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دراسة الملف المصلي وتقييم بعض الخصائص المناعية لدى مرضى الحزام الناري == Serum Profile Study And Evaluation Of Some Immune Features Among Patients With Shingles
Author name:
زينب عبد النبي طليفح النصراوي
Supervisor name:
حبيب صاحب نهر | محمد كاظم طاهر الحطاب
General topic:
Medicine
Specific topic:
Microbiology
Degree:
Doctorate
University:
University of Babylon - Faculty Of Medicine - Department Of Microbiology
Language:
English
University location:
Babylon
First pages:
19T1205 - p.pdf
Abstract:
اجريت الدراسة الحالية للفترة من 1شباط 2015 لغاية 3شباط 2016 وجمعت خلالها عينات المرضى المصابين بالحزام الناري من قسم الامراض الجلدية في العيادات الاستشارية في مستشفى مرجان التعليمي للتحري عن بعض الجوانب المناعية في المرضى, وكان العدد الكلي للمرضى 50 مريضا | This study was conducted during the period from 1st February 2015 to 3rd February 2016, and the samples were collected from dermatology department of the consultant clinic in Marjan Teaching Hospital to investigate certain immunological markers of patients with shingles. The total number of patients were whose their ages ranges were 14 - 80 years. Age group (40 - 61) years have the highest percentage of infection reaching 46%.The immunofluorescence test for Varicella Zoster virus - specific IgG showed 48 out 50 are positive(96%). The higher percentage of infection with shingles was in female (54%), while male patients the percentage was(46%).The results showed that patients with blood group (O+) have 50% of infection rate.When CD4 was investigated by ELISA test , the results showed that the mean± SD of serum level of CD4 in shingles patients and control group is (6.70± 0.97 and 9.36±2.02) ng/ml respectively. The concentration of CD4 was lower in patients with shingles than healthy control group, while concentration of CD8 and CD56were higher than healthy control group ( the mean± SD of serum level of CD8 in shingles patients and control group was 21.42±5.43 and19.11±3.29 ng /ml respectively, whereas the mean ± SD of CD56 concentration in shingles and control group were 107.58± 40.39 and 67.59 ± 36.31 ng/ml, respectively).The results also showed that the concentrations of IFN ? and TNF? were lower in patients with shingles compared with non - infected persons , as the following : the mean± SD of serum level of IFN ? in shingles patients and control group was 184.31±21.95 and 218.03±26.21 pg /ml respectively, and the mean ± SD of TNF? concentration in shingles and control group were 51.55± 5.14 and 62.35 ± 6.74 pg/ml, respectively. For IL 10, the concentration was higher in patients with shingles than that in the healthy control group; the mean± SD of serum level of IL 10 in shingles patients and control group was12.42±5.59 and 4.47±0.90 pg /ml respectively. This study was concluded following : • Gender represents a risk factor for the occurrence of shingles mainly at ages over 40 years old (higher in females than males).• Varicella Zoster virus specific IgG is a sensitive and specific test that can be used for the laboratory diagnosis of shingles.• Varicella Zoster virus reactivation has a suggested link with decreased level of soluble CD4 molecules and, for lesser extend with increased soluble CD8 and CD56 molecules in patients serum. • Blood group O+ might be consider as a risk factor for reactivation of VZV infection as the majority of patients were within this blood group.• A diminished role of the inflammatory TH1 cells in reactivated shingles patients is more likely as evident by the reduced concentration of IFN? and TNF? profiles. The Treg cells (CD4+ CD25+) seems to play a role in such reduction of TH1 proinflammatory cytokines as evident by the significant elevation of IL10 in shingles patients.
