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تقييم وعزل فيروس الانفلونزا للانسان ودراسة الاستجابة المناعية في الجرذان التجريبية == Evaluation And Isolation of Human Influenza Virus And Studying Their Immune Response In Experimental Rats
Author name:
فاديه مهدي مسلم العبيدي
Supervisor name:
عبد المجيد عبد الله السعدي | يونس عبد الرضا الخفاجي
General topic:
Biology
Specific topic:
Microbiology - Viruses
Degree:
Doctorate
University:
University of Kufa - College Of Science - Department Of Biology
Language:
English
University location:
Najaf
First pages:
24T2699 - p.pdf
Abstract:
This study has been conducted for the first time in Najaf / Iraq, and the study included the preparation of a local inactivated vaccine (whole and subunit).The number of cases infected with seasonal influenza virus were 647 cases out of one thousand suspected case. They were distributed into eleven groups. Seasonal influenza virus was detected by two diagnostic methods (rapid test device and real time PCR).The present study has reflected that the most diagnosed cases infected with seasonal influenza virus during the period extended from March 26, 2012 up to April 30, 2013 represented by were type A (H3N2) (283) cases. Whereas H1N1 (148) cases, H3N2+H1N1, (56) cases, H1N1 + H3N2 + B were (30) cases and 130 cases were infected with type B.Current study also revealed that age group of 46 - 60 years old infected with seasonal influenza virus were (159) case including (368) male and females were (279) of different infected group..The spread of seasonal influenza virus in Najaf varies from one region to another depending on the population density. Clinical cases were collected randomly during the months of the year. It has been found that the spread of disease has something to do with temperature and humidity as high humidity and low temperature lead to increased spread of the disease because of their relationship at the stage of the spread of epidemic influenza that spread from Dec 26, 2012 and up to April 1, 2013 in addition to expatriates infected..Two techniques were used for isolation of seasonal influenza virus first inoculation of embryonated chicken egg and secondly chicken fibroblast cell culture.It was found that at the embryonated egg lethal dosage (ELD 50 / 0.5 ml) (109.6) whereas tissue culture infective dosage (TCID50 / 0.1 ml) was (109.3) of (H1N1+ H3N2) and type (B) virus..Virus was purified by two ways that are depending on the vaccine type. The gamma irradiation that dosage used for inactivating vaccine of the purified virus which was extracted from allantoic fluid and tissue culture was (5.702) mSv/h for subunit virus vaccine type whereas gamma irradiation dosage for complete (whole) virus vaccine was (8.816) mSv/h. The results proved that immunoglobulins (IgM, IgA and IgG) levels as well as (IL - 17, IL - 10 and INF - 1?) indicated significant differences among vaccinated, infected and control groups.Subunit and whole virus vaccine extracted from chicken fibroblast cell culture are considered the best of derived from virus propagated in embryonated chicken eggs.Subunit virus vaccine is better than the whole virus vaccine derived from virus propagated in embryonated chicken eggs and extracted from chicken fibroblast cell culture.The current study also reflected that locally prepared virus subunit vaccine and purified whole virus killed vaccine capable of inducing humoral and cellular immune response in experimentally vaccinated rats..Histopathological changes appeared clearly on tissue sections (lung, trachea, heart, liver, spleen, kidney) of the infected group as compared with control and the vaccinated groups.
