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دراسة مقارنة بين تشظي DNA النطف والموت الخلوي المبرمج لبيوت الطاقة وعلاقتهما بعوامل العقم الذكري == A comparative study between sperm DNA fragmentation and mitochondrial apoptosis correlated to male infertility factors

اسم المؤلف: الاء محمد ياسين
اسم المشرف: عبد الامير ناصر غلوب | محمد باقر محمد رشاد فخر الدين
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تعتبر بيوت الطاقة ((mitochondria والحمض النووي الرايبوزي منقوص الاوكسجين (DNA) للنطف عوامل تنبؤ اساسية لحيوية النطفة المسؤولة عن نجاح الحمل سواء كان بالطريقة الطبيعية او عن طريق التقنيات المساعدة للانجابART) ) . لذلك هدفت هذه الدراسة لتقييم الموت الخلوي المبرمج لمايتوكوندريا النطفSperm mitochondrial apoptosis) SMA ) وتشظي المادة النووية للنطف SDF((Sperm DNA fragmentation. ثم تحديد العلاقة بينهما وبين عوامل العقم الذكري للسائل المنوي, وايضا ايجاد العلاقة بين نتائج فحص Acridinie orange (AO) وفحص الموت الخلوي المبرمج لمايتوكوندريا النطف (SMA). اجري تحليل السائل المنوي ل (85) ذكر, وكان عدد الذكور من ذوي نوع العقم الاولي (52) , ومتوسط اعمارهم (1.42± 32.13) سنة , وعدد الذكور من ذوي العقم الثانوي (33) , وبمعدل عمري قدره (1.85± 35.22) سنة , تم تقسيم الذكور الى عدة مجاميع على اساس عوامل العقم الذكري لسائلهم المنوي حسب معايير منظمة الصحة العالمية لعام (2010) عدا شكل النطف تم حسابه اعتمادا عى معايير منظمة الصحة (WHO) لسنة (1999). ايضا تم اجراء فحص الموت الخلوي المبرمج للمايتوكوندريا (SMA) لجميع الذكور بواسطة عدة تصبيغ المايتوكوندريا , وفحص تشظي المادة الوراثية بواسطة فحص (AO) وفحصت خلايا النطف المصبغة بواسطة (Fluorescent Microscope). اظهرت نتائج هذه الدراسة زيادة معنوية في الموت الخلوي المبرمج لمايتوكوندريا النطف (SMA) بطيئة الحركة التقدمية (Asthenozoospermia), وحالات النطف قليلة العدد وبطيئة الحركة التقدمية (Oligozasthenozoospermia), وحالات النطف قليلة العدد بطيئة الحركة التقدمية ومشوهة الشكل (Oligozasthenoteratozoospermia), بينما اظهر الذكور ذوي السائل المنوي طبيعي النطف والذكور ذوي النطف بطيئة الحركة التقدمية مشوهة الشكل (Asthenoteratozoospermia) والذكور ذوي حالات النطف قليلة العدد مشوهة الشكل زيادة غير معنوية (P>0.05) في نسبة (SMA) بالمقارنة مع المجاميع الاخرى. بالاضافة الى ذلك, اظهرت هذه الدراسة علاقة سلبية معنوية (P<0.05; r= - 0.94) بين الحركة التقدمية الكلية وبين نتائج فحص (SMA) في مجموعة الذكور ذوي النطف المشوهة (Teratozoospermia) , بينما كانت العلاقة سلبية غير معنوية(P>0.05) بين الصفات اعلاه مع تشظي المادة النووية (SDF) . اضافة الى ذلك اظهرت الدراسة الحالية ان الموت الخلوي المبرمج وتشظي المادة النووية كان باعلى النسب في حالات العقم الذكري التي يشترك فيها عامل عقم بطئ الحركة التقدمية. ايضا, تم ملاحظة اختلافات غير معنوية (P>0.05) في تشظي المادة النووية بين مجاميع عوامل العقم الذكري. على اية حال, كان التشظي في اعلى مستوياته عند الذكور ذوي قلة عدد النطف مع بطئ الحركة التقدمية لها, يليها نسبة اقل من (SDF) في الذكور ذوي النطف بطيئة الحركة التقدمية, بينما ظهرت اقل نسبة من (SDF) في الذكور ذوي السائل المنوي الطبيعي (Normozoospermia). استنتجنا من هذه الدراسة ان لفحص الموت الخلوي المبرمج لبيوت الطاقة لخلية النطفة دور مهم في بيان العقم الذكري . اضافة الى ذلك, ان لكلا الفحصين (SMA and SDF ) اهمية تنبا للمرضى ذوي عامل عقم بطئ الحركة التقدمية سواء كان لوحده او مرتبطا مع عوامل العقم الذكري الاخرى. | Sperm mitochondria and sperm DNA considered essential factors to prediction of sperm viability, which is responsible for successful pregnancy in natural way or in assisted reproductive techniques. Therefore, this work aimed to evaluate the sperm mitochondrial apoptosis (SMA) and DNA integrity for infertile patients, then to assess correlation between semen parameters with both SMA and sperm DNA fragmentation (SDF), also to assume the correlation between results of SMA test and acridine orange test. Seminal fluid analysis was performed for (85) male, the number of primary infertile men were (52) with average age (32.13 ± 1.42) year, and number of secondary infertile men were (33) with an average age (35.22 ± 1.85) yearAll patients were classified into several groups according to the male infertility factors according to WHO (2010) criteria with exception for the morphology that assessed according to WHO (1999). Also, the other two tests including SMA and SDF were performed for all infertile patients. SMA was assessed by mito - capture kit assay and the SDF assessed by Acridine Orange (AO) by using of fluorescent Microscope. The results of the present study showed a significant difference (P< 0.05) in SMA assay for asthenozoospermia, oligoasthenozoospermia and oligoasthenoteratozoospermia as compared with teratozoospermia, while normozoospermia, asthenoteratozoospermia, and oligoteratozoospermia had non significant differences (P > 0.05) in SMA (%) as compared with the other groups. Moreover, a significant negative correlation (P<0.05; r= - 0.94) assessed between total progressive motility (TPM) and SMA in Teratozoospermia, while it was negative non significant (P< 0.05) correlation with SDF. Also, this study showed that SMA and SDF were in the higher percentages in males who shares asthenozoospermia male infertility factor. Non significant differences (P>0.05) had been observed among groups of male infertility factors for sperm DNA fragmentation. However, SDF was in the maximum value for male with oligoasthenozoospermia, followed by asthenozoospermia. In contrast, minimum value was assessed in group normozoospermia males. A significant increment has been observed for secondary infertility as compared to primary infertility in sperm concentration, sperm progressive motility (%), sperm normal morphology (%), and total sperm motility (%). Additionally, this study showed that SMA and SDF were in higher values in cases that shares asthenozoospermia. From the results of the present study, it was concluded that the sperm mitochondrial apoptosis assay (SMA) has an important role in investigation of male infertility. Furthermore, both SMA and DFI assays have predictive value for patients with asthenozoospermia either alone or combined with other male infertility factors.

تاثير بعض المحفزات الكيمائية والنانوية في انتاجية بعض مركبات الايض الثانوية من المزارع النسيجية لنبات الاقحوان Calendula officinalis L. == Effect of some chemical and nanoparticle elicitors on the production of secondary metabolites of Calendula officinalis L. In Vitro

اسم المؤلف: اسيل صالح محمدامين
اسم المشرف: هاشم كاظم محمد
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجري هذا البحث الحالي في مختبر زراعة الانسجة النباتية ، قسم علوم الحياة ، كلية العلوم ، الجامعة المستنصرية ، بهدف زيادة انتاج بعض المركبات الثانوية في نبات الاقحوان Calendula officinalis L. خارج الجسم الحي. قدرت مركبات الايض الثانوي بالتحليل الكمي والنوعي باستعمال جهاز كوماتكرافيا السائل ذو الاداء الفائق HPLC وقورنت مع مايحتويه النبات الام. استحث الكالس من الاوراق بعد زراعتها على وسط MS مزود بالتراكيز 0, 1, 2, 3 او 4 ملغم\لتر من 2,4 - D . بينت النتائج ان الوسط الحاوي على 2ملغم\لتر من 2,4 - D اعطى اعلى نسبة مئوية لااستحثاث الكالس بلغت 90% عند التحضين بالضوء, واعطى اعلى وزن طري وجاف للكالس هذه المعاملة 612, 33.8 ملغم على التوالي. وبهدف زيادة المركبات الثانوية اضيفت بعض المحفزات السالسيلك اسيد بتراكيز 0, 50 , 100 , 150 او 200 ملغم\لتر, الفنل النين بتراكيز 0, 10 , 20 , 30 او 40 ملغم\لتر ونترات الفضة النانوية AgNO3 بتراكيز 0, 0.3 , 0.6 , 0.9 او 1.2 ملغم\لتر. اظهرت النتائج ان التركيز 200 ملغم\لترمن السالسيلك اسيد وتركيز 20 ملغم\لتر من الفنيل النين اعطى زيادة معنوية في اغلب مركبات الزيوت الطيارة لنبات الاقحوان بينما اعطى تركيز 1.2 ملغم\لتر من نترات الفضة النانوية زيادة معنوية في جميع مركبات الزيوت الطيارة لنبات الاقحوان. | The present study was conducted in the laboratory of plant tissue culture, Department of Biology, College of Science, University of Al - Mustansiriyah, in order to increase the production of some secondary metabolic compounds in leave of Calendula officinalis L. in vitro. Secondary metabolites were estimated quantitatively and qualitatively using high performance liquid chromatography HPLC and compared with those in the mother plant. Callus was induced from leaf explants and cultured on MS medium containing different concentrations of growth regulators including 2,4 - Dichlorophenoxy acetic acid 2,4 - D at the concentrations 0, 1, 2, 3 or 4 mg/l. Results showed that the medium containing 2 mg/l of 2, 4 - D gave the highest percentage of callus induction reached 90 % under light conditions, the highest fresh and dry weight were recorded 612, 33.8 mg respectively. In order to increase the production of secondary metabolites, some elicitors were used including Salicylic acid at the concentrations 0, 50, 100, 150 or 200 mg/l, phenyl alanine at the concentrations 0, 10, 20, 30 or 40 mg/l, and silver nanoparticles AgNO3 at the concentrations 0, 0.3, 0.6, 0.9 or 1.2 mg/l. Results showed that 200 mg/l concentration of Salicylic acid and 20 mg/l concentration of phenyl alanine caused highly significant production in most of the secondary metabolites (essential oils) of C. officinalis L. while 1.2 mg/l of silver nitrate nanoparticles AgNO3 caused

دراسة بكتريولوجية وجزيئية للجين norA في بكتريا Staphylococcus species المقاومة للمثيسيلين == Bacteriological and molecular study of norA gene in Methicillin Resistant Staphylococcus species bacteria

اسم المؤلف: احلام خليفة عبد الله التميمي
اسم المشرف: محمد فرج شذر المرجاني
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: One hundred clinical isolates of methecillin resistant Staphylococcus spp. were collected from different sources including : (80,10,5,3,2) isolates from wounds, blood, urine, pus, otitis media respectively from different hospitals in Baghdad during the period from 1/9/2013to 1/1/2014. All isolates were identified through morphological, cultural and some biochemical testes. Coagulase test was performed done by using both tube and slide method The results showed that 75% of isolated were positive for this test which refer to Staphylococcus aureus, and 25% were Coagulase negative Staphylococci (CoNS) .The antibiotic susceptibility test against 10 antimicrobials were determined by the agar diffusion method ; Results showed that all isolates were resistant to ceftriaxone, cloxacillin and aztreonam. The most effective antibiotic aganist isolates were ciprofloxacin and norfloxacin. The Minimum inhibitory concentrations (MICs) for ciprofloxacin and norfloxacin were determined by agar dilution method .The MIC isolates ranged from 4 to 1024 μg/ml for both antibiotics. On the other hand the minimum inhibitory concentrations (MICs) were determined for quartenary ammonium compounds and chloroxylenole . Results revealed that MIC for chloroxylenole were 50 - 500μg/ml and 500μg/ml for quartenary ammonium compounds.Thirty Four of methicillin resistant isolates were choosen for detect efflux pumps by using Ethidium Bromide - Agar Cartweel Methode (EtBrCW), which include ethidium bromide dye as marker to detect efflux pumps, the result showed that 18 isolates(52.9%) give positive result, 3 isolates (8.82%) were intermediate, wherase remaining isolates 13 isolates(38.29%) were give negative result.Polymerase Chain Reaction (PCR) technique was performed done using specific primer targeting the specific sequences of the norA gene, The results showed that norA found in 16 isolates (47%). Nucleotide sequence for norA gene in 9 isolates were determined ,results revealed consistency reaching up to 90 % as compared nitrogen bases sequence of the norA gene present in the Staphylococcus aureus strain in NCBI Detection of gene expression was performed done by using q RT - PCR technique after RNA extraction and cDNA syenthesis , the result showed that gene expression were various among isolates , and the gene expression increase with increasing MIC of ciprofloxacin and norfloxacin.The effect of biosurfactant as efflux inhibitors was tested by detection of MIC for ciprofloxacin alone and mixed with biosurfactant , the result showed ability of biosurfactant in decreasing the MIC to two or four - fold in the presence of inhibitor this refer to biosurfactant effective material against NorA efflux pump.

تثبيـط نمو وتكوين الغشاء الحيوي لبكتريا Pseudomonas aeruginosa بالبروتينات المنتجة من خـمـيرة cerevisiae Saccharomyces == Inhibition of Pseudomonas aeruginosa Biofilm formation and Growth by inhibitory Proteins Produced by Saccharomyces cerevisiae

اسم المؤلف: احسان علي رحيم السوداني
اسم المشرف: نبراس نزار محمود
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: جمعت 207عينة سريرية من بعض مستشفيات بغداد ولفئات عمرية مختلفة ولكلا الجنسين للفترة من 15/10/2013 - 15/1/2014 وعزلتﹸ وشخصتﹸ 68 عزلة من بكتريا Pseudomonas aeruginosa ومن مصادر مختلفة شملت (28) عينة من الحروق و(21) من اخماج الجروح و(6) اخماج المسالك البولية و(6) من اخماج الاذن الوسطى و(4) من التهاب البلعوم و(3) من القشع. شخصتﹸ العزلات البكتيرية اعتمادا على صفات المستعمرات المظهرية والمجهرية عند تنميتها في الاوساط الزرعية التفريقية والاختيارية فضلا عن الفحوصات الكيموحيوية ولتاكيد نتائج الفحوصات الكيموحيوية استعمل نظام Vitek2 compact كخطوة تكميلية . استعملت في هذه الدراسة طريقتين للتحري عن تكوين الغشاء الحيوي وهي طريقة Congo Red Agar Method(CRA) وطريقة اطباق المعايرة الدقيقة Microtitration plates method (MTP).اظهرت النتائج ان طريقة (MTP) كانت الاكثر دقة في التحري عن تكوين الغشاء الحيوي , اذ لوحظ ان (28)عزلة من اصل (68) عزلة كانت مكونة للغشاء الحيوي , في حين كانت (40) عزلة غير مكونة للغشاء الحيوي قياسا بطريقة (CRA) التي كانت عدد العزلات المكونة للغشاء الحيوي فيها (16) عزلة و(52 ) عزلة غير مكونة للغشاء الحيوي .اخضعت العزلات المكونة للغشاء الحيوي لفحص الحساسية تجاه (17) مضادا حيويا بطريقة الاقراص , وقد تبين ان عزلات الدراسة تحمل صفة المقاومة المتعددة للمضادات (Multi drug resistance) اذ اظهرت جميع العزلات السريرية لبكتريا P.aeruginosa مقاومة بنسبة 100% للمضادات Piperacillin , Amoxicillin/Clavulanic acid , Ticarcillin/Clavulanic acid, Ceftazidime, Ceftriaxone , Aztreonam, Cefoxitin , Çefodizime, Trimethoprim/Sulphamethoxazole , Nalidixic Acid , Rifampicin , ثـم تـدرجت مــقاومة البكــتريا لـبقية المــضادات ,فــي حيـــن كانـــت حساسة لمضاد Imipenem بنسبة 100%. قدر التركيز المثبط الادنى (MIC) لعزلات بكتريا P. aeruginosa باستعمال نظام compact Vitek2 وحددت النتائج بوصف البكتريا مقاومة R او حساسة S.استعملت خمائر الخبز الجافة المستوردة من مناشئ مختلفة والمتوفرة في الاسواق المحلية من اجل الحصول على عزلات لخميرة Saccharomyces cerevisiae التي شملت كل من العزلة AY من خميرة Angel الصينية المنشا والعزلة MY من خميرة Magestic الصينية المنشا والعزلة PY من خميرة Packmaya التركية المنشا والعزلة LY من خميرة Altunsa التركية المنشا، وتم التاكد من جنس الخميرة ونوعها باعتماد المفاتيح التشخيصية التي تشمل كل من الصفات المظهرية والزرعية والفحوصات الكيموحيوية.اجريت غربلة لرواشح عزلات الخميرة من اجل انتخاب العزلة الاكفا في انتاج المواد البروتينية المثبطة باستعمال طريقة الانتشار في الحـفر لمعــرفة تاثير هــذه المواد تجاه بكتريا P. aeruginosa المنتــجة للــغشاء الحــيوي قيد الدراسة , لم تظهر الرواشح الخام (غير المركزة) لعزلات خميرة S. cerevisiae اي تاثير تثبيطي ضد العزلات البكتيرية , في حين ادى تركيز راشح خميرة AY S. cerevisiae لمرة واحدة ولمرتين الى اظهار فعاليتها التثبيطية تجاه عزلات بكتريا P. aeruginosa المنــتــجة للغشاء الحيوي وبمعدل اقطار تثبيط بلغ (15.5, 20.4) ملم عـلـى الـتـوالـي . فـي حـين بــلــغ مــعــدل اقـــطارمــنطـــقــة الــتثــبيط لراشح خميرة PY S. cerevisiaeالمركز لمرة واحدة ولمرتين (13.8, 18.2) ملم على التوالي.اظهرالراشح المركز لعزلة AY S.cerevisiae المنماة في وسط Yeast Extract Glucose Peptone Broth (YEGPB) تاثيرا تثبيطيا واضحا تجاه العزلات قياسا براشح العزلة المنماة في وسط (PDB) Potato dextrose broth . عـدت مدة الحضن (24) ساعة افضل مدة زمنية تحتاجها الخميرة لتحفيز انتاج المواد المثبطة.شـملت خــطوة تنــقية البروتينات المثبطة استعمال كبريتات الامونيوم بنسبة اشباع تراوحـت بــين 30 الــى 70% لتــرســيب الــبروتينات الــموجــودة فـــي وسط النمو للعزلة AY S. cerevisiae , وكان للبروتين المثبط المنقى جزئيا فعلا مضادا لنمو البكتريا . اذ بلغ التركيز المثبط الادنى للبروتين المثبط المنقى جزئيا (64) ملغرام/ملليلتر, ما عدا عزلة واحدة بلغت قيمة (MIC) لها (32) ملغرام/ملليلتر. وتباينت فعالية البروتين المثبط والمنقى جزئيا المضادة لتكوين الغشاء الحيوي على عزلات بكتريا P. aeruginosa , كانت العزلة الاكثر تحسسا للبروتين المنقى جزئيا العزلة Ps33 والاقل تحسسا لها Ps68.

مقارنة وراثية وبكتريولوجية بين Staphylococcus spp. وStreptococcus spp. المقاومة لمضادات الــ Macrolide والمعزولة من اصابات سريرية مختلفة == Genetic and Bacteriological Comparative between Staphylococcus spp. and Streptococcus spp. resistance to Macrolide , isolated from different clinical infections

اسم المؤلف: سيف علي محمد الحيالي
اسم المشرف: هادي رحمن رشيد الطائي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: جمعت 200 عينة من مصادرمتنوعة شملت ( الادرار، والدم ، ومسحات الاذن الوسطى ، والقشع ، والجروح ، والبلعوم ، ومسحات مهبلية ) من مستشفيات ( بعقوبة العام ، البتول للولادة ، وبلدروز العام ) بالاضافة الى بعض المراكز الصحية وذلك للفتره من 1/ 9 / 2013 لغاية 1 /1 /2014 ، حيث اظهرت 75 عينة وبنسبة (37.5%) نموا سالبا للزرع البكتيري و125 عينة (62.5%) نموا موجبا للزرع البكتيري . تم الحصول على 40 عزلة تعود للجنسين Staphylococcus spp. وStreptococcus spp . اذ شخصت العزلات باستخدام الاختبارات الزرعية , والمجهرية , والكيموحياتية فضلا عن الفحص التاكيدي للعزلات باستخدام نظام api 20 staph and strep . وصلت نسبة Staphylococcus المعزولة من الدم الى 33.3% ومن الادرار 12.6% ومن البلعوم 36% ، ومن الاذن الوسطى 13.8% ، ومن الجروح 18.7% ، اما نسبة Streptococcus المعزولة من البلعوم فكانت نسبتها 30.7% ، ومن القشع 11.7% ، ومن الجروح 6.2% . تم اختبار حساسية جميع العزلات لبعض مضادات الـــ Macrolide الشائعة الاستخدام بالاضافة الى مضاد الكلندامايسين العائد لعائلة الــ Lincosamides ، اظهرت النتائج ان مقاومة S. aureus للارثرومايسين وصلت الى نسبة 50% ، وللازيثرومايسين بنسبة 45% ، وللكلندمايسين بنسبة 25% . اما S. epidermidis فقد كانت النسب كالتالي 66.6% للارثرومايسين و44.4% للازيثرومايسين ، و11.11% للكلندامايسين . اما بالنسبة للجنس S.pyogenes فكانت نسب المقاومة كالتالي 25.5% للارثرومايسين ، و25.5% للازيثرومايسين ، و14.5% للكلندامايسين . تم تحديد التركيز المثبط الادنى لمضاد الارثرومايسين للعزلات قيد الدراسة والتي اظهرت مقاومة تجاه هذا المضاد في فحص الحساسية بطريقة الاقراص (المقاومة) حيث تراوحت قيم MIC للعزلات بين (<64 - 32) مايكروغرام / مل . تم التحري عن قابلية عزلات Staphylococcus spp . و. Streptococcus spp على انتاج بعض عوامل الضراوة وقد اظهرت النتائج ان تلك العزلات كانت منتجة لعدة انواع من الانزيمات والذيفانات التي تسهم عادة في امراضيتها ومن هذه الانزيمات انزيم البروتييز، واللايبيز ، واليوريز، والانزيم المحلل للدنا ، والستافيلوكاينيز ، والستربتوكاينيز وكذلك اظهرت هذه العزلات قدرتها على انتاج اربعة انواع من الهيمولايسين (الفا ، بيتا ، كاما ، ودلتا). كما وتم التحري عن قابلية العزلات على انتاج الطبقة اللزجة Slime layer باستخدام طريقة اكار احمر الكونغـو وقد بينت النتائج ان كلا الجنسين لها القدرة على انتاج الطبقة اللزجة فقد اظهرت النتائج ان (65% ) من عزلات بكتريا S. aureus منتجة للطبقة اللزجة بينما كانت جميع عزلات S.epidermidis وبنسبة %100 منتجة للطبقة اللزجة ، بينما اظهرت 75% من عزلات S.pyogens قدرتها على انتاج الطبقة اللزجة . اجريت عملية استخلاص للدنا الكلي البكتيري لـ ( 12 ) عزلة ثم اجري تفاعل البلمرة المتسلسل PCR لعزلات Staphylococcus spp. وStreptococcuss spp. المقاومة للـ Mcrolide وذات MIC اكثر من64 مايكروغرام/ مل والموجبة النمو على الوسط المعلم بالارثرومايسين من خلال استعمال البوادىء المتخصصة التي تستهدف التسلسل النوعي للجين erm A وmef A ، رحلت نواتج التضاعف على هلام الاكاروز بتركيز 1% ولوحظ ظهور حزمة واحدة في جميع المسارات في الهلام بالمستوى نفسه بالنسبة للجينين . اظهرت النتائج ان نسبة وجود الجين erm A في عزلات S. aureus وصل الى 80% وفي عزلات S.epidermidis 30% ، وفي عزلات S.pyogens وصلت النسبة الى 50% ، اما بالنسبة للجين mef A فكانت نسبة توافره في عزلات S. aureus 20% ، وفي عزلات S.epidermidis 40% ، وفي عزلات S.pyogens 50% . | Collected 200 samples from different clinical sources included (Urine , blood , middle ear , sputum, throat , wounds , and vaginal swabs) from hospitals (Baquba teaching hospital , AL - Batool hospital and Balad Ruz teaching hospital) in addition to some health centers for the period from 1 / 9 / 2013 until 01 / 01 / 2014. As demonstrated 75 samples 37.5% were negative growth for bacterial culture and 125 62.5% positive growth of bacterial culture . 40 isolates belonging were obtained to both genus Staphylococcus spp. and Streptococcus spp. These isolated were diagnosed by using cultural tests , microscopic, and biochemical characteristics as well as a confirmatory test for the isolates using the api 20 staph & strep system . The percentage of Staphylococcus isolated from the blood is 33.3% the urine 12.6% the throat 36% , middle ear 13.8% , and the wounds 18.7%, while the percentage of Streptococcus isolated from the throat was represented 30.7% , the sputum 11.7% , and the wonds 6.2%. Antibiotic susceptibility testing was performed on all isolates against some of the commonly used Macrolide members in addition to the clindamycin return to the lincosamides family using the disk diffusion method , the results showed that the resistance of S. aureus to erythromycin reached to 50% , for azithromycin 45% , and for clindamycin is 25% . The S. epidermidis ratios were as follows 66.6% for erythromycin 44.4% for azithromycin , and 11.11% of the clindamycin ، while the resistant of S.pyogens were as follows 12.5% of erythromycin , 12.5% of the azithromycin , and 14.5% of the clindamycin . The minimum inhibitory concentration MIC for erythomycin of isolates under study which showed resistance to the antibiotic sensitivity test in a Disk diffusion method where ranged between <33 - 64 µg / mL . investigated the ability of Staphylococcus spp. and Streptococcus spp on the production of some of the virulence factors . The results showed that these isolates were productive for several types of enzymes and toxins that contribute usually in pathogenicity. One of these enzymes , protease enzyme , lipase enzyme , urease enzyme , Dnase enzyme , Staphylokinase enzyme , and Streptokinase enzyme and also showed these isolates ability to produce Four types of Heamolysin (alpha , beta , gamma , and Delta) . It also has been investigating the susceptibility of isolates to produce a Slime layer using the Congo red agar ( CRA) ، The results showed that both genus have the ability to produce slime layer and Biofilm formation , the results showed that 65% of the S. aureus producing Slime layer while all S.epidermidis isolates produced the slime layer , while showed 75% of the isolates S.pyogens ability to produce Slime layer. Conducted the process of total DNA extraction to 12 isolates under study that resist Macrolide members . polymerase chain reaction (PCR) was done for isolates of the bacterium Staphylococcus and S.pyogens resistance of Mcrolide and with MIC more than 64 micrograms / ml by using specialized primers targeting sequence specific gene erm A and mef A , deported outputs doubling the agarose gel concentration 1% and observed the emergence of a single package in all the tracks in the gel at the same level for the genes . The results showed that the percent of the presence of erm A gene in isolates of S. aureus arrived 80% and in isolates S.epidermidis 30% , and in isolates S.pyogens got to 50% , while for mef A gene the presence in isolates of S. aureus was 20% , in isolates S.epidermidis 40% , and in isolates S.pyogens 50% .

تقدير البعد الوراثي لبعض اصناف نخيل التمر Phoenix dactylifera L. المزروعة في العراق باستعمال تقانة RAPD == Estimation of the Genetic Distance for Some Date Palm Varieties ( Phoenix dactylifera L.) Grown in Iraq using a RAPD Technique

اسم المؤلف: مهند رشيد حسين الزيدي
اسم المشرف: شذى عايد يوسف
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: This study was conducted at Molecular Biology lab, Genetic Engineering Department, Agricultural Research Directorate, Ministry of Science & Technology to estimate fingerprint and genetic relationship among 9 Iraqi varieties of date palm Phoenix dactylifera L. namely Asabee AL - aroose, Zahdi, Sukari, Saada, Ashrasy, Barben, Shetwi, Khistawi and Nhair Al Saly. 20 primers were used to PCR amplify characteristic Random Amplified Polymorphic DNA (RAPD) marker fragments. Total genomic DNA was isolated from varieties fresh leaves, the average yields of DNA were in the range of 100 - 522.5 ng/ul with purity ranged between 1.66 to 1.92. Polymerase Chain Reaction (PCR) experiments were performed to detect DNA polymorphisms amplified fragments in each cultivar (their numbers and molecular weight sizes) on agarose gel electrophoresis stained by ethidium bromide. Results of RAPD experiments revealed differences in band numbers and molecular weight sizes, all tested primers yielded 157 polymorphic bands and the lowest polymorphic band numbers (5 bands) were with primers OPA - 01 and OPA - 20 while the largest number of polymorphic (12 bands) were with primers OPA - 08 and OPH - 09. RAPD analysis showed that many primers gave unique band in all samples made possible fingerprint identification of each cultivar, Ashrasy variety gave highest 6 unique polymorphic bands with primers OPA - 08, OPC - 04, OPF - 05 and OPF - 12, while Zahdy variety gave 1 unique polymorphic band (385 pb) with primer OPB - 05. Data was analyzed by using statistical program SPSS to find similarity and genetic relationship among cultivars, similarity index ranged between 0.291 and 0.511 and the biggest percentage of similarity was between Saada and Shetwi. Dendogram analysis has separated varieties into two main groups, the first main group consists of three sub - cluster or sub - group, the first sub - cluster contained Saada and Shetwi varieties while the second sub - cluster included Zahdi, Ashrasy and Nhair Al Saly and the third sub - cluster consisted Asabee Aroose cultivar. The second main group was divided into two sub - group, the first sub - group consisted of Berben and Khistawi while the second sub - group included Sukary.

تاثير الرش بالبرولين والارجنين في نمو وحاصل الباذنجان في الزراعة المحمية == Effect of Spraying Proline and Arginine in the Growth and Yield of Egg Plant in Protected culture

اسم المؤلف: زينب نبيل ابراهيم
اسم المشرف: نجم عبد الله جمعة الزبيدي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:

تاثير مواعيد الزراعة والاصناف في نمو وحاصل زهرة الشمس (Helianthus annuus L.) == The effect of difference genotype and planting date in growth and production of (Helianthus annuus L .

اسم المؤلف: هديل احمد مهدي الساعدي
اسم المشرف: نجم عبد الله جمعة الزبيدي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: نفذت التجربة في مشتل مديرية زراعة ديالى - بلدروز في الموسم الربيعي لعام ( 2014 ) لدراسة تاثير اربعة مواعيد زراعة 1/2 و15/2 و1/3 و15/3 على صفات النمو والحاصل ومكوناته والصفات النوعية لثلاثة اصناف من نبات زهرة الشمس الصنف فلامي , يوروفلور , زهرة العراق . تم استعمال القطع المنشقة Split Plot بتصميم القطاعات العشوائية الكاملة R.C.B.D بثلاثة مكررات حيث تضمنت مواعيد الزراعة المعاملات الرئيسية الاصناف المعاملات الثانوية ويمكن تلخيص النتائج كالاتي : - 1 - اظهرت مواعيد الزراعة تاثيرات معنوية في صفات النمو والحاصل والنوعية . اذ تفوق الموعدين الثاني والثالث في جميع صفات النمو كما تفوق الموعد الثاني في جميع مكونات الحاصل ونسبة الزيت وحاصله.2 - اظهرت الاصناف اختلافات معنوية فيما بينها في بعض صفات النمو ومكونات الحاصل والنوعية . اذ تفوق الصنف زهرة العراق فــــي المساحة الورقية وعدد الاوراق / نبات . وتفوق الصنفين زهرة العراق يوروفلور في النسبة المئوية للاخصاب ، وتفوق الصنفين يوروفلور وفلامي في قطر الساق تفوق الصنفين زهرة العراق ويوروفلور في نسبة الاخصاب المئوية وتفوق الصنفين يوروفلور وفلامي في وزن 1000 بذرة وحاصل النبات ونسبة وحاصل الزيت .3 - كان تاثير تداخل مواعيد الزراعة والاصناف معنويا في بعض صفات النمو والحاصل والنوعية ، حيث اعطى الصنف زهرة العراق في الموعد الثاني اعلى متوسط في ارتفاع النبات . واعطى اعلى متوسط في ارتفاع النبات والمساحة الورقية ودليل الكلوروفيل . واعطى الصنف يوروفلور اعلى متوسط في الوزن الرطب والجاف في القرص الزهري وقطر الساق وقطر القرص والنسبة المئوية للاخصاب وعدد البذور ووزن 1000 بذرة وحاصل النبات وحاصل البذور والنسبة المئوية للزيت وحاصل الزيت | This experiment was conducted in Baladrus plant nursery in spring (2014) to stady effect of four sowing dates ( 1/2 , 15/2 , 1/3 and 15/3 ) on plant yield and its component and the second factor was sunflower varieties ( Flamy , Eourflor , sun flower ) in a split plot desing in R.C.B.D with three repilication the sawing date have the main plots varieties in the sub - plots and the result summarized in : 1 - the sowing date show a signifieaht effects in some of growth yied and quality characters . the second and third date exceeded the others in plant height leaves number in plant but the second dates exceeded others in fertilization percentage and leaves seed number in disc .2 - varieties show significant differences in growth yield and its components and quality sun flower exceeded others in leave area . but Eourflor and Flamy gave increase in stem diameter . sunflower and Eourflor gave in higer fertilization percentage Eourflor and sunflower exceeded others in 1000 seed weight plant yield and Oil yield .3 - Interaction between sowing dates with varieties gave signnificantly result in growth yield and yield quality sunflower gave high means in the second and third dates in plant height and leave area and chrorophyll index in the second date Flamy gave the highest means in leave area in the third date Eourflor gave the highest means in stem diameret wet and dry weight for the flower disc in the second date Eouroflor and Flamy exceeded others in all of yield components yield and Oil percentage in the second date .

تركيز بعض ملوثات الهواء في بعض المناطق الصناعية والحضرية من محافظة البصرة العراق == Concentration of some air pollutant in some industrial and urban areas from the province of Basrah - Iraq

اسم المؤلف: معتز حكمت عثمان
اسم المشرف: نايف محسن عزيز
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: The current study was conducted, from October 2013 to September 2014, aimed to identify the temporal and spatial variations of air pollutants in stationary sites included Basrah oil refinery and southern gas company in addition to sources included intersections traffic in Basrah province (Alkzizah, Tigari and Jazaier streets).The results showed that in Basrah oil refinery the CO and SO2 concentration were less than the national and World Health Organization standards, while NOX were higher than the national and global standards reached 1ppm.The second site, which is southern gas company was as follows : CO gas was less than the national and global standards as well as NOX, while SO 2 was higher than the national and global standards reached 0.63 ppm.For intersections traffic sites (intersections)the results was as follows : CO was less than the national and global standards, while NOX was higher than the national and global standards reached 2.75ppm as well as SO2 at all intersections reached 0.65 ppm.Ambient air quality was recorded through the seasons of the year using the accurate measurement devices in various study sites of the province of Basrah. The results were indicated that the concentration of all the gases rate was higher in the winter than those recorded in summer due to the difference in climatic conditions, especially wind speed and direction, air temperature and moisture.The results showed a temporal and spatial variations in concentrations of studied air pollutants in ambient air interchanges traffic roads within the province of Basrah centerdue to difference in the rush hours , the activities of the various rights in traveling or shopping as well as the routine jobs procedure of the official working hours.The study showed variation in the concentrations of total suspended particles and black carbon among studied traffic intersection recorded highest concentration of black carbon at Quzaizah amounted to 27 μg/ m3 As the TSP recorded less concentrations 392 μg/ m3. But at the intersection of Tijari Street has concentrations of black carbon amounted to 11.3 μg/ m3 ,As for particles recorded 412 μg/ m3, either at the intersection of Jazair Street was recorded black carbon of 16.2 μg/ m3, As for particles amounted to 420 μg/ m3.All intersections have exceeded the global limitations of particles amounted of 150 μg/ m3 and local of 350 μg/ m3It can be concluded from the current study that the province of Basrah are polluted by air pollutant NOX gas in Basrah oil refinery and SO2 in the site Southern gas company NOX and SO2 at all intersections traffic sites located within the current study.

دراسة تصنيفية وبيئية لحشرات نصفية الاجنحة المائية وشبة المائية Heteroptera : Hemiptera في محافظة البصرة == Taxonomical And Ecological Study of Aquatic And Semiaquatic Insects(Heteroptera : Hemiptera) In Basrah Provience

اسم المؤلف: اسماء عبد الزهرة سبع العيداني
اسم المشرف: ضياء خليف كريم
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: علوم الحياة
الدرجة: دكتوراه
الجامعة: جامعة البصرة
اللغة: العربية
مكان الجامعة: البصرة
الصفحات الاولى:
المستخلص: Present studies dealing with the taxonomical and ecological studies of the aquatic Hemiptera : Heteroptera in eight stations in Basrah provience ,which are : Al - Medina , Al - Qurna , Basrah center ,Shat Al - Arab , Al - Zubair , Abu Al - Khasib , Al - Faw and Sullein Marsh, collection was done twise monthly during the period from April 2012 to March 2013 .Results showed the presence of nine species belonging to aquatic Hemiptera , three belonging the group Gerromorpha and six the group Nepomorpha,as follows : Group : Gerromorpha(Popov,1971)Family : Gerridae ( Leach , 1815)1 - Aquarius nebularis(Schellenberg,1800)Family : Mesoveliidae(Douglast and Scott , 1867 )2 - Mesovelia vettigera( Horvath , 1895 ) ( apterous )M .vettigera( Horvath , 1895 ) ( macropterous )Family : Macroveliidae3 - Macrovelia hornii(Uhler , 1872 )Grouop : Nepomorpha(Popov,1968)Family : Nepidae ( Latreille , 1802 )4 - Nepa cinerea( Linnaeus , 1758 )Family : Corixidae ( Leach,1815 )Subfamily : Cymatianiae ( Walton , 1940 )5 - Cymatia bonsdorffii(Sahlberg , 1819 )Subfamily : Corixinae ( Leach , 1815 )6 - Sigara(Vermicorixa) latelaris ( Leach , 1815 )Family : Belostomatidae ( Leach , 1815 )7 - Lethocerus fakir(Mayr , 1852)Family : Notonectidae ( Latreille , 1802 )8 - Anisops sardeus(Herrich - Schaeffer , 1849)AbstractBFamily : Pleidae ( Fieber , 1851 )9 - Plea leachi(McGregor and Kirdaldy,1899)Among the collected species ,six were regarded as new records to the Iraqi fauna of this order,they were : A. nebularis,M. vettigera( macrpterous) , M . vettigera( apterous), Ma.hornii , N.cinerea , C.bonsdorffii andP.leachi.Study also deal with the measuring of some ecological aspects like air and water temperatures , salinity , pH and dissolved oxygen ,and their effected on the monthly distribution of the aquatic Hemiptera. Temperature was the most effective aspect in the monthly distribution of the insects ,as the lowest numbers were recorded in Summer months and the highest number were in Winter months , the ranges of the air temperatures were 9 - 43º C and water temperatures were 2 - 33º C .The highest concentrations of salinity was recorded in Al - Zubair , Abu Al - Khasib and Al - Faw reached to 35g/l in Al - Zubair in July , the pH ranges were the same in all stations and was alkalid , their ranges were 7.3 - 7.8 , about the dissolved oxygen it was low and same in all stations except in Sullein Marsh which reached to 11 mg/l in January .The concentration of some heavy metals in water also studied like Fe, Pb, Cu and Zn , the highest concentrations were to Fe and Zn in Basrah center and Al - Zubair, and the highest concentration for Fe in Al - Zubair reached to 6362.21μ/L in August, while highest concentration for Zn in Basrah center reached to 1365.92μ/L in July, while in the other stations the concentrations were lowest .The study also used the water boatmen Sigara latelaris(Leach,1817) as bioindicators of heavy metals pollution in the water of Basrah provience , and taken Sullein marsh as control , and measuring the concentrations of the heavy metals in insects tissues , water and sediments , the results showed that the insects collected from the water of the center of the city can accumulate Fe andAbstractCZn in their tissues and the highest concentrations reached to 3562.16 and 3778.12 μ /gm for Fe and Zn in July , while in the insects taken from Sullein marsh were 286.25 and 285.17 μ /gm .The concentrations of heavy metals in water and sediments of the city center were measured and the highest concentration was for Fe and Zn , and reached to 1569.91 and 1365.91 μ /L of the water of city center , while reached to 215.01 and 223.14 from the same metals in the water of Sullein marsh , the concentrations of the same metals were recorded in sediments of the city center and reached to 2865.16 and 1982.56 μ /gm for Fe and Zn , and the highest concentrations of the same metals in Sullein marsh were 418.57 and 393.74 μ /gm .

دراسة نوعية للبكتريا السالبة لصبغة كرام المعزولة من بيئة واخماج مستشفى عفك العام == Qualitative Study of Gram Negative Bacteria Isolated from Environment and Infections of Afak General Hospital

اسم المؤلف: زينب عبد الكريم عليوي الفرحاني
اسم المشرف: علي عبد الرحيم الناشي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: شملت الدراسة جمع 400 عينة سريرية وبيئية من مستشفى عفك العام في محافظة الديوانية للفترة من 1/11/2012 ولغاية 31 /3/2013 لغرض دراسة نوعية البكتريا السالبة لصبغة كرام والملوثة لبيئة واخماج المستشفى ومدى حساسية العزلات البكتيرية ومقاومتها للمضادات الحيوية والمطهرات شائعة الاستخدام .اظهرت هذه الدراسة ان 236 عينة وبنسبة 59% قد اعطت نتيجة موجبة للفحص البكتيري للبكتريا السالبة لصبغة كرام توزعت بين 31 عينة من مصادر سريرية وبنسبة 56.37 % و206 عينة من مصادر بيئية وبنسبة 59.43% بينما كانت 164 عينة وبنسبة 41% سالبة للفحص البكتيري للبكتريا السالبة لصبغة كرام توزعت بين 24 عينة من مصادر سريرية وبنسبة 43.63% و140 عينة وبنسبة 40.57 % من مصادر بيئية من مستشفى عفك العام .كانت العزلات البكتيرية السالبة لصبغة كرام المشخصة والبالغة 236 عزلة قد توزعت على اربعة اجناس وهي Pseudomonas شملت نوعين : P.aeruginosa 12.7% ، P. flourescens 8.5 % ، Klebsiella شملت K. pneumoniae 10.5% ، K.oxytoca 10.5% ، Serratia marcescens 3.4% ، Acenitobacterشملت A. baumanni 2.1% ، A.lwoffii 1.2% ، A.calcoceticus 0.8 %، Escherichia شملت E. coli 45.5% ، E.hermanni 4 % . كانت نسبة التلوث البكتيري في العينات السريرية الحروق والجروح قد بلغت 53.3 ، 60 % على التوالي ، بينما التلوث البكتيري في العينات البيئية المتمثلة بردهات دخول المرضى ، المطبخ ، صالات العمليات الجراحية ، ردهات الخدج وملابس العاملين في المستشفى قد شكلت النسب 66.6 ، 53.8 ، 60 ، 54.6 ، 40% على التوالي . كما اختبرت حساسية العزلات البكتيرية السالبة لصبغة كرام اتجاه 13 مضادا حيويا وقد اظهرت العزلات البكتيرية مقاومة وحساسية مختلفة تباينت حسب نوع العزلات البكتيرية وطبيعة المضاد الحيوي .كانت اعلى مقاومة اظهرتها بكتريا K.pneumoniae تليها K.oxytoca اتجاه مضاد Tetracycline اذ بلغت النسبة 96% و92% على التوالي . كما تميزت كل من بكتريا E.hermanni ، A.baumanni بمقاومتة عالية اتجاه ال Gentamycin اذ بلغت 80.3% ، 80% على التوالي اما P.aeruginosa فقاومت ال Tobramycin بنسبة عالية بلغت 90 %، بينما تميزت جميع البكتريا المعزولة في هذه الدراسة بحساسيتها اتجاه مضاد ال Imipenem . اما اختبار حساسية العزلات البكتيرية اتجاه المطهرات فقد ابدت مقاومة متفاوتة حسب نوع العزلات البكتيرية وتركيز وطبيعة المطهر وشملت المطهرات المستخدمة في التضاد الجرثومي كلا من الفورمالين والبوفيدين - ايودين والهبتين والديتول حيث كانت اعلى مقاومة اظهرتها E.coli اتجاه مطهر الفورمالين بتركيز 12.5% اذ بلغت 93.4% . درست بعض عوامل الضراوة المنتجة من الانواع البكتيرية ومنها انتاج المحفظة فوجد ان عزلات كل من بكتريا A. baumanni، P. aeruginosa ، K. pneumoniae وE.coli منتجة للمحفظة وبنسبة 40 ، 33.33 ، 100 ، 23.36 % على التوالي ، اما انزيم الهيمولايسين فوجد ان جميع عزلات A.baumanni غير منتجة لهذا الانزيم في حين جميع عزلات P. aeruginosa كانت منتجة لهذا الانزيم بنسبة 100% ، اما انزيم البروتيز فوجد اعلى نسبة لانتاجه في P.aeruginosa بنسبة 100 % وادنى نسبة في بكتريا E.coli وبنسبة 14 % ، كانت اعلى نسبة لانتاج انزيم الجلاتينيز من قبل كل من A.baumanni وP.aeruginosa وبنسبة 100 % وتميزت عزلات K.pneumoniae بعدم القدرة على انتاجه . | The study included the collection of 400 clinical specimens and environmental from Hospital Afak General in the province of Diwaniyah for the period from 1/11/2012 until 3/31/2013 for the purpose of study quality the gram negative bacteria and contaminated environment and hospital infections and the sensitivity of the bacterial isolates and their resistance to antibiotics and antiseptics commonly used . This study showed that 236 sample and 59% gave a positive result for the examination of bacterial for bacteria gram negative distributed among the 31 sample sources of clinical and increased by 56.37% and 206 samples from environmental sources and increased by 59.43% while the 164 sample and 41% negative for screening bacterial for bacteria gram negative distributed among the 24 samples from clinical sources, and by 43.63% and 140 sample and by 40.57% from environmental sources of Afak General Hospital. The bacteria gram negative isolates diagnosed, amounting to 236 isolation has distributed over four genera Pseudomonas They included two types : P.aeruginosa 12.7%, P. flourescens 8.5%, Klebsiella included K. pneumoniae 10.5%, K.oxytoca 10.5%, Serratia marcescens 3.4% , Acenitobacter included A. baumanni 2.1%, A.lwoffii 1.2%, A.calcoceticus 0.8%, Escherichia included E. coli 45.5%, E.hermanni 4%. The percentage of bacterial contamination in clinical samples of burns and wounds had reached 53.3,0.60% respectively, while bacterial contamination in environmental samples of lobbies entry of patients, the kitchen, Eat surgeries, lobbies premature and clothes of hospital personnel had formed ratios of 66.6, 53.8 0.60 , 54.6 0.40%, respectively. The sensitivity of the bacteria gram negative isolates tested for direction 13 have shown antibiotic - resistant bacterial isolates and different sensitivity varied by type of bacterial isolates and the nature of the antibiotic. The highest resistance exhibited by the bacteria K.pneumoniae followed K.oxytoca direction Anti Tetracycline as the percentage reached 96% and 92%, respectively. It also marked by both bacteria E.hermanni, A.baumanni resisting the trend of high Gentamycin reaching 80.3%, 80%, respectively, and P.aeruginosa resisting the Tobramycin high proportion reached 90%, while all characterized bacteria isolated in this study sensitivity counter direction the Imipenem . The test of the sensitivity of the bacterial isolates direction disinfectants have shown resistance varying according to the type of bacterial isolates and the concentration and nature of the cleanser included disinfectants used in contrast bacterial both Formalin and Povidin - Iodine and Hibtin and Dettol had the highest resistance exhibited by E.coli direction antiseptic Formalin concentration of 12.5% reaching 93.4% . I studied some of the virulence factors produced by bacterial species, including the production of the wallet and found that all isolates of bacteria A. baumanni, P. aeruginosa, K. pneumoniae and E.coli producing capsule and by 40, 33.33, 100, 23.36%, respectively, while the enzyme was found haemolysin that all isolates A.baumanni unproductive for this enzyme, while all P. aeruginosa isolates were producing this enzyme by 100%, while the enzyme protease and found a higher proportion of production in P.aeruginosa 100% and the lowest percentage in the bacteria E.coli and 14%, the highest percentage for the production of an enzyme gelatinase by both A.baumanni and P.aeruginosa and 100% K.pneumonae isolates were characterized by the inability to produce it

فحص عوامل عنق الرحم باستخدام فحصي اختراق النطف واتصال النطف بمخاط عنق الرحم للازواج العقيمين == Examination The Cervical Factors By Using Sperm Penetration Test And Sperm Cervical Mucus Contact Test In Infertile Couples

اسم المؤلف: الاء عمران مطلك الحسناوي
اسم المشرف: فارس ناجي عبود الهادي | بشرى جابر الربيعي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: The present study was conducted in Infertility unit / Babylon Hospital of Gynecology and Children in a period 21/1/2013 to 15/1/2014. The study involved communiqués tests for males and females complaining from unexplained infertility , in which seminal fluid analysis were normospermia. The study include 45 seminal fluid specimens used in connecting test (SCMCT) and penetrating test (SCMPT) with the specimens of cervical mucus of their wives. Cervical mucus aspirating in day 12 - 14 of menstrual cycle. The study aimed to evaluate the husband's sperm activity and concentration in vitro with cervical mucus. Seminal fluid analysis were achieved and then semen specimens used to performed two in vitro tests : SCMCT and SCMPT. sperm parameters ( sperm concentration and sperm motility grade a, b, c, and d) were evaluated in each mentioned test and compared the results with the values of sperm parameters in seminal fluid analysis (control). The statistic results showed 60% of the study specimens of SCMCT was negative and 40% positive, while in percentage of positive results was 33% and 67% negative in penetrating test. The results revealed a significant (p<0.001) decrease of sperm concentration and grade a and b sperm motility, a significant (p < 0.001) increase of sperm motility grade c and immotile sperm grade d in connecting test with cervical mucus compared to control. The results of penetrating test showed significant (P< 0.001) decrease of sperm concentration in the brim, medium and distal region of cervical decrease (p<0.001) of sperm concentration in both medium and distal region compared to brim region, and a significant (p < 0. 001) decrease in distal region compared to medium region ; A significant (P < 0.001) decrease of grade a + b motility percent in all three regions of cervical mucus area compared to control, while no significant differences (P > 0. 05) between brim, medium and distal region of cervical area ; Only grade C sperm motility in distal region showed a significant decrease (P < 0. 001) compared to control and both brim and medium regions. The results revealed significant increase of grade d (P<0.001) in brim and medium region (P < 0.01) compared to control, while there was a significant decrease (P< 0. 001) in distal region compared to control, brim and medium regions. The correlation (r) study of sperm concentration and sperm motility ( grade : a + b, c and d) with age and infertility period in SCMCT showed non significant (P > 0. 05) correlation, except the correlation between a + b grade sperm motility and age it was noticed a significant negative correlation (P < 0. 05) and positive correlation(p> 0.05) between grade c sperm motility and age. The study of linear correlation of sperm parameters in penetrating test showed a significant(P<0.05) negative correlation between grade a+b sperm motility and age in brim and medium region of cervical mucus area. Except this, there are no significant correlation (P>0.05) between sperm concentration and sperm motility for all grades (a + b, c and d) with age and infertility period in both brim and medium regions It was concluded that both test : SCMCT and SCMPT were necessary to diagnosis the causes of infertility when there were normal finding of both seminal fluid analysis and female criteria. mucus area compared to sperm concentration in control, also a significant

تقييم دور الجين في دم نساء عراقيات مصابات بسرطان الثدي == Evaluation The Rule of Mammoglobin A Gene In Blood of Iraqi Women With Breast Cancer

اسم المؤلف: نانسي فيصل رشيد
اسم المشرف: امنة نعمة الثويني
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Mammaglobin A is a member of the superfamily of secretoglobins. Its expression is highly specific for mammary tissue and has been shown to be overexpressed in breast tumor tissue, indicating that mammaglobin A might confer a growth advantage to mammaglobin A - expressing tumor cells. The present study was undertaken to develop the mammaglobin A as a serum biomarker for the early detection of breast cancer, and breast cancer patients follow up after treatments. This study had taken ten months, it involved 84 patients with age ranged 15 - 74 years who had diagnosed with either stage I to III breast cancer or benign lesions of breast at certain Iraqi hospitals and breast cancer centers, and a total number of 10 apparently healthy individuals, with comparable age range of patients as control group.Ten ml of venous blood was collected from studied groups in order to be used for measuring estrogen and progesterone levels, Reverse transcription polymerase chain reaction (RT - PCR) test for detection of their serum m - RNA mammaglobin A existence, and serum mammaglobin A levels using Enzyme - Linked ImmunoSorbent Assay (ELISA) technique. Demographic study revealed that most cases enrolled in this study were women within their reproductive age 25 - 44 years old. Distribution of patients according to age group, marital status, and lactation status showed no statistically significant association (P>0.05) with any of these variables, in all comparison.The hormonal study states that elevated levels of estrogen were observed in 52 (62%) patients, also decrease in progesterone levels were observed in 50 (59%) patients out of the 84 cases studied. This indicate a highly significant SUMMARYassociation (p < 0.01) between breast cancer risk, elevated estrogen levels, and decrease in progesterone levels. Mammaglobin A protein was detectable by ELISA in 100% of breast cancer patients (65 case), and not in any benign tumor patients (19 case) nor healthycontrol women (10 females) which indicate the highly specificity of ELISA test (P<0.01) for measuring mammaglobin A levels in patients serum. With high concentration of protein in sera of women with late stage of breastcancer compared with a low protein concentration in sera of women with early stage of breast cancer. By applying a statistical analysis on the result it was found that serum mammaglobin A ELISA test is highly significant (P<0.01) for, differentiation between patients with metastatic breast cancer and patients with early diagnosed breast cancer. In this work, SCGB2A2 gene which coded for mammaglobin A was detected in 64 out of 65 breast cancer cases, but not in the benign or healthy individuals indicating its high specificity as a marker gene (SCGB2A2) for cells derived from mammary glands. A statistical analysis was made for comparison between the three groups, E - Malignant Tumors \ Early Stage, L - Malignant Tumors \ Late Stage, and B - benign, (E, L, and B) which show that the results of molecular study of mammaglobin A detected by RT - PCR is statistically high significance (P<0.001) when used for the differentiation between E group and B group, also between L group and B group. The results showed that mammaglobin A can become an important tool for detecting primary and metastatic breast cancer, and predicting disease outcome.

دراسة تشخيصية جزيئية ودمية وكيموحيوية لانواع الاكريات Eimeria spp. في الدجاج المصاب طبيعيا وتجريبيا في محافظة الديوانية == Molecular Diagnosis Study And Haematological And Biochemical of Coccidiosis Eimeria Spp. In Natural And Experimental Infection Chicken In Al - Diwanyia City

اسم المؤلف: خالد ثامر مطر الشيباني
اسم المشرف: خيري عبد الله داود العكيلي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة في البيت الحيواني التابع لقسم علوم الحياة / كلية التربية / جامعة القادسية للمدة من 1/9/2013 ولغاية 1/9/2014، للتحري عن انواع طفيلي الاكريات المتطفلة على الدجاج المصاب طبيعيا وعزل الطفيلي من الحالات المصابة الواردة الى المسستشفى البيطري | The study conducted in The animal house of the Biology department, College of Education, University of Al - Qadisiyah during the period from 1/9/2013 to 1/9/2014. The study diagnosed the species of Eimeria parasites in naturally infected chickens in poultry farms. Eimeria oocysts was isolated from the infected chickens feaces in Veterinary hospital and Veterinary clinics.Then, the cases diagnosed initially in the laboratory by direct examining stool smears and the positive cases were recorded.Through this study, oocysts were isolated, and two species of Eimeria parasites were diagnosed (E.tenella and E.maxima) by using the technique of Conventional Polymerase Chain Reaction technique.There are 186 cases of infected chicken which examined, and the results showed that (186 out of 315)stool Samples(59%), include infected with two species of Eimeria parasites E.tenella was in (135 out of 186, 72.5%) while the species E.maxima was in(51 out of 186, 27.4%). The experimental study was conducting two experiments were done.The first experiment, depend on the experimental infection of E.tenella species which was used for two types of chicken, (Ross type which is foreign, and local breed).The second experiment, includes experimental infection with the species E.maxima also in two types of chickens that mention above. Symptoms and clinical signs of the first experiment on foreign chickens included were recorded it is noticed that infected the chickens with E.tenella have was dull, unorganized feather and loss of appetite in addition to bloody diahrreha, loss weight, emaciated and isolated. The Gross Lesions include congestion of cecum and an increase in their sizes while inside the cecum, many ulcerated lesions were noticed, filling cecum with mucus and blood, liver was congested and enlarged. Microscopicale examination recorded congestion was full with blood cells and necrotic tissues cecum and falling of cells in the cecum lumen. Many different stages of the parasites were noticed as schizonts, merozoites, gametes and oocysts Hematological parameters showed high significant decrease (p?0.05) in Hb (0.38±5.94 mg / 100ml) in the fourth week of infection, PCV (0.68±18.47%) in the fifth week and RBC was (1.58±0.02x106) in the fourth week. Also, the infection led to significant increase difference of WBC (30.19±0.95x103) in the seventh week of infection in comparison of uninfected chickens of the control sample. The biochemical parameters of blood serum showed that significant decrease (p?0.05) in glucose concentration (156.98±1.75mg / 100ml in the fourth week of infection), cholesterol concentration and total protein concentration (62.11±4.40, 1.92±0.92 mg / 100ml respectively in the fifth week of infection).Also, Uric acid increased significantly (p?0.05) to reach(10.19±0.52mg / 100ml) in the fourth week of infection in comparison of uninfected chickens of the control group. Average weight was showed the significant decrease (p?0.05) of the chickens quantity (368.88±3.01gr in the fourth week of the infection), and an increase the percentage of mortality(23.33±0.23% in the fifth week of infection), addition to the decrease of consuming food by bird (282.8±2.19gr in the fifth week of the infection) in comparison of uninfected chickens of the control group. While the results of local breed chickens in the first experiment were less severity than that with the foreign type (Ross) but the colour of the stool was brown mixed with blood. The Gross Lesions showed pathological changes in the cecum as congestion and bleeding. Moreover, ulcers areas were smaller and less in number in comparison with the infected foreign chickens by the same parasite. The microscopic pathological changes of E.tenella in the cecum showed hyperplasia in goblet cells and congestion of blood capillaries with less number of stages of the parasite in comparison with the foreign chickens. The hematological parameters showed significant decrease (p?0.05) in Hb concentration and percentage of PCV (7.13±0.31mg/100ml, 21.26±1.82% respectively in the fifth week of infection), while the RBC count was (2.05±0.12 x 106 in the fourth week of infection and significant increased (p?0.05) in WBC count was (30.63±0.38 x 106 the sixth week of infection) in comparison with the chickens of the control group. The biochemical parameters of blood serum showed the significant decrease (p?0.05) in glucose concentration (169.60±2.16mg/100ml in the fifth week of infection), cholesterol concentration and total protein concentration (70.89±2.01, 2.25±0.39mg/100ml respectively in the fourth week of infection). Uric acid increased significantly (p?0.05) to reach (7.62±0.90mg/100ml) in the fifth week of infection in comparison with the control group. Average of the weight was significant decrease (p?0.05) (190.28±3.81gm), high increase in the average of mortality (10±0.20%), and high decrease in the consuming food average (413.4±3.91gr) in the fourth week of infection in comparison with the uninfected control group. The second experiment of the foreign chicken which infected experimentally by E.maxima showed asymptoms and clinical signs were nearly similar in the foreign chickens that infected with E.tenella, but mild in severity is average. The symptoms include fatigue, fallen wings, loss of appetite, mucus diarhea mixed with blood. The Gross Lesions showed severe changes in the infected part of intestine as congestion and filling of intestine with mucous secretion of bad odour. While the microscopic pathological changes showed the tissues section of intestine were damaged, necrosis of intestine villi due to the large number of different stages of the parasite with hyperplasia of goblet cells. The hematological parameters showed significanty decrease (p?0.05) in Hb concentration, percentage of PCV and RBC count (7.55±0.61mg/100ml, 23.62±0.81% and 2.08±0.10x106 in the fourth week of infection) and significant increased (p?0.05)in WBC count (31.26±0.63x106 in the seventh week of infection) in comparison with the uninfection control group. The biochemical parameters of blood serum showed that there was the significant decrease (p?0.05) in glucose concentration (164.86±2.11 mg /100ml in the fifth week of infection), cholestrol concentration and total protein (77.14± 3.38, 2.24±0.36 mg/100ml) and significant increased (p?0.05) in Uric acid concentration (8.20±0.79mg/100ml) in the fourth week of infection in comparison to the control group. The results of productive characteristics refered to significant decrease(p?0.05) in the weight of chickens (479.28±0.23gr) and significant increase (p?0.05) in mortality (10±0.11%) during the fifth week of infection. Also, there was significant decrease (p?0.05) in the consumed food by birds (368.5±2.10gr) during the fourth week of infection in comparison to the chicken of the control group. While the results of local breed chicken in the second experiment, the symptoms and clinical signs showed no sign of disease, and the chickens were active in spite of presence of infection and the releasing of Oocysts. The Gross Lesions showed the inflammation and congestion, onlargment of the infected part of small intestine, little mucous and liquid inside the lumen.As far as microscopic changes are concerned, the tissue examination showed some necrosis and damage of the villi ends and an increase in the size of the goblet cells. Hematological parameters showed to the significant decrease (p?0.05) in Hb concentration and percentage of PCV (7.86±0.45 mg/100ml, 23.60±0.53% respectively during the fifth week of infection), RBC count (2.10±0.13 x 106 in the fourth week of infection) and significant increased (p?0.05) in WBC count (32.98±0.58 x 106 during the sixth week of infection) in comparison with the control group. The biochemical parameters of blood serum showed that there was the significant decrease (p?0.05)in glucose concentration and cholestrol concentration (170.60 ±2.01, 79.70±2.69 mg/100ml respectively in the fourth week of infection), total protein concentration (2.42±0.31mg/100ml in the fifth week of infection) and significant increased (p?0.05) in Uric acid concentration (6.52±0.82mg/100ml in the fourth week of infection) in comparison to the uninfection control group. Average of the weight referred to significant decrease (p?0.05) of chickens (213.16±3.12 gr) and consuming food (481±3.21 gr) during the fourth week of infection.While there were no differnces in the percentage of mortality comparison with the uninfected chicken of the control group. Also, the results of the number of released oocysts showed significant increase (p?0.05)in number of released oocysts with stool by infected foreign ckicken(Ross) in comparison with the local breed chicken which infected by the same parasite.Also, the number of the dropped oocysts of E.tenella were higher than that of E.maxima.

دراســــة الفعالية التضادية لجرثومة Bifidobacterium spp تجاه جرثومة Staphylococcus aureus المقاومة للمثيسلين == Studying The Antagonistic Activity of Bacteria Bifidobacterium Spp Against Bacteria Methicillin Resistance Staphylococcus Aureus

اسم المؤلف: انغام نجاح هادي الخفاجي
اسم المشرف: سهام جاسم الكعبي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة التحري عن الفعالية التضادية لجرثومة Bifidobacterium spp تجاه عزلات سريرية لجرثومة Staphylococcus aureus المقاومة للمثيسلين، ودراسة تاثير تلك المواد على عوامل الضراوة المنتجة من قبل S.aureus المقاومة للمثيسلين مثل الغشاء الحيوي Biofilm وا | This study included the investigation of Bifidobacterium spp antagonistic activity against Staphylococcus aureus isolates, and the effect of these antimicrobial agent on virulence factors produce by MRSA (Methicillin Resistance Staphylococcus aureus) such as Biofilm and Slime layer. One handered different samples were collected to isolate Bifidobacterium spp and 50 clinical isolates of Staphylococcus aureus from clinical specimens in AL - Najaf AL - Asharaf governorate during the period of (10/10/2013 - 20/1/2014) by the cultural characteristic colonies, microscopic for cells, biochemical tests and polymerase chain reaction PCR technique to detect 16SrDNA, the results showed that 13 isolates belong to Bifidobacterium spp and there are divergent gene content between these isolates and they have been bearing lm26/ lm3 gene. Carbohydrate fermentation test was used to distinguish the species of Bifidobacterium, the results revealed that the B.bifidum is more predominant than the other species; B.thermoacidophilum, B.adolescentis, B.breve, B.longum. Antibiotics susceptibility test to Staphylococcus aureus using antibiotic disc diffusion assay showed that Methicillin Resistant Staphylococcus aureus formed 70% and 30% of isolates were sensitive to the same antibiotics, also the ability of MRSA isolates to produce Biofilm and Slime layer was investigated by tube method and Congo red agar, then 10 isolates of Staphylococcus aureus were selected as efficient isolates according to the resistance of Methicillin and the high ability to produce biofilm and Slime layer. The antagonistic activity of Bifidobacterium spp was extracted and tested against these MRSA isolates, the results showed that the most isolates of Bifidobacterium spp were effective against Methicillin Resistant Staphylococcus aureus (MRSA), as well as the inhibition of growth and formation of Slime layer by using the wells and disc diffusion agar assay. The inhibition zone was between (10 - 30)mm, and the ability of bacteriocin production of Bifidobacterium spp against MRSA have been conducted by well diffusion broth method, B.bifidum3 was greater than other species in producing bacteriocin with asignificant increase (P<0.05). Furthermore the impact of bacteriocin production to inhibit the development and formation of biofilm was investigated, the results showed that the bacteriocin has ahigh activity against MRSA. The bacteriocidal and bacteriostatic effect of bacteriocin was conducted by using spectrophotometer, the results showed that these effects were bacteriostatic in the beginning of the growth MRSA while it was lethal during other time and 40 minutes is the period needed for killing MRSA bacteria.

دراسة نوعية مياه خزان الحبانية موقعيا والاستشعار عن بعد == Study of The Water Quality In Alhabbaniyah Reservoir In Situ And Remote Sensing

اسم المؤلف: احمد عطا الله حسن الفهداوي
اسم المشرف: عادل مشعان ربيع
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: البيئة المائية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:

العلاقة بين مؤشر الموت الخلـوي المبرمج (ربيطة فاس الذائبة) وقلة النطاف عند الرجال == Correlation Between Apoptotic Marker (Sfas Ligand) And Oligozoospermia In Men

اسم المؤلف: احمد حسين علي الزاملي
اسم المشرف: صباح ناصر العلوجي | انعم رشيد الصالحي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The Fibroblast associated (Fas) system in the testes has been identified as a key regulator of apoptosis, a process that greatly influences the germ cell population of the testes. and the soluble Fas Ligand (sFasL) may be a competitive inhibitor to induce apopto¬sis of the Fas baring cells. Therefore, this study measures the level of sFasL in the semen of oligozoospermic men evaluating the association between seminal plasma sFasL and spermatogenesis.A total 58 oligozoospermic men and 29 normozoospermic volunteers were included in this study. They were attending the Infertility Clinic of the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, AL - Nahrain University\ Baghdad. Their age mean was 31.77± 5.48 years and a median of 30 years. Semen samples were evaluated according to World Health Organization 2010 standard parameters. The Seminal plasma was separated from the cellular elements after centrifugation and the supernatant was transferred to plane tubes and frozen at - 20 ?C till using for measurement of sFasL. These patients were classified into patients with severe oligozoospermia (sperm concentration ?5 million / ml) which include 29 patients, and patients with mild and moderate oligozoospermia (sperm concentration ranging between 5 - 15 million / ml) which includes 29 patients as well. The control group comprised of 29 men with normospermic parameters according to WHO 2010 standard (Normal volunteers). The sFasL was measured using ELISA enzyme immunoassay for quantitative determination of sFasL Kit. Data were analyzed statistically using Analysis of variance (ANOVA), Least Significant Difference (LSD) and Correlation Coefficient (r).The sFasL level was found significantly higher (P< 0.01) in seminal plasma of oligozoospermic men, with weak correlation of the level of sFasL with the degree of severity of oligozoospermia. Based on the results of this study, it was concluded that sFasL is a novel marker found in the seminal plasma of oligozoospermic men. It's level was higher in cases of oligozoospermia regardless of its severity.

تكامل جرعة منخفضة من مبيد الترفلان مع مخلفات زهرة الشمس في مكافحة الادغال وتحسين نمو محصول الماش لتقليل الاعتماد على المبيدات والحد من مخاطرها == Integration of Reduced Dose of Trifluralin Herbicide With Sunflower Residues For Weed Control In Mungbean Field

اسم المؤلف: اﺭﻭﻯ ﻋﺒﺪ ﺍﻟﻜﺮﻳﻢ ﺗﻮﻓﻴﻖ
اسم المشرف: اﺑﺮﺍﻫﻴﻢ ﺷﻌﺒﺎﻥ ﺍﻟﺴﻌﺪﺍﻭﻱ
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: النبات - الفسلجة
الدرجة: دكتوراه
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Two field experiments, several greenhouse and laboratory experiments were conducted during the course of study to evaluate the allelopathic potential of two sunflower (Helianthus annuus L.) cultivars on companion weeds and weeds grown in mungbean (Vigna radiata L. R. Wilczek) field alone and in combination with reduced rate herbicide, and to determine the chemical and genetical bases of allelopathic traits in the test cultivars. The aim of the first experiment was to test whether the variation in weed growth between the sunflower cultivars (Shumoose and Sin Altheeb), which was observed in the field, was due to differences in their allelopathic potential. Results showed the ability of both cultivars to reduce weed population and biomass with the superiority of Shumoose cultivar in suppression of weed density at 60 and 120 days after sowing compared to Sin Altheeb. Weed biomass was significantly reduced up to 90 and 71% by Shumoose and Sin Altheeb cultivars, respectively. Stair case experiment indicated that root exudates of Shumoose cultivar showed more suppression to weeds than Sin Altheeb giving additional evidence for the superiority of Shumoose cultivar in its allelopathic weed suppression. Chemical analysis by High performance liquid chromatography indicated the presence of 9 compounds in root exudates of Sin Altheeb and 8 compounds in Shumoose, and all are Phenolic in nature. However, total of the isolated phenolics was doubled in Shumoose than in Sin Altheeb. Most of the isolated chemicals are reported to have inhibiting ability for germination and growth of plants, including weeds. Second field experiment was undertaken to explore the response of weeds and mungbean crop to soil incorporated with sunflower residues in combination with lower rate of a pre - plant herbicide (trifluralin). Sunflower residues of the two cultivars Shumoose and Sin Altheeb at 3.2 and 6.4 t ha - 1 were used either alone or in combination with trifluralin at 1.2 L ha - 1 (50% of label rate). Weedy check (control), trifluralin at full label rate (2.4 L ha - 1), and weed free treatments were also included for comparison. Plots treated with 50% of label rate of herbicide and amended with sunflower residues of cultivar Sin Altheeb recorded least weed density and dry biomass and this suppression was much greater than the residue treatments alone and more than cultivar Shumoose. Application of herbicide at 50% rate in plots amended with Sin Altheeb residue resulted yield 64 % more than with the label rate of herbicide treatment. Chromatographic analysis of sunflower amended field soil revealed the presence of several potent allelopathic compounds in the residues of both cultivars with greater quantity (355.5 µg/ml) in Sin Altheeb than in Shumoose (250.9 µg/ml). Dynamics of release, decomposition and degradation of allelochemicals into the soil showed that different compounds exhibited differential behavior for these processes. Overall quantity of allelochemicals started to increase after 2 weeks of decomposition and peaked at 4 week of decomposition (180.1 ppm) then declined sharply in their quantities thereafter. Periods indicating higher quantities of total phenolics as shown by chromatographic analysis was coincided with the periods in which higher suppressive activity against weeds grown under field conditions. Bioassay of sunflower residues decomposed in soil at different times on Echinochloa colonum L, one of the weeds dominated the mungbean field, revealed that residues of both cultivars suppressed growth of E. colonum weed. The phytotoxicity started after 2 weeks and persisted for 8 weeks. However, Sin Altheeb residues showed greater inhibition than Shumoose cultivar at the last three decomposition periods. Shumoose residues recorded appreciable reduction to the growth of test weed compared to Sin Altheeb. The effect of residues of both cultivars on chlorophyll content and ions uptake in Amaranthus retroflexus L. weed revealed that residues of both species reduced chlorophyll content of leaves and the reduction increased with the increased residue rate. Sin Altheeb cultivar residues were more inhibitory than Shumoose at the lower residue rate. In the same weed, ions uptake was significantly averted by the residues of both cultivars. P and K was significantly increased over control, while Ca, Mg an Fe were found to be decreased over control due to application of sunflower residues in soil at 8 g per kg soil. N was the only test element that did not significantly influence by the residue treatments. No significant differences were found in the test ion uptake between the test sunflower cultivars. Results of using RAPD technique on the sunflower genomic DNA revealed that all the 19 primers used in this study scored different amplification monomorphic and polymorphic bands in the tested genotypes with 12 primers generated polymorphic bands. These bands present in one cultivar and absent in another. They could be responsible for allelopathic trait or secondary compounds associated with this phenomenon. Further work is recommended to analyze the sequence of this band to find out whether it is related to allelopathic trait or not.

التنميط الجيني لفايروس التهاب الكبد نوع اي لمرضى الاصابة الحادة في محافظة المثنى - العراق == Genotyping of Hepatitis E Virus Among Patients With Acute Infection In Al - Muthanna Province / Iraq

اسم المؤلف: ازهار صبري مسلم الذهبي
اسم المشرف: رغد حربي مهدي العزاوي
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Hepatitis E Virus (HEV) infection is found worldwide. It is responsible for large outbreaks in East and South Asia. This study was carried out to identify the distribution of acute HEV infection in AlMuthanna province and then determine the genotypes responsible for the disease spreading. This is the first investigation about the occurrence of hepatitis E genotypes in Al - Muthanna province patients. The study included 270 jaundiced patients of both sexes From AlMuthanna province which included out patients in public health laboratory in Samawah city, for the period from October 2013 to June 2014. All these patients were tested for anti - HEV IgM antibodies by using enzyme linked immunosorbent assay (ELISA). In Al - Muthanna province, 72 patients (26.66 %) out of 270 patients showed positive results for anti - HEV IgM antibodies, and all those patients were tested for confirmatory test at central public health laboratories (CPHL) in Baghdad province. Those patients consist of 45 females and 27 males with age ranged between (4 - 74) years old, were all negative for routinely screened markers of Hepatitis A, Hepatitis B and Hepatitis C, Ten normal healthy individuals, used as normal control in this study. This study showed that the HEV IgM is more common among younger age group (15 - 24), with a percentage of) 41.67%(, and it was higher in females (63%) than in males (37%). Highly significant differences (p ? 0.01) appeared among age groups. The levels of liver function enzymes demonstrated significant differences (p ? 0.05) in HEV patients as compared with healthy controls. The levels of Total Serum Bilirubin (TSB) were (23.41 ± 12.38) mg/dl, Serum Aspartate transaminase (SAST) were (887.17 ± 9.87) U/l, Serum Alanine transaminase (SALT) were (859.91 ± 13.28) U/l, and the levels of Serum Alkaline phosphatase (SALP) were (206.56 ± 5.04) U/l higher than healthy controls (0.884 ± 0.05) mg/dl, (37.27 ± 4.58) U/l, (34.14 ± 7.63) U/l, (48.09 ± 15.42) U/l respectively. RNA was extracted from sera of positive anti - HEV IgM antibodies by using a QIAamp® Viral RNA Mini Kit. Nanodrop and Quantus™ Fluorometer instruments were used to determine the RNA yield and purity according to the standard kit protocol. High levels of RNA concentration were measured by Nanodrop and Quantus™ Fluorometer. Forty - six samples have high RNA concentration out of 72 samples were detected for genotypes I, III & IV by using Applied Biosystems Real time PCR 7500 machine. In this study, HEV type I and III were detected in 23 samples out of 46 samples by using Real Time PCR systems. Ten samples were positive for this test. Thirteen samples (56.52%) were negative for this test. The distribution percentage of genotypes I and III was (43.48%). The results appeared significant difference (P ? 0.05). Also by using the same technique (Real time PCR systems) the HEV type IV was detected in 23 samples out of 46 samples. Which included, 13 samples that recorded negative results by I & III genotypes kit and only 2 samples from it recorded positive results and 11 samples recorded negative result for HEV genotype IV. From other 10 samples, only 3 samples recorded positive result and 7 samples recorded negative result for HEV genotype IV. In a total, 5 samples were positive for HEV genotype IV and 18 samples (78.26%) were negative. So that the distribution percentage of genotype IV was (21.74%). In this study, the samples that showed negative results in both kits have not been tested for other genotypes. This study indicated that the acute HEV infection is highly endemic in Al - Muthanna province in Iraq. Genotypes I & III were the most distribution than genotype IV in patients from Al - Muthanna province in Iraq. These results suggest that the genotypes I & III are the main causative agents of sporadic HEV infection in Al - Muthanna province

التحري الجزيئي عن النمط الثالث لداء السكري البادئ عند النضج (MODY 3) في مرضى السكري العراقيين == Molecular Investigation of Maturity Onset Diabetes of The Young Type 3 (MODY 3) In Iraqi Diabetic Patients

اسم المؤلف: اسراء عدنان ابراهيم البغدادي
اسم المشرف: نورية عبد الحسين علي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: شملت الدراسة 63 مريضا مصابا بداء السكري غير المناعي المنشا في اعمار مبكرة من ذوي التاريخ العائلي بالاصابة بمرض السكري ممن لايعانون البدانة الذين تم اختيارهم من مرضى السكري من مراجعي المركز التخصصي لامراض الغدد الصم والسكري في الرصافة/ بغداد للفترة من ايار | This study included 63 non obese patients having early onset of non immunogenic diabetes with strong family history of diabetes selected from diabetic patients attended the specialized center of endocrinology and diabetes at Alrusafa/ Baghdad during period from the begging of May 2011 till the end of February 2013. The demographic study revealed that there is no association between the disease incidence with neither the gender nor the blood group. But there is a significant difference in the distribution of the patients according to age of the first diagnosis and gender, as the study showed that majority of male patients (53.846%) were first diagnosed with hyperglycemia below the age of 20 while the majority of females (72.972%) were first diagnosed with hyperglycemia after the age of 20.The distribution of patients according to the type of treatment also reveled association between the gender and both age of diagnosis and type of treatment of the hyperglycemia as the study showed that there is a higher percent of male patients (80.768%) using insulin at the beginning of their diagnosis with hyperglycemia or transferred later to insulin than those of females (67.565%).The present study reported for the first time in Iraq the presence of monogenic diabetes (maturity onset diabetes of the young type 3) as major cause of diabetes within non obese diabetic patients' early onset of non immunogenic diabetes with strong family history of diabetes. The sequence analysis of the hepatocyte nuclear factor 1? gene showed that Iraqis have mutational hot spot at exons 3 and 4 of this gene responsible for maturity onset diabetes of the young type 3, and that mutations in the promoter region or exons 5, 6, 8 and 9 are a rare cause of diabetes.

عزل وتشخيص المبيضات من المرضى المصابين بداء السلاق الفموي في محافظة النجف الاشرف مع دراسة جزيئية لبعض عوامل الضراوة == Isolation And Identification of Candida Spp. From Patients With Oral Thrush In Al - Najaf Province And Molecular Study of Some Virulence Factors

اسم المؤلف: ايهاب يحيى جابرالعلياوي
اسم المشرف: فاطمة عبد الحسين التميمي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: النجف
الصفحات الاولى:
المستخلص: تشمل هذه الدراسة بعض المعايير السريرية لمرضى نقص المناعة (مرض السكري والسرطان وزرع الكلى)، والمصابين بداء المبيضات الفموي والتي تضمنت العمر والجنس. الهدف من هذه الدراسة هو عزل وتشخيص انواع المبيضات. بطرق مختلفة بما في ذلك التشخيص المباشر، والتحضين في الم | The present study was conducted to isolation and identification of Candida spp. Isolated from immunocompromised patients with (Diabetes, Cancer & Kidney transplant) by different identification methods including direct examination, laboratory culture, biochemical tests and molecular method using polymerase chain reaction (PCR) technique and determine the virulence factors phenotypic to Candida spp. and genetic polymorphism in Candida albicans in secreted aspartyl proteinase (SAP2). During the period from October 2013 to February 2014, a total of 140 oral swab were collected from immunocompromised patients with attending to the three center in AL - Sadder Medical City (Oncology center and kidney center and Center for Diabetes and Endocrinology).In AL - Najaf Governorate, the samples were collected as following : - 110 mouth swabs from patients suffering from leukemia, prostate, stomach and bowel cancer, also 10 mouth swabs from kidney transplant patients and 20 mouth swabs from diabetes. The percentage of female to male was as following, female (86) 64(72.7%) infected, 22(42.3%) uninfected and male (54) 30(57.7%) uninfected, 24(27.3%) infected, with age range between (5 - 64) years. In this study, the results show that the incidence of candidiasis in women was higher than the male also the most cases of candidiasis in patients age range between (45 - 54) years. The results of the present study reveal that the prevalence of oral candidiasis from patient with cancer was more than diabetes & kidney transplant patients which was 72(81.18%), 12(13.65%) & 4(4.54%) respectively. When culturing oral samples on the primary isolation media Sabouroud dextrose agar (SDA) and the secondary media on CHROM agar, The results reveal the presence of 88 isolates belong to Candida spp.which include : - 42 (47.68%) belong to Candida albicans, 20 (22.72%) belong to Candida. dubliniensis, 18 (20.5%)belong to Candida krusei, 6 (6.8%) belong to Candida glabrata & 2 (2.3%) belong to Candida parapsilosis. According to this result, Candida albicans was found the most frequently isolated species and the occurrence of the other non - albicans isolates such as Candida dubliniensis and which followed Candida albicans in frequency of the isolates in oral candidiasis. In this study, the ability of C.albicans & non - albicans was tested to produce some virulence factors such as production of enzymes phospholipase, lipase, germ tube, chlamydospore & biofilms when culturing Candida spp. on different media. The results of the culture show that Candida albicans produces all virulence factor included germ tube, chlamydospore, biofilms & phospholipase. Some species share several phenotypic characteristics ability to produce enzymes of phospholipase such as C.krusei while C.dubliniensis produce chlamydospore, when culturing in the Casein agar & produce biofilms, In addition the molecular tests to was used for Candida albicans confirm the ability to secreted aspartyl proteinase (ASP2). This study showed the amplified from the Candida spp. as following : - C.albicans 306 bp, C.glabrata 594 bp, C.parapsliosis 387 bp, C.krusei 651 bp & C.dubliniensis 451 bp.

الكشف عن بعض عوامل البيئة الداخلية للخلايا السرطانية للنساء العراقيات المصابات بسرطان الثدي == Detection of Some Microenvironment Factors In Tissue Samples of Iraqi Women With Breast Cancer

اسم المؤلف: فاطمة سمير عبد الرزاق الزبيدي
اسم المشرف: اخلاص مشرف عيدان
الموضوع العام: علوم الحياة
السنة: 2014
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Breast cancer (BC) is the most common cause of cancer - related deaths in women. It continues to rank as one of the top killer of women. This cancer increased in frequency in the last years in Iraq. The aim of this study was to shed light on the immunohistochemical for some factors that could be affect on development of microenvironment in breast cancer of Iraqi patients. And these factors include CD133 as a marker for breast cancer stem cells (BCSs), and also studying tumor necrosis factor alpha (TNF - ?) and transforming growth factor beta (TGF - ?). 53 samples Formalin - fixed, paraffin - embedded (FFPE) wax block appeared their ages were range from 29 - 70 year with a mean age of 48.45 years. 32 patients with breast carcinoma and 21 patients with benign breast lesions were included in this study for comparison. The results of this study show that the CD133 positive expression was found in (56.2%) of Iraqi breast cancer cases. Also the result of this study show that (62.5%) positive expression from both (TNF - ? &TGF - ?) of breast cancer cases, compared with sample of benign breast lesion. The results show (52.3%) positive expression of TNF - ? and (28.57%) positive expression of TGF - ? of samples with benign breast lesion, there is a significant different between studied samples, compared with (19.05%) samples positive expression of CD133 of sample with benign breast lesion so there is a significant different between the samples with breast cancer and benign breast lesion. Also the results show there is a positive relationship correlation between (TGF - ?) expression and (TNF - ?) expression, while there were no relationship correlation between (TGF - ?) and CD133 and no relationship correlation between TNF - ? and CD133. The results show there is a positive correlation between the grade and breast cancer with the three different expressions of marker but in different strongest correlations between (TNF - ?) and (TGF - ?) with the graded but this correlation becomes weak with CD133 marker the value of significant. According to the relationship in breast cancer case in this study between the studied markers and stage of case it shows (TNF - ?) has a strong positive correlation while the correlation appear week between the stage of this studies case and end each of TGF - ? and CD133. For this we concluded from the results there high expression of CD133 and TNF - ? indicators and TGF - ?. CD133 could use in diagnosis of the cancer cell and the high expression of TNF - ? & TGF - ? indicate that these factor play important roles in tumor microenvironment metastasis. And the strong correlation between the expression of these markers with grade and stage of breast cancer

دراسة بعض الجوانب المناعية والبكتريولوجية لمرضى ذات الرئة Pneumonia == Study Some Immunological And Bacteriological Aspects of Pneumonia Patients

اسم المؤلف: كرم رياض حسن الجراح
اسم المشرف: رسمية عبد ابو ريشة
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: جمعت 120 عينة قشع من اشخاص مصابين بمرض ذات الرئه للمدة من ايار 2013 الى شباط 2014 من ثلاثة مستشفيات في مدينة بغداد وشملت مستشفى اليرموك التعليمي, مستشفى دار التمريض الخاص, مستشفى بغداد التعليمي. شخصت العينات باستخدام الفحوص البايوكيميائية, نظامApi 20 E و| From May 2013 to Feburey 2014, 120 sputum and Aspiration samples of patients with pneumonia disease were collected from different hospitals in Baghdad included : Al Yarmouk Teaching Hospital, Hospital Nursing Home sector and Baghdad Teaching Hospital. All samples were diagnosed by biochemical tests, Api 20 E and Api20 strep. Systems, The results was found to be 28 isolates (23.3%) belong to K.pneumoniae, 26 isolates (21.7 %) belong to S.pneumonia while 66 isolates(55%) belong to causes : E.coli, Pseudomonas sp., Moraxella catrrhalis, S.pyogens, Monilia and S.aureus. From (55) blood samples of pneumonia patients and 30 blood samples from healthy individuals, number of white blood cell (Neutrophil) counts were measured, percentage of Neutrophil cell count in female (53%) higher than the cell count in male(47%). Some markers of pneumonia disease were studied such as ratio of infection between male and female, chronic and acute cases and smoker and non smoker, the results were showed the ratio of infection in female more than in male, (60%, 40%), respectively. And number of acute cases of pneumonia patients 41(74.55%) more than chronic cases 14(25.45%). While infection ratio in smoker patients more than nonsmoker patients at ratio 24(57.14%)18(42.86%), respectively. The result of interleukin - 8 level through acute and chronic phases in pneumonia patients sera was showed high level in patients compared with healthy persons (86.4403 ± 25.50919 vs. 58.7636 ± 4.73152 pg / ml, respectively) with a non significant difference statistically (P?0.05). Also in the age group (age 15 - 60years), The results showed the results interleukin 8 levels higher than the age groups of (2month - 3years) and (age 60 - 85) (93.63 ± 51.65, 68.88 ± 17.17, 65.68 ± 11.73 pg / ml, respectively) with non significant difference (P?0.05). The result of Leukotriene B4 level through acute and chronic phases in pneumonia patients sera showed high level in patients compared with healthy persons (36.00 ± 3, 82 vs. 25.96 ± 4.44 pg / ml, respectively) with a significant difference (P?0.05). Also in the age group (two months - 3 years) were showed the results interleukin 8 levels higher than the age groups (age 15 - 60) (age (60 - 85)(33.61 ± 11.06, 19.29 ± 4.67, 16.86 ± 4.51 pg / ml respectively) with no siginificant difference (P?0.05), the correlation coeifficient between interleukin - 8 and leukotriene B4 was (0.12) with no siginificant difference. The phagocytic activity was determined in pneumonia patients sera according to measurement C3 protein level through acute and chronic phases, the result were showed the ratio of C3 protein levels in healthy persons more than in patients (135.93 ± 12.74, 133.19 ± 12.01 pg / ml, respectively) with a difference was not significant statistically (P?0.05).

التنميط الوراثي لمستضدات التطابق النسيجي في عينة عراقية من مرضى السكري النوع الثاني == Hla Genotyping In A Sample of Iraqi Type 2 Diabetes Mellitus Patients

اسم المؤلف: احمد كاظم محمد
اسم المشرف: محمد ابراهيم نادر | بتول حسن الغرابي
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: دكتوراه
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: داء السكري مرض واسع الانتشارعالميا تختلف نسبة الاصابة بين البلدان المتطورة والنامية ويعتبر المسبب الرئيسي للاعاقة والموت في العالم.داء السكري النوع الثاني هو الاكثر حدوثا حيث تبلغ نسبة الاصابة (90 - 95%) من مجموع المصابين بالسكري للانواع الثلاثة الرئيسية. | Sixty of non insulin dependent diabetes mellitus (NIDDM) patients who were diagnosed according to American Diabetes Association criteria (ADA) 2007 were selected from the specialized center of endocrinology and diabetes (Baghdad AL - Russafa Health Directorate) during the period between first of May 2013 to last of October 2013.Their age ranged 35 - 70 years. Fourty apparently healthy individuals their age ranged (35 - 70) years were considered as control. Enzymatic colorimetric methods used for measured FBS (fasting blood sugar) and HbA1c (glycohemoglobin) and enzyme linked immunosorbent assay (ELISA) for hormones and enzyme markers. Fasting blood sugar revealed high significant in patients with median (11.6 mmol/L vs. 5.9 mmol/L) and (P<0.001) in comparison to control groups.Elevation of glycated hemoglobin (HbA1c) with mean (9% vs. 5.5%) in comparison to control groups. Another reliable marker are the concentration Adpionectine hormone, Insulin hormone and adenosine deaminase activity the results of those estimated significantly difference between levels mean (20.7 vs. 34 ng /ml) in patients compared to healthy subjects (P<0.001) for adiponectin ; mean (106.6 vs. 59.3 ng/ml) for ADA (adenosine deaminase) with (P<0.001) and the median (12.1 vs. 16 uIU/ml) for insulin hormone with (P 0.001). In order to investigate the accuracy of previously mentioned tests, a statistical analysis [Receiver - Operator Characteristics (ROC)] has been applied to show the accuracy, specificity and sensitivity of the tests under test.This analysis revealed that serum ADA activity is the best marker with highly specificity 100%, sensitivity 100%, and accuracy 100% while; FBS was the best test with highly specificity 100%, sensitivity 100% and 100% accuracy in comparison with other tests. It was denoted that type2 DM was associated with certain HLA class II alleles were analyzed for their genotyping by Polymerase Chain Reaction - Sequences Specific Olegionucleotide (PCR - SSO) technique. The present study revealed that diabetic patients were positively associated with HLA - DQB1*0201 (83% vs. 5.0%) which is the most prevalent in patients followed by DRB1*1137 (46.7% vs. 0.0%); DRB1*0401 (41.7% vs. 2.5%), and DRB1*1306(15% vs.0.0%) while HLA, A*0201;B*3559;Cw*0410 and DQB1*0501 is negatively associated in type 2DM in comparison with healthy control groups.This study has shown that there is no significant association between FBS, HbA1c, serum insulin, HOMA2(Homeostatic Model Assessment2) ? - function, HOMA2 - IR, serum adiponectin, serum ADA and HLA alleles(DQB1*0201, DRB1*1137, DRB1*0401, DQB1*0501, DRB1*1306) in spite the significant associated between FBS and serum ADA and HLA - DRB1*0701 allele with (P 0.021, P 0.008) respectively.The current result concluded that there may be an important role for HLA genotyping in arising the chance for enhancing the susceptibility for either disease development or protection against its initiation.

انشاء وتوصيف لخط سرطان الثدي الخلوي العراقي == Establishment And Characterization of Iraqi Breast Cancer Cell Line

اسم المؤلف: مرتضى عادل الشامي
اسم المشرف: محفوظة عباس عمران | احمد مجيد الشمري
الموضوع العام: علوم الحياة
السنة: 2014
الموضوع الدقيق: التقنيات الاحيائية
الدرجة: ماجستير
اللغة: الانكليزية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: Several primary cultures were initiated from different samples obtained from Iraqi female patients of breast tumor, one sample was successful, and this sample was histological diagnosed as breast cancer infiltrating ductal carcinoma.The cell suspension was cultured in tissue culture flask and confluent monolayer achieved after 16 days from primary culture. The continuous subculture was done in grown cells in tissue culture flask each 48 - 96 hrs. Between subculture to other until across 50 passages through11months.In our current study different experiments were done to characterize the cultured continuous cells, which are studying the growth curve of the new established cell line and calculating the population doubling time that have been 22 hours.Furthermore, a morphological study was carried out by staining the cells with hematoxilin and eosin dyes. The cells were elongated multi - polar epithelial like cells with nuclear polymorphism and multi - nuclei, in addition to high nuclear to cytoplasm ratio, all these characters of the malignant tumor cells.The Cytogenetic study showed chromosomal aberrations with many numerical changes among the tumor cells and abnormal structure gives chromosomes with unknown origin called marker chromosome. In furthermore the G - band stained of normal 46XX chromosome was done to facilities the comparisons between chromosomes of the new established breast cancer cell line and normal chromosomes aberrationsImmunocytochemistry examination was done for the tumor cells grown in multi well tissue culture slide chamber to detect the presence of some hormonal receptors (ER and PR) gives negative result, and to test Her - neu2 gene that gives week positive result.
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