Author name:
سارة هاشم داخل الحلي
Supervisor name:
محمد صبري عبد الرزاق السعيد | جواد كاظم الخفاجي
Abstract:
يهدف هذا البحث الى دراسة بكتريا المكورات العنقودية السلبية التخثر, وشملت الدراسة جمع120 عينة ادرار من المرضى المصابين بالتهاب المجاري البولية من الاشخاص المراجعين والراقدين في مستشفى بابل للنسائية والاطفال ومستشفى الحلة التعليمي وللفترة من كانون الاول 201 | This work aims to study coagulase - negative Staphylococci, which included 120 urine sample were obtained from patients suffering from urinary tract infection; who attained to Babylon Maternity and Pediatrics Hospital, and Al - Hilla General Teaching Hospital, during the period from December 2015 to March 2016), they were diagnosed as having UTI by the Urologists. The samples were analyzed for any signs of infection and immediately inoculated on blood and MacConkey agar media plates. All plates were incubated aerobically at 37 ?C for 24 - 48 hrs.Results of morphological and biochemical characterization revealed that out of total of 120 urine samples, 110 showed positive culture, among them 37 isolates are belonged to coagulase - negative Staphylococci, other 73 isolates belonged to other bacterial genera.Molecular detection of coagulase gene (coa) was done for all CoNS isolates. The results showed that 28/37 isolates (75.67%) were positive for this marker, distributed as follows : S. epidermidis gave 15 isolates, S. saprophyticus gave 9 isolates and S. haemolyticus gave 4 isolates.Also, molecular investigation of fnb A and B genes are carried out for all CoNS isolates by using specific PCR markers. 13/37 isolates (35%) were positive for fnb A gene, as 6/13 of S. epidermidis, 5/13 of S.saprophyticus and 2/13 of S. haemolyticus have this gene in their genome. On the other hand, only 4/37 isolates gave positive result for fnb B gene; as 2 isolates of both S. saprophyticus and S. epidermidis have this gene in their genome.Moreover, sec gene also was investigated in CoNS isolates. The results showed that all CoNS isolates are free from this gene.Regarding urease enzyme production, S. epidermidis and S.saprophyticus isolates were found to be urease positive, while S.haemolyticus isolates display urease negative.To demonstrate the ability of bacteria to form struvite stone, urine samples obtained from healthy individuals were used. It was found that struvite stone was formed by S. epidermidis and S. saprophyticus isolates.Moreover, susceptibility to 10 antibiotics were tested by using disc diffusion test (DDT). S. epidermidis was showed 100% resistance for each Methicilline, Ceftriaxone, Ceftazidime and Cefoxitin. Also, it show high resistance 94%, 88%, 88%, 76%, and 59% for Impenem, Gentamycin, Pipracillin, Ciprofloxacin and Amikacin respectively. However, S. epidermidis showed low resistance (6%) for Vancomycin. So, all S. saprophyticus isolates showed high resistance (100%) to each Pipracilline, Ceftriaxone, Methicillin, Ceftazidime and Ciprofloxacin. It showed high resistance (92%) for each Vancomycin and Imipenem, and (83%), (75%), (75%) for Cefoxitin, Gentamycin and Amikacin respectively. In addition to that, all S. haemolyticus was showed (100%) resistance for each Ceftriaxone, Pipracillin, Methicilline, Ceftazidime and Cefoxitin. Also, showed high resistance (75%), (63%) and (50%) for Gentamycin, Amikacin and Ciprofloxacin respectively. While it was showed low resistance (13%) to Vancomycin.
