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دراسة تاثيرات سم الزيرالينون في حبوب الذرة الصفراء والحنطة على بعض المعايير الحيوية لدى اناث الجرذ الابيض وامكانية المعالجة الحيوية للحد من تلك التاثيرات == Study The Effects of Zearalenone Toxin In Maize And Wheat Grain On Some Vital Parameter Among Female White Rat And The Possibility of Biological Treatment To Reduce These Effects

اسم المؤلف: محمد فخري حسين الخفاجي
اسم المشرف: سامي عبد الرضا علي الجميلي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات - السموم
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: هدفت هذه الدراسة الى التحري عن سم الزيرالينون في حبوب الذرة الصفراء (Zea mays) والحنطة(Triticum aestivum) في محافظتي كربلاء وبابل واختبار فاعلية المستحضر الحيوي لبكتريا subtilis Bacillus في حماية حبوب الذرة الصفراء والحنطة من الاصابة بالفطر Fusarium grami | This study aimed to investigate Zearalenone toxin in yellow corn (Zea mays), wheat (Triticum aestivum) in the provinces of Kerbala, Babil and test the effectiveness of the vital preparation for bacteria Bacillus subtilis in the protection of maize and wheat grain from getting the fungus Fusarium graminearum under normal storage conditions. Moreover been conducting a study on the possibility of employing the vaccine bacteria B. subtilis after her killing in thermally protect vital systems for female white rat poison from the effects Zearalenone toxic.Isolation and diagnosis results showed the presence of many different races and fungal species and was the most presence in maize and wheat grain genus Aspergillus spp. and Fusarium spp. Emergence rates reached 58% and 33.3% respectively in maize grain and by 66.6% and 25% respectively in the grain of wheat. The test demonstrated the ability isolates fungus Fusarium spp. results To produce Zearalenone toxin using technology (TLC) Thin Layer Chromatography having 10 isolates that produce the poison out of 12 isolation or by 83.3%. On the other hand diagnosed fungal species F. graminearum and F. moniliform and F. culmorum as productive poison Zearalenone while isolation F.napiform were not able to produce the toxin. The results of the investigation of contamination of samples of yellow corn and wheat Zearalenone using technology TLC presence of contamination and the two rates stood at 41.6% in maize and 25% in wheat grain cereals The results of the study of the effect of pH and sodium chloride in the growth of fungus F. graminearum that this fungus can grow a wide range of pH and ranges from 12 - 6 as well as afford salt levels ranging between 1 - 4 PPT Per 100 ml. This study demonstrated the effectiveness of the vital preparation for the bacteria B. subtilis in the protection of maize and wheat grain from injury F. graminearum under normal storage conditions for a period of three months, not the effectiveness of these bacteria to protect maize and wheat grain in the normal storage conditions are limited but had a distinct role in the protection of critical systems to female white rat when treated those animals vaccinated with bacteria murdered thermally followed congection Zearalenone toxin as any symptoms in some biochemical and physiological blood parameter studied as well as the safety of the investigated organs such as the liver, kidney, Small intestine, ovary and uterus of any medical condition did not appear at a time appeared in which variations in satisfying those criteria have studied female albino rat treatment Zearalenone toxin only.

دراسة بيئة وتنوع الهائمات الحيوانية في نهر الغراف واسط - العراق == Ecology And Diversity Study of Zooplankton In Al - Gharraf River Wasit - Iraq

اسم المؤلف: رشا موحان سلمان
اسم المشرف: جميل سعد السراي | مهند رمزي نشات
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: واسط
الصفحات الاولى:
المستخلص: جريت دراسة بيئية للهائمات الحيوانية في نهر الغراف, وموقعه في الجزء الجنوبي الشرقي من العراق, تحيط به مساحات شاسعة وخصبة من الاراضي الزراعية. هدفت الدراسة الى التنوع الاحيائي للهائمات الحيوانية, من خلال قيم بعض ادلة التنوع الاحيائي, وكذلك دراسة تاثيربعض ال | An ecological study of zooplankton was carried out on the Al - Gharraf River, that located in the south eastern sector of Iraq and surrounded by vast and agricultural land to the aim of this study are biodiversity of zooplankton by through some of biodiversity index values of some physical and chemical characteristics of water on the biodiversity and abundance of Al - Gharraf zooplankton. The samples of the study have been collected monthly for the period from August 2014 untill July 2015 at three selected stations in the river; Station one is located at 500 m of the AL - KUT Dam. The second is situated at a distance of 15 Km away from the station one at (Kut - Muwafaqiya) and the latter station is located at 18 Km apart from the second one upon entering the river to Muwafaqiya The study was achieved by measurement of some physical and chemical properties of water were analyzed the parameters studied included Air temperature (11 to 38.6) ?C, water temperature (12.1 to 29.1) ?C, pH (7.2 to 7.9), EC (490 to 1860) µS/cm, Salinity (0.31 to 1.19) ‰, DO (5 to 9.1) mg/L, BOD5 (0.2 to 7.1) mg/L, Turbidity (4.69 to 74) NTU, TDS (0.26 to 0.99) g/L, TSS (0.005 to 5.9) mg/L, TH (388 to 520) mg/L, Ca+2 (100.2 to 180.36) mg/L, Mg+2 (3.69 to 52.07) mg/L and NO3 - 2 (0.034 to 3.58) mg/L and PO4 - 2 (0.011 to 1. 25) mg/L., Bicarbonate (130 to 185) mg/L, Sulfate (80 to 180) mg/L, the percentage of oxygen saturation (48.59 to 93.82) %, Water flow (14.28 to 76.92)cm\sec. and total Alkalinity(119 to 274)mg/L.The study also included biological aspects using density, Rrelative abundance index (Ra), Constancy index (S), Species richness index (D), Shanon - Weiner index (H), Species uniformity index (E) and Jaccard presence - community were also employed to determine the values of species composition, density and zooplankton biodiversity in the study area. In the current study about 113 Taxonomic units of zooplankton were identified, the rotifer was dominate group including 64 taxa to rotifera, 34 taxa belonging to cladocera and 15 taxa to copepod.The Zooplankton showed high density in river 8332.62 Ind. /m3 during April 2015 while lower465.32Ind. /m3 in Sebtember 2014 also the rotifer recorder density from198.66 to 7865.98 Ind/m3, cladocera density from 0 to 233.32 Ind/m3and copepod density from 66.33 to 2932.65 Ind/m3.The results of the relative abundance index showed that the species rotifera : neplunia Rotaria. Keratella valga Brachionus angularis, Keratella cochlearis dolicopter While as from Cladocera, Alona rectangular, Polyarthra Alonella excise, , Moina affinis, , Bosmina longirostris Ceriodaphnia rigaudi Chydorus piger, Scapholebrus kigni. Simocephalus sevrulatu and the copepods : Immature Harpacticoida, Paracyclo fimbriatus, Nitocra sp., Nitocra spinipe, , Harpaticoida sp. and Nauplii were more abundant in Al - Gharraf river. also the results of Constancy index showed Six taxa belonged to zooplankton, which were considered "Constant" at stations However the other taxonomy units varied from "accessory" and "accidental" taxonomy units in study stations. The values of the Species richness index of rotifer varied from 0 to 5.42 and cladocera varied from 0 to1.41 and copepod from 0 to 2.70 The highest percentage of similarity 48.148% for Zooplankton were reported between stations 1 and 2. The lowest similarity reaching 35.412% recorded between stations 2 and 3. The Shanon - Weiner index of total Zooplankton varied from0.4to 2.5 bit/ind while the Shanon index of Rotifera from1.05 to 2.61 bit/Ind., Cladocera varied from 0.4to 1.38 bit/Ind. and Copeoda varied from 0.11 to 1.76 bit/Ind., The uniformity index of total Zooplankton varied from0.17 to 1.01and Rotifer varied from 0.4 to 0.9, Cladocera from 0.4 to 1 and Copeoda from 0.08 to 0.99 These high values indicate that there is no ecological stress on zooplankton in the study area

دراسة بايوكيميائية لمكونات الاكياس العدرية المستاصلة من الاغنام والابقار في محافظة ديالى == Biochemical Study of Hydatid Cysts Component Isolated From Sheep And Cows In Diyala Province

اسم المؤلف: غزوان مهدي جعفر الربيعي
اسم المشرف: نغم ياسين كاظم البياتي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: اجريت الدراسة الحالية في محافظة ديالى للفترة من بداية شهر ايلول لغاية نهاية شهر تشرين الثاني لعام 2014. تم جمع 40 كيسا عدريا من الاغنام والابقار (34 عينة من الاغنام و6 عينات من الابقار). تم اجراء التحليل الكمي لبعض العناصر والمركبات الكيميائية فضلا عن الا | The current study was carried out in Diyala province during the period from September to December 2014. Fourty hydatid cysts were collected from sheep and cows (thirty four cyst from sheep and six cysts from cows). Quantitative analysis was done for some elements and chemical composition of hydatid cyst fluids, laminated layer of cysts and the tissues surrounding cysts isolated from lungs and livers of cows and sheep, in addition to the adipose tissues of sheep. The results revealed that there was no significant difference (p>0.05) in Na, Ca, K, Mg and Fe elements in the fluids of hydatid cysts isolated from livers and lungs and adipose tissues of sheep and cows, while in sheep, Na showed (p>0.05) the highest concentration (4.83±0.19 mg/dl) in lung tissues comparison with the adipose tissues and livers (4.73±0.10 and 4.57±0.17 mg/dl respectively). The highest concentration of Fe (p?0.001) was in liver tissue (3.80±1.28 mg/dl). In addition, the highest concentration of K (p?0.001) was in walls layers of hydatid cysts isolated from liver (3.61±0.36 mg/dl) comparison with lung and adipose tissue (3.60±0.39 and 2.64±0.21 mg/dl, respectively). In cows, the highest concentration of Na (p?0.05) was recorded in lung tissue (3.85±0.84 mg/dl) comparison with liver, while the highest concentration of K (p?0.05) was in liver tissue (5.40±0.27 mg/dl) in comparison with lung tissue (4.76±0.20 mg/dl). The highest concentrations of creatinine, cholesterol and triglycerides were recorded (p?0.001) in hydatid fluids of cysts isolated from liver (47.24±2.25, 8.29±0.83 and 0.34±0.01 mmol/l, respectively) in comparison with the fluids of hydatid cysts isolated from lungs (17.80±1.79, 0.43±0.36, 0.12±0.00 mmol/l, respectively). In addition, the highest concentrations of urea, total protein and glucose were recorded (p?0.001) in fluids of hydatid cysts isolated from sheep lungs (2.15±0.03, 0.68±0.02, 31.41±0.72 mmol/l, respectively). In cows, the highest concentrations of creatinine, cholesterol and triglycerides were found in fluids of hydatid cysts isolated from lungs (13.24±2.02, 0.12±0.00, 0.12±0.00 mmol/l, respectively) in comparison with those isolated from liver. In addition, the concentration of glucose in the fluids of hydatid cyst isolated from cow lungs was higher than in those isolated from the liver (0.62±0.00, 0.45±0.00 mmol/l, respectively). The current results revealed that the fluids of hydatid cysts isolated from liver showed (p?0.001) the highest concentrations of amino acids {proline (98, 82±3.07), tyrosine (171.15±4.9) and lysine (25.39±9.29) mg/dl), and (p?0.05) for methionine (91.92±1.11), cysteine (68.97±1.61), phenylalanine (21.53±10.80) in comparison with fluids of hydatid cysts isolated from lungs and adipose tissues. For adipose tissues, (p?0.001) the highest concentrations of glutamate (58.48±6.61 mg/dl), glutamine (53.53±4.96 mg/dl), serine (57.30±1.04 mg/dl), histidine (41.64±8.71 mg/dl), threonine (52.81±5.14 mg/dl), alanine (109.81±5.39 mg/dl) and leucine (34.81±1.30 mg/dl) in comparison with fluids of hydatid cysts isolated from livers and lungs. The arginine and valine (p?0.05) were the highest in the fluids of hydatid cyst of lungs (24.39±4.27, 100.72±10.82 mg/dl, respectively) in comparison with those isolated from the livers and adipose tissues. As for the concentration of amino acids in the surrounding tissue of hydatid cysts, the hepatic tissue recorded (p?0.001) the highest concentration of acids aspartic (105.52±5.14, glutamic (35.43±9.46 mg/dl) glutamine (114.72±5.02 mg/dl) Glycine (219.58±8.59 mg/dl) serine (32.97±1.10 mg/dl) arginine (47.90±1.04 mg/dl) threonine (56.03±3.94 mg/dl) alnin (17.74±4.74 mg/dl) proline (141.89±5.84 mg/dl) valine (202.81±8.45 mg/dl) methionine (225.99±5.72 mg/dl) sistine (109.39±3.61 mg/dl) Isoleucine (28.13±3.23 mg/dl) phenylalanine (77.92±2.93 mg/dl) respectively, compared with the tissue of the lung and adipose tissue. While recorded (p?0.001) the lung tissue highest concentration of acid histidine (40.94±2.65 mg/dl) and lysine (52.88± 2.16 mg/dl) mg/dl, respectively, compared with the liver tissue and adipose tissue. As for leucine (114.46±3.27 mg/dl) recorded (p?0.001) in adipose tissue highest concentration him compared with liver and lung tissues. As for the concentration of amino acids in the cyst wall of the of hydatid cysts, the cyst wall recorded (p?0.001) isolated from hepatic tissue highest concentration of acids (aspartic (261.38±8.80 mg/dl) glutamine (270.75±1.75 mg/dl) histidine (106.04±5.43 mg/dl) glycine (129.18±5.08 mg/dl) arginine (39.77±2.51) threonine (30.43± 4.63 mg/dl) alnin (30.54±3.25 mg / dl) methionine (182.50 ±1.50 mg /dl) proline (36.29±9.54 mg/dl) sistine (199.49±6.33 mg/dl) valine (154.63±6.98 mg/dl) Isoleucine (66.10±7.71 mg/dl) leucine (104.22 ± 3.94 mg/dl) phenylalanine (137.62±1.32 mg / dl) respectively, compared with cyst wall isolated from lung tissue and adipose tissue. As for the Lysine (33.62±2.5 mg/dl) recorded (p?0.001) highest concentration in the cyst wall isolated from lung tissue compared with cyst wall isolated from the liver and adipose tissue. As for the tyrosine (254.21±3.84) and glutamic (57.49±1.75) recorded (p?0.001) the highest concentration of them in the cyst wall isolated from adipose tissue compared with cyst wall isolated from lung tissue and the liver. For the concentration of amino acids in the fluids of hydatid cysts isolated from cow liver and lungs, the results showed (p?0.05) that the highest concentrations of alanine (85.88±1.42), tyrosine (21.41±0.39) and methionine (170.47±1.19) were recorded in fluids of lung cysts in comparison with the fluids of liver hydatid cysts. In contrast, there were no significant differences (p?0.05) in the concentrations of (aspartic, glutamic, glutamine, glycine, serine, histidine, arginine, threonine, proline, valine, sistine, leucine, phenylalanine, alnin and lysine between the hydatid fluids isolated from cow liver and lungs. As for the concentration of amino acids in the surrounding tissue of hydatid cysts cows show (p?0.05) that amino acids aspartic (288.47±10.13 mg/dl) and glutamine (270.79±2.97 mg/dl) recorded highest concentration in liver tissue compared with lung tissue. While recorded amino acid (p?0.05) alanine (230.58±1.38 mg/dl) proline (124.87±3.53 mg/dl) tyrosine (257.29±1.76 mg/dl) Isoleucine (296.44±2.06 mg/dl) and leucine (222.44±4.46 mg/dl) the highest concentration in the lung tissue compared with liver tissue. While no significant difference did not recorded in the other amino acids. As for the concentration of amino acids in the cyst wall showed cyst wall isolated from the lung tissue (p?0.05) of the highest concentration of acids valine (135.89±1.01 mg/dl) and leucine (169.65±2.64 mg/dl) compared with cyst wall isolated from hepatic cyst. While no significant difference did not recorded in other amino acids.

دراسة نسجية وكيمياء نسجية للقناة الهضمية في طائر فاختة النخيل Streptopelia senegalensis == Histological And Histochemical Study of The Alimentary Canal In Laughing Dove Streptopelia Senegalensis

اسم المؤلف: خولة خلف حسون صادق
اسم المشرف: مختار خميس محمد سعيد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تمثل الدراسة الحالية دراسة التركيب المظهري والنسجي وكيميائية النسيج للقناة الهضمية للحمام البري فاختة النخيل Streptopelia senegalensis, لثمان عينات من النماذج الحية البالغة من الحمام البري التي تم اصطيادها حية باطوال مختلفة, وبعد تخديرها بمادة الكلورفورم, | The present study represent the Anatomical, Histological and Histochemical structures of the alimentary canal in the laughing dove Streptopelia senegalensis, for eight adult specimens of these wild birds were trapped alive in different lengths, The specimens were anaesthetized by chloroform, and then carefully dissected for studying the gross anatomy, the alimentary canal from the proximal esophagus to the cloaca was carefully removed from the body cavity, lengths have been taken for alimentary canal and for each part of it, the alimentary canal divided into small pieces for each part, then fixated with Bouin's solution for general histological studies.Transverse sections of the different studied samples were cut at thickness of 5 - 7microns and the prepared sections were stained with routine stains Haematoxylin and Eosina and special stains for histochemical studies ( (Periodic acid - Schiff (PAS) technique, Alcian blue - PAS method and the Mercury bromophenol blue) ). The present Anatomical study showed the esophagus as long tubular organ, the cervical part of it is longer than the thoracic part and the crop was merely enlargement sac in shape which locate at the entrance of the thorax. The stomach is differentiated into a glandular proventriculus and a muscular ventriculus or gizzard.The small intestine distinguish into duodenum, jejunum and ileum which is the longest part of it, whereas the caeca are very small pair, and the rectum is short tube which end with cloaca, the last part of the alimentary tract. The histological study revealed that the alimentary tract composed of usual four layers; tunica mucosa, tunica sub mucosa, tunica muscular and tunica adventitia or serosa. The mucosal layer of esophagus was arranged in folds which was longer in the cervical part and constituted by nonkertenized stratified squamous epithelium, esophageal glands found just in submucosal tunica of the thoracic part. The crop has the same histological structure as in esophagus but it’s folds are less high than them in esophagus and there is no glands on it. The superior gastric glands of proventriculus were simple tubular glands and the deep gastric glands were compound alveoli glands which involve the submucosa tunica.A thick layer of koilen covers the mucosa of the ventriculus it’s lamina propria had loose connective tissue with simple alveolar glands. The villi of small intestine become shorter toward the rectum, which lining by simple columnar epithelium with striated border, and contain goblet cells which gradually increase in number frequently from the duodenum to the rectum. The histochemical studies revealed that PAS and alcian blue / PAS stains were given positive react in the esophageal glands, mucosa layer of esophagus, crop, stomach, and superior gastric glands and the goblet cells of small intestine and rectum. whereas the alcian blue / PAS were givin weak react in deep gastric glands in the provetriculus and gastric gland of gizzard but goblet cells were givin positive react in small intestine and rectum.Proteins were observed in the mucosa layer of esophagus, glandular stomach, intestine and rectum, when they had taken positive react with Mercuric bromophenol blue stain, but the reaction appeared weak in esophagea glands, intestinal glands and goblet cells.

البروتين التفاعلي - C عالي الحساسية, الانترلوكين6 والفيبرينوجين كدليل على امراض القلب التاجية == Highly - Sensitivity C - Reactive Protein, Interleukin6 And Fibrinogen As Predictor of Coronary Heart Disease

اسم المؤلف: فرح حقي عزت
اسم المشرف: حميد محمود مجيد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: يعد مرض القلب التاجي (CHD) Coronary heart disease من الامراض متعددة الاسباب والتي تثار حولها العديد من التساؤلات، الا ان ارتفاع مستويات بروتينات الطور الحاد مثل البروتين التفاعلي C عالي الحساسية hs - CRP والفيبرينوجين والحركيات الخلوية مثل انترلوكين6 - (I | Coronary heart disease (CHD) considered a multifactorial disease. raising many questions about the early marker of the disease. And these include high sensitivity C - reactive protein (hs - CRP) fibrinogen and cytokines such as interleukin - 6 (IL - 6) considered a predictors of coronary heart event. and to reveal their roles in cardiac events we constructed this study. we prepared a special from for each patient and it include : age, sex, hieght, weight, and measurement of systolic blood pressure, diastolic blood pressure, fasting blood sugar, total cholesterol, triglycerides, high density lipoprotein, low density lipoprotein, very low density lipoprotein, high sensitive C - reactive protein, fibrinogen, interleukin - 6. The study included two groups : The first group included 120 people suffering from coronary heart disease and admitted to the coronary care unit (CCU) in Baquba Teaching Hospital aged between 31 - 70 years for the period from the first of November 2014 and first of May until 2015. The second group of 66 healthy people aged between 31 - 70 years for the same period. The study results showed : The existence of high statistical difference with probability of P<0.01 in the body mass index, systolic blood pressure, fasting blood sugar, total cholesterol, triglycerides, low density lipoprotein, very low density lipoprotein, high sensitive C - reactive protein, fibrinogen, interleukin - 6. while showed significant decrease <0.01 in the level of High density lipoprotein and high level of P<0.05 in the diastolic blood pressure in coronary heart disease patients compared to the control group. wich predict an increased mortality in patients

بعض المؤشرات المناعية عند مرضى الحروق في مدينة بعقوبة == Some Immunological Parameters In Burn Patients In Baquba City

اسم المؤلف: عبد الله خزعل محسن القيسي
اسم المشرف: عباس عبود فرحان الدليمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: اجريت الدراسة في مختبرات مستشفى بعقوبة التعليمي التابعة لمحافظة ديالى خلال المدة الزمنية المحصورة من 1 /2014/10 الى 2015/ 7 /1 وتضمنت هذه الدراسة ثلاث مجاميع هي مجموعة المرضى تالفت من 70 شخصا من المرضى الراقدين في ردهة الحروق التابعة للمستشفى اعلاه بواقع | The study was conducted at Baquba teaching hospital in Diyala province during the period October 2014 - July 2015. The study included three groups, a total of 70 patients who were admitted at burn department they were 33 (47.14%) female and 37 (52.85%) male with age range from (1 - 60) year, 40 (57.14%) of 1 - 20 year, 25 (35.71%) of 21 - 40 year and 5 (7.14%) of 41 - 60 year, and three degrees of burns, 15 (21.42%) of the first degree of burn, 34 (48.57%) of the second degree of burn and 21 (30%) of the third degree of burn. The polices of the two people who did not suffer from any medical condition, the first group consisted of 30 people to 14 (46.66%) female and 16 (53.33%) male. And the second set especially cellular Dynamics consist of 10 people included 5 (50%) females and 5 (50%) male, note that sets the polices were distributed to the same age groups above. C - Reactive protein is positive measured effectively using latex examination and rely on the presence of altlasn from non existence, while conducted examinations alghlobulinat immuneglobulins IgG and IgM, and complement components C3 and C4 by single Radial Immuno diffusion and conducted tests kinematics cellular IL - 2 and IL - 6 using adsorption - linked immunosorbent assay technique. The results of the study showed significant difference when level (P < 0.05) for CRP value reaching up to his cause the immune index in patients group compared with the control group. While results of IgG concentration showed great differences at the level of (P < 0.01) in female patients with average (1103.02 mg/dl) and males average (1136.05 mg/dl) compared to the control group females average (977.90 mg/dl) and males average (1038.00 mg/dl). While results of IgM concentration showed great differences (P<0.01) in female patients with average range (242.84 mg/dl) and males average (233.50 mg/dl) compared to the control group females average (124.37 mg/dl) and males an average of (93.10 mg/dl). While results of C3 concertration showed significant differences (P<0.05) in female patients with average range (94.87 mg/dl) and males an average of (131.40 mg/dl), compared to the control group females average (98.6 mg/dl) and males average (93.10 mg/dl). While the results of C4 concentration showed significant differences (P<0.05) in female patients with average range (41.31 mg/dl) and male average (37.78 mg/dl) compared to the control group females average (24.62 mg/dl) and males an average of (25.50 mg/dl). The results of IL - 2 concentration showed great differences (P<0.05) in male patients only average (30.16 pg/ml) compared to the control group average of (29.66 pg/ml). While results of IL - 6 concentration showed significant differences (P<0.01) in female patients with average range (63.39 pg/ml) average male (66.47 pg/ml) compared to the control group females average (2.48 pg/ml) and males average (22.80 pg/ml). Moreover the results of immunological indices the significant differences between age groups and burning scores of people with burns in comparison with the control group

دراسة عدد من المعايير الكيموحيوية والاجهاد التاكسدي لدى المرضى المصابين ببعض امراض القلب == The Study of Some of Biochemical Criteria And Oxidative Stress in Patients With Certain Heart Diseases

اسم المؤلف: منى احمد لفتة النيساني
اسم المشرف: زيد محمد مبارك المهداوي | موسى جاسم محمد الحميش
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: صلاح الدين
الصفحات الاولى:
المستخلص: صممت الدراسة الحالية لمعرفة تاثير الاجهاد التاكسدي لدى المرضى المصابين ببعض امراض القلب عن طريق اجراء بعض الاختبارات والقياسات كالنسبة المئوية لحجم كريات الدم المرصوصة (PCV) وتركيز خضاب الدم (Hb)، وبعض الانزيمات مثل : تركيز انزيم ناقل امين الاسبارتيت (AST | The current study was designed to determine the effect of oxidative stress in patients with the certain heart diseases through some tests and measurements as the Packed Cells Volume Percentage (PCV) and hemoglobin concentration (Hb), and some enzymes as : the Concentration of Enzyme Aspartate Aminotransferase (AST), Creatine Phospho Kinase (CPK), Total Lactate Dehydrogenase (LDH), and Lactate Dehydrogenase For Heart Muscle, and some of antioxidants and oxidation factors as : Glutathione (GSH), and Malondialdehyde (MDA) respectively, and lipid profile that include : the Concentration of Total Cholesterol, Triglycerides (TG), High Density Lipoprotein of Cholesterol (HDL - C), Low Density Lipoprotein of Cholesterol (LDL - C), and Very Low Density Lipoprotein of Cholesterol (VLDL - C), and some other biochemical criteria as : the Concentration of Glucose, Total Protein, Albumin, Globulin, Urea, and Creatinine. It was chosen (95) blood sample back to the (80 patients and 15 healthy people), the samples were divided into four groups : The first group : included patients with Myocardial Infarction and they was (40) patients (males and females), age ranged between (35 - 89) years old.The second group : included patients with Angina Pectoris and they was (20) patients (males and females), age ranged between (32 - 85) years old.The third group : included patients with Heart Failure and they was (20) patients (males and females), age ranged between (39 - 89) years old.The control group : included (15) people (males and females), age ranged between (30 - 57) years old. The current study showed the following results : A. A significant decrease in the level (p <0.05) in : 1 - The Packed Cells Volume Percentage (PCV) and the concentration of Hemoglobin (Hb) in the heart failure group within the group of (males+females) and females gender.2 - Concentration of Glutathione (GSH) in the three pathological groups within the groups of males and females and (males+females) and age groups (60 years old and above).3 - Concentration of (HDL - C) in the three pathological groups for males and (males+females).4 - Concentration of Total protein in the myocardial infarction group for males and in the heart failure group for females and these two groups within the group of (males+females) and smokers.5 - Concentration of Albumin in the myocardial infarction and heart failure groups for males and (males+females).6 - Concentration of Globulin in the heart failure group for females and (males+females).B. A Significant increase in the level (p <0.05) in : 1 - Concentration of Enzyme Aspartate Aminotransferase (AST) in the angina pectoris group for males and the heart failure group for females and these two groups within the group of (males+females), males gender, diabetes and smokers.2 - Concentration of Enzyme Creatine Phospho Kinase (CPK) in patients with myocardial infarction for males and female and (males+females), males gender, age group (80 - 89 years old), people with high blood pressure, diabetes and smokers.3 - Concentration of Enzyme Total Lactate Dehydrogenase (LDH) and Lactate Dehydrogenase For Heart Muscle in the myocardial infarction group for males and females and (males+females), males gender, age group (50 - 59 years old), diabetes and smokers.4 - Concentration of Malondialdehyde (MDA) in the heart failure group for males and females and (males+females) and females gender.5 - Concentration of Total Cholesterol in a group of angina pectoris for males and females and (males+females).6 - Concentration of Triglycerides (TG) in the myocardial infarction group for females and (males+females), males gender, diabetes and smokers.7 - Concentration of (VLDL - C) in the myocardial infarction group for males and females and (males+females), males gender, diabetes and smokers.8 - Concentration of (LDL - C) in the angina pectoris and heart failure groups for males and females and (males+females).9 - Concentration of Glucose in the heart failure group for males and myocardial infarction group for females and these two groups within the group of (males+females), males gender, age groups (50 - 79 years old), diabetes and smokers.10 - Concentration of Urea in the myocardial infarction and heart failure groups for males and females and (males+females) and age groups (from 60 - 79 years old).11 - Concentration of Creatinine in a myocardial infarction group for males and females and (males+females) and age group (70 - 79 years old).

تاثير الدقائق النانوية للفضة في بعض الجوانب التركيبية والوظيفية للجهاز التناسلي الذكري للفئران == Effect of Silver Nanoparticles On Some Structural And Functional Parameters of Male Reproductive System of Mice

اسم المؤلف: امير محمد جعفر علي حسين البلداوي
اسم المشرف: محمد ناجي طه
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الفسلجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: تطرقت هذه الدراسة الى معرفة تاثير الدقائق النانوية للفضة (Ag NPs) في بعض الجوانب التركيبية والوظيفية للجهاز التناسلي الذكري للفئران. جرعت الحيوانات فمويا بالـ Ag NPs بجرعة 5 ملغم/مل وللمدد 5, 10, 15 يوما وفي اليوم التالي لانتهاء كل مدة معاملة تمت التضحية | This study touched to know the effect of silver nanoparticles (Ag NPs) on some organs of the reproductive system and the testosterone hormone in male albino mice. animals were orally dosed with 200 mg/kg of Ag NPs for 5, 10, 15 days and the next day to the end of each period of treatment has animal sacrificed and autopsy after taking their weights before and after the treatment for the comparison between them and then took the testes and epididymis (head and tail) from them after measuring their weights. This study included the study of changes in the weight of animals and organs, sperm characters in both the testes and epididymis, the percentage of cells generating sperm, the number of Leydig cells and the diameters of these cell nuclei, changes in the seminiferous tubules in the testes and changes in epididymis tubule and histopathological changes as well as changes in testosterone level. The results of the animals weights before treatment showed a significant decrease (P<0.05) compared to the weights of the same animals after treatment, while the results of this study after comparing with the control group showed a significant decrease (P<0.05) in the weights of testes and tunica albuginea for the periods of 5, 10 and 15 days. The epididymis showed a significant decrease (P<0.05) in the average weights of the epididymis head for the three periods while the average weights of the epididymis tail showed a significant decrease (P<0.05) for the periods of 10 and 15 days. The vitality and the concentration of the sperms showed a significant decrease (P<0.05) in their percentages for the three periods. All the results above showed a significant decrease (P<0.05) when they compared between the three periods of the treatment. While the sperm abnormalities showed a significant increase (P<0.05) in their percentage in each of the testes and epidydimes for the periods 5, 10, 15 days with a significant increase (P<0.05) in these abnormalities when they compared between the three periods of the treatment. The histological results of this study after comparing to the control group revealed histopathological changes in the testes and the epididymis (head and tail). The percentage of spermatogenesis showed a significant decrease (P<0.05) in the number of spermatogonia, primary spermatocyte and sperms when compared to the control group with a significant decrease in the number of these cells when compared the three treatment periods with each other. While the number of secondary spermatocyte and spermatids was significantly increased (P<0.05) when compared with the control, with a significant increase in the number of these cells when comparing the three periods of treatment. Whereas the results of Leydig cells after comparing to the control group showed a significant decrease (P<0.05) in the number and the diameter of the cell nucleus. The results of this study also showed a significant decrease (P<0.05) in the thickness and the diameter of the seminiferous tubules of the testes and the thickness, The epithelium height of the epididymis (head and tail) when compared to the control group with significantly decrease in the thickness and diameter of the seminiferous tubules of the testes and the thickness and epithelium height of the epididymis (head and tail) when they compared between the three treatment periods. The results of this study when compared to the control group showed a significant decrease (P<0.05) in the level of the testosterone hormone with significantly increase (P<0.05) in the testosterone level when compared between the periods of 5, 10, 15 days. From these findings it is concluded that the AgNps crossed the blood - testis barrier and caused many changes to the testicular tissues and decreased the level of the testosterone. This may affect the fertility of mice.

تاثير تربية الاسماك بالاقفاص الموضوعة في نهر دجلة المار بمدينة بغداد في مجتمع الهائمات القشرية == The Impact of Fish Farming By Cages Placed In The Tigris River At Baghdad City On The Community of Crustacean Zooplankton

اسم المؤلف: حارث قاسم مهدي
اسم المشرف: صباح فرج عبد الاحد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - اللافقريات
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: ان الاثار البيئية لزراعة الاسماك في اقفاص التربية في العراق لم تتم دراستها بصورة جيدة،علما ان هذا النشاط يمارس على نحو متزايد وهناك حاجة كبيرة لدراسة الاثار المترتبة على ذلك.اجريت الدراسة الحالية على مواقع اقفاص تربية الاسماك في نهر دجلة شمال بغداد للتعر | The present study took place on fish farming cages located in the Tigris River in the north Baghdad, to in order to study the effects of high fish densities on zooplankton communies in addition to study of some physical and chemical factors.The environmental effects of fish farming in cages in Iraq have not been studied well, knowing that this activity is practiced increasingly and there was a significant need to study the implications of this important mater.The aim of this study was to investigate the effects of carpfish farming cages on the density of zooplankton in the Tigris River, The samples were taken twiece a month form the three stations for six months from January to June 2014 in three selected stations in the river stream : the first place was Al - Rashidiya station which located in Al - Rashidiya area (Kadhim Al - Ali), 5 km from tourist Baghdad island and the second station was Algriaat 1 which located in Algriaat area in Rusafa side of Baghdad city corresponding to the Kadhimiya near the bridge of riverain pedestrian (Al - Duab bridge) connecting between Al - Rashidiya, while the Al - Kadhimiya and the third station was Algriaat 2 which located in Algria’at zone corresponding to the area of Kadhimiya.The study included the identification studying the density of three grops of zooplankton which were Calanoida, Cyclopoida and Cladocera in addition to the measurement of some physical and chemical characteristics of river water and its effect on the three zooplankton community according to their importance, which included velocity of water current, water temperature electrical Conductivity and Salinity, pH, dissolved oxygen(DO), biological oxygen demand (BOD), phosphate concentration and nitrate concentration. The results of the present study showed that the density of Cladocera. Was lower than the densities of Calanoida & Cyclopida in the three station. on the other hand the current study showed that the densities of both Calanoida and Cyclopoida were oscillatory during the months of th study between highest and lowest, without in statistically significant differences, but the highest densities in June, and this study shows that the highest densities of the three groups of zooplankton were recorded in June. The present study showed a negative effect of the high density of the fish in the cages on the density rate of planktonic crustacea by reducing the numbers and the density of planktonic crustacea community inside cages. Compared with planktonic crustacea community outside the cages and with those within 100 meters far from cages, the differences were statisticaly singnficance.On the other hand regarding to the results of the physical and chemical factors it was found a highest that the velocity of water current rate was 82.50 cm/s in Algria’at 2, while the lowest was 43.33 cm/s in Al - Rashidiya station.Water temperatures ranged between 9C° in Algria’at 2 to 32C° in Al - Rashidiya station during the study period.The highest rate of electrical conductivity was 1032.00 ?S/cm in Algria’at 1, while the lowest was 765.25 ?S/cm in Al - Rashidiya station, while the highest rate of the salinity concentration in the fish cages was 0.66 mg/L in Algria’at 1 and the lowest concentration of salinity was in Al - Rashidiya 0.48 mg/L.pH values ranged between 7.20 in Algria’at 2 to 8.05 in Algria’at 1 in March for both stations, whil the concentration of dissolved Oxygen in water concentration ranged between 5.95 mg/l in Al - Rashidiya to 7.70 in Al - Rashidiya and Algriaat 1. and the Biological Oxygen Demand ranged was between 4.11 mg/l in Algriaat 1 to 6.50 mg/L in Al - Rashidiya station during February for both stations. It was also found that the lowest concentration of phosphate was 0.016 ml/l in Algriaat 2 in april while the highest concentration was 0.075 mg/l algreaat2 in march Also it was found that the lowest cocentration of nitrate 0.82 mg/l was recorded in al griaat 2 in fabruart while the highest conceneation 1.90 mg/l was recorded in Al Rashidya in june.All the results of the physical and chemical factors founded were within normal allowable environmentally boundaries and which have no effect on the aquatic planktonic crustacea community.

التشخيص الجزيئي للخمائر والاعفان المرافقة لمخلفات الطيور وتقييم كفاءة بعض المستخلصات النباتية ضد خميرة Cryptococcus == Molecular Identification of Molds And Yeast Associated To Birds Wast And Evalution Efficiency of Some Plant Extract Against Cryptococcus

اسم المؤلف: رسل عصام علي الظاهر
اسم المشرف: زيدان خليف عمران المعموري
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: تضمنت الدراسة الحالية عزل وتشخيص الاعفان والخمائر المرافقة لمخلفات الطيور باستخدام بعض التقنيات المظهرية والجزيئية. كما تم اختبار فعالية المستخلصات المائية لنبات وعرك لوية والقرنفل والبابونج في تثبيط نمو خميرة Cryptococcus neoformans.تم جمع 190 عينة من م | The present study aimed to isolation and diagnosis the molds and yeast from birds extract by use some of phenotype and molecular techinces. As well as the aqeous plant extracts of clove and Germin Chamamilla and east indian scrow tree.to inhibition Cryptococcus neoformans growth there is about 191 isolate was been collected from Babil and Karbala included 30 isolate (10 isolate hand printing and 10 isolate from ambiend air of bird brought zone, and 10 isolate from cough of men they are dealing with birds in pitry dish containing SDA media. The other 161 isolate was been collected from the wet and dry bird extract from the different environment.Culture method was used in the diagnosis as CHROMagar media to diagnosis Candida spp. Also used the haemolysis test of Macrorhabdus ornithogaster yeast and phospholipase and Urease test of Cryptococcus neoformans yeast.And used the molecular methods as DNA extraction and used PCR technice by use universal and specific primers.Results of analysis isolation and diagnosis showed that the maximum of present appearance was for Candida spp. 45.34% and molds Rhizopus sp. appearance percent 27.32% and frequency percent 28.21% and 3.65% consequently M. ornithogaster appearance percent 4.96% and frequency percent 35.77% while the other molds and yeast have appearance percent between 0.62 - 19.25% and frequency percent between 0.07 - 19.75% and the result of CHROMagar for Candida gives different color C.albicans was green color and C.krusei was pinki color.Cryptococcus showed apositive result in phospholipase and Urease test. haemolysis test of Macrorhabdus yeast was positive the hot aqeous plant extracts of clove and Germin Chamamilla and east indian scrow tree have the activity to inhibit the growth of Cryptococcus neoformans, but the more activity was east indian scrow tree with concentration 4%.The pair primer CAP60 succesful to diagnose Cryptococcus neoformans It was 603bp and primer AGY1\Sm2 successful to diagnose Macrorhabdus yeast

دراسـة مظهرية وتشريحيـة مقارنة للاجناس Sophora L. وTaverniera DC. وSecurigera DC. (Leguminosae) في العراق == Morphological And Anatomical Study of Genera Sophora L., Taverniera Dc. And Securigera Dc.(Leguminosae) In Iraq

اسم المؤلف: زهراء قاسم عبد محسن الكلابي
اسم المشرف: ازهار عبد الامير سوسة
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: تضمن البحث الحالي دراسة مظهرية وتشريحية لثلاث اجناس للعائلة البقولية Leguminosae في العراق وهي Securigera DC. وSophora L. وTaverniera DC.، ودرس نوع واحد من الجنسين الاول.securidaca S والثالث T.nummularia ونوعين من الجنس الثاني So.japonica وSo.gibbosa.تم | The present research included Morphological and Anatomical study for three genera Securigera DC. , Sophora L. and Taverniera DC. belong to the family Leguminosae in Iraq. Four species it was taking one species of the first genus S.securidaca and third genus T.nummularia , two species from the second genera So.japonica, So.gibbosa. The study of Qualitative and quantitative characters of vegetative and reproductive parts were also study and discuss The variation in These qualities. Morphological traits were compared in terms qualities of characters of Habit and Duration , stems, leaves, stipules, inflorescences , bracts and parts floral , fruit and indumentum. Species were separated and placed in groups depending on the obvious differences and similarities these qualities as some of morphological characteristics such as the shape of Leaves, if possible to isolate species T.nummularia it have Unifoliolate Leaves and the outer species have Imparipinnate and the rest of stipules shape in isolate species S.securidaca if was a shape Foliar and other species was Spiny and other qualities. other variations have been discussed with in the qualities. And it became clear of Habit and Duration , stems, leaves, stipules, bracts and parts floral, fruit were of considerable taxonomic value. The study also involved anatomical characters of different organs particularly the leaf epidermis, indumentum, cross section of the leaves, petioles and stems also venation.as some Anatomical characters like species S.securidaca for the rest of the species it Undulate wall clearly been under review also benefit from cross section of the stems as possible to isolate species S.securidaca it was Hollow and empty Pith from the cinter either species was Solid if occupies the Pith status. show that many of anatomical characteristics specially the stem, leaf and also the shape of the walls of epidermal cells and some qualities indumentum taxonomic importance were assessed. Taxonomic treatment has been carried out for all genera and their species and a key was designed for them. with description for each genus and species in this study.

التاثير القاتل لمستخلصات نبات اليوكالبتوس Eucalyptus camaldulensis في يرقات بعض انواع البعوض (Diptera : Culicidae) في التجمعات المائية لمزارع القمح والشعير في ضواحي مدينة الكوت - العراق == Extracts Killer Effect of Eucalyptus Camaldulensis Plant On Larvae of Some Mosquito Species (Diptera : Culicidae) In Aquatic Pools of Wheat And Barley Farms In Suburbs of Kut / Iraq

اسم المؤلف: زينب محمد عباس
اسم المشرف: جميل سعد السراي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: واسط
الصفحات الاولى:
المستخلص: شملت الدراسة جمع عينات من يرقات الطور الثالث والرابع لبعض انواع البعوض من الجداول المائية لمزارع القمح والشعيرفي مدينة الكوت - واسط / العراق خلال الموسم الزراعي الممتد من تشرين الثاني 2013 ولغاية نيسان 2014 ومن ثم تقييم فاعلية المستخلص المائي والكحولي لاو | The present study was established to evaluate the effectiveness of the water and alcohol extracts of Eucalyptus camaldulensis Dehnhardt Leaves on the killing of the 3rd - 4th instar larvae of some mosquito species collected from Wheat and Barley fields in AL - Kut city, Wasit province / Iraq, during the period of six months (November 2013 - May 2014). This study has been demonstrated the following results : The study showed the presence of three types in the study aquatic environment distributed on gender, type I return to the genus Aedes, a type Aedes caspius pallas and appeared during months November 2013 to February 2014. The types other two Faaudan to genus Culex two type Culex tritaeniorhynchus Giles and Culex pipines L has appeared during March and April 2014, respectively, of a monthly period extending from from November 2013 to the end of the study in April 2014. The results of the study showed the water and alcohol extract leaves the plant E. camaldulensis kill larvae in the third and fourth instar of the three types of mosquitoes effect. The following : - The alcohol extract of Eucalyptus camaldulensis leaves was more effective on the mortality of mosquito larvae than water extract after 24h following treatment. In which the (10000 ppm) at the highest concentration of both extracts (alcohol and water) showed the highest response. The mortality rate of larvae Ae.caspius of alcohol and water extracts were (100, 90) % respectively. As well as the alcoholic LC50 and LC90 that were given to same species, were calculated and the results were (8, 2700 ppm) respectively. Whilst the water extract at the same lethal concentrations findings were (2500, 9900 ppm) respectively. - The mortality rate of larvae Cx. tritaeniorhynchus of alcohol and water extracts were (73, 66) % respectively. As well as the alcoholic LC50 and LC90 that were given to same species, were calculated and the results were (6400, 12500 ppm) respectively. Whilst the water extract at the same lethal concentrations findings were (5600, 11400 ppm) respectively. - The mortality rate of larvae Cx. pipines of alcohol and water extracts were (43, 3.3) % respectively. As well as the alcoholic LC50 and LC90 that were given to same species, were calculated and the results were (16100, 22300ppm) respectively. Whilst the water extract at the same lethal concentrations findings were (zero, zero) respectively.

تاثير طريقة الزراعة بالشتل لصنفين من الذرة الصفراء Zea mays L. الربيعية تحت نظام الري بالتنقيط == Effect of Transplanting Method of Two Maize Cultivars Zea Mays L. Under Drip Irrigation System

اسم المؤلف: نضال ياسر عباس الغركان
اسم المشرف: وسام مالك داود
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: A field experiment was carried out at the Experimental Fields of Department of Field Crops Sciences - Faculty of Agriculture - Diyala University during 2014 within the project of the development of maize for spring season in Iraq to study the response of two varieties of maize grown in spring season to transplanting technique under drip irrigation system. Maize seed of varieties Broujen and Cadiz were planted in dates 15/ 1, 15/ 2 and 15/ 3 in Polyethylene bags 8 cm diameter and 18 cm depth in the greenhouse. Seedlings were transferred on 15/1 and 15/2 to the field in 15/3. While the varieties of maize planted inside a greenhouse in 15/ 3 when plants were 4 to 5 leaves transferred to the permanent field. It was in 5 / 4, after 21 days from planting. The experiment was applied according to the Split Plots Design with three replications. Each replicate was divided into two main plots (Broujen and Cadiz variety). Each main plot was divided into four secondary plots which were treatments of methods of agriculture (method of transplanting technique in the greenhouse which is seedlings 15/1, seedlings 15/2, seedlings 15/3 and traditional agriculture method in the permanent field in 15/3). Results have shown that seedlings dates 15/1 and 15/2 led to the events of a significant increase in the mean of plant height, number of leaves, leaf area and leaf area index. they reduce in the vegetative growth period and increase in the weight and length of ear, the number of fertilized grain in it, grain yield, biological yield, harvest index and percentage of oil. Varieties showed significant effects on the growth and yield characters. Broujen variety has achieved increase in mean of plant height, stem diameter and reduce in the vegetative growth period and increase in the biological yield. The interaction between the methods of agriculture (method of transplanting technique and traditional agriculture method) and varieties have shown significant effect in stem diameter (before flowering and at the maturity), green leaves number, dead leaves number, leaf area, leaf area index, vegetative growth period and flowering growth period. Therefore, The interaction between the methods of agriculture and varieties have shown significant effect in ear weight, grain weight, cob weight, fertilized grain number, aborted grain number, biological yield, grain yield, harvest index and percentage of oil and protein.

تحضير مركبين نانويين هجينين من الكلورهكسدين وحامض التانيك وتحديد فعلهما المضاد للاحياء المجهرية المعزولة من مرضى الحروق في محافظة كربلاء المقدسة == Preparation of Two Nanohybrid Compounds From Chlorhexidine And Tannic Acid And Determination Their Antimicrobial Activities Against Microorganisms Isolated From Burns Patients In Sacred Karbala Province

اسم المؤلف: نورس مجيد حميد الخفاجي
اسم المشرف: علي عبد الكاظم الغانمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: تضمنت هذه الدراسة عزل وتشخيص اهم انواع البكتريا المسببة لاخماج الحروق كما تضمنت ايضا تحضير وتشخيص مركبين نانويين هجينين احدهما من المطهر كلورهكسدين والاخر من حامض التانيك. تم تقييم الفعالية التثبيطية للمطهر النانوي المحضر اعلاه ضد البكتريا المعزولة في هذه | This study included the isolation and diagnosis of the most important species of bacteria responsible for the infection in burns. It also included the preparation and detection of two hybrid nano compounds, one of them from the disinfectant chlorhexidine and the other from the tannic acid. The inhibitory activity of the nano disinfectant was assessed against the isolated bacteria in this study also, the antioxidant activity of the hybrid nano tannic acid was identified and the results showed the following : 1 - As a total 32 samples were taken from burned patients hospitalized in the burning ward Imam Al - Hussien medical city in Sacred Karbalaa governate. It had been obtained 8 isolates (34.78) % from Pseudomonas spp., 7 of them were Pseudomonas aeruginosa and one was P. oryzihabitans , 4 isolates (17.39) % were Acinetobacter baumannii, 4 isolates (17.39) % were Klebsiella pneumoniae, and one isolate (4.34) % of Proteus mirabilis, Escherichia coli, Serratia liquefaciens and Chryzeobacterium meningosepticum and two isolates (8.69) % of Staphylococcus aureus in addition to one isolate (4.43) % from Candida guilliermondii yeast.2 - Chlorhexidine disinfectant and tannic acid were used in preparation of the two hybrid nano compounds after intercalating each of them on layers of double hydroxide (Mg/Al - LDH). FT - IR spectrum studies has shown the appearance of specific chemical groups and the disappearance of others while the spectrum of x - ray(XRD) has shown the appearance of new levels of diffraction in the spectrum of the hybrid nano disinfectant Mg/Al - CHX - LDH and the spectrum of the hybrid nano tannic acid Mg/Al - TA - LDH as compared to the spectrum of the Mg/Al - LDH, which indicate that the two prepared compounds under study is a nano hybrid compounds. Results of the scanning electronic microscope has shown, the change in the shape of the surface of the nanohybrid compounds that prepared above as compared to the layer of double hydroxide. It turns out from the results of the examination with the use of Atomic Force Microscope (AFM), that the diameter of the particles of hybrid nano disinfectant and hybrid nano tannic acid were (121.66 and 77.70) nm, respectively. 3 - The inhibitory activity of the hybrid nano disinfectant Mg/Al - CHX - LDH and the free disinfectant (Free CHX) has been studied and the results revealed followings : a. The hybrid nano disinfectant has the highest inhibitory activity against the two bacterial isolates P. aeruginosa (11 and 23 - A) with an inhibition diameter of 24 mm for each, while the inhibition diameter for the free disinfectant was 23.5 mm against the isolate P. aeruginosa 4 - C.b. The hybrid nano disinfectant showed the highest inhibitory activity against the two isolates A. baumannii (16 - A and 31 - A) with an inhibition diameter of 18 mm for each, while the highest inhibition activity for the free disinfectant was against the isolate A. baumannii 22 - B with an inhibition diameter of 19.5 mm.c. The hybrid nano disinfectant showed the highest inhibitory activity against the isolate K. pneumoniae (16 - B) with an inhibition diameter of 24.5 mm, while the highest inhibition activity for the free disinfectant was against the isolate K. pneumoniae 15 - B with an inhibition diameter of 24.5 mm also.d. The highest inhibition activity for the hybrid nanoic disinfectant against each of bacteria P. mirabilis and E. coli with an inhibition diameter of 23.5 mm, while the highest inhibitory activity for the free disinfectant was against the bacteria E. coli with an inhibition diameter of 24.5 mm, while the bacteria C. meningosepticum was the least affected among the other types of the isolated bacteria with an inhibition diameter of 8 mm and it was resistance to the hybrid nano disinfectant.e. The highest inhibition activity for the hybrid nano chlorhexidine was against the isolate S. aureus 21 with an inhibition diameter of 28.5 mm, while the highest inhibition activity for the free chlorhexidine was against the isolate S. aureus 22 - A with aninhibition diameter of 31.5 mm.f. The inhibitory activity of both the hybrid nano and the free disinfectant against C. guilliermondii yeast was closely to each other with an inhibition diameter of (28 and 29) mm, respectively.4. The study of antioxidant activity confirmed the following : a. Using free radical scavenging method, the IC50(Half maximal inhibitory concentration) value against ABTS free radical were at 10 µg /ml for free tannic acid while it was at 125 µg /ml for the hybrid nano tannic acid Mg/Al - TA - LDH.b. Measurement of ferric ion reducing power revealed that free tannic acid had reducing power greater than that of the hybrid nano tannic acid

التوصيف الجزيئي لبعض مسببات الاخماج الجلدية في محافظة الديوانية وتاثير جزيئات الفضة النانوية في نموها == Molecular Characterization of Some Dermatophytic Fungi In Al - Diwania Province And The Effect of Silver Nano Particles On Their Growth

اسم المؤلف: نور جاسم محمد
اسم المشرف: نيران عبيد جاسم
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة للتحري عن الفطريات المسببة للامراض الجلدية في الانسان حيث تم جمع 100عينة من الاشخاص المشكوك باصابتهم بالفطريات الجلدية من المرضى المراجعين لمستشفى الديوانية التعليمي من بداية تشرين الاول 2013 وحتى نهاية مايس 2014 وكانت هذه النماذج ماخوذة | This study was conducted to investigate the fungi that causes skin diseases of human. One hundred samples were collected from people who suspected to infect with fungal skin disease from patients coming to Al - Diwaniya teaching hospital from beginning of October 2013 till the end of May 2014. These samples from (skin, hair and nail). All samples that taken from patient were subjected to direct examinations by using wet touch of (KOH 10%) solution. At the same time the samples were grew on sabroud media to determine the type of fungal skin diseases of human. The direct examination shows positive results of 72 samples which represent 72% while for laboratory growth results, its shows 65 samples with positive results 65%. There are three types of Trichophyton fungus had been isolated which are T.rubrum 28 isolates, T.mentagrophytes 16 isolates and T.schoenleinii 4 isolates and there was one type of genus Microsporum which is M.canis 24 isolates. The results show that Tinea corporis infection is the common and its more among females than males where it is 26.39% and 13.89% respectively, then Tinea capitis which is 19.4% in males which is higher than in females which represents 13.89% while Tinea unguium was in females more than males which was in females 16.67% while in males 9.72%. The percentage of fungal skin infection was differ according to the location where the number of infections in the rural area for all types of Tinea were more than the number of infection in the urban area where the number of infected people with Tinea corporis was 18 which represent 25% while in the urban the number was 11 which represent 18.28% while the number of infected people with Tinea capitis in the rural area were 16 which represent 22.22% and the infected in the urban were 8 which represent 11.11%. While Tinea unguium the number of infected people in the rural 10 which represent 13.89% and in the urban 9 people which represent 12.5%. The diagnosis of isolated fungi depended on phenotype of fungal growth and microscopic examination, in addition to special tests for diagnosing skin fungal infections. Also the PCR was used for diagnosis and the work had been completed by using genotypic tree for fine diagnosis and detection the fungal species. The result were send to the genes bank site to be sure about the fungus type through comparison with the registered fungi in this site and by using MEGA6 program for genetic tree analysis of type (unweighted pair group method with arithmetic mean). The results of analysis shows clear converge of local Trichophyton spp. Isolates with Trichophyton rubrum in comparison with other types that appears in the genetic tree analysis while Microsporium diagnosed through genetic tree analysis of type (up GMA tree) and the result of analysis shows clear converge of local Microsporium spp. Isolates with Microsporium canis compared with other types of genetic tree analysis. The effect of silver nanoparticles that is used against growth of T.rubrum, T.mentagrophytes and M.canis. The results show that all the concentrations used were effective in stopping of growth of T.mentagrophytes when deal with the standard measure when dealt with concentration of 4 mg/l the more effective in stopping of radiating growth of the fungus. Regarding the fungus T.rubrum the concentrations of 4 mg/l more effective in stopping of growth while there was no difference for 1mg/liter concentration. Regarding the fungus M.canis, the mentioned concentrations had large effect on fungal colonization growth compared with the standard and this fungus more than other fungi affected by nano silver ions

تاثير تراكيز مختلفة من الجبرلين والسماد النتروجيني في نمو وحاصل والمادة الفعالة لصنفين من نبات الكرفس Apium graveolens L. == Effect of Use Different Concentration of Gibberellic Acid And Nitrogen Fertilizer On The Growth, Yield And Active Substance For Two Cultivars of Celery

اسم المؤلف: ماهر جاسم محمد الاسدي
اسم المشرف: انتصار حسين مهدي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: النبات
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: نفذت التجربة في الموسم الشتوي لعام (2013 - 2014) م في اصص بلاستيكية بقياس 15×16 سم في محافظة ذي قار, لمعرفة تاثير الرش بحامض الجبرلين ومستوى السماد النتروجيني والتداخل بينهما في النمو ومحتوى الثمار من الزيت الطيار والمادة الفعالة لصنفين من نبات الكرفس Api | The experiment was conducted in the winter season of the year (2013 - 2014) A.C. in a home garden in the governorate of Dhi Qar, to find out the effect of spraying Gibberellic acid and level of nitrogen fertilizer and the interference between them in the growth and content of the fruits of the essential oil and active substances for two cultivars of Celery (Apium graveolens L.).The design of The experiment was Randomized Complete Blocks Design (RCBD) with three replicates in factorial arrangement for three factors (2×4×4 the first factor includes two types of Celery cultivars (local and imported), the second factor includes four concentrations of Gibberellic acid (0, 50, 100 and 150) mg.L - 1, the third one includes four levels of nitrogen fertilizer (0, 10, 20 and 30) kg.dunam - 1. used Revised Least Significant Difference (RLSD) at probability level of (0.05) to compared the averages. The results showed the following : 1 - The local cultivars was significantly Outweighed in content of chlorophyll a, b and total chlorophyll and the percentage of essential oil in plant seeds. imported cultivars was significantly Outweighed on the local cultivars in other characters. 2 - The applying of Gibberellic acid caused a significant increase in all traits, the highest percentage increasing of the Characteristics at a concentration of Gibberellic acid (150) mg.L - 1 for most traits.3 - The nitrogen fertilizer levels (30)) kg.dunam - 1 were used gives significant increase in all of studied traits of the plant except for the percentage of phosphorus, where the increase did not reach to the significant level.4 - The interference between cultivars of plant and Gibberellic acid showed a significant difference in most of the studied traits except the Characteristics stem diameter, number of branches, flowering inflorescences, the wet and dry weights for shoot and root increase in significantly. the treatment of (150)) mg.L - 1 with a imported cultivar was Outweighed significantly in most vegetative growth Characteristics.5 - Binary interference between the plant cultivars and nitrogen fertilizer showed a significant effect for most of the characteristics, which reached the highest increase significantly treatment (30) Kg.dunm - 1, nitrogen of the imported cultivars. 6 - The binary interference between Gibberellic acid and nitrogen fertilizer caused significant effect in all traits except number of branches. Outperforming combination plants (150) mg.L - 1, Gibberellin and (30) Kgm.dunm - 1, significantly nitrogen fertilizer in most vegetative growth Characteristics and floral characteristics and the percentage of oil and active substances and yields the plant from seeds and t1000 seed weight.7 - The triple interference between study factors significantly affected the most qualities, where the given combination (150) mg.L - 1, Gibberellin and (30) Kg.dunm - 1, fertilizer nitrogen in the imported cultivars highest significant increase in most vegetative growth Characteristics and yields the plant from seeds.

تاثير نقص البروتين في بعض التغيرات النسجية والسريرية والوظيفية في ذكور الفئران البيض == The Effect of Protein Deficiency In Some Clinical, Histological And Physiological Changes In Male White Mice

اسم المؤلف: نسم عماد دايم الفؤادي
اسم المشرف: فاضل فرهود مكي الجبوري | هاشم محمد عبد الكريم العلاق
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: علوم الحياة
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بابل
الصفحات الاولى:
المستخلص: The present study is designed to know the effect of protein deficiency on mice males through study the clinical histological changes of some organs including (liver, kidney, stomach and testes).In addition of study the blood and biochemical parameters, where they were sheltering animals in the animal house of the branch of physiology / College of Veterinary Medicine / University of AL - Qadisiya and the number was 90, all of the mice are males and age (28 - 30) days kept for two weeks before starting the experiment for the purpose of acclimatization was given during this period, diet control, then the experiment began between 01/05/2014 until 19/2 / 2014, and at weights ranging from start racing experience between 17 - 18 g, all in a period of growth.These animals were divided into three groups, each group contains 30 mice, first group (control) was give 18% total protein contains animal protein 7%, while the second group was given 13% total protein contain 3.5% animal protein, and the third group was given 10 % total protein without any animal protein for different period as follow (15, 30, 45)day.Experiment takes study of changes in body weight of animals and some selected organs also study histological changes for studied organs, also physiological blood parameters such as PCV, Hb, GCS and TSP.Results showed significant decrease (p<0.05) in weight body rate of animals, also in the rate of selected organs means compared with control group. The second treatment for 45 days most significant in decreased of body weight and organs weight if it compared with first treatment in all periodsHistological procedures showed that clinical and histological changes are graduated by the severe of protein deficiency and period of time. The histology investigation for liver showed that congestion of blood vessels accompanied with dilation of central vein and portal vein, and necrosis of the hepatic cells and destroyed the general total structure of liver. The section of kidney showed that necrosis and destroyed in renal tubules and epithelial cells in the lumen of tubules and bleeding in renal tubules and clear shrinkage in glomerulus. There are histological changes in stomach by necrosis and desquamation for gastric cells in both period (30, 45) days, while there are no histological clinical changes in both doses in period (15 days) for exposure. Histological sections of testes showed induction inhibition of spermatogenesis and desquamation of germ cells and congestion in the interstitial tissue and dilation of lumen of seminiferous tubules in both periods (30, 45) days, while there is no any changes in both first and second treatment in period of (15)days.In contrast, the hematological parameters showed that the concentration of Hb, PCV significant decrease (p<0.05) in blood of treatment animals which correlated with protein deficiency compared with control group, and the second treatment for (45) days showed the most significant in the parameters compared with the first treatment and for all periods.Biochemical tests obtain significant decrease (p<0.05) in blood glucose level in both treatment first and second in first period. Also the results showed that no significant increase in first treatment in both periods (30, 45) days compared with control group, while there is significant increase in the level of blood glucose in the second treatment in both periods (30, 45) days compared with the control group. Also did not score a significant decrease (P <0.05) in the total serum protein in thefirst treatment in the first period (15) days, compared with the control group, and found a significant decrease (P <0.05) in the first treatment in the two periods (30, 45) days compared with the control group, while found a significant decrease (P <0.05) in the total serum protein in the second treatment period (15, 30, 45) days compared with the control group.It can be conclusion that the exposure for protein deficiency due to clear clinical histological changes for studied organs and some changes in some hematological and biochemical parameters in white mice which treated with protein deficiency which it causes these symptoms

تاثير المستخلص المائي الخام لنبات الزنجبيل Roscoe officinale Zingiber على بعض الجوانب الدموية والتناسلية في الفئران الحوامل == Effect of Crude Aqueous Extract of Zingiber Officinale Roscoe On Some Hematological And Reproductive Aspects In Pregnant Mice

اسم المؤلف: رغد خالد موفق مسلم
اسم المشرف: محمد عبد الهادي غالي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة للتعرف على مدى تاثير المستخلص المائي الخام للزنجبيل Zingiber officinale Roscoe (الطازج، اوالجاف) على الفئران الحوامل المجرعة عن طريق الفم وبالتراكيز (284، 568، 1136، 1420 ملغم/ كغم) اثناء مراحل الحمل المختلفة (المبكرة والوسطية والمتاخرة | This study was designed to investigate the effect of the crude aqueous extract of ginger (Zingiber officinale Roscoe) (fresh or dry) on pregnant mice orally administered at doses (284, 568, 1136, 1420 mg/kg) during different durations of pregnancy (early, intermediate, and final) for a period of 7 days for each duration. The study showed that there was no significant difference in the average weights of the animals at (P < 0.05) after treatment with (dry or fresh) extract at doses (284, 568, 1136 or 1420 mg/kg) comparing with the control group.The parameters of blood in the early duration of pregnancy showed a significant decrease in the average of hemoglobin (Hb) at (P < 0.05) after treatment with (fresh or dry) extract at the doses (568, 1136, 1420 mg/ kg), while the average of packed cell volume (PCV), demonstrated a significant decrease after treatment with fresh extract at doses (284, 568, 1136, 1420 mg/kg), and at (568, 1136, 1420 mg/kg) after treatment with dry extract at (P < 0.05) : The average of red blood cells (R.B.C.) after treatment with (fresh or dry) ginger extract, at (P < 0.05) showed a significant decrease at doses (284, 568, 1136, 1420 mg / kg), while the average mean corpuscular hemoglobin (MCH), and the average mean corpuscular volume (MCV) at (P < 0.05) revealed a significant increase and at doses (568, 1136, 1420 mg/kg) after treatment with dry extract, at doses (1136, 1420 mg/kg) for the average of (MCH), at doses (568, 1136, 1420 mg/kg) for the average (MCV) after treatment with fresh extract, while the average Mean corpuscular hemoglobin concentration (MCHC) did not show any significant difference at (P < 0.05) after treatment with (fresh or dry) extract comparing with the control group.The parameters of blood in the intermediate duration of pregnancy showed a significant decrease at (P < 0.05) in the average of (Hb), (PCV) and (R.B.C.) at doses (284, 568, 1136, 1420 mg/kg) after treatment with fresh extract, while dry extract demonstrated a significant decrease in average of (Hb) and (PCV) at doses (568, 1136, 1420 mg/kg), and a significant decrease in the average of (R.B.C.) but at doses (284, 568, 1136, 1420 mg/kg), while the average of (MCH) and (MCV) showed a significant increase at (P < 0.05) and at doses (568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, while the average of (MCHC) did not showed any significant difference at (P < 0.05) after treatment with (fresh or dry) extract comparing with the control group.The parameters of blood in the final duration of pregnancy revealed a significant decrease (P < 0.05) in the average of (Hb), (PCV) and (R.B.C.) at doses (284, 568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, while (MCH) showed a significant increase at (P < 0.05) and at doses (568, 1136, 1420 mg/kg) after treatment with (fresh or dry) extract, (MCHC) did not demonstrated any significant difference at the level (P < 0.05) after treatment with (fresh or dry) extract at all doses, while (MCV) showed a significant increase at (P < 0.05) and at doses (284, 568, 1136, 1420 mg/kg) after treatment with fresh extract, and at doses (568, 1136, 1420 mg/kg) after treatment with dry extract comparing with the control group. The results showed a significant decrease at (P < 0.05) in the average of diameters of ovarian and corpus luteum after treatment with (fresh or dry) extracts in the early and final durations of pregnancy at doses (568, 1136, 1420 mg/kg), and in the intermediate duration after treatment with (fresh or dry) extract but at doses (284, 568, 1136, 1420 mg/kg) comparing with group control.As for the concentration of the hormone estrogen (? - estradiol) didn’t demonstrated a significant difference at (P < 0.05) during different durations of pregnancy after treatment with (fresh or dry) extract at all doses, whereas progesterone showed a significant decreased at doses (284, 568, 1136, 1420 mg/kg) in all durations after treatment with (fresh or dry) extract comparing with the control group. Using doses at (284, 568, 1136, 1420 mg/kg) with (fresh or dry) extracts revealed a significant increase at (P < 0.05) in the average number of infected follicles in the all durations of pregnancy comparing with the control group. The results showed that there are no significant difference in the lengths of the embryos during intermediate and final durations at (P < 0.05) after treatment with (fresh or dry) extract at all doses comparing with the control group.There are also histopathological changes in the ovary, uterus and placenta included degeneration in ovarian follicles, necrosis, congestion, hemorrhage, edema, vasodilatation and rupture in blood vessels at different using doses.These findings indicated that the treatment with (dry and fresh) extract at doses (568, 1136 or 1420) mg/ kg harmful on pregnancy in different durations (early, intermediate, and final) , also the using of fresh ginger more harmful than dry ginger.

التحري عن جينات المقاومة لمضادات البيتالاكتام واسعة الطيف في بكتريا Acinetobacter baumannii المعزولة من مصادر سريرية == Detection of Extended Spectrum Beta - Lactam Resistance Genes In Acinetobacter Baumannii Isolated From Clinical Sources

اسم المؤلف: صفا ماجد محمد الباجلاني
اسم المشرف: عباس عبود فرحان الدليمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: ديالى
الصفحات الاولى:
المستخلص: تضمنت الدراسة عزل وتشخيص 16عزلة من بكتريا Acinetobacter baumannii من اصل 196 عينة جمعت من مصادر سريرية مختلفة في مستشفى بعقوبة العام ومستشفى البتول التعليمي وكانت اعلى نسبة عزل لهذه البكتريا من مسحات الجروح %10.8، ومسحات الحروق بنسبة 8.3%، والادرار 6.9%، | The study included isolation and diagnosis of 16 isolates of Acinetobacter baumannii out of 196 samples collected from different clinical sources in Baquba General Hospital and Al - Batool Teaching Hospital. The highest rate for the isolation of these bacteria from wounds 10.8%, burns 8.3%, urine 6.9%, and blood 5%. The diagnosis of isolates was confirmed by ViTEK2 device in addition to phenotypic, microscopic characteristics and biochemical tests.The results of the investigation of virulence factors for Acinetobacter baumannii isolates showed that all isolates have the ability to adhesion surfaces of epithelial cells of humans (100%), while the ability of 13 isolates to produce biofilm was 81.2%, seven isolates had possessed efflux pumps with high efficiency (43.7%). The investigation of the sensitivity of the isolates for ten antibiotics, the results showed all isolates were resistant to antibiotic cephalexin was 100%, against antibiotics meropenem and imipenem was 50%, the other antibiotics resistance ratios ranged between 81.2% - 56.2%. The results of multi - drugs - resistance tests showed ten isolates of Acinetobacter baumannii possessed of multiple antibiotic resistance, the isolates were divided into two groups, resistance group and other sensitive depending on the resistance to antibiotics, as the first group included ten isolates resistance from 6 - 9 antibiotics, the second group included six isolates resisted from 1 - 4 antibiotics, and the first group is dominant in the study. The concentration (100 µg/ml) of cephalexin, cefotaxime, ceftriaxone to test the ability of isolates were resistant to cephalosporin antibiotics, all isolates showed resistance to this concentration for antibiotic Cephalexin, the resistance Cefotaxime, Ceftriaxone to this concentration was 75% for both antibiotics. The combination disk method used to investigate isolates producing for extended spectrum ? - lactamase enzyms and metallo ? - lactamase enzyms the results indicated that the percentage of isolates under study producing extended spectrum ? - lactamase enzymes and metallo ? - lactamase were 62.5% and 56.2% respectively from the total 16 isolation. It was determined the minimum inhibitory concentration MIC for cefotaxime and ceftazidime by method of multiplying serial concentrations, The results showed that the minimum inhibitory concentration for cefotaxime values ranging between 32 - 1024 µg/ml, as for the MIC ceftazidime his values ranging between 16 - 1024 µg/ml. Conducted process extract bacterial DNA for isolates of Acinetobacter baumannii, then held polymerize chain reaction PCR for isolates resistant to antibiotics ? - lactam and MIC values more than 64 µg/ml through the use of specialized primers that target specific sequence of genes blaCTX - m and blaSHV, relay outputs of the multiplication agarose gel concentration of 1%, the results showed that 5 isolates of Acinetobacter baumannii out of 10 isolates possessed gene blaCTX - m and by 50%, as for blaSHV gene the results showed that three of the isolates under study only has this gene and by 30%.

التاثيرات النسجية والدمية وبعض النواحي الوظيفية لعقار البيروكسيكام في ذكور الفئران البيض Mus musculus == Histological And Hematological Effects And Some Physiological Aspects of Piroxicam In Male Albino Mice Mus Musculus

اسم المؤلف: بشرى عمر احمد معروف
اسم المشرف: انتظار محمد مناتي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الحيوان - الانسجة
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: The current study aimed to detect the effect of Piroxicam in making behavioral, hematological and physiological changes in the kidneys of adult male albino mice Mus musculus, The present study also included measuring the diameters of glomeruli, proximal and distal convoluted tubules as well as the histopathological study.A total number of (128) adult male albino mice were utilized in this study, they were randomly distributed into four main groups, the first three groups were orally treated with concentration (50, 100, 150) mg/kg respectively, the forth group considered as control group were orally treated with (0.1) ml of distilled water for eight weeks, the samples were collected every two weeks for the histological study, blood samples were withdrawn for the hematological and physiological studies.The current study showed that there were behavioral changes observed on the experimental animals, represented by reluctance to food intake, lethargy, tremor, introversion, fast breathing, change in eye’s color and blackened feces, and the intensity of these symptoms increases with the increment of concentration and administration period.The statistical study results revealed that there was significant decrease at (P<0.05) in the mean numbers of red blood corpuscles, hemoglobin concentration and the percentage of packed cells volume in orally treated groups with (50, 100, 150) mg/kg for (6, 8) weeks of administration in comparison with control group, as for the white blood cells the statistical results for this study showed significant increase (P<0.05) in their mean number in treated groups with concentration (100, 150) mg/kg for (6, 8) weeks of administration compared to the control, whereas the groups treated with concentration of (50) mg/kg showed no significant changes along the administration period.Also all the concentrations showed significant differences (P<0.05) in the mean concentration of urea, creatinine and potassium ion in the blood serum of orally treated mice at the last weeks of administration in comparison with the control group.The statistical results of the present study also revealed that there were significant differences (P<0.05) in the mean diameters of renal glomeruli of the treated groups with concentration (50, 100, 150) mg/kg for eight weeks while there were non - significant differences in other periods, also there was non - significant decrease in the mean diameters of proximal convoluted tubules in the treated groups with concentration of (50, 100, 150) mg/kg in all periods except the period four weeks it showed a significant decrease (P<0.05) in the mean diameters of proximal convoluted tubules of the groups treated with the concentration (100, 150) mg/kg in comparison with control group. The statistical results also clarified that there were significant differences (P<0.05) in the mean diameters of distal convoluted tubules of the treated groups with concentration (100, 150) mg/kg for six and eight weeks of administration, while the group treated with concentration (50) mg/kg had no significant differences over the whole administration period.The study also recorded the presence of histopathological symptoms in the kidneys of treated mice with the dose (50)mg/kg represented by hemorrhage, vascular congestion, calcium casts formation inside the lumens of renal tubules, while the groups treated with (100)mg/kg of the drug showed a fibrin deposition inside renal tubular lumens, infiltration of inflammatory cells, cytoplasmic vacuolation in the epithelial cells of the renal tubules, and sloughing epithelium of renal tubules, while in the medullary region, there were epithelial cells atrophy in the renal tubules. The histological examination of mice kidneys treated with (150) mg/kg showed the appearance of hyalinization, shrinkage and complete loss of glomeruli, Pyknosis of nucleus and fibrosis around the blood vessels as well as the presence of oedema which occupied a large area of the kidney tissue

تقييم نوعية المياة الصناعية ووحدات المعالجة لمعمل النسيج القطني في الكاظمية == Evaluation of The Industrial Wstewater And Treatment In Al - Kadhimia Cotton Weaving Industry

اسم المؤلف: اسيل حسين محمد السعداوي
اسم المشرف: سهير ازهر الكاظمي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: البيئة - التلوث البيئي
الدرجة: ماجستير
الجامعة: جامعة بغداد
اللغة: العربية
مكان الجامعة: بغداد
الصفحات الاولى:
المستخلص: This study includes two axis, the first one determined the physical and chemical characteristics for several concentrations of industrial wastewater of cotton textile factory, that located in Al - Kadhmiya, which are disposable to the main assembly in the treatment unit (first unit) of the factory ; Also the final water quality was evaluated after the treatment in the fourth unite and compared with normal environmental averages (or compared with acceptable limits for discharged.The second axis of this study assessed the efficiency of the physical and chemical treatments in the third unit, and also the biological treatments in the fourth unit through detecting the removal percentage for each stage of the treatment to evaluated the treatment unit efficiency of this factory. Samples were collected from four treatment units for six month, single sample was collected per two weeks.The (local, field) tests were assessed for the collected samples including (Temperature, PH, DO and EC, while the laboratory tests included TSS, TDS, BOD, COD, oil and grease, So4 - 2 and heavy metals such as Pb, Zn and Cr).The results showed that the temperature values were with (normal range) acceptable limits (22.3C?) in the first unit and (19.8C?) in the fourth unit; PH values also were within normal limits in the almost treatment units (neutral), however, the electrical conductivity average was 3123?c/cm in the first unit, and 1101 ?c/cm in the fourth unit ; Moreover, the average values of COD were 580 mg/L in the first unit and 163 mg/L in the fourth unit. The removal percentage was 41% in the primary sedimentation in thesecond unit while it was 36% in the third unit for biological treatment.The BOD values were 487 and 302 mg /L in the first and fourth unit respectively ; the removal rates were 27% and 29% for primary sedimentation stage and biological treatment respectively and these rates are acceptable for normal limits.In general, the final treatment concentration was 302 mg/L in the fourth unit which is normal environmental rang, and also BOD concentration was not exceeded the environmental limits in the final stage of the treatment while the total percentage dissolved salts were 37% and 22% for the third unit which are normal in comparison to the Iraqi limits after the treatment for the fourth unit (703mg/L). Furthermore, the results of suspended solids found that the removal percentage was 76% for the second unit and 43%for the third unit while the total concentration of the last treatment was 61mg/L which is with normal limits, also the concentration of lipids of oils and grease was 0.8 mg/L for the fourth unit, while the removal percentage was efficient for the primary sedimentation and biological treatment. The heavy metals concentraitions were now with water and the main assembly tank and this was within normal environmental limits.

دور اجهاد المضاد الحياتي (سيفتازديم) في امراضية الممرضة البولية الايشريكية القولونية المنتجة للبيتا لاكتاميز موسعة الطيف في الفئران المختبرية == Role of Antibiotic (Ceftazidim) Stress On Pathogenicity of Uropathogenic Extended Spectrum ? - Lactamaes E. Coli In Laboratory Mice

اسم المؤلف: علي حسون حمادي عبد الله
اسم المشرف: محمد قيس العاني | حارث جبار فهد
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: الانبار
الصفحات الاولى:
المستخلص: جمع 60 نموذجا من عينات بول وسط المجرى من المرضى الراقدين في المستشفى الذين يعانون من اخماج المجاري البولية من مستشفى اليرموك ومستشفى الكرخ ومستشفى القديس رافائيل/ الراهبات للمدة من 27 شباط 2014 ولغاية 25 اذار 2014، وقد اظهرت نتائج الزرع البكتريولوجي الحصو | Sixty mid - stream urine specimens were collected from patients presented with urinary tract infections whom attending Al - Numan, Al - Yarmouk, Al - Karkh, and Saint Raphael/Al Rahibbat hospitals in Baghdad for the period February, 27th to March, 25th 2014. Bacteriological culture identified fifty (80.33%) isolates as E. coli depending on cultural and biochemical characteristics as well as Api and Vitek 2 automated systems. However, 82% of isolates developed ? - hemolysis on blood agar plates.Susceptibility of all isolates against seven commonly prescribed antibiotics were investigated and 96, 82, 84, 92, 92, and 56%. of isolates were resistant to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefalothin, imipenem, and meropenem, ceftazidime, respectively.Concomitantly, ? - lactamase production was detected phenotypically by disk replacement method. The results showed that 82% of all isolates have the ability to produce ? - lactamase. Interestingly, correlation coefficient between ? - lactamase and hemolysin production was 0.99.Using microtiter plate method, all E. coli isolates were tested for their ability to form biofilm. The results revealed that 36, 54, and 10% of isolates formed biofilm with variable thickness; weak, moderate, and strong, respectively.Alongside with previous findings, blaCTX - M - 2, blaVIM, blaTEM, and blaPER were identified in 18, 12, 10, and 4, respectively. Nevertheless, none of blaOXA - 1, blaOXA - 4, blaOXA - 30, blaIMP, blaCTX - M - 1, blaCTX - M - 9, blaKPC, and blaSHV. The isolate number 11 harboured the highest number of genes (blaTEM, blaVIM, and blaPER) among all tested isolates. Twelve albino mice was divided randomly into four groups comprising A through D injected with ceftazidime at sub MIC, E. coli 11, E. coli 11 with ceftazidime solution, and standard strain, respectively.Histopathological sections did not show any changes in respeuct to group A. however, group C suffered signs of infection less than those appeared in group B sections. Simultaneously, group D suffered intense histpathological changes more than other groups infected with resistant isolate.

دراسة علاقة سم Ochra. A بحالات الفشل الكلوي غير معروف الاسباب في محافظة القادسية == Study The Relationship of Ochra.A With Kidney Failure of Unknown Case In Al - Qadisiya Governorate

اسم المؤلف: هدى رحيم هاشم الموسوي
اسم المشرف: بهيجة عبيس حمود الخالدي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: دكتوراه
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: اجريت هذه الدراسة لتحديد العلاقة بين السموم الفطرية وحالات الفشل الكلوي غير معروف الاسباب لدى المرضى المراجعون الى مستشفى الديوانية التعليمي للفترة من اذار/2012 الى كانون الثاني/2013 والذين تم تشخيصهم من قبل الاطباء المختصون. اظهرت نتائج فحص عينات الادرا | This study was designed to detection the Relationship between some fungi and it’s toxin with Renal falier in patients suffer from Renal falier attended to Al - Diwanyia teaching hospital in period from March/ 2012 to January/ 2013, These patients were diagnosed by physician in above hospital. By using Thin Layer Chromatography (TLC) the results showed 23.07% from patients blood specimens had Ochratoxin A, 19.23% from patients urine specimens had Ochratoxin A 38.46% from patients had Ochratoxin A in blood and urine where only 59.61% your blood and urine were clear from above toxin, So healthy persons were had Ochratoxin A in your blood and urine by rate 20% 40% respectively and 10% from them had Ochratoxin A in your blood and urine and only 65% from them don’t had Ochratoxin A in their blood and urine.the concentration of Ochratoxin A in blood were ranged between 6.2 - 25.8 ?g/kg from weight boody and 4 - 10.3?g/kg in urine and in healthy reach concentration of Ochratoxin A in blood 2.3 - 3 ?g/kg from weight boody and 4.3 - 4.6 ?g/kg in urine.The data of this study showed the male highly infected 87.5% with ocratoxin while in female 70% and 71 - 80 years age had highly infected 92.85% with ochratoxin A. The results of study of biochemical parameter in patients showed highly raised in WBC and urea and Keratinin to reach to 12000 corpuscle /ml3 310 mg/dl, 12.2 mg /dl respectively and lowed the activity of alkaline phosphates enzyme and albumin into 2.2 u/l, 1.5 gm/dl, respectively and suger 120 mg /dl. The results of isolation and diagnosis by depending on Morphological, Microscopical and Biochemical characters showed there were two species of fungi belong to Aspergillus sp, include A. ochraceaus 25.42%, A. niger 12.71% and A. candidus 15.25% and Candida albicans 46.61% were isolated from urine patients where only C. albicans and C. krusi were isolated from blood of patient by percentage 66.66% and 33.33% respectively. So the results of single PCR method selected to detection the OCR and PSK genes in Aspergills spp which responsible for ochratoxin A production , showed 83.33% from A. ochraceaus isolates had OCR gene (907 bp) and 66.66% from A. nger had PSK gene (584bp)Also the results of this study showed A. ochraceus and A. niger had ability to produce metabolic compounds when grow on yeast - extract broth and these compounds had significant toxic effects(P?0.05) in biochemical parameters in albino rats represented raised number of white blood cells (WBCs) into 10610 cell/µm3, urea into 228 mg/dl and Keratinin into 15 mg/dl in blood of animals which treated with 5000 ?l/kg from metabolic products of above fungi respectively, comparative with control groups 10100 cell/µm3. in other side, these metabolic products caused in same dose lead to lower of albumin and activity of alkaline phosphatase enzyme into 2.8 mg/dl and 3.7 u/l respectively where normal level for this parameter in control groups.Histological study showed the dose of metabolic products 500 - 5000 µl/kg for both fungi caused pathogenic changes in kidney represented by, atrophy highly proliferation of cells in endothelial layer of Bowman's capsules in addition to hemorrhage and Focal agrgregate inflammatory cells.In other side, study of toxicity of ochratoxin A which extracted by using thin layer chromatography (TLC) teqnique from urine and blood of patients and yeast extract broth media that had growth of A. ochraceus and A. niger showed signification effects(P?0.05) for this compound in physiological and biochemical parameters also caused significant effects in histological parameters similar to these effects which occurred in previsious experiments while these effect don’t appear in control groups.

تحديد القابلية التمنعية لمستضد الفوعة لبكتريا (Vi) Chitosan المحملة على حبيبات Salmonella typhi وذيفان الكزاز في الجرذان == Determination of Immunogenicity For Virulence Antigen of Salmonella Typhi Antigen Conjugated To Chitosan And Tetanus Toxoid In Rats

اسم المؤلف: رغدة سعد محمد العمري
اسم المشرف: زياد متعب الخزاعي
الموضوع العام: علوم الحياة
السنة: 2015
الموضوع الدقيق: الاحياء المجهرية
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: القادسية
الصفحات الاولى:
المستخلص: This study was carried out during the period from October 2013 to September 2014. Salmonella typhi Samples were obtained from the Central Health Laboratory for the purpose of purification capsular polysaccharide antigen (Vi Ag). Capsular polysaccharide antigen was loaded on chitosan nanoparticle and tetanus toxoid. Recorded results showed that the antibody titers in (T1) Vi Ag + chitosan groups(620.8±364.8) were increased significantly than other groups, it included (T2) Vi Ag group (448±117.73), (T3) Vi + Tetanus toxoid group (537.6±142.53) and (T4) Vi Ag+ Ch. +TT group (121.6±38.4) at (P? 0.05).The documented results of total and differential WBCs counts were showed a significant increment in group (T1) (21020 ± 891.85 cell/1ml) compared with the control group (C) (5500 ± 230.94 cell/1ml) and other groups, it included (T3) group (10740 ± 980.61 cell/1ml), (T4) group (10650 ± 1217.78 cell/1ml) and (T2) group (9980 ± 572.18 cell/1ml) at (p? 0.05). on the other hand, the results of differential count showed elevation in percentage of neutrophils in group (T2) (40 ± 1.89%) compared with the control group (C) (33.3333 ± 0.66%) and other group, it included a (T3) group (33.6 ± 0.67%), (T4) group (34 ± 1.37%) and (T1) group (19.4 ± 0.66%) at (p? 0.05). Results also showed an increase in lymphocytes in the group (T1) (79.2 ± 2.7%) compared with the control group (58.3333 ± 1.76%) and other group, it included (T4) group (62 ± 2.06%), (T3) group (61.2 ± 2.72%) and (T2) group (55.8 ± 1.11%) at (p? 0.05).Also The study included determination the level of gene expression of two clusters of differentiation, CD25 and CD29 gene expression by RT - qPCR. The results of relative gene expression in CD25 gene showed in T4 (5.1936±2.17) were increased significantly than other groups, it included T1 (2.5604±0.64), T2 (3.7032±0.54) and T3 (4.4739±1) at (P? 0.05). The results of relative gene expression in CD29 gene which showed T3 (162.3256±89.52), increased significantly than other groups, it included T1 (33.8276±16.6), T2 (59.6817±26.65), and T4 (37.9605±3.09) at (P? 0.05).This findings were supported by the increment activity determined in lymph nodes of spleen & thymus which increased in size and numbers with active germinal centers. The overall results indicated that the use of chitosan nano particles and tetanus toxoid can enhance and improve the immune response against a given vaccines.

دراسة لتشخيص بكتريا الملوية البوابية Helicobacter pylori المعزولة من الخزع النسيجية والغائط == Study For Diagnosis Helicopacter Pylori Isolated From Biopsy And Stool

اسم المؤلف: ابرار علي حسين
اسم المشرف: هيام عبد الرضا كريم العواد | ياسمين خضير الغانمي
الموضوع العام: علوم الحياة
السنة: 2015
الدرجة: ماجستير
اللغة: العربية
مكان الجامعة: كربلاء
الصفحات الاولى:
المستخلص: تناولت الدراسة الحالية عزل وتشخيص بكتريا (Helicobacter pylori) من الخزع النسيجية وعينات البرازالماخوذة من المرضى المراجعين مستشفى الحسين التعليمي حيث جمعت 122 عينة من الخزع النسيجية من وحدة الناظور من 61 مريض بواقع عينتين للمريض الواحد, كما تم جمع 228 عين | The present study addressed isolate and diagnose the bacteria (Helicobacter pylori) from biopsy and stool, samples taken from AL - Hussein Teaching Hospital where collected 122 samples of biopsy from the endoscopy. From 61 patients by two samples per patient, also were collected 228 samples of stool from the laboratory of the Department of parasites by two samples of 114 patients for the period from January 2014 to January 2015. Where shown on biopsy results which were subject to examination by the rapid urease test (RUT) the presence of 60 (98.4%) cases, while there were 23 (37.7%) case of those bacteria being positive related to biopsy was not significant differences between the two tests performed. When the distribution of test results (RUT) and culture according to age group, it appear that the highest percentage of infection was in 50 - 41 age group that which showed the highest percentage on examination (RUT) of the number of infected at 16 (26.23%) cases, either in the culture technique number of infected at 9 (14.75%) cases. But when the results of each of the examination (RUT) and culture were distribution according to the sex of the patient's infection was the higher in the females than males reaching males 28 (45.90%) cases in females of 32 (52.46%) cases for examination (RUT). No significant differences appear between the sexes in terms of infected. While the males in 8 (13.11%) and in the case of females was 15 (24.59%) case for the culture technique it did not show significant differences between the sexes in terms of the patients. While taking stool samples to check the stool antigens, it was found that there are 76 (66.7%) cases diagnosed while the technology for the polymerase chain reaction gene cagA to 49 (43%) cases have significant differences between the tests performed. When distribution the results of each of the stool antigens and polymerase chain reaction technique by age group category the highest infected was in 46 - 55 the age group with the number of 23 (20.18%) people case for screening stool antigens. The test results of the technology polymerase chain reaction showed that highest rate of infection was in the 35 - 26 age group where the number of patients was at 15 (13.16%) case.The results of the stool antigens examination also showed that the infection rate among males was higher than females, 39 males (34.21%) and in the case of females has reached the number of 37 cases (32.46%) casesThat did not show significant differences between the sexes patient. The technical results of the polymerase chain reaction by sex has been the injury was higher in females than males reaching 22 in males (19.30%) cases, while in females was 27 (23.68%) cases did not show significant differences between the sexes in terms of the infected. The results of the study conclude that (RUT) and stool antigen test is the fastest in the detection of infection from the bacteria H. pylori and then comes beyond culture technique for biopsy sample and then polymerase chain reaction for stool samples
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