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دور الفيروس المضخم للخلايا CMV في مرضى التهاب الكبد الفيروسي ذاتي المناعة في حالات التهاب الكبد المزمن نمط B == The Role of CMV In Autoimmune Hepatitis Among Chronic Cases of Hbv
Author name:
هـدى جميل باقر الخلخالي
Supervisor name:
محمد عبود محسن | محمد عبد كاظم السعدي
General topic:
Biology
Specific topic:
Microbiology - Viruses
Degree:
Doctorate
University:
University of Kufa - College Of Science - Department Of Biology
Language:
English
University location:
Najaf
First pages:
24T2677 - p.pdf
Abstract:
هدفت الدراسة الحالية الى تشخيص التهاب الكبد الفايروسي نمط (ب) وتشخيص التهاب الكبد ذاتي المناعة في المرضى المصابين بالتهاب الكبد.ودور الاصابة بالفايروس المضخم للخلايا البشرية CMV في حث التهاب الكبد ذاتي المناعة.من مجموع 360 حالة مشبه بها جمعت من مستشفى ال | This study aimed to investigate the existence of hepatitis B virus and autoimmune hepatitis in hepatitis B patients as well as to detect the role of cytomegalovirus in the induction of AIH disease. A total of 360 suspected cases were collected from Center Health laboratory/Al - Hakeem Hospital, and AL - Sadder medical city in AL - Najaf city, during the period from January (2013) to August (2013). Only 76 were seropositive hepatitis B (55 males and 21 females with age ranging 11 - 72 years).In addition, 15 healthy individuals without any evidence of chronic inflammatory disease were depended on the control group, age ranging 21 - 50 years.Blood samples were collected from patients and healthy controls were tested for HBsAg and Anti - HBc Ab using Enzyme Linked Immunosorbent Assay (ELISA) technique to investigate hepatitis B seropositive and chronic hepatitis B respectively. For investigated AIH disease was performed depending on the Line Immune Assay technique. While for detection CMV were initially identified by serological technique (ELISA, MiniVIDAS) to detect anti - CMV IgM and anti - CMV IgG; then confirmed employment molecular technique using Real Time Polymerase Chain Reaction for the detection the presence of DNA of CMV. Samples were collected from patients and control to estimation immunological level (C3, C4, IL - 10 & TFN - ?) by using ELISA and radial immunodiffusion method.The results showed that 76 HBsAg seropositive in all age groups but the age group 44 - 54 year revealed high significance (p<0.05) than other age groups. While 35 out of 76 seropositive with HBc Ab, the age group 55 - 65 years showed high significance (p<0.05) than other age groups, and males more infection than females. The result also revealed that the AIH disease was 5 out of 76 patients infected with Type 1 AIH. Included 2(40%) have demonstrated the infection HBsAg positive with reactivation CMV While 3(60%) infected with HBsAg only.The results showed that 68 out of the 76 samples were positive for anti - CMV IgG antibodies, and 4(5.26%) samples were positive for anti - CMV IgM. While the MiniVIDAS test results showed 73 out of the 76 samples was positive for anti - CMV IgG antibodies. 2(2.6%) were positive for anti - CMV IgM antibodies. The results of the Real - Time PCR revealed that DNA of CMV were detected in 23 out of 76 patients were found in all age groups with viral loads ranging from (0.24 - 1730000) Copies/ml, and the results of controls group in Real - Time PCR were CMV negative. The results of cytokines (TNF - ?) showed a high significance (P<0.05) elevation in the serum of all patients than control (419.3 ± 27.8) pg/ml, and the results that AIH showed increase in cytokine level was (1218.2±44) pg/ml than other patients. Whereas chronic hepatitis B patients recorded high significance (P<0.05) in level (IL - 10) was (901.5±22.2) pg/ml than other patients and control (373 ±30.3) pg/ml. According to sex no - significance difference between males and females in results of cytokines profile (IL - 10, TNF?). Complement fraction C3 decreased in all patients compared to those healthy control, while the AIH patient recorded high significance (P<0.05) 142.2±8 mg/dI than the other patients. In regard to C4 was revealed normal level in all patients compared with control groups while in the AIH revealed high significance (P<0.05) was 41.7±5.1 mg/dI compared with other patients and no - significance difference (P<0.0) between males and females in level of C3and C4.The overall finding results showed that the activation cytomegalovirus with hepatitis B virus Contribute to the induction of AIH and cause immune suppressor for them by increase and decrease many immune factors.
