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تشخيص طفيلي المشعرات المهبلية بتقنية تفاعل البلمرة المتسلسل RT - PCR في محافظة واسط - العراق == Diagnosis of Trichomonas Vaginalis Using Real - Time Pcr Technique In Wasit Province - Iraq
Author name:
رسل واسط كاظم
Supervisor name:
عبد السادة عبد العباس راهي
General topic:
Biology
Specific topic:
Microbiology - Parasites
Degree:
Master
University:
Wasit University - College Of Science - Department Of Biology
Language:
English
University location:
Wasit
First pages:
24T2746 - p.pdf
Abstract:
في الدراسة الحالية، نصف طريقة سلسلة تفاعل البلمرة المتسلسل للكشف عن DNA محدد من طفيلي المشعرات المهبلية في الفحص السريع لتقنية الوقت الحقيقي لسلسلة تفاعل البلمرة. وتقييم مدى انتشار داء المشعرات وفقا مع دراسة العوامل المختلفة التي تؤثر على داء المشعرات الم | This study was involved the describe of an existing PCR method for specific detection of Tichomonas vaginalis DNA into a rapid real - time PCR assay, and to evaluated the recent prevalence of trichomoniasis accordance with studying different factors that affect on vaginal trichomoniasis in females complaining of vaginal discharge.Vaginal swab samples were collected from 401 patients attending two public hospitals and three private clinic in the Al - Kut city. Demographic data, clinical and socioeconomic status were collected from the patients using a structured questionnaire. Total genomic DNA was isolated from 60 samples of vaginal swab randomly chosen to specific detection of T. vaginalis DNA into a rapid real - time PCR.All samples were examined by the direct microscopic examination (wet mount and Giemsa - stained smear). Their ages were ranging from 14 - 63 years. The vaginal swabs examined by wet mount and Real Time - PCR to detect the presence of T. vaginalis. The pH strips were used to detect the vaginal acidity.Of the 60 women, 13 were positive by Real Time - PCR giving the prevalence rate of (21 67%) with sensitivity, specificity and accurate rate 100% and the direct microscopic examination (wet mount and Giemsa - stained smear) show 5/60 (8.33%) positive case while 55/60 (91.67%) gave a negative result with a sensitivity and specificity, and accuracy (38.5%) (100%) (86.7%).Women aged (24 - 33)years had significantly higher prevalence of trichomoniasis (46.1%) than other age groups.Married women had the highest percentage of trichomoniasis (76.9%), comparing with widowed women (7.7%) and divorced women (15.4). With statistical significant difference between these groups. Uneducated women had significantly higher rate of T.vaginalis infection (76.9%) than educated women (23.1%). Statistical significant difference was detected between these groups.Pregnant women showed a higher infection rate 7(53.8%) than non - pregnant 6 (46.2%).Women with vaginal pH (6 - 7), (5 - 6) had significantly higher rate of infection (53.8% and 38.5%) respectively.Women who used (loop and the oral contraceptive pills) contraception had the lower rate of infection(23.1%) than those not using any contraception (67.9%). Statistical significant difference was detected between these two groups.The highest percentage 2(66.67%) was found among women with T. vaginalis who used contraception for >2 years.The higher percentage of infected with T. vaginalis parasite was found in bad odor or fish odor vaginal discharge which was noted (76.9%) of infected women with T. vaginalis. Statistical significant difference was detected between these groups. Women with Frothy discharge color showed higher significantly percentage of infection 7(53.8%) than other with Yellow to green and bloody discharge and Clear discharge 3(23.1%), 2(15.4%) and 1(7.7) respectively.Multipara women revealed the highest rate of trichomoniasis (46.15%). No statistical significant difference was detected between infection and parity.T.vaginalis was more commonly seen in women with frequency of sexual intercourse 2 - 3 time per week 5 (50%) cases. The lowest incidence was detected among those who had sexual intercourse once per month 1 (10%), with statistical significance among these variables. T.vaginalis was found to be predominant among housewives (69.23%) than employees (30.77%).Fertilizing women were showed higher 9(69.2%) T. vaginalis infection than non - fertile women 4(30.8%).The high incidence of T.vaginalis infection was found among rural females 8 (61.5%), followed by urbanized females 5 (38.5%).
