ml/Iu. Miotic index of bone morrow immunized and control animals show mean difference is signification at p>0.05.where the result demonstrate that the polysaccharide antigen fissed the bone marro cells compared to controlled one. In order to identify the viability of antigen in protection. challenge dose test made by injection animals with 108 spore/ml of A.niger conidia which prove their protection in immunized animals while the control animals undergo many morphological and behavioral changes after 5 - 7 days of their injection.I addition that the size of lung and spleen increased when compared with immunized one. The histological culture of lung and spleen of challenge test animals in order to identify the ability and speed of their body to evadicate and prevent infection, display that A.niger coloni appeared for control animals (6 rabbit) while disappeared in culture of immunized one. Skin test also performed to experimental animals which indicate that polysaccharide antigen of A.niger interact with hypersensitivity test of rabbit, where the level of late hypersenetivity increased clearly with propability of p>0.05 with the highest level after 24 hours of beginning test compared to controlled one.

دراسة بيئية وحياتية للمجتمع السمكي في نهر الفرات/ وسط العراق == Ecological and Biological Study of fish Community in Euphrates River / Middle of Iraq

اسم المؤلف: ماهر محمد خضيري
اسم المشرف: ميسون مهدي صالح الطائي | مؤيد جاسم ياس العماري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية في نهر الفرات عند سدة الهندية خلال المدة من تشرين الاول 2012 لغاية ايلول 2013 لغرض دراسة بيئة وحياتية المجتمع السمكي، وتم اختيار موقعين، يقع الموقع الاول في شمال سدة الهندية، ويقع الموقع الثاني في جنوب سدة الهندية، وتضمنت الدراسة تاث | The Present study was carried out on the Euphrates River during the period from October 2012 to September 2013 to study ecology and biology of fish community in the river, two study sites were chosen the first one was in the north and the second one was in the south of Al - Hindiya dam.The study was included the effects of some physical, chemical factors of water and Al - Hindiya dam on ecological and biological of fish population as well as measurement of the concentrations of some heavy metals (Pb, Cd, Mn, Zn) in both dissolved and particulate phases of water and in the tissue of three types of freshwater fish that was high dominance in three age groups. Study of the number of species, individuals, Total of weight of individual. Some ecological indices were study as index of Relative abundance, Species Richness index and Shanon wiener index for determining the evaluate of biodiversity of fish. Also Jaccared index of similarity was used to explain the similarity between sites of study and among months. Constant index was used to determine the constancy of taxa. Biological study of this of three fish that have high dominance from the side of food, age, growth, sex ratio, gonado somatic index and length - weight relationships. Air temperature and water temperature were ranged in two study sites between (15 - 43.8) C? and (10 - 29) C? respectively, pH values were between (7.6 - 8.6), Water current velocity values were between (0.14 - 0.72) m/sec, light penetration values were between (27.3 - 193.3) cm, the value of electrical conductivity were between (822 - 1323) ? sem/cm , Total dissolved solid values were between (663.3 - 932) mg/L, while The total suspended solid were between (0.009 - 0.23) mg/L and the values of dissolved oxygen were ranged between (6.13 - 10.33) mg/L.The biological oxygen demand were recorded values that range between (0.68 - 4.3) mg/L. . The water of Euphrates river were considered slight alkaline according to the results of present study and ranged between (33.33 - 164.3) mg CaCO3/L and the water were very hard according to high values record for total hardness (391.6 - 886.6) mg CaCO3 /L, while the values of calcium and magnesium hardness were ranged between (86.1 - 188.4), (19.8 - 138) mg CaCO3/L respectively.The values of phosphate were ranged between (0.51 - 13.12) ?g/L and Nitrate values were ranged between (6.23 - 70.17) ?g/L, while the values of nitrite were ranged between (0.17 - 4.98) ?g/L. in especially, the effects of Al - Hindiya dam, the results were showed that numbers of species before and after of Al - Hindiya dam were ranged between (23 - 17) species respectively. the results were showed positive and negative correlation between physical, chemical properties of water and Heavy metals with number of species, individuals, weights and Biodiversity indices. This study showed that the mean concentrations of the heavy metals (Pb, Cd, Mn, Zn) of the dissolved phase in water were (2.14, 1.61, 3.10, 7.63) ?g/L respectively and in the particulate phase were (33.99 112.87، 84.21، 21.12،) ?g/g dry weight respectively, the results of present study were showed that the concentration of heavy metals in water in dissolved and particulate phases within local and world limitations. The heavy metals in muscular tissue for three groups of this fish were ordered in as following : Zn >Mn > Pb > Cd. Twenty - three fish species were collected, (17) of them belonged to Cyprinidae family and two species belonged Bagridae family while only one species represented each family of Mugilidae, Siluridae, Mastacembelidae, Cichlidae, the number of species were ranged during the period of study between (5 - 18), The total number of individuals was 2401 individual and the number of individual were ranged monthly between (129 - 276) individual, while the total weight of individuals were 232868 gm during the period of study and The weight of individuals were ranged monthly between (13072 - 29598) gm. Carassius auratus wae recorded with the highest percentage in number and weight as 35.2% and 27.5% respectively. while A.vorax was after C.auratus in total number 13.6%, but it was after C.auratus and C.carpio in total weight 16.9% of the total individual weight. The values of species richness index were ranged between (0.79 - 3.23) and numerical of shanon wiener index values (Hn) were ranged between (1.26 - 2.39), while the weight of shanon wiener index value (Hb) were ranged between (1.21 - 2.35). the numerical and weight of eveness index (Jn) were ranged between (0.65 - 0.86), (0.68 - 0.89) respectively during the period of study. Jaccared similarity index were ranged between (23% - 92%). And the study was revealed that the most species recorded during the period of study were constant species according to constancy index. C. carpio fish were Omniovorous and It's feeding activity was ranged between (70% - 91.42%) and feeding intensity was (6.9 - 12.17) degree/fish.while C. auratus were detritious, It's feeding activity was ranged between (56.75% - 94.28%) and feeding intensity was (6.91 - 12.87) degree/fish. A. vorax were carnivorous and It's feeding activity values were ranged between (69.2% - 90.32%), while feeding intensity values were ranged between (6.85 - 13.03) degree/fish. The results was showed the values of age and length for C. carpio that ranged between (1 - 5) years, (9.7 - 40.2) cm , and C. auratus were ranged between (1 - 6) years, (8.3 - 28) cm, while A.vorax were ranged between (1 - 7) years, (10 - 62) cm respectively. Sex ratio and gonado - somatic index (GSI) varied between the three species, sex ratio of C.carpio (male : female) was 1 : 3.1. (GSI) for male was ranged between (2.21 - 12.02) and for female between (3.36 - 19.36).The sex ratio of C. auratus male : female was 1 : 1.9, GSI values for male was ranged between (0.89 - 8.53) and for females between (1.23 - 61.37). A.vorax sex ratio male : female 1 : 1.5, GSI value for male and female were ranged between (0.36 - 2.84) and (0.24 - 5.83) respectively.Variation of values of slope factor (b) were calculated from length - weight relationships for three species of fish, the values of (b) factor for C.carpio, C.auratus and A. vorax (3.08, 2.97, 2.92) respectively during the period of study

تشخيص طفيلي المشعرات المهبلية بتقنية تفاعل البلمرة المتسلسل RT - PCR في محافظة واسط - العراق == Diagnosis of Trichomonas Vaginalis Using Real - Time Pcr Technique In Wasit Province - Iraq

اسم المؤلف: رسل واسط كاظم
اسم المشرف: عبد السادة عبد العباس راهي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: واسط
الصفحات الاولى:
المستخلص: في الدراسة الحالية، نصف طريقة سلسلة تفاعل البلمرة المتسلسل للكشف عن DNA محدد من طفيلي المشعرات المهبلية في الفحص السريع لتقنية الوقت الحقيقي لسلسلة تفاعل البلمرة. وتقييم مدى انتشار داء المشعرات وفقا مع دراسة العوامل المختلفة التي تؤثر على داء المشعرات الم | This study was involved the describe of an existing PCR method for specific detection of Tichomonas vaginalis DNA into a rapid real - time PCR assay, and to evaluated the recent prevalence of trichomoniasis accordance with studying different factors that affect on vaginal trichomoniasis in females complaining of vaginal discharge.Vaginal swab samples were collected from 401 patients attending two public hospitals and three private clinic in the Al - Kut city. Demographic data, clinical and socioeconomic status were collected from the patients using a structured questionnaire. Total genomic DNA was isolated from 60 samples of vaginal swab randomly chosen to specific detection of T. vaginalis DNA into a rapid real - time PCR.All samples were examined by the direct microscopic examination (wet mount and Giemsa - stained smear). Their ages were ranging from 14 - 63 years. The vaginal swabs examined by wet mount and Real Time - PCR to detect the presence of T. vaginalis. The pH strips were used to detect the vaginal acidity.Of the 60 women, 13 were positive by Real Time - PCR giving the prevalence rate of (21 67%) with sensitivity, specificity and accurate rate 100% and the direct microscopic examination (wet mount and Giemsa - stained smear) show 5/60 (8.33%) positive case while 55/60 (91.67%) gave a negative result with a sensitivity and specificity, and accuracy (38.5%) (100%) (86.7%).Women aged (24 - 33)years had significantly higher prevalence of trichomoniasis (46.1%) than other age groups.Married women had the highest percentage of trichomoniasis (76.9%), comparing with widowed women (7.7%) and divorced women (15.4). With statistical significant difference between these groups. Uneducated women had significantly higher rate of T.vaginalis infection (76.9%) than educated women (23.1%). Statistical significant difference was detected between these groups.Pregnant women showed a higher infection rate 7(53.8%) than non - pregnant 6 (46.2%).Women with vaginal pH (6 - 7), (5 - 6) had significantly higher rate of infection (53.8% and 38.5%) respectively.Women who used (loop and the oral contraceptive pills) contraception had the lower rate of infection(23.1%) than those not using any contraception (67.9%). Statistical significant difference was detected between these two groups.The highest percentage 2(66.67%) was found among women with T. vaginalis who used contraception for >2 years.The higher percentage of infected with T. vaginalis parasite was found in bad odor or fish odor vaginal discharge which was noted (76.9%) of infected women with T. vaginalis. Statistical significant difference was detected between these groups. Women with Frothy discharge color showed higher significantly percentage of infection 7(53.8%) than other with Yellow to green and bloody discharge and Clear discharge 3(23.1%), 2(15.4%) and 1(7.7) respectively.Multipara women revealed the highest rate of trichomoniasis (46.15%). No statistical significant difference was detected between infection and parity.T.vaginalis was more commonly seen in women with frequency of sexual intercourse 2 - 3 time per week 5 (50%) cases. The lowest incidence was detected among those who had sexual intercourse once per month 1 (10%), with statistical significance among these variables. T.vaginalis was found to be predominant among housewives (69.23%) than employees (30.77%).Fertilizing women were showed higher 9(69.2%) T. vaginalis infection than non - fertile women 4(30.8%).The high incidence of T.vaginalis infection was found among rural females 8 (61.5%), followed by urbanized females 5 (38.5%).

دراسة تصنيفية لعويلة الخنافس البرغوثية Alticinaeمن عائلة Chrysomelidae ورتبة غمدية الاجنحة Coleoptera في بعض محافظات العراق == Taxonomic Study On The Flea Beetles (Coleoptera : Chrysomelidae : Alticinae) In Some Provinces of Iraq

اسم المؤلف: رغد عـبيد خضير
اسم المشرف: سعدون ابراهيم اسماعيل
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تناول البحث لهذه الرسالة دراسة تصنيفية لعويلة الخنافس البرغوثية Alticinae في بعض محافظات العراق، علما ان هذه العويلة لم تدرس تصنيفيا في العراق.اختير النموذج Longitarsus ballotae Marsham. لتجسيد صفات العويلة. ولقد درست الاجزاء الرئيسة للجسم وهي الراس والص | The Subject of thesis is a taxonomic study of Subfamily Alticinae in some provinces of Iraq. Knowing that this Subfamily has not been studied taxonomically in Iraq.Was chosen as the model for Longitarsus ballotae Marsham. To embody the gualities of the Subfamily. The detailed study of the head, thorax, abdomen and their appendages was made to evaluate the reliability of the external characters in classifying the species of the Subfamily.There are eight genera and thirteen species for these Subfamily The species are : - Altica deserticola Weise.Aphthona fuentei Reitter.Aphthona sp. Chaetocnema montenegrina Heikertinger.Chaetocnema persica Baly. Epitrix atropae Foudras.Hermaeophaga ruficollis Lucas.Longitarsus ballotae Marsham.Longitarsus membranaceus Foudras.Longitarsus reichei Allard. Longitarsus succineus Foudras.Phyllotreta nemorum Linnaeus.Podagrica fuscicornis Linnaeus.6 Species Register in Iraq are : - Chaetocnema montenegrina Heikertinger.Epitrix atropae Foudras. Longitarsus succineus Foudras.Longitaarsus membranaceus Foudras.Podagrica fuscicornis Linnaeus.Two species Taxonomic keys were designed and developed to is olate the genera of Subfamily Alticinae, sa wellas the genera mentioned and studied in this thesis for each species.

الخصائص التشريحية والتصنيفية لانواع مختارة من ذوات الفلقتين البرية النامية في محافظة ديالى == Anatomical And Taxonomical Attributes of Wild Dicots Selected Spesies In Diyala Pravince

اسم المؤلف: ضفاف خليل سلام البدري
اسم المشرف: علي حسين عيسى الموسوي | محمود شاكر رشيد الجبوري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة تشريحية مقارنة لعشرة انواع مختارة من العائلات المختلفة لذوات الفلقتين. النامية في مناطق الصدور والسوامرة وطريق العظيم، التابعة لمحافظة ديالى، والانواع هي : - Centaurea bruguieriana (Dc.)Hand.Mzt. 1.Onopordon acanthium Eig. 2.Ero | Ten wild selected species belong to different Dicots families were anatomically comparative studied. These species grown wildly in Sudoor, Suwamra, and Kallis _Udaim way of Diyala province, as follows : Centaurea bruguieriana (Dc.)Hand.Mzt. 1.Onopordon acanthium Eig. 2.Erodium glaucophyllum (L.) L' Her. 3.Eruca sativa Mill. 4.Matthiola longipetala (Vent.)Dc. 5.Psylliostachys spicata (willd.) Neaski 6.Ranunculus cornutus DC. 7.Rumex cyprius Murb. 8.Scabiosa palaestina L. 9.Verbena officianlis L. 10. The research concentrated on the anatomical comparative characters for the first time in Iraq. These characters regarded as a Diagnostics for these species. Epidermis with its variable cells and stomatal complexes were investigated, so as for venation in leaves, mesophyll and vascular bundles. According to the above characters the species were divided in to groups. This work was determined the distinguish characters of the petiols, midribs of leaves, in addition to the anatomical information of stemes, roots and different kinds of indumentum and glands which were aided to separate these species. The variable anatomical results were important and were assisted the morphological ones. Field photographs for the specimens, schedules were given, and the results were also discussed according to the environments of the Taxa studied.

دراسة نسجية مقارنة بين عضلات الصدر والارجل في نوعين من الطيور الطائرة وغير الطائرة في عمرين مختلفين == Comparative Histological Study Between Pectoralis And Leg Muscles In Flying And Running/Walking Birds In Two Different Ages

اسم المؤلف: تغريد حامد عبد الامير
اسم المشرف: هاشم محمد عبد الكريم العلاق
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناولت هذه الدراسة عددا من العضلات الهيكلية في نوعين من الطيور الطائرة (الحمام) وغير الطائرة (الدجاج). العدد الكلي للطيور المستعملة 30 طائرا, 15 من الدجاج و15 من الحمام تمت دراسة بعض الخصائص الفسلجية والنسيجية للالياف العضلية الحمر والبيض والوسطية وحساب | This study deals with a number of skeletal muscles in two types of birds ; flying (pigeon) and running (chicken) types. The total number of the studied birds was 30; 15 were chicken and 15 were pigeons. Some of the physiological and histological characteristics of the muscle fibers (red, white, and intermediate) , the percentage of fibers diameter in the studied muscles, and the change noted in the muscle weight with advancing age had been calculated. The species of the chicken in this study was Gallus domesticus , while the species of the pigeons was Columba livia domestica. The muscles that is used in the study were : pectoralis muscle, femortibialis, triceps, biceps brachii, extensor carpi radialis muscle, and gastrocnemius muscles in two age groups of birds; (3 - 6) months and (6 - 12) months in order to know the changes in the weights, numbers, diameters, and sizes of the muscle fibers that occur with advancing age. The aim of this study is to identify the three types of the muscle fibers their role in the muscle movement and the regions in which there is specific type rather than others. The results had shown that the skeletal muscles in birds consist of heterogenous mixture of the three types of muscle fibers (red, white, and intermediate) , and the white muscle fibers are the predominant type in the pectoralis muscle of both types of birds, and the size of white muscle fibers in the pectoralis muscle in the chickens in the first age group was 73.8%, and in the second age group was 82.9%. While in pigeons, the size of white muscle fibers was 75.5% in the first age group, and 75.4% in the second age group. In addition, the present study deals with the relationship between the muscles weights and the age of the bird in that the muscles show increased weight with advancing age in both types of birds and there were significant differences in muscles weight in both age groups (p?0.05), while the triceps muscle did not show significant difference regarding weight between the two age groups in the same type of birds. The results of the study also show the differences in the diameter of the muscle fibers, that is there was continuous increase in the diameters with advancing age and show significant differences (p?0.05) , but regarding pectoralis muscle there was no significant differences in diameters with advancing age in both types of birds. The results of the study also show that the pectoralis muscle was the largest muscle in the body of the bird, and the ratio of its weight to the total body weight was relatively large.

تاثير الري بالماء المعالج مغناطيسيا في نمو وحاصل الحنطة Triticum aestivum L == Effect of Irrigation With Magnetic Treated Water On Growth And Yield of Wheat (Triticum Aestivum L.)

اسم المؤلف: فاضل كاظم كريم الابراهيمي
اسم المشرف: ثامر خضير مرزة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: نفذت التجربة في حقل تابع للمعهد الفني - الكوفة شمال غرب محافظة النجف لموسم النمو (2012 - 2013) بهدف معرفة تاثير نوعية مياه الري وشدة معالجتها مغناطيسيا لثلاثة اصناف من حنطة الخبز Triticum aestivum L. في مؤشرات النمو الخضري والزهري والكيميائي والانتاجي.ص | An experiment was conducted in a field belonging to the Technical Institute - Kufa north west of Najaf governorate during the season of the year (2012 - 2013) to determine the effect of irrigation water quality and intensity of magnetically treated to three cultivars of bread wheat (Triticum aestivum L.) on vegetative growth, flowering, chemical and productive indicators.The experiment was designed randomized complete blocks (RCBD) arrangement Split split - plots and three replicatesfor each treatment. Experiment included study three factors is the quality of irrigation water (river water or well water) - Main plot, wheat bread varieties (IPAA - 99, Rashid and Tamuze - 2) - Sub - plot and magnetic treatment to irrigation water four intensities is (0, 750, 1500 and 3000) Gauss - Sub - sub - plot. The Revised Least Significant Difference (RLSD) at 0.05 probability was used to compare treatment means, whenever treatments affects were evident.Results showed : 1 - Wheat plants irrigated with river water were superior in all parameters in forms of plant height, leaves number, leaf area, tillers number; productive and non - productive, root length, lateral root length of the plant, leaves content from total chlorophyll, dry weights for plant vegetative, phosphorus% in grains, grains weight of spike, spike number of plant and m2, weight of 1000 grain, plant yield of grains, m2 yield of grains, hectare yield of grains per hectare compare to plants irrigated with well water, which recorded the highest chemical content of grains from nitrogen, potassium, magnesium, protein and carbohydrates percentages. The percentage of flowering, spikes length, spikes grains number were superior in the beginning with the well water, were superior at the end with river water.2 - Magnetic treatment with irrigation water by 1500 gauss revealed that noticeable superior was obtained in most of plants vegetative and floral characteristics, while the chemical characteristics had increased with magnetic water treatment by 3000 gauss and yield characteristics treated with 750 gauss. The effect of magnetic water treatment on leaves content from total chlorophyll and magnesium percentage in grains was negative.3 - The majority of vegetative, chemical and productivity parameters was increased with Rashid plants cultivar compared to same parameters for Tamuze - 2 plants cultivar which superior than IPAA - 99 plants cultivar except number of leaves, tillers number and spike grains number which superior with IPAA - 99 plants cultivar.4 - The triple interaction between experiment all factors revealed a significant effect on magnetized river water at 1500 gauss in Rashid and Tamuz - 2 plants cultivars giving highest vegetative growth indicators average which increased on plant yield with low magnetic water treatment (750 gauss) to Rashid plants cultivars and medium strongly (1500 gauss) for Tamuz - 2 plants cultivars, while the characteristics of grains chemical content were superior with same plants cultivars but magnetized treatment well water at 3000 gauss.

دراسة تشريحية لبشرة اوراق بعض اجناس العائلة المركبة Compositae (Asteraceae) في جامعة القادسية == Anatomical Study of The Leaves Some Genus Compositae (Asteraceae) In Al - Qadisiyah University

اسم المؤلف: فرقد حيدر عبد النبي المحنة
اسم المشرف: ازهار عبد الامير سوسة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناول البحث الحالي دراسة تشريحية مقارنة لصفات البشرة للاوراق بعض الاجناس التابعة للعائلة المركبة (Compositae) وهذه الاجناس هي Aster L. و.Calendula L وDendranthema L. و.Helianthus L و.Lactuca L و.Launaea L و.Tagetes L, وتبين ان لبعض الصفات التشريحية اهمية | The present research includes comparative anatomical study epidermis of some genera which belong to sun flower family (Compositae) These genera are Aster L., Calendula L., Dendranthema L., Helianthus L., Lactuca L., Launaea L. and Tagetes L. , and show that details anatomical the importance of taxonomic possible from isolation genuses more each adjective undulate the walls vertical epidermis the leaves, and we show some importand for indumentum suchas T - shape eglandular haire in Dendranthema L. species

التحري عن جينات المقاومة لبعض المضادات في Candida spp. المسببة لداء المبيضات == Detection On Resistant Genes To Some Antifungal In Candida Spp. That Causing Candidiasis

اسم المؤلف: رجاء علي حبيب ظاهر الخزاعي
اسم المشرف: علي حمود السعدي | نيران عبيد جاسم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تم جمع 107 عينة من حالات سريرية مختلفة شملت 36 مسحة فموية من اطفال مصابين بالسلاق الفموي, 32 مسحة مهبلية من النساء المصابات بداء المبيضات المهبلي و39 عينة بولية من اشخاص مصابين بالتهاب المجاري البولية (راجعوا مستشفى الديوانية التعليمي العام ومستشفى النسائ | The present study was aimed to determine ERG11 - 2 that responsible for resistance to some antifungal agents in candida spp. Atotal of 107 samples were obtained fromdifferent clinical cases included(36 oral swabs from children suffering from oral thrushwho attending Al - Dewaniya Maternity and Pediatrics Teaching Hospital in AL - Diwaniya, 32 vaginal swabs from women suffering from vaginal candidiasis who attending clinics in AL - Diwaniya, 39 urine samples from individuals suffering from urinary tract infection who attending Al - Dewaniya Maternity and Pediatrics Teaching Hospital in AL - Diwaniya) and 25 samples from sewage water as a control samples in the molecular study.The result of morphological and biochemical tests showed For pathogenic samples that 83(77.5%) isolate of candida spp. Included 36/31(86.1%) from oral samples, 32/28 (75%) from vaginal swabs, 28/39 isolates (71.7%) from urine samples while the result of non - pathogenic samples revealed that 20 (80%) isolate of candida spp. Detecting of, ERG11 - 2 resistant gene was done by RealTime - PCR technique. Results showed that all isolates 31 (100%) were carrying ERG11 - 2 that responsibe of resistance in candida to some antifungal, Did not record any isolated from control samples from sewage water which amounted to (9) the ERG11 - 2 resistant

دراسة جرثومتي السالمونيلا والشيكلا المعزولة من حالات سريرية في مدينة الديوانية وتاثيرهما على معايير دم المرضى

اسم المؤلف: هبة اركان فنطيل الحسناوي
اسم المشرف: علي عبد رحيم الناشي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: Salmonella is a bacteria and Shigella types of intestinal bacteria, and because of the seriousness of the pathogenicity and the spread of infection in their environment has been conducting this study.Collected during the current study, 462 different clinical sample (feces and urine) of adults aged 20 years and older and those with symptoms of the bacteria Salmonella and Shigella and both sexes reviewers for laboratories and hospitals in the city of Diwaniya, for the period from 2012 \ 11 \ 6 to 2013 \ 5 \ 26. Diagnosed isolates based on phenotypic traits and AGRO tests biochemical in addition to diagnosis by Api20E system as isolates Salmonella reached 17 isolation and by 3.6% and were all belonging to the type S.typhi, either isolates bacteria Shigella was three isolates and 0.64%, and all of them belong to the type of Sh. Flexneri. The incidence of bacteria S.typhi for the male sex was higher than in females, Ozbulg number of infected males and 10 by 58.8%, while the number Fayalnads was infected 7 and by 41.1%, but for bacteria to Sh. flexneri, as was the incidence in males is also higher than in females as in males reached 2 and by 66.6%, while the number of infected females in bringing only one injury and 33.3%. Were more age groups injury is a class 20 - 29 where recorded 9 injuries and by 52.9% and the number of infected males 6 The number of cases in females 3 injuries, age group 30 - 39 recorded 5 injuries and by 29.4 and were two goals in the male and three injuries in females, the age category 40 - 49sgelt two goals by 11.9% and the injury was confined to males either category above 50 years and one injury recorded only in females and 5.8%, either in bacteria Sh. flexneri was the only injury in the 20 - 29 age group and by three injuries. The more isolated the proportion of bacteria S.typhi in the month of May and April as recorded 4 isolates each, and by 23.5%, while giving the lowest ratio for the presence of bacterial during the months of December and January, as recorded isolation and one each of these two months and 5.8% either November remaining months February, March was the number of isolates in 3, 2, 2, respectively, and 15%, 17.6%, 11.7, 11.7%, respectively, as well, either for the bacteria Sh. flexneri as Bulgtaaly proportion of injury in the month of May was the number of isolates 2 and by 66.6% during the month of April either recorded a hit single Vqtobnsph 33.3% did not notice any injury to the other months of the study. Tested the sensitivity of the isolates S.typhi and Sh.flexneri direction antibiotics used as 8 used antibiotics in common use in this study show that the bacteria were sensitive S.typhi 100% Anti Ciprofloxacin, but resisted antibiotics Ampicillin, Amoxicillin, Chloramphenicol, Tetracycline, Nalidixicacid, Gentamicin, Ceftriaxone at a rate of 82.3%, 82.3%, 76.4%, 70.5%, 64.7%, 23.5%, 23.5% respectively. The bacteria Sh.flexneri was 100% sensitive to antibiotics Ciprofloxacin, Gentamicin, Nalidixic acid, Amoxicillin, Ceftriaxone while it was resistant to antibiotics Tetracycline, Chloramphenicol, Ampicillin by 66.6% each. Been investigating some of the virulence of the bacteria S.typhi factors, including the presence of the portfolio Capsule as were all Salmonella isolates characterized by possessing the portfolio, while the production of the enzyme protease Protase was all isolates S.typhi producing this enzyme also showed 12 isolation of the ability to produce the enzyme Lipase and increased by 70.5%, but all isolates were S.typhi unproductive enzyme protease. The bacteria Sh.flexneri marked by Azltan of three Azladtha and by (% 66.6) viability link red dye Congo also showed 2 of these isolates and by (66.6) ability to form thin membrane (Pellicle) and two Aezzltan which have demonstrated susceptibility to adhesion was isolated one of them only possess cilia first pattern type 1. The research covered also study the changes that occur in some of the blood parameters in people infected with the bacterium Salmonella Heclauzar that there is a decrease in the total number of red blood cells Red blood cells in people infected with Salmonella, where the total number of blood cells Reds reached 4.91 x 106 c \ ml total number of cells rate 3.7 white blood x 103c \ ml the proportion of types of white blood cells of the total number of cells, the white was lymphocyte ratio lymphocytes% 52.55 which is low compared with the normal number, while there was an increase in both acidophilus cells Eosinophils and only Monocyte as she was represented 1.2%, 2.9% in November while it was% 0.03 Basophils cells, the highest percentage of increase in healthy people, and the amount of total hemoglobin rate. Hemoglobin9.07 g \ dl which is low compared with healthy peopleBut in people infected with bacteria Sh. Flexner, bringing the average number of red blood cells 106 c \ ml3.7 x, the lowest rate with the number of healthy people, as the amount of hemoglobin g \ dl13.5 rate, either the total number of white blood cells, the rate was slightly higher than what is found in healthy people Azbulg (11.32 x 103c \ ml), it was noted that most of the increase was high in the white blood cells of the type Neutrophils the increase amounted to 62.34%

دراسة التاثير الاليلوباثي لنبات اليوكالبتوس Eucalyptus sargentii في انبات ونمو بعض نباتات الزينة == The Allelopathic Effect of Eucalyptus Sargentii On Germination And Growth of Some Ornamental Plants

اسم المؤلف: كوثر هاشم عبار الجاسمي
اسم المشرف: مجيد كاظم عباس الحمزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت تجربتين, حقلية ومختبرية, لدراسة التاثير الاليلوباثي لمسحوق الاوراق الجافة او الطرية لنبات اليوكالبتوس ومستخلصاتها (المستخلص الايثانولي, الزيت, التربينات, القلويدات والفينولات) في الانبات وصفات النمو الخضري والزهري ومحتوى بعض العناصر المعدنية, السكري | Two experiments were carried out, field and laboratory experiment, to study the allelopathic effect of dry or fresh leaves and their extracts of Eucalyptus sp. on four ornamental plants; Calendula officinalis, Tagetes patula, Mathiola incana and Asteriscus graveolens Less. The two experiments were conducted at the field of College of Agriculture/Al - Qadisiya University during the period from 31/10/2012 to 14/3/2013. For the field experiment, dry and fresh leaves of Eucalyptus were used at the rate of 0, 2, 3, or 6 g dry leaves/Kg soil or at the rate of 0, 4, 8, or 12g fresh leaves/kg soil. Plant height, changes in height during plant life time - course, number of branches, number of flowers, and flower diameter were measured. In addition, chlorophyll content in leaves, anthocyanin in petals, dry weight, nitrogen percent, potassium and phosphorous content in leaves were also determined. For the laboratory experiment, ethanol extract, oil, terpenes, alkaloid, and phenols extracted from Eucalyptus leaves at different concentrations were used. Seed germination, coefficient velocity of germination, plumule and radical length, sugar, protein, melanodialdehyde, glutathione and proline content were evaluated. The results were as follow : 1. Ethanolic extract was negatively affect the percent and coefficient velocity of germination in addition to plumule and radicle length. The reduction in these parameters was greater at the highest concentration of the extract, especially in Calendula officinalis. 2. Treatment with oil extracted from Eucalyptus leaves caused a significant reduction in percent and coefficient velocity of germination, plumule and radical length. 3. Terpenes, alkaloids or phenols treatments at different concentrations resulted in significant reduction in percent and coefficient velocity of germination, plumule and radical length. The reduction was increased as the concentration of the treatments increased. 4. Plant height decreased significantly due to the treatment with dry or fresh Eucalyptus leaves, the reduction in plant height increased as the concentration of the treatment increases in all plants except Asteriscus graveolens plant which showed that the small concentrations of both treatments. It was close to ward in an increase in plant height. 5. Number of branches also significantly decreased when ornamental plants treated with dry or fresh Eucalyptus leaves; the Calendula officinalis plant was the most affected. 6. For the changes in ornamental plants height, as a result of treatment with dry or fresh leaves of Eucalyptus, during life time - course, it was clear the growth curve was close to the S - shape growth curve with obvious significant differences for most treatments compare to control. 7. Dry weight percent was significantly affected in all ornamental plants when they treated with dry or fresh Eucalyptus leaves, If decreased with the concentration of the treatments increased. 8. It was obvious that treatment with dry or fresh Eucalyptus leaves resulted in significant reduction in flower number and flower diameter. Number of flowers decreased as the concentration of the treatments increased. on the other hand, flower was diameter increased as the concentration of the treatments increased, but in both cases it was less than the control. All one obvious that reducing significant in flower diameter, It was reducing great in low levels. 9. Chlorophyll content was also decreased in leaves, while anthocyanin content in petals was increased when the ornamental plants treated with dry or fresh Eucalyptus leaves. 10. Nirogen percent, phosphorus and potassium content in leaves of the four ornamentals decreased when they treated with dry or fresh Eucalyptus leaves. 11. Using terpenes, alkaloids and phenols caused significant increase in sugar content in leaves of the ornamentals, reaching its highest values at the highest concentration of the terpenes. For protein content, it was gradually decreased as the terpens, alkaloids and phenols concentrations increased in Calendula officinalis. In Tagetes patula, protein content was noticeably increased with the increasing in concentrations of the treatments. 12. Malondialdehyde, glutathione and proline content increased significantly in Calendula officinalis and Tagetes patula plants when they treated with terpenes, alkaloids or phenols. 13. Interaction between treatments concentrations and kind of plants had significant effect on all parameters measured.

دراسة تركيبة الهائمات النباتية وعلاقتها بالظروف البيئية وبعض العناصر الثقيلة في مياه نهر الديوانية - العراق == A Study of Structural Phytoplankton And Their Relationship With The Environmental Conditions And Some Heavy Metals In Al - Diwaniya River /Iraq

اسم المؤلف: نور ناظم جابر الميالي
اسم المشرف: فؤاد منحر علكم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تناولت الدراسة تركيبة الهائمات النباتية وعلاقتها بالظروف البيئية وبعض العناصر النزرة في مياه نهر الديوانية, اذ جمعت عينات الدراسة (المياه والرواسب والهائمات النباتية) شهريا من ثلاث محطات على نهر الديوانية ابتداء من تشرين الاول 2012 ولغاية ايلول 2013، وشمل | This investigation was designated to study of structural of phytoplankton and its relationship with environmental conditions and some of heavy metal in AL - Diwaniya river, the samples were collected monthly for water, sediments and phytoplankton from October 2012 till September 2013, the study included the measurement of the temperature of air and water; Light penetration; salinity ;electrical conductivity ; T.S.S;T.D.S ;Turbidity; pH ;Oxygen Dissolved; Biological Oxygen Demand ;Chemical Oxygen demand ;Dioxide carbon ;AL - alkalinity; Hardness; Ca; Mg; and nutrients(nitrate; nitrite; phosphate) ; total organic carbon) and the study also included the measurement of some heavy metals (Cadmium, lead, copper, Zinc) in dissolved and particular parts of water and in sediment both exchangeable and residual parts and also studied the structure of phytoplankton quantity and quality.Results of the study showed that the temperature of air and water ranged between (15 - 43) ?C (13 - 33)?C respectively. It was found the light penetration ranged from (27.2 - 100), conductivity was (1264 - 1555)?S/cm, while the salinity was (0.80 - 0.99)%, pH was ranged between (7.37 - 7.82), while Turbidity was ranged between(43.6 - 59.02) NTU, total dissolve substances was (985 - 1411) mg/L while total suspend solid substances was (40 - 66) mg/l. The dissolved Oxygen was within the range of (6.3 - 8.26) mg/l, the biochemical and chemical Oxygen demand was (1.19 - 3.08) mg/l (12.4 - 38.01) mg/l respectively while dioxide carbon was ranged(80.5 - 134.2)mg/l.The study revealed that the river water was alkalinity and very hard, as the range of total alkalinity, total hardness, Calcium and magnesium was (131.5 - 227.4, 312.3 - 458.5, 86.4 - 142, 54.8 - 76.8) mg/ respectively, while the concentrations of the nutrients were showed fluctuated in concentrations, it was (102.6 - 558.4, 1.5 - 3.4, ND - 2.9) ?g/ for Nitrate, Nitrite and Phosphate respectively, total organic carbon was between (0.13 - 0.55) %Seven units were diagnosed during the period of study Bacillriophyceae; Chlorophyceae; Cyanophyceae; Euglenophycae; Dinophycae; Chrysophycae ;Cryptophyceae.Species number of phytoplankton that identified through study period were (134, 101, 96) in three sites respectively, and showed that dominance of Bacillriophyceae (diatoms) which was (71, 50, 46) species fallowed by Chlorophyceae (35, 25, 25) species then Cyanophycae (22, 18, 20) species, Euglenophycae (2, 5, 2) species and Dinophycae(2, 3, 1) species Chrysophycae (1, 0, 2) and finally Cryptophyceae (1, 0, 1)species in three sites respectively,. Generally the most common genera in study period are (Nitzschia, Surirella, Nivicula, Navicula, Syndra, Pediastrum, Scenedesmus, Aphanocapsa, Microcystis, Cocconeis, ,).The total number of phytoplankton was low and ranged between (422.21 - 1027.82) cell ?103/l. This study determined means of heavy metals (Cd, Zn, pb, Cu) in dissolved part was (ND - 5, 4.07 - 29.8 ND - 6.89, 2.08 - 8. 71) ?g/l respectively, while particulate part was) 2.09 - 30.16, 44.87 - 372.17, 3.13 - 90.93, 27.41 - 88.20) ?g/g respectively The study revealed also that the concentrations of heavy metals in sedimentswas (ND - 12.71, 11.76 - 39.93, 0.55 - 6.12, 9.31 - 39.73), ?g/g of exchangeable parts and was (1.09 - 17.94, 39.21 - 172.30, 21.21 - 67.96, 33.96 - 76.63) ?g/g of residual parts respectively. The Statistical results showed there are morale differences between locations and seasons in relation to physical and chemical features, organic compounds, heavy metals and phytoplankton. There are negative and positive, morale correlations between phytoplankton and physical, chemical features and heavy metals that measured during study period at probability at the level of (p<0.05).

تاثير الادينوسين ثلاثي الفوسفات على اخصاب وتطور الاجنة الاولي خارج الجسم الحي في الفئران كنموذج للجنس البشري == Effect of Adenosine Triphosphate On In Vitro Fertilization And Early Embryonic Development In Mice As A Model For Human Being

اسم المؤلف: مهند علي مشكور
اسم المشرف: محمد باقر محمد رشاد فخر الدين | نوال خيري العاني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الاجنة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يعد الاخصاب خارج الجسم الحي (IVF) من احد اهم التقنيات المستخدمة لانتاج اعداد كبيرة من الاجنة لبحوث التطور الاساسيه والبيولوجيا الجزيئية لنضوج البويضات المختبري والاخصاب. يعتمد الاخصاب خارج الجسم الحي وظروف حفظ الجنين المثلى على مكونات الوسط الزرعي كونها ا | In vitro fertilization (IVF) is one of the most important techniques used to produce large numbers of embryos for research into the basic development and molecular biology of in vitro oocytes maturation and fertilization. Optimal IVF and embryo culture conditions depend on the composition of culture media being the most important determinants of success in vitro interaction of the gametes and subsequent embryo development. Adenosine 5 - triphosphate (ATP) is a fundamental factor to maintain life, by providing energy, and controlling the cell function and metabolism. Objectives : The present study aims to investigate the effect of ATP supplied to SMART medium on a rate of fertilization and early embryonic development (ED) in mice as a model for human beings.Materials and Methods : The female mice (90) were superovulated using superovulation program to produce a large number of oocytes. These were divided into three groups, the first group was inseminated with vassal sperm in vitro alone (control); the seconed group (G2) was inseminated with vassal sperm in vitro using SMART medium enriched with low concentration of ATP(1.25mM). The third group (G3) was inseminated with vassal sperm in vitro using SMART medium enriched with high concentration of ATP (2.5mM). Mouse spermatozoa were collected from both vasdeferens. Then sperm parameters were assessed after 30 min. IVF technique was performed for 3 groups, and assessed after 20 - 22h. Then, percentages of early embryonic development stages and abnormal embryos morphology (%) for each embryo stage were assessed.Results : Results of the present study revealed significant increment (P<0.05) in the in - vitro fertilization (%) when using 1.25mM ATP as compared to 2.5 mM ATP and the control group. Significant differences (P<0.05) in the 1 - cell stage were assessed in 2.5mM compared with 1.25mM groups and the control group. Also, Significant differences (P<0.05) in the 2 - cell and 4 - cell stage were assessed in 1.25mM ATP compared with other groups. However, non significant differences in the percentages of abnormal embryos morphology were reported between both treated groups of ATP.Conclusion : From the results of present study it was concluded that the low ATP concentration (1.25mM) enriched to the culture medium improved the percentage of in vitro fertilization and early embryonic development.

تقييم التعبير الجيني للجينات CK19, MGB, MUC1 microRNA - 195 and microRNA - let 7a في نساء عراقيات مصابات بسرطان الثدي == Evaluation of MGB1, CK19, MUC1, microRNA - 195 and microRNA - let - 7a Expression In Iraqi Women With Breast Cancer

اسم المؤلف: جودت نوري غائب
اسم المشرف: عبد الحسين مويت الفيصل
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The present study aimed to shed light on the identification a panel of genes with distinct expression patterns in breast cancer patients as a useful tool for breast cancer early detection and progression. The present study designed to investigate the levels of genes expression of five genes panel (MGB1, CK19, MUC1, miR - let7a, and miR - 195) in circulating free mRNA and miRNA from blood of breast cancer patients versus noncancerous samples (benign tumor and healthy controls) to establish a biomarker panel potentially useful for early detection and progression of disease. The expression patterns of the identified genes were then compared with certain clinical features (age, lymph node status, and tumour size).Blood samples from 55 patients with different stages of newly diagnosed invasive ductal carcinoma were provided by certain Iraqi hospitals. Two control groups were used in this study; 10 samples of patients with benign breast tumors, and 20 samples from healthy donors. The samples preservation with TRIzol was done. Samples subjected to total RNA extraction, and then molecular study by using reverse transcription and real time PCR at Molecular Oncology Unit in Guy´s Hospital - Kings College / London.The study reached the following results : 1. The patients’ age range was 24 - 70 years and the median was 49 years with high frequency of patients in the range of 40 - 59 years. According to the family history, 50(90.91%) of patients were have negative family history. According to the clincopathological features (lymph node status and tumor size) the percentages of patients with multiple lymph nodes and tumor size 2.0 - 2.9 cm were the highest groups, which showed statistically highsignificant differences.2. For MGB1 gene expression, the result showed that 30(54.5%) patients were MGB1 - positive while 25(45.4%) patients were MGB1 - negative.According to malignancy status the percentage of patients with high level of MGB1 gene expression 22(40%) was significantly high. In correlation with age groups there was statistically no significant differences in the levels of gene expression with age. In correlation with clincopathological features, lymph node status showed that the highest percentage of MGB1 positive patients 18(66%) were multiple for lymph node status, and the tumor size results showed that there was decreasing in the MGB1 geneexpression with increasing of tumor size. 3. For CK19 the results of present study showed that 41(74.54%) patients were CK19 - positive, while 14(25.46%) patients were CK19 - negative.According to malignancy status the percentage of patients with high level of CK19 gene expression 30(54.45%) was significantly higher in compare with benign tumor patients and healthy controls. In correlation with age groups there was statistically no significant differences in the levels of gene expression with age. In correlation to the clincopathological features, lymph node status results showed that the highest percentage of CK19 positive patients 24(88.89%) were multiple for lymph node status, and there was increasing in the CK19 gene expression with increasing of tumor size.4. Mucin 1 (MUC1) gene expression results showed that the percentage of MUC1 - positive breast cancer patients 72.73%(n=40) was significantly higher when compared with benign tumor patients and healthy controls. According to the age groups the results showed no significant correlation with patients age groups. The clincopathological features results showed that the highest percentage of MUC1 positive patients 84.21%(n=16) have few lymph node status, and there was statistically significant association between the increasing of MUC1 gene expression and tumor size. 5. The miR - 195 gene expression results showed that the percentage of patients with positive miR - 195 gene expression 83.64%(n=46) was significantly higher than patients with negative miR - 195 expression 16.36%, the study also showed that the percentage of high miR - 195 expression samples 69.09% (n = 38) was significantly higher in compare with benign tumor patients and healthy controls. According to the clincopathological features, patients with multiple and few lymph node metastasis were found to have significantly the highest percentages of miR - 195 expression, while the tumor size results showed that there was increasing in the miR - 195 gene expression with increasing of tumor size. 6. The percentage of miR - let 7a - positive breast cancer patients 81.82% was significantly higher, when compared with miR - let 7a - negative patients 18.18%. In correlation to the clincopathological features, results showed no significant correlation in miR - let7a gene expression levels with patients age groups, for lymph node status, the results showed that the highest percentages of let 7a positive patients were those with multiple lymph node and few lymph node metastasis. The tumor size results showed that there was increasing in miR - let 7a gene expression with increasing of tumor size.7. According to genes combinations, three genes combination (CK19, miR - 195 and miR - let 7a) was significantly positively expressed with percentage of 60%(33/55), which reflect their potential diagnostic and prognostic value.8. The study concluded that the three genes combination (CK19, miR - 195 and miR - let 7a) may have potential applications as a diagnostic and prognostic markers for breast cancer.

دراسة جزيئية، وخلوية وراثية، ومناعية لمرض سرطان القولون والمستقيم في العراق == Molecular, Cytogenetic And Immunological Study of Colorectal Cancer In Iraqi Patients

اسم المؤلف: حيدر جاسم محمد الخفاجي
اسم المشرف: علي حمود السعدي | عزام قنبر اغا
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: The study consists of three parts related with patients of CRC as in : 1 - Molecular part 2 - Cytogenetic and molecular genetics part and 3 - Immunological part. In addition of their relatives of clinical and pathological diagnosis, genus, and tumor locations in 150 of Iraqi patients confirmed with CRC collected from Teaching Hospitals of some Iraqi governorates. The percent of male to female (54.66% / 45.43%) and the aged range of study were 16 to 80 years.The first part consist a study of five types of genes related with CRC represented by MLH1, MSH2, APC, K ras, and SMAD7 genes. Some of these genes develop with germline mutations as in MLH 1 exons1 and 15 so as MSH2 genes. While the rest genes related with a sporadic of CRC. All these genes were amplified by conventional polymerase chain reaction (PCR) for study the types of the mutations and their defect of these genes on CRC. The APC exon11and K ras exon1 genes were processed by single strand conformational polymorphism (SSCP) technique. The results of PCR - SSCP technique for two genes represented by appeared three types of bands, which were (AA), (AB) and (AC) bands. The important band was (AB) which constitutes the variability region represented by 84.2% and 44.82% in patients for both genes. The results of patients with mutated bands with high significant differences(P?0.05).compared with healthy control. Patients carried these bands subjected to PCR - Sequences technique. The analysis results for APC gene sequences were substitutions mutations type of single nucleotide polymorphism (SNP) change Cytocine to Thiamin (C>T) at 1972 location of genome. While the analysis results of Kras gene appeared two types of mutations, substitution and frame shifts represented by deletion mutation and so as appeared stop codon mutation.The other genes represented by MLH1, MSH2, and SMAD detected by direct PCR - Sequences. The results analysis of MLH1 gene, exon (1and 15) represented by nucleotides change with high frequencies of substitution mutation through exon1 at different locations of nucleotides. So as frame shift mutation type deletion through exon15. While for MSH2 gene, exon 6 from 6.66% represent by deletion mutation. A spread study for SMAD7 gene, exon4 were done through 30 patients average age 52 years (male to female 56.7% : 53.3%) confirmed colorectal adenocarcinoma compared with 15 healthy. A mutation of 20% of (SNPs) were identified.The second part which represented standard conventional cytogenetic and molecular genetics were done through peripheral blood culture (PBC). The study showed increasing of mitotic index (MI) in patients with significant differences (P?0.05) compared with healthy controls indicating for increasing of proliferation of the lymphocytes division. The study showed some chromosomal aberrations with significant, the majority of aberrations were higher significant in dwarf and aneuploidy chromosomes so as elongation chromosomes revealed significant differences (P?0.05) between patients and healthy. The appeared of Polyploidy chromosomes, with significant differences (P?0.05), that’s seem to be good indicator for cancer disease. The molecular genetics alterations which dependent on cytogenetic investigation were done through deletion of long arm of 5 chromosome which APC gene is located. The test elucidates the importance of inactivation (deletion) of APC gene in elicited of colorectal cancer. The study of 20 patients, were investigated by fluorescent in situ hybridization (FISH). The results showed 60% patients which have 5q arm deletion of (+). And (5%) have two deletions (++) which was a male aged less than 60 years. The results analysis explained significant differences (p?0.01) between studied group and deletion (+) of arm 5q. A correlation represented by significant differences (p?0.01)through dysfunction of APC gene deletion of 5q were found with high percent among sporadic adenocarcinoma 58.3%, with less in attenuated familial adenomatous polyposis (AFAP) and familial adenomatous polyposis (FAP) represented by the (25%), and (16.7) respectively.The last part related with immunological test were done by detection of tumor markers represented by Carcino Embryonic Antigen (CEA), IL - 33 and IL - 31.These markers represented a confirmative indicators for colorectal adenocarcinoma which were tested by ELISA technique in (79) patients confirmed with CRC of male and female. These tumor markers in tested with patients showed high significant differences (p?0.01) compared with healthy controls. Another immunological test for detection of Human papilloma virus (HPV) type 18 E6 gene expression in CRC by Immune histochemistry technique (IHC) in 71 (FFPET) of CRC. The results of this study showed there were a correlations between patients and adenocarcinoma positive HPV18 E6 infection 43.7% of patients infected with virus, with high significant differences (p?0.01) and higher infection in female (67.7%) than male (32.3%). So as a correlations were found between HPV18 E6 expression and tumor tissue locations, explained by high percentage 45.2% in rectum.While a tumor marker was (CD8+) was used to determine its role in human immune surveillance in tumor regions of CRC so as with regions which were infected by HPV. The study revealed high significant differences between healthy control and studied groups which have (CD8+) positive infiltration in tumor origins through detection by (IHC).In conclusions diagnosis of CRC can be detected by special genes like K ras gene through professional PCR processing, compact with tumor markers, so as Fluorescent in situ hybridization (FISH) technique provides a precise method which can be used for detection of alteration of molecular and cytogenetic related with CRC.

تاثير بعض المستخلصات النباتية في معايير بيولوجية مختلفة للفئران == Effect of Some Plant Extracts On Different Biological Parameters On Mice

اسم المؤلف: عقيل حيدر عطا الله
اسم المشرف: مؤيد صبري شوكت
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية للتحري عن المركبات النشطة الموجودة في المستخلص الخام المائي والميثانولي لاوراق الاس والنعناع والريحان ودراسة تاثيرها على فعالية انزيم الاسيتل كولين استريز ومستوى الدهون ومستوى السكر ومستوى انزيمات الكبد(ALP وSGPT وSGOT) ومستوى الانتر | The present study was conducted to investigate the active constituents found in aqueous and methanolic crude leaf extracts of Myrtus communis, Mentha piperita and Ocimum basilicum, and studies it effect on the acetylcholinesterase activity, levels of lipids, level of glucose, level of liver enzymes (ALP, SGPT and SGOT) and level of Interleukins (IL - 2, IL - 4, IL - 6 and IL - 10) in laboratory mice (in vivo). The results of the phytochemical analysis of the crude aqueous and methanolic extracts of Myrtus communis, Mentha piperita and Ocimum basilicum contain active compounds : Phenols, Flavonoids and Tannins and missing of Steroids and Coumarines in all extract but Saponins and Alkaloids found in alcoholic extract only, while terpens were present in Mentha piperita and Ocimum basilicum and absent in Myrtus communis. The results of administrating animals with different extracts showed no significant difference on blood Acetylcholinesterase (AchE) compared with ethanol liquid diet, while the alcoholic and aqueous extracts of M. communis, M. piperita and O. basilicum in the serum of decreased Acetylcholinesterase level significantly(p?0.05), liver and brain [(1.25 ?pH/30 min, 1.23 ?pH/30 min, 1.28 ?pH/30 min, 1.20 ?pH/30 min, 1.26 ?pH/30 min, 1.28 ?pH/30 min), (0.35 ?pH/30 min, 0.34 ?pH/30 min, 0.34?pH/30 min, 0.36?pH/30 min, 0.42?pH/30 min, 0.39?pH/30 min), (0.32?pH/30 min, 0.37?pH/30 min, 0.39?pH/30 min, 0.36?pH/30 min, 0.34?pH/30 min, 0.37?pH/30 min)] respectively compared with animals fed on ethanol liquid diet [(1.37 ?pH/30 min), (0.47 ?pH/30 min), (0.45 ?pH/30 min)] respectively. The methanolic and aqueous extracts of M. communis, M. piperita and O. basilicum reported a significant decrease in level of the total cholesterol and Low Density Lipoprotein (LDL) [(181mg/dl, 186mg/dl, 175mg/dl, 172mg/dl, 181mg/dl, 184mg/dl), (118mg/dl, 121mg/dl, 114mg/dl, 109mg/dl, 118mg/dl and 120mg/dl)] respectively, when compared with animals fed on ethanol liquid diet [(195 mg/dl), (132mg/dl) ] respectively while no effect was reported on High Density lipoprotein. The level of triglyceride reduced after the treatment with ethanol liquid diet, and then the level increased after the treatment with M. communis and O. basilicum extracts. The methanolic and aqueous extracts of M. communis, M. piperita and O. basilicum have led to a reduction in the level of glucose in the serum which increased after the treatment with ethanol liquid diet. Methanolic and aqueous extracts decreased the liver enzymes (ALP, SGPT and SGOT) significantly to the normal level (18, 17, 21, 18, 20 and 21) KAU, (19, 19, 16, 13, 17, 17) IU/L and (21, 24, 17, 15, 17 and 19) IU/L respectively after the increase by the treatment with ethanol liquid diet (24) KAU, (26) IU/L, (28) IU/L as compared to control (20) KAU, (12.2) IU/L, (13.5) IU/L, respectively. The level of Interleukin - 2 and Interleukin - 4 in the serum significantly increased in the treatment of alcoholic and aqueous extract of M. communis, M. piperita and O. basilicum [(18 pg /ml, 17 pg /ml, 20 pg /ml, 18 pg /ml, 20 pg /ml, 20 pg /ml), (100 pg/ml, 110 pg/ml, 119 pg/ml, 108 pg/ml, 90 pg/ml, 92pg/ml)] in comparison with ethanol liquid diet treatment [(14 pg /ml), (77 pg/ml)] respectively. While the level of Interleukin - 6 and Interleukin - 10 increased significantly in the serum, when animals were fed with ethanol liquid diet [(259pg/ml) and (501pg/ml)] respectively, and then decreased significantly after the treatment of methanolic, aqueous extract of M. communis, M. piperita and O. basilicum reported [(198 pg/ml, 202pg/ml, 202pg/ml, 201pg/ml, 214pg/ml, 217pg/ml), (370 pg/ml, 385pg/ml, 200pg/ml, 280pg/ml, 350pg/ml and 350pg/ml)] respectively.

التاثيرات المعدلة - مناعيا للمعززات الحيويه ضد بكتريا Salmonella enterica Serovar Typhimurium المعزولة من حالات الاسهال لدى الاطفال في ذكور الفار الابيض == Immunomodulatory Effects of Probiotics On Salmonella Enterica Serovar Typhimurium Isolated From Diarrheal Children In Albino Male Mice

اسم المؤلف: صفا خليل ابراهيم
اسم المشرف: عبد الواحد باقر الشيباني | علي حسين ادحيه
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study aimed to determine the frequency of Salmonella Typhimurium and its multi - drug resistance (MDR) status as a diarrheal causative pathogen in children younger than five years old, as well as to investigate the role of four probiotic microorganisms(L.acidophillus, L.casei, S.cerevisiae, S.boulardi) in controlling such infection.For this purpose, 128 stool samples of patients (76 males and 52 females) was included in this study. They were children suffering from diarrhea and fever who admitted to the “Central Pediatric Hospital” in Baghdad during the period from April to September, 2012. Their ages ranged from six days up to five years.The immunological effects of probiotics and MDR S. Typhimurium isolate in the experimentally infected and probiotic - treated mice were investigated via assessing the level of ten cytokines (IFN - ?, IL - 1?, IL - 4, IL - 10, IL - 12, IL - 17A, IL - 21, GM - CSF, RANTES and IP - 10) in the lavage of small intestine. Accordingly, five groups of mice were used in the in vivo part of this study; Mice in group I received probiotic for 7 successive days, challenged with S. Typhimurium on day 8, and dissected on days 14 and 21. Group II wassimilarly treated, but the probiotic was continued for 14 days. Group III was given the probiotic only, and group IV was challenged with S. Typhimurium, while group V was the control.Results obtained in this study could be summarized as follows : 1. Out of 128 stool samples, S. Typhimurium was isolated and identified in 9 samples only (7.03%). All isolates were totally resistant to nalidixic acid, with the exception of one isolate which showed intermediate sensitivity.Furthermore, only one of these isolates (symbolized B) was found to be resistant to three antibiotics (ampicillin, amoxicillin and nalidixic acid).Therefore, this isolate was considered as an MDR isolate and selected for further experiments in the study.2. When the susceptibility of MDR S. Typhimurium isolate B was further assessed in vitro by using four probiotics (Saccharomyces cerevisiae, S. boulardi, Lactobacillus acidophilus and L. casei), results showed that S.cerevisiae and L. acidophilus were the most efficient by recording the highest inhibition zones (12.6 and 16.3 mm, respectively), therefore, they were further investigated for their anti - S. Typhimurium effects in vitro and in vivo. 3. By using the unconcentrated and (one - fold, two - fold, three - fold) concentrated filtrates of these two probiotics, it was found that the three - fold filtrates were most efficient in their antibacterial activity by recording the highest inhibition zones (25.0 mm for S. cerevisiae and 31.0 mm for L. acidophilus.4. Significant increases in the values of liver index were observed in mice of group I treated with L. acidophilus at 21 days (10.73%) compared to the corresponding group treated with S. cerevisiae (7.41%) or other four groups. For spleen, index value in L. acidophilus groups was higher than the corresponding groups in S. cerevisiae. Mice treated with a probiotic alone or in a combination with the pathogen showed significant increases in the spleen index values of all groups compared to the untreated mice (group V).5. Both probiotics were effective in reducing S. Typhimurium colony forming units per plate (cfu/plate) in the liver and spleen. In liver, mice in group IV showed a count of 224.4 cfu/plate, which was significantly higher than any count in groups of L. acidophilus and S. cerevisiae. Group II mice recorded better results than group I mice, while the lowest counts (21.6 and 27.8 cfu/plate for L. acidophilus and S. cerevisiae, respectively), were observed at day 21.6. The ten investigated cytokines showed different levels in the small intestine wash; such differences were subjected to the group of mice underinvestigation and type of probiotics used. In addition, variations in IFN - ?/IL - 4, IFN - ?/IL - 10, IL - 4/IL - 10 and IL - 17A/IL - 10 were also observed.

تقييم فعالية المستخلص المائي والكحولي لنبات الصبار Aloevera في الاصابة التجريبية لطفيلي Eimeria tenella لدى فروج اللحم == Evaluationof Alcoholic Andwateryextracts Efficacyofaloe Veraon Experimental Infectedbroilerwitheimeriatenella

اسم المؤلف: زهراء حاتم حميدي عبود الكعبي
اسم المشرف: هيثم محمد حمادي العوادي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على (216) فرخ دجاج نوع Rose 308مصاب بطفيلي Emiria tenella خلال المدة من شهر نيسان 2013 الى شهر تشرين الاول 2013, وقد تم في الدراسة الحالية تحديد بعـــــــــض المؤشــــــــــــــرات الدمــــوية (الخلايا المتغايرة /الخلايا اللمفاوية HL | This study was conducted to (216) broilers chickens (Rose 308 Trade) infected parasite with Eimeria tenella performed form April 2013 till October 2013, has been in the current study to identify some indicators of blood(Heterophils/ Lymphocytes, White Blood Cell, Red Blood Cell, Packed Cell Volume, Hemoglobin) and biochemistry (Glucose, cholesterol and total protein) and productive performance (Weight increase, Food conservation, Cansumbtion and Body weight befor slugtery) , We note at the parasite has infected chicks were used three extracts of Aloe vera (alcoholic and hot water and cold water), dosage chicks were to demonstrate the effect of different concentrations of these extracts on the productivity and physiological traits mentioned above. Then the experiment was designed, which include the following : 1 - The first group (control group) included 36 chicks and was given food with no anti - coccidial. 2 - The second group include 36 chicks in duplicate was infected but did not treated.3 - The third group inclouded 36 chicks in duplicate was given extract of Aloe vera Alcoholic ethyl in adose of (0.5 - 1.5 - 2)gm per mm body weight.4 - The fourth group inclouded 36 chicks in duplicate was given extract of Aloe vera Cold water in adose of (0.5 - 1.5 - 2)gm per mm body weight.5 - The fifth group inclouded 36 chicks in duplicate was given extract of Aloe vera Hot water in adose of (0.5 - 1.5 - 2)gm per mm body weight.6 - The sixth group include 36 chicks in duplicate was given Vetacox(trade Mark)that had both Sulphadimidin 80 gm and Diverdin8 gm.Results showed the following : - 1 - The alcoholic extract of Aloe vera at concentration (0.5, 1.5, 2) g/ml after an infection in the fifth week revealed alittle effect on the H/L when compared with eighth week of infection. also this extract lead to increase, the rate of (PCV), (Hb) , and (RBC) while lead to decrease the rate of (WBC) after eighth week of infection at concentration (2)g/ml. Also this extract lead to increase the rate of cholesterol, total protein and the rate of weight increased in the eighth week, while the amount of feed intake and feed conversion showed alittle effect, as well as the body weight befors slaughter has astrong effect at concentration(2)g/ml in the eighth week of infection. 2 - The cold and hot aquatic extract of Aloe vera at concentration(2)g/ml lead to decrease the ratio of H/L and increase the rate of (PCV), (Hb) and (RBC) after the infection in the eighth week, also lead to decreas the rate of (WBC) and increase the rate of cholesterol, total protein, the rate of weight increased, the amount of feed intake and the body weight before slaughter at concentration (2)g/ml in the eighth week of infection. Also recorded abetter efficiency of feed conrersion at concentration (2)g/ml in the eighth week of infection.

دراسة بيئية للطحالب الهائمة والملتصقة على النباتات المائية في نهر العباسية/ وسط العراق == An Ecological Study of Phytoplankton And Epiphytic Algae On Aquatic Macrophyta In Al - Abbasiya River/Middle of Iraq

اسم المؤلف: شيماء جبار هادي الجحيشي
اسم المشرف: احمد عبيس مطر | جاسم محمد سلمان
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية شهريا في اربعة مواقع على نهر العباسية وسط العراق للمدة من اذار 2012 الى شباط 2013، شملت الدراسة قياس بعض الصفات الفيزياوية والكيميائية لمياه النهر ودراسة الصفات النوعية والكمية لمجتمع الطحالب الهائمة (Phytoplankton) والطحالب الملتصقة | The present study was conducted monthly at four sites that located at Al - Abbasiya River middle of Iraq, for the period from March, 2012 to February, 2013 including studying some physiochemical properties of water and quantitative and qualitative of phytoplankton and epiphytic algae on aquatic macrophyta by selecting five species of aquatic plants : Phragmites australis Cav., Typha domengensis per., Ceratophyllum demersum L., Potomogeton pectinatus L. and Myrophyllum verticillatum L. Results also showed that, the air temperature ranged between 10.0 to 45.3 C?, while, the water temperature was 6.3 to 33.0 C?. Transparency values ranged between 0.29 to 3.06 m, while, the current velocity was 60 - 730 cm/ sec. The study also revealed that the pH was limited at the period of study ranged 6.97 to 9.13. Electrical conductivity and salinity were about 163.33 to 1157 ?s. cm and 3.68 to 23.0 0 ‰, respectively. Total dissolved solids values were about 277 mg/L to 900g/L. Total suspended solids values ranged between 0.09 - 37.0 mg/l. Results showed that, slightly alkaline at and bicarbonate ions prevailed, total alkalinity was 42 to 810 mg /L. Dissolved Oxygen values were 1.83 to 12.33 mg/L and biochemical oxygen demand was 1.4 to 8.0 mg/L, water hardness value in the studied sites ranged between 60 to 2000 mg CaCO3/L., While, calcium concentrations was 86.84 to 1783.0 mg CaCO3/L. and Magnesium concentrations was 6.70 to 268.66 mg CaCO3/L. Reactive phosphate concentrations in this study produced values ` ranged from 0.01 to 5.06 mg /L, and Nitrite concentrations ranged between nill to 5.20 mg /L, Nitrate concentration ranged between 0.09 to 6.47 mg /L. 199 species were recorded as total species number of phytoplankton at the period of study that, belong to Diatoms algae consisting of 61.30% : 88 species which belong to Pennales and 34 species for centrales, followed by Chlorophyta and Cyanophyta that were 42 and 21 species, respectively. Pyrrophyta 1, Euglenophyta 2, Rhodophyta 2, Pheophyta 5, Chrysophyta 5 species. Bacillariophyta gave the highest total number was 9303 × 104 to 1074 × 104 cell / L of fresh weight that was recorded at site 1 during April , 2012 and May, 2012, while, others algae 9823× 104cell/L in site 3 in July, 2012 compared to 1036 × 104cell/l at site 4 in April, 2012 for the total phytoplankton population density. Site 1 was the most divers sites with numerous number of species of algae, reaching the number of 66 genera and 85 species. while, the least number of species at site 3 were 65 genera and 70 species. April and October, 2012 have the most total cell numbers available for diatoms and non - diatoms algae. Nitzschia palea ; N.commnis and N. kutzingiana were the highest abundance of species showed an average of 46.80% of the overall phytoplankton sampled. Nitzschia was followed by Ankistrdesmus falcatus (25.53%) and Anabaena cylindrica (17.02%).There were some species present in all sites during the period from March, 2012 to February, 2013 i.e. Anabaena cylindrical, Dimorphococcus lanatus, Draparnaldia judayi, Euastrum dubium, Pilayella littoralis, Chrysoamoeba radiansklebs, Melosira distance, Stephanopyxis turris, Amphora ovalis, Cocconeis placentula, Gomphonema augur, G.tenuirostrum, Semiorbis sp and Pinularia biceps. Epiphytic algae types were diagnosed during the study of 256 species within 8 division. Bacillariophyta division was the dominate reaching 172 species composed to 67.18% of the total number, most of them belong to Pennales with 140 species that was more than Centrales 32 species, Chlorophyta 47 species form 18.35%, Cyanophyta 22 species form 8.59% and Chrysophyta and Pheophyta 5 species for both formed 1.95% , Euglenophyta and Rhodophyta 2 species for both formed 0.78% and Pyrrophyta algae that represented by one species and one genus. The highest genera number during the study period were Cymatopleura, Eunotia, Nitizschia, Cymbella and Gomphonema. Numbers of epiphytic algae varied in months and sites due to the host plants. The highest total number was 31039 ×104 cell/ L of fresh weight was recorded at site 1 during April, 2012 on leaves of Ceratophyllum demersum plant, while, the lowest total number was103×104cell/L of fresh weight which was recorded at site. 2 during June, 2012 on shoot of Potomogeton pectinatus. Many species of epiphytic algae were different among the study sites; the highest number was 148 species in Ceratophyllum demersum recorded 93 species on the shoot and 110 species on the leaves, while, the lowest number was 148 species on P. pectinatus recorded 36 on the root 36 and 52 species on the shoot and 60 species on the leaves. The result of study confirm that some of epiphytic algae were found at the most plant parts and different hosts, such as Anabaena cylindrical A. diolum, Lyngba aestuarii, Microcystis aeruginosa, Cosmacladium tubetculatum, Scenedesmus quadricauda, Palmodictyon, Mougeeotia, Pithophora oedogonia. Results showed that, some species favor some parts of host plant e.g Anabaena cylindrica was the most dominated on the Myrophyllum verticillatum and P.australis.Schizothrix tinctoria was dominated on the shoot of Phragmites australis while, algae Nostic linkia dominated on the shoot of Typha domengensis. Algae Ankistrodesmum falcatus, Pyramimonas tetrarhnchus ; Tetraspora cylindrical were dominated on the shoot of Potomogeton pectinatus. There were only seven diatom taxa common encountered on all plant part e.g. Cyclotella bodanicavar var. michiganensis on the shoot of Myrophyllum verticillatum. Fragilaraia capucina var. mesolepta, Cocconeis placentula and Achanthes affinis were dominated on the shoot of Phragmites australis, it was also found that, Navicula miniscula present on leaves of Potomogeton pectinatus and Ceratophyllum verticillatum. Algae N. notha and N. odioas were found on Myrophyllum verticillatum leaves. Ten species were recorded for the first time at this study in Iraq from phytoplankton and epiphytic algae from the period of March, 2012 to February, 2013 : one species of Cyanophyta epiphytic algae Anacystis nidulans ;one species of chlorophyta epiphytic Trentepohlia aurea ; four species of phytoplankton Bacillariophyta centrales : Melosira jurgensi; M. undulata ; Stephanopyxis turris ; Thalassiosira anguste - lineata ; T.decipiens and four species of epiphytic Baciliariophyta pennales : Achanthes flexella; Amphora bullatoides ; Asterionella formosa ; Navicula notha.Five diversity indices : relative abundance, Species richness; Shanon - Weiner diversity ; Jaccard similarity index and Sorinson similarity index were calculated in this study. Relative abundance index for phytoplankton was 44.5 at site 3 in April, 2012 while, epiphytic algae recorded the highest value was 91.6 on the shoot of C.demersum at site 1 in February, 2013 ; on the roots and leaves of T.domengensis in sites 2 and 3 were 66.6 in March, 2012 and 36.36 in June, 2012 on the leaves the same of plant. The highest value of species richness of phytoplankton was 21.9 at site 3 in April , 2012while, for epiphytic algae was 18.3 on the leaves of M.verticillatum and 9.0 on shoot of P.pectinatus at site 4 in March, 2012; on the leaves of C.desmersmum was 12.3 at site 2 ; on the shoot of M.verticillatum plant was the highest value at site 1 on May and June, 2012, while, on leaves and shoot of P. australis plants was the highest value in May and July, 2012 at site 2 and on root of P. australis and shoot of P. pectinatus at site 4 on August, 2012and February, 2013 at site 3, respectively. Results cleared a similarity with high value of Jaccard and Sorenson indices ranged between 3.2 to 50.0 and 55.87 to 153.21 respectively, while, the value of Shanon - wiener diversity index was ranged between 0 - 18.3 as high value which were 4.6 of M. verticillatum plant leaves at site 1 for May and June, 2012 and at low value during the period of study at sites 2, 3 and 4 for most plant parts. A statistical analysis was done using the canonical correspondence analysis(CCA).

دراسة عدد من ملوثات الهواء وتاثيراتها البيئية في منطقتين مختارة من جانب الرصافة في بغداد == Study of Several Air Pollutants And Their Environmental Effects In Two Regions In Al - Rusafa Site In Baghdad

اسم المؤلف: شيماء هادي حمادي الدليمي
اسم المشرف: محمد نافع علي العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Several air pollutant concentrations such as Suspended Particulate Matter (SPM), carbon oxides (COx), sulfur dioxide (SO2), nitrogen oxides (NOx), methane (CH4), ozone (O3) and some heavy metals such as lead (Pb) , chromium (Cr), cadmium (Cd), and nickel (Ni). Were measured in selected areas of the Rusafa in Baghdad, Anda’lus square (as a commercial site) and Wazeria district (as industrial site).The results showed that the concentration of air pollutants from gases, SPM (NO2, CO, SO2, and SPM) and heavy metals (Pb, Cr, Cd, and Ni) were exceeded globally and locally permissible limits in both study sites, while the concentration of O3, CO2 were within the globally and locally standard limits in both study sites. Commercial site had recorded higher concentrations of (CO2, SPM, Pb) than those of industrial site. While the industrial site had more pollution with (NO, NO2, CO, CH4, Cr). Results showed a relationship between climate factors and air pollutants, where windspeed, temperature, and relative humidity had influenced on the concentration of air pollutants.Median Lethal Concentration (LC50) to nitrogen dioxide gas was identified, by exposing a community of 30 adult male mice. The mice weights were between 23 - 31gm and their age was 8 - 12 weeks. The mice were randomly divided into five groups (6 male of each group). The exposure periods were 20, 40, 60, 80, 100 minutes using different concentrations 50, 100, 150, 200 ppm.The results showed a higher mortality during 150 and 200 ppm concentrations, for exposure periods of 80 and 100 minutes, but other concentrations have not been recorded. LC50 was calculated 161.86 ppm for 80 minutes and 110.53 ppm for 100 minutes.

دراسة التاثيرات الحادة والمزمنة للمبيد العشبي - 4, 2 دايكلوروفينوكسي اسيتك اسد في نوعين من اسماك الكارب == Study The Acute And Chronic Effects of The Herbicide 2, 4 - Dichlorophenoxy Acetic Acid In Two Species of Carp Fish

اسم المؤلف: ضحى زكي السويفي
اسم المشرف: احمد جاسم محمد العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية التاثيرات السمية الحادة والمزمنة لمبيد 2, 4 - d في نوعين من اسماك الكارب (الاعتيادي والعشبي)، استعمل في دراسة التاثيرات الحادة عدة لكلا النوعين قيد الدراسة وكانت ((170, 165, 160, 155, 145, 140, 130 و(140, 130, 115, 95, 70)ملغم/لتر ع | This study included acute and chronic effects of 2, 4 - d pesticide in two species of carp fish (Cyprinus carpio and Ctenopharyngodon idella) using several concentrations for the two carp fish species that were (130, 140, 145, 155, 160, 165, 170) and (70, 95, 115, 130, 140) mg/l respectively for 96 hours, to determine the median lethal concentration (LC50) and the behavioral changes was used as a standard of those effects. Whereas three concentrations (20, 40 and 60) and (10, 15, 25) mg/l respectively for the two carp species were used in the chronic exposure for six weeks. The behavioral, histological and hematological changes were used as a standard to observe these effects through the study period. The value of LC50 for common carp fish were 157.77, 157.25, 156.41 and 152.62 mg/l. For grass carp, LC50 were 115.92, 114.92, 112.94 and 96.52 mg/l for 24, 48, 72, 96 hrs of exposure to 2, 4 - d pesticide concentrations respectively.Behavioral changes were recorded more effects in high concentrations than in low concentrations. To discover the toxic effects of this substance on fish, many parameters were used. They included the clinical symptoms by erratic swimming, hyperactivity, loss of equilibrium with respiratory effects such as rapid gill movement and swam near the water surface. While in chronic effects, blood parameters were changed in Red Blood Cell R.B.C., White Blood Cell W.B.C., Packed Cell Volume P.C.V., Haemoglobin Hb and liver enzyme (GPT & GOT).Values elevated significantly according to the pesticide concentration (P?0.001) and exposure periods (P?0.05) in comparison with control. The histological examination of the gills, liver and kidney of the common and grass carp fish after chronic exposure to 2, 4 - d herbicide showed pathological changes and alterations such as fusion of the secondary lamella, epithelial lifting, blood congestion, epithelial hypertrophy of the lamellar epithelium and epithelial necrosis. In the liver, pathological changes were seen, including hepatocytes hypertrophy, cytoplasmic vacuolation, blood congestion and cellular necrosis. Degenerative changes of the renal epithelium, necrosis in renal tubules and occlusion of the tubular lumen where the pathological alterations in the kidney.

تطبيق بعض طرق حفظ الحشرات على حشرة المن == Applicating of Some Methods of Maintainance On Aphids

اسم المؤلف: ضياء عباس عبد الرضا الزيادي
اسم المشرف: عبد المحسن حسين مؤنس
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: دبلوم عالي
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تعد الحشرات ذات اهمية اقتصادية وطبية وجمالية مما دعت بالباحثين الى دراستها من كافة الجوانب الفسلجية واطوار حياتها وتكيفاتها ومناطق استيطانها وطرق تغذيتها للوصول الى حلول كفيلة للحد من الاضرار المسببة عن الحشرات الاقتصادية والطبية من جانب ولتكثير وتربية ال

تاثير الماء المعالج مغناطيسيا في بعض الصفات المظهرية والكيموحيوية في عقل نبات الماش (Phaseolus aureus Roxb.) == Effect of Magnetically Treated Water On Some Morphological And Biochemical Properties of Mung Bean (Phaseolus Aureus Roxb.) Cuttings

اسم المؤلف: عامر راضي عبد الحسين جبار
اسم المشرف: بشير عبد الحمزة محمد العلواني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: This study was conducted in order to investigate the role of magnetically water in the rooting of fresh and aged (phaseolus aureus roxb.) cuttings, and the impact of treated water in some morphological criteria and biochemical that interfere in the induction of rooting in cuttings and fall clearly when delayed induced auxin treatment in aged cuttings. Study was conducted in the laboratories of the Department of biology at the University of Babylon, for the period 2012 - 2014 was the use of magnetic therapy devices (Magnitron) of water with three gauss 1000, 2000.3000 so as to provide a magnetic flow to distilled water and river water and used as well as the complete randomized design and analyzed the results statistically significant and valuable knowledge LSD to extract the significantly differences between the treatments and the results were as follows : Physiological parts1. Increase the number of roots significantly in cuttings when treat for 24 h.with auxin dissolved with distilled water processor in 1000 gauss amounted to 77.75 root / cutting.2. The results showed that the roots rate increased significantly in the treatment of fresh cuttings treated in distilled water processor 1000 gauss gave 64 root / cutting. As well as the rest treatments affect in rooting increase as given (58, 52.25) root / cutting for each of the treatment 2000.3000 gauss, respectively.3. The treatment of river water as well as magnetically influenced in increasing the number of roots significantly in three processes and the highest being in the 1000 gauss(84) root / cutting.4. The results showed that distilled water processor magnetic effect is significant in the processes that occur during ageing of the cuttings, which led to a reduction in rooting rate in aged cuttings, as it was observed that the treatment of the cuttings for 72 - hour in 1000 gauss is the best of the rest of the treatments amounted to 48 root / cutting. As the impact of river water processor magnetically significantly increase the number of roots in the aged cuttings as treatment given to 1000 gauss higher values 70 root / cuting.Biochemical parts.1. The results showed that chlorophyll content increased significantly when using the river water wizard three intensities (18.09, 17.43, 16.99) (spad) intensities of 1000, 2000.3000 gauss respectively.other each of distilled water and river water processor magnetically in 1000 gauss increase in chlorophyll content to 16.07, 18.57 (spad) respectively in aged cuttings.2. It was observed that the treatment of auxin dissolved in three intensities impacted significantly on the effectiveness of antioxidants defense system and the highest being at the treatment of the cuttings in auxin dissolved water magnetically processor in 1000 gauss, as was the effectiveness of the enzyme SOD 1.85 unit in the effectiveness of the enzyme CAT reached 15.30 unit and increased GSH content to 0.914 mg / g when compared with the control treatment.3. influenced the treatment of fresh cuttings in distilled water magnetically processor with three intensities are equally matched in the effectiveness of SOD and CAT, and the highest being at 1000 gauss magnetic intensity as it affected the effectiveness of the CAT to 31.62 units. Magnetic intensity also affected the same increase in GSH content reached significantly to 0.710 mg / g.4. The results showed the treatment of fresh cuttings river water magnetically wizard in three intensities increase in the effectiveness of the enzyme SOD and CAT, and the highest being at the treatment of 1000 gauss, amounting to 4.168, 45.9 U for both enzymes, respectively, as GSH content increased significantly, reaching 0.810 mg / g in the treatment of the fresh cuttings in 1000 gauss.5. I have not noticed any significant effect of distilled water processor magnetically as well as river water in the MDA content.6. The impact of distilled water processor 1000 gauss significantly in increasing the effectiveness of the enzyme SOD and CAT in aged cuttings, amounting to 2.88, 42.84 units respectively, as GSH content increased to 0.785 mg / g when the treatment of tresh cuttings in distilled water processor with 1000 gauss and also affected the rest of the intensities increase in GSH content in aged cuttings.7. increased the effectiveness of enzyme SOD and CAT when treated of aged cuttings in river water processor magnetically 1000 gauss, amounting to 2.9, 51.2 units respectively, as GSHwas significant increase in content to 0.854 mg / g when treatment aged cuttings in river water processor 1000 gauss and also affected the rest of the intensities increase in GSH content.8. The use of treated water magnetically 1000 gauss significantly effect in reducing the MDA content in aged cuttings 0.293 mg/g fresh weight.

العلاقة بين متلازمة تكيس المبايض وهرمون المضاد لمولر وبرامج تحريض الاباضة عند اجراء عملية التمنية داخل الرحم

اسم المؤلف: عبير ناظم جاسم
اسم المشرف: محمد باقر محمد رشاد فخر الدين | صباح مهدي حسين
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دبلوم عالي
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تعد متلازمة المبيض متعدد الا كياس هي من اكثر امراض الغدد الصماء شيوعا عند النساء حيث يصاب من النساء في سن الانجاب مايقارب (5 - 10%). الاعراض الرئيسيه لمرض متلازمة المبيض متعدد الاكياس هو اضطراب الدوره الشهريه مع فشل لاباضه وظهور اعراض زيادة الهرمون الذكر

التحري الجزيئي عن بعض التغيرات في الدنا المايتوكونديري للنطف لمرضى يعانون من وهن حركة النطف == Molecular Screening of Some Changes In Sperm Mitochondrial DNA In Asthenozoospermic Patients

اسم المؤلف: عدي عدنان مهدي
اسم المشرف: اسماعيل عبد الرضا عبد الحسن
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: من خلال البحوث والدراسات التي اجريت على اسباب وهن حركة النطف لدى الرجال الا انه هناك عدد من المسببات لم تحدد والى الوقت الحاضر. الا انه بالرغم من ذلك بعض الدراسات تشيربان العامل الوراثي يلعب دور في ذلك والمتمثل بعضيات المايتوكوندريا والحاوية على الدنا الم | Through researches and studies done on the causes of the asthenozoospermia in men, although there was no specific reason so far responsible for the infertility in people, but some of the studies were indicated that the genetic factors plays a role in the sperm dysmotility through the mitochondria that contained mitochondrial DNA responsible for providing the energy required for the sperm motility by production of ATP, which serve as the fuel that is consumed by sperm during motility. This study is conducted to determine the changes that could occur in the sperm mtDNA which included (common deletion, mtDNA copy number per cell and mutations in the ATPase8 and ATPase6 genes).This study was consisted of two parts as follow : The first part was consisted of two steps : The first step conducted on 71 samples from subjects suffering from the weakness of the movement of sperm and 12 samples from subjects who have a normal movement of sperm, total samples were 83 samples were divided patient samples into 5 groups depending on the percentage of the sperm motility under the classification adopted by the World Health Organization as well as a control group, that it has become 6 groups, these samples were subjected for investigation about the mitochondrial DNA deletions and mitochondrial DNA copy number by using real time PCR.On the other hand was taking 66 sample dispersed sample 56 patients and 10 healthy samples were subjected to the tests of ATPase6 and ATPase8 genes sequences for detection about mtDNA mutation.The second step was included the use of discontinuous gradient centrifugation (percoll) , which are 40 % and 80 % which represent both progressive and non progressive motile sperms, bringing the total samples 166 samples that collected and fractionated in a hospital, Kamal Al - Samarrai to treat infertility and IVF in Baghdad and examined for further molecular investigations for the period from February 2012 to October 2013.The second part was included the molecular tests that carried out after DNA extraction from the cells and this part include three steps : The first step was to investigate the common deletion in mtDNA where the results showed that the total number of deleted samples in the group (0 - 5) in the nonprogressive sperm was 81.25 %, which ranged between 6.8 - 74.7% while the ratio between the other groups 6 - 10, 11 - 15 , 16 - 20, 21 - 25 where were 75, 35.72, 28.5 , 36.3, and 12% for control group respectively. The second step was to investigate the sperm mtDNA copy number. The same groups where the results showed that the content of DNA in subjects with impaired movement of sperm was high compared with the control group where scored in some samples the highest level of gene expression 246.9 copies per cell in the group (0 - 5%) either other groups have shown lower levels were observed in groups (6 - 10) , (11 - 15) , (16 - 20) and (21 - 25) were 203.1, 180.7, 133.5 and 128.3 copies per cell, either the highest level of DNA content of the control group was 94.7 copies per cell, there was also a significant difference (P>0.05) between the normospermic motile isolated from 80% class and poor sperm movement isolated from 40 % class. The third step was included the detection of mutations by analyzing the sequences of the MT - ATAase8 and MT - ATPase6 genes which documented 59 mutations that were 23 missense and 36 silent mutations where all of them were heteroplasmic mutation except for a single mutation of the type missense (A8860G) which was homoplasmic mutation was noticed in all mtDNA copies of patients and control subjects also in progressive and non progressive sperm cells. Thought this current study novel nucleotides changes in MT - ATAase8 and MT - ATPase6 genes among groups where 3 novel missense mutations in MT - ATAase8 gene at positions (8378, 8483 and 8558), the changes of nucleotides bases were A>G, A>C, A>C and A>C respectively, replacing Asparagine to Aspartic acid, Leucine to Proline and Proline to Alanine respectively. Also two novel missense mutations were observed in MT - ATPase6 gene at positions nt (8822 and 9055) where the changes nucleotides were (C >T and G>A) that replaced amino acids (Serine to Phenylalanine and Alanine to Threonine) respectively. Silent novel heteroplasmic change as a transition substitutions in ATPase8 gene at position nt (8371) by replacing C>T without any changing in amino acid of protein. Results of this present study showed novel heteroplasmic silent mutations in ATPase6 gene at positions nt (8899, 9048 and 9060) nucleotides changes (C>T, T>C and C>A) respectively, without changing in amino acid of protein which were observed in infertile group

تقييم بعض الدلائل الحيوية وصورة الدهون وعلاقتها مع مرضى السكري من النوع الثاني == Assessment of Some Biomarkers And Lipid Profile In Relation With Diabetic Patients Type 2

اسم المؤلف: عذراء باقر حسن الشيباوي
اسم المشرف: ارشد نوري غني الدجيلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: لازال البحث مستمرعن ايجاد متغيرات جديدة قد تساعد في تشخيص ومتابعة مرض السكر وهو من المجالات المهمة في ميادين البحث العلمي حيث ركزت بعض الدراسات على حالة الانسولين بينمراكزت بحوث اخرى على صورة الدهون والشدة التاكسدية كاسباب للسكري. اجريت هذه الدراسة لمرض | The search of a new parameters for monitoring and even prediction of diabetes mellitus (DM) are still an important issue in many research fields. Some studies focused on the role of insulin status, while others concentrated on lipid disturbances or even oxidative stress disorders in the diabetes. This study was conducted on randomly selected 68 type 2 diabetic patients (27 Males and 41 Females) attending the diabetes mellitus center in Al - Sadder Teaching Hospital in Al - Najaf province, Iraq and a group of 20 apparently healthy subjects (10 Males and 10 Females) were included as a control group. The study was carried out from February 2013 to July 2013. The age of patients and control groups were range of 35 - 65years. The concentration of fasting blood glucose, cholesterol, triglyceride, LDL, VLDL, HDL, Apelin, Omentin, Vaspin, Visfatin and BMI were estimated in patients and control groups. The results show significant increase (P?0.05) in fasting blood glucose, cholesterol, triglyceride, LDL, VLDL, Apelin, Vaspin and Visfatin levels in patients compared with control groups, also the results show significant decrease (P?0.05) in HDL and Omentin level in patients compared with control groups. The results revealed that all biomarkers (Apelin, Omentin, Vaspin and Visfatin) not significant difference (P>0.05) in patients at different ages. The results also revealed that biomarkers (Apelin, Vaspin and Visfatin) levels increase significantly (P?0.05) in males than females in both patients and control groups, while the result of omentin level reveal no significant difference (P>0.05) between males and females in both patients and control groups. The results also revealed that significant increase (P?0.05) in BMI in patients compared with control groups. The results also show that biomarkers (Apelin, Vaspin and Visfatin) concentration increase significantly (P?0.05) with increasing BMI in males than females compared with control groups, while the result of omentin concentration show no significant difference (P>0.05) in patients and control groups and show lower significantly increase (P?0.05) in females than males compared with control groups. The results have been shown significant positive correlation (P?0.05) between biomarkers (Apelin, Vaspin and Visfatin) and FBG, cholesterol, triglyceride, LDL and VLDL in patients (males and females), while the results have been shown significant negative correlation (P?0.05) between biomarkers (Apelin, Vaspin and Visfatin) and HDL in patients (males and females). The results also have been shown significant negative correlation (P?0.05) between omentin and FBG, cholesterol, triglycerides, LDL and VLDL in patients (males and females), while the results have been shown significant positive correlation (P?0.05) between omentin and HDL in patients (males and females). The results also have been shown no significant correlation (P>0.05) between Vaspin and FBG in patients (males and females). The present study concluded that Apelin, Omentin, Vaspin and Visfatin levels maybe that could be adopted as markers for detection and diagnosis of diabetic patients type 2.

تقييم قابلية بعض انزيمات السليليز الفطري على تخمير تخمير بقايا النباتات لانتاج الايثانول == Evaluation of Capability of Some Fungal Cellulase In Fermentation of Plant Residues For Ethanol Production

اسم المؤلف: عذراء حرجان محسن الدحيدحاوي
اسم المشرف: فاطمة عبد الحسين التميمي | محسن هاشم رسن
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: هدفت الدراسة الحالية الى تقييم قابلية بعض انزيمات السليليز الفطري على تخمير بقايا النباتات على انتاج الايثانول من بعض الفطريات المحلية المعزولة من 50 نموذج من التربة والذي تضمن Aspergillus oryzae , A. niger, A. terreus , A.flavus , A.fumigatus, A.parasiti | The present study was conducted to proceed a Bioethanol production using some of the waste fermentation plant by cellulosic hydrolysis enzyme produced by some local fungi isolated from 50 samples from soil which were including Aspergillus oryzae, A.niger, A.terreus , A.flavus , A.fumigatus, A.parasiticus, A.nidulans, Penicilliuum chrysogenum, Trichoderma longi, Rhizopus stolonifer, Cladosporium spp, Mucor indicus, Trichothecium spp. The prominent isolates were A. oryzae, A.niger, A. terreus which were chosen for further studies and screening to producing cellulase enzymes the fungal isolates revealed variation values of clear zone It’s (5.1, 5, 5, 4.8) cm to A. oryzae, A.niger, A. terreus , A.flavus respectively. The results revealed that the suitable carbon source to the cellulase activity was induced in different raw plant substrate, the highest was produced when using Corn cobs, Rice husk and Reed reached to (1.72, 2, 1.26)IU/ ml respectively when using A. oryzae and (1.24, 1.17, 1.89) IU/ ml when using A.niger, and (1.31, 1.19, 1.93)IU/ ml when using A. terreus. but using the cellulose powder and CMC, Avical sole carbon source total cellulase given least activity compare with raw plant substrate.on the other hand, using Mandels - Weber medium activity of enzyme production by A. oryzae, A.niger, A. terreus recorded (6.51, 4.14, 5.61) U/ ml respectively when using Corn cobs and (5.79, 4.03, 5.24) U/ ml and (5.55, 3.97, 5.19) U/ ml when using Rice husk and Reed by different fungi. The results refers that , the best chemical pretreatment results were appear when using NaOH at 100Co in 30min on Corn cobs, the total cellulase activity were (1.63, 1, 1.21) U / ml when using fungal isolated to degradation after treatment, while using hot water at100Co without NaOH in 15 min to pretreatment Rice husk activity were (2.16, 2.07, 2.12) U / ml but Reed are appear high activity when pretreatment with NaOH at 100C0 in 15min activity It’s (2.28, 1.65, 2.09) U / ml. The results revealed that, The optimized conditions of the enzymes were different in this study that give activities of Fpase, CMCase and ? - glucosidases to fungus A. oryzae compared to the rest of the fungi using Corn cobs as carbon source, it reached to(2.21, 2.30, 46.72) IU / ml respectively when pH6, and temperature tested for these enzymes recorded (1.64, 1.61, 30.86) IU\ ml respectively at a temperature of 30 C° and when using the concentration of substrate gave activity (2.4o, 2.48, 42.34) IU / ml of 6 % from Corn cobs and recorded when using nitrogen source concentration at 6 % (1.92, 1.89) IU\ ml in KNO3 and (NH4)2SO4 to Fpase and CMCase and the effectiveness of ? - glucosidase at the same nitrogen source (43.54, 50) IU / ml. on the other hand, showed enzymes Fpase, CMCase and ? - glucosidase high activities to ? - glucosidase, CMCase to fungus A. oryzae compared to the rest of the fungi using Reed as carbon source reached (2.31, 2.21, 46.15) IU/ml respectively at pH 6, and at different temperatures were (1.66, 30.94) IU/ml to Fpase and ? - glucosidase at 30 C°, but CMCase gave the highest efficacy (1.60) IU \ml at 25 C° and decreased effectiveness to (2.60, 2.57, 49.69) IU / ml at 6 % of the carbon source recorded effectiveness of the enzyme Fpase (1.19, 1.09) IU / ml when using (NH4)2PO4 and (NH4)2SO4 at 6 % as a nitrogen source of from either enzymatically CMCase and ? - glucosidase was effective at same nitrogen source (1.29, 2) and (35.19, 40.41) IU / ml respectively.The results showed when you use a Rice husk recorded higher effective enzymatic Fpase and CMCase and ? - glucosidases of fungus A. oryzae was (2.32, 39.27) IU /ml for Fpase and ? - glucosidases at pH 6 but CMCase gave the highest efficacy at 5 pH as recorded (2.04) IU / ml at temperatures 30 C°, reached to (1.64, 1.58, 29.13) IU / ml respectively for Fpase and CMCase and ? - glucosidases at a temperature of 30 C°, and when using 6% of the substrate was effective (2.47, 2.19, 44.86) IU / ml, activity recorded when using (NH4)2PO4 and (NH4)2SO4 at 6 % as a nitrogen source of (1.1) IU / ml to FPase on either enzymatically CMCase and ? - glucosidase was effective when use same a nitrogen source (1.18, 1.83) and (31.92, 36.91) IU/ml, respectively. In addition, The results refers when precipitation by ammonium sulfate specific activity were (38.69, 1.25, 5767.5) IU of the enzyme with 0.012 mg protein and then, Elution protein of crude enzyme solution of A.oryzae from DEAE - cellulose column was shown to the protein was separated in (33) fractions.It was found that only the fractions (F - 8) contained cellulase activity, while fraction (F - 30) eluted in column containing buffer and different concentrations of NaCl then using gel filtration and gave three peak of enzymes alone and Characterize enzymes purified FPase activity was found at 5.5 pH which (0.91) U and temperature was having activity at 25 and 30 C°, were (0.14, 0.15) U and CMCase found that the enzyme exhibited maximum activity at 25 C° was (0.33) U but 30 C° for ? - glucosidase was (38.22) U and the molecular weight of the protein was found to be about (38, 52 and 49) kD for (FPase, CMCase, ? - glucosidase) respectively. Finally, estimated ethanol density (0.80 - 0.91) g /ml and ethanol concentration (60 - 97) % but using chromic acid test of purified ethanol the complex enzyme with Reed, complex enzyme with Corn cobs recoded high value of ethanol which were (0.98, 1.72, 1.87, 1.89 ) % and (1.09, 1.83, 1.92, 1.99) % respectively but complex enzyme with Rice husk were given (1.01, 1.12, 1.48, 1.79) % in 4, 5, 6, and 7 days respectively on the other hand, when using the A.oryzae and A.terreus with Reed gave high ethanol value from other fungi with substrate in this study, and observed Rice husk in all results was recorded less absorbance at 350 nm and determination of ethanol concentration by gas chromatography mass used ethanol concentration 99 % as standard, also we showed the ethanol when using enzyme pure with substrate in which were (93.7, 97.1, 88) % ethanol to enzyme with Corn cobs and Reed and Rice husk respectively

تشخيص بكتريا Neisseria gonorrhoeae بالطرق التقليدية والجزيئية في المرضى الذكور ودراسة مدى تاثيرها في حدوث الحذوف في موقع AZF == Conventional And Molecular Diagnosis of Neisseria Gonorrhoeae In Male Patients And Study Its Suspected Effects In Microdeletions In Azf Locus

اسم المؤلف: غزوان علي مسلم الرماحي
اسم المشرف: عبد الرضا عبد الحسن اللامي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study includes 82 clinical samples from male patients suspected to have gonorrhea (urethral discharge with dysuria). Two types of samples were collected from each patient, urethra swab and blood samples from patients who attended Al - Yarmouk teaching hospital in Baghdad city, Clinics and private laboratories during a period from December 2012 to April 2013. All of them were married and have children's, and 20 samples were taken from apparently healthy subjects.Cases caused by gonococci were diagnosed by finding the Neisseria gonorrhoeae bacteria in the samples using microscopic examination and culturing on rich media chocolate agare for growth bacteria and selective media modified Thayer martin agar (MTM) contact antibiotic to prevent growth of all the types of bacteria except N. gonorrhoeae.Microscopic examination using specific kit for gram stain, showed gram negative diplococcus, like bean shape. Culturing on rich media revealed that 82 (100%) samples positive but re culturing on Modified Thayer martin media revealed that 76 (92.68%) out of 82 positive. Then biochemical test had the same results.Results of the molecular diagnosis of the bacteria causing gonorrhea using specific primers that were specific for the Orf1 gene, revealed that 80.26% of samples (61 out of 76) were positive. Results of the microdeletion in Y chromosome AZFc region revealed that no microdeletion were occurred in SY - 254 STS and BPY - 2 gene.This study, provided high specificity and sensitivity for the diagnosis of gonorrhea using PCR technique which is cheaper and faster than traditional methods. Also, PCR - based method for detection of N.gonorrhoeae can be readily used in hospitals and laboratories.

تقييم وعزل فيروس الانفلونزا للانسان ودراسة الاستجابة المناعية في الجرذان التجريبية == Evaluation And Isolation of Human Influenza Virus And Studying Their Immune Response In Experimental Rats

اسم المؤلف: فاديه مهدي مسلم العبيدي
اسم المشرف: عبد المجيد عبد الله السعدي | يونس عبد الرضا الخفاجي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: This study has been conducted for the first time in Najaf / Iraq, and the study included the preparation of a local inactivated vaccine (whole and subunit).The number of cases infected with seasonal influenza virus were 647 cases out of one thousand suspected case. They were distributed into eleven groups. Seasonal influenza virus was detected by two diagnostic methods (rapid test device and real time PCR).The present study has reflected that the most diagnosed cases infected with seasonal influenza virus during the period extended from March 26, 2012 up to April 30, 2013 represented by were type A (H3N2) (283) cases. Whereas H1N1 (148) cases, H3N2+H1N1, (56) cases, H1N1 + H3N2 + B were (30) cases and 130 cases were infected with type B.Current study also revealed that age group of 46 - 60 years old infected with seasonal influenza virus were (159) case including (368) male and females were (279) of different infected group..The spread of seasonal influenza virus in Najaf varies from one region to another depending on the population density. Clinical cases were collected randomly during the months of the year. It has been found that the spread of disease has something to do with temperature and humidity as high humidity and low temperature lead to increased spread of the disease because of their relationship at the stage of the spread of epidemic influenza that spread from Dec 26, 2012 and up to April 1, 2013 in addition to expatriates infected..Two techniques were used for isolation of seasonal influenza virus first inoculation of embryonated chicken egg and secondly chicken fibroblast cell culture.It was found that at the embryonated egg lethal dosage (ELD 50 / 0.5 ml) (109.6) whereas tissue culture infective dosage (TCID50 / 0.1 ml) was (109.3) of (H1N1+ H3N2) and type (B) virus..Virus was purified by two ways that are depending on the vaccine type. The gamma irradiation that dosage used for inactivating vaccine of the purified virus which was extracted from allantoic fluid and tissue culture was (5.702) mSv/h for subunit virus vaccine type whereas gamma irradiation dosage for complete (whole) virus vaccine was (8.816) mSv/h. The results proved that immunoglobulins (IgM, IgA and IgG) levels as well as (IL - 17, IL - 10 and INF - 1?) indicated significant differences among vaccinated, infected and control groups.Subunit and whole virus vaccine extracted from chicken fibroblast cell culture are considered the best of derived from virus propagated in embryonated chicken eggs.Subunit virus vaccine is better than the whole virus vaccine derived from virus propagated in embryonated chicken eggs and extracted from chicken fibroblast cell culture.The current study also reflected that locally prepared virus subunit vaccine and purified whole virus killed vaccine capable of inducing humoral and cellular immune response in experimentally vaccinated rats..Histopathological changes appeared clearly on tissue sections (lung, trachea, heart, liver, spleen, kidney) of the infected group as compared with control and the vaccinated groups.

دراسة بعض المؤشرات المناعية لدى بعض المرضى المصابين بفيروس المليساء المعدية في محافظة ديالى == A Study of Some Immunological Parameters In Some Patients With Molluscum Contagiosum In Diyala Province

اسم المؤلف: رغد ياسين اعويد
اسم المشرف: عباس عبود فرحان الدليمي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: المليساء المعدية مرض يسببه فيروس (MCV) الذي ينتمي لعائلة POXVIRUS.المليساء المعدية حيث انه مرض غير قاتل وشائع في جميع انحاء العالم, قد يشترك مع اورام المليساء التهابات قليله, المليساء المعدية يستمر لشهور او سنوات..اجريت الدراسة الحالية للفترة من 1 تشرين ا | The molluscum contagiosum (MC) virus (MCV) is a dermatotropic poxvirus, and the causative agent of MC. MC is nonlethal, common andworldwide. Additionally, little inflammation is associated with MCpapules, and MC can persist for months to years. The present study was conducted for the period from1 November 2013 to 30April, of 2014 in outpatient clinic of Baquba Teaching Hospital in city. The study aimsat assessing the immune status of patients infected with molluscumcontagiosum through the measurement ofthelevel of immunoglobulin(IgG, IgM)and the level of complementcomponents especially (C3, C4) by radial immune diffusion assay and measurement of the level of interleukin 18 R1 by Elisa assay which immune responses are key for the eventual resolution of MC. 75 patients were diagnosed with clinical lesions of MCV on different areas of the body, age of patients ranged from(2 - 50 years) including 40(53.3%) males and 35(46.7%) females.The control were 26.6±15.4 years (range from 2 - 50 years), 8 (53.3%) of them were males and 7(46.7%) females.After theexamination by single radial immune diffusion and Elisa kit.The results showed the patientswere equal or less than 16 years, 24 (32%) from 17 - 30 years, 19 (25.3%) from 31 - 45 years and 12 (16%) above 45 years and 40(53.3%) males and 35(46.7%) females, no static significant difference showed between the MCV infection and either the sex or age.The results revealed the level of IL 18R1in patient the mean±SD were 677.15±874.22 ng/ml while in control were 178.46±31.79 ng/ml. There was significant statistical difference between both groups as patients with MC had high level of IL18R1 than control.Themean±SD of IgMinpatients were 1946.6±825.6 mg/dlwhile in control were 140.1±68.7mg/dl This result was highly significant which indicates that patients with MC had higher level of IgM than control In contrast, patients had lower levels of IgG than control, The mean±SD of IgG in patientwere 221.9±96.7mg/dl while in control were 1229.9±299.7mg/dl. This result was highly significant. Also the patients had lower level of C3 and C4 than control. The mean±SD of C3 in patients were 109.6±64.8mg/dl while in control were 120.8±22.1mg/dl. The mean±SD of C4 in patients were 27.8±12.7mg/dl while in control were 38.7±9.8mg/dl. These results were statistically significant. Eleven 9 (14.7%) of patients had positive CRP while 12 (80%) of control had positive CRP which was statistically highly significant. Forty seven (62.7%) patients were from rural area while 28 (37.7%) from urban. There is no statistical difference between both groups as. Family history of MC and Recurrence of MC in patients were nostatically significant difference to infection by MCV.

دراسة امكانية الاستفادة من تركيز بروتين المصل الفعال نوع سي بروتين التفاعلي CRP في التشخيص المبكر للاصابات البكتيرية لحديثي الولادة وتحديد توقف اعطاء العلاج من خلاله == The Study of Usefulness of C - Reactive Protein Concentration In The Early Diagnosis And Determining The Duration of Antibiotic Therapy of Suspected Neonatal Bacterial Infection

اسم المؤلف: قاسم ابراهيم حسين
اسم المشرف: ايمان ناطق ناجي البياتي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ان التشخيص السريع لاصابات تجرثم الدم في الاطفال حديثي الولادة من الامور المهمة وذلك لخطورة الاصابة المؤدية الى الموت.الغرض من دراستنا هو امكانية استخدام فحص سي - البروتين الفعال في التشخيص المبكرلهذا المرض البكتيري وكذلك استخدامه في تحديد نقطة التوقف عن ا | Rapid diagnosis of bacterial infections of newborn infants is very important itcause of morbidity and mortality. Aim of the studyThe aim of the studyis to examin the possibility of using C - reactive protein in the early diagnosis of neonatal sepsis, as well as its use in determining thestop point of the antibiotic treatment.Patent, Materials and methods This study was conducted at the Hospital of Ibn - Albaladi for children and women in Rusafathe Healthoffice / Baghdad.Iraq from February 2013 to February 2014.The number of patients with bacterial infection was 102 (62%) of the total number of patients (165) who were admitted to the neonatal care unit aged less than 28 days and their weights was more than 1500 g,. The number of males was 92 (56%) and the number of females was 73 (44%), the number of patient withearly onset sepsis EOS (less than three days) was 43 (42%) and with late onset sepsis LOS for (more than three days) was 59 (58%), 43 (57%) ofEOSwas normal deliveries infected and cesarean deliveries infected 33 (43%), while51 (57%) of LOS was normal deliveries infected and cesarean deliveries in LOS was 38 (43%). Blood samples were collected and divided into three portion : bloodculture, CRP andhematological profile.1 - Blood culture.Blood was collection from neonatal sepsis patientinto special blood culture bottle and incubate in bact /Alert 3 D device when growth of bacteria then subcultured on blood agar, MaCconkey agar, and incubatedusingvitek /2 to diagnose bacterial infection anddeterminetheir susceptibility to antibiotic treatment.ResultThe Compact results indicated that 63 (38%)of patient were negative and 102 (62%) were positive out of them 23 patients (22.6%)were infected with gram negative bacteria including {Klebsiella pneumonia 30%, Acinetobacterlowffi 17%, Enterobacter cloaca 13%, } while 79 (77.4%) were infected with gram positive bacteria including { staph Homins and staph hemolyticus each 28% then staph epidermidis 13% and staph aureus 10%, while strep spp was 6% }. 2 - c - reactive protein.It was done by twomethod for the same samples in order to characterize the accuracy of the diagnosis. The first method was agglutination test (method 1) the cut off value for positive result ? 6 µg % 79 (48%)were negative, while 86 (52%) werepositive, while the second method was immune test (method 2) µµµusing Nycocard device werethe cut off value for positive result ? 5 µg % the results 102 (62%) were positive while 63 (38% negative.3 - hematological profile.The hematological profile of our patient included PCV, WBC, neutrophil, lymphocyte, monocyte, count were measured using Emerald automated Hematology device. The results of these tests werenot compatible with the results of the blood culture, but normal result were compatible with negative blood cultures. The results adopted of blood culture confirm the presence of bacterial infection and then compared with theresults CRP test and blood profilefor the same samples.Negative result of blood culture and CRP test were 54 (33%) while positive results of blood culture and CRP test were recorded and improved after taking treatment for (, 3, 5, 7,) days for (40, 31, 5)patients respectively.ConclusionWeconcluded C - reactive protein test was useful in the diagnosis of bacterial blood infections and determine the stop - point otreatment. Gram negative bacterial isolates were sensitive impinim, livofloxacin and Amikacin while they were resistant to Ampicillin, Ampicillin /Sollbectam and Gentamycin. on the other hand gram positive bacteria were sensitive to Amikacin, Ampicillin/sulbactam Ciprphloxacin, and Tigecyclin and were resistant to Erythromicin, and Oxacillinand Benzylpencillin.

دراسة كفاءة بعض المضادات الحيوية ذات المناشئ المختلفة على بعض انواع البكتريا المعزولة من حالات مرضية مختلفة == The Efficacy Study of Some Antibiotics From Different Origins On Some Pathogenic Bacteria Isolated From Different Cases

اسم المؤلف: ليلى عاصي خزعل
اسم المشرف: نجدت بهجت مهدي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: كركوك
الصفحات الاولى:
المستخلص: شملت الدراسة جمع 278عينة سريرية تضمنت نماذج من اخماج المجاري البولية ومسحات الجروح والحروق وعينات الدم ومسحات الاذن الوسطى من مستشفى كركوك العام ومستشفى طوز العام وللمدة من ( 2013 - 4 - 15) ولغاية ( (2013 - 10 - 15. اظهرت نتائج الزرع البكتريولوجي الاولي | The study included the collection (278) clinical samples included the same of urinary tract infections and swabs of wounds and burns, and blood samples and swabs from ear from Kirkuk General Hospital and Tuz General hospital in period from (2013 - 4 - 15) until (2013 - 10 - 15). The results of the first Bacteriological implant using the blood Agar and Macconkey agar emergence of planting positive in (148) of the total sample (278) sample rate (53.33%). Diagnosed colonies developing depending on planting characters and biomicroscopy and tests biochemical as possible diagnosis (90) the isolation of clinical divided to (30) isolation of each of the bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa) divided to (28) isolates of Urine and 21 isolated from wounds and 25 isolates of burns and 11 isolated from blood and 5 isolates from the ear of the total isolates, confirmed diagnosis using the API Staph System and API 20E system. The study included examination of sensitivity using discs toward the 15 antibiotic for three types. The isolates showed to different in resistant rate, and was Anti Chloramphenicol is the most effect on the bacteria S.aureus where all isolates were sensitive to him 100%, and was an anti Imipinem, Nitrofuranation the most effect on bacteria E.coli where the percentage of sensitivity to two anti - (90%), either Anti Amikacin was the most impact on the isolates of Ps. aeruginosa where all isolates were sensitive to him ratio (100%) I tested the sensitivity of bacterial species toward (5) antibiotic (Amoxicillin Augmantine, Cefotaxime, Ciprofloxacin, Tetracyclin) concentrations of certain several different origins for each antibiotic using the method of casting dishes, use an anti Amoxicillin concentration (25) mg / ml and origins different (Iraqi, Indian, Turkey, United Arab Emirates) The results show that the origin United Arab Emirates is the most efficient than the rest of other. Use the adversaries (Cefotaxime, Augmantine) concentration (10) mg / ml and three - origins (Turkish, Indian, United Arab Emirates), oukd show that Origin United Arab Emirates is the most efficient of the rest of the origins Other. also used anti Tetracyclin concentration (10) mg / ml for three origin (Iraqi, Indian, Chinese). show that Origin Iraqi is the most efficient of the rest of the origins Other.and used anti Ciprofloxacin concentration (5) mg / ml for four origin (Iraqi, Indian, Turkish, United Arab Emirates) and show that the United Arab Emirates and Turkish origin is more efficient than the rest of other origins.Bacterial isolates showed multiple drug resistance to antibiotics where all isolates Ps. aeruginosa with resistance to multiple antibiotics by (100%) and showed isolates of S.aureus resistant multi - rate (93.3 %) and showed isolates of E.coli (96.6%) as well characterized bacterial isolates in multiple resistance to antibiotics Alpittalaktam particularly where the rates of multiple resistance (93.3 %) of the isolates of E.coli and (100%) isolates Ps.aeruginosa and (93.33%) of the isolates by S.aureus.the rapid Iodine standard method was used for screening for susceptibility of bacterial isolates to produce enzymes Beta - Lactamase have shown (73) the isolation of a positive result from the total (90) isolation rate (81.1%), were distributed among the isolates produced (27) isolated from bacteria by S.aureus (90 %) and (25) isolates of E.coli bacteria by (83.3%) and (21) isolated from bacteria Ps. aeruginosa (70%).to detect broad - spectrum Beta - Lactamase enzymes is used discs method adjacent and the results showed that there are (24) isolation of the total (73) is positive for examination by (32.87%) distributed on (12) isolated from bacteria S.aureus by (44.44%) and (5) isolates of E.coli bacteria (20%) and (7) of the bacterial isolates Ps. aeruginosa by (33.33%).Tested susceptibility bacterial isolates to produce mineral Beta - Lactamase enzymes Metallo ? - Lactamase using IMP - EDTA Combination disc and three isolates can from the production of the enzyme (4.10%) by two isolates of bacteria Ps.aeruginosa by (9.52%) and one isolate of the bacterium E.coli (4%).

تحديد شدة الاصابة الحادة والمزمنة لداء المقوسات الكوندية بواسطة مستويات (IL - 6, IL - 8, TNF - ?) == Determination of Acute And Chronic Toxoplasmosis By Estimation of IL - 6, IL - 8 And TNF - ? Levels

اسم المؤلف: لينا قاسم كاظم الزهيري
اسم المشرف: بان نوري القاضي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: المقوسات الكونديه من الطفيليات الشديده النجاح في انشاء اصابة مزمنه طويلة الامد. وقد اجريت الدراسة الحاليه على 366 امراة (117 مجهضات، 141 حوامل، 108 غير متزوجات) جمعت من مستشفيات مختلفة في بغداد. جمعت عينات الدم لجميع المجاميع المدروسة خلال الفترة مابين تش | Toxoplasma is a highly invasive parasite which establishes a life - long chronic infection. The present study was performed on 366 women (117 miscarriages, 141 pregnant and 108 singles) aged (17 - 35) years old from different hospital in Baghdad. Blood samples were collected from all patients during the period of Oct.2013 until of Jan. 2014. Anti - toxoplasma antibodies in the sera of all samples by using two tests, Latex test (LAT) and ELISA (IgG), (IgM) while, the proinflammatory cytokine were estimated by evaluation of the cytokine such as (IL - 6, IL - 8, TNF - ?). The results were showed that : ? The infection rate of toxoplasmosis by LAT was (41.53%) which was more sensitive than ELISA IgG (36.85%), and miscarried women were diagnosed by higher anti - toxoplasma IgG antibodies 62/117(52.99%), while pregnant result were lower 30/108 (27.78%). ? The most frequent age group of chronic infection with T.gondii was (23 - 28) year in both miscarriage and pregnant women, and represented by 45.16%, 40% respectively. While the most frequent age group for a single woman was (17 - 22) years, and represent 46.511% of the total number of this group.? Seroprevalence of toxoplasmosis by ELISA IgM test revealed low sensitivity in different studied groups 25/366(6.830%), and miscarried women were diagnosed by a highly significant (P ?0.01) percentage of acute toxoplasmosis14/117(11.965%).and The age group of (17 - 22) years old diagnosed high significant (p?0.01) percentage of acute toxoplasmosis in all studied groups.? According to the distribution of infected miscarried women with toxoplasmosis and gestational age by ELISA IgM the result showed that most of miscarriages were occurred at the first trimester which considered high significant (p? 0.01) 8/14(57.42%). While, chronic infected women at the second trimester (4 - 7 month) diagnosed by significantly (P?0.01) higher percentage of miscarried 24/62(38.71%).? The level of pro - infalmmatory cytokine (IL - 6) in acute infected miscarried women with toxoplasmosis distincted with a significantly high increase (P? 0.05) (177.31±12.53 Pg/ml) in comparison to chronic infected one (119.36 ±14.07 Pg/ml).While, chronic infected pregnant women was low significantly (P ?0.01) (48.41± 2.79 Pg/ml) in comparison to acute infected one and other studied groups, ? The acute infected singles was (118.98± 15.68 Pg/ml) higher non - significantly (P?0.05) than chronic infected one (112.65 ± 17.91 Pg/ml) and both disease activity were significantly higher than its level in healthy singles.? The level of TNF - ? in acute infected miscarried women distincted with significantly higher (P?0.05) (97.46 ± 5.37 Pg/ml) in comparison to chronic infected women (69.55 ± 4.58 Pg/ml), Whereas the mean level of TNF - ? in pregnant infected women during acute phase (62.31 ± 3.82 Pg/ml) was significantly (P?0.05) higher than chronic infection (21.42 ±2.74 Pg/ml)? Single infected women showed high significant increase (P?0.05) of TNF - ? level (50.48 ± 7.42 Pg/ml) in comparison to chronic infected one (35.46± 5.36 Pg/ml) and both disease activity scored high significant increase (P?0.05) of TNF - ? in comparison to healthy singles (18.29 ± 1.02 Pg/ml).? Finally, the mean level of IL - 8 in miscarried women with positive infection with T. gondii during acute phase (187.92 ± 15.38 Pg/ml) was significantly (P?0.05) higher than chronic phase of infection (140.57 ± 13.87 Pg/ml).Whereas the mean level of IL - 8 in acute infected pregnant women (110.32 ± 9.13 Pg/ml) was higher significantly (P?0.05) than healthy pregnant (91.49 ± 7.59 Pg/ml) and healthy singles (88.82 ± 5.62 Pg/ml).? Infected single women showed highly significant (P?0.01) increased levels in both diseases activity in comparison to single women without infection (88.82± 5.62 Pg/ml).

دراسة بكتريولوجية لبعض الاجناس البكتيرية السالبة لصبغة غرام المقاومة لمضادات البيتالاكتام والمعزولة من اخماج المجاري البولية في محافظة ديالى == Bacteriological Study of Some Genus For Bacterial Gram - Negative Resistant To ? - Lactam, Isolated From Urinary Tract Infections In Diyala Province

اسم المؤلف: محمد خضير عباس النعيمي
اسم المشرف: عباس عبود فرحان الدليمي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: شملت الدراسة 300عينة جمعت من مرضى مصابين باخماج المجاري البولية وتم جمع العينات في مدينة بعقوبة من مستشفى البتول للولادة والاطفال ومستشفى بعقوبة التعليمي، للمدة بين 1/9/2013ولغاية1/1/2014.اظهرت نتائج الزرع البكتيري على اوساط اكار الماكونكي واكار الدم ووس | Three hundred urine from patients suffering from urinary tract infection from Baaquba Teaching Hospital and Al - Batool Hospital in Baaquba city for the period from 1/09/2013 to 1/01/2014. The results reved that 66 isolates are belonging to bacteria of Gram negative (57.4%), 25 (37.78 %) Escherichia coli, 22(33.33%) Proteus mirabilis, 9(6.06%)Klebsiella pneumonia, 5(7.57%) Enterobacter spp for 4 (25%) Enterobacter cloacae and 1(5.51%) Enterobacter aerogenes and5(7.57%) Pseudomonas aeruginosa by using diagnostic phenotypic, biochemical tests and confirm the diagnosis using regular aPI20E. Investigation of some virulence factors showed that both Escherichia coli and Proteus mirabilis produced haemolysin 52% and 90.9% respectively. The production of biofilm by local isolates was detected in Congo - red way, Isolates of Escherichia coli and Proteus mirabilis had been shown the ability to produce biofilm 92% and 90.9% respectively. The results showed that all isolates of Proteus mirabilis produced urease by 100%, while the results detect that the isolates of Escherichia coli not able to produce this enzyme. Proteus mirabilis showed ability to produce swarming with 100%. Siderophore production by Escherichia coli and Proteus mirabilis was 48%, 9% respectively. Eschrichia coli, and Proteus mirabilis produced bacteriocin with percentag of 32% and 50% respectively.The production of ? - lactamase by Eschrichia coli and Proteus mirabilis was 60% , 40.9% respectively, also the isolated had the ability to produce the Extendended spectrum ? - Lactamase enzyme by using disc Approximation, The production from each ofEschrichia coli and Proteus mirabilis 12%, 31.8 % respectively. Results of Metallo? - Lactamase by using the Imp - EDTA combination indicated that E.coli and P.mirabilis were 12% and 13.6% respectively. Isolates of E.coli showed highest resistance rate 92% for Augmentin while isolates of P.mirabilis showe higher resistant to cefotaxime 81.8%. The results showed that multiple resistance pattern for antibiotic 43 (91.5%) resistant to (2 - 5) antibiotics, Isolates of E.coli showed highest multiple resistance rate 92% while isolates of P.mirabilis rate 90.9%. The Minimal inhibitory concentration (MIC) for namely two antibiotics Cefotaxime, Ceftazidime were determined.MIC values of these antibiotics ranged between (2 - <1024), (4 - <1024) µg\ml E.coli and (2 - <1024), (8 - <1024) µg\ml P.mirabilis respectively. The results of molecular detection of ESBL genes (bla TEM and bla SHV) by using PCR technique, (9) samples from (10) total, divided into 3(100%) E.coli and 6(85.7%) P.mirabilis were harboring bla TEM gene based on the presence of 950 bp bands in 1% agarose gel. while results detect that the isolates of Escherichia coli and Proteus mirabilis not harboring bla SHV gene.

دراسة وراثية وبكتريولوجية على بكتريا تثبيت النيتروجين التكافلية Ensifer meliloti == Bacteriological And Genetic Study On Symbiotic Nitrogen Fixing Bacteria Ensifer Meliloti

اسم المؤلف: محمد زهير ناجي المرعب
اسم المشرف: حسن فاضل ناجي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: عزلت عشر عزلات من بكتريا Ensifer meliloti من العقد الجذرية لنبات الجت Medicago sativa L. بعد ان جمعت من مناطق مختلفة من محافظة بابل خلال المدة من شهر تشرين الثاني/2012 لغاية شهر شباط/2013. شخصت العزلات جزيئيا بتقنية تفاعل البلمرة السلسلي فضلا عن طرق ال | Ten isolates of Ensifer meliloti were isolated from root nodules of Medicago sativa L. collected from different areas of Babylon province during a period from November - 2012 to February - 2013. The isolates were molecular identified using polymerase chain reaction technique, in addition to traditional methods (morphological and biochemical). All isolates were harboured nodbox and mucR genes and produced cyclic ? - (1, 2) glucans, cellulose fibers and lipopolysaccharide.The ability of isolates to grow at different degrees of pH, temperatures and different concentrations of sodium chloride were tested. It was found that the optimum pH for growing of Em1, Em3, Em4, Em6 and Em8 isolates was pH7, while for the isolates Em2, Em5, Em7, Em9 and Em10 was pH8. The optimum temperature for growing of Em1, Em3, Em5, Em7, Em8 and Em9 isolates was 30?C, while the optimal growth of Em2, Em 4 and Em6 isolates was 35?C, Em10 isolate showed significantly extremism to grow at temperture reached to 45?C. The optimal percentages of NaCl for growing of Em1, Em3, Em4, Em5, Em6, Em8 and Em9 isolates were 2%, for Em7 isolate was 3% and for Em2 and Em10 isolates were 4%. The effect of different degrees of pH on symbiotic properties was studied. Em1 and Em8 isolates that grew at pH6.5 ; all isolates that grew at pH 7, pH 7.5 and pH 8 were able to infect the root hairs of alfalfa plants. The isolates that grew at pH6.5 (except Em1 and Em8 isolates) and all isolates that grew at pH8.5 were failed to infect the root hairs and did not formed nodules and resembled to the control plants (without inoculum).Different concentrations of NaCl on symbiosis was studied, it was found that all isolates that grew at 2% and Em2 and Em10 isolates that grew at 3% formed nodule, while Em1, Em3, Em4, Em5, Em6, Em7, Em8 and Em9 isolates that grew at 3% and all isolates that grew at 4%, 5% and 6% did not elicited nodules on the roots of alfalfa plant. Light microscopic observations of root hairs inoculated with Ensifer meliloti isolates showed the root hair deformations like curling, waviness, swelling and formation of shepherd`s crook and infection thread. These consequent changes lead to emerge the mature nodules having five distinct zones viz., distal meristematic, infection, inter, nitrogen - fixing and proximal senescence zones.The gel electrophoresis results of plasmid DNA of isolates showed that these isolates harboured two megaplasmids. When the isolates exposed to extreme factors like pH, temperture and NaCl, it was found that the isolates lost these plasmids and its ability to elicite the nodules and failed to fix the nitrogen. This study showed that the nitrogen fixing bacteria had essential role to support the plant growth and increase their productivity, and recommended to use the isolate Em10 as example for biofertilizers and the best alternative to the chemical fertilizers

دراسة التاثيرات السمية للـ Gliotoxin المنتج من العفن Aspergillus fumigatus باستخدام تخمرات الحالة الصلبة == Study Toxicity Effects of Gliotoxin Produced By Aspergillus Fumigatus Using Solid State Fermentation

اسم المؤلف: محمد عادل نوري
اسم المشرف: عبد الكريم جاسم هاشم
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تم اختبار قابلية عشرة عزلات للعفن Aspergillus fumigatus على انتاج الغليوتوكسين بوساطة تخمرات الحالة الصلبة وباستخدام الرز كوسط زرعي (ركيزة). اشارت نتائج الغربلة ان العزلة AF - 5 كانت هي الافضل واعطت اعلى انتاجية.كانت الظروف المثلى لانتاج السم على وسط الر | The ability of Ten Aspergillus fumigatus isolates for gliotoxin production were screened by solid state fermentation (SSF) using rice medium as substrate. The results indicated that, the AF - 5 isolate was the highest gliotoxin producer.The optimum conditions for gliotoxin production by AF - 5 isolate on rice medium were : - inoculum size 6×106 spores, moisturizing ratio (5 : 1) (w : v) with distilled water and incubation at 37 ?C for 10 days. Gliotoxin was purified from crude extract of AF - 5 isolate after production under the optimum conditions using solid state fermentation SSF. Purification of gliotoxin was achieved by two steps including : filtration and solid phase extraction. These processes were accessed to remove debris from crude extract and gives pure gliotoxin. The gliotoxin concentration was (122.6 ppm), which was detected by Thin layer Chromatography and High Performance Liquid Chromatography.The result of cytotoxicity effect of both purified extract and standard gliotoxin on human lymphocytes with exposure time of 24 hours at four different concentrations 25, 50, 100 and 200 ppb showed growth inhibition percentage 21, 39.10, 61.99, 87.45% and 17.89, 34.92, 58.34, and 85.22% respectively. The cytotoxicity effects have been done using conversion of the MTT to MTT formazan. After DNA was extracted from lymphocyte treated with toxin and analyzed by electrophoresis on a 1% agarose gel, gliotoxin appeared to have ability to damage the DNA. Results showed that both growth inhibition and DNA damage were increased gradually with the increasing of gliotoxin concentration.

دراسة انتشار انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا == Studying The Spreading of Fungus Saprolegnia SPP. In Waters of Diwaniyah City And Characterization The Molecularly

اسم المؤلف: معتز محمد عزوز الزاملي
اسم المشرف: ماجد كاظم عبود الشبلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شملت الدراسة الحالية عزل وتشخيص بعض الفطريات المائية ومنها انواع الفطر Saprolegnia spp. في مياه مدينة الديوانية وتوصيفها جزيئيا. عزلت الانواع التابعة لهذا الفطر وهما Saprolegnia parasitica وSaprolegnia ferax من الانهار والبحيرات والاسماك, وتم قياس الخصائ | The present study is concerned with isolation and identification of some aquatic fungi, including species of Saprolegnia spp. in waters of Al - Diwaniyah city and molecular characterization. Two species, Saprolegnia parasitica and Saprolegnia ferax, have been isolated from rivers, lakes and fishes. The physical and chemical environmental characteristics have been measured. The types above have been examined to extract the DNA. The (PCR) technique has been used to examine the presence of protease enzyme. The results of isolation and identification showed that the percentage of the two species were 64% in rivers areas 40% in the lakes areas and 50% in fishes. The rates represented the examined samples which were 60 samples. AS well as isolates was in rivers 16 isolation and in Lakes 10 isolates either in fish was 5 isolation and thus the total isolates 31 isolation, The S. parasitica species is more apparently found during (February, 2014), the number of samples was 12, the less presence of this species in (April, 2014), where the number of sample, was 3. The S. ferax species is more apparently presented in (February, 2014), the number of samples was 10. The less presence was in (April, 2014), the number of samples was 2. The results showed that the number of isolation of Saprolegnia spp. study area as it was S.parasitica species in were 6 in rivers, 2 in lakes, and 3 in fishes. The second species, S. ferax showed number of isolations which were 10 in rivers, 8 in lakes, and 2 in fishes. The PCR examination has used to detect the rDNA gene which responsible for proteases gene and whether it is found in the two species of fungi. The express way has been used to extract DNA and according to the instructions of manufacturer company. The concentrations of DNA in first species were 121.8 - 73.0 mcg/microliter and purity 1.87 - 1.80.In the second species the concentrations of DNA were 2360.4 - 166.9 mcg/microliter and purity 2 - 1.75. The results, by using PCR technique, showed that the percentage of protease in first species was 25% in rivers. In lakes and fish was 100%.Concerning the second species, the percentage of enzyme was 42% in rivers, 40%in lakes, and 50% in fishes. It can be concluded that there is a diversity in fungi species with respect to study areas and enzyme secretion in the stages of fungi infection. The enzyme is more apparently found in S.parasitica species compared with S.ferax species.

دراسة مصلية دمية لداء المقوسات في النساء الحوامل والاطفال حديثي الولادة في محافظة القادسية == Bloody Serological Study of Toxoplasmosis In Pregnant Women And Newborns In Diwaniyah Province

اسم المؤلف: مروة جبار مزهر العبادي
اسم المشرف: خيري عبد الله داود العكيلي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية على الاطفال حديثي الولادة في محافظة الديوانية للمدة من بداية شهر تشرين الثاني 2012 الى نهاية شهر نيسان 2013.وقد جمعت عينات الدراسة من دم الحبل السري للاطفال الرضع المولودين حديثا اثناء الولادة في مستشفى الاطفال والولادة التعليمي في | The present study was done on new born babies in Diwania province during a period from the beginning of November 2012 till the end of April 2013. Blood samples were collected from the umbilical cord of the new born babies during delivery in children and maternity teaching hospital. Fifty samples were collected separated to serological tests and for blood pictures. Serological tests were used in this research as follows (Rapid IgG - IgM test, latex test and ELISA IgG, IgM). The results showed the Rapid test was the easiest and cheapest test for diagnosis specific antibodies against Toxoplasmosis, therefore the positive samples were 14% for IgG, 4% for IgM and 36% for overlap cases. While the positive results for latex test was 14% but the highest result was recorded by using ELISA test 30% for IgG, No positive result recorded for IgM. There were variation in positive cases of different ages of mothers, the highest percents found in ages between 30 - 39 years old by using Rapid test 72.72% , while by using The latex test demonstrate high percent of infection was 20% and also for ages between 15 - 19 year old, and the ELISA recorded 50% and the same group of age 15 - 19 years old. The results showed the for the residence factor by using Rapid test showed the rural people were high percent of incidence of Toxoplasmosis 57.14%, but the urban people recorded Latex test and ELISA)17.24%, 31.03%) respectively. Aborted women in previous time were followed by using the serological tests Rapid test 10%, Latex 10% and ELISA zero. The incidence of Toxoplasmosis was high in housewives more than other career, the results were) 34.14%, 34.14 (% for Latex and ELISA, IgM and overlapping cases, while in other career by using Rapid test 66.66% Results showed standard Blood parameters did not register significant differences (P> 0.05) for the total number of white blood cells WBC, hemoglobin Hb, blood volume compact PCV, Erythrocytic RBC, and differential count of white blood cells for Neutrophiles, Lymphocyte, Monocytes, Basophiles and Eosinophiles. Phagocytic activity was prominent in adult people more than newly born babies.

تحديد بعض المعادن الثقيلة والتلوث البكتيري في الحليب الخام والمستورد المجفف == Determination of Some Heavy Metals And Bacterial Contamination of Raw And Imported Powder Milk

اسم المؤلف: مروة صباح طالب
اسم المشرف: محمد نافع علي العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: يتعرض الحليب للعديد من الملوثات الفيزيائية والكيميائية والبيولوجية وذلك بسبب طرق الانتاج واسلوب النقل وعمليات التسويق ومن هذه الملوثات المعادن الثقيلة والرقم الهيدروجيني ودرجة الحرارة والبكتيريا. وقد صممت الدراسة الحالية لفحص عينات للحليب الخام التي جمعت | Milk is subjected to various physical, chemical and biological pollutants duo to producing, transporting and marketing processes such as certain heavy metals, pH, temperature and bacteria. The current study was designed to examine fresh milk samples were collected randomelly at early morning from six different locations at a rate of once each month during study period which commenced in October 2013 and ended in march 2014 and powder milk brands which were examined again for six months as in case of fresh milk where these milk brands were collected from local markets each month. The results of heavy metals showed that : • The investigation of Lead content in raw milk revealed that the highest mean value (1.801±0.311 ppm) was scored in Abo - Ghraib sample and the lowest mean value (0.941±0.104 ppm) was observed in Ghazaliya sample. For the Cadmium content in raw milk that the highest mean value (1.532±0.124 ppm) was found in Abo - Ghraib sample and the lowest mean value (0.063±0.044 ppm) was found in Fal'loga. The Copper content in raw milk also investigated and resulted the highest mean value was (0.931±0.092 ppm) in Azizia milk sample and almost similar value (0.931±0.301 ppm) was found in Madain sample while the lowest mean value was (0.308±0.029 ppm) in Fal'loiga sample. The highest mean value of Potassium in raw milk was recorded in two samples in Azizia (9718.8±246.6 ppm) and (9718.3±482.2 ppm) in Abo - Ghraib but the lowest mean value (4156.3±268.3 ppm) was recorded in Essaouira. Regarding Sodium content in raw milk, the results have found that the highest mean value was (2968.8±312.2 ppm) in Essaouira sample while the lowest mean value was (1432.5±156.8 ppm) in Abo - Ghraib. The Chloride content in raw milk samples, the highest mean value was (3053.0±128.6 ppm) recorded in Fal'loga sample and the lowest mean value was (639.0±198.4 ppm) was found in Azizia sample. Analysis of variance of these data showed significant effects (P?0.001) of both sampling sites and collecting months on milk (pb, Cd, K, Na and Cl) contents while analysis of variance of these data reveals no significant impact of sites on milk Cu content (P> 0.05) where no differences were observed between mean value of different collecting sites but shows highly significant (P? 0.001) effects of collecting months.• While powder milk has shown that the highest mean values of Lead (1.225±0.052 ppm) were found in Al - Mudhish brand and the lowest mean value (0.088±0.002 ppm) was detected in Anchor. The highest mean Cd value was (0.184±0.005 ppm) in Nido brand and the lowest mean value was (0.069±0.003 ppm) in Altunsa sample. While the highest mean value of Copper was (1.656±0.254 ppm) in Dielac powder brand and Anchor milk brand had the lowest copper mean value which was (0.767±0.014 ppm). The concentration of Potassium was (9625.0±462.9 ppm) which is the highest recorded in Dielac milk sample and the lowest mean value (3356.8±225.6 ppm) was found in Al - Marai milk sample. While the highest average of Sodium was (2625.0±342.6 ppm) scord in Dielac brand and the lowest average was (1745.0±338.4 ppm) recorded in Al - Marai sample. The highest mean value of Chloride (193.0±41.0 ppm) was recorded in Nido milk brand and the lowest mean value (122.7±22.2 ppm) was found in Altunsa milk sample. However, analysis of variance shows significant differences (P?0.001) between examined milk brands while no such differences were found between these brands (P>0.05) regarding collecting months.From the above results, it seems that the highest content of (Pb, Cd and Cu) are higher than those of standards of the International Dairy Federation (IDF) while contents of (K, Na and Cl) are within the standard requirement according to National Research Council (NRC).Microbial tests of raw milk samples were positive for bacterial growth, as different bacterial species were observed such as Lactobacilli spp. 63.8%, Streptococcus spp. 61.1%, Staphylococcus aureus 44.4%, Escheritia coli 88.8%, Bacillus spp. 72.2%, Salmonella typhi 72.2%, Staphylococcus epidermidis 30.5%, Pseudomonas spp. 47.2%, Micrococcus spp 27.7%, Clostiridia spp. 66.6%, Enterococci spp. 11.1% and K. pneumonia 75% from bacterial contamination of raw milk samples.However, only (11.1%) of the total collected samples of raw milk were suitable for human consumption and (88.9%) unsuitable, indicating that the source of infection or contamination of milk may be due to the animal health, human handler and the environmental factors, e.g., contaminated vessels, polluted water, flies and dust, etc… Obviously, bacterial contamination test of powder milk samples was carried out and negative results were obtained. However, it seems clearly that the opportunity of finding bacterial contamination in powder milk samples may be very rare since milk powder is produced in well designed and modern techniques. Nevertheless, a single bacterial contamination test has indicated to a positive growth of Staphylococcus aureus colonies.

تاثير المجال الكهرومغناطيسي في بعض صفات النمو والحاصل والانقسام الخلوي لنبات العصفر Carthamus tinctorius L == The Effect of The Electromagnetic Field On Some Growth Characteristics And Yield And Mitotic Index For Carthamus Tinctorius L

اسم المؤلف: مروة قيس ابراهيم العبيدي
اسم المشرف: ماهر زكي فيصل الشمري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اولا : - التجربة الحقلية : اجريت التجربة في الحقل التابع لقسم علوم الحياة - كلية التربية للعلوم الصرفة - ابن الهيثم، وصممت باستخدام تصميم القطاعات الكاملة المعشاة Randomized Complete Blocks Design (RCBD) حيث تم تعريض البذور قبل الزراعة لمجال كهرومغناطيسي | First : - Field experience : the experiment was conducted in the Department field of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using design Full sectors Randomized Complete Blocks Design (RCBD) where the seeds exposing to electromagnetic field in the Department of Physics - Electrical Laboratory - College of Education, Pure Science - Ibn al - Haitham before planting them in five intensities (0, 5, 10, 15, 20) mT and two (1.2 and 3) an hour for each intensity to see the electromagnetic field effect in some of the qualities of growth and yield plant Safflower the results were as follows : The effect of electromagnetic field strength was positive traits and was the highest increase in exposure to the intensity of 10 mT for each of the (percentage of germination, root size of the second date, plant height, leaf area, leaf area guide, the dry weight of the root system at the time the second, fresh weight of shoots at the first appointment, the average absolute growth, the sustainability of biomass, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (81.21%, 18.33 cm 3, 93.37 cm, 71.29 cm 2, 1.187, 8.21 g.plant 1, 155.13 g.plant - 1, 1.76 g.yum 1, 691.67 g, 2.03%, 0.733%, 2.47%, 12.96%, 56.96 spad, 44.55 Nora.plant - 1, 46.60 g.plant - 1, 5.35 g, 161.80 kg. acres - 1, 38.74 mg. plant - 1, 12.09%, 13.50%), respectively, except for the size of the root first date, fresh weight of the total root for the first two dates, and second, the dry weight of the root system in the first date, fresh weight of shoots in the second deadline, the dry weight of shoots for the first two dates, and second, the specific weight of the oil had the highest increase at exposing the seeds to the intensity of 15 mT and average (15.16 cm, 15.12 g. Plant - 1, 26.00 g plant - 1, 3.98 g plant - 1, 411.08 g.plant - 1, 20.03 g. Plant - 1, 69.15 g. Plant - 1, 1.069%, respectively) and the highest increase of the proportion of total oil when exposure to the severity of 5mT an average 33.94%. The effect of the length of time in field was significant in most of the qualities which gave the term two highest increase with the exception of germination percentage was the highest increase in exposure for a period of three hours, with an average (60.79%, 91.01 cm, 67.51 cm 2, 1.124, 131.93 g. Plant - 1, 337.61 g. plant - 1, 1.68 g. Day - 1, 1.92%, 12.04 g. plant, 55.99 Spad, 146.06 kg. dunams - 1, 4.77 g, 40.23 mg. plant - 1, 28.03%) for each of the (germination percentage, plant height, leaf area, leaf area guide, fresh weight of shoots in the first date, fresh weight of shoots in the second deadline, the average absolute growth, nitrogen, protein, chlorophyll, yield seeds, 100 - seed weight, carbohydrate content, the total percentage of oil). As was the overlap between field strength and duration significant effect on most of the traits with the superiority of treatment (10 mT for two hours) on the rest of the other transactions for each of the (germination percentage, the size of the root of the first two dates, and the second, plant height, number of branches, leaf area, area guide paper, fresh weight of the root system of the first two dates and II, , dry weight of the root of the first two dates, and the second, fresh weight of shoots for the first two dates, and second, the dry weight of shoots for the first two dates, and second, the concentration of nitrogen, phosphorus, potassium, protein, chlorophyll, the number of inflorescences, He yield the plant, 100 - seed weight, yield seeds, carbohydrate content, the concentration of nitrogen, protein) and average (39.33%, 16.50 cm, 19.50 cm, 103.86%, 17.00, 79.83 cm, 1.330, 16.43 g.plant - 1, 29.20 g. plant - 1, 4.16 g.plant - 1, 10.48 g.plant 1, 184.06 g. plant - 1, 416.33 g. plant - 1, 22.35 g. plant - 1, 73.25 g. plant - 1, 2.10%, 0.800 %, 3.04%, 13.15%, 60.31 spad, 49.14 Nora.plant - 1, 53.37 g. plant - 1, 6.14 g 185.97 kg. acres - 1, - 1.plant 47.81 mg, 2.28%, 14.25%), respectively, with exception each of the average of the absolute growth and sustainability of biomass, where the highest increase in exposure to the intensity of 10 mT for a period of three hours and average (1.85 g.yum 1, 724.85 g.yum), respectively, and total percent of the oil was the highest increase in exposure to the intensity of 5 mT and for three hours and an average of 37.28% and the intensity of 15 mT for a period of three hours, specific gravity of oil and an average of 1.081%Second : - laboratory experiment : experiment was conducted in the laboratory of physiology of the plant Higher Studies - Department of Biology - College of Education, Pure Science - Ibn al - Haitham, and designed using the full random design Completely Randomized Design (CRD) to study the electromagnetic field effect five intensities (0, 5, 10, 15, 20) mT and two (1.2 3) per hour on the severity of the cell division of the safflower plant results were as follows : The results showed that there is a positive effect of the electromagnetic field on the traits and was the highest increase at exposing the seeds to the intensity of 10 mT for each of the (number of dividing cells of the tops of developing the parties to the roots, the total number of cells to the tops of the parties to the roots, and guide cell division developing the tops of the parties to the roots) and average (156.00% 1143.00%, 12.17%), respectively. The effect of duration of exposure, and had a significant effect on the average number of total cells to the tops of the parties to the roots reached 1042.20% when the exposure for two hours.The effect of overlap between the severity and duration of the electromagnetic field significant was effect in all cellular qualities and was the highest increase in exposure to the intensity of 10 mT for a period of one hour and average (167.00%, 13.52%) for each of the number of dividing cells developing the tops of the parties to the roots and guide cell division to the tops of developing the parties to the roots, The total number of cells to the tops of the parties to the roots was the highest increase in exposure to the intensity of 10 mT for two hours and an average of 1203.50%.

التحري عن جين الاوتولايسين في المكورات العنقوديه البشروية المقاومة لمضاد الفانكومايسين == Autolysin Gene Detection In Vancomycin Resistant Staphylococcus Epidermidis

اسم المؤلف: مريم خميس عبد ربة بريس
اسم المشرف: مي طالب فليح
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: من مجموع مئة عينة سريرية جمعت من مصادر مختلفة شملت الحروق والدم والجروح ومسحات انفية, تمكنت 90 عزلة من النمو على اكار المانيتول الملحي , ومن بينهم 40(44.4 %) عزلة عائدة لبكتريا المكورات العنقودية المنتجة للانزيم المخثر للبلازما و50 (55.5%) عائدة لبكتريا ا | Out of one hundred clinical samples were taken from different sources which include burns, blood cultures, wounds and nasal swabs infections ; 90 isolates developed growth on mannitol salt agar. Among these, 40 (44.4%) were Coagulase positive (Staphylococcus aureus) isolates, 50 (55.5%) belong to coagulase negative staphylococci in which Staphylococcus epidermidis isolates were 30(60%). The pattern of antibiotic susceptibility of Staphylococcus epidermidis isolates to 12 antibotics (Amoxiclav, Ceftazidim, ciprofloxacin, clindamycin, Erythromycin, Gentamycin, Imipenime, Penicillin G, Tetracycline, Rifampin , Methicillin, and Vancomycin) were determined using disc diffusion method. The results revealed that resistance to Penicillin G10 and Amoxiclav (Amoxicillin - clavulanic acid) were 100%, Methicillin were 93%, Erythromycin were 90%, Gentamycin and Clindamycin were 70%, Tetracycline and ceftazidim were 75%, Ciproflaxacin were 60%, Rifampin were30%. 95% of S.epidermidis isolates were sensitive to Imipenim and 5% of them were intermediate resistant, while these isolates showed 90% sensitivity to vancomycin. 19 isolates were multidrug resistance. Minimum inhibitory concentration of S.epidermidis isolates to vancomycin, was determined. The results revealed that (12) S.epidermidis isolates (40 %) were vancomycin resistant, the MIC of them were between 256 ?g\ml and 32 ?g\ml, (4) S.epidermidis isolates (13.3%) were intermediate resistance, the MIC to 3 of them were 16 ?g\ml and the last was 8 ?g\ml. Some virulence factors of VRSE and VSSE were detected including the hemolysin, protease, lipase and urease. The S. epidermidis isolates were produce hemolysin, protases, lipase and urease, were 100%, 100%, 25%, 100% respectively in VRSE, while in VSSE 100%, 100%, 60%, 100% respectively. The isolates were subjected to polymerase chain reaction (PCR) technique in monoplex pattern to amplify resistant incoding gene : the vanA, vanB and autolysine gene aae gene. The results by this study showed that 12 (40%) S. epidermidis isolates gave the implicone size (1030 base pair) of the vanA gene. However the results of MIC and PCR were similar but no any isolates gave product for presence of vanB gene. All S.epidermidis were able to produce implicone size(858bp) of aae gene. The effect of vancomycin resistant S. epidermidis on cell autolysis activity was detected by whole cell autolytic assay.The results revealed that there was significant difference among three isolates, the VSSE isolate (S.epidermidis 22) have the highest autolytic activity in the presence of antibiotic, followed by the VRSE isolate (S. epidermidis 1) and the VISE isolate (S. epidermidis14) which was the lowest autolytic activity with the presence of antibiotic. The result of transmission electron microscope (TEM) showed that the VRSE isolates (S.epidermidis 1) have thicker cell wall followed by VISE (S.epidermidis 14) isolates.However, the VSSE (S.epidermidis 22) didn't showed any cell wall thickening.

دراسة مقارنة للكشف عن الجيارديا لامبليا والطفيليات المصاحبة لها بين الاطفال في مدينة كركوك باستخدام بعض التقانات المختبرية == A Comparative Study For Detecting Giardia Lamblia And Associated Intestinal Parasites Among Children In Kirkuk City By Using Some Laboratory Methods

اسم المؤلف: مها اسماعيل مصطفى الجبوري
اسم المشرف: يحيى جرجيس سلمان
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: كركوك
الصفحات الاولى:
المستخلص: The current study had been carried out from January 2013 to July 2013 in medical laboratory researches - Kirkuk Faculty of Medicine. A total of 310 stool samples have been collected from children suffering from liquid diarrhea, their ages are from below one year to 12 years; six different laboratory diagnostic methods have been applied for detecting Giardia lamblia and other intestinal parasites. For microscopy diagnosis; direct wet double prepartions, zinc sulphate flotation are used. While immunological methods involve; Enzyme Linked Immuno - Sorbent Assay, corpo - antigen (ELISA), Direct Fluorescent Assay (DFA) and Lateral immune - chromatography assay (Triage panel). Giardia genome amplification is done using conventional Polymerase Chain Reaction (PCR) single step procedure by using of mixed primers of Giardia assemblages A1, A2 and B. The total rate of intestinal parasitic infection is 51.93 % distributed in 161 stool samples. This rate involve high frequency of protozoan infection in 132 (42.58 %) compare to 29 (9.35 %) for helminthes, P<0.05. More common intestinal protozoan parasites were Giardia lamblia 63(20.32 %) followed by Blastocyst hominis, Cryptosporidium parvum, Entamoeba coli, Entamoeba histolytica, Iodomoeba butschili, Endolimax nana and Balantidium coli with the rates : 6.77%, 6.45%, 4.18 %, 2.58 %, 1.29 %, 0.64 % and 0.32 % receptively. Concerning intestinal helminthic infection, high number and rate 23(7.41 %) was with Hymenolepis nana is compare with 1(0.32 %) record for Ancylostoma duodenale.According to gender high rate of giardiasis is recorded among males than in females, conversely to high rate of other intestinal parasites among female than in males P<0.05. Statistically relationship between Giardia distribution and ages are not significant P>0.05. Giardia co - existence are highly detected with Balstocystis hominis, and Cryptosporidium parvum total Giardia mixed parasitic infection rate 8.06 % is lower than12.23 %of pure Giardia lamblia infection, P>0.05. Regarding Giardia lamblia detecting according to laboratory methods; high rate of giardiasis 22.29% is reported by using PCR technique, followed by 20, 23 % by using direct wet preparation technique, P<0.05.The efficacy of laboratory methods for detecting Giardia stages are reduced; the following rates 19.35%, 19.03%, 17.74% and 14.51% obtained by using ELISA, DFA, flotationand lateral immuno - chromatography assay (Triage) respectively, P<0.05. Also statistical analysis reveals significance of PCR sensitivity, specificity, and accuracy in detecting giardiasis than other laboratory methods. Negative predictive values NPV in relation to type of laboratory methods are high, but statistically they are not significant, controversy to positive predictive value PPV that are significant. The efficacy of Triage panel is high for detecting giardiasis 14.45 % as compare with 4.5 % and 3.5 % for detecting Cryptosporidium parvum oocysts and Entamoeba histolytica respectively P<0.05 %. Considering the application of double direct wet preparations, the results of using this method are beneficial for detecting protozoan and helminthic parasites. While the using of zinc sulphate flotation technique reveal fluctuated results in spite of significant statistical analysis P<0.05. The employee of five laboratory methods for detecting the oocysts of Cryptosporidum parvum; the following rates 6.45%, 5.48%, 4.83%, 4.51% and 3.22% are recorded with the usage of DFA, direct microscopy, modified Ziehl - Neelsen method, Traige panel and flotation method respectively, P>0.05. Giardia lamblia DNA extraction from 80 stool samples that amplified using Giardia gene loci K725, reveal 66 samples positivity, pure Giardia lamblia genomic mass mean is 437.56 bps, with 1.705 % of genome purity and the extension of genomes range from 280 to750bps.While 23 of mixed Giardia plus other protozoan parasites, the mean of gemonic mass is 439.89 bps with genome purity mean 1.56 %. Amplification of Giardia genome mixed with helminthes reveal 443.33 bps of genomic mass mean and 1.49 % genome purity mean. In general the all genomic mass of Giardia lamblia (Pure and mixed infection) is 440.26 bps and purity mean 1.54 % P>0.05.PCR amplification in stool sample exert that Giardia lamblia genomes are mixed of human and animal type.

دراسة انتشار الحيوانات الابتدائية الرئيسية المسببة للاسهال بين المرضى باستخدام الطرائق المجهرية والجزيئية في محافظة بابل == Prevalence Study For Main Protozoa Diarrheal Agents Among Patients By Using Microscopically And Molecular Methods In Babylon Province

اسم المؤلف: ميس كاظم عليوي
اسم المشرف: احمد خضير عبيس الحميري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية خلال المدة من تشرين الاول 2014 الى شهر شباط 2015 حيث تضمنت هذه الدراسة فحص 987 عينة براز (اطفال وبالغين, ذكورو اناث) بطريقة الفحص المجهري المباشر و96 عينة موجبة فحصت عن طريق تقنية تفاعل سلسلة متعدد البلمرة. للمرضى المصابين بالاسهال و| The current study during period was conducted from October 2014 till February 2015 examination of 987 stool samples for direct smear method (Lugol's Iodine, Normal Saline (0.9%) for detection of the following parasites G.lamblia, E.histolytica while using floatation methods and Ziehl - Neelsen method (Malachite green) for detecting the Cryptosporidium spp. by using light microscope) and 96 positive samples from these samples examined by polymerase chain reaction technique PCR. For patients infected with diarrhea (children and adults, male and female) who attended to Babylon maternity and children hospital and specialized Marjan Hospital for Internal and Cardiac Diseases in the Babylon province as well as primary health care and private clinics. The age ranges from(Less than one year - 31and more).The current study showed the rate of infection with parasites that causative of diarrhea47.3% (E.histolytica, G.lamblia and Cryptosporidium spp.) was 26.4%, 17.9% and 3.7%, respectively.They were examined by a direct smear method to detect the trophozoites, cyst and oocyst phases of these parasites. The highest rate of infection by microscopic examination was in the rural area 67.2% in comparing with city that was 32.9%. Also the highest rate of infection among males was 51.5% in comparing with females 41.2%. It has been recorded that the higher rate of infection was 76.1% in the age group (16 - 20) years while the lower infection rate was in the age group (26 - 30) years that was 22.8%. It has been observed significant differences in infection rates at the (P ? 0.05). The present study recorded difference in the rates of parasitic infections according to the presence of animals in houses, the high rates of infection where with those have animals in their houses 51.2% while the lowest rates of infection where with those not have animals in their houses 38.9%.It has been observed significant differences in infection rates at the (P ? 0.05). This study reveals a difference in parasitic infections rates, according to the level of education the head of household, and found that the highest rate of infection for those with the head of the family is non - educated or illiterate 48.3%, while the rate of infection was declined with head of the family who get primary education level 46.2%. It had recorded the highest infection rate in October 63.8%, while the lowest percentage was in February 27. 6%. It has been observed significant differences in infection rates at the (P ? 0.05). In the present study Polymerase chain reaction (PCR) is used to 96 positive results in direct smear methods to detection the main parasitic diarrhea agent. It had recorded total of infection rate of 43.4% (31.3%, 28.1% and 2.1%, respectively). Depending on PCR technique showed the highest rates of infection was in the male 36.7% while the lowest rates of infection in females 30.6%. It had recorded the highest rate of infection in the rural areas 45.3% in comparing rate of infection in the urban areas 25.9%. The present study recorded the highest rates of infection were in the (16 - 20years) age group 46.2%, while the decline in the rates of infection was in the (21 - 25years) age group 16.7%. Also it showed the highest rates of infection were in the presence of animals in the houses 36.1% while the lowest rate of infection where there were no animals 31.4%.It has been observed significant differences in infection rates at the (P ? 0.05). The study found that the highest rates of infection for those with the head of the family is non - educated or illiterate 38.9%, while the rate of infection declined with head of the family who got Academic education level 33.3%. Also it had recorded the highest rate of infection in November (2014) 42.1%, while the lowest rates percentage was in December (2014) 23.1%. It has been observed significant differences in infection rates at the (P ? 0.05). Through the current study it was concluded that the prevalence of the parasites that cause diarrhea in the Babylon province are very highly when detection microscopic examination and PCR method, comparison with previous studies and rural area highest rates of infection from urban area.
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